CN114134641A - Preparation method of keratin and nylon 66 nanofiber membrane - Google Patents

Abstract

Translated fromChinese

本发明公开了一种角蛋白和尼龙66纳米纤维膜制备方法，包括步骤一，利用六氟异丙醇溶剂和角蛋白粉末配置成一定浓度的角蛋白溶液；步骤二利用六氟异丙醇溶剂和尼龙66配置成一定浓度的尼龙66溶液；步骤三，将尼龙66溶液和角蛋白溶液混合静置脱泡，步骤四，将混合溶液通过静电纺丝机器制备出复合纳米纤维。该角蛋白和尼龙66纳米纤维膜制备方法，角蛋白资源可从废弃资源中提出再利用，配置成角蛋白溶液，再将其他待纺溶液加入到角蛋白溶液中混合均匀后得到纺丝溶液，通过静电纺丝技术，静电纺丝出纳米纤维纤维，提取出的角蛋白/尼龙66纳米纤维膜有着优良的力学性能和可降解性，角蛋白在纳米纤维、医学和污水等领域有着巨大的应用前景。

The invention discloses a method for preparing a keratin and nylon 66 nanofiber membrane. The method comprises the first step of preparing a keratin solution of a certain concentration by using a hexafluoroisopropanol solvent and keratin powder; and in the second step, using a hexafluoroisopropanol solvent and nylon 66 to form a nylon 66 solution with a certain concentration; step 3, mix the nylon 66 solution and the keratin solution to stand for defoaming, and step 4, pass the mixed solution through an electrospinning machine to prepare composite nanofibers. According to the preparation method of keratin and nylon 66 nanofiber membrane, keratin resources can be extracted from waste resources and reused, and configured into a keratin solution, and then other solutions to be spun are added to the keratin solution and mixed evenly to obtain a spinning solution, Through electrospinning technology, electrospinning nanofiber fibers, the extracted keratin/nylon 66 nanofiber membrane has excellent mechanical properties and degradability, and keratin has a huge application in the fields of nanofibers, medicine and sewage prospect.

Description

Preparation method of keratin and nylon 66 nanofiber membrane

Technical Field

The invention relates to the technical field of biotechnology, in particular to a preparation method of a keratin and nylon 66 nanofiber membrane.

Background

At present, the invention and the utilization of novel materials are more important in China, and the nanofiber membrane is mainly used for filtration, such as gas or liquid, is generally used for gas filtration, and can filter most microorganisms or organic matters and the like, so that the purification effect is achieved, and meanwhile, along with the rapid improvement of the life of people, the requirement of the fiber membrane is also increased year by year, so that some problems are caused:

the common fiber membrane is made of materials such as polypropylene, resin, diatomite or textile fabrics, the demand is huge, the consumption amount is huge, and the common fiber membrane cannot be made of materials extracted from more waste resources, so that the resource recovery rate is low.

Disclosure of Invention

The invention aims to provide a preparation method of a keratin and nylon 66 nano fiber membrane, so as to achieve the purpose of utilizing waste resources to prepare the fiber membrane in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a keratin and nylon 66 nanofiber membrane comprises the following steps:

weighing a certain amount of keratin powder, and preparing a keratin solution with a certain concentration by using a hexafluoroisopropanol solvent;

weighing a certain amount of nylon 66, and preparing a nylon 66 solution with a certain concentration by using a hexafluoroisopropanol solvent;

step three, mixing the solutions obtained in the step one and the step two, standing and defoaming to obtain a mixed solution, wherein the obtained mixed solution is a spinning solution;

and step four, preparing the composite nano fiber from the mixed solution obtained in the step three by using an electrostatic spinning technology through an electrostatic spinning machine.

Preferably, in the first step, the keratin powder is prepared from pig nail keratin.

Preferably, in the first step, the keratin powder is dissolved with hexafluoroisopropanol to obtain a keratin solution with a concentration of 4%.

Preferably, in the second step, nylon 66 is dissolved by hexafluoroisopropanol to obtain a nylon 66 solution with a concentration of 12%.

Preferably, in the third step, the two solutions are mixed to obtain a spinning solution, and the volume ratio of the keratin solution to the nylon 66 solution is 1: 1.

Preferably, in the fourth step, the voltage of the electrostatic spinning machine is set to 25 kv, the receiving distance is 12 cm, and the spinning flow rate is 0.2 ml/h.

Compared with the prior art, the invention has the beneficial effects that: the preparation method of the keratin and nylon 66 nanofiber membrane comprises the following steps:

the keratin resource can be extracted from waste resources for reuse, then keratin solution is prepared, other solutions to be spun are added into the keratin solution and mixed evenly to obtain spinning solution, the nanofiber fiber is obtained by electrostatic spinning through the electrostatic spinning technology, and the extracted keratin/nylon 66 nanofiber membrane has excellent mechanical property and excellent degradability, so that the keratin has huge application prospect in the fields of nanofiber, medicine, sewage, biological packaging materials and the like.

Drawings

FIG. 1 is a schematic view of a scanning electron microscope of a nanofiber membrane of the present invention;

FIG. 2 is a schematic diagram of a transmission electron microscope of the nanofiber membrane of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, the present invention provides a technical solution: a method for preparing a keratin and nylon 66 nanofiber membrane, comprising:

the method comprises the following steps:

weighing a certain amount of keratin powder, and preparing a keratin solution with a certain concentration by using a hexafluoroisopropanol solvent;

weighing a certain amount of nylon 66, and preparing a nylon 66 solution with a certain concentration by using a hexafluoroisopropanol solvent;

step three, mixing the solutions obtained in the step one and the step two, standing and defoaming to obtain a mixed solution, wherein the obtained mixed solution is a spinning solution;

and step four, preparing the composite nano fiber from the mixed solution obtained in the step three by using an electrostatic spinning technology through an electrostatic spinning machine.

In the first step, the keratin powder is prepared from pig nail keratin.

In the first step, the keratin powder is dissolved with hexafluoroisopropanol to obtain a keratin solution with a concentration of 4%.

In the second step, nylon 66 is dissolved by hexafluoroisopropanol to obtain a nylon 66 solution with the concentration of 12%.

And in the third step, mixing the two solutions to obtain a spinning solution, wherein the volume ratio of the keratin solution to the nylon 66 solution is 1: 1.

In the fourth step, the voltage of the electrospinning machine was set to 25 kv, the take-up distance was 12 cm, and the spinning flow rate was 0.2 ml/h.

Firstly, providing a reusable keratin resource from waste resources, preparing a keratin solution, adding other solutions to be spun into the keratin solution, uniformly mixing to obtain a spinning solution, and carrying out electrostatic spinning on the nanofiber fiber by using an electrostatic spinning technology, wherein the prepared spinning solution has the following conditions: the molecular weight of the keratin must not be too small; the high molecular material used for mixing can not generate denaturation reaction with keratin; the mechanical property of the composite fiber prepared by spinning is higher than that of the fiber spun by pure high polymer material.

The main operation flow of the electrostatic spinning technology is as follows: the prepared spinning solution is added into an injector, the injector is fixed and connected with a power supply, the spinning solution is pushed out of the injector under the action of external force, when the power supply is adjusted to be large enough, the spinning solution is sprayed on a receiving plate in a filament shape, and a layer of fiber membrane is gradually formed on the receiving plate along with the time, wherein the power supply in the device generally adopts a positive and negative double power supply, the injector is generally a glass injector, and the receiving plate is generally aluminum foil paper.

The working principle is as follows: the keratin and nylon 66 nanofiber membrane preparation method comprises the steps of weighing a certain amount of keratin powder and PA66 respectively, dissolving the keratin powder and the PA66 in hexafluoroisopropanol, enabling the concentration of a keratin solution to be 4%, preparing a PA66 solution with the mass fraction of 12%, uniformly mixing the two solutions to obtain a spinning solution, placing the prepared spinning solution on a magnetic stirrer, stirring for 1 hour, standing for defoaming, pouring the defoamed spinning solution into a 10 ml injector, preparing a nanofiber membrane on an electrostatic spinning machine, spinning the nanofiber membrane with a certain thickness on an aluminum foil at room temperature, setting the voltage of the electrostatic spinning machine to be 25 kv, setting the acceptance distance to be 12 cm, setting the spinning flow to be 0.2 ml/h, and increasing the overall practicability.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. A preparation method of a keratin and nylon 66 nanofiber membrane is characterized by comprising the following steps:

weighing a certain amount of keratin powder, and preparing a keratin solution with a certain concentration by using a hexafluoroisopropanol solvent;

weighing a certain amount of nylon 66, and preparing a nylon 66 solution with a certain concentration by using a hexafluoroisopropanol solvent;

step three, mixing the solutions obtained in the step one and the step two, standing and defoaming to obtain a mixed solution, wherein the obtained mixed solution is a spinning solution;

and step four, preparing the composite nano fiber from the mixed solution obtained in the step three by using an electrostatic spinning technology through an electrostatic spinning machine.

2. The method of claim 1, wherein the membrane is prepared from keratin and nylon 66 nanofibers by: in the first step, the keratin powder is prepared from pig nail keratin.

3. The method of claim 1, wherein the membrane is prepared from keratin and nylon 66 nanofibers by: in the first step, the keratin powder is dissolved by hexafluoroisopropanol to obtain a keratin solution with a concentration of 4%.

4. The method of claim 1, wherein the membrane is prepared from keratin and nylon 66 nanofibers by: in the second step, the nylon 66 is dissolved by hexafluoroisopropanol to obtain a nylon 66 solution with the concentration of 12%.

5. The method of claim 1, wherein the membrane is prepared from keratin and nylon 66 nanofibers by: and in the third step, mixing the two solutions to obtain a spinning solution, wherein the volume ratio of the keratin solution to the nylon 66 solution is 1: 1.

6. The method of claim 1, wherein the membrane is prepared from keratin and nylon 66 nanofibers by: in the fourth step, the voltage of the electrostatic spinning machine is set to be 25 kv, the receiving distance is 12 cm, and the spinning flow rate is 0.2 ml/h.

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