CN112747989B - Automatic dyeing box for tissue slice - Google Patents

Abstract

The application discloses automatic dyeing case of tissue slice, including box and dyeing mechanism, the integration is provided with the dropping liquid subassembly on the base member of dyeing mechanism, even liquid subassembly and raffinate recovery subassembly, and the dropping liquid subassembly is used for dropping the dyeing liquid on the slide glass, and even liquid subassembly is used for spreading evenly the dyeing liquid that drops on the slide glass, and the raffinate is retrieved the subassembly and is used for absorbing the unnecessary dyeing liquid of dyeing back and retrieves the discharge to carry out repeated dyeing many times to the tissue slice on the slide glass. The multiple components are matched with each other, the repeated dyeing process of tissue section dropping liquid, liquid spreading and liquid absorbing is realized, a better dyeing effect is obtained, the integral structure of the device is reasonable, the integral arrangement of the device enables the dyeing mechanism to tend to be miniaturized, the automation control degree is higher, and the operation is simple and convenient.

Description

Automatic staining box for tissue slices

Technical Field

The application relates to the technical field of pathological tissue equipment, in particular to an automatic staining box for tissue slices.

Background

Hematoxylin-eosin staining (HE staining) is one of the most widely used routine staining of pathological tissue sections, and 70% -80% of the diagnoses in pathology are currently derived from this method. From the world, a considerable part of the pathology departments adopt a manual method or a semi-automatic method at present, the method has a plurality of steps, does not comprise previous sections and the like, more than 20 steps are carried out from the baking, dyeing, dehydrating and sealing of the light, and each step has the possibility of error, thereby bringing unknown risks to diagnosis. The staining is used as a core step of tissue slice treatment, and plays a decisive role in subsequent observation and diagnosis of the tissue slices. Dyeing liquid of multiple difference need be used to the dyeing process, and current dyeing apparatus degree of automation control degree is not high, and is inefficient, and the function is more single, only can realize the process that the dropping liquid was rendered up, and dyeing process effect can't be guaranteed.

Therefore, the automatic tissue section mounting equipment which has high automation degree, can realize automatic and accurate control, avoids manual operation and tissue section cross contamination, is environment-friendly, convenient, efficient and good in consistency is very necessary.

Disclosure of Invention

The present application provides an automatic staining box for tissue slices to solve at least one of the above technical problems.

The technical scheme adopted by the application is as follows:

the utility model provides an automatic dyeing case of tissue slice, includes the box and dyeing mechanism in the box, dyeing mechanism includes the base member, the base member is provided with:

the liquid dropping assembly comprises a liquid inlet, a liquid dropping pipeline, a first control valve and a liquid discharging port, the liquid discharging port is arranged at the bottom of the base body, the liquid dropping pipeline is communicated with the liquid inlet and the liquid discharging port, and the first control valve is arranged on the liquid dropping pipeline and controls the liquid dropping pipeline to be switched on and off;

the liquid homogenizing assembly comprises a first air inlet, a gas transmission pipeline, a second control valve and a first exhaust port, the first exhaust port is arranged adjacent to the liquid outlet, the gas transmission pipeline is communicated with the first air inlet and the first exhaust port, and the second control valve is arranged on the gas transmission pipeline and controls the on-off of the gas transmission pipeline;

and the residual liquid recovery assembly comprises a liquid suction port and a recovery pipeline, wherein the liquid suction port is arranged at the bottom of the base body, and the recovery pipeline is connected with the liquid suction port and forms negative pressure at the liquid suction port.

Further, the dropping liquid subassembly is still including mixing the liquid chamber, the leakage fluid dram includes a plurality of son leakage fluid dram, the dropping liquid pipeline via mix the liquid chamber will the inlet with son leakage fluid dram is linked together.

Further, the second control valve is communicated with the liquid mixing cavity and controls air flow to be discharged through the first exhaust port or the liquid mixing cavity.

Furthermore, the residual liquid recovery assembly further comprises a second air inlet, an exhaust pipeline, a third control valve and a second exhaust port, the second exhaust port is adjacent to the liquid suction port, the exhaust pipeline is communicated with the second air inlet and the second exhaust port, and the third control valve is arranged on the exhaust pipeline and controls the on-off of the exhaust pipeline.

Further, the liquid homogenizing assembly further comprises a first gas distribution cavity, the first exhaust port comprises a plurality of first sub exhaust ports, and the gas transmission pipeline enables the first gas inlet and the first sub exhaust ports to be communicated through the first gas distribution cavity.

Further, the residual liquid recovery assembly further comprises a second gas distribution chamber, the second gas outlet comprises a plurality of second sub gas outlets, and the gas outlet pipeline communicates the second gas inlet with the second sub gas outlets through the second gas distribution chamber.

Further, the third control valve communicates with the first branch air chamber and controls the discharge of the air flow through the first exhaust port or the second exhaust port.

Furthermore, the plurality of second sub-exhaust ports are uniformly arranged in a V shape and enclose the liquid suction port, and the arrangement included angle alpha of the plurality of second sub-exhaust ports is 80-100 degrees.

Further, the plurality of second sub-exhaust ports protrude out of the substrate and are obliquely arranged toward the liquid suction port.

Further, the dyeing machine also comprises a fixing frame, and four dyeing components are symmetrically and uniformly arranged on the fixing frame.

Due to the adoption of the technical scheme, the beneficial effects obtained by the application are as follows: the dyeing mechanism of the automatic tissue section dyeing box can freely control and move in the box body so as to dye tissues on a glass slide. The integrated dropping liquid subassembly, even liquid subassembly and the raffinate recovery subassembly that is provided with on dyeing mechanism's the base member, the dropping liquid subassembly is used for dropping on the slide with the dyeing liquid, and even liquid subassembly is used for evenly spreading out the dyeing liquid that drops on the slide, and the raffinate is retrieved the subassembly and is used for absorbing the unnecessary dyeing liquid of dyeing back and retrieving the discharge to carry out repeated dyeing many times to the tissue section on the slide. The multiple components are matched with each other, the repeated dyeing process of tissue section dropping liquid, liquid spreading and liquid absorbing is realized, a better dyeing effect is obtained, the integral structure of the device is reasonable, the integral arrangement of the device enables the dyeing mechanism to tend to be miniaturized, the automation control degree is higher, and the operation is simple and convenient.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a schematic structural diagram of a part of the components of an automatic tissue slice staining box provided by the present application.

Fig. 2 is a schematic structural view of the dyeing mechanism in fig. 1.

Fig. 3 is a schematic view of another angle of the dyeing mechanism in fig. 1.

Fig. 4 is a schematic front view of the dyeing mechanism in fig. 1.

Fig. 5 is a schematic top view of the dyeing mechanism in fig. 1.

Fig. 6 is a schematic bottom view of the dyeing mechanism in fig. 1.

Fig. 7 is a left side view schematic diagram of the dyeing mechanism in fig. 1.

FIG. 8 is a schematic sectional view of the dyeing means of FIG. 3 taken along the plane of theloading port 4 b.

FIG. 9 is a schematic sectional view of the dyeing apparatus of FIG. 3 taken along the plane of theloading port 4 a.

Fig. 10 is a schematic cross-sectional view of the dyeing mechanism of fig. 3 taken along the plane of the second control valve.

FIG. 11 is a schematic cross-sectional view of the dyeing mechanism of FIG. 3 taken along a plane in which the third control valve is located.

Fig. 12 is a schematic sectional view of the dyeing mechanism in fig. 6 along the plane of the second air separation chamber.

Wherein,

1 fixed frame, 2 dyeing mechanisms, 3 substrates, 4/4a/4b liquid inlets, 5 liquid dropping pipelines, 6 first control valves, 7 liquid outlets, 8 first air inlets, 9 gas transmission pipelines, 10 second control valves, 11 first air outlets, 12 liquid suction ports, 13 recovery pipelines, 14 liquid mixing cavities, 15 sub liquid outlets, 16 first gas distribution cavities, 17 first sub air outlets, 18 second air inlets, 19 air exhaust pipelines, 20 third control valves, 21 second air outlets, 22 second gas distribution cavities and 23 second sub air outlets.

Detailed Description

In order to more clearly explain the overall concept of the present application, the following detailed description is given by way of example in conjunction with the accompanying drawings.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, however, the present application may be practiced in other ways than those described herein, and therefore the scope of the present application is not limited by the specific embodiments disclosed below.

In addition, in the description of the present application, it is to be understood that the terms "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," and the like are used in the positional or orientational relationship shown in the drawings for the purpose of convenience and simplicity of description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the present invention.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and encompass, for example, both fixed and removable connections or integral parts thereof; the connection can be mechanical connection, electrical connection or communication; they may be directly connected or indirectly connected through intervening media, or may be connected through the use of two elements or the interaction of two elements. The specific meanings of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. In the description herein, reference to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

As shown in fig. 1 to 12, the present application provides an automatic staining box of tissue slice, including the box and staining mechanism 2 in the box, staining mechanism 2 includes base member 3, base member 3 is provided with:

the liquid dropping assembly comprises aliquid inlet 4, aliquid dropping pipeline 5, afirst control valve 6 and aliquid discharging port 7, wherein theliquid discharging port 7 is arranged at the bottom of the base body 3, theliquid dropping pipeline 5 is communicated with theliquid inlet 4 and theliquid discharging port 7, and thefirst control valve 6 is arranged on theliquid dropping pipeline 5 and controls the on-off of theliquid dropping pipeline 5;

the liquid homogenizing assembly comprises afirst air inlet 8, an air conveying pipeline 9, asecond control valve 10 and afirst exhaust port 11, thefirst exhaust port 11 is arranged adjacent to theliquid discharging port 7, the air conveying pipeline 9 is communicated with thefirst air inlet 8 and thefirst exhaust port 11, and thesecond control valve 10 is arranged on the air conveying pipeline 9 and controls the on-off of the air conveying pipeline 9;

the residual liquid recovery assembly comprises aliquid suction port 12 and arecovery pipeline 13, wherein theliquid suction port 12 is arranged at the bottom of the base body 3, therecovery pipeline 13 is connected with theliquid suction port 12, and negative pressure is formed at theliquid suction port 12.

The staining mechanism 2 of the automatic staining case of this application tissue slice can freely control in the box and remove to stain the tissue on the slide glass. The integrated dropping liquid subassembly, even liquid subassembly and the raffinate recovery subassembly that is provided with on dyeing mechanism 2's the base member 3, the dropping liquid subassembly is used for dropping on the slide with the dyeing liquid, and even liquid subassembly is used for evenly spreading the dyeing liquid that drops on the slide, and the raffinate is retrieved the subassembly and is used for absorbing the unnecessary dyeing liquid of back with dyeing and retrieve the discharge to carry out repeated dyeing many times to the tissue section on the slide. The multiple components are matched with each other, so that the repeated dyeing process of tissue section dropping, liquid spreading and liquid absorbing is realized, a better dyeing effect is obtained, the integral structure of the device is reasonable, the integral arrangement of the dyeing mechanism 2 tends to miniaturization, the automation control degree is higher, and the operation is simple and convenient.

In the structure, thefirst control valve 6 of the liquid dropping component can realize the on-off control of theliquid dropping pipeline 5, and the automatic control performance of the liquid dropping process of the tissue section is improved. During liquid dropping, thefirst control valve 6 controls theliquid inlet 4 to be communicated with theliquid outlet 7, so that the staining solution is convenient to drop on the glass slide from theliquid outlet 7 at the bottom of the substrate 3 to stain the tissue section; when the dripping is finished, thefirst control valve 6 controls to disconnect theliquid inlet 4 from theliquid outlet 7, and then the dripping is stopped.

Thesecond control valve 10 of the liquid homogenizing assembly can realize on-off control of the gas transmission pipeline 9, and automatic controllability of the dropping liquid homogenizing process is improved, so that the dyeing liquid is uniformly dispersed on the glass slide. After the liquid dropping is finished, thesecond control valve 10 controls thefirst air inlet 8 to be communicated with thefirst air outlet 11, so that the air flow is discharged through thefirst air outlet 11 to uniformly blow and spread the liquid dropping; after the dropping liquid is uniformly distributed, thesecond control valve 10 controls to disconnect thefirst air inlet 8 and thefirst air outlet 11, and the blowing is stopped.

The residual liquid recovery assembly is arranged at aliquid suction port 12 at the bottom of the base body 3 to form negative pressure, and after the even dropping liquid finishes the dyeing effect, theliquid suction port 12 sucks and collects redundant dyeing liquid on the glass slide to timely clean the glass slide, so that the tissue section on the glass slide is repeatedly dyed for multiple times, the dyeing efficiency is improved, and the dyeing effect is ensured.

Specifically, as shown in fig. 8 and 9, the dropping assembly is provided with 8liquid inlets 4,4 as one group, which are divided into two groups, and 8dropping pipelines 5 and 8first control valves 6,8 can correspond to 8 different coloring agents one by one, respectively. The liquid homogenizing assembly is provided with 1first air inlet 8, corresponds to the air transmission pipeline 9 and thesecond control valve 10, and thefirst air inlet 8 is communicated with an external air source. The residual liquid recovery assembly is provided with 1liquid suction port 12 corresponding to the correspondingrecovery pipeline 13, therecovery pipeline 13 is connected with a vacuum pump or other vacuum air sources, theliquid suction ports 12 generate negative pressure, the gathered reagent is sucked and recovered, and the reagent is discharged to a waste liquid tank.

Further, the dropping liquid assembly further comprises a liquid mixingcavity 14, theliquid discharge port 7 comprises a plurality of subliquid discharge ports 15, and theliquid inlet 4 is communicated with the subliquid discharge ports 15 through the liquid mixingcavity 14 by the droppingliquid pipeline 5.

Mixliquid chamber 14 and can fully mix different staining fluids, avoid single staining fluid to act on tissue section and influence the dyeing effect, and then bring inconvenience for follow-up operation process. Theleakage fluid dram 7 sets up a plurality of sub-leakagefluid dram 15, and a plurality of sub-leakagefluid dram 15 can effectively improve flowing back dropping liquid efficiency on the one hand, and on the other hand can evenly flow back the dropping liquid, avoids the staining fluid too concentrated at the tissue slice, guarantees the dyeing effect.

During the dropping liquid, different dyeing liquid gets into droppingliquid pipeline 5 byinlet 4 that corresponds separately respectively, and the dyeing liquid flows throughfirst control valve 6 and gets into to mix in the liquid chamber 14 (each droppingliquid pipeline 5 all is linked together with mixing liquid chamber 14), and different dyeing liquid mixes the back in mixingliquid chamber 14, via a plurality of sonleakage fluid dram 15 discharge drippage on the slide glass (each sonleakage fluid dram 15 all is linked together with mixing liquid chamber 14).

Specifically, as shown in fig. 2 and 6, in the present invention, 7 subliquid discharge ports 15,7 and 15 sub liquid discharge ports are provided in a linear shape and arranged uniformly on the bottom of the base 3. Theliquid mixing cavity 14 is a tube cavity structure which is arranged inside the machine body and along the arrangement direction of a plurality of subliquid discharge ports 15, and is respectively communicated with 8 differentliquid dropping pipelines 5 and 7 subliquid discharge ports 15. The secondaryliquid outlet 15 and the liquid inlet 4 (the liquid dropping pipeline 5) are arranged independently, and the respective quantity can be freely set according to the requirement.

Further, thesecond control valve 10 communicates with theliquid mixing chamber 14, and controls the air flow to be discharged through thefirst exhaust port 11 or theliquid mixing chamber 14.

As shown in fig. 10, thesecond control valve 10 disposed on the gas transmission pipeline 9 controls the on-off of the gas transmission pipeline 9 and communicates with the liquid mixingcavity 14, and when thesecond control valve 10 controls the communication of the gas transmission pipeline 9, it communicates thefirst gas inlet 8 with thefirst gas outlet 11, so as to uniformly blow and spread the dropping liquid; when thesecond control valve 10 is controlled to disconnect the gas pipeline 9, thefirst gas inlet 8 is communicated with the liquid mixingcavity 14, so that the liquid mixingcavity 14 and theliquid discharge port 7 are cleaned, the accumulation of the dyeing liquid is avoided, and the interference to the subsequent dyeing process is prevented.

Specifically, after the dyeing is completed for one time, the residual reagent in theliquid mixing chamber 14 and theliquid discharge port 7 needs to be blown out by using compressed air by using the cleaning function of the dyeing mechanism 2, so as to ensure the stability and quality of the reagent in the next dyeing. The cleaning function is realized mainly by introducing compressed air into thefirst air inlet 8, and after the compressed air enters the air transmission pipeline 9 of the base body 3, the gas exhausted from thefirst air outlet 11 is turned to theliquid outlet 7 to be exhausted by utilizing the reversing of thesecond control valve 10, and under the action of air pressure, the residual reagent in the liquid mixingcavity 14 and theliquid outlet 7 is blown out.

Further, as shown in fig. 11, the residual liquid recovering assembly further includes asecond gas inlet 18, an exhaust pipeline 19, athird control valve 20 and a second exhaust port 21, the second exhaust port 21 is disposed adjacent to theliquid suction port 12, the exhaust pipeline 19 communicates thesecond gas inlet 18 and the second exhaust port 21, and thethird control valve 20 is disposed in the exhaust pipeline 19 and controls on/off of the exhaust pipeline 19.

In order to improve the absorption and collection effects of the residual liquid recovery assembly on the excess staining solution of the glass slide, the residual liquid recovery assembly is further provided with asecond air inlet 18 and a second air outlet 21, and the second air outlet 21 is arranged adjacent to theliquid suction port 12 so as to gather and collect the excess staining solution on the glass slide and facilitate the absorption and collection work of theliquid suction port 12. When the device is used, airflow enters the exhaust pipeline 19 through thesecond air inlet 18, thethird control valve 20 controls and communicates with the exhaust pipeline 19, the airflow is discharged from the second air outlet 21 to blow and move redundant staining solution on a glass slide, the excess staining solution is collected and gathered, then thesolution suction port 12 aligns the gathered staining solution to perform negative pressure suction and collection, and the collected staining solution is discharged into the waste liquid tank.

Further, the liquid homogenizing assembly further comprises a firstgas dividing chamber 16, thefirst exhaust port 11 comprises a plurality of firstsub exhaust ports 17, and the gas pipeline 9 communicates thefirst gas inlet 8 with the firstsub exhaust ports 17 through the firstgas dividing chamber 16.

As shown in fig. 10, the firstair distribution chamber 16 communicates with the air delivery duct 9 and uniformly distributes the air flow to be discharged from the plurality of firstsub-air outlets 17 to uniformly blow the staining solution dropped on the slide glass. A plurality of firstsub-gas vents 17 can make the air current evenly give vent to anger on a large scale to improve the area of action to the dropping liquid, improve the homogeneity of blowing and spreading.

Specifically, as shown in fig. 6 and 10, in the present application, 7 firstsub-exhaust ports 17,7 firstsub-exhaust ports 17 are uniformly arranged in a straight line shape at the bottom of the base body 3, and correspond to the plurality ofsub-liquid exhaust ports 15, and the firstgas distribution chamber 16 is disposed inside the base body 3, along the pipe cavity structure of the arrangement direction of the plurality of sub-exhaust ports, and is respectively communicated with the gas transmission pipeline 9 and the plurality of firstsub-exhaust ports 17. The plurality of firstsub-exhaust ports 17 are also independently provided, and the number thereof can be freely set according to the requirement.

Further, the residual liquid recovery assembly further comprises a second sub-air-vent chamber 22, the second air-vent 21 comprises a plurality of second sub-air-vents 23, and the air-vent pipe 19 communicates the second air-vent 18 with the second sub-air-vents 23 through the second sub-air-vent chamber 22.

As shown in fig. 6 and 11, the second sub-air-distribution chamber 22 is communicated with the exhaust duct 19, and uniformly distributes the air flow and discharges the air flow through a plurality of secondsub-exhaust ports 23, so as to collect and gather the excess dyeing liquid. The plurality of secondsub-exhaust ports 23 can enable the airflow to uniformly emit air within a range, so that the acting area of the excessive dyeing liquid is increased, and the gathering effect of the excessive dyeing liquid is improved.

Further, thethird control valve 20 communicates with the firstbranch air chamber 16 and controls the air flow to be discharged through thefirst exhaust port 11 or the second exhaust port 21.

As shown in fig. 11, thethird control valve 20 disposed on the exhaust duct 19 controls the on/off of the exhaust duct 19 and communicates with the firstbranch air chamber 16, and when thethird control valve 20 controls the communication of the exhaust duct 19, it communicates thesecond air inlet 18 with the second air outlet 21 to collect and gather the excess dyeing liquid; when thethird control valve 20 controls to disconnect the exhaust duct 19, it may communicate thesecond air inlet 18 with the first air-dividingchamber 16 to perform a blowing action on the dropping liquid. The blowing direction of the air flow is controlled bidirectionally by thethird control valve 20, so that the usability and the automatic controllability of the equipment are effectively improved.

Further, in order to improve the gathering effect of the redundant dyeing liquid, the plurality of secondsub-exhaust ports 23 are uniformly arranged in a V shape and enclose theliquid suction port 12, and the arrangement included angle α of the plurality of secondsub-exhaust ports 23 is 80 ° to 100 °. The plurality of secondsub-exhaust ports 23 protrude from the substrate 3 and are disposed to be inclined toward theliquid suction port 12.

As shown in fig. 6 and 12, the bottom of the base 3 is provided with 18 secondsub-exhaust ports 23,9 protruding and inclined as one group, which are divided into two groups, wherein the two groups are arranged in a V-shape and enclosed at theliquid suction port 12, and the V-shaped included angle of the plurality of secondsub-exhaust ports 23 is 80 ° to 100 ° (90 ° in the figure). When the device is used, theliquid suction port 12 is operated and controlled to be aligned with the staining solution on the glass slide and move from one end to the other end, and meanwhile, the plurality of secondsub-exhaust ports 23 are used for collecting and gathering the staining solution, so that the suction efficiency and effect of theliquid suction port 12 on the redundant staining solution are improved, and the usability and the controllability of the device are improved. The second sub-exhausts are also arranged independently, and the number of the second sub-exhausts can be freely set according to the requirement.

Further, as shown in fig. 1, the automatic tissue section staining box of the present application further includes a fixing frame 1, and four staining mechanisms 2 are symmetrically and uniformly arranged on the fixing frame 1. As shown in the figure, four dyeing mechanism 2 even symmetric arrangement are on mount 1, and system control drive mount 1 removes in order to drive four dyeing mechanism 2 to control four dyeing mechanism 2 and carry out the liquid dropping-spread even-washing-imbibition to a plurality of tissue slices simultaneously and repeat the dyeing process many times, not only can obtain better dyeing effect, improve tissue slice's dyeing efficiency moreover greatly, easy operation is convenient, and degree of automation is high, satisfies medical personnel's user demand.

Where not mentioned in this application, this may be achieved using or taking advantage of existing technology.

The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims (8)

1. The utility model provides an automatic case that dyes of tissue slice, includes the box and dyeing mechanism in the box, its characterized in that, dyeing mechanism includes the base member, the base member is provided with:

the liquid dropping assembly comprises a liquid inlet, a liquid dropping pipeline, a first control valve and a liquid discharging port, the liquid discharging port is arranged at the bottom of the base body, the liquid dropping pipeline is communicated with the liquid inlet and the liquid discharging port, and the first control valve is arranged on the liquid dropping pipeline and controls the liquid dropping pipeline to be switched on and off;

the liquid homogenizing assembly comprises a first air inlet, a gas transmission pipeline, a second control valve and a first exhaust port, the first exhaust port is arranged adjacent to the liquid outlet, the gas transmission pipeline is communicated with the first air inlet and the first exhaust port, and the second control valve is arranged on the gas transmission pipeline and controls the on-off of the gas transmission pipeline;

the residual liquid recovery assembly comprises a liquid suction port and a recovery pipeline, the liquid suction port is arranged at the bottom of the base body, and the recovery pipeline is connected with the liquid suction port and forms negative pressure at the liquid suction port;

the residual liquid recovery assembly further comprises a second air inlet, an exhaust pipeline, a third control valve and a second exhaust port, the second exhaust port is adjacent to the liquid suction port, the exhaust pipeline is communicated with the second air inlet and the second exhaust port, and the third control valve is arranged on the exhaust pipeline and controls the on-off of the exhaust pipeline;

the residual liquid recovery assembly further comprises a second gas separation cavity, the second gas outlet comprises a plurality of second sub gas outlets, and the gas exhaust pipeline enables the second gas inlet and the second sub gas outlets to be communicated through the second gas separation cavity; the plurality of second sub-exhaust ports are uniformly distributed in a V shape and enclose the liquid suction port.

2. The automatic tissue slice staining cassette of claim 1, wherein the drip assembly further comprises a fluid mixing chamber, the fluid outlet comprises a plurality of sub fluid outlets, and the drip conduit communicates the fluid inlet with the sub fluid outlets via the fluid mixing chamber.

3. The automatic tissue slice staining box of claim 2, wherein the second control valve is in communication with the liquid mixing chamber and controls the air flow to be discharged through the first exhaust port or the liquid mixing chamber.

4. The automatic staining box for tissue slices of claim 1, wherein the homogenizing assembly further comprises a first air dividing chamber, the first air outlet comprises a plurality of first sub air outlets, and the air conveying pipeline communicates the first air inlet with the first sub air outlets through the first air dividing chamber.

5. The automatic tissue slice staining cassette of claim 4, wherein the third control valve communicates with the first gas distribution chamber and controls the flow of gas to be discharged through the first exhaust port or the second exhaust port.

6. The automatic tissue slice staining cassette of claim 1, wherein the second plurality of sub-vents are arranged at an angle α of 80 ° to 100 °.

7. The automatic staining kit for tissue slices of claim 6, wherein the second plurality of sub-exhaust ports protrude from the base body and are disposed obliquely toward the liquid suction port.

8. The automatic tissue slice staining cassette of claim 1, further comprising a holder, wherein four staining assemblies are symmetrically and uniformly arranged on the holder.

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