Multilayer functional wound dressing prepared by adopting portable electrospinning technologyTechnical Field
The invention belongs to the technical field of wound dressings, and particularly relates to a multilayer functional wound dressing prepared by adopting a portable electrospinning technology.
Background
As a simple and efficient process capable of producing hyperfine fibers, the electrostatic spinning technology is widely applied to the fields of tissue engineering, drug loading, medical dressing and the like, and is a most promising process method for the accepted industrialization of the nanofibers.
In recent years, the portable electrostatic spinning device has made great progress, the volume is greatly reduced, the portable electrostatic spinning device is easy to carry, the shape of the electrostatic spinning dressing can be controlled, namely the electrostatic spinning dressing is formed by in-situ deposition in a few minutes at a wound, and the portable electrostatic spinning device is suitable for various irregular-shaped wounds.
However, the materials for electrostatic spinning available for portable electrostatic spinning are not many, and the materials mainly used at present are polyvinylpyrrolidone, polylactic acid and the like. The Xiiaoei Liu et al uses the technology to dissolve the polyvinylpyrrolidone and the gelatin in the acetic acid for electrostatic spinning to form the nanofiber dressing; hongrang Chen et al used this technique to electrospun nanofiber dressings from polylactic acid and gelatin dissolved in hexafluoroisopropanol. It can be seen that this technique relies on solution electrospinning, which poses a risk of organic solvents remaining in the fibres, possibly affecting wound healing.
Therefore, the invention adopts the portable electrostatic spinning device to prepare the multilayer functional wound dressing, the used materials have good biocompatibility, and the polyvinyl butyral which is a new material capable of being used as the wound dressing by electrostatic spinning is developed. The adopted solvents are all green and non-toxic, and the influence of solvent residues on wounds is avoided.
One problem faced by current wound dressings is that although hydrophilic dressings can absorb wound exudate, they may over-wet the wound due to their wet surface, affecting the wound healing process, whereas hydrophobic wound dressings may prevent the external environment from affecting the wound, but have no effect of removing wound exudate.
Therefore, the dressing constructs an asymmetric multifunctional structure with hydrophilic and hydrophobic properties, and the hydrophilic and hydrophobic layer close to the wound can absorb wound exudate from bottom to top to the hydrophilic liquid absorption layer to achieve the effect of controlling the wettability of the surface of the wound, so that a relatively dry healing environment is provided for the wound, the excessive water supplement of the wound is avoided, and the healing of the wound is promoted. The invention additionally adds antibacterial agents, antioxidants and other substances, can achieve the effects of antibiosis, antiphlogosis, antioxidation and the like, and has high practical application value in the aspect of wound treatment.
Disclosure of Invention
Aiming at the problems that the dressing prepared at present cannot be individually attached to the shape of a wound and the preparation machine equipment is huge, and the requirements of using a non-toxic solvent and preparing a nanofiber dressing with good individual performance in a convenient mode, the invention prepares a multilayer functional wound dressing by using a portable electrostatic spinning device. The wound dressing can be used for conveniently treating wounds of various shapes, a multi-layer functional wound dressing is formed in situ at the wound, the dressing can provide a relatively dry healing environment for the wound, and the wound healing process is prevented from being influenced by excessive wound exudates.
In order to solve the technical problems and meet the technical requirements, the invention adopts the following technical scheme:
(1) preparing a skin-friendly hydrophobic layer spinning solution: sequentially adding polyvinyl butyral, polyvinylpyrrolidone, quercetin and vitamin K2 into isopropanol, stirring at normal temperature until the materials are fully dissolved, and preparing into skin-friendly hydrophobic layer spinning solution called precursor solution A. The skin-friendly hydrophobic layer has excellent hydrophobicity, and can prevent wound exudate from soaking skin around a wound, so that the risk of infection is reduced, and meanwhile, quercetin in the hydrophobic layer has multiple biological activities, such as antioxidant, antiviral, anti-inflammatory and the like, and can reduce inflammatory reaction of the wound;
(2) preparing a hydrophilic liquid absorbing layer spinning solution: adding polyvinylpyrrolidone, sodium polyacrylate and water-soluble chitosan into an ethanol water solution, stirring at normal temperature until the mixture is uniformly mixed, and preparing into a hydrophilic liquid absorption layer spinning solution which is called precursor solution B. The hydrophilic liquid absorption layer is a driving source for absorbing wound exudate, can guide the wound exudate into the hydrophilic absorption layer without flowing back into the wound, and meanwhile, the water-soluble chitosan has antibacterial property and can prevent the proliferation of pathogenic bacteria;
(3) preparing a hydrophobic anti-sticking layer spinning solution: sequentially adding polyvinyl butyral, ethyl cellulose and quercetin into absolute ethyl alcohol, stirring at normal temperature until the polyvinyl butyral, the ethyl cellulose and the quercetin are fully dissolved, and preparing the hydrophobic anti-sticking layer spinning solution which is called precursor solution C. The hydrophobic anti-sticking layer can prevent the hydrophilic layer from sticking with the clothes to pollute the clothes, and simultaneously, the air permeability of the wound dressing is increased;
(4) preparation of multilayer functionalized wound dressing: the precursor solutions A, B, C are separately drawn using syringes. And sequentially putting the precursor solution A, B, C into a portable electrostatic spinning device to spin on the wound to obtain the multilayer functional portable electrospun wound dressing. The multifunctional three-layer structure ensures that the surface of the wound is dry and comfortable, the wound environment is moist, the air permeability is kept, the rapid healing of the wound is facilitated, and the simple and portable preparation method of the wound dressing enables the medical clinical personalized wound dressing for wound attachment to become possible, so that the preparation method has a good prospect.
The specific embodiment mode is as follows:
in order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the description of the specific embodiments is intended for purposes of illustration only and is not intended to limit the scope of the present disclosure. In addition, the technical features related to the embodiments of the present invention described below may be combined with each other as long as they are in conflict with each other.
All of the starting materials of the present invention, without particular limitation as to their source, may be purchased commercially or prepared according to conventional methods well known to those skilled in the art.
Example 1
A method of making a multi-layer functionalized portable electrospun wound dressing, the method comprising the steps of:
(1) preparing a skin-friendly hydrophobic layer spinning solution: sequentially adding 8 parts of polyvinyl butyral, 2 parts of polyvinylpyrrolidone, 1 part of quercetin and 21 parts of vitamin K into 88 parts of isopropanol, stirring at normal temperature until the materials are fully dissolved, and preparing a skin-friendly hydrophobic layer spinning solution which is called a precursor solution A;
(2) preparing a hydrophilic liquid absorbing layer spinning solution: adding 10 parts of polyvinylpyrrolidone, 1 part of sodium polyacrylate and 2 parts of water-soluble chitosan into an ethanol aqueous solution containing 58 parts of absolute ethanol and 29 parts of purified water, stirring at normal temperature until the mixture is uniformly mixed, and preparing a hydrophilic liquid absorption layer spinning solution which is called a precursor solution B;
(3) preparing a hydrophobic anti-sticking layer spinning solution: sequentially adding 12 parts of polyvinyl butyral, 3 parts of ethyl cellulose and 1 part of quercetin into 84 parts of absolute ethyl alcohol, stirring at normal temperature until the materials are fully dissolved, and preparing a hydrophobic anti-sticking layer spinning solution which is called a precursor solution C;
(4) preparation of multilayer functionalized wound dressing: extracting 1 ml of precursor solution A by using an injector, putting the injector into portable electrostatic spinning equipment, carrying out portable electrostatic spinning at a position 5 cm away from a skin wound, finishing spinning after 1 min, and removing the injector; then taking a new injector to extract 1 ml of precursor liquid B, and repeating the spinning step; and then taking a new injector to extract 1 ml of the precursor solution C, and continuously repeating the spinning steps to prepare the multi-layer functional portable electrospinning wound dressing, wherein the parameters of the portable electrospinning are that the spinning voltage is 10 kV, and the model of a needle head is 23G.
Example 2
A method of making a multi-layer functionalized portable electrospun wound dressing, the method comprising the steps of:
(1) preparing a skin-friendly hydrophobic layer spinning solution: sequentially adding 20 parts of polyvinyl butyral, 5 parts of polyvinylpyrrolidone, 3 parts of quercetin and 22 parts of vitamin K into 70 parts of isopropanol, stirring at normal temperature until the materials are fully dissolved, and preparing a skin-friendly hydrophobic layer spinning solution which is called a precursor solution A;
(2) preparing a hydrophilic liquid absorbing layer spinning solution: adding 12 parts of polyvinylpyrrolidone, 2 parts of sodium polyacrylate and 5 parts of water-soluble chitosan into ethanol aqueous solution of 54 parts of absolute ethanol and 27 parts of purified water, stirring at normal temperature until the mixture is uniformly mixed, and preparing hydrophilic liquid absorption layer spinning solution which is called precursor solution B;
(3) preparing a hydrophobic anti-sticking layer spinning solution: sequentially adding 20 parts of polyvinyl butyral, 5 parts of ethyl cellulose and 4 parts of quercetin into 71 parts of absolute ethyl alcohol, and stirring at normal temperature until the materials are fully dissolved to prepare a hydrophobic anti-sticking layer spinning solution which is called as a precursor solution C;
(4) portable electrostatic spinning: extracting 1 ml of precursor solution A by using an injector, putting the injector into portable electrostatic spinning equipment, carrying out portable electrostatic spinning at a position 5 cm away from a skin wound, finishing spinning after 1 min, and removing the injector; then taking a new injector to extract 1 ml of precursor liquid B, and repeating the spinning step; and then taking a new injector to extract 1 ml of the precursor solution C, and continuously repeating the spinning step to obtain the multilayer functional portable electrospinning wound dressing. The parameters of the portable electrostatic spinning are that the spinning voltage is 8 kV, and the type of the needle head is 21G.
Example 3
A method of making a multi-layer functionalized portable electrospun wound dressing, the method comprising the steps of:
(1) preparing a skin-friendly hydrophobic layer spinning solution: sequentially adding 8 parts of polyvinyl butyral, 5 parts of polyvinylpyrrolidone, 2 parts of quercetin and 22 parts of vitamin K into 83 parts of isopropanol, stirring at normal temperature until the materials are fully dissolved, and preparing a skin-friendly hydrophobic layer spinning solution which is called a precursor solution A;
(2) preparing a hydrophilic liquid absorbing layer spinning solution: adding 14 parts of polyvinylpyrrolidone, 3 parts of sodium polyacrylate and 5 parts of water-soluble chitosan into an ethanol aqueous solution containing 52 parts of absolute ethanol and 26 parts of purified water, stirring at normal temperature until the mixture is uniformly mixed, and preparing a hydrophilic liquid absorption layer spinning solution which is called a precursor solution B;
(3) preparing a hydrophobic anti-sticking layer spinning solution: sequentially adding 12 parts of polyvinyl butyral, 4 parts of ethyl cellulose and 2 parts of quercetin into 82 parts of absolute ethyl alcohol, and stirring at normal temperature until the materials are fully dissolved to prepare a hydrophobic anti-sticking layer spinning solution which is called a precursor solution C;
(4) portable electrostatic spinning: extracting 1 ml of precursor solution A by using an injector, putting the injector into portable electrostatic spinning equipment, carrying out portable electrostatic spinning at a position 5 cm away from a skin wound, finishing spinning after 1 min, and removing the injector; then taking a new injector to extract 1 ml of precursor liquid B, and repeating the spinning step; and then taking a new injector to extract 1 ml of the precursor solution C, and continuously repeating the spinning step to obtain the multilayer functional portable electrospinning wound dressing. The parameters of the portable electrostatic spinning are that the spinning voltage is 14 kV, and the needle type is 22G.