CN114164100A - Environmental protection aerobic bacteria cultivates and uses hybridization appearance - Google Patents

Abstract

The invention discloses an environment-friendly hybridization instrument for aerobic bacteria culture, which relates to the field of bacteria hybridization instruments and comprises a fixed base and a hydraulic rod outer cylinder, wherein an inner rod of a hydraulic rod matched with the hydraulic rod outer cylinder is connected inside the hydraulic rod outer cylinder in a sliding manner, the top of the fixed base is rotatably connected with a rotating column, a plurality of groups of lotion boxes and a plurality of groups of bearing tables are arranged outside the rotating column, and the top of each bearing table is movably connected with an NC membrane bearing plate. According to the invention, the outer cylinder of the hydraulic rod is matched with the inner rod of the hydraulic rod to drive the sliding column to move up and down, and further drive the rotating column to rotate, so that the liquid washing box and the bearing table rotate in a matched manner while the liquid dropping needle cylinder and the electric heating drying rod move up and down, and the liquid washing box and the bearing table rotate, so that the liquid dropping needle cylinder automatically dropwise add, and the liquid dissolving liquid dropwise added to the top of the NC membrane bearing plate is dried through the electric heating drying rod, so that the operation procedures of experimenters are reduced, and the experimenters can complete preparation work only by taking and placing the NC membrane, and the experimental efficiency is improved.

Description

Environmental protection aerobic bacteria cultivates and uses hybridization appearance

Technical Field

The invention relates to the field of bacteria hybridization instruments, in particular to a hybridization instrument for culturing environment-friendly aerobic bacteria.

Background

Colony in situ hybridization is another form of in situ hybridization technique, commonly used to identify foreign DNA inserts homologous to probes in bacterial colonies with recombinant molecules, the bacterial colonies are first transferred from a petri dish to a nitrocellulose membrane, lysed, and then subjected to DNA release, denaturation, and immobilization before hybridizing to probes, and a similar technique is plaque in situ hybridization, except that phage are blotted on the nitrocellulose membrane instead of colonies, which is specifically used to screen gene libraries or cDNA libraries for recombinants homologous to probes.

According to a technique for molecular genetic research, when a double-stranded nucleic acid molecule is heated to eighty to one hundred degrees centigrade, hydrogen bonds between base pairs are broken to form two single strands, which is called denaturation or melting of the nucleic acid, the denatured nucleic acid molecule is slowly cooled, and a double-stranded molecule is reformed between complementary base pairs, which is called renaturation or annealing of the nucleic acid, and the operation is generally performed by dropping on an NC film.

However, the existing environment-friendly aerobic bacteria culture hybridization is mostly operated by pure hands of experimenters, the experimenters suck the treated bacteria dissolving liquid through a dropper, then the liquid is dripped on an NC membrane, and then the dripped bacteria dissolving liquid NC membrane is placed under a dryer for heating treatment at eighty to one hundred degrees centigrade.

Disclosure of Invention

Based on the above, the invention aims to provide the hybridization instrument for culturing the environment-friendly aerobic bacteria, so as to solve the technical problems of low pure manual operation efficiency and troublesome cleaning and disinfection operation in the conventional hybridization culture of the environment-friendly aerobic bacteria.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides an aerobic bacteria of environmental protection cultivates and uses hybridization appearance, includes unable adjustment base and hydraulic stem urceolus, the inside sliding connection of hydraulic stem urceolus has pole rather than the complex hydraulic stem in, the unable adjustment base top is rotated and is connected with the rotation post, the rotation post outside is provided with multiunit lotion box and multiunit plummer, plummer top swing joint has NC membrane loading board, hydraulic stem urceolus bottom is provided with the air pump dustcoat pole bottom in the hydraulic stem be provided with air pump dustcoat sliding connection's slip post, slip post bottom with be connected with slewing mechanism between the rotation post, the slip post outside is connected with the dropping liquid device through fixed establishment.

Through adopting above-mentioned technical scheme, the pole cooperation in the hydraulic stem urceolus and the hydraulic stem of setting drives the slip post and reciprocates, and then drives the rotation post and rotate, drives lotion box and plummer through the rotation post and rotates, bears the weight of the NC membrane through NC membrane loading board, drives the rotation cover through the slip post and reciprocates.

The invention is further provided that the lotion box and the bearing platform are arranged at intervals, the lotion box is higher than the bearing platform, and the lotion box is internally provided with needle washing liquid.

Through adopting above-mentioned technical scheme, the interval arrangement that sets up carries out dropwise add bacteriolysis and abluent operation in proper order when making the dropping liquid cylinder reciprocate, bears the NC membrane loading board through the plummer, carries out surface cleaning to the dropping liquid cylinder through the liquid of washing the needle.

The invention is further arranged in such a way that a groove matched with the NC film bearing plate is arranged at the top end of the inner wall of the bearing table, and the top of the bearing table and one side far away from the rotating column are of open structures.

Through adopting above-mentioned technical scheme, the recess that sets up makes the plummer can fix the NC membrane loading board, makes things convenient for the experimenter to take and place the NC membrane loading board through opening the structure.

The invention is further provided with a pair of outer beams arranged outside the sliding column, a liquid dropping needle cylinder matched with the NC membrane bearing plate is arranged at the bottom of one of the outer beams, and an electric heating drying rod matched with the liquid washing box and the bearing table is connected at the bottom of the other outer beam through a telescopic structure.

Through adopting above-mentioned technical scheme, the fixed dropping liquid cylinder of outer beam and the electric heat of setting are dried the stick, drip the bacteriolysis liquid to NC membrane loading board through the dropping liquid cylinder, toast the bacteriolysis liquid on the NC membrane loading board through the electric heat stoving stick to carry out the surface degerming to the lotion in the lotion box.

The electric heating drying rod is further provided with a limiting sleeve which is fixed with the outer beam in a sleeved mode at the upper end of the outer side of the electric heating drying rod, and a spring is connected between the top of the electric heating drying rod and the top end of the inner portion of the limiting sleeve.

By adopting the technical scheme, the moving direction of the electric heating drying rod is limited by the limiting sleeve, and the electric heating drying rod can stretch and retract through the spring.

The invention is further provided that the heating end of the electric heating drying rod faces downwards, and a clamping plate matched with the lotion box is arranged at the position, above the heating end, outside the electric heating drying rod.

By adopting the technical scheme, the clamping plate controls the descending amount of the electric heating drying rod, so that the electric heating drying rod can be close to the liquid level of the washing liquid in the washing liquid box, and the electric heating drying rod keeps the distance from the top of the NC membrane bearing plate to dissolve bacteria liquid, thereby controlling the applied temperature.

The invention is further provided with a rotating sleeve movably connected with the rotating column at the bottom of the sliding column, a plurality of groups of rotating chutes are arranged on the outer side of the rotating column, bulges matched with the rotating chutes are arranged on the inner wall of the rotating sleeve, and the rotating chutes are composed of upper-section vertical grooves, ascending chutes, descending chutes and lower-section vertical grooves.

By adopting the technical scheme, the arranged rotating sleeve moves up and down to drive the protrusion to move, so that the protrusion moves in the rotating chute and drives the rotating column to rotate, the protrusion presses the lower bottom of the descending chute when descending, thereby driving the rotating column to rotate anticlockwise, when the bulge moves to the lower section vertical groove, the rotating column stops moving, so that only vertical displacement occurs between the dropping liquid needle cylinder and the electric heating drying rod as well as the washing liquid box and the NC membrane bearing plate, thereby ensuring the operation precision, because of the opening position of the ascending chute, the bulge can contact the top of the ascending chute, and further push the rotating column to continue rotating anticlockwise, and then into the upper vertical slot, the protrusions again squeeze the bottom of the drop chute as they drop and leave the upper vertical slot, thereby causing the rotating post to rotate continuously and form a loop.

The invention is further provided that the top of the fixed base is provided with a glass outer cover, the top of the glass outer cover is provided with a top shell, and an air filter is arranged in the top shell.

Through adopting above-mentioned technical scheme, the glass dustcoat that sets up makes inside and outside separate to make things convenient for the experimenter to observe, protect inside electric assembly through the top shell, filter equipment inside air through air cleaner, the top shell outside is provided with operating panel.

The glass outer cover is hinged with an operation door, and is controlled through an operation panel.

Through adopting above-mentioned technical scheme, the ventilation board guide air that sets up passes, discharges and the inhaled air through the vent, through the air pipe guide air flow direction, through the circulation direction of check valve restriction air, and then cooperation air pump dustcoat and slip post extract and release the air, open and close through the operation door.

In summary, the invention mainly has the following beneficial effects:

1. according to the invention, the outer cylinder of the hydraulic rod is matched with the inner rod of the hydraulic rod to drive the sliding column to move up and down, so that the rotating column is driven to rotate, the washing liquid box and the bearing table rotate in a matched manner while the liquid dropping needle cylinder and the electric heating drying rod move up and down, so that the liquid dropping needle cylinder automatically drips, and the liquid dissolving liquid dripped at the top of the NC membrane bearing plate is dried by the electric heating drying rod, so that the operation procedures of experimenters are reduced, the experimenters can finish preparation work only by taking and placing the NC membrane, and the experimental efficiency is improved;

2. according to the invention, the sliding column moves up and down, so that the liquid dropping needle cylinder is automatically immersed into the lotion box for cleaning after dropping liquid on the NC membrane bearing plate, and the surface layer of the lotion in the lotion box is sterilized by the electric heating drying rod, thereby avoiding the defect that the traditional hybridization culture experiment needs frequent manual sterilization.

Drawings

FIG. 1 is a structural cross-sectional view of the present invention;

FIG. 2 is an enlarged view of the structure of FIG. 1 according to the present invention;

FIG. 3 is a schematic view of the main body of the present invention;

FIG. 4 is a schematic view of the door opening of the present invention;

FIG. 5 is an internal structural view of the present invention;

fig. 6 is an internal cross-sectional view of the present invention.

In the figure: 1. a fixed base; 2. a vent; 3. a ventilation board; 4. a glass housing; 5. a vent pipe; 6. an air filter; 7. an outer cylinder of the hydraulic rod; 8. an air pump housing; 9. an inner rod of the hydraulic rod; 10. a sliding post; 11. an outer beam; 12. a limiting sleeve; 13. a spring; 14. a washing liquid box; 15. an NC membrane carrier plate; 16. a bearing table; 17. rotating the chute; 1701. an upper vertical slot; 1702. a rising chute; 1703. a descending chute; 1704. a lower vertical trough; 18. an electric heating drying rod; 19. rotating the column; 20. a protrusion; 21. a liquid dropping needle cylinder; 22. a clamping plate; 23. rotating the sleeve; 24. a top housing; 25. the door is operated.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

The following describes an embodiment of the present invention based on its overall structure.

An environment-friendly hybridization instrument for culturing aerobic bacteria, as shown in figures 1 and 2, comprises a fixed base 1 and a hydraulic rod outer cylinder 7, wherein the hydraulic rod outer cylinder 7 is connected with a hydraulic rod inner rod 9 matched with the hydraulic rod outer cylinder 7 in a sliding manner, the hydraulic rod outer cylinder 7 and the hydraulic rod inner rod 9 push a sliding column 10 to move downwards through the working principle of a hydraulic rod, the sliding column drives an outer beam 11 to move downwards when moving downwards, and further drives a dropping liquid syringe 21 to move downwards and approach an NC membrane bearing plate 15, the top of the fixed base 1 is connected with a rotating column 19 in a rotating manner, the rotating column 19 drives a washing liquid box 14 and a bearing table 16 to rotate, the outer side of the rotating column 19 is provided with a plurality of groups of washing liquid boxes 14 and a plurality of groups of bearing tables 16, the top of the bearing tables 16 is movably connected with an NC membrane bearing plate 15, an NC membrane is borne by the NC membrane bearing plate 15, the bottom of the hydraulic rod outer cylinder 7 is provided with an air pump outer cover 8, the bottom of the hydraulic rod inner rod 9 is provided with the sliding column 10 connected with the air pump outer cover 8 in a sliding manner, the rotating sleeve 23 is driven to move up and down through the sliding column 10, the sliding column 10 is driven to move up and down, the rotating column 19 is driven to rotate, a rotating mechanism is connected between the bottom of the sliding column 10 and the rotating column 19, and a liquid dropping device is connected to the outer side of the sliding column 10 through a fixing mechanism.

Referring to fig. 5 and 6, thewashing liquid box 14 and thebearing platform 16 are arranged at intervals, and the drippingliquid syringe 21 is moved up and down through the arranged interval arrangement to perform operations of dripping, dissolving bacteria and cleaning in sequence, thewashing liquid box 14 is higher than the bearingplatform 16, thewashing liquid box 14 is internally provided with the needle washing liquid, the drippingliquid syringe 21 is immersed in thewashing liquid box 14, and the drippingliquid syringe 21 is immersed in thewashing liquid box 14 to achieve a cleaning effect.

Referring to fig. 1 and 4, a groove matched with the NCfilm carrier plate 15 is formed at the top end of the inner wall of the carrier table 16, the carrier table 16 can fix the NCfilm carrier plate 15 through the groove, the top of the carrier table 16 and the side far away from the rotatingcolumn 19 are open structures, and when taking the carrier table, a hand can be inserted into the carrier table 16 to directly lift the NC film and the NCfilm carrier plate 15 upwards to integrally support the NC film and avoid the NC film from being polluted by bacteria on the hand.

Referring to fig. 2, a pair ofouter beams 11 are arranged outside a slidingcolumn 10, the sliding column drives theouter beams 11 to move downwards when moving downwards, and further drives a liquid droppingneedle cylinder 21 to move downwards and approach to an NCfilm bearing plate 15, a liquid droppingneedle cylinder 21 matched with the NCfilm bearing plate 15 is arranged at the bottom of one of the pair ofouter beams 11, the liquid droppingneedle cylinder 21 drops a solution for dissolving bacteria to the NC film after approaching to the NC film, meanwhile, a limitingsleeve 12 also descends and drives an electricheating drying rod 18 to descend, the bottom of the otherouter beam 11 is connected with an electricheating drying rod 18 matched with aliquid washing box 14 and a bearing table 16 through a telescopic structure, and the electricheating drying rod 18 is close to the NC films of other stations and dries the NC films.

Referring to fig. 2, the upper end of the outer side of the electricheating drying rod 18 is movably sleeved with a limitingsleeve 12 fixed with theouter beam 11, the moving direction of the electricheating drying rod 18 is limited by the limitingsleeve 12, aspring 13 is connected between the top of the electricheating drying rod 18 and the top end of the inner part of the limitingsleeve 12, and the electricheating drying rod 18 can stretch and retract through thespring 13.

Referring to fig. 2, the heating end of the electricheating drying rod 18 faces downward, a clampingplate 22 matched with thelotion box 14 is arranged above the heating end at the outer side of the electricheating drying rod 18, the descending amount of the electricheating drying rod 18 is controlled by the clampingplate 22, so that the electricheating drying rod 18 can be close to the liquid level of the lotion in thelotion box 14, the electricheating drying rod 18 keeps a distance from the top of the NC film bearing plate to dissolve the bacteria liquid, and the applied temperature is controlled.

Referring to fig. 2, a rotating sleeve 23 movably connected to a rotating column 19 is disposed at the bottom of a sliding column 10, a plurality of sets of rotating chutes 17 are disposed at the outer side of the rotating column 19, the rotating sleeve 23 moves up and down to drive a protrusion 20 to move, so that the protrusion 20 moves in the rotating chute 17 and drive the rotating column 19 to rotate, the inner wall of the rotating sleeve 23 is provided with a protrusion 20 matched with the rotating chute 17, the rotating chute 17 is composed of an upper vertical slot 1701, a rising chute 1702, a falling chute 1703 and a lower vertical slot 1704, the protrusion 20 presses the lower bottom of the falling chute 1703 when falling to drive the rotating column 19 to rotate counterclockwise, the rotating column 19 stops moving when the protrusion 20 moves to the lower vertical slot 1704, so that only vertical displacement occurs between the dripping needle cylinder 21 and the electric heating drying rod 18 and the lotion box 14 and the NC film carrier plate 15, thereby ensuring operation accuracy, when the protrusion 20 rises and leaves the lower vertical slot 1704, due to the open position of the ascending chute 1702, the protrusion 20 will contact the top of the ascending chute 1702, thereby pushing the rotating column 19 to continue rotating counterclockwise and then enter the upper vertical slot 1701, and the protrusion 20 will again press the bottom of the descending chute 1703 when descending and leaving the upper vertical slot 1701, thereby causing the rotating column 19 to rotate continuously and form a loop.

Please refer to fig. 1 and 5, a glassouter cover 4 is arranged on the top of the fixingbase 1, the inside and the outside are separated through the arranged glassouter cover 4, and the observation of experimenters is facilitated, atop shell 24 is arranged on the top of the glassouter cover 4, the inside electric assembly is protected through thetop shell 24, anair filter 6 is arranged inside thetop shell 24, the sucked air is pushed into theair filter 6 through a one-way valve, and then enters another ventilatingplate 3 from another ventilatingpipe 5, and is discharged through aventilating opening 2, so that the air filtering operation is automatically executed once after the completion of drying, dripping and disinfection at each time, and the air quality inside the equipment is ensured to meet the experimental requirements of the composite.

Please refer to the figure, a pair ofventilation plates 3 is connected between the glass outer covers 4, the inner walls of theventilation plates 3 are provided withventilation openings 2,ventilation pipes 5 are connected between theventilation plates 3 and theair filter 6, a pair of air passing pipes is connected between theair filter 6 and the air pumpouter cover 8, a pair of check valves with opposite directions are arranged inside the air passing pipes, when the slidingcolumn 10 descends inside the air pumpouter cover 8, negative pressure is formed inside the air pumpouter cover 8, then theventilation pipes 5 connected with theair filter 6 are matched with the check valves, the air inside the equipment is sucked from theventilation openings 2, when the slidingcolumn 10 ascends in the air pumpouter cover 8, the sucked air is pushed into theair filter 6 through the check valves, the glass outer covers 4 are hinged withoperation doors 25, the outer side of the topouter cover 24 is provided with an operation panel, and the equipment is operated through the operation panel.

The working principle of the invention is as follows: firstly, an external power supply is switched on, lotion is added into alotion box 14, a liquid droppingneedle cylinder 21 is installed, anoperation door 25 is opened, an NC membrane is placed at the top of an NCmembrane bearing plate 15, and equipment is operated through an operation panel, at the moment, a hydraulic rodouter cylinder 7 and a hydraulic rodinner rod 9 push a slidingcolumn 10 to move downwards through the working principle of a hydraulic rod, the sliding column drives anouter beam 11 to move downwards when moving downwards, and further drives the liquid droppingneedle cylinder 21 to move downwards and approach the NCmembrane bearing plate 15, the liquid droppingneedle cylinder 21 drips lysozyme to the NC membrane after approaching is used, meanwhile, a limitingsleeve 12 also descends and drives an electricheating drying rod 18 to descend, and the electricheating drying rod 18 is close to NC membranes of other stations and dries the NC membranes;

thebulge 20 moves in therotating chute 17 and drives therotating column 19 to rotate, thebulge 20 presses the lower bottom of the descendingchute 1703 when descending, and further drives therotating column 19 to rotate anticlockwise, when thebulge 20 moves to the lowervertical groove 1704, the rotatingcolumn 19 stops moving, further only vertical displacement occurs between the droppingliquid syringe 21 and the electricheating drying rod 18, thelotion box 14 and the NCmembrane bearing plate 15, so that the operation precision is ensured, when thebulge 20 rises and leaves the lowervertical groove 1704, due to the opening position of the ascendingchute 1702, thebulge 20 can contact the top of the ascendingchute 1702, further the rotatingcolumn 19 is pushed to rotate anticlockwise continuously, and then enters the uppervertical groove 1701, when thebulge 20 descends and leaves the uppervertical groove 1701, the bottom of the descendingchute 1703 can be squeezed again, so that therotating column 19 rotates continuously, and a circulation is formed;

according to the experimental requirement of the lysis liquid, a bracket can be added on the top of the NCmembrane bearing plate 15, the clampingplate 22 is supported by the bracket, so that the electricheating drying rod 18 cannot further descend when descending to the bracket, the distance between a heat source and the lysis liquid is increased, the temperature for extracting and baking the lysis liquid is reduced, and the baking temperature meets the experimental requirement;

after drying and dripping are completed, when theinner rod 9 of the hydraulic rod drives the slidingcolumn 10 and therotating sleeve 23 to ascend, the electricheating drying rod 18 and the liquid droppingneedle cylinder 21 are driven to ascend, theliquid washing box 14 and the NCmembrane bearing plate 15 are driven to rotate at the same time, when the electricheating drying rod 18 and the liquid droppingneedle cylinder 21 descend again, the liquid droppingneedle cylinder 21 is immersed in theliquid washing box 14, the liquid washing in the liquid droppingneedle cylinder 21 is immersed by the liquid washing to achieve a cleaning effect, meanwhile, the electricheating drying rod 18 is close to the liquid level of the liquid washing in the otherliquid washing box 14 under the limit of the clampingplate 22, meanwhile, the clampingplate 22 covers the top of theliquid washing box 14 to form sealing, at the moment, the electricheating drying rod 18 is close to the liquid level of the liquid washing and is sealed by the clampingplate 22, and continuous high-temperature sterilization can be carried out on the surface of the liquid washing at higher temperature;

during sterilization, theoperation door 25 can be opened to carry out the placing of the NC membrane of the next batch and the taking operation of the finished NC membrane, and during the taking, a hand can be inserted into the bearing table 16 to directly lift the NC membrane and the NCmembrane bearing plate 15 upwards to integrally support the NC membrane, so that the NC membrane is prevented from being polluted by bacteria on the hand;

when the operatingdoor 25 is opened, the program setting of the operating panel is triggered, the lifting operation of the hydraulic rodouter cylinder 7 and the lifting operation of the hydraulic rodinner rod 9 are forbidden, and the accidental injury to a person is avoided;

slidingcolumn 10 is when the inside decline ofair pump dustcoat 8, the inside negative pressure that forms ofair pump dustcoat 8, and then theventilation pipe 5 through connectingair cleaner 6 and the cooperation of check valve, inhale equipment inside air fromvent 2, when slidingcolumn 10 rises inair pump dustcoat 8, pushair cleaner 6 with inspiratory air cooperation check valve, and then get into another ventilatingboard 3 from anotherventilation pipe 5, and discharge throughvent 2, thereby at every turn dry, drop-wise and disinfect and accomplish back automatic execution primary air filtration operation, guarantee the compound experimental requirement of equipment inside air quality.

Although embodiments of the present invention have been shown and described, it is intended that the present invention should not be limited thereto, that the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples, and that modifications, substitutions, variations or the like, which are not inventive and may be made by those skilled in the art without departing from the principle and spirit of the present invention and without departing from the scope of the claims.

Claims (9)

1. The utility model provides an environmental protection aerobic bacteria cultivates and uses hybridization appearance, includes unable adjustment base (1) and hydraulic stem urceolus (7), pole (9) in hydraulic stem urceolus (7) inside sliding connection has rather than complex hydraulic stem, its characterized in that: unable adjustment base (1) top is rotated and is connected with rotation post (19), it is provided with multiunit lotion box (14) and multiunit plummer (16) to rotate post (19) outside, plummer (16) top swing joint has NC membrane loading board (15), hydraulic stem urceolus (7) bottom is provided with air pump dustcoat (8) pole (9) bottom is provided with air pump dustcoat (8) sliding connection's slip post (10), slip post (10) bottom with be connected with slewing mechanism between rotation post (19), the slip post (10) outside is connected with the dropping liquid device through fixed establishment.

2. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 1, which is characterized in that: the washing liquid box (14) with plummer (16) are the interval arrangement, washing liquid box (14) height is higher than plummer (16), be provided with the needle wash liquid in washing liquid box (14).

3. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 1, which is characterized in that: the top end of the inner wall of the bearing table (16) is provided with a groove matched with the NC film bearing plate (15), and one side of the top of the bearing table (16) and one side of the top far away from the rotating column (19) are of an open structure.

4. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 1, which is characterized in that: sliding column (10) outside is provided with a pair of outer beam (11), and is a pair of in outer beam (11) the bottom of one be provided with NC membrane loading board (15) complex dropping liquid cylinder (21), another outer beam (11) bottom be connected with through extending structure with lotion box (14) and plummer (16) complex electric heat stoving stick (18).

5. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 4, wherein: the upper end of the outer side of the electric heating drying rod (18) is movably sleeved with a limiting sleeve (12) fixed with the outer beam (11), and a spring (13) is connected between the top of the electric heating drying rod (18) and the top end of the inner part of the limiting sleeve (12).

6. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 4, wherein: the heating end of the electric heating drying rod (18) faces downwards, and a clamping plate (22) matched with the lotion box (14) is arranged at the position, above the heating end, of the outer side of the electric heating drying rod (18).

7. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 1, which is characterized in that: the bottom of the sliding column (10) is provided with a rotating sleeve (23) movably connected with the rotating column (19), the outer side of the rotating column (19) is provided with a plurality of groups of rotating sliding grooves (17), the inner wall of the rotating sleeve (23) is provided with a protrusion (20) matched with the rotating sliding grooves (17), and the rotating sliding grooves (17) are composed of an upper section vertical groove (1701), an ascending chute (1702), a descending chute (1703) and a lower section vertical groove (1704).

8. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 1, which is characterized in that: the glass cover (4) is arranged at the top of the fixing base (1), the top shell (24) is arranged at the top of the glass cover (4), the air filter (6) is arranged inside the top shell (24), and the operating panel is arranged on the outer side of the top shell (24).

9. The hybridization instrument for culturing the environmental-friendly aerobic bacteria according to claim 1, which is characterized in that: be connected with a pair of ventilating board (3) between glass dustcoat (4), vent (2) have been seted up to ventilating board (3) inner wall, ventilating board (3) with be connected with ventilation pipe (5) between air cleaner (6), air cleaner (6) with be connected with a pair of trachea of crossing between air pump dustcoat (8), cross the inside check valve that is provided with a pair of opposite direction of trachea, glass dustcoat (4) articulate has operation door (25).

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