CN114164090A

Movatterモバイル変換

Info

Publication number: CN114164090A
Application number: CN202010950704.0A
Authority: CN
Inventors: 贾洪云; 李�杰; 何君; 邱浩; 何虎
Original assignee: Chengdu Hanchen Guangyi Bioengineering Co ltd
Current assignee: Chengdu Hanchen Guangyi Bioengineering Co ltd
Priority date: 2020-09-11
Filing date: 2020-09-11
Publication date: 2022-03-11
Anticipated expiration: 2040-09-11
Also published as: CN114164090B

Abstract

The invention discloses gene detection equipment, wherein a purification heating device is arranged at the first end of an operation platform, a metal bath module and an optical detector are arranged at the second end of the operation platform, the optical detector is arranged at the upper part of the metal bath module, a reagent, a sample, a gun head processing module and a heat sealing machine are arranged between the first end and the second end of the operation platform, a liquid transfer assembly is arranged at the upper parts of the reagent, the sample, the gun head processing module and the purification heating device, and a PCR plate conveying module and a membrane sealing plate conveying module are arranged on the operation platform in parallel; the PCR plate conveying module is used for conveying the reagent, the sample and the gun head processing module to a PCR plate of the heat sealing machine; the film sealing plate conveying module is used for conveying the PCR plate from the heat sealing machine to the metal bath module. Through purification heating device, move liquid subassembly, operation platform, metal bath module, optical detection appearance, PCR board transport module, envelope plate and transport the combined action of module, heat-sealing machine in this application, realized the automated inspection to the gene, increased detection efficiency.

Description

Gene detecting apparatus

Technical Field

The invention belongs to the technical field of gene detection, and particularly relates to gene detection equipment.

Background

The gene detection refers to the analysis of the base sequence of a specific DNA fragment, namely the arrangement mode of adenine, thymine, cytosine and guanine; the gene is a genetic basic unit, carries DNA or RNA sequence of genetic information, transmits the genetic information to the next generation through replication, and guides the synthesis of protein to express the genetic information carried by the gene, thereby controlling the character expression of organism individuals. The gene detection is a technique for detecting DNA by blood, other body fluids or cells, and is a method for taking peripheral venous blood or other tissue cells of a detected person, amplifying the gene information, detecting DNA molecular information in the cells of the detected person by a specific device, and analyzing whether the gene type, the gene defect and the expression function contained in the DNA molecular information are normal or not, so that people can know the gene information of themselves, determine the cause of disease or predict the risk of the body suffering from a certain disease, and the gene detection can diagnose the disease and can also be used for predicting the risk of the disease.

The gene detection equipment in the prior art cannot realize automation of the whole detection process, so that the gene detection efficiency is low.

Therefore, it is urgently required to develop a gene testing apparatus to solve the above problems.

Disclosure of Invention

To solve the problems set forth in the background art described above. The invention provides a gene detection device.

In order to achieve the purpose, the invention provides the following technical scheme:

a genetic testing device comprising:

a purification heating device for nucleic acid purification and extraction;

a pipetting assembly for reagent and sample transfer;

the operation platform is used for installing a reagent, a sample and a gun head processing module;

a metal bath module for PCR amplification reaction;

an optical detector for gene detection;

a PCR plate transport module;

a membrane sealing plate conveying module;

a heat sealer for sealing the PCR plate;

the device comprises a purification heating device, a metal bath module, an optical detector, a reagent, a sample, a gun head processing module and a heat sealing machine, wherein the purification heating device is arranged at the first end of an operation platform, the metal bath module and the optical detector are arranged at the second end of the operation platform, the optical detector is arranged at the upper part of the metal bath module, the reagent, the sample, the gun head processing module and the heat sealing machine are arranged between the first end and the second end of the operation platform, the reagent, the sample and the gun head processing module are arranged close to the first end of the operation platform, the heat sealing machine is arranged close to the second end of the operation platform, a liquid transfer assembly is arranged at the upper parts of the reagent, the sample, the gun head processing module and the purification heating device, and a PCR plate conveying module and a membrane sealing plate conveying module are arranged on the operation platform in parallel; the PCR plate conveying module is used for conveying the reagent, the sample and the gun head processing module to a PCR plate of the heat sealing machine; the film sealing plate conveying module is used for conveying the PCR plate from the heat sealing machine to the metal bath module.

Compared with the prior art, the invention has the beneficial effects that:

through purification heating device, move liquid subassembly, operation platform, metal bath module, optical detection appearance, PCR board transport module, envelope plate and transport the combined action of module, heat-sealing machine in this application, realized the automated inspection to the gene, increased detection efficiency.

Drawings

FIG. 1 is a perspective view of a first perspective in the present application;

FIG. 2 is a perspective view of a second perspective in the present application;

FIG. 3 is a perspective view of a third perspective in the present application;

FIG. 4 is a schematic diagram of the internal structure of the present application;

FIG. 5 is a schematic structural diagram of an operating platform of the present application;

FIG. 6 is a top view of the work platform of the present application;

FIG. 7 is a first schematic structural view of a purification heating apparatus according to the present application;

FIG. 8 is a schematic structural diagram II of a purification heating apparatus according to the present application;

FIG. 9 is a third schematic structural view of a purification heating apparatus of the present application;

FIG. 10 is a schematic view of the structure of a sample cartridge of the present application;

FIG. 11 is a perspective view of the pipetting assembly of the present application;

FIG. 12 is a schematic structural diagram of an X-direction moving assembly and a Y-direction moving assembly in the present application;

FIG. 13 is a schematic view of the structure of the Z-direction moving module A, Z moving toward moving module B, Z moving toward moving module C, Z moving toward moving module D;

FIG. 14 is a detailed view of the structure of the Z-direction moving assembly B of the present application;

FIG. 15 is a schematic structural view of a Z-direction moving assembly D of the present application;

FIG. 16 is a schematic structural diagram of a lance head and a mounting box in the present application;

FIG. 17 is a schematic view of a connection structure of the lance tip and the connector according to the present application;

FIG. 18 is a schematic view of the connector of the present application;

FIG. 19 is a schematic view of the mounting structure of the connection plate of the present application;

FIG. 20 is a schematic view of the structure of the latch and spring of the present application;

FIG. 21 is a schematic view of the construction of the heat sealer of the present application;

FIG. 22 is a schematic view of the construction of a heat seal plate according to the present application;

FIG. 23 is a first schematic structural view of a jacking device A of the present application;

fig. 24 is a second schematic structural view of the jacking device a in the present application;

FIG. 25 is a schematic view of the construction of the blocking device and PCR plate recovery unit of the present application;

FIG. 26 is a schematic view of the construction of the blocking device of the present application;

FIG. 27 is a first schematic view of a hot lid assembly according to the present application;

FIG. 28 is a second schematic structural view of a hot lid assembly of the present application;

FIG. 29 is a schematic view of the construction of a hot lid of the present application;

FIG. 30 is a schematic view of the structure of a metal bath module according to the present application;

FIG. 31 is a schematic view of the structure of a metal bath module and an optical inspection apparatus according to the present application;

FIG. 32 is a schematic view of the position of a light filtering device according to the present application;

FIG. 33 is a schematic view of a moving part of the present application;

FIG. 34 is a first schematic view of a light filtering device according to the present application;

FIG. 35 is a second schematic structural view of a light filtering device according to the present application;

FIG. 36 is a third schematic structural view of a light filtering device according to the present application;

FIG. 37 is a schematic illustration of the optical path of the optical filtering apparatus of the present application;

in the drawings:

1-a shell body, wherein the shell body is provided with a plurality of through holes,

2-an object placing window is arranged on the frame,

3-the observation window is arranged on the frame,

4-a liquid path unit, wherein,

5-a power supply unit, wherein,

6-the recovery port A is arranged on the upper part of the tower,

7-a recovery port B is arranged on the upper part of the tower,

8-pipetting assembly, 81-X direction moving assembly, 811-X direction moving guide rail A, 812-X direction moving guide rail B, 813-X direction electric cylinder and 814-X direction drag chain; 82-Y direction moving assembly, 821-bearing plate, 8210-sub rail, 822-support A, 823-support B, 824-screw rod A, 825-Y direction moving piece, 826-X direction motor, 827-belt pulley A, 828-belt pulley B, 829-screw rod sleeve A; 83-Z direction moving assemblies A, 831-frame A, 8310-mother rail, 832-transverse plate A, 833-Z direction motors A, 834-screw rod B, 835-screw rod sleeve B, 836-frame B; 84-Z direction moving assemblies B, 841-Z direction motors B, 842-transverse plates B, 843-guide rods, 844-screw rods C, 845-frames C, 846-screw rod sleeves C and 847-steel needle group A; 85-Z direction moving components C, 851-connecting pieces, 852-a housing, 853-Z direction motors C, 854-a steel needle group B, 855-a mounting plate, 856-a high-frequency valve, 857-a pipeline winder, 858-a lead screw D and 859-a lead screw sleeve D; 86-Z direction moving assemblies D, 861-gun heads, 862-mounting boxes, 8621-connecting rods, 8622-clamping blocks, 8623-springs A, 863-piston cavity assemblies, 864-piston assemblies, 8641-connecting plates, 865-screw rod sleeves E, 866-screw rods E, 867-frames D, 868-Z direction motors D, 869-connectors and 8691-tapered pipe orifices; 87-a code scanner which is arranged in the space,

9-operation platform, 91-gun head storage module, 92-sample cooling module, 93-reagent cooling module A, 94-needle cleaning module A, 95-PCR plate, 96-needle cleaning module B, 97-reagent cooling module B, 98-positive reference substance cooling module, 99-gun head recovery assembly, 910-opening A, 911-opening B, 912-opening C,

10-metal bath module, 101-hot cover component, 1011-limit component, 1012-through hole, 1013-metal hot cover, 1014-mounting support A, 1015-positioning hole, 1016-elastic component, 1018-positioning pin, 1019-moving component,

11-optical detector, 111-optical filter device, 1111-first optical filter, 1112-mounting support B, 1113-second optical filter, 1114-light-receiving box, 1115-two-way mirror, 1116-light-passing hole, 1117-light ray, 1118-total reflector,

12-purification heating device, 1201-sample box, 1202-heating groove, 1203-first heating chamber, 1204-second heating chamber, 1205-metal block, 1206-boss, 1207-placing table, 1208-through groove, 1209-heating plate, 1210-heating base,

13-tray module, 131-tray, 132-linear motor assembly a,

14-PCR plate transport module, 141-linear motor assembly B, 142-transport rack A,

15-a film sealing plate conveying module, 151-a linear motor assembly C, 152-a conveying frame B,

16-heat sealing machine, 1601-tray, 1602-slide block, 1603-sealing membrane plate, 1604-heating plate, 1605-spring B, 1606-fixing plate, 1607-linear guide rail, 1608-cylinder, 1609-photoelectric sensor A, 1610-mounting base, 1611-synchronous belt transmission mechanism, 1612-positioning port, 1613-jacking device A,

17-a partition plate which is arranged on the upper surface of the shell,

18-blocking device, 181-blocking motor, 182-connecting block, 183-rotating wheel, 184-connecting long rod, 185-sliding groove, 186-baffle, 187-sliding groove module, 188-sliding rail module,

19-PCR plate recovery component, 191-photoelectric sensor B, 192-plectrum.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides the following technical scheme:

as shown in fig. 4 and 5, the gene assaying device includes:

apurification heating device 12 for nucleic acid purification and extraction;

apipetting assembly 8 for reagent and sample transfer;

anoperation platform 9 for installing reagent, sample and gun head processing modules;

ametal bath module 10 for PCR amplification reaction;

anoptical detector 11 for gene detection;

a PCRplate conveyance module 14;

a membrane sealingplate conveyance module 15;

aheat sealer 16 for sealing the PCR plate;

thepurification heating device 12 is installed at the first end of theoperation platform 9, themetal bath module 10 and theoptical detector 11 are installed at the second end of theoperation platform 9, theoptical detector 11 is installed at the upper part of themetal bath module 10, the reagent, the sample, the gun head processing module and theheat sealing machine 16 are installed between the first end and the second end of theoperation platform 9, the reagent, the sample and the gun head processing module are installed close to the first end of theoperation platform 9, theheat sealing machine 16 is installed close to the second end of theoperation platform 9, theliquid transfer assembly 8 is installed at the upper parts of the reagent, the sample, the gun head processing module and thepurification heating device 12, and the PCRplate conveying module 14 and the membrane sealingplate conveying module 15 are installed on theoperation platform 9 in parallel; the PCRplate conveying module 14 is used for conveying the reagent, the sample and the gun head processing module to aPCR plate 95 of the heat sealing machine; the filmstrip transport module 15 is used for the transfer of theheat sealer 16 to thePCR strip 95 of themetal bath module 10.

In the present embodiment, the PCRplate transport module 14 includes a linear motor assembly B141, a transport rack a 142; the moving end of the linear motor component B141 is connected with the conveying frame A142; the film sealingplate conveying module 15 comprises a linear motor assembly C151 and a conveying frame B152; the moving end of the linear motor assembly C151 is connected to the conveying frame B152; the moving range of the PCR plate conveying module is generally from the reagent, sample and gun head processing module to the heat sealing machine, and the moving range of the membrane sealing plate conveying module is from the heat sealing machine to the PCRplate recovery assembly 19.

When the sample nucleic acid purification device works, the working procedures are sequentially that the sample nucleic acid is purified and extracted through a purification heating device; transfer the sample to the PCR plate by pipettingassembly 8; various reagents are added through thepipetting assembly 8, and various samples are operated; the PCR plate is conveyed to the heat sealing machine through the PCR plate conveying module to be subjected to PCR plate film sealing, the PCR plate is conveyed to themetal bath module 10 through the film sealing plate conveying module after being subjected to film sealing to pass through thepartition plate 17 to be subjected to PCR amplification reaction, optical detection is carried out through theoptical detector 11, and the PCR plate after detection is conveyed forward through the film sealing plate conveying module and is recycled through the PCRplate recycling assembly 19.

As shown in FIG. 25, the PCRplate recycling assembly 19 comprises a photoelectric sensor B191 and apaddle 192, when the PCR plate moves to thepaddle 192, thepaddle 192 contacts with the PCR plate, and thepaddle 192 tilts the PCR plate to fall into the opening C912 and then is discharged from the recycling opening B.

As shown in fig. 5 and 6, the gene assaying device further includes: tray module, tray module includes tray and linear electric motor subassembly A, linear electric motor subassembly A installs onoperation platform 9 to be located reagent, sample, between rifle head processing module and the purification heating device, the tray is connected with linear electric motor subassembly A's removal part, the tray is synchronous with the lateral shifting ofrifle head 861 on pipettingassembly 8, whenrifle head 861 on pipettingassembly 8 passes through tray module top,rifle head 861 below is arranged in all the time to the tray.

In some embodiments, a microprocessor is provided to cooperatively control the entire apparatus, and a control signal output terminal of the microprocessor is connected to a control signal input terminal of the moving assembly and a signal input terminal of the tray module.

As shown in fig. 11-15, pipettingassembly 8 comprises:

anX-direction moving unit 81 for moving the pipetting unit in the X-direction; theX-direction moving unit 81 includes an X-direction moving guide a811, an X-direction moving guide B812, and an X-directionelectric cylinder 813; the X-direction moving guide a811 is parallel to the X-direction moving guide B812; the Y-direction moving assembly 82 is fixedly arranged on the sliding part of the X-directionelectric cylinder 813;

a Y-direction moving unit 82 for moving the pipetting unit in the Y-direction; the Y-direction moving assembly 82 comprises abearing plate 821, a support A822, a support B823, a screw A824, a Y-direction moving piece 825, anX-direction motor 826, a belt pulley A827, a belt pulley B828 and a screw sleeve A829; the support A822 and the support B823 are respectively installed at two ends of thebearing plate 821, and two ends of the screw rod A824 are rotatably installed on the support A822 and the support B823; the screw rod sleeve A829 is installed on the Y-direction walking piece 825, the screw rod sleeve A829 is installed with the screw rod A824 in a threaded fit mode, the belt pulley B828 is fixedly connected with one end of the screw rod A824, theX-direction motor 826 is installed on thebearing plate 821, the rotating shaft of thebearing plate 821 is fixedly connected with the belt pulley A827, and the belt pulley A827 is in transmission connection with the belt pulley B828;

a Z-direction moving unit for moving the pipetting unit in the Z-direction; the Z-direction moving assembly comprises a Z-direction moving assembly B84 for moving the steel needle group A847 along the Z direction, a Z-direction moving assembly C85 for moving the steel needle group B854 along the Z direction, a Z-direction moving assembly D86 for moving thegun head 861 along the Z direction, and a Z-direction moving assembly A83 for moving the Z-direction moving assembly B84, the Z-direction moving assembly C85 and the Z-direction moving assembly D86 along the Z direction;

wherein, the Y-direction moving assembly 82 is arranged on the moving part of theX-direction moving assembly 81, the Z-direction moving assembly A83 is arranged on the moving part of the Y-direction moving assembly 82, and the Z-direction moving assembly B84, the Z-direction moving assembly C85 and the Z-direction moving assembly D86 are all arranged on the moving part of the Z-direction moving assembly A83.

As shown in fig. 11-20, the Z-direction moving assembly a83 includes a frame a831, a horizontal plate a832, a Z-direction motor a833, a lead screw B834, a lead screw sleeve B835 and a frame B826; the frame A831 is fixedly installed on the moving part of the Y-direction moving assembly 82, the transverse plate A832 is fixedly installed in the frame A831, the Z-direction motor A833 is installed on the transverse plate A832 in an inverted mode, the rotating shaft of the Z-direction motor A833 penetrates through the transverse plate A832 and is fixedly connected with one end of the lead screw B834, the lead screw sleeve B835 is installed with the lead screw B834 in a threaded fit mode, and the lead screw sleeve B835 is fixedly installed on the frame B826; the frame B826 can be vertically moved and is arranged in the frame A831 and is positioned below the transverse plate A832;

the Z-direction moving assembly B84 comprises a Z-direction motor B841, a transverse plate B842, aguide rod 843, a screw rod C844, a frame C845, a screw rod sleeve C846 and a steel needle group A847, wherein the transverse plate B842 is fixedly installed in the frame B826, the Z-direction motor B841 is inversely installed on the transverse plate B842, a rotating shaft of the Z-direction motor B841 penetrates through the transverse plate B842 to be fixedly connected with one end of the screw rod C844, the screw rod sleeve C846 is installed in threaded fit with the screw rod C844, and the screw rod sleeve C846 is fixedly installed on the frame C845; the frame C845 can be vertically moved and placed in the frame B826 and is positioned below the transverse plate B842, the steel needle group A847 is installed on the frame C845, and the steel needle group A847 is connected with an external liquid path;

the Z-direction moving assembly C85 comprises a connectingpiece 851, ashell 852, a Z-direction motor C853, a steel needle group B854, a mountingplate 855, a high-frequency valve 856, apipeline winder 857, a lead screw D858 and a lead screw sleeve D859, wherein the connectingpiece 851 is connected with a frame B826, the Z-direction motor C853 is installed on the connectingpiece 851, the steel needle group B854, the high-frequency valve 856, thepipeline winder 857 and the lead screw sleeve D859 are all installed on the mountingplate 855, the Z-direction motor C853 is installed in an inverted vertical mode, the rotating shaft of the Z-direction motor C853 is fixedly connected with one end of the lead screw D858, the lead screw sleeve D859 is installed in a threaded fit mode with the lead screw D858, an external liquid path is connected with one end of the high-frequency valve 856, and the other end of the high-frequency valve 856 is connected with the steel needle group B854 through thepipeline winder 857;

the Z-direction moving assembly D86 comprises a gun head 861, a mounting box 862, a connecting rod 8621, a clamping block 8622, a spring A8623, a piston cavity assembly 863, a piston assembly 864, a connecting plate 8641, a screw rod sleeve E865, a screw rod E866, a frame D867, a Z-direction motor D868, a connector 869 and a conical nozzle 8691; the frame D867 is fixedly connected with the frame B826, the Z-direction motor D868 is installed at the upper end of the frame D867 in an inverted mode, a rotating shaft of the Z-direction motor D868 penetrates through the upper end of the frame D867 to be fixedly connected with one end of the screw rod E866, the screw rod sleeve E865 is installed with the screw rod E866 in a threaded fit mode, the screw rod sleeve E865 is fixedly installed at the upper end of a shell 852 of the piston assembly 864, and the piston assembly 864 is installed in the frame D867 in a vertically movable mode; the piston cavity assembly 863 is fixedly arranged in the frame D867, the piston cavity assembly 863 is arranged below the piston assembly 864, and the piston cavity assembly 863 and the piston assembly 864 are arranged in a matching way; gun head 861 is positioned below piston chamber assembly 863 and is in communicative engagement with piston chamber assembly 863.

The Z-direction moving assembly D86 further comprises a mounting box 862, a connecting rod 8621, a clamping block 8622, a spring A8623, a connecting plate 8641 and a connector 869; the connecting plate 8641 is arranged at the lower end of the piston assembly 864, a stepped through hole is vertically formed in the connecting plate 8641, the diameter of the upper part of the stepped through hole is larger than that of the lower part of the stepped through hole, the upper end of the connecting rod 8621 is connected with the fixture block 8622, the lower end of the connecting rod 8621 is fixedly connected with the mounting box 862, the diameter of the fixture block 8622 is slightly smaller than that of the stepped through hole, the spring A8623 is sleeved on the connecting rod 8621, and the spring A8623 is mounted between the step of the stepped through hole and the fixture block 8622; the mounting box 862 is provided with a plurality of through holes a, a plurality of connectors 869 are slidably mounted in the through holes a, the lower end of the connector 869 is provided with a tapered pipe orifice 8691, and the tapered pipe orifice 8691 is inserted into the upper end of the gun head 861 for mounting; when the piston assembly 864 is pressed downwards to the bottom, the piston assembly 864 presses the block 8622 downwards, the spring a8623 is compressed, the block 8622 slides into the upper hole of the stepped through hole, the connecting rod 8621 pushes the mounting box 862 downwards, and the mounting box 862 pushes the gun head 861 to be separated from the tapered nozzle 8691.

In some embodiments, the number oftips 861 is twelve, and the number oftips 861 may be adjusted according to the number of rows and columns of the sample box.

In some embodiments, the number of steel pins in the steel pin group a847 is twelve, and the number of steel pins in the steel pin group a847 can be adjusted according to the number of rows and columns of the sample box.

In some embodiments, the number of the steel pins of the steel pin set B854 is generally four, so as to be adapted to the shape of the reagent tank, but the number of the steel pins of the reagent channel steel pin set B854 can be arbitrarily selected so as to be adapted to different shapes.

In some embodiments, the gun heads 861, the steel needle group A847 and the steel needle group B854 are all vertically arranged downwards; the arrangement directions of the gun heads 861 and the steel needles of the steel needle group A847 are parallel, and the arrangement directions of the uniform heads of the steel needle group B854 and the steel needles of the steel needle group A847 are vertical.

In this embodiment, the Y-direction moving unit 82 and the Z-direction moving unit move in the X direction by the operation of the X-directionelectric cylinder 813, which is not described in the related art.

When the X-direction moving assembly works, theX-direction motor 826 provides main power, the power output by theX-direction motor 826 is transmitted to the screw rod A824 through the belt pulley A827, the screw rod A824 rotates, the Y-direction moving member 825 starts to move, and the Z-direction moving assembly moves along the Y direction.

In this embodiment, a through slot along the Y direction is provided on thebearing plate 821, and the Y-direction moving member 825 passes through the through slot and then is engaged with the lead screw a824 through the lead screw sleeve a 829. The Y-direction running member 825 is formed to have a narrow upper end and a wide lower end so as to fit the through groove. And because the Y-direction walking piece 825 is matched with the Z-direction motor A833, the lower end of the Y-direction walking piece is provided with an upward notch for placing the Z-direction motor A833, so that the vertical space is saved.

In this embodiment, thepipetting assembly 8 further includes anX-direction drag chain 814 and a Y-direction drag chain, and two ends of theX-direction drag chain 814 are respectively installed on the X-direction moving guide rail a811 and thebearing plate 821 in a matching manner; two ends of the Y-direction drag chain are respectively installed on a fixed peripheral device and thebearing plate 821 in a matching way; the arrangement of tow chain belongs to prior art, and the tow chain is in order to tie cable and pipeline, plays the effect of drawing and protection cable duct.

In this embodiment, the frame a831 is horizontally provided with amain rail 8310, theloading plate 821 is horizontally provided with asub rail 8210, and themain rail 8310 is in sliding fit with thesub rail 8210 and is used for limiting the vertical displacement of the frame a 831. In other cases, the twomain rails 8310 are respectively disposed in the two sides of the frame a831, and the twosub rails 8210 are respectively disposed in the two sides of thebearing plate 821, so as to keep balance and facilitate relative sliding.

In some embodiments, the frames B826 and D867 are integrally formed, the protrusions are disposed on two sides of the integrally formed frames, the sliding grooves are vertically formed on two sides of the frame a831, the width of the sliding grooves is slightly larger than that of the protrusions, the protrusions slide up and down along the sliding grooves, and the frame a831 limits the horizontal movement of the frames B826 and D867.

In some embodiments, the screw C844 may pass through the upper end of the frame C845 into the inside of the frame C845, and enough space for the screw C844 to move is reserved inside the frame C845.

In some embodiments, twelve pistons are disposed side by side in thepiston assembly 864, twelve piston chambers are disposed side by side in thepiston chamber assembly 863, the pistons are mounted in cooperation with the piston chambers, a push plate is connected to a lower end of the screw rod E866, the push plate is connected to upper portions of the twelve pistons, and the screw rod E866 rotates in different directions to allow the pistons to advance or retreat in the piston chambers to complete aspiration and spit of samples.

In this embodiment, the through hole a formed in themounting box 862 is directly smaller than the outer diameter of thelance tip 861, so that thelance tip 861 cannot enter themounting box 862, and therefore thelance tip 861 can be pushed away from theconnector 869 when the mountingbox 862 moves downward.

In this embodiment, when anothergun head 861 needs to be switched again, the Z-direction motor D868 needs to work reversely, the screw rod E866 rotates reversely, thepiston assembly 864 moves upwards under the matching action of the screw rod sleeve a829E, the spring a8623 rebounds to pull up the connectingrod 8621, and further pulls up the mountingbox 862, so that the taperednozzle 8691 of theconnector 869 is exposed, and at this time, anew gun head 861 can be inserted into the taperednozzle 8691.

In some embodiments, the connectingmember 851 is vertically mounted on the side wall of the frame B826, a raised strip-shaped slider is vertically disposed on one side of the connectingmember 851, a sliding slot is vertically disposed on one side of the mountingplate 855, and the slider and the sliding slot are slidably engaged and limit the horizontal movement of the mountingplate 855.

In some embodiments, the Z-direction moving assembly B84 further includes aguide rod 843, the lower end of theguide rod 843 is fixedly mounted on the upper end of the frame C845, a guide slot is formed on the cross plate B842, and theguide rod 843 is guided and matched through the guide slot on the cross plate B842.

In some embodiments, twoguide rods 843 are symmetrically distributed on two sides of the screw rod C844 in some embodiments, lower ends of the twoguide rods 843 are fixedly mounted at an upper end of the frame C845, upper ends of the twoguide rods 843 are mounted through guide grooves in the transverse plate B842, and theguide rods 843 are in sliding fit with the transverse plate B842.

In some embodiments, thepipetting assembly 8 includes ascanner 87, thescanner 87 is mounted on a frame integrally formed with frame B826 and frame D867 and is used to scan a barcode on a sample cartridge; only the sample cartridge that is scanned past will cause thepipetting assembly 8 to operate.

In the present embodiment, the pipetting motions in three directions are realized by theX-direction moving unit 81, the Y-direction moving unit 82, and the Z-direction moving unit; the Z-direction moving assembly A83 is used for realizing the primary regulation and control of the steel needle group A847, the steel needle group B854 and thegun head 861 in the Z direction; the secondary regulation of the steel needle group A847 in the Z direction is realized through the Z-direction moving assembly B84, and the secondary regulation of the steel needle group B854 in the Z direction is realized through the Z-direction moving assembly C85; the secondary regulation and control of thegun head 861 in the Z direction are realized through the Z-direction moving assembly D86, and the stroke regulation and control of the steel needle group A847, the steel needle group B854 and thegun head 861 in the Z direction are enlarged; in addition, the steel needle group A847, the steel needle group B854 and the gun heads 861 are different in number and are suitable for transferring reagents in different reagent tanks and sample boxes.

As shown in fig. 5, the reagent, sample and lance head processing module includes a lancehead storage module 91, asample cooling module 92, a reagent cooling module a93, a reagent cooling module B97, a needle cleaning module a94, a needle cleaning module B96, a positivereference cooling module 98 and a lancehead recovery assembly 99 for storing thelance head 861, and the lancehead storage module 91, thesample cooling module 92, the reagent cooling module a93, the needle cleaning module a94, the needle cleaning module B96, the reagent cooling module B97, the positivereference cooling module 98 and the lancehead recovery assembly 99 are all mounted on theoperation platform 9; the needle cleaning module A94 is used for cleaning the steel needle group A847, and the needle cleaning module B96 is used for cleaning the steel needle group B854.

In this embodiment, since the arrangement of the gun heads 861 and the steel needles needs to be adapted, and thepipetting assembly 8 cannot rotate, the installation orientations of the gunhead storage module 91, thesample cooling module 92, the reagent cooling module a93, the needle head cleaning module a94, the needle head cleaning module B96, the reagent cooling module B97, the positive referencesubstance cooling module 98 and the gunhead recovery assembly 99 need to be determined according to the arrangement orientations of the gun heads 861 and the steel needles, so as to ensure that the gun heads 861 and the steel needles can act in the corresponding processing modules during operation.

As shown in fig. 7-10, in some embodiments,

apurification heating device 12 for heating the gene sample in thesample cell 1201, comprising:

at least one first heating element; the heating action end of the first heating element acts on the gene sample with the same heating requirement in thesample box 1201;

afirst heating chamber 1203; the first heating member is installed in thefirst heating chamber 1203;

a second heating member; the heating action ends of the second heating elements act on all the gene samples in thesample box 1201, and the number of the gene samples acted on by the heating action ends of the first heating elements is less than that of the gene samples acted on by the heating action ends of the second heating elements;

asecond heating chamber 1204; the second heating element is installed in thesecond heating chamber 1204.

When all the gene samples in thesample box 1201 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in thesample box 1201, and when only a certain part of the gene samples in thesample box 1201 need to be heated, the heating mode of the first heating element is selected to heat the gene samples needing to be heated in thesample box 1201 without independently taking out the samples needing to be heated, and meanwhile, other gene samples which do not need to be heated are not affected.

In some embodiments: the second heating element is aheating base 1210, aheating sheet 1209 is installed inside theheating base 1210, and the upper surface of theheating base 1210 contacts with the bottom of thesample box 1201 to heat all the gene samples in thesample box 1201.

When all the gene samples in thesample box 1201 need to be heated, the bottom of thesample box 1201 is placed on the upper surface of theheating base 1210, and the upper surface of theheating base 1210 is in contact with the bottom of thesample box 1201 to heat all the gene samples in thesample box 1201; when only a certain part of the gene sample in thesample box 1201 needs to be heated, the gene sample to be heated in thesample box 1201 is heated by selecting the heating mode of the first heating member without taking out the sample to be heated separately, and other gene samples which do not need to be heated are not affected.

In some embodiments: the upper surface of theheating base 1210 is provided with a plurality ofheating grooves 1202, the bottom of each sample placement position of thesample box 1201 protrudes outwards to form aboss 1206, and oneheating groove 1202 contacts with theboss 1206 at the bottom of one sample placement position to heat the gene sample in the sample placement position.

When all the gene samples in thesample box 1201 need to be heated, theboss 1206 at the bottom of each sample placement position of thesample box 1201 is in contact with theheating groove 1202 on theheating base 1210, so that the contact area between the bottom of the sample placement position and theheating base 1210 is increased, and the heating efficiency of theheating base 1210 on the gene samples is accelerated.

In some embodiments: theheating groove 1202 on theheating base 1210 and theboss 1206 at the bottom of the sample placement position are both hemispherical.

Theheating groove 1202 on theheating base 1210 and theboss 1206 at the bottom of the sample placement position are both hemispherical, so that the contact area between the bottom of the sample placement position and theheating base 1210 is further increased, and the heating efficiency of theheating base 1210 on the gene sample is further accelerated.

In some embodiments, the second heating element is a liquid and aheating sheet 1209, the liquid is contained in thesecond heating chamber 1204, theheating sheet 1209 is used for heating the liquid, when the whole gene sample is heated, thesample box 1201 is positioned in thesecond heating chamber 1204, the liquid is wrapped at the side and the bottom of thesample box 1201, the highest height of the liquid is slightly lower than the height of the side wall of thesample box 1201, and the liquid is water.

When all the gene samples in thesample box 1201 need to be heated, thesample box 1201 is placed in the liquid in thesecond heating chamber 1204, so that the liquid is wrapped at the side and the bottom of thesample box 1201, the highest height of the liquid is slightly lower than the height of the side wall of thesample box 1201, and theheating sheet 1209 heats the liquid by heating the liquid, so that the gene samples in thesample box 1201 are subjected to heating treatment; when only a certain part of the gene sample in thesample box 1201 needs to be heated, the gene sample to be heated in thesample box 1201 is heated by selecting the heating mode of the first heating member without taking out the sample to be heated separately, and other gene samples which do not need to be heated are not affected.

In some embodiments: the first heating member comprises ametal block 1205, a heating rod is installed in themetal block 1205, the upper surface of themetal block 1205 is in contact with the bottom of a sample placement position needing to be heated in thesample box 1201 to heat the gene sample in the sample placement position, and the sample placement positions of thesample box 1201 are all in a rectangular array in thesample box 1201.

When all the gene samples in thesample box 1201 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in thesample box 1201; the gene samples in the same row of sample positions are used as a group, the conditions of all the processes when the gene samples in the same group are tested are completely the same, the influence of the exceptional conditions on the final result is avoided, however, since some conditions of different groups of gene samples need to be different during experimental detection, when heat treatment is performed, it may be necessary to heat only one or more groups of gene samples in thesample cassette 1201, when the heating pattern of the first heating member is selected to heat-treat the gene sample to be heated in thesample cartridge 1201 without separately taking out the sample to be heat-treated, thesample box 1201 is placed in thefirst heating chamber 1203, the bottoms of the sample placement positions of the row of gene samples needing to be heated are in contact with the upper surface of themetal block 1205, so that the gene samples in the row of sample placement positions are heated, and meanwhile, other gene samples which do not need to be heated are not affected.

In some embodiments: the upper surface of themetal block 1205 is provided with a plurality ofheating grooves 1202, the bottom of each sample placement position of thesample box 1201 protrudes outwards to form aboss 1206, and oneheating groove 1202 contacts with theboss 1206 at the bottom of one sample placement position to heat the gene sample in the sample placement position.

Theboss 1206 at the bottom of the sample placement position of thesample box 1201 is in contact with theheating groove 1202 on themetal block 1205, so that the contact area between theboss 1206 at the bottom of the sample placement position of the line and themetal block 1205 is increased, and the heating efficiency of themetal block 1205 on the gene sample is accelerated.

In some embodiments: theheating groove 1202 on themetal block 1205 and theboss 1206 at the bottom of the sample placement position are both hemispherical.

Theheating groove 1202 on themetal block 1205 and theboss 1206 at the bottom of the sample placement position are both hemispherical, so that the contact area between theboss 1206 at the bottom of the sample placement position and themetal block 1205 is further increased, and the heating efficiency of themetal block 1205 on the gene sample is further accelerated.

In some embodiments: first heating member is includingplacing platform 1207 and thermal radiation infrared light source, places and sets up at least onelogical groove 1208 on theplatform 1207, and the appearance position of putting that needs the heating in the sample box is placed in the one end that leads to groove 1208, and the other end that leads to groove 1208 is placed in to thermal radiation infrared light source, and thermal radiation infrared light source passes throughlogical groove 1208 and heats this gene sample who puts in the appearance position.

When all the gene samples in the sample box 1201 need to be heated, the heating mode of the second heating element is selected to heat all the gene samples in the sample box 1201; the gene samples in the same row of sample placement positions are used as a group, conditions of all processes when the gene samples in the same group are subjected to experimental detection are completely the same, the influence of an exceptional condition on a final result is avoided, but certain conditions of the gene samples in different groups need to be different when the experimental detection is carried out, so that only one group or a plurality of groups of gene samples in the sample box 1201 need to be subjected to heating treatment when the heating treatment is carried out, under the condition that the samples needing to be subjected to the heating treatment are not taken out independently, the sample box 1201 is placed in the first heating chamber 1203 when the heating mode of the first heating element is selected to carry out the heating treatment on the gene samples needing to be heated in the sample box 1201, the bottoms of the sample placement positions of the row of gene samples needing to be heated are placed right above the through grooves 1208 of the placing table 1207, the thermal radiation infrared light source is placed right below the through grooves 1208 of the placing table 1207, the infrared light that thermal radiation infrared light source produced is parallel with logical groove 1208, and thermal radiation infrared light source passes and leads to groove 1208 to heat this row of gene sample of putting in the appearance position, also can not influence other gene samples that need not carry out heat treatment simultaneously.

As shown in fig. 21 to 24, theheat sealer 16 includes:

afilm sealing plate 1603; themembrane sealing plate 1603 is of a hollow structure, a plastic membrane for membrane sealing of the sample plate is fixed in the middle of themembrane sealing plate 1603, and at least twopositioning holes 1612 are formed in themembrane sealing plate 1603;

aheating plate 1604 for heating the plastic film; the hollow size offilm sealing plate 1603 is larger than the heat sealing surface size ofheating plate 1604, and the sample plate is positioned right below the heat sealing surface ofheating plate 1604;

a force applying member for controlling theheating plate 1604 to move away from or close to thesealing plate 1603;

ashuttle 1019 for moving thesealing plate 1603 to between theheating plate 1604 and the sample plate; the direction of movement of thesealing plate 1603 is perpendicular to the direction of movement of theheating plate 1604.

The force applying member comprises a fixedplate 1606 and acylinder 1608; thefixing plate 1606 and theheating plate 1604 are respectively provided with four through holes, theheating plate 1604 and thefixing plate 1606 are connected through bolts, the screw of each bolt is sleeved with anelastic element 1016, and two ends of theelastic element 1016 are respectively in extrusion contact with the opposite side faces of the fixingplate 1606 and theheating plate 1604; a piston rod of thecylinder 1608 is fixedly connected to thefixing plate 1606; thereciprocating member 1019 comprises a tray 1601, twolinear guide rails 1607, twosliders 1602, a mountingbase 1610 and a synchronousbelt transmission mechanism 1611; the power output end of the synchronousbelt transmission mechanism 1611 is fixedly connected with the tray 1601, and the twosliders 1602 are fixedly mounted on the mountingbase 1610; alinear guide 1607 slidably mounted on aslide 1602; the twolinear guide rails 1607 are respectively fixed on two sides of the tray 1601; asealing plate 1603 is placed on the tray 1601, and a photosensor a1609 for detecting whether thesealing plate 1603 is placed on the tray 1601 is arranged on the tray 1601.

The elastic member is a spring B1605.

Theinstallation base 1610 is installed on theoperation platform 9, be provided with an opening A on theoperation platform 9 ofinstallation base 1610 bottom, be provided with jacking device A1613 on theoperation platform 9 of opening A below, PCR board transports transporting frame A, themembrane sealing board 1603 transports transporting frame B that the module all can pass and install betweeninstallation base 1610 and theoperation platform 9, the moving path that transports frame B is directly over transporting frame A's moving path, jacking device A1613 is used for transporting the PCR board jacking that is located on frame A to transporting to frame B and laying.

Fix the plastic film all around onmembrane sealing plate 1603, heat the plastic film and exert decurrent effort throughhot plate 1604, make the plastic film heat-seal on the sample board, thereby make sample board, plastic film andmembrane sealing plate 1603 form relatively fixed relation of connection, because the hollow size ofmembrane sealing plate 1603 is greater than the hot sealing face size ofhot plate 1604, consequently the hot sealing face ofhot plate 1604 and the frame ofmembrane sealing plate 1603 contactless in the heat-seal process, the frame ofmembrane sealing plate 1603 just can not produce the heat altered shape, because thelocation mouth 1612 sets up on the frame ofmembrane sealing plate 1603, even consequently even at the sample board because the heat altered shape in the heat-seal process, can not influence the location accuracy of follow-up process yet.

As shown in fig. 25 to 26, the gene assaying device comprises a blockingdevice 18, apartition plate 17 for preventing aerosol contamination is provided on theoperation platform 9 between themetal bath module 10 and the heat sealing machine, a through hole is provided on thepartition plate 17, and the blocking device is used for controlling the opening and closing of the through hole.

The blockingdevice 18 comprises a blockingmotor 181, a connectingblock 182, arotating wheel 183, a long connectingrod 184 and abaffle 186,chute module 187,slide rail module 188, the first end of connectingpole 184 is provided with through-going slidinggroove 185,block motor 181,slide rail module 188 is all fixed mounting onoperation platform 9, the pivot of blockingmotor 181 is connected with the one end of connectingblock 182, the other end of connectingblock 182 is connected withrotation wheel 183,rotation wheel 183 is slidable mounting in slidinggroove 185, the second end of connectingpole 184 is connected withbaffle 186,chute module 187 is fixed mounting on the second end lateral wall of connectingpole 184,slide rail module 188 andchute module 187 sliding fit, the direction of slidinggroove 185 is unanimous with the length direction of connectingpole 184, connectingpole 184 is all perpendicular withchute module 187,slide rail module 188, when blockingmotor 181 rotates,baffle 186 promotes or descends,baffle 186 is used for opening or blocking the through-hole.

The blockingmotor 181 in this embodiment is controlled by a microprocessor, and when the PCR plate needs to pass through thepartition 17, the blockingmotor 181 is controlled to operate, the rotating shaft of the blockingmotor 181 drives the connectingblock 182 to rotate, the connectingblock 182 rotates to drive therotating wheel 183 to roll along the slidinggroove 185, and the slidinggroove module 187 slides upwards along the slidingrail module 188 to drive thebaffle 186 to move upwards to expose the through hole on thepartition 17; otherwise, the blockingmotor 181 rotates reversely, and the blockingplate 186 moves downwards to block the through hole on thepartition 17.

As shown in fig. 27 to 31, themetal bath module 10 includes ahot plate assembly 101, thehot plate assembly 101 includes a metalhot plate 1013, the metalhot plate 1013 is provided with a plurality of throughholes 1012, and a central axis of one throughhole 1012 coincides with a central axis of one sample placement position of the PCR plate when the metal bath is performed.

Thehot lid assembly 101 further includes:

a positioning member; the positioning member comprises at least twopositioning pins 1018, the twopositioning pins 1018 and the PCR plate are always in a relatively static state, at least twopositioning holes 1015 are arranged on the metalhot cover 1013, at least onepositioning hole 1015 andother positioning holes 1015 are arranged on different sides of the metalhot cover 1013, and onepositioning pin 1018 is inserted into onepositioning hole 1015 during positioning.

Mounting bracket a1014,spring 1016; theelastic member 1016 has both ends connected to the mounting bracket a1014 and the metalthermal cover 1013, respectively, and when the metalthermal cover 1013 contacts the PCR plate, theelastic member 1016 is restored to the deformed direction in the same direction as the gravity direction.

At least two connectingrods 8621; the first end of each connectingrod 8621 is fixedly connected with the metalthermal cover 1013, the mounting bracket a1014 is provided with a plurality of mounting holes, theelastic member 1016 comprises a plurality ofsprings 8623, one connectingrod 8621 passes through one mounting hole, onespring 8623 is sleeved on the outer wall of one connectingrod 8621, and the cross-sectional dimension of the mounting hole is larger than that of the connectingrod 8621.

At least twostop blocks 1011; the cross-sectional dimension ofstopper 1011 is greater than the cross-sectional dimension of mounting hole, and the second end and thestopper 1011 fixed connection of connectingrod 8621.

Thehot lid assembly 101 includes a movingmember 1019 installed at a lower portion of theoperation platform 9, an opening B is opened at an upper portion of theoperation platform 9 corresponding to the movingmember 1019, a moving path of the transporting frame B passes through a bottom of the metalhot lid 1013, and the movingmember 1019 moves upward to jack up the PCR plate on the transporting frame B of the sealing plate transporting module to be connected with the metalhot lid 1013.

In this embodiment, the plurality of throughholes 1012 are formed in themetal heat cover 1013, and the central axis of each throughhole 1012 coincides with the central axis of the sample placement position of the reaction plate, so that when the reaction plate performs a PCR amplification reaction of a metal bath, light for performing fluorescence detection can reach the gene sample in the sample placement position as it is, and real-time detection of the gene sample during the PCR amplification reaction can be realized, so as to obtain a more comprehensive detection result.

Theoptical inspection instrument 11 comprises anoptical filter device 111, theoptical inspection instrument 11 is mounted on the upper part of theheat cover assembly 101 and acts on the PCR plate after passing through the throughhole 1012;

as shown in fig. 32-37, thelight filtering device 111 includes:

adichroic mirror 1115 for reflecting a light source; the angle of incidence of the light source on thedichroic mirror 1115 is acute;

alight receiving box 1114; thelight receiving box 1114 is used for absorbing the refraction light 1117 on the side, away from thelight receiving box 1114, of the incident light 1117 on thedichroic mirror 1115, thelight receiving box 1114 is provided with a light throughhole 1116 at the upper end, thedichroic mirror 1115 is installed at the upper end of thelight receiving box 1114, and the light 1117 absorption action end and the excitation light source of thelight receiving box 1114 are located on two sides of thedichroic mirror 1115.

The sidewall and the bottom surface inside thelight receiving box 1114 are both subjected to black plastic spraying treatment.

When the light source passes through the two-way mirror 1115, refracted and reflected by the two-way mirror 1115, the refractedlight ray 1117 enters thelight receiving box 1114, black plastic spraying treatment is performed on the side wall and the bottom surface inside thelight receiving box 1114, the refractedlight ray 1117 can be absorbed, the situation that thelight ray 1117 entering thelight receiving box 1114 is mixed with thelight ray 1117 reflected by the two-way mirror 1115 for the first time after being refracted through the reflection of thelight receiving box 1114 is avoided, the accuracy of gene detection is reduced, and therefore the existence of stray light is reduced due to the absorption effect of thelight ray 1117 of thelight receiving box 1114, and the accuracy of gene detection is improved.

The included angle between the bottom surface and the side wall inside thelight receiving box 1114 is not equal to 90 °.

The bottom surface of the interior of thelight receiving box 1114 is obliquely arranged, thelight ray 1117 reaching the bottom surface of thelight receiving box 1114 after refraction is reflected to the side wall of thelight receiving box 1114 again, the light absorption effect of thelight receiving box 1114 is increased, and the detection accuracy is further improved.

Thelight receiving box 1114 is filled with black porous sponge. When the light source passes through the two-way mirror 1115, refracted and reflected by the two-way mirror 1115, the refractedlight ray 1117 enters thelight receiving box 1114, black plastic spraying treatment is performed on the side wall and the bottom surface inside thelight receiving box 1114, the refractedlight ray 1117 can be absorbed, the situation that thelight ray 1117 entering thelight receiving box 1114 is mixed with thelight ray 1117 reflected by the two-way mirror 1115 for the first time after being refracted through the reflection of thelight receiving box 1114 is avoided, the accuracy of gene detection is reduced, and therefore the existence of stray light is reduced due to the absorption effect of thelight ray 1117 of thelight receiving box 1114, and the accuracy of gene detection is improved.

Thelight filtering device 111 further includes:

afirst filter 1111 for filtering other wavelengths of light; the firstoptical filter 1111 is fixedly arranged on the mounting bracket B1112, and the firstoptical filter 1111 is positioned above the two-way mirror 1115;

asecond filter 1113; the upper end of thelight receiving box 1114 is provided with a mounting hole, and the optical filter is mounted in the mounting hole of thelight receiving box 1114;

atotal reflection mirror 1118; the reflecting surface of thetotal reflection mirror 1118 faces thedichroic mirror 1115, and the totally reflectedlight ray 1117 is parallel to the central axis of thePCR plate 95 to be detected.

The initial emission light source filters light 1117 with other wavelengths through the firstoptical filter 1111, reaches the two-way mirror 1115, is reflected and refracted by the two-way mirror 1115, the reflected light 1117 passes through the light-passinghole 1116 in the light-receiving box 1114 to reach thetotal reflection mirror 1118, is reflected by thetotal reflection mirror 1118 to reach thePCR plate 95 and irradiates the gene sample in thePCR plate 95, the light 1117 reaching thePCR plate 95 is parallel to the central axis of thePCR plate 95, the reflected light from the gene sample returns back in the original path, is reflected by thetotal reflection mirror 1118 to reach the two-way mirror 1115, the two-way mirror 1115 refracts and reflects again, since this time the direction of incidence oflight ray 1117 is at the end of the normal todichroic mirror 1115 near thecollection box 1114, therefore, the reflected light 1117 is not absorbed by the light-receiving box 1114, but only a small portion thereof, the refracted light 1117 may be collected by thesecond filter 1113 for subsequent analysis.

The reflecting surface of thetotal reflector 1118 is parallel to the two-way mirror 1115, the acute included angle between the reflecting surface of thetotal reflector 1118 and the two-way mirror 1115 and the gravity direction is 45 degrees, and the reflectedlight ray 1117 of thetotal reflector 1118 is ensured to be parallel to theincident light ray 1117 of the two-way mirror 1115, so that thelight ray 1117 of the initial emission light source only needs to be towards the central axis direction of thePCR plate 95, and thelight ray 1117 reaching thePCR plate 95 can be ensured to be parallel to the central axis of thePCR plate 95.

The central axis of thesecond filter 1113 is perpendicular to the central axis of thelight receiving box 1114.

As shown in fig. 1, 2 and 3, in some embodiments, the gene detection apparatus further comprises ahousing 1, a placement window 2, anobservation window 3, a liquid path unit 4, apower supply unit 5, a recovery port a6 and a recovery port B7, wherein anoperation platform 9 is installed at the lower part in thehousing 1, and the liquid path unit 4 is installed in the back surface of thehousing 1 and is connected with a liquid pipeline on apipetting assembly 8; thepower supply unit 5 is arranged above the inside of theshell 1 and supplies power to all the devices needing power supply of the gene detection device; the recycling port A6 and the recycling port B7 are both arranged on theshell 1, and the recycling port A6 is communicated with the gun head recycling assembly; put thing window 2 and set up in the one end ofcasing 1, be used for putting into purification heating device with reagent through putting thing window 2 in,observation window 3 offer with one side ofcasing 1, can observe movingliquid subassembly 8 throughobservation window 3 and move liquid actuating of machine to and the action of heat-seal machine.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A gene assaying device, comprising:

a purification heating device for nucleic acid purification and extraction;

a pipetting assembly for reagent and sample transfer;

a metal bath module for PCR amplification reaction;

an optical detector for gene detection;

a PCR plate transport module;

a membrane sealing plate conveying module;

a heat sealer for sealing the PCR plate;

2. The gene assaying device according to claim 1, further comprising: the tray module, the tray module includes tray and linear electric motor subassembly A, and linear electric motor subassembly A installs on operation platform to be located between reagent, sample, rifle head processing module and the purification heating device, the tray is connected with linear electric motor subassembly A's removal part, and the tray is synchronous with the lateral shifting of the rifle head on the liquid-transfering component, and when the rifle head on the liquid-transfering component passes through tray module top, the rifle head below is arranged in all the time to the tray.

3. The gene assaying device according to claim 1, wherein the pipetting module comprises:

an X-direction moving means for moving the pipetting unit in the X direction; the X-direction moving assembly comprises an X-direction moving guide rail A, X and an electric cylinder towards the moving guide rail B, X; the X-direction moving guide rail A is parallel to the X-direction moving guide rail B; the Y-direction moving assembly is fixedly arranged on the sliding part of the X-direction electric cylinder;

a Y-direction moving means for moving the pipetting unit in the Y-direction; the Y-direction moving assembly comprises a bearing plate, a support A, a support B, a lead screw A, Y directional moving part, an X-direction motor, a belt pulley A, a belt pulley B and a lead screw sleeve A; the support A and the support B are respectively arranged at two ends of the bearing plate, and two ends of the screw rod A are rotatably arranged on the support A and the support B; the screw rod sleeve A is installed on the Y-direction moving part, the screw rod sleeve A and the screw rod A are installed in a threaded fit mode, the belt pulley B is fixedly connected with one end of the screw rod A, the X-direction motor is installed on the bearing plate, a rotating shaft of the bearing plate is fixedly connected with the belt pulley A, and the belt pulley A and the belt pulley B are in transmission connection through a belt;

wherein, the Y-direction moving component is arranged on the moving part of the X-direction moving component, the Z-direction moving component A is arranged on the moving part of the Y-direction moving component, and the Z-direction moving component B, Z, the Z-direction moving component C and the Z-direction moving component D are both arranged on the moving part of the Z-direction moving component A.

4. The gene assaying device according to claim 3,

the Z-direction moving assembly A comprises a frame A, a transverse plate A, Z directional motor A, a screw rod B, a screw rod sleeve B and a frame B; the frame A is fixedly arranged on a moving part of the Y-direction moving assembly, the transverse plate A is fixedly arranged in the frame A, the Z-direction motor A is arranged on the transverse plate A in an inverted mode, a rotating shaft of the Z-direction motor A penetrates through the transverse plate A to be fixedly connected with one end of the lead screw B, the lead screw sleeve B is installed in a threaded fit mode with the lead screw B, and the lead screw sleeve B is fixedly arranged on the frame B; the frame B can be vertically moved and is arranged in the frame A and is positioned below the transverse plate A;

the Z-direction moving assembly B comprises a Z-direction motor B, a transverse plate B, a guide rod, a screw rod C, a frame C, a screw rod sleeve C and a steel needle group A, wherein the transverse plate B is fixedly arranged in the frame B, the Z-direction motor B is arranged on the transverse plate B in an inverted mode, a rotating shaft of the Z-direction motor B penetrates through the transverse plate B and is fixedly connected with one end of the screw rod C, the screw rod sleeve C and the screw rod C are installed in a threaded fit mode, and the screw rod sleeve C is fixedly arranged on the frame C; the frame C is arranged in the frame B in a vertically movable manner and is positioned below the transverse plate B, the steel needle group A is arranged on the frame C, and the steel needle group A is connected with an external liquid path;

the Z-direction moving assembly C comprises a connecting piece, a shell, a Z-direction motor C, a steel needle group B, a mounting plate, a high-frequency valve, a pipeline winder, a lead screw D and a lead screw sleeve D, wherein the connecting piece is connected with the frame B;

the Z-direction moving assembly D comprises a gun head, a mounting box, a connecting rod, a clamping block, a spring, a piston cavity assembly, a piston assembly, a connecting plate, a screw rod sleeve E, a screw rod E, a frame D, Z directional motor D, a connector and a conical pipe orifice; the frame D is fixedly connected with the frame B, the Z-direction motor D is installed at the upper end of the frame D in an inverted mode, a rotating shaft of the Z-direction motor D penetrates through the upper end of the frame D to be fixedly connected with one end of the screw rod E, the screw rod sleeve E is installed with the screw rod E in a threaded fit mode, the screw rod sleeve E is fixedly installed at the upper end of a shell of the piston assembly, and the piston assembly can be installed in the frame D in a vertically moving mode; the piston cavity assembly is fixedly arranged in the frame D, is arranged below the piston assembly and is installed in a matching way; the gun head is arranged below the piston cavity assembly and is communicated and matched with the piston cavity assembly;

the Z-direction moving assembly D also comprises a mounting box, a connecting rod, a clamping block, a spring, a connecting plate and a connector; the connecting plate is arranged at the lower end of the piston assembly, a stepped through hole is vertically formed in the connecting plate, the diameter of the upper portion of the stepped through hole is larger than that of the lower portion of the stepped through hole, the upper end of the connecting rod is connected with a fixture block, the lower end of the connecting rod is fixedly connected with the mounting box, the diameter of the fixture block is slightly smaller than that of the stepped through hole, the spring is sleeved on the connecting rod, and the spring is mounted between a step of the stepped through hole and the fixture block; the mounting box is provided with a plurality of through holes A, a plurality of connectors are slidably mounted in the through holes A, the lower ends of the connectors are provided with conical pipe orifices, and the conical pipe orifices are inserted into the upper ends of the gun heads for mounting; when the piston assembly is pressed downwards to the bottom, the piston assembly extrudes the clamping block downwards, the spring is compressed, the clamping block slides into the upper hole of the stepped through hole, the connecting rod pushes the mounting box to move downwards, and the mounting box pushes the gun head to be separated from the conical pipe orifice.

5. The gene detection device according to claim 4, wherein the reagent, sample and gun head processing module comprises a gun head storage module, a sample cooling module, a reagent cooling module A, a reagent cooling module B, a needle cleaning module A, a needle cleaning module B, a positive reference object cooling module and a gun head recovery assembly for gun head storage, and the gun head storage module, the sample cooling module, the reagent cooling module A, the needle cleaning module B, the reagent cooling module B, the positive reference object cooling module and the gun head recovery assembly are all arranged on the operation platform; the needle head cleaning module A is used for cleaning the steel needle group A, and the needle head cleaning module B is used for cleaning the steel needle group B.

6. The gene assaying device according to claim 1, wherein the purification heating means comprises:

at least one first heating element; the heating action end of the first heating element acts on the gene sample with the same heating requirement in the sample box;

a first heating chamber; the first heating element is arranged in the first heating chamber;

a second heating member; the heating action ends of the second heating elements act on all the gene samples in the sample box, and the number of the gene samples acted by the heating action ends of the first heating elements is less than that of the gene samples acted by the heating action ends of the second heating elements;

a second heating chamber; the second heating member is installed in the second heating chamber, and when carrying out the gene sample heating of the heating demand of the same race, the sample box is arranged in first heating chamber, and when carrying out the heating of whole gene samples, the second heating chamber is arranged in to the sample box.

7. The gene assaying device according to claim 6,

the second heating element is a heating base, a heating sheet is arranged in the heating base, and when all gene samples are heated, the upper surface of the heating base is in contact with the bottom of the sample box to heat all the gene samples in the sample box;

the upper surface of the heating base is provided with a plurality of heating grooves, the bottom of each sample placing position of the sample box protrudes outwards to form a boss, and one heating groove is in contact with the boss at the bottom of one sample placing position to heat the gene sample in the sample placing position;

the heating groove on the heating base and the boss at the bottom of the sample placing position are both hemispherical;

the first heating element comprises a metal block, a heating rod is arranged in the metal block, and when the gene sample with the same heating requirement is heated, the upper surface of the metal block is contacted with the bottom of the sample position needing to be heated in the sample box to heat the gene sample in the sample position;

the upper surface of the metal block is provided with a plurality of heating grooves, the bottom of each sample placing position of the sample box protrudes outwards to form a boss, and one heating groove is in contact with the boss at the bottom of one sample placing position to heat the gene sample in the sample placing position;

the heating groove on the metal block and the boss at the bottom of the sample placing position are both hemispherical.

8. The gene assaying device according to claim 1, wherein the heat sealing machine comprises:

sealing the membrane plate; the film sealing plate is of a hollow structure, the middle part of the film sealing plate is fixedly provided with a plastic film for sealing the film of the sample plate, and the film sealing plate is provided with at least two positioning ports;

a heating plate for heating the plastic film; the hollow size of the film sealing plate is larger than the size of the heat sealing surface of the heating plate, and the sample plate is positioned right below the heat sealing surface of the heating plate;

the force application part is used for controlling the heating plate to be far away from or close to the film sealing plate;

a reciprocating member for moving the sealing membrane plate to a position between the heating plate and the sample plate; the moving direction of the film sealing plate is vertical to the moving direction of the heating plate.

9. The gene assaying device according to claim 8, wherein the force applying member comprises a fixing plate, a cylinder; the fixed plate and the heating plate are respectively provided with four through holes, the heating plate is connected with the fixed plate through bolts, the screw rod of each bolt is sleeved with an elastic part, and two ends of the elastic part are respectively in extrusion contact with the opposite side surfaces of the fixed plate and the heating plate; a piston rod of the cylinder is fixedly connected with the fixed plate; the reciprocating moving piece comprises a tray, two linear guide rails, two sliding blocks, a mounting base and a synchronous belt transmission mechanism; the power output end of the synchronous belt transmission mechanism is fixedly connected with the tray, and the two sliding blocks are fixedly arranged on the mounting base; a linear guide rail is slidably mounted on a slide block; the two linear guide rails are respectively fixed on two sides of the tray; the film sealing plate is placed on the tray, and the tray is provided with a photoelectric sensor for detecting whether the film sealing plate is placed on the tray or not.

10. The gene detecting apparatus according to claim 9, wherein the mounting base is mounted on the operation platform, the operation platform is provided with an opening a at the bottom of the mounting base, the operation platform is provided with a lifting device a below the opening a, the transporting frame a of the PCR plate transporting module and the transporting frame B of the membrane sealing plate transporting module can pass through the mounting base and the operation platform, the moving path of the transporting frame B is right above the moving path of the transporting frame a, and the lifting device a is used for lifting the PCR plate on the transporting frame a onto the transporting frame B for placement.