CN112730364A - Detector for detecting heparin content in blood - Google Patents

Abstract

The invention discloses a detector for detecting the heparin content in blood, which comprises a shell, a microfluidic chip arranged in the shell, a laser-induced fluorescence detection device for detecting the heparin concentration in the blood, a Bluetooth module for sending heparin concentration data to an upper computer, and a controller for receiving the heparin concentration detected by the laser-induced fluorescence detection device and sending the data to the upper computer through the Bluetooth module; the shell is provided with a micro-channel communicated with an inlet of the micro-fluidic chip, and the micro-channel is communicated with the intravenous injection needle. The micro-fluidic chip filters blood, the blood is mixed with a reagent for reaction, fluorescent substances in the mixture are enriched, and the laser-induced fluorescence detection device detects the concentration of heparin in the blood at the outlet end of the micro-fluidic chip. The invention can realize real-time monitoring of the heparin content and the change condition of the heparin content. When the content reaches the dangerous index, a warning is given to ensure the safety of the patient.

Description

Detector for detecting heparin content in blood

Technical Field

The invention relates to a detector, in particular to a detector for detecting the heparin content of blood on line.

Background

Heparin is a highly sulfated mucopolysaccharide that binds to antithrombin and thereby greatly enhances the inhibitory effects of antithrombin on thrombin and other coagulation factors. Heparin is widely used clinically as a preventive and therapeutic agent for related diseases, particularly as an anticoagulant in surgery. However, excessive use of heparin causes side effects such as massive bleeding, thrombocytopenia, hyperkalemia, osteoporosis, and the like, and thus the dosage of heparin must be strictly monitored. The current clinical methods for heparin quantitative determination include activated clotting time method (ACT) and activated partial thromboplastin time method (APTT). However, the two heparin detection methods have the defects of long time consumption, high price, indirect detection, unreliable data and the like; 2. colorimetric, absorption and electrochemical immunoassay methods; although these methods have good sensitivity and specificity, they are complicated, time-consuming, inconvenient to use, and unable to perform real-time detection, resulting in low accuracy.

Disclosure of Invention

In view of the above problems, an object of the present invention is to provide a detector for detecting heparin content in blood. The invention can realize real-time monitoring of the heparin content and the change condition of the heparin content. When the content reaches the dangerous index, a warning is given to ensure the safety of the patient.

The purpose of the invention can be achieved by adopting the following technical scheme:

a detector for detecting the heparin content in blood comprises a shell, a microfluidic chip arranged in the shell, a laser-induced fluorescence detection device for detecting the heparin concentration in blood, a Bluetooth module for sending heparin concentration data to an upper computer, and a controller for receiving the heparin concentration detected by the laser-induced fluorescence detection device and sending the data to the upper computer through the Bluetooth module; the shell is provided with a micro-channel communicated with an inlet of the micro-fluidic chip, and the micro-channel is communicated with the intravenous injection needle. The micro-fluidic chip filters blood, the blood is mixed with a reagent for reaction, fluorescent substances in the mixture are enriched, and the laser-induced fluorescence detection device detects the concentration of heparin in the blood at the outlet end of the micro-fluidic chip.

As a preferred scheme, the microfluidic chip comprises a one-way valve for preventing blood from flowing backwards, a piezoelectric peristaltic micropump for controlling the flow rate of blood, a filtering module for filtering blood cells in the blood, a mixing module for combining a contained reagent with heparin in plasma to form a fluorescent substance, and an enrichment module for increasing the concentration of the fluorescent substance; the inlet of the one-way valve is communicated with the micro-channel, and the laser-induced fluorescence detection device detects the number of the fluorescent particles in the blood flowing out of the outlet of the enrichment module.

Preferably, an osmosis membrane is arranged in the enrichment module, and the blood removes water and inorganic salts under the action of the osmosis membrane, so that the concentration of the fluorescent substance is increased.

As a preferable scheme, a liquid storage tank is arranged in the housing, and the liquid storage tank is communicated with an outlet of the enrichment module.

Preferably, the upper computer is provided with a display for displaying the heparin concentration.

As a preferable scheme, a battery for supplying power to the laser-induced fluorescence detection device, the bluetooth module and the controller is arranged in the housing.

As a preferable scheme, the battery is a rechargeable battery, and a charging interface for charging the battery is arranged on the housing.

As a preferred scheme, the controller is a single chip microcomputer.

The implementation of the invention has the following beneficial effects:

1. when the micro-fluidic chip is used, the intravenous injection needle is connected with the micro-channel, and blood enters the micro-channel through the intravenous injection needle and enters the micro-fluidic chip through the micro-channel under the pushing of blood pressure. The microfluidic chip sequentially filters blood, mixes the blood with a reagent for reaction, enriches fluorescent substances in the mixture, and finally the laser-induced fluorescence detection device performs fluorescence detection on the blood flowing out of the microfluidic chip, so that the aim of detecting the concentration of heparin in the blood is fulfilled. The data obtained after detection are sent to the controller, the controller sends the data to the upper computer in a wireless transmission mode through the Bluetooth module and displays the data on the upper computer, so that the function of displaying the real-time concentration and the change condition of heparin is realized, and the detection accuracy is improved. When the concentration of heparin reaches the dangerous index, the upper computer sends out warning through loudspeaker and light, makes the doctor reduce the injection volume of heparin to guarantee patient's life safety. The whole detector has simple structure and convenient and quick operation and use.

2. Under the action of blood pressure, blood flows into the one-way valve and the piezoelectric peristaltic micropump in sequence through the microchannel. The one-way valve prevents the blood from flowing backwards, and the piezoelectric peristaltic micropump controls the flow rate of the blood. Blood flowing out of the piezoelectric peristaltic micropump flows through the filtering module; the filtering module filters blood cells in the blood, and then the filtered blood (hereinafter referred to as plasma) enters the mixing module; a reagent is attached to the mixing module, and the reagent is combined with heparin in plasma to form a fluorescent substance; then the plasma mixed with the reagent enters an enrichment module; the enrichment module is a pipeline with an upper half layer and a lower half layer, and a permeable membrane is arranged in the middle of the pipeline; plasma flows through first half layer pipeline and removes most of water and micromolecule material such as inorganic salt under the osmotic membrane effect, promotes fluorescent substance concentration in the plasma greatly, has promoted fluorescence detection efficiency and precision greatly.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of the structure of the detector for detecting heparin content in blood according to the present invention.

Fig. 2 is a schematic view of the internal structure of fig. 1.

Fig. 3 is a schematic structural diagram of a microfluidic chip of a detector for detecting heparin content in blood according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

Referring to fig. 1 and 2, the present embodiment relates to a detector for detecting heparin content in blood, which includes a housing 1, a microfluidic chip 2 disposed in the housing 1, a laser-induced fluorescence detection device 3 for detecting heparin concentration in blood, a bluetooth module 4 for sending heparin concentration data to an upper computer, and a controller for receiving the heparin concentration detected by the laser-induced fluorescence detection device 3 and sending the data to the upper computer through the bluetooth module 4; the shell 1 is provided with a micro-channel 11 communicated with the inlet of the micro-fluidic chip 2, and the micro-channel 11 is communicated with the intravenous injection needle. The micro-fluidic chip 2 filters blood, the blood is mixed with a reagent for reaction, fluorescent substances in the mixture are enriched, and the laser-induced fluorescence detection device 3 detects the concentration of heparin in the blood at the outlet end of the micro-fluidic chip 2. The controller is a single chip microcomputer.

When in use, the intravenous needle is connected with the micro-channel 11, and blood enters the micro-channel 11 through the intravenous needle under the pushing of blood pressure and enters the micro-fluidic chip 2 through the micro-channel 11. The micro-fluidic chip 2 filters blood, mixes the blood with a reagent for reaction, enriches fluorescent substances in the mixture, and finally the laser-induced fluorescence detection device 3 performs fluorescence detection on the blood flowing out of the micro-fluidic chip 2, thereby realizing the purpose of detecting the concentration of heparin in the blood. The data obtained after detection are sent to the controller, the controller sends the data to the upper computer in a wireless transmission mode through the Bluetooth module 4 and displays the data on the upper computer, so that the function of displaying the real-time concentration and the change condition of heparin is realized, and the detection accuracy is improved. When the concentration of heparin reaches the dangerous index, the upper computer sends out warning through loudspeaker and light, makes the doctor reduce the injection volume of heparin to guarantee patient's life safety. The whole detector has simple structure and convenient and quick operation and use.

As shown in fig. 3, the microfluidic chip 2 includes a one-way valve 21 for preventing blood from flowing backwards, a piezoelectricperistaltic micropump 22 for controlling the flow rate of blood, afiltering module 23 for filtering blood cells in blood, amixing module 24 for combining a contained reagent with heparin in plasma to form a fluorescent substance, and anenrichment module 25 for increasing the concentration of the fluorescent substance; the inlet of the one-way valve 21 is communicated with the micro-channel 11, and the laser-induced fluorescence detection device 3 detects the number of fluorescent particles in the blood flowing out from the outlet of theenrichment module 25. An osmotic membrane is arranged in theenrichment module 25, and water and inorganic salts are removed from blood under the action of the osmotic membrane, so that the concentration of the fluorescent substance is increased.

Under the action of blood pressure, the blood flows through the micro-channel 11 into the one-way valve 21 and the piezoelectricperistaltic micro-pump 22 in sequence. Thecheck valve 21 prevents the reverse flow of blood, and the piezoelectricperistaltic micro-pump 22 controls the flow rate of blood. The blood flowing from the piezoelectricperistaltic micropump 22 flows through thefiltration module 23; thefiltering module 23 filters blood cells in the blood, and then the filtered blood (hereinafter referred to as plasma) enters themixing module 24; a reagent is attached to themixing module 24, and the reagent is combined with heparin in plasma to form a fluorescent substance; then the plasma mixed with the reagent enters anenrichment module 25; theenrichment module 25 is a pipeline with an upper half layer and a lower half layer, and a permeable membrane is arranged in the middle of the pipeline; plasma flows through the upper layer pipeline, and most of small molecular substances such as water, inorganic salt and the like are removed under the action of the permeable membrane, so that the concentration of fluorescent substances in the plasma is greatly improved, and the fluorescence detection efficiency and precision are greatly improved; and finally, the plasma containing high-concentration fluorescent substances enters a detection area, the number of the fluorescent particles at the moment is detected under the detection of a laser-induced fluorescence detection device 3 (shown in figure 2, the device is arranged below the microfluidic chip 2) in figure 2, the data is sent to a controller, and the controller sends the data to an upper computer through a Bluetooth module 4. The plasma after flowing through the detection area finally enters the liquid storage tank, and the whole working process of the microfluidic chip 2 is completed.

A liquid storage tank is arranged in the shell 1 and is communicated with an outlet of theenrichment module 25. The blood entering from the inlet of the microfluidic chip 2 finally flows into the liquid storage tank to form a liquid flow loop, so that the blood can be continuously detected in real time.

The upper computer is provided with a display for displaying the concentration of the heparin. Of course, a display may be directly provided on the housing 1, and the heparin concentration data may be displayed through the display.

And a battery 5 for providing power for the laser-induced fluorescence detection device 3, the Bluetooth module 4 and the controller is arranged in the shell 1. The battery 5 is a rechargeable battery, and a charging interface for charging the battery 5 is arranged on the shell 1.

While the invention has been described in connection with what is presently considered to be the most practical and preferred embodiment, it is to be understood that the invention is not to be limited to the disclosed embodiment, but on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.

Claims (8)

Translated fromChinese

1.一种用于检测血液肝素含量的检测器，其特征在于，包括外壳，设于外壳内的微流控芯片，用于检测血液中肝素浓度的激光诱导荧光检测装置，用于将肝素浓度数据发送到上位机的蓝牙模块，以及用于接收激光诱导荧光检测装置检测出的肝素浓度并将数据通过蓝牙模块发送到上位机的控制器；所述外壳上设有与微流控芯片的入口连通的微通道，所述微通道与静脉注射针头连通。所述微流控芯片对血液进行过滤、将血液与试剂混合反应、将混合物中荧光物质富集，所述激光诱导荧光检测装置检测微流控芯片出口端的血液中的肝素浓度。1. a detector for detecting blood heparin content, is characterized in that, comprises shell, is arranged in the microfluidic chip in the shell, is used to detect the laser-induced fluorescence detection device of heparin concentration in blood, is used for the heparin concentration A Bluetooth module for sending data to the host computer, and a controller for receiving the heparin concentration detected by the laser-induced fluorescence detection device and sending the data to the host computer through the Bluetooth module; the shell is provided with an entrance to the microfluidic chip A communicating microchannel that communicates with an IV needle. The microfluidic chip filters the blood, mixes the blood with the reagent, and enriches the fluorescent substance in the mixture, and the laser-induced fluorescence detection device detects the heparin concentration in the blood at the outlet end of the microfluidic chip.2.根据权利要求1所述的一种用于检测血液肝素含量的检测器，其特征在于，所述微流控芯片包括用于防止血液倒流的单向阀，用于控制血液流速的压电蠕动微泵，用于过滤掉血液中的血细胞的过滤模块，用于将内含的试剂与血浆中的肝素进行结合形成荧光物质的混合模块，以及有于提升荧光物质浓度的富集模块；所述单向阀的入口与微通道连通，所述激光诱导荧光检测装置检测出从富集模块出口流出的血液中的荧光颗粒数目。2. A detector for detecting blood heparin content according to claim 1, wherein the microfluidic chip comprises a one-way valve for preventing blood backflow, a piezoelectric A peristaltic micropump, a filter module for filtering out blood cells in blood, a mixing module for combining the contained reagent with heparin in plasma to form fluorescent substances, and an enrichment module for increasing the concentration of fluorescent substances; The inlet of the one-way valve is communicated with the microchannel, and the laser-induced fluorescence detection device detects the number of fluorescent particles in the blood flowing out from the outlet of the enrichment module.3.根据权利要求2所述的一种用于检测血液肝素含量的检测器，其特征在于，所述富集模块内设有渗透膜，血液在渗透膜作用下去除水和无机盐，使荧光物质浓度提高。3. A detector for detecting blood heparin content according to claim 2, characterized in that, a permeable membrane is arranged in the enrichment module, and the blood removes water and inorganic salts under the action of the permeable membrane, and makes the fluorescence Substance concentration increases.4.根据权利要求3所述的一种用于检测血液肝素含量的检测器，其特征在于，所述外壳内设有储液池，所述储液池与富集模块的出口连通。4 . The detector for detecting blood heparin content according to claim 3 , wherein a liquid storage tank is arranged in the casing, and the liquid storage tank is communicated with the outlet of the enrichment module. 5 .5.根据权利要求1所述的一种用于检测血液肝素含量的检测器，其特征在于，所述上位机设有用于显示肝素浓度的显示器。5 . The detector for detecting heparin content in blood according to claim 1 , wherein the upper computer is provided with a display for displaying the heparin concentration. 6 .6.根据权利要求1所述的一种用于检测血液肝素含量的检测器，其特征在于，所述外壳内设有用于为激光诱导荧光检测装置、蓝牙模块和控制器提供电源的电池。6 . The detector for detecting blood heparin content according to claim 1 , wherein a battery for providing power for the laser-induced fluorescence detection device, the Bluetooth module and the controller is provided in the casing. 7 .7.根据权利要求5所述的一种用于检测血液肝素含量的检测器，其特征在于，所述电池为可充电电池，所述外壳上设有为电池充电的充电接口。7 . The detector for detecting blood heparin content according to claim 5 , wherein the battery is a rechargeable battery, and a charging interface for charging the battery is provided on the casing. 8 .8.根据权利要求1至6任一项所述的一种用于检测血液肝素含量的检测器，其特征在于，所述控制器为单片机。8 . The detector for detecting blood heparin content according to claim 1 , wherein the controller is a single-chip microcomputer. 9 .

Images