CN113144428B - Multichannel wireless optogenetic stimulation system and method - Google Patents

Abstract

Translated fromChinese

本发明提供一种多通道无线光遗传刺激系统及方法，包括上位机、光刺激器以及阵列式刺激光极，所述光刺激器包括多个刺激通道，不同刺激通道与阵列式刺激光极的不同区域电连通，所述上位机用于设置光刺激参数，所述光刺激参数包括脉冲的频率、占空比、刺激时间和一个或多个刺激通道，所述上位机和光刺激器无线连接，所述光刺激器接收上位机设置的光刺激参数，输出电脉冲信号给光刺激参数中刺激通道电连通的阵列式刺激光极的区域。上述方法及系统能独立选择刺激的区域。

The present invention provides a multi-channel wireless optogenetic stimulation system and method, including a host computer, a light stimulator, and an array-type stimulating light pole. Different areas are electrically connected, and the host computer is used to set photostimulation parameters, which include pulse frequency, duty cycle, stimulation time and one or more stimulation channels, and the host computer is wirelessly connected to the photostimulator, The optical stimulator receives the optical stimulation parameters set by the host computer, and outputs an electric pulse signal to the area of the array stimulation light electrodes electrically connected to the stimulation channel in the optical stimulation parameters. The method and system described above can independently select the area to be stimulated.

Description

Translated fromChinese

多通道无线光遗传刺激系统及方法Multi-channel wireless optogenetic stimulation system and method

技术领域technical field

本发明涉及光刺激技术领域，更为具体地，涉及一种多通道无线光遗传 刺激系统及方法。The present invention relates to the technical field of optical stimulation, and more specifically, to a multi-channel wireless optogenetic stimulation system and method.

背景技术Background technique

光遗传技术是结合光学和遗传学的一种新技术，可以利用光控方式准确 调控特定神经元的活动。通过用病毒载体将光敏蛋白导入到特定的细胞中表 达，被导入的光敏蛋白只能被特定波长的光激活，因此可以利用相应波长的 光照激活或者抑制离子通道实现对神经细胞功能的精确调控。时间准确程度 可达到毫秒范围，在空间上可实现对单一细胞甚至亚细胞范围的精确控制。 近年来，该技术在神经科学研究中非常受欢迎，并将引领下一代神经修复方 法和神经治疗。Optogenetic technology is a new technology combining optics and genetics, which can accurately regulate the activity of specific neurons by means of light control. By using viral vectors to introduce light-sensitive proteins into specific cells for expression, the introduced light-sensitive proteins can only be activated by light of specific wavelengths, so light of corresponding wavelengths can be used to activate or inhibit ion channels to achieve precise regulation of nerve cell functions. The time accuracy can reach the range of milliseconds, and the precise control of a single cell or even a subcellular range can be realized spatially. In recent years, this technique has become very popular in neuroscience research and will lead to the next generation of neural repair methods and neurotherapy.

为了更好的认识脑网络和脑功能，研究人员一般采用构建体外神经网络 的方式。针对光遗传技术体外刺激的需求，研究人员一般采用LED作为光源 开发光刺激器。目前多数体外光遗传刺激器采用LED作为光源，设备体积较 大，所能提供的光照面积大，所能提供的刺激精度有限。光刺激器的调控依 靠有线连接，且供调控的参数较少，不能独立选择刺激的区域。由于设备功 率相对较高，长时间的使用会引起设备发热，从而影响神经细胞的活性，不利于神经细胞的生长。In order to better understand brain network and brain function, researchers generally adopt the method of constructing neural network in vitro. In response to the demand for in vitro stimulation of optogenetic technology, researchers generally use LEDs as light sources to develop light stimulators. At present, most optogenetic stimulators in vitro use LEDs as light sources. The equipment is relatively large in size and can provide a large area of illumination, but the stimulation accuracy it can provide is limited. The regulation of the optical stimulator depends on the wired connection, and there are few parameters for regulation, and the stimulated area cannot be independently selected. Due to the relatively high power of the device, long-term use will cause the device to heat up, which will affect the activity of nerve cells and is not conducive to the growth of nerve cells.

发明内容Contents of the invention

鉴于上述问题，本发明的目的是提供一种能独立选择刺激的区域的多通 道无线光遗传刺激系统及方法。In view of the above problems, the object of the present invention is to provide a multi-channel wireless optogenetic stimulation system and method that can independently select the area to be stimulated.

为了实现上述目的，本发明提供一种多通道无线光遗传刺激系统，包括 上位机、光刺激器以及阵列式刺激光极，所述光刺激器包括多个刺激通道， 不同刺激通道与阵列式刺激光极的不同区域电连通，所述上位机用于设置光 刺激参数，所述光刺激参数包括脉冲的频率、占空比、刺激时间和一个或多 个刺激通道，所述上位机和光刺激器无线连接，所述光刺激器接收上位机设 置的光刺激参数，输出电脉冲信号给光刺激参数中刺激通道电连通的阵列式 刺激光极的区域。In order to achieve the above object, the present invention provides a multi-channel wireless optogenetic stimulation system, including a host computer, a light stimulator, and an array stimulation optode, the light stimulator includes a plurality of stimulation channels, different stimulation channels and array stimulation The different regions of the optode are electrically connected, and the host computer is used to set the light stimulation parameters, the light stimulation parameters include pulse frequency, duty cycle, stimulation time and one or more stimulation channels, the host computer and the light stimulator Wireless connection, the optical stimulator receives the optical stimulation parameters set by the host computer, and outputs the electric pulse signal to the array stimulation light electrode area electrically connected to the stimulation channel in the optical stimulation parameters.

可选地，还包括无线发送模块和无线接收模块，所述无线发送模块连接 上位机和无线接收模块，所述无线接收模块连接无线发送模块和光刺激器， 所述无线发送模块将上位机的光刺激参数通过无线接收模块发送给光刺激 器。Optionally, it also includes a wireless sending module and a wireless receiving module, the wireless sending module is connected to the host computer and the wireless receiving module, the wireless receiving module is connected to the wireless sending module and the light stimulator, and the wireless sending module connects the light of the upper computer Stimulation parameters are sent to the optical stimulator through the wireless receiving module.

可选地，所述无线发送模块包括射频放大以及高增益天线。Optionally, the wireless sending module includes a radio frequency amplifier and a high-gain antenna.

可选地，所述阵列式刺激光极包括光极底板以及多个光极，用于发出光 线对神经细胞进行光刺激。Optionally, the array stimulating light pole includes a light pole bottom plate and a plurality of light poles, which are used to emit light to stimulate nerve cells.

可选地，所述光极底板基底材料采用硅基底，且表面镀有一层环氧树脂 薄膜。Optionally, the base material of the optode base plate is a silicon base, and the surface is coated with a layer of epoxy resin film.

可选地，所述光极为μLED。Optionally, the light poles are μLEDs.

可选地，所述阵列式刺激光极包括24个光极，光刺激器包括6个光刺激 通道，每四个光极为一个区域，每一个区域通过一个光刺激通道控制。Optionally, the array stimulation optode includes 24 optodes, and the optical stimulator includes 6 optical stimulation channels, every four optical poles is an area, and each area is controlled by an optical stimulation channel.

可选地，所述光刺激器包括电源驱动电路、开关、时钟电路、指示灯、 无线通信接口、第二微控制器、复位电路以及输出电路，电源驱动电路用于 供电，开关用于光刺激器的开启与关闭，时钟电路用于为第二微控制器提供 时钟信号，指示灯包括电源指示灯以及通信状态指示灯，电源指示灯用于显 示光刺激器的开启与关闭状态，亮则表示光刺激器在工作，通信状态指示灯 用于显示光刺激器与上位机的通信状态，当光刺激器成功接收到上位机发送 来的信号后，通信状态指示灯将发生亮暗变化，无线通信接口用于连接上位 机与光刺激器，第二微控制器用于提取光刺激参数，经过处理后，以电脉冲 信号形式传递给输出电路，输出电路用于连接光刺激器与阵列式刺激光极， 将第二微控制器输出的电脉冲信号传递给光极，复位电路用于将光刺激器恢 复到初始化状态。Optionally, the light stimulator includes a power drive circuit, a switch, a clock circuit, an indicator light, a wireless communication interface, a second microcontroller, a reset circuit and an output circuit, the power drive circuit is used for power supply, and the switch is used for light stimulation The clock circuit is used to provide a clock signal for the second micro-controller. The indicator light includes a power indicator light and a communication status indicator light. The power indicator light is used to display the on and off status of the optical stimulator. The optical stimulator is working, and the communication status indicator is used to display the communication status between the optical stimulator and the host computer. When the optical stimulator successfully receives the signal sent by the host computer, the communication status indicator will change from bright to dark, wireless communication The interface is used to connect the upper computer and the optical stimulator, and the second microcontroller is used to extract the optical stimulation parameters. After processing, it is transmitted to the output circuit in the form of electric pulse signal, and the output circuit is used to connect the optical stimulator and the array stimulation light electrode. , transmit the electric pulse signal output by the second micro-controller to the light electrode, and the reset circuit is used to restore the light stimulator to the initialization state.

可选地，所述上位机包括参数设置模块、状态显示模块和操作按钮，所 述参数设置模块用于设置光刺激参数，所述状态显示模块用于显示当前光刺 激的频率、占空比以及剩余刺激时间，所述操作按钮包括开始刺激按钮、停 止刺激按钮以及开关键；开关键用于控制刺激通道的启闭，开始刺激按钮用 于发送开始刺激的指令，停止刺激按钮用于发送停止刺激的指令。Optionally, the host computer includes a parameter setting module, a status display module and operation buttons, the parameter setting module is used to set the optical stimulation parameters, and the status display module is used to display the frequency, duty cycle and For the remaining stimulation time, the operation buttons include a start stimulation button, a stop stimulation button and a switch key; the switch key is used to control the opening and closing of the stimulation channel, the start stimulation button is used to send an instruction to start stimulation, and the stop stimulation button is used to send a stop stimulation button instructions.

为了实现上述目的，本发明还提供一种多通道无线光遗传刺激方法，包 括：In order to achieve the above object, the present invention also provides a multi-channel wireless optogenetic stimulation method, comprising:

设置阵列式刺激光极，包括多个用于发出光线的区域；Provide an array of stimulating light poles, including multiple areas for emitting light;

设置光刺激参数，所述光刺激参数包括脉冲的频率、占空比、刺激时间 和一个或多个刺激通道；Light stimulation parameters are set, and the light stimulation parameters include pulse frequency, duty cycle, stimulation time and one or more stimulation channels;

通过光刺激参数生成电脉冲信号给光刺激参数中刺激通道电连通的阵列 式刺激光极的区域。The electrical pulse signal is generated through the light stimulation parameter to stimulate the area of the array type stimulating the light pole electrically connected to the stimulation channel in the light stimulation parameter.

本发明所述多通道无线光遗传刺激系统及方法采用阵列方式进行光刺 激，拥有更大面积的刺激范围，更加适用于细胞培养及大面积光照需求的应 用场所；阵列各个通道可以单独控制其开闭，适用于多区域的选择性刺激的 研究；可以提供多种不同刺激参数的设置，为光遗传技术的应用提供了一种 可靠的工具；采用无线通信技术，并配上了功率增强及高增益天线，拥有更 远的传输距离，传输信号更加稳定。The multi-channel wireless optogenetic stimulation system and method of the present invention adopts an array mode for light stimulation, has a larger area of stimulation range, and is more suitable for cell culture and application sites requiring large-area illumination; each channel of the array can be independently controlled. It is suitable for the research of selective stimulation of multiple regions; it can provide a variety of different stimulation parameter settings, providing a reliable tool for the application of optogenetic technology; it adopts wireless communication technology, and is equipped with power enhancement and high The booster antenna has a longer transmission distance and a more stable transmission signal.

为了实现上述以及相关目的，本发明的一个或多个方面包括后面将详细 说明特别指出的特征。下面的说明以及附图详细说明了本发明的某些示例性 方面。然而，这些方面指示的仅仅是可使用本发明的原理的各种方式中的一 些方式。此外，本发明旨在包括所有这些方面以及它们的等同物。To the accomplishment of the foregoing and related ends, one or more aspects of the invention include the features particularly pointed out in the hereinafter detailed description. The following description and accompanying drawings detail certain exemplary aspects of the invention. These aspects are indicative, however, of but a few of the various ways in which the principles of the invention may be employed. Furthermore, the invention is intended to include all such aspects and their equivalents.

附图说明Description of drawings

图1是本发明所述多通道无线光遗传刺激系统的结构示意图；Fig. 1 is a schematic structural view of the multi-channel wireless optogenetic stimulation system of the present invention;

图2是本发明所述上位机的示意图；Fig. 2 is the schematic diagram of upper computer described in the present invention;

图3是本发明中所述阵列式刺激光极底板结构示意图。Fig. 3 is a schematic diagram of the structure of the array stimulating light electrode base plate in the present invention.

本发明目的的实现、功能特点及优点将结合实施例，参照附图做进一步 说明。The realization, functional characteristics and advantages of the present invention will be further described with reference to the accompanying drawings in conjunction with the embodiments.

具体实施方式Detailed ways

以下将结合附图对本发明的具体实施例进行详细描述。Specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

图1是本发明所述多通道无线光遗传刺激系统的结构示意图，如图1所 示，所述多通道无线光遗传刺激系统包括上位机100、光刺激器400以及阵列 式刺激光极500，所述光刺激器400包括多个刺激通道，不同刺激通道与阵列 式刺激光极500的不同区域电连通，所述上位机100用于设置光刺激参数， 所述光刺激参数包括脉冲的频率、占空比、刺激时间和一个或多个刺激通道， 所述上位机200和光刺激器400无线连接，所述光刺激器400接收上位机100 设置的光刺激参数，输出电脉冲信号给光刺激参数中刺激通道电连通的阵列 式刺激光极500的区域。Fig. 1 is a schematic structural diagram of the multi-channel wireless optogenetic stimulation system of the present invention. As shown in Fig. 1, the multi-channel wireless optogenetic stimulation system includes ahost computer 100, aphotostimulator 400, and anarray stimulation optode 500, Theoptical stimulator 400 includes a plurality of stimulation channels, and different stimulation channels are electrically connected to different areas of the arraystimulation light pole 500. Thehost computer 100 is used to set optical stimulation parameters, and the optical stimulation parameters include pulse frequency, Duty cycle, stimulation time and one or more stimulation channels, thehost computer 200 is wirelessly connected to theoptical stimulator 400, theoptical stimulator 400 receives the optical stimulation parameters set by thehost computer 100, and outputs electrical pulse signals to the optical stimulation parameters The region of the arrayed stimulation optode 500 in electrical communication with the stimulation channel.

在一个实施例中，多通道无线光遗传刺激系统还包括无线发送模块200 和无线接收模块300，所述无线发送模块连接上位机和无线接收模块，所述无 线接收模块连接无线发送模块和光刺激器，所述无线发送模块将上位机的光 刺激参数通过无线接收模块发送给光刺激器。In one embodiment, the multi-channel wireless optogenetic stimulation system also includes awireless sending module 200 and awireless receiving module 300, the wireless sending module is connected to the host computer and the wireless receiving module, and the wireless receiving module is connected to the wireless sending module and the optical stimulator , the wireless sending module sends the optical stimulation parameters of the upper computer to the optical stimulator through the wireless receiving module.

可选地，所述无线发送模块200包括射频放大205以及高增益天线204。Optionally, thewireless sending module 200 includes a radio frequency amplifier 205 and a high-gain antenna 204 .

在一个实施例中，所述阵列式刺激光极500包括光极底板502以及多个 光极501，用于发出光线对神经细胞进行光刺激。In one embodiment, thearray stimulation optode 500 includes anoptode base plate 502 and a plurality ofoptodes 501 for emitting light to stimulate nerve cells.

可选地，所述光极底板基底材料采用硅基底，且表面镀有一层环氧树脂 薄膜。Optionally, the base material of the optode base plate is a silicon base, and the surface is coated with a layer of epoxy resin film.

可选地，所述光极为μLED。Optionally, the light poles are μLEDs.

可选地，所述阵列式刺激光极包括24个光极，光刺激器包括6个光刺激 通道，每四个光极为一个区域，每一个区域通过一个光刺激通道控制。Optionally, the array stimulation optode includes 24 optodes, and the optical stimulator includes 6 optical stimulation channels, every four optical poles is an area, and each area is controlled by an optical stimulation channel.

在一个实施例中，所述光刺激器400包括电源驱动电路401、开关402、 时钟电路403、指示灯404、无线通信接口405、第二微控制器406、复位电 路407以及输出电路408，电源驱动电路用于供电，开关用于光刺激器的开启 与关闭，时钟电路用于为第二微控制器提供时钟信号，指示灯包括电源指示 灯以及通信状态指示灯，电源指示灯用于显示光刺激器的开启与关闭状态， 亮则表示光刺激器在工作，通信状态指示灯用于显示光刺激器与上位机的通 信状态，当光刺激器成功接收到上位机发送来的信号后，通信状态指示灯将 发生亮暗变化，无线通信接口用于连接上位机与光刺激器，第二微控制器用 于提取光刺激参数，经过处理后，以电脉冲信号形式传递给输出电路，输出 电路用于连接光刺激器与阵列式刺激光极，将第二微控制器输出的电脉冲信 号传递给光极，复位电路用于将光刺激器恢复到初始化状态。In one embodiment, theoptical stimulator 400 includes apower drive circuit 401, aswitch 402, aclock circuit 403, anindicator light 404, awireless communication interface 405, asecond microcontroller 406, areset circuit 407, and anoutput circuit 408. The driving circuit is used for power supply, the switch is used for turning on and off the light stimulator, the clock circuit is used for providing a clock signal for the second microcontroller, the indicator light includes a power indicator light and a communication status indicator light, and the power indicator light is used to display light The on and off status of the stimulator, if it is on, it means that the optical stimulator is working. The communication status indicator is used to display the communication status between the optical stimulator and the host computer. When the optical stimulator successfully receives the signal sent by the host computer, the communication The status indicator light will change from bright to dark. The wireless communication interface is used to connect the upper computer and the optical stimulator. The second microcontroller is used to extract the optical stimulation parameters. After processing, it is transmitted to the output circuit in the form of electrical pulse signals. In order to connect the light stimulator and the array stimulation light pole, the electrical pulse signal output by the second micro-controller is transmitted to the light pole, and the reset circuit is used to restore the light stimulator to the initialization state.

在一个实施例中，所述上位机包括参数设置模块、状态显示模块和操作 按钮，所述参数设置模块用于设置光刺激参数，所述状态显示模块用于显示 当前光刺激的频率、占空比以及剩余刺激时间，所述操作按钮包括开始刺激 按钮、停止刺激按钮以及开关键；开关键用于控制刺激通道的启闭，开始刺 激按钮用于发送开始刺激的指令，停止刺激按钮用于发送停止刺激的指令。In one embodiment, the host computer includes a parameter setting module, a status display module and operation buttons, the parameter setting module is used to set the optical stimulation parameters, and the status display module is used to display the current frequency of optical stimulation, duty cycle Ratio and remaining stimulation time, the operation buttons include a start stimulation button, a stop stimulation button and a switch key; the switch key is used to control the opening and closing of the stimulation channel, the start stimulation button is used to send an instruction to start stimulation, and the stop stimulation button is used to send Instructions to stop stimulation.

在一个实施例中，多通道无线光遗传刺激系统，包括上位机、无线发送 模块、无线接收模块、光刺激器以及阵列式刺激光极，其中：In one embodiment, the multi-channel wireless optogenetic stimulation system includes a host computer, a wireless sending module, a wireless receiving module, a light stimulator and an array-type stimulating optode, wherein:

所述上位机通过USB接口与无线发送模块连接，所述无线接收模块与光 刺激器的无线通信接口相连接，阵列式刺激光极与光刺激器的输出电路连接；The host computer is connected with the wireless sending module through the USB interface, the wireless receiving module is connected with the wireless communication interface of the optical stimulator, and the array stimulates the light electrode and the output circuit of the optical stimulator is connected;

所述上位机用于设置光刺激参数，包括参数设置模块、状态显示模块以 及操作按钮；Described upper computer is used for setting photostimulation parameter, comprises parameter setting module, status display module and operation button;

所述无线发送模块用于将上位机设置的光刺激参数通过2.4G无线传输的 方式发送给光刺激器；The wireless sending module is used to send the optical stimulation parameters set by the host computer to the optical stimulator by means of 2.4G wireless transmission;

所述无线接收模块用于接收来自无线发送模块的信号并将其传输给光刺 激器；The wireless receiving module is used to receive the signal from the wireless sending module and transmit it to the optical stimulator;

所述光刺激器用于将来自上位机的刺激信息进行处理，然后输出相应的 电脉冲信号给阵列式刺激光极；The optical stimulator is used to process the stimulation information from the host computer, and then output the corresponding electrical pulse signal to the array stimulation light pole;

所述阵列式刺激光极包括光极底板以及24个μLED芯片，用于发出光线 对神经细胞进行光刺激。The array stimulation optode includes an optode base plate and 24 μLED chips, which are used to emit light to stimulate nerve cells.

可选地，所述光刺激参数频率的调节范围为1-500Hz，占空比的调节范围 为0-100％，可调控的刺激通道数为6个，每个刺激通道可以通过上位机独立 控制每个刺激通道的开启与关闭，从而达到对神经细胞区域选择性刺激的目 的。Optionally, the adjustment range of the light stimulation parameter frequency is 1-500Hz, the adjustment range of the duty cycle is 0-100%, the number of adjustable stimulation channels is 6, and each stimulation channel can be independently controlled by the host computer The opening and closing of each stimulation channel achieves the purpose of selectively stimulating the nerve cell area.

可选地，所述光刺激器集成有电源驱动电路、开关、时钟电路、指示灯、 无线通信接口、第二微控制器、复位电路以及输出电路。所述各模块通过PCB 板上的导线相互连接，电源驱动电路用于给刺激器及无线通信模块供电，包 括3.7V锂电池及RT9193-33GB低压差线性稳压器。开关用于控制刺激器的 开启与关闭。时钟电路包括8M有源晶振以及去耦电容，用于为第二微控制器 提供时钟信号。指示灯包括电源指示灯以及通信状态指示灯，电源指示灯用 于显示刺激器的开启与关闭状态，亮则表示刺激器在工作，通信状态指示灯用于显示刺激器与上位机的通信状态，当刺激器成功接收到上位机发送来的 信号后，通信状态指示灯将发生亮暗变化。无线通信接口用于连接无线接收 模块与刺激器。第二微控制器用于提取无线接收模块接收到的无线指令信息， 经过处理后，以电脉冲信号形式传递给输出电路。复位电路包括复位开关、 电容以及电阻，用于将刺激器恢复到初始化状态。输出电路用于连接刺激器 与光极，将第二微控制器输出的电脉冲信号传递给光极。Optionally, the optical stimulator is integrated with a power drive circuit, a switch, a clock circuit, an indicator light, a wireless communication interface, a second microcontroller, a reset circuit and an output circuit. The modules are connected to each other through wires on the PCB board, and the power drive circuit is used to supply power to the stimulator and the wireless communication module, including a 3.7V lithium battery and an RT9193-33GB low-dropout linear regulator. The switch is used to control the opening and closing of the stimulator. The clock circuit includes an 8M active crystal oscillator and a decoupling capacitor for providing a clock signal for the second microcontroller. The indicator light includes a power indicator light and a communication status indicator light. The power indicator light is used to display the on and off status of the stimulator. When it is on, it means that the stimulator is working. The communication status indicator light is used to display the communication status between the stimulator and the host computer. When the stimulator successfully receives the signal from the host computer, the communication status indicator light will change from bright to dark. The wireless communication interface is used to connect the wireless receiving module and the stimulator. The second micro-controller is used to extract the wireless instruction information received by the wireless receiving module, and after processing, transmit it to the output circuit in the form of electric pulse signal. The reset circuit includes a reset switch, a capacitor and a resistor for restoring the stimulator to an initialization state. The output circuit is used to connect the stimulator and the light pole, and transmit the electric pulse signal output by the second micro-controller to the light pole.

所述光刺激器的顶端设置有3×3mm的挖孔区域，挖孔区域两侧设置有6 对矩形焊盘，用于与阵列式刺激光极连接。A 3×3mm hole-digging area is provided on the top of the optical stimulator, and 6 pairs of rectangular pads are provided on both sides of the hole-digging area for connecting with the array stimulating light electrodes.

所述光极底板的两侧设置有6对矩形镀铜焊盘，每对焊盘通过镀铜导线 与4对μLED焊盘连接。There are 6 pairs of rectangular copper-plated pads arranged on both sides of the optical electrode base plate, and each pair of pads is connected to 4 pairs of μLED pads through copper-plated wires.

6对矩形镀铜焊盘一侧设置为正极，另外一侧设置为负极，其尺寸为 500μm×300μm。One side of the 6 pairs of rectangular copper-plated pads is set as the positive pole, and the other side is set as the negative pole, with a size of 500 μm×300 μm.

所述μLED焊盘的形状为圆形，其直径为70μm。The μLED bonding pad is circular in shape with a diameter of 70 μm.

所述光极底板基底材料采用硅基底，且表面镀有一层环氧树脂薄膜。The base material of the optical electrode bottom plate is silicon base, and the surface is coated with a layer of epoxy resin film.

所述μLED的波长为470nm。The wavelength of the μLED is 470nm.

光刺激器400通过无线接收模块300接收来自上位机100通过无线发送 模块200发送的刺激命令，然后将处理过的刺激信号传输给阵列式刺激光极 500，完成对神经细胞的光刺激。Theoptical stimulator 400 receives the stimulation command sent from thehost computer 100 through thewireless transmission module 200 through thewireless receiving module 300, and then transmits the processed stimulation signal to thearray stimulation optode 500 to complete the optical stimulation of the nerve cells.

所述上位机100是在PC上运行的控制程序，可供用户对光刺激的频率、 占空比以及阵列通道的开关进行调整。Thehost computer 100 is a control program running on a PC, which can be used by the user to adjust the frequency, duty cycle and switch of the array channel for optical stimulation.

所述无线发送模块200由USB转串口接头201、USB转串口芯片202、 第一微控制器203、高增益天线204、射频放大205、无线芯片206以及LDO 稳压芯片207构成。无线发送模块200通过USB转串口接头201与上位机100 进行连接，实现刺激信号的通信。LDO稳压芯片207将USB转串口接头201 提供的电压进行稳压后为第一微控制器203以及无线芯片206提供电源。USB 转串口芯片202将来自上位机的光刺激指令转换后传递给第一微控制器203， 第一微控制器203将接收到来自USB转串口芯片202的串行数据转化为数据 包传递给无线芯片206将指令发送至无线接收模块300，配合高增益天线204 及射频放大205可提升无线通信的距离以及信号传输的稳定性。Thewireless sending module 200 is composed of a USB-to-serial connector 201 , a USB-to-serial chip 202 , afirst microcontroller 203 , a high-gain antenna 204 , a radio frequency amplifier 205 , awireless chip 206 and an LDOvoltage regulator chip 207 . Thewireless sending module 200 is connected with thehost computer 100 through the USB-to-serial port connector 201 to realize the communication of stimulation signals. TheLDO regulator chip 207 stabilizes the voltage provided by the USB-to-serial connector 201 to provide power for thefirst microcontroller 203 and thewireless chip 206 . The USB-to-serial port chip 202 converts the optical stimulation command from the host computer and transmits it to thefirst microcontroller 203, and thefirst microcontroller 203 converts the serial data received from the USB-to-serial port chip 202 into data packets and transmits them to the wireless Thechip 206 sends the instruction to thewireless receiving module 300, and cooperates with the high-gain antenna 204 and the radio frequency amplifier 205 to improve the distance of wireless communication and the stability of signal transmission.

所述无线接收模块包括2×4排针接头301、无线芯片302、以及天线303。 当无线接收模块300接收到来自无线发送模块200发送的刺激指令后，将通 过2×4排针接头301把接收到的指令信息传递给刺激器400。The wireless receiving module includes a 2×4pin header 301 , awireless chip 302 , and anantenna 303 . When thewireless receiving module 300 receives the stimulation instruction from thewireless sending module 200, it will transmit the received instruction information to thestimulator 400 through the 2×4pin header 301.

刺激器400包括电源401、开关402、时钟电路403、指示灯404、无线 通信接口405、第二微控制器406、复位电路407以及输出电路408。电源401 为第二微控制器406以及无线接收模块300供电。当第二微控制器406通过 无线通信接口405接收到来自无线接收模块300的刺激指令后，第二微控制 器406首先将校验接收到的指令信息是否正确，若正确则提取指令中的刺激 信息，若错误则继续等待接收新的指令信息，提取的刺激信息包含开始刺激 指令或停止刺激指令，其中开始刺激指令还包括开启的通道、刺激频率以及 刺激占空比，第二微控制器406将根据指令信息配置通道的输出电脉冲，然 后通过输出电路传递给光极完成刺激信号输出的更新。Thestimulator 400 includes apower supply 401, aswitch 402, aclock circuit 403, anindicator light 404, awireless communication interface 405, asecond microcontroller 406, areset circuit 407 and anoutput circuit 408. Thepower supply 401 supplies power to thesecond microcontroller 406 and thewireless receiving module 300 . After thesecond microcontroller 406 receives the stimulation instruction from thewireless receiving module 300 through thewireless communication interface 405, thesecond microcontroller 406 will first check whether the received instruction information is correct, and if it is correct, extract the stimulus in the instruction. Information, if it is wrong, continue to wait for receiving new instruction information, the extracted stimulation information includes start stimulation instruction or stop stimulation instruction, wherein the start stimulation instruction also includes the opened channel, stimulation frequency and stimulation duty cycle, thesecond microcontroller 406 The output electrical pulse of the channel will be configured according to the instruction information, and then transmitted to the optode through the output circuit to complete the update of the stimulation signal output.

阵列式刺激光极500由μLED501及光极底板502构成。光极底板502与 输出电路408连接，将来自光刺激器400的输出电信号通过铜导线传递给 μLED501，从而完成对神经细胞的光刺激。The array stimulatinglight pole 500 is composed of μLED501 and lightpole bottom plate 502 . The opticalelectrode base plate 502 is connected with theoutput circuit 408, and transmits the output electrical signal from theoptical stimulator 400 to theµLED 501 through the copper wire, thereby completing the optical stimulation of the nerve cells.

如图2所示，上位机100包括参数设置模块、状态显示模块104和操作 按钮，参数设置模块包括刺激时间设置模块101、刺激模式配置模块102、无 线通信参数配置模块103和以及刺激参数设置模块107；操作按钮包括通道选 择开关按钮105和开始结束按钮106。刺激时间设置模块101用于设置刺激时 间长短。刺激模式配置模块102用于刺激模式的选择，包括自由刺激模式与 定时模式，自由刺激模式为用户通过开始刺激与结束刺激按钮自由决定刺激 时长，定时模式为光刺激器进行用户设置时长的光刺激，刺激模式可供选择， 且操控界面简洁。无线通信参数配置模块103用于配置无线发送模块200的 相关参数，包括端口选择、波特率设置、数据位长度以及校验位设置。无线 通信参数配置模块103用于显示当前刺激参数以及定时模式下的刺激剩余时 间。通道选择开关按钮105为光刺激阵列的开关，用户可通过在上位机100 上的通道选择开关按钮105选择不同阵列的开关，从而达到不同区域选择性 刺激的目的。开始结束按钮106用于控制光刺激的开始与结束，点击开始刺激按钮即开始光刺激，点击结束刺激即停止光刺激。刺激参数设置模块107 包括刺激频率与占空比的修改，用户可直接通过在框中输入数值对刺激参数 进行修改。As shown in Figure 2, theupper computer 100 includes a parameter setting module, astatus display module 104 and operation buttons, and the parameter setting module includes a stimulationtime setting module 101, a stimulationmode configuration module 102, a wireless communicationparameter configuration module 103 and a stimulation parameter setting module 107 : The operation buttons include the channelselection switch button 105 and the start and endbuttons 106 . Stimulationtime setting module 101 is used for setting the length of stimulation time. The stimulationmode configuration module 102 is used for the selection of stimulation modes, including free stimulation mode and timing mode. The free stimulation mode is for the user to freely determine the stimulation duration through the start stimulation and end stimulation buttons, and the timing mode is for the optical stimulator to perform optical stimulation with a user-set duration , Stimulation modes are available for selection, and the control interface is simple. The wireless communicationparameter configuration module 103 is used to configure the relevant parameters of thewireless transmission module 200, including port selection, baud rate setting, data bit length and check bit setting. The wireless communicationparameter configuration module 103 is used for displaying the stimulation remaining time under current stimulation parameter and timing mode. The channelselection switch button 105 is the switch of the optical stimulation array, and the user can select switches of different arrays through the channelselection switch button 105 on thehost computer 100, so as to achieve the purpose of selective stimulation in different regions. The start andend button 106 is used to control the start and end of the light stimulation, click the start stimulation button to start the light stimulation, and click the end stimulation to stop the light stimulation. The stimulationparameter setting module 107 includes modification of the stimulation frequency and duty cycle, and the user can directly modify the stimulation parameters by inputting values in the boxes.

如图3所示，光极底板502由矩形焊盘5021、μLED焊盘5022以及镀铜 导线5023构成。所述矩形焊盘5021分布在光极底板502的两侧，且一边为 正极，另外一边为负极。μLED焊盘5022每四个为一组与矩形焊盘5021通过 镀铜导线5023彼此连接，μLED的正极与正极矩形焊盘相连的LED焊盘连接， 每四个μLED共用一个正极与负极矩形焊盘形成一个4×1的μLED阵列，6 组正负焊盘组成一个1×6的阵列，每组单独与光刺激器400的输出口相连， 通过光刺激器400的第二微控制器406控制每个阵列的开关即可实现对较大 区域神经细胞的选择性刺激。As shown in FIG. 3 , the opticalelectrode base plate 502 is composed of arectangular pad 5021 , aµLED pad 5022 and a copper-platedwire 5023 . Therectangular pads 5021 are distributed on both sides of theoptode base plate 502, and one side is the positive pole, and the other side is the negative pole. Each group of fourμLED pads 5022 is connected to each other with therectangular pads 5021 through copper-platedwires 5023, the anode of the μLED is connected to the LED pad connected to the positive rectangular pad, and each four μLEDs share a positive and negative rectangular pad A 4×1 μLED array is formed, and 6 groups of positive and negative pads form a 1×6 array, and each group is connected to the output port of theoptical stimulator 400 independently, and each group is controlled by thesecond microcontroller 406 of theoptical stimulator 400. An array of switches can realize the selective stimulation of nerve cells in a large area.

本发明中的无线芯片可采用NRF24L01+,该芯片工作频段为 2.4GHz～2.5GHz，数据传输速率最快可达2Mbps，并且可直接通过SPI接口与 第一微控制器203相连接。The wireless chip in the present invention can use NRF24L01+, the working frequency band of this chip is 2.4GHz～2.5GHz, the fastest data transmission rate can reach 2Mbps, and can be directly connected with thefirst microcontroller 203 through the SPI interface.

本发明中光刺激器的第二微控制器采用STM32F103RCT6。The second microcontroller of the light stimulator in the present invention adopts STM32F103RCT6.

本发明中光刺激器的电源采用3.7V锂电池供电。The power supply of the light stimulator in the present invention is powered by a 3.7V lithium battery.

本发明中光刺激频率的可调节范围为1-500Hz，占空比设置范围为 0-100％。In the present invention, the adjustable range of the light stimulation frequency is 1-500 Hz, and the setting range of the duty ratio is 0-100%.

本发明采用的μLED波长为470nm。The μLED used in the present invention has a wavelength of 470nm.

多通道无线光遗传刺激系统各构成装置结构简单，成本较低，易于生产。Each component device of the multi-channel wireless optogenetic stimulation system has simple structure, low cost and easy production.

本发明还提供一种多通道无线光遗传刺激方法，包括：The present invention also provides a multi-channel wireless optogenetic stimulation method, comprising:

设置阵列式刺激光极，包括多个用于发出光线的区域；Provide an array of stimulating light poles, including multiple areas for emitting light;

设置光刺激参数，所述光刺激参数包括脉冲的频率、占空比、刺激时间 和一个或多个刺激通道；Light stimulation parameters are set, and the light stimulation parameters include pulse frequency, duty cycle, stimulation time and one or more stimulation channels;

通过光刺激参数生成电脉冲信号给光刺激参数中刺激通道电连通的阵列 式刺激光极的区域。The electrical pulse signal is generated through the light stimulation parameter to stimulate the area of the array type stimulating the light pole electrically connected to the stimulation channel in the light stimulation parameter.

在一个实施例中，所述第二微控制器构建神经网络结构模型，所述神经 网络结构模型的输入是脑CT的图像特征(所述图像特征包括细胞状态及坐标， 所述细胞状态包括细胞损伤、坏死、凋亡等，还可以包括不同细胞状态占总 细胞的比例)，输出是光刺激参数，采集细胞激活效果达到要求的多个客户端 的脑CT和光刺激参数对神经网络结构模型进行训练，第二微控制器通过训练 后的神经网络结构模型获得新的脑CT的输出光刺激参数作为参考传输给上位 机。In one embodiment, the second micro-controller constructs a neural network structure model, the input of the neural network structure model is the image features of brain CT (the image features include cell state and coordinates, and the cell state includes cell Injury, necrosis, apoptosis, etc., can also include the proportion of different cell states to the total cells), the output is the light stimulation parameters, and the brain CT and light stimulation parameters of multiple clients whose cell activation effect meets the requirements are collected to train the neural network structure model , the second micro-controller obtains new brain CT output light stimulation parameters through the trained neural network structure model as a reference and transmits them to the host computer.

可选地，第二微控制器根据细胞膜外的阳离子内流确定细胞激活效果是 否达到要求，通过训练后的神经网络结构模型可以获得激活区域或/和不通过 激活区域的脉冲的频率、占空比、刺激时间等。Optionally, the second microcontroller determines whether the cell activation effect meets the requirements according to the cation influx outside the cell membrane, and the frequency and duty of the pulses in the activation area or/and not passing through the activation area can be obtained through the trained neural network structure model Ratio, stimulation time, etc.

在一个实施例中，利用多通道无线光遗传刺激系统进行光刺激的方法包 括：In one embodiment, the method for performing photostimulation using a multi-channel wireless optogenetic stimulation system includes:

将无线接收模块按标识方向插入到刺激器上，打开刺激器上开关，此时 光刺激频率为0，占空比为0％，无刺激信号；Insert the wireless receiving module into the stimulator according to the marked direction, and turn on the switch on the stimulator. At this time, the light stimulation frequency is 0, the duty cycle is 0%, and there is no stimulation signal;

将无线发送模块插入到电脑USB端口，打开上位机软件，进行无线模块 参数配置；Insert the wireless sending module into the USB port of the computer, open the host computer software, and configure the parameters of the wireless module;

选择刺激模式，所述光刺激系统提供两种刺激模式，一种为自由刺激模 式，由用户自己通过点击开始刺激以及结束刺激按钮自由决定刺激时间，另 一种为定时刺激模式，即根据用户设置时间产生所设置时长的光刺激；Select the stimulation mode, the optical stimulation system provides two stimulation modes, one is the free stimulation mode, the user can freely decide the stimulation time by clicking the start stimulation and end stimulation buttons, and the other is the timing stimulation mode, that is, according to the user setting Time to generate light stimulation of the set duration;

选择要打开的刺激通道，打开对应刺激通道的开关即代表该刺激通道刺 激打开；Select the stimulation channel to be opened, and turning on the switch of the corresponding stimulation channel means that the stimulation channel is stimulated to open;

配置刺激参数，用户可通过在上位机上输入频率、占空比等刺激参数来 进行参数配置；Configure stimulation parameters, users can configure parameters by inputting stimulation parameters such as frequency and duty cycle on the host computer;

点击开始刺激进行刺激参数的无线发送，光刺激器将在接收到配置参数 后开始相应的光刺激。若需修改参数，只需在上位机界面输入修改的刺激参 数后，点击开始刺激即可完成刺激参数的修改；Click Start Stimulation to wirelessly send stimulation parameters, and the optical stimulator will start corresponding optical stimulation after receiving the configuration parameters. If you need to modify the parameters, you only need to input the modified stimulation parameters on the host computer interface, and then click Start Stimulation to complete the modification of the stimulation parameters;

刺激结束后关闭刺激器开关，取下无线接收模块及无线发送模块。After the stimulation is over, turn off the stimulator switch, and remove the wireless receiving module and the wireless sending module.

在一个实施例中，上位机通过无线发送模块将设置好的刺激参数发送给 与无线接收模块连接的光刺激器，光刺激器接收到刺激指令后，经过第二微 处理器处理后输出对应的电脉冲信号，电脉冲信号经过输出电路传递给阵列 式刺激光极，完成对神经细胞的刺激。用户可根据调控需求在上位机上通过 选择多通道的开启或关闭实现不同区域的选择性刺激，在频率及占空比输入 窗口修改数值实现对光刺激参数的修改，修改的刺激信息将通过2.4G无线传 输的方式传递给刺激器，从而完成对刺激器实时的修改。In one embodiment, the upper computer sends the set stimulation parameters to the optical stimulator connected to the wireless receiving module through the wireless sending module. After receiving the stimulation instruction, the optical stimulator outputs corresponding Electrical pulse signal, the electrical pulse signal is transmitted to the array stimulation light electrode through the output circuit to complete the stimulation of nerve cells. The user can realize the selective stimulation of different areas by selecting the multi-channel on or off on the host computer according to the control requirements, modify the value in the frequency and duty cycle input window to modify the light stimulation parameters, and the modified stimulation information will be passed through 2.4G The way of wireless transmission is transmitted to the stimulator, so as to complete the modification of the stimulator in real time.

在一个实施例中，利用多通道无线光遗传刺激系统进行光刺激的方法还 包括：In one embodiment, the method for performing photostimulation using a multi-channel wireless optogenetic stimulation system also includes:

构建神经网络结构模型，所述神经网络结构模型的输入是脑CT的图像特 征(所述图像特征包括细胞状态及坐标，所述细胞状态包括细胞损伤、坏死、 凋亡等，还可以包括不同细胞状态占总细胞的比例)，输出是光刺激参数；Build a neural network structure model, the input of the neural network structure model is the image feature of brain CT (the image feature includes cell state and coordinates, and the cell state includes cell damage, necrosis, apoptosis, etc., and can also include different cell The state accounts for the proportion of the total cells), and the output is the photostimulation parameter;

采集细胞激活效果达到要求的多个客户端的脑CT和光刺激参数对神经网 络结构模型进行训练；Collect the brain CT and light stimulation parameters of multiple clients whose cell activation effect meets the requirements to train the neural network structure model;

通过训练后的神经网络结构模型获得新的脑CT的输出光刺激参数。The output light stimulation parameters of the new brain CT are obtained through the trained neural network structure model.

可选地，包括：根据细胞膜外的阳离子内流确定细胞激活效果是否达到 要求，通过训练后的神经网络结构模型可以获得激活区域或/和不通过激活区 域的脉冲的频率、占空比、刺激时间等。Optionally, it includes: determining whether the cell activation effect meets the requirements according to the influx of cations outside the cell membrane, and the frequency, duty cycle, and stimulation of pulses in the activation area or/and not passing through the activation area can be obtained through the trained neural network structure model. time etc.

本发明的可独立调控的多通道无线光遗传刺激系统使用时，上位机将设 置好的刺激参数通过无线发送模块发送至与无线接收模块相连的光刺激模 块，经过微处理器处理后输出相应的电信号给阵列式刺激光极，从而使μLED 输出具有一定频率、占空比的光刺激信号。通过上位机的设置可以实时完成 刺激信号的修改与刺激区域的选择，从而完成对大面积神经细胞的区域选择 性刺激。本发明设计的可独立调控的多通道无线光遗传刺激系统将为光遗传 在体外神经作用机制的研究提供有效的支持，促进今后光遗传的应用与发展。When the independently adjustable multi-channel wireless optogenetic stimulation system of the present invention is used, the upper computer sends the set stimulation parameters to the optical stimulation module connected to the wireless receiving module through the wireless sending module, and outputs the corresponding output after being processed by the microprocessor. The electrical signal is used to stimulate the light pole array, so that the μLED outputs a light stimulation signal with a certain frequency and duty cycle. The modification of the stimulation signal and the selection of the stimulation area can be completed in real time through the setting of the host computer, so as to complete the regional selective stimulation of a large area of nerve cells. The independently adjustable multi-channel wireless optogenetic stimulation system designed by the present invention will provide effective support for the research on the mechanism of optogenetics in vitro, and promote the application and development of optogenetics in the future.

以上实施例仅用以说明本发明的技术方案而非限制，尽管参照较佳实施 例对本发明进行了详细说明，本领域的普通技术人员应当理解，可以对本发 明的技术方案进行修改或等同替换，而不脱离本发明技术方案的精神和范围。The above embodiments are only used to illustrate the technical solutions of the present invention without limitation. Although the present invention has been described in detail with reference to preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced. Without departing from the spirit and scope of the technical solution of the present invention.

Claims (9)

1. A multi-channel wireless optogenetic stimulation system is characterized by comprising an upper computer, an optical stimulator and an array type stimulation optode, wherein the optical stimulator comprises a plurality of stimulation channels, different stimulation channels are electrically communicated with different areas of the array type stimulation optode, the upper computer is used for setting optical stimulation parameters, the optical stimulation parameters comprise pulse frequency, duty ratio, stimulation time and one or more stimulation channels, the upper computer is in wireless connection with the optical stimulator, the optical stimulator receives the optical stimulation parameters set by the upper computer and outputs electric pulse signals to the areas of the array type stimulation optode, which are electrically communicated with the stimulation channels, in the optical stimulation parameters,

the light is a mu LED and,

each four light poles in the array type laser-emitting pole are areas, each area is controlled by a light stimulation channel, the light pole base plate is composed of rectangular bonding pads, mu LED bonding pads and copper-plated wires, the rectangular bonding pads are distributed on two sides of the light pole base plate, one side of each rectangular bonding pad is a positive electrode, the other side of each rectangular bonding pad is a negative electrode, and each four mu LEDs share one positive electrode and each negative electrode rectangular bonding pad to form a 4 multiplied by 1 mu LED array;

the optical stimulator comprises a second microcontroller, the second microcontroller is used for extracting optical stimulation parameters, the optical stimulation parameters are transmitted to an output circuit in the form of electric pulse signals after being processed, the output circuit is used for connecting the optical stimulator and an array type stimulation optode, the electric pulse signals output by the second microcontroller are transmitted to the optode,

the second microcontroller builds a neural network structure model, the input of the neural network structure model is the image characteristic of brain CT, the output is the optical stimulation parameter, the brain CT and the optical stimulation parameters of a plurality of clients with the cell activation effect reaching the requirement are collected to train the neural network structure model, the second microcontroller obtains the output optical stimulation parameter of the new brain CT through the trained neural network structure model and transmits the output optical stimulation parameter to the upper computer as a reference,

and the second microcontroller determines whether the cell activation effect meets the requirement according to the cation inflow outside the cell membrane, and the trained neural network structure model is used for obtaining the frequency, the duty ratio and the stimulation time of the pulse of the activation region or/and the pulse which does not pass through the activation region.

2. The multi-channel wireless optogenetic stimulation system of claim 1, further comprising a wireless transmitting module and a wireless receiving module, wherein the wireless transmitting module is connected with the upper computer and the wireless receiving module, the wireless receiving module is connected with the wireless transmitting module and the optical stimulator, and the wireless transmitting module transmits the optical stimulation parameters of the upper computer to the optical stimulator through the wireless receiving module.

3. The multi-channel wireless optogenetic stimulation system of claim 2 wherein the wireless transmission module comprises a radio frequency amplification and high gain antenna.

4. The multi-channel wireless optogenetic stimulation system of claim 1 wherein the array of optogenetic electrodes comprises an optobase plate and a plurality of optogenetic electrodes for emitting light to optically stimulate the nerve cells.

5. The multi-channel wireless optogenetic stimulation system of claim 4 wherein the optobase substrate material is a silicon substrate and the surface is coated with a thin film of epoxy.

6. The multi-channel wireless optogenetic stimulation system of claim 4 wherein the array of stimulating optodes comprises 24 optodes and the optical stimulator comprises 6 optical stimulation channels.

7. The multi-channel wireless optogenetic stimulation system of claim 1, wherein the optical stimulator comprises a power driving circuit, a switch, a clock circuit, an indicator light, a wireless communication interface, a second microcontroller, a reset circuit and an output circuit, wherein the power driving circuit is used for supplying power, the switch is used for switching on and off the optical stimulator, the clock circuit is used for providing clock signals for the second microcontroller, the indicator light comprises a power indicator light and a communication state indicator light, the power indicator light is used for displaying the on and off states of the optical stimulator, the light indicates that the optical stimulator is working, the communication state indicator light is used for displaying the communication state of the optical stimulator and the upper computer, the communication state indicator light changes in brightness after the optical stimulator successfully receives signals sent by the upper computer, the wireless communication interface is used for connecting the upper computer and the optical stimulator, and the reset circuit is used for recovering the optical stimulator to an initialized state.

8. The multi-channel wireless optogenetic stimulation system of claim 1, wherein the host computer comprises a parameter setting module for setting the optical stimulation parameters, a status display module for displaying the frequency, duty cycle and remaining stimulation time of the current optical stimulation, and an operation button comprising a start stimulation button, a stop stimulation button and a switch key; the on-off key is used for controlling the start and stop of the stimulation channel, the start stimulation button is used for sending a command for starting stimulation, and the stop stimulation button is used for sending a command for stopping stimulation.

9. A method of multi-channel wireless optogenetic stimulation comprising:

setting an array type stimulation light pole, wherein the array type stimulation light pole comprises a plurality of areas for emitting light, the light stimulator comprises a plurality of stimulation channels, and different stimulation channels are electrically communicated with different areas of the array type stimulation light pole;

setting optical stimulation parameters, wherein the optical stimulation parameters comprise the frequency, the duty cycle, the stimulation time and one or more stimulation channels of pulses;

generating an electric pulse signal to the area of the array-type stimulating laser electrode which is electrically communicated with the stimulating channel in the optical stimulation parameter through the optical stimulation parameter,

the light is a mu LED and,

each four light poles in the array type laser-emitting pole are areas, each area is controlled by a light stimulation channel, the light pole base plate is composed of rectangular bonding pads, mu LED bonding pads and copper-plated wires, the rectangular bonding pads are distributed on two sides of the light pole base plate, one side of each rectangular bonding pad is a positive electrode, the other side of each rectangular bonding pad is a negative electrode, and each four mu LEDs share one positive electrode and each negative electrode rectangular bonding pad to form a 4 multiplied by 1 mu LED array;

the optical stimulator comprises a second microcontroller, the second microcontroller is used for extracting optical stimulation parameters, the optical stimulation parameters are transmitted to an output circuit in the form of electric pulse signals after being processed, the output circuit is used for connecting the optical stimulator and an array type stimulation optode, the electric pulse signals output by the second microcontroller are transmitted to the optode, the reset circuit is used for recovering the optical stimulator to an initialized state,

the second microcontroller builds a neural network structure model, the input of the neural network structure model is the image characteristic of brain CT, the output is the optical stimulation parameter, the brain CT and the optical stimulation parameters of a plurality of clients with the cell activation effect reaching the requirement are collected to train the neural network structure model, the second microcontroller obtains the output optical stimulation parameter of the new brain CT through the trained neural network structure model and transmits the output optical stimulation parameter to the upper computer as a reference,

and the second microcontroller determines whether the cell activation effect meets the requirement according to the cation inflow outside the cell membrane, and the trained neural network structure model is used for obtaining the frequency, the duty ratio and the stimulation time of the pulse of the activation region or/and the pulse which does not pass through the activation region.

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