Drawings
FIG. 1 is a schematic view of a first perspective in the present application;
FIG. 2 is a schematic view of a second perspective view of the present application;
FIG. 3 is a perspective view of a third perspective view of the present application;
FIG. 4 is a schematic view of the internal structure of the present application;
FIG. 5 is a schematic view of the operation platform according to the present application;
FIG. 6 is a top view of the operator's platform according to the present application;
FIG. 7 is a schematic diagram of a purification heating apparatus according to the present application;
FIG. 8 is a schematic diagram of a purification heating apparatus according to a second embodiment of the present application;
FIG. 9 is a schematic diagram of a purification heating apparatus according to the third embodiment of the present application;
FIG. 10 is a schematic view of a cartridge according to the present application;
FIG. 11 is a schematic perspective view of a pipetting assembly according to the application;
FIG. 12 is a schematic view of the structure of the X-direction moving assembly and the Y-direction moving assembly according to the present application;
fig. 13 is a schematic view showing the structure of the Z-direction moving component A, Z, the moving component B, Z, the moving component C, Z and the moving component D according to the present application;
FIG. 14 is a detailed view of the Z-direction moving assembly B according to the present application;
FIG. 15 is a schematic view of a Z-direction moving component D according to the present application;
FIG. 16 is a schematic view of the gun head and mounting box of the present application;
FIG. 17 is a schematic view of the connection structure of the gun head and the connector according to the present application;
FIG. 18 is a schematic view of a connector according to the present application;
FIG. 19 is a schematic view of the mounting structure of the connecting plate according to the present application;
FIG. 20 is a schematic view of a latch and spring according to the present application;
FIG. 21 is a schematic view showing the structure of a heat sealer according to the application;
FIG. 22 is a schematic view of a thermal seal plate according to the present application;
fig. 23 is a schematic structural view of a jacking device a according to the present application;
FIG. 24 is a schematic diagram of a jacking device A according to a second embodiment of the present application;
FIG. 25 is a schematic diagram showing the structure of a blocking device and a PCR plate recovery unit according to the present application;
FIG. 26 is a schematic view of a blocking device according to the present application;
FIG. 27 is a schematic view of a heat cover assembly according to the present application;
FIG. 28 is a second schematic structural view of the heat cover assembly of the present application;
FIG. 29 is a schematic view of the structure of the heat cover of the present application;
FIG. 30 is a schematic view of a metal bath module according to the present application;
FIG. 31 is a schematic view of the structure of a metal bath module and an optical detector according to the present application;
FIG. 32 is a schematic view of the position of the light filtering device according to the present application;
FIG. 33 is a schematic view of a moving member according to the present application;
FIG. 34 is a schematic diagram of a light filtering device according to the present application;
FIG. 35 is a schematic diagram of a second embodiment of an optical filter device according to the present application;
FIG. 36 is a schematic diagram of a third embodiment of an optical filter device according to the present application;
FIG. 37 is a schematic view of the optical path of the optical filter device of the present application.
In the accompanying drawings:
1-a housing, wherein the housing is provided with a plurality of grooves,
A 2-object placing window, wherein the object placing window is provided with a plurality of object placing windows,
A 3-viewing window for the light from the light source,
A 4-liquid path unit, wherein the liquid path unit comprises a liquid path unit,
A 5-power supply unit, which is connected with the power supply unit,
6-A recovery port A, wherein,
7-A recovery port B for recovering the residual oil,
8-Pipetting assembly, 81-X moving assembly, 811-X moving guide A,812-X moving guide B,813-X electric cylinder, 814-X chain; 82-Y direction moving component, 821-bearing plate, 8210-sub rail, 822-support A, 823-support B, 824-lead screw A,825-Y direction moving component, 826-X direction motor, 827-belt pulley A, 828-belt pulley B, 829-lead screw sleeve A;83-Z moving assembly A, 831-frame A, 8310-parent rail, 832-traverse A,833-Z motor A, 834-lead screw B, 835-lead screw sleeve B, 836-frame B, 84-Z moving assembly B,841-Z motor B, 842-traverse B, 843-guide rod, 844-lead screw C, 845-frame C, 846-lead screw sleeve C, 847-steel needle set A, 85-Z moving assembly C, 851-connecting piece, 852-housing, 853-Z motor C, 854-steel needle set B, 855-mounting plate, 856-high frequency valve, 857-tubing winder, 858-lead screw D, 859-lead screw sleeve D, 86-Z moving assembly D, 861-gun head, 862-mounting box, 8621-connecting rod, 8622-fixture block, 8623-spring A, 863-piston assembly, 864-piston assembly, 8641-connecting plate, 5-lead screw sleeve E, 866-E, 867-steel needle set B, 858-Z motor D, 868-frame D, 869-Z-frame D, 869-conical connector,
9-Operating platform, 91-gun head storage module, 92-sample cooling module, 93-reagent cooling module A, 94-needle cleaning module A,95-PCR plate, 96-needle cleaning module B, 97-reagent cooling module B, 98-positive reference cooling module, 99-gun head recovery component, 910-opening A, 911-opening B, 912-opening C,
10-Metal bath module, 101-heat cover assembly, 1011-stopper, 1012-through hole, 1013-metal heat cover, 1014-mounting bracket A, 1015-positioning hole, 1016-elastic member, 1018-positioning pin, 1019-moving member,
11-Optical detector, 111-optical filter, 1111-first filter, 1112-mounting bracket B, 1113-second filter, 1114-light receiving box, 1115-dichroic mirror, 1116-light passing hole, 1117-light, 1118-total reflection mirror,
12-Purification heating device, 1201-sample cartridge, 1202-heating groove, 1203-first heating chamber, 1204-second heating chamber, 1205-metal block, 1206-boss, 1207-placing table, 1208-through groove, 1209-heating plate, 1210-heating base,
13-Tray module, 131-tray a, 132-linear motor assembly a,
14-PCR plate transport module, 141-linear motor assembly B, 142-transport rack A,
15-Film sealing plate conveying modules, 151-linear motor assemblies C, 152-conveying frames B,
16-Heat sealing machine, 1601-tray B, 1602-sliding block, 1603-film sealing plate, 1604-heating plate, 1605-spring B, 1606-fixing plate, 1607-linear guide rail, 1608-cylinder, 1609-photoelectric sensor A, 1610-mounting base, 1611-synchronous belt driving mechanism, 1612-positioning port, 1613-lifting device A,
17-A separator plate, wherein the separator plate is provided with a plurality of grooves,
18-Blocking device, 181-blocking motor, 182-connecting block, 183-rotating wheel, 184-connecting long rod, 185-sliding groove, 186-baffle, 187-sliding groove module, 188-sliding rail module,
19-PCR board recovery component, 191-photoelectric sensor B, 192-plectrum.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides the following technical scheme:
as shown in fig. 4 and 5, the gene assaying device includes:
a purification heating device 12 for nucleic acid purification and extraction;
A pipetting assembly 8 for reagent, sample transfer;
an operation platform 9 for mounting reagents, samples, gun head processing modules;
a metal bath module 10 for PCR amplification reaction;
an optical detector 11 for gene detection;
A PCR plate transport module 14;
A film sealing plate conveying module 15;
A heat sealer 16 for PCR plate sealing film;
Wherein the purification heating device 12 is arranged at a first end of the operation platform 9, the metal bath module 10 and the optical detector 11 are arranged at a second end of the operation platform 9, the optical detector 11 is arranged at the upper part of the metal bath module 10, the reagent, the sample, the gun head processing module and the heat sealing machine 16 are arranged between the first end and the second end of the operation platform 9, the reagent, the sample and the gun head processing module are arranged near the first end of the operation platform 9, the heat sealing machine 16 is arranged near the second end of the operation platform 9, the pipetting assembly 8 is arranged at the upper part of the reagent, the sample, the gun head processing module and the purification heating device 12, the PCR plate conveying module 14 and the film sealing plate conveying module 15 are arranged on the operation platform 9 in parallel, the PCR plate conveying module 14 is used for conveying the reagent, the sample and the gun head processing module to the PCR plate 95 of the heat sealing machine, and the film sealing plate conveying module 15 is used for conveying the heat sealing machine 16 to the PCR plate 95 of the metal bath module 10.
In this embodiment, the PCR plate transporting module 14 includes a linear motor assembly B141 and a transporting frame a142, the moving end of the linear motor assembly B141 is connected to the transporting frame a142, the film sealing plate transporting module 15 includes a linear motor assembly C151 and a transporting frame B152, the moving end of the linear motor assembly C151 is connected to the transporting frame B152, and the moving range of the PCR plate transporting module is generally from the reagent, sample, and gun head processing module to the heat sealing machine, and the moving range of the film sealing plate transporting module is from the heat sealing machine to the PCR plate recycling assembly 19.
When the embodiment works, the working flow sequentially comprises the steps of purifying and amplifying sample nucleic acid by a purifying and heating device, transferring the sample to a PCR plate by a pipetting component 8, adding various reagents by the pipetting component 8, conveying the PCR plate to a heat sealing machine by a PCR plate conveying module for sealing the PCR plate, conveying the PCR plate to a metal bath module 10 for PCR amplification reaction after passing through a partition 17 by the sealing plate conveying module after sealing the PCR plate, and simultaneously carrying out optical detection by an optical detector 11, and conveying the detected PCR plate forward by the sealing plate conveying module for recovery by a PCR plate recovery component 19.
As shown in fig. 25, the PCR plate recycling assembly 19 includes a photoelectric sensor B191 and a paddle 192, when the PCR plate moves to the paddle 192, the paddle 192 contacts the PCR plate, and the paddle 192 pushes the PCR plate to drop into the opening C912 obliquely, and then is discharged from the recycling opening B.
As shown in fig. 5 and 6, the gene assaying device further comprises a tray module 13, wherein the tray module 13 comprises a tray a131 and a linear motor assembly a132, the linear motor assembly a132 is installed on the operation platform 9 and positioned between the reagent, the sample, the gun head processing module and the purifying and heating device, the tray a131 is connected with the moving part of the linear motor assembly a132, the tray a131 is synchronous with the lateral movement of the gun head 861 on the pipetting assembly 8, and the tray a131 is always arranged below the gun head 861 when the gun head 861 on the pipetting assembly 8 passes over the tray module 13.
In some embodiments, a microprocessor is provided to cooperatively control the whole device, and a control signal output end of the microprocessor is connected with a control signal input end of the moving assembly and a signal input end of the tray module.
As shown in fig. 11-15, the pipetting assembly 8 comprises:
An X-direction moving assembly 81 for moving the pipetting applying part in X-direction, wherein the X-direction moving assembly 81 comprises an X-direction moving guide A811, an X-direction moving guide B812 and an X-direction electric cylinder 813, the X-direction moving guide A811 and the X-direction moving guide B812 are parallel, and the Y-direction moving assembly 82 is fixedly arranged on a sliding part of the X-direction electric cylinder 813;
The Y-direction moving assembly 82 for enabling the pipetting action part to move along the Y direction comprises a bearing plate 821, a support A822, a support B823, a lead screw A824, a Y-direction moving part 825, an X-direction motor 826, a belt pulley A827, a belt pulley B828 and a lead screw sleeve A829, wherein the support A822 and the support B823 are respectively arranged at two ends of the bearing plate 821, the two ends of the lead screw A824 are rotatably arranged on the support A822 and the support B823, the lead screw sleeve A829 is arranged on the Y-direction moving part 825, the lead screw sleeve A829 is in threaded fit with the lead screw A824, the belt pulley B828 is fixedly connected with one end of the lead screw A824, the X-direction motor 826 is arranged on the bearing plate 821, a rotating shaft of the bearing plate 821 is fixedly connected with the belt pulley A827, and the belt pulley A827 is in transmission connection with the belt pulley B828;
The Z-direction moving assembly comprises a Z-direction moving assembly B84 for moving the steel needle group A847 along the Z direction, a Z-direction moving assembly C85 for moving the steel needle group B854 along the Z direction, a Z-direction moving assembly D86 for moving the gun head 861 along the Z direction, and a Z-direction moving assembly A83 for moving the Z-direction moving assembly B84, the Z-direction moving assembly C85 and the Z-direction moving assembly D86 along the Z direction;
wherein, Y is to the movable part of the movable part 81 of the X, and the movable part of the movable part 82 of the Y is to the movable part of the movable part 83 of the Z, and the movable parts of the Z, the movable parts of the Z84, the movable parts of the Z85 and the movable parts of the Z86 are all mounted on the movable part of the movable part A83 of the Z.
As shown in figures 11-20, the Z-direction moving assembly A83 comprises a frame A831, a transverse plate A832, a Z-direction motor A833, a screw rod B834, a screw rod sleeve B835 and a frame B826, wherein the frame A831 is fixedly arranged on the moving part of the Y-direction moving assembly 82, the transverse plate A832 is fixedly arranged in the frame A831, the Z-direction motor A833 is reversely arranged on the transverse plate A832, a rotating shaft of the Z-direction motor A833 passes through the transverse plate A832 to be fixedly connected with one end of the screw rod B834, the screw rod sleeve B835 is in threaded fit with the screw rod B834, the screw rod sleeve B835 is fixedly arranged on the frame B826, and the frame B826 can be vertically moved in the frame A831 and is positioned below the transverse plate A832;
The Z-direction moving assembly B84 comprises a Z-direction motor B841, a transverse plate B842, a guide rod 843, a screw rod C844, a frame C845, a screw rod sleeve C846 and a steel needle group A847, wherein the transverse plate B842 is fixedly arranged in the frame B826, the Z-direction motor B841 is reversely arranged on the transverse plate B842, a rotating shaft of the Z-direction motor B841 penetrates through the transverse plate B842 to be fixedly connected with one end of the screw rod C844, the screw rod sleeve C846 is in threaded fit with the screw rod C844, the screw rod sleeve C846 is fixedly arranged on the frame C845, the frame C845 can be arranged in the frame B826 in a vertically moving mode and is positioned below the transverse plate B842, the steel needle group A847 is arranged on the frame C845, and the steel needle group A847 is connected with an external liquid path;
The Z-direction moving assembly C85 comprises a connecting piece 851, a shell 852, a Z-direction motor C853, a steel needle group B854, a mounting plate 855, a high-frequency valve 856, a pipeline winder 857, a screw rod D858 and a screw rod sleeve D859, wherein the connecting piece 851 is connected with a frame B826, the Z-direction motor C853 is arranged on the connecting piece 851, the steel needle group B854, the high-frequency valve 856, the pipeline winder 857 and the screw rod sleeve D859 are all arranged on the mounting plate 855, the Z-direction motor C853 is vertically arranged in an inverted mode, the rotating shaft of the Z-direction motor C853 is fixedly connected with one end of the screw rod D858, the screw rod sleeve D859 is in threaded fit with the screw rod D858, an external liquid path is connected with one end of the high-frequency valve 856, and the other end of the high-frequency valve 856 is connected with the steel needle group B854 through the pipeline winder 857;
the Z-direction moving assembly D86 comprises a gun head 861, a mounting box 862, a connecting rod 8621, a clamping block 8622, a spring A8623, a piston cavity assembly 863, a connecting plate 8641, a screw rod sleeve E865, a screw rod E866, a frame D867, a Z-direction motor D868, a connector 869 and a conical pipe orifice 8691, wherein the frame D867 is fixedly connected with the frame B826, the Z-direction motor D868 is reversely arranged at the upper end of the frame D867, a rotating shaft of the Z-direction motor D868 penetrates through the upper end of the frame D867 and is fixedly connected with one end of the screw rod E866, the screw rod sleeve E865 is in threaded fit with the screw rod E866, the screw rod sleeve E865 is fixedly arranged at the upper end of a shell 852 of the piston assembly 864, the piston assembly 864 can move up and down and is arranged in the frame D867, the piston cavity assembly 863 is fixedly arranged in the frame D867, the piston cavity assembly 863 is arranged below the piston assembly 864, the piston cavity assembly 863 is in fit with the piston assembly 864, and the gun head 863 is arranged below the piston assembly 863 in fit with the piston assembly 863.
The Z-direction moving assembly D86 further comprises a mounting box 862, a connecting rod 8621, a clamping block 8622, a spring A8623, a connecting plate 8641 and a connector 869, wherein the connecting plate 8641 is arranged at the lower end of the piston assembly 864, a stepped through hole is vertically formed in the connecting plate 8641, the upper diameter of the stepped through hole is larger than the lower diameter, the upper end of the connecting rod 8621 is connected with the clamping block 8622, the lower end of the connecting rod 8621 is fixedly connected with the mounting box 862, the diameter of the clamping block 8622 is slightly smaller than that of the stepped through hole, the spring A8623 is sleeved on the connecting rod 8621, the spring A8623 is mounted between the step of the stepped through hole and the clamping block 8622, a plurality of through holes A are formed in the mounting box 862, the connector 869 is slidably mounted in the through hole A, a conical pipe orifice 8691 is arranged at the lower end of the connector 869, the conical pipe orifice 8691 is inserted into the upper end of the gun 861, when the piston assembly 864 is pressed down to the bottom, the clamping block 8622 is pressed down, the spring A8623 is compressed, the clamping block 8622 slides into the upper hole of the stepped through hole, and the connecting rod 8621 is pushed down to be separated from the mounting box 861.
In some embodiments, the number of the gun heads 861 is twelve, and the number of the gun heads 861 can be adjusted according to the number of rows and columns of the sample cartridges.
In some embodiments, the number of steel needles in steel needle set a847 is twelve, and the number of steel needles in steel needle set a847 may be adjusted according to the number of rows and columns of the sample cartridges.
In some embodiments, the number of steel needles of steel needle group B854 is typically four, in order to adapt to the shape of the reagent tank, although the number of steel needles of steel needle group B854 may be arbitrarily selected in order to adapt to different shapes of reagent tank.
In some embodiments, the gun head 861, the steel needle set A847 and the steel needle set B854 are all vertically and downwards arranged, the arrangement direction of the steel needles of the gun head 861 and the steel needle set A847 is parallel, and the arrangement direction of the steel needles of the steel needle set B854 is vertical.
In this embodiment, the movement of the Y-direction moving component 82 and the Z-direction moving component along the X-direction is realized by the operation of the X-direction cylinder 813, which is not described herein in the prior art.
When the embodiment works, the X-direction motor 826 provides main power, the power output by the X-direction motor 826 is transmitted to the screw rod A824 through the belt pulley A827, the screw rod A824 rotates, the Y-direction moving part 825 starts to walk, and the movement of the Z-direction moving assembly along the Y direction is realized.
In this embodiment, a through slot along the Y direction is provided on the carrying plate 821, and the Y-direction moving member 825 is engaged with the screw a824 through the screw sleeve a829 after passing through the through slot. Wherein the Y-direction moving member 825 is formed in a structure having a narrow upper end and a wide lower end in order to fit the through groove. Also, because the Y-direction moving member 825 is provided with an upward notch at its lower end for accommodating the Z-direction motor a833 in order to adapt to the installation of the Z-direction motor a833, the vertical space is saved.
In this embodiment, the pipetting assembly 8 further includes an X-direction drag chain 814 and a Y-direction drag chain, two ends of the X-direction drag chain 814 are respectively mounted on the X-direction moving rail a811 and the carrier plate 821 in a matching manner, two ends of the Y-direction drag chain are respectively mounted on a fixed peripheral device and the carrier plate 821 in a matching manner, and the drag chain is disposed in the prior art, so that the drag chain is used for binding cables and pipelines, and plays a role in dragging and protecting the cable pipelines.
In this embodiment, a mother rail 8310 is horizontally disposed on the frame a831, a child rail 8210 is horizontally disposed on the carrier plate 821, and the mother rail 8310 and the child rail 8210 are slidably engaged and are used to define the vertical displacement of the frame a 831. In other cases, two mother rails 8310 are respectively disposed in two sides of the frame a831, and two child rails 8210 are respectively disposed on two sides of the carrier plate 821, so as to maintain balance and facilitate relative sliding.
In some embodiments, the frame B826 and the frame D867 are integrally formed, protrusions are disposed on two sides of the integrally formed frame, corresponding to two sides of the frame a831, a chute is vertically formed, the width of the chute is slightly larger than that of the protrusions, the protrusions slide up and down along the chute, and the frame a831 limits the movement of the frame B826 and the frame D867 in the horizontal direction.
In some embodiments, the screw C844 may pass through an upper end of the frame C845 into an interior of the frame C845, with sufficient screw C844 movement space reserved inside the frame C845.
In some embodiments, the piston assembly 864 includes twelve pistons disposed side by side, the piston chamber assembly 863 includes twelve piston chambers disposed side by side, the pistons are mounted in cooperation with the piston chambers, the lower end of the screw rod E866 is connected with a push plate, the push plate is connected with the upper portions of the twelve pistons, and the screw rod E866 rotates in different directions to allow the pistons to advance or retract in the piston chambers, thereby completing reagent pumping.
In this embodiment, the through hole a formed in the mounting box 862 is directly smaller than the outer diameter of the gun 861, so that the gun 861 cannot enter the mounting box 862, and therefore the gun 861 can be pushed to be separated from the connector 869 when the mounting box 862 moves down.
In this embodiment, when another gun head 861 needs to be switched, the Z-direction motor D868 needs to be reversely operated, the screw rod E866 reversely rotates, the piston assembly 864 moves upward under the cooperation of the screw rod sleeve a829E, the spring a8623 rebounds to pull the connecting rod 8621 up, and then the mounting box 862 is pulled up, so that the conical nozzle 8691 of the connector 869 is exposed, and at this time, the new gun head 861 can be inserted through the conical nozzle 8691.
In some embodiments, the connecting member 851 is vertically mounted on a side wall of the frame B826, a protruding strip-shaped slider is vertically disposed on one side surface of the connecting member 851, a sliding groove is vertically disposed on one side of the mounting plate 855, and the slider is slidingly engaged with the sliding groove, and the movement of the mounting plate 855 in the horizontal direction is limited.
In some embodiments, the Z-direction moving assembly B84 further includes a guide rod 843, wherein a lower end of the guide rod 843 is fixedly mounted on an upper end of the frame C845, a guide groove is provided on the transverse plate B842, and the guide rod 843 is guided to fit through the guide groove on the transverse plate B842.
In some embodiments, the guide rods 843 are two in some embodiments and symmetrically distributed on two sides of the screw rod C844, the lower ends of the guide rods 843 are fixedly mounted on the upper ends of the frames C845, the upper ends of the guide rods 843 are mounted through guide grooves on the transverse plate B842, and the guide rods 843 are in sliding fit with the transverse plate B842.
In some embodiments, the pipetting assembly 8 comprises a code scanner 87, the code scanner 87 is mounted on a frame formed by integrating the frame B826 and the frame D867, and is used for scanning codes on the bar codes on the sample boxes, and only the sample boxes passing through the code scanner can cause the pipetting assembly 8 to work.
In the embodiment, three-direction pipetting actions are realized through an X-direction moving assembly 81, a Y-direction moving assembly 82 and a Z-direction moving assembly, primary regulation and control of a steel needle group A847, a steel needle group B854 and a gun head 861 in the Z direction are realized through a Z-direction moving assembly A83, secondary regulation and control of the steel needle group A847 in the Z direction is realized through a Z-direction moving assembly B84, secondary regulation and control of a steel needle group B854 in the Z direction is realized through a Z-direction moving assembly C85, secondary regulation and control of the gun head 861 in the Z direction is realized through a Z-direction moving assembly D86, stroke regulation and control of the steel needle group A847, the steel needle group B854 and the gun head 861 in the Z direction are enlarged, and in addition, the steel needle group A847, the steel needle group B854 and the gun head 861 are provided with different numbers and are suitable for transferring reagents in different reagent tanks and sample boxes.
As shown in fig. 5, the reagent, sample and gun head processing module comprises a gun head storage module 91 for storing gun heads 861, a sample cooling module 92, a reagent cooling module a93, a reagent cooling module B97, a needle cleaning module a94, a needle cleaning module B96, a positive reference object cooling module 98 and a gun head recovery assembly 99, wherein the gun head storage module 91, the sample cooling module 92, the reagent cooling module a93, the needle cleaning module a94, the needle cleaning module B96, the reagent cooling module B97, the positive reference object cooling module 98 and the gun head recovery assembly 99 are all arranged on the operation platform 9, the needle cleaning module a94 is used for cleaning a steel needle set a847, and the needle cleaning module B96 is used for cleaning a steel needle set B854.
In this embodiment, since the arrangement of the gun head 861 and the steel needle needs to be adapted, and the pipetting assembly 8 cannot rotate, the mounting orientations of the gun head storage module 91, the sample cooling module 92, the reagent cooling module a93, the needle cleaning module a94, the needle cleaning module B96, the reagent cooling module B97, the positive reference object cooling module 98 and the gun head recovery assembly 99 need to be determined according to the arrangement orientations of the gun head 861 and the steel needle, so that the gun head 861 and the steel needle can act in the corresponding processing module during working.
As shown in fig. 7-10, in some embodiments,
A purification heating apparatus 12 for heating a gene sample in a sample box 1201, comprising:
At least one first heating element, the heating tip of the first heating element acting on a gene sample having an identical heating requirement within the sample cell 1201;
A first heating chamber 1203, a first heating member mounted within the first heating chamber 1203;
The heating action end of the second heating element acts on all the gene samples in the sample box 1201, and the number of the gene samples acted by the heating action end of the first heating element is smaller than that of the gene samples acted by the heating action end of the second heating element;
a second heating chamber 1204, the second heating member being mounted within the second heating chamber 1204.
When it is necessary to heat all the gene samples in the sample box 1201, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201, and when only a certain part of the gene samples in the sample box 1201 need to be heated, the heating mode of the first heating element is selected to heat the gene samples in the sample box 1201, which need to be heated, without taking out the samples which need to be heated alone, and without affecting other gene samples which need not to be heated.
In some embodiments, the second heating element is a heating base 1210, a heating plate 1209 is installed inside the heating base 1210, and the upper surface of the heating base 1210 contacts the bottom of the sample box 1201 to heat all the gene samples in the sample box 1201.
When all the gene samples in the sample box 1201 need to be heated, the bottom of the sample box 1201 is placed on the upper surface of the heating base 1210, the upper surface of the heating base 1210 contacts with the bottom of the sample box 1201 to heat all the gene samples in the sample box 1201, and when only a certain part of the gene samples in the sample box 1201 need to be heated, the heating mode of the first heating element is selected to heat the gene samples of the sample box 1201 which need to be heated without independently taking out the samples which need to be heated, and meanwhile, other gene samples which do not need to be heated are not influenced.
In some embodiments, a plurality of heating grooves 1202 are formed on the upper surface of the heating base 1210, a boss 1206 is formed on the bottom of each sample position of the sample box 1201 in a protruding manner, and one heating groove 1202 is contacted with the boss 1206 on the bottom of one sample position to heat the gene sample in the sample position.
When all the gene samples in the sample box 1201 need to be heated, the boss 1206 at the bottom of each sample position of the sample box 1201 is contacted with the heating groove 1202 on the heating base 1210, so that the contact area between the bottom of the sample position and the heating base 1210 is increased, and the heating efficiency of the heating base 1210 on the gene samples is accelerated.
In some embodiments, the heating recess 1202 on the heating base 1210 and the boss 1206 at the bottom of the sample placement site are hemispherical.
Through with the heating recess 1202 on the heating base 1210 and put the boss 1206 of appearance position bottom and be hemispherical, further increase put appearance position bottom and the area of contact of heating base 1210, further accelerate the heating efficiency of heating base 1210 to the gene sample.
In some embodiments, the second heating element is a liquid and a heating plate 1209, the liquid is contained in the second heating chamber 1204, the heating plate 1209 is used to heat the liquid, the sample cell 1201 is positioned in the second heating chamber 1204 when heating the whole gene sample, the liquid wraps around the side and bottom of the sample cell 1201, the highest level of the liquid is slightly lower than the side wall height of the sample cell 1201, and the liquid is water.
When all the gene samples in the sample box 1201 need to be heated, the sample box 1201 is placed in the liquid in the second heating chamber 1204, so that the liquid wraps the side and bottom of the sample box 1201, the highest height of the liquid is slightly lower than the side wall height of the sample box 1201, the heating plate 1209 heats the liquid by heating the liquid, so that the gene samples in the sample box 1201 are subjected to heating treatment, and when only a certain part of the gene samples in the sample box 1201 need to be heated, the heating mode of the first heating element is selected to heat the gene samples which need to be heated in the sample box 1201 without independently taking out the samples which need to be heated, and meanwhile, other gene samples which do not need to be heated are not influenced.
In some embodiments, the first heating element comprises a metal block 1205, a heating rod is installed in the metal block 1205, the upper surface of the metal block 1205 is contacted with the bottom of a sample position to be heated in the sample box 1201 to heat the gene sample in the sample position, and the sample positions of the sample box 1201 are all rectangular and arrayed in the sample box 1201.
When all the gene samples in the sample box 1201 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201, the gene samples in the same row of sample positions are taken as one group, the conditions of all the processes are completely the same when the gene samples in the same group are subjected to experimental detection, the situation that the exception condition affects the final result is avoided, but certain conditions of the gene samples in different groups need to be different when the experimental detection is carried out, therefore, when the heating treatment is carried out, only one or more groups of gene samples in the sample box 1201 need to be heated, and when the heating mode of the first heating element is selected to heat the gene samples in the sample box 1201 need to be heated without taking the samples needing to be heated out singly, the sample box 1201 is placed in the first heating chamber 1203, the bottom of the sample position of the gene sample row needing to be heated is contacted with the upper surface of the metal block 1205, and the gene samples in the row of the sample position need to be heated are heated, and other gene samples needing not to be subjected to heating treatment are not affected.
In some embodiments, the upper surface of the metal block 1205 is provided with a plurality of heating grooves 1202, the bottom of each sample position of the sample box 1201 is outwards protruded to form a boss 1206, and one heating groove 1202 is contacted with the boss 1206 at the bottom of one sample position to heat the gene sample in the sample position.
The boss 1206 at the bottom of the row of sample positions of the sample box 1201 is contacted with the heating groove 1202 on the metal block 1205, so that the contact area between the boss 1206 at the bottom of the row of sample positions and the metal block 1205 is increased, and the heating efficiency of the metal block 1205 on the gene sample is accelerated.
In some embodiments, the heating recess 1202 on the metal block 1205 and the boss 1206 at the bottom of the sample placement site are hemispherical.
By arranging the heating groove 1202 on the metal block 1205 and the boss 1206 at the bottom of the sample placement position to be hemispherical, the contact area between the boss 1206 at the bottom of the sample placement position and the metal block 1205 is further increased, and the heating efficiency of the metal block 1205 on the gene sample is further accelerated.
In some embodiments, the first heating element comprises a placing table 1207 and a heat radiation infrared light source, at least one through groove 1208 is arranged on the placing table 1207, a sample placing position to be heated in the sample box is arranged at one end of the through groove 1208, the heat radiation infrared light source is arranged at the other end of the through groove 1208, and the heat radiation infrared light source passes through the through groove 1208 to heat the gene sample in the sample placing position.
When all the gene samples in the sample box 1201 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201, the gene samples in the same row of sample positions are taken as one group, the conditions of all the processes are completely the same when the gene samples in the same group are subjected to experimental detection, the situation that the exception condition affects the final result is avoided, but certain conditions of the gene samples in different groups need to be different when the experimental detection is performed, therefore, only one group or a plurality of groups of gene samples in the sample box 1201 need to be heated when the heating process is performed, when the samples needing to be heated are not taken out independently, the heating mode of the first heating element is selected to heat the gene samples in the sample box 1201, the sample box 1201 is placed in the first heating chamber 1203, the bottom of the sample position of the row of the gene samples needing to be heated is placed right above the through groove 1208 of the placing table 1207, the infrared light generated by the infrared light source is placed right below the through groove 1208 of the placing table, the infrared light source is parallel to the through groove 1208, and the infrared light source does not affect the heating of the gene samples, and the gene samples need to be heated when the other samples need to be heated.
As shown in fig. 21 to 24, the heat sealer 16 includes:
The film sealing plate 1603 is of a hollow structure, a plastic film for sealing the sample plate is fixed in the middle of the film sealing plate 1603, and at least two positioning ports 1612 are arranged on the film sealing plate 1603;
The hollow size of the film sealing plate 1603 is larger than the heat sealing surface size of the heating plate 1604, and the sample plate is positioned right below the heat sealing surface of the heating plate 1604;
a force application member for controlling the heating plate 1604 to be away from or close to the film sealing plate 1603;
And a reciprocating member 1019 for moving the film sealing plate 1603 to and from the heating plate 1604, wherein the moving direction of the film sealing plate 1603 is perpendicular to the moving direction of the heating plate 1604.
The force application member comprises a fixing plate 1606 and a cylinder 1608, wherein four through holes are formed in the fixing plate 1606 and the heating plate 1604, the heating plate 1604 and the fixing plate 1606 are connected through bolts, an elastic member 1016 is sleeved on a screw rod of each bolt, two ends of the elastic member 1016 are respectively in extrusion contact with opposite sides of the fixing plate 1606 and the heating plate 1604, a piston rod of the cylinder 1608 is fixedly connected with the fixing plate 1606, the reciprocating member 1019 comprises a tray B1601, two linear guide rails 1607, two sliding blocks 1602, a mounting base 1610 and a synchronous belt transmission mechanism 1611, a power output end of the synchronous belt transmission mechanism 1611 is fixedly connected with the tray B1601, the two sliding blocks 1602 are fixedly mounted on the mounting base 1610, one linear guide rail 1607 is slidably mounted on one sliding block 1602, the two linear guide rails 1607 are respectively fixed on two sides of the tray B1601, the sealing plate 1603 is placed on the tray B1601, and a photoelectric sensor A1609 for detecting whether the sealing plate 1603 is placed on the tray B1601 is arranged on the tray B1601.
The elastic member is a spring B1605.
The installation base 1610 is installed on the operation platform 9, an opening A is arranged on the operation platform 9 at the bottom of the installation base 1610, a jacking device A1613 is arranged on the operation platform 9 below the opening A, the conveying frame A of the PCR plate conveying module and the conveying frame B of the film sealing plate 1603 conveying module can pass through the installation base 1610 and the operation platform 9, the moving path of the conveying frame B is right above the moving path of the conveying frame A, and the jacking device A1613 is used for jacking the PCR plate positioned on the conveying frame A to the conveying frame B for placement.
The plastic film is fixed on the film sealing plate 1603 around, the plastic film is heated by the heating plate 1604 and downward acting force is applied, so that the plastic film is heat-sealed on the sample plate, the plastic film and the film sealing plate 1603 form a relatively fixed connection relationship, and the heat sealing surface of the heating plate 1604 is in non-contact with the outer frame of the film sealing plate 1603 in the heat sealing process because the hollow size of the film sealing plate 1603 is larger than the heat sealing surface size of the heating plate 1604, the outer frame of the film sealing plate 1603 can not generate thermal deformation, and the positioning accuracy of the subsequent procedures can not be affected because the positioning opening 1612 is arranged on the outer frame of the film sealing plate 1603 even if the sample plate is deformed by heating in the heat sealing process.
As shown in fig. 25 to 26, the gene assaying device comprises a blocking device 18, a partition 17 for preventing aerosol pollution is arranged on an operation platform 9 between the metal bath module 10 and the heat sealing machine, a through hole is formed in the partition 17, and the blocking device is used for controlling the opening and closing of the through hole.
The blocking device 18 comprises a blocking motor 181, a connecting block 182, a rotating wheel 183, a connecting long rod 184, a baffle 186, a sliding groove module 187 and a sliding rail module 188, wherein a penetrating sliding groove 185 is formed in the first end of the connecting long rod 184, the blocking motor 181 and the sliding rail module 188 are fixedly arranged on the operating platform 9, a rotating shaft of the blocking motor 181 is connected with one end of the connecting block 182, the other end of the connecting block 182 is connected with the rotating wheel 183, the rotating wheel 183 is slidably arranged in the sliding groove 185, the second end of the connecting long rod 184 is connected with the baffle 186, the sliding groove module 187 is fixedly arranged on the side wall of the second end of the connecting long rod 184, the sliding rail module 188 is in sliding fit with the sliding groove module 187, the direction of the sliding groove 185 is consistent with the length direction of the connecting long rod 184, the connecting long rod 184 is perpendicular to the sliding groove module 187 and the sliding rail module 188, and the baffle 186 is lifted or lowered when the blocking motor 181 rotates, and the baffle 186 is used for opening or blocking a through hole.
The blocking motor 181 in this embodiment is controlled by a microprocessor, when the PCR plate needs to pass through the partition 17, the blocking motor 181 is controlled to work, the rotating shaft of the blocking motor 181 drives the connecting block 182 to rotate, the connecting block 182 rotates to drive the rotating wheel 183 to roll along the sliding groove 185, the sliding groove module 187 slides upwards along the sliding rail module 188 to drive the baffle 186 to move upwards to expose the through hole on the partition 17, otherwise, the blocking motor 181 is controlled to rotate reversely, and the baffle 186 moves downwards to block the through hole on the partition 17.
As shown in fig. 27 to 31, the metal bath module 10 includes a heat cover assembly 101, the heat cover assembly 101 includes a metal heat cover 1013, and a plurality of through holes 1012 are provided in the metal heat cover 1013, and when the metal bath is performed, the center axis of one through hole 1012 coincides with the center axis of one sample position of the PCR plate.
The thermal cover assembly 101 further includes:
the positioning piece comprises at least two positioning pins 1018, the two positioning pins 1018 and the PCR plate are in a relative static state all the time, at least two positioning holes 1015 are arranged on the metal heat cover 1013, at least one positioning hole 1015 and other positioning holes 1015 are positioned on different sides of the metal heat cover 1013, and one positioning pin 1018 is inserted into one positioning hole 1015 during positioning.
The device comprises a mounting bracket A1014 and an elastic member 1016, wherein both ends of the elastic member 1016 are respectively connected with the mounting bracket A1014 and a metal heat cover 1013, and when the metal heat cover 1013 is contacted with a PCR plate, the elastic member 1016 is restored to deform in the same direction as the gravity direction.
The first end of each connecting rod 8621 is fixedly connected with the metal heat cover 1013, the mounting bracket A1014 is provided with a plurality of mounting holes, the elastic piece 1016 comprises a plurality of springs 8623, one connecting rod 8621 passes through one mounting hole, one spring 8623 is sleeved on the outer wall of one connecting rod 8621, and the section size of the mounting hole is larger than that of the connecting rod 8621.
The cross-sectional size of the limiting block 1011 is larger than that of the mounting hole, and the second end of the connecting rod 8621 is fixedly connected with the limiting block 1011.
The heat cover assembly 101 comprises a moving member 1019 installed at the lower part of the operation platform 9, an opening B is formed in the operation platform 9 corresponding to the upper part of the moving member 1019, the moving path of the conveying frame B passes through the bottom of the metal heat cover 1013, and the moving member 1019 moves upwards to jack the PCR plate on the conveying frame B of the film sealing plate conveying module to be connected with the metal heat cover 1013.
In this embodiment, the metal heat cover 1013 is provided with the plurality of through holes 1012, and the central axis of the through holes 1012 coincides with the central axis of the sample placement position of the reaction plate, so that when the reaction plate performs the PCR amplification reaction in the metal bath, the light pattern for performing fluorescent detection can reach the gene sample in the sample placement position, and the real-time detection of the gene sample in the PCR amplification reaction process can be realized, so that a more comprehensive detection result can be obtained.
The optical detector 11 includes a light filter 111, and the optical detector 11 is mounted on the upper part of the heat cover assembly 101 and acts on the PCR plate after passing through the through hole 1012;
As shown in fig. 32 to 37, the light filtering device 111 includes:
A dichroic mirror 1115 for reflecting the light source, the light source having an acute angle of incidence on the dichroic mirror 1115;
The light receiving box 1114 is used for absorbing the refracting light 1117 of the incident light 1117 on the dichroic mirror 1115, the refracting light 1117 is located on one side, away from the light receiving box 1114, of the normal, a light through hole 1116 is formed in the upper end of the light receiving box 1114, the dichroic mirror 1115 is arranged at the upper end of the light receiving box 1114, and the absorption end and the excitation light source of the light 1117 of the light receiving box 1114 are located on two sides of the dichroic mirror 1115.
The side wall and the bottom surface inside the light receiving box 1114 are both treated by black spraying.
When the light source passes through the dichroic mirror 1115, the light 1117 after refraction enters the light receiving box 1114 through refraction and reflection of the dichroic mirror 1115, and as the side wall and the bottom surface inside the light receiving box 1114 are subjected to black plastic spraying treatment, the refracted light 1117 can be absorbed, the light 1117 entering the light receiving box 1114 is prevented from being mixed with the light 1117 reflected by the dichroic mirror 1115 for the first time after being reflected by the light receiving box 1114 again, so that the accuracy of gene detection is reduced, the existence of stray light is reduced due to the absorption effect of the light 1117 of the light receiving box 1114, and the accuracy of gene detection is improved.
The angle between the bottom surface and the side wall of the interior of the light receiving box 1114 is not equal to 90 °.
The bottom surface slope setting inside the receipts light box 1114 will reach the light 1117 of receipts light box 1114 bottom surface after refracting again and reflect to the lateral wall of receipts light box 1114, increases the light absorption effect of receipts light box 1114, further improves the accuracy that detects.
The inside of the light receiving box 1114 is filled with black porous sponge. When the light source passes through the dichroic mirror 1115, the light 1117 after refraction enters the light receiving box 1114 through refraction and reflection of the dichroic mirror 1115, and as the side wall and the bottom surface inside the light receiving box 1114 are subjected to black plastic spraying treatment, the refracted light 1117 can be absorbed, the light 1117 entering the light receiving box 1114 is prevented from being mixed with the light 1117 reflected by the dichroic mirror 1115 for the first time after being reflected by the light receiving box 1114 again, so that the accuracy of gene detection is reduced, the existence of stray light is reduced due to the absorption effect of the light 1117 of the light receiving box 1114, and the accuracy of gene detection is improved.
The light filtering device 111 further includes:
A first optical filter 1111 for filtering other wavelengths of light, the first optical filter 1111 being fixedly mounted on the mounting bracket B1112, the first optical filter 1111 being located above the dichroic mirror 1115;
The upper end of the light receiving box 1114 is provided with a mounting hole, and the optical filter is arranged in the mounting hole of the light receiving box 1114;
Total reflection mirror 1118, and the reflection surface of total reflection mirror 1118 faces to dichroic mirror 1115, and the totally reflected light 1117 is parallel to the center axis of the PCR plate 95 to be inspected.
The initial emission light source filters the light 1117 with other wavelengths through the first optical filter 1111, the light 1117 reaching the dichroic mirror 1115 is reflected and refracted through the dichroic mirror 1115, the reflected light 1117 passes through the light through hole 1116 in the light receiving box 1114 and reaches the total reflection mirror 1118, the light 1117 reaching the PCR plate 95 after being reflected through the total reflection mirror 1118 and irradiates the gene sample in the PCR plate 95, the light 1117 reaching the PCR plate 95 is parallel to the central axis of the PCR plate 95, then the reflected light of the gene sample returns to the original path, the reflected light reaches the dichroic mirror 1115 after being reflected through the total reflection mirror 1118, the dichroic mirror 1115 is refracted and reflected again, and the incident direction of the light 1117 is located at one end of the normal line of the dichroic mirror 1115 close to the light receiving box 1114, so that the reflected light 1117 cannot be absorbed by the light receiving box 1114, even if only a small part of the reflected light is absorbed, and the refracted light 1117 can be collected through the second optical filter 1113 so as to be convenient for subsequent analysis.
The reflecting surface of the total reflecting mirror 1118 is parallel to the dichroic mirror 1115, and the included angle between the reflecting surface of the total reflecting mirror 1118 and the acute angle between the dichroic mirror 1115 and the gravity direction is 45 degrees, so that the reflected light 1117 of the total reflecting mirror 1118 is ensured to be parallel to the incident light 1117 of the dichroic mirror 1115, and therefore, the light 1117 of the initial emission light source is only required to face the central axis direction of the PCR plate 95, and the light 1117 reaching the PCR plate 95 can be ensured to be parallel to the central axis of the PCR plate 95.
The central axis of the second optical filter 1113 is perpendicular to the central axis of the light-receiving box 1114.
As shown in fig. 1,2 and 3, in some embodiments, the gene detection apparatus further includes a housing 1, a storage window 2, an observation window 3, a liquid path unit 4, a power supply unit 5, a recovery port A6 and a recovery port B7, wherein an operation platform 9 is installed in the lower portion in the housing 1, the liquid path unit 4 is installed in the back of the housing 1 and is connected to a liquid pipeline on a pipetting component 8, the power supply unit 5 is installed in the upper portion in the housing 1 and supplies power to all the apparatuses requiring power supply for the gene detection apparatus, the recovery port A6 and the recovery port B7 are both opened on the housing 1, the recovery port A6 is communicated with a gun head recovery component, the storage window 2 is opened at one end of the housing 1, a reagent is placed into a purification heating device through the storage window 2, the observation window 3 is opened at one side of the housing 1, and the operation of the pipetting component 8 and the operation of a heat seal machine can be observed through the observation window 3.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.