CN112934287A - Centrifugal tube and PRP extraction method using same - Google Patents

Abstract

The invention provides a centrifugal tube, wherein a cavity is arranged in the centrifugal tube, a first piston, a second piston and a flexible connecting pipe are arranged in the cavity, the cavity is sequentially divided into a first cavity, a second cavity and a third cavity by the first piston and the second piston, the cavity is provided with a first connecting port and a second connecting port which are communicated with the outside, the first piston is provided with a third connecting port, the flexible connecting pipe is positioned in the first cavity, one end of the flexible connecting pipe is connected with the first connecting port, the other end of the flexible connecting pipe is connected with the third connecting port, the flexible connecting pipe is used for communicating the second cavity with the outside, and the second connecting port is used for communicating the third cavity with the outside; the centrifugal tube is provided with a first plug and a second plug, the first plug is used for plugging the first connecting port, and the second plug is used for plugging the second connecting port.

Description

Centrifugal tube and PRP extraction method using same

Technical Field

The invention relates to the technical field of medical instruments, in particular to a centrifugal tube and a PRP extraction method using the centrifugal tube.

Background

The conventional separation method includes a centrifugation method in which an upper layer of plasma and platelets and a lower layer containing red blood cells are obtained by first centrifuging blood, a mixture of plasma and platelets is centrifuged for a second time to obtain an upper layer of a platelet poor plasma layer (PPP) and a lower layer of a platelet rich buffy coat (PRP), and the platelet rich buffy coat (PRP) is directly obtained.

The current centrifugation method adopts all be ordinary centrifuging tube, and two at least centrifuging tubes need be used in twice centrifugal operation, need place the unified planning of centrifuging tube that same blood sample corresponds moreover, and complex operation and increase storage difficulty consequently need a centrifuging tube of solving above-mentioned problem and the PRP extraction method of using this centrifuging tube.

Disclosure of Invention

The invention provides a centrifugal tube and a PRP extraction method using the centrifugal tube, and aims to solve the technical problems in the background art.

The invention provides a centrifugal tube, wherein a cavity is arranged in the centrifugal tube, a first piston, a second piston and a flexible connecting pipe are arranged in the cavity, the cavity is sequentially divided into a first cavity, a second cavity and a third cavity by the first piston and the second piston, the cavity is provided with a first connecting port and a second connecting port which are communicated with the outside, the first piston is provided with a third connecting port, the flexible connecting pipe is positioned in the first cavity, one end of the flexible connecting pipe is connected with the first connecting port, the other end of the flexible connecting pipe is connected with the third connecting port, the flexible connecting pipe is used for communicating the second cavity with the outside, and the second connecting port is used for communicating the third cavity with the outside;

the centrifugal tube is provided with a first plug and a second plug, the first plug is used for plugging the first connecting port, and the second plug is used for plugging the second connecting port.

Furthermore, the first connector is provided with a first elastic tubular body, and the first elastic tubular body is used for contacting and propping the inner wall of the flexible connecting pipe to be fixed.

Furthermore, the third connector is provided with a second elastic tubular body, and the second elastic tubular body is used for contacting and propping the inner wall of the flexible connecting pipe to be fixed.

Further, the second connector is provided with a bottom tube, the second piston is provided with a groove corresponding to the bottom tube, the groove is shaped correspondingly to the bottom tube, and the bottom tube penetrates through the tube wall of the centrifugal tube and the second piston.

Further, still include first sealing washer and second sealing washer, first sealing washer is located first piston with between the inner wall of centrifuging tube, the second sealing washer is located the second piston with between the inner wall of centrifuging tube.

Furthermore, the first plug comprises an outer layer made of a hard material and an inner layer made of an elastic material, a strip-shaped sealing opening is formed in one outward side of the inner layer, the strip-shaped sealing opening is sealed under the action of elastic force under the action of no external force, and the strip-shaped sealing opening is stretched under the action of external force to enable a syringe needle to penetrate through the strip-shaped sealing opening.

Furthermore, the second plug comprises an outer layer made of a hard material and an inner layer made of an elastic material, a strip-shaped sealing opening is formed in one outward side of the inner layer, the strip-shaped sealing opening is sealed under the action of elastic force under the action of no external force, and the strip-shaped sealing opening is stretched under the action of external force to enable a syringe needle to penetrate through the strip-shaped sealing opening.

Furthermore, the centrifuging tube is installed the one end department of second end cap is equipped with the base, the base is used for hiding the second end cap provides the supporting role.

The invention also provides a PRP extraction method using the centrifugal tube, which comprises the following steps:

injecting blood into the second cavity through the first port;

subjecting the centrifuge tube to a first centrifugation to separate an upper layer of platelet and plasma suspension and a lower layer of blood product comprising red blood cells;

extracting a mixture of platelets and plasma suspension through the first port;

injecting a mixture of platelets and plasma suspension into the third chamber through the second port;

centrifuging the centrifuge tube a second time to separate the mixed solution in the third chamber into an upper layer of a platelet poor plasma layer and a lower layer of a platelet rich buffy coat;

the platelet rich buffy coat is withdrawn through the second port.

Furthermore, the second connecting port is provided with a bottom tube, the second piston is provided with a groove corresponding to the bottom tube, the shape of the groove corresponds to that of the bottom tube, and the bottom tube penetrates through the tube wall of the centrifuge tube and the second piston;

after "centrifuging the centrifuge tube for the second time to separate the mixed solution in the third chamber into the upper layer of the platelet poor plasma layer and the lower layer of the platelet rich buffy coat", before "extracting the platelet rich buffy coat through the second connection port by a syringe" further includes:

drawing the platelet poor plasma layer through the second connector;

the centrifuge tube was inverted.

According to the centrifugal pump, the first piston and the second piston are arranged to form the first cavity, the second cavity and the third cavity, and the second cavity and the third cavity are respectively used as the first reaction cavity and the second reaction cavity, so that two times of centrifugal operation can be completed through the same centrifugal tube, and the storage difficulty and the operation difficulty are reduced.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

Drawings

Fig. 1 is a schematic view of an overall structure of a centrifugal tube according to an embodiment of the present invention.

Fig. 2 is a schematic cross-sectional view of the embodiment of fig. 1.

Fig. 3 is a flowchart illustrating steps of a PRP extraction method using the centrifugal tube in the embodiment of fig. 1 according to an embodiment of the present invention.

Fig. 4 is a schematic diagram illustrating the operation of step S10 in the embodiment of fig. 3.

Fig. 5 is a schematic diagram illustrating the operation of step S20 in the embodiment of fig. 3.

Fig. 6 is a schematic diagram illustrating the operation of step S30 in the embodiment of fig. 3.

Fig. 7 is a schematic diagram illustrating the operation of step S40 in the embodiment of fig. 3.

Fig. 8 is a schematic diagram illustrating the operation of step S50 in the embodiment of fig. 3.

Fig. 9 is a schematic diagram illustrating the operation of step S51 in the embodiment of fig. 3.

Fig. 10 is a schematic diagram illustrating the operation of step S52 in the embodiment of fig. 3.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative only and should not be construed as limiting the invention.

As used herein, the singular forms "a", "an", "the" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, or operations, but do not preclude the presence or addition of one or more other features, integers, steps, operations, or groups thereof.

It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Referring to fig. 1-2, a chamber is disposed in thecentrifuge tube 100, afirst piston 10, asecond piston 20 and aflexible connecting tube 30 are disposed in the chamber, the chamber is sequentially divided into afirst cavity 101, asecond cavity 102 and athird cavity 103 by thefirst piston 10 and thesecond piston 20, thesecond cavity 102 is formed between thefirst piston 10 and thesecond piston 20 at an interval, thefirst cavity 101 is formed between thefirst piston 10 and one end of the chamber at an interval, and thethird cavity 103 is formed between thesecond piston 20 and the other end of the chamber at an interval.

The piston is characterized in that a first connectingport 41 and a second connectingport 42 which are communicated with the outside are arranged in the cavity, a third connectingport 11 is arranged on thefirst piston 10, the first connectingport 41 is located in thefirst cavity 101, the second connectingport 42 is located in thethird cavity 103, one end of the flexible connectingpipe 30 is connected with the first connectingport 41, the other end of the flexible connecting pipe is connected with the third connectingport 11, the flexible connecting pipe is communicated with the outside and the second cavity, and the outside and the third cavity are communicated through the second connecting port.

Thecentrifuge tube 100 is provided with afirst plug 104 and asecond plug 105, thefirst plug 104 is used for plugging thefirst connection port 41, and thesecond plug 105 is used for plugging thesecond connection port 42.

According to the centrifugal tube, thefirst piston 10 and thesecond piston 20 are arranged to form thefirst cavity 101, thesecond cavity 102 and thethird cavity 103, and thesecond cavity 102 and thethird cavity 103 are respectively used as the first reaction cavity and the second reaction cavity, so that two centrifugal operations can be completed through the samecentrifugal tube 100, and the storage difficulty and the operation difficulty are reduced.

The cavity still is equipped with the fourth connector, just still be equipped with on the centrifuging tube withthird end cap 106 that the fourth connector corresponds, the fourth connector is used for communicateing outside and first cavity, so that first piston can remove along the cavity inner wall.

Specifically, thefirst plug 104 and/or thesecond plug 105 include anouter layer 1041 made of a hard material and aninner layer 1042 made of an elastic material, a strip-shaped sealing opening 1043 is formed in an outward side of theinner layer 1042, the strip-shaped sealing opening 1043 is sealed by an elastic force under the action of no external force, and the strip-shaped sealing opening 1043 is spread open under the action of the external force so that a needle of thesyringe 200 can pass through the strip-shaped sealing opening 1043. According to the invention, by arranging theouter layer 1041 incapable of elastic deformation and theinner layer 1042 capable of elastic deformation, theinner layer 1042 is sealed under the action of elastic force without external force, and the strip-shaped sealing port 1043 is expanded under the action of external force so as to enable a needle of theinjector 200 to penetrate through to communicate the cavity with theinjector 200.

Preferably, thefirst plug 104 and/or thesecond plug 105 further comprises asleeve 1044, and the inner diameter of thesleeve 1044 is adapted to the outer diameter of the needle of the penetratedsyringe 200.

In other embodiments, in order to achieve the sealing effect, thefirst plug 104 and/or thesecond plug 105 may be provided with a valve to achieve sealing and communication between thesecond cavity 102 and thethird cavity 103.

Preferably, abase 50 is arranged at one end of thecentrifugal tube 100 where thesecond plug 105 is installed, and thebase 50 is used for hiding thesecond plug 105 and providing a supporting function; according to the centrifugal tube, thebase 50 is arranged, so that thecentrifugal tube 100 can be directly placed on a plane, and thesecond plug 105 is hidden, so that thesecond plug 105 is prevented from being abraded in the storage process, and a certain attractive effect is achieved.

Specifically, thefirst connector port 41 is provided with a first elastic tubular body 411, and the first elastic tubular body 411 is used for contacting and propping the inner wall of the flexible connectingpipe 30 for fixing; thethird connection port 11 is provided with a second elastictubular body 111, and the second elastictubular body 111 is used for contacting and spreading the inner wall of theflexible connection pipe 30 to fix.

The present invention utilizes the elastic force of the elastic structure to fix theflexible connection tube 30, which is not only convenient for assembly, but also can realize interference fit to prevent blood from leaking into thefirst cavity 101.

Preferably, thesecond connection port 42 is provided with abottom tube 421, thesecond piston 20 is provided with agroove 21 corresponding to thebottom tube 421, the shape of thegroove 21 corresponds to the shape of thebottom tube 421, and thebottom tube 421 penetrates through the tube wall of thecentrifugal tube 100 and thesecond piston 20.

According to the invention, thebottom tube 421 is arranged to change the flow direction of the fluid in the pumpingthird cavity 103, so that on one hand, the situation that the pumping amount cannot be accurately controlled due to direct bottom pumping is avoided, on the other hand, thebottom tube 421 is arranged to enable the bottom tube to firstly pump the platelet-poor plasma layer (PPP) on the upper layer and then to invert to preferentially obtain the platelet-rich buffy coat (PRP) on the lower layer, and therefore, the purity of the obtained platelet-rich buffy coat (PRP) is improved.

Specifically, thecentrifuge tube 100 further comprises afirst sealing ring 107 and asecond sealing ring 108, thefirst sealing ring 107 is arranged between thefirst piston 10 and the inner wall of thecentrifuge tube 100, and thesecond sealing ring 108 is arranged between thesecond piston 20 and the inner wall of thecentrifuge tube 100; thefirst sealing ring 107 and thesecond sealing ring 108 are arranged to effectively prevent the fluid in thesecond cavity 102 and thethird cavity 103 from leaking out, so that mutual contamination of blood products is avoided.

Referring to fig. 3, the present invention further provides a PRP extraction method using thecentrifugal tube 100, which includes the following steps:

s10, injecting blood into thesecond cavity 102 through thefirst port 41.

As shown in fig. 4, by using thesyringe 200 to introduce the untreated blood into thesecond chamber 102 through thefirst connection port 41, theflexible connection tube 30 and thethird connection port 11, thefirst piston 10 is moved toward thefirst connection port 41 by the blood to use thesecond chamber 102 as a storage chamber at the time of the first centrifugation operation.

S20, thecentrifuge tube 100 is subjected to a first centrifugation to separate the blood product into an upper layer of platelets and plasma suspension and a lower layer including red blood cells.

As shown in fig. 5, after the first centrifugation of the blood in thesecond chamber 102, the blood is separated into an upper layer of platelet and plasma suspension and a lower layer containing red blood cells; wherein the upper layer of the platelet and plasma suspension is the starting material for the second centrifugation operation.

S30, the mixture of platelets and plasma suspension is withdrawn through thefirst port 41.

As shown in fig. 6, the first purification process is completed by drawing out the mixture of platelets and plasma suspension using asyringe 200.

S40, a mixture of platelets and plasma suspension is injected into thethird cavity 103 through thesecond connection port 42.

As shown in fig. 7, the mixture of the extracted platelets and the plasma suspension is injected into thethird chamber 103 through thesecond connection port 42 by thesyringe 200, and thesecond piston 20 is moved toward thefirst connection port 41 by the mixture to use thethird chamber 103 as a storage chamber for the second centrifugation

S50, thecentrifuge tube 100 is centrifuged a second time to separate the mixture in thethird chamber 103 into an upper platelet poor plasma layer and a lower platelet rich buffy coat layer.

As shown in fig. 8, after the second centrifugation of the mixture in thethird chamber 103, the mixture is separated into an upper platelet poor plasma layer (PPP) and a lower platelet rich buffy coat (PRP), which is the material to be obtained.

And S60, drawing the platelet rich buffy coat through thesecond port 42.

The present invention sucks the platelet-rich buffy coat (PRP) at the bottom through thesecond connection port 42 to accomplish the purpose of extracting PRP.

In an embodiment of the present invention, thesecond connection port 42 is provided with abottom tube 421, thesecond piston 20 is provided with agroove 21 corresponding to thebottom tube 421, the shape of thegroove 21 corresponds to the shape of thebottom tube 421, and thebottom tube 421 is inserted into the tube wall of thecentrifuge tube 100 and thesecond piston 20.

After step S50, step S60 is preceded by:

and S51, drawing the platelet poor plasma layer through the second connectingport 42.

S52, inverting thecentrifuge tube 100.

As shown in fig. 9-10, the present invention first sets thebottom tube 421 so that it can suck the platelet poor plasma layer (PPP) located at the upper layer, and then turns thecentrifuge tube 100 upside down to perform step S60 to preferentially obtain the platelet rich buffy coat (PRP) located at the lower layer.

The invention utilizes the design of thebottom tube 421 and thegroove 21, so that when the amount of the fluid in thethird cavity 103 is reduced to a certain degree, the fluid pumped by theinjector 200 comes from thegroove 21, and theelongated groove 21 is utilized to more accurately control and suck the platelet rich buffy coat (PRP), and avoid sucking the redundant platelet poor plasma layer (PPP), thereby improving the purity of the obtained platelet rich buffy coat (PRP).

Throughout the description and claims of this application, the words "comprise" and variations of the words "comprises" and "comprising" are used to specify the presence of the stated features, values, steps or components.

Some features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, certain features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable combination in different embodiments.

The embodiments of the centrifuge tube and the PRP extraction method using the centrifuge tube according to the present invention are described in detail above. Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (10)

1. A centrifugal tube is characterized in that a cavity is arranged in the centrifugal tube, a first piston, a second piston and a flexible connecting pipe are arranged in the cavity, the cavity is sequentially divided into a first cavity, a second cavity and a third cavity by the first piston and the second piston, the cavity is provided with a first connecting port and a second connecting port which are communicated with the outside, the first piston is provided with a third connecting port, the flexible connecting pipe is located in the first cavity, one end of the flexible connecting pipe is connected with the first connecting port, the other end of the flexible connecting pipe is connected with the third connecting port, the flexible connecting pipe is used for communicating the second cavity with the outside, and the second connecting port is used for communicating the third cavity with the outside;

the centrifugal tube is provided with a first plug and a second plug, the first plug is used for plugging the first connecting port, and the second plug is used for plugging the second connecting port.

2. The centrifuge tube of claim 1, wherein the first connection port is provided with a first elastic tubular body for contacting and spreading the inner wall of the flexible connection tube for fixation.

3. The centrifuge tube of claim 1, wherein the third connection port is provided with a second elastic tubular body for contacting and spreading the inner wall of the flexible connection tube for fixation.

4. The centrifuge tube of claim 1, wherein the second connection port is provided with a bottom tube, the second piston is provided with a groove corresponding to the bottom tube, the groove has a shape corresponding to the bottom tube, and the bottom tube penetrates through the tube wall of the centrifuge tube and the second piston.

5. The centrifuge tube of claim 1, further comprising a first seal ring and a second seal ring, wherein the first seal ring is disposed between the first piston and the inner wall of the centrifuge tube, and the second seal ring is disposed between the second piston and the inner wall of the centrifuge tube.

6. The centrifuge tube of claim 1, wherein the first plug comprises an outer layer made of a hard material and an inner layer made of an elastic material, the inner layer is provided with a strip-shaped sealing opening on one side facing outwards, the strip-shaped sealing opening is sealed by an elastic force without an external force, and the strip-shaped sealing opening is spread open under the external force so as to allow a syringe needle to pass through.

7. The centrifuge tube of claim 1, wherein the second plug comprises an outer layer made of a hard material and an inner layer made of an elastic material, the inner layer is provided with a strip-shaped sealing opening on the outward side, the strip-shaped sealing opening is sealed by an elastic force without an external force, and the strip-shaped sealing opening is spread open under the external force to allow the syringe needle to pass through.

8. The centrifuge tube of claim 1, wherein a base is arranged at one end of the centrifuge tube where the second plug is installed, and the base is used for hiding the second plug and providing a supporting function.

9. A PRP extraction method using the centrifuge tube according to any of claims 1 to 8, characterized in that the method comprises the following steps:

injecting blood into the second cavity through the first port;

subjecting the centrifuge tube to a first centrifugation to separate an upper layer of platelet and plasma suspension and a lower layer of blood product comprising red blood cells;

extracting a mixture of platelets and plasma suspension through the first port;

injecting a mixture of platelets and plasma suspension into the third chamber through the second port;

centrifuging the centrifuge tube a second time to separate the mixed solution in the third chamber into an upper layer of a platelet poor plasma layer and a lower layer of a platelet rich buffy coat;

the platelet rich buffy coat is withdrawn through the second port.

10. The PRP extraction method according to claim 9, wherein the second connection port is provided with a bottom tube, the second piston is provided with a groove corresponding to the bottom tube, the shape of the groove corresponds to the shape of the bottom tube, and the bottom tube is inserted through the tube wall of the centrifuge tube and the second piston;

after "centrifuging the centrifuge tube for the second time to separate the mixed solution in the third chamber into the upper layer of the platelet poor plasma layer and the lower layer of the platelet rich buffy coat", before "extracting the platelet rich buffy coat through the second connection port by a syringe" further includes:

drawing the platelet poor plasma layer through the second connector;

the centrifuge tube was inverted.

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