CN112690786A - Sugar tolerance test system and method thereof - Google Patents

Abstract

The invention relates to a sugar tolerance test system and a method thereof, belonging to the technical field of animal experiment equipment. Comprises a single mouse fixing cage, a control panel, a blood sugar measuring instrument and a computer host; a control panel is arranged on one end face of the single mouse fixing cage, and a fixing through hole for fixing the tail of the mouse is formed in the control panel; the control panel is connected with the computer host; the blood sugar measuring instrument is connected with the computer host. The invention overcomes the problems which are easy to occur in the process of carrying out the sugar tolerance test in the prior art; the stress reaction of the mouse caused by repeated grabbing of the mouse is not needed, so that the test accuracy is improved; the mouse is injected and blood is sampled and detected in sequence by the prompt of an injection information display on the control panel, so that the working strength and fatigue of an operator are relieved; the automatic generation of the blood glucose curve graph is convenient for data processing work of an operator, so that the detection result is timely and reliable.

Description

Sugar tolerance test system and method thereof

Technical Field

The invention relates to a sugar tolerance test system and a method thereof, belonging to the technical field of animal experiment equipment.

Background

The glucose tolerance test is commonly used in the research of metabolic diseases, and the test process generally involves measuring blood sugar of 15min,30min,45min,60min and 120min after intraperitoneal injection or intragastric glucose injection of experimental mice. Since blood glucose changes very rapidly, accurate blood drawing operation time of each mouse needs to be ensured during the test period, so that the measured blood glucose of the mouse only falls on the preset time node. However, in practice, a plurality of mice are often placed in a cage, and it takes time for laboratory personnel to grasp and distinguish the right mice and sequence, and a certain skill is required to control the time. Even single mouse single-cage storage can also lead to the test site demand grow, snatchs the mouse repeatedly moreover and can cause stress response to it, and then influences experimental accuracy. Therefore, there is a need in the art for a glucose tolerance test system that can draw blood from an experimental mouse in time without repeatedly grasping the mouse.

Disclosure of Invention

The invention aims to solve the technical problems that in a sugar tolerance test, a mouse does not need to be repeatedly grabbed, and the blood drawing detection of an experimental mouse can be timely and timely carried out.

In order to solve the problems, the technical scheme adopted by the invention is to provide a sugar tolerance test system, which comprises a single mouse fixing cage, a control panel, a blood sugar measuring instrument and a computer host; a control panel is arranged on one end face of the single mouse fixing cage, and a fixing through hole for fixing the tail of the mouse is formed in the control panel; the control panel is connected with the computer host; the blood sugar measuring instrument is connected with the computer host.

Preferably, the control panel is provided with an injection information display, a blood sugar information display, an injection button and a prompting lamp.

Preferably, the sugar tolerance test system comprises a plurality of relatively independent single mouse fixing cages, and the control panel is arranged on the end face of each single mouse fixing cage.

The invention provides a use method of a sugar tolerance test system, which comprises the following steps:

step 1: after experimental mice are fasted for 4 hours, the mice are respectively placed into a single mouse fixing cage for fixing, and the tail of the mouse is exposed outside the cage through a fixing through hole on a control panel; allowing the mice to calm for more than 30 min; inputting grouping information of a plurality of mice into a host computer; respectively measuring the blood glucose concentration of the initial 0min of the experiment;

step 2: from the first cage position, injecting 2g/kg of 20% glucose prepared by normal saline into the abdominal cavity of the experimental mouse, and simultaneously pressing a corresponding injection button on the cage position, and simultaneously starting timing by using an injection information display and a blood sugar information display; the injection information display prompts an operator to sequentially inject the mice according to the prompt through voice and a prompt lamp until the last mouse is injected;

and step 3: when the timer on the injection information display of the experimental mouse reaches the 15 th min,30min,60 min and 120min, the timer starts to count down, the red light flickers, and an operator is prompted by voice to prepare for measuring the blood sugar of the mouse;

and 4, step 4: the operator measures the blood sugar of each mouse through the blood sugar measuring instrument according to the prompt, and the blood sugar reading and the corresponding measuring time are simultaneously input into the computer host;

and 5: the computer host automatically generates a blood sugar curve graph according to the input grouping information and the blood sugar value.

Compared with the prior art, the invention has the following beneficial effects:

the invention overcomes the problems which are easy to occur in the process of carrying out the sugar tolerance test in the prior art; the stress reaction of the mouse caused by repeated grabbing of the mouse is not needed, so that the test accuracy is improved; the mouse is injected and blood is sampled and detected in sequence by the prompt of an injection information display on the control panel, so that the working strength and fatigue of an operator are relieved; the automatic generation of the blood glucose curve graph is convenient for data processing work of an operator, so that the detection result is timely and reliable.

Drawings

FIG. 1 is a schematic diagram of a single mouse immobilization cage according to the present invention;

FIG. 2 is a schematic representation of a plurality of mouse stationary cage combinations of the present invention;

reference numerals: 1. single mouse immobilization cage; 2. a control panel; 3. a fixing through hole; 4. the tail of the mouse; 5. an injection information display; 6. a blood glucose information display; 7. an injection button; 8. a warning light; 9. blood sugar measuring instrument.

Detailed Description

In order to make the invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings:

as shown in fig. 1-2, the present invention provides a sugar tolerance test system, comprising a singlemouse fixing cage 1, acontrol panel 2, a bloodsugar measuring instrument 9 and a computer host; acontrol panel 2 is arranged on one end face of the singlemouse fixing cage 1, and a fixing throughhole 3 for fixing thetail 4 of the mouse is arranged on thecontrol panel 2; thecontrol panel 2 is connected with a computer host; the bloodsugar measuring instrument 9 is connected with the computer host. Thecontrol panel 2 is provided with aninjection information display 5, a bloodsugar information display 6, aninjection button 7 and a promptinglamp 8. The sugar tolerance test system comprises a plurality of relatively independent singlemouse fixing cages 1, and acontrol panel 2 is arranged on the end face of each single mouse fixing cage.

The invention provides a use method of a sugar tolerance test system, which comprises the following steps:

step 1: after experimental mice are fasted for 4 hours, the mice are respectively placed into a singlemouse fixing cage 1 for fixing, and the tail of the mouse is exposed outside the cage through a fixing throughhole 4 on a control panel; allowing the mice to calm for more than 30 min; inputting grouping information of a plurality of mice into a host computer; respectively measuring the blood glucose concentration of the initial 0min of the experiment;

step 2: from the first cage position, injecting 2g/kg of 20% glucose prepared by normal saline into the abdominal cavity of the experimental mouse, simultaneously pressing acorresponding injection button 7 on the cage position, and simultaneously starting timing by aninjection information display 5 and a bloodglucose information display 6; the injection information display 5 prompts an operator to sequentially inject the mice according to the prompt through voice and aprompt lamp 8 until the last mouse is injected;

and step 3: when the timer on the injection information display 5 of the experimental mouse reaches the 15 th min,30min,60 min and 120min, the timer starts to count down, the red light flickers, and an operator is prompted by voice to prepare for measuring the blood sugar of the mouse;

and 4, step 4: the operator measures the blood sugar of each mouse through the bloodsugar measuring instrument 9 according to the prompt, and the blood sugar reading and the corresponding measuring time are simultaneously input into the computer host;

and 5: the computer host automatically generates a blood sugar curve graph according to the input grouping information and the blood sugar value.

Example (b):

the following explanations are: the procedure for sugar tolerance testing of 15 mice at a time is assumed by the present invention:

step 1: after experimental mice are fasted for 4 hours, the mice are respectively placed in fixed cage positions, the mice are allowed to be quiet for more than 30min, and mouse grouping information is input to a host in advance; respectively measuring the blood glucose concentration of the initial 0min of the experiment;

step 2: injecting 20% glucose injection 2g/kg prepared by normal saline into the abdominal cavity of the mouse from the first cage position, simultaneously pressing an injection button corresponding to the cage position, and simultaneously starting timing by using an injection information display and a blood sugar information display;

an injection information display: the time is reported every 1 minute (the countdown is started 10 seconds in advance, the mth time voice prompt is that the (m + 1) th mouse is ready to be injected), and simultaneously, the red light on the cage position of the (m + 1) th mouse flickers

And step 3: the operator continues to inject the (m + 1) th mouse according to the voice prompt until the last mouse;

and 4, step 4: when the timer of the No. m mouse reaches the 15 th min,30min,60 min and 120min, the countdown is started 10 seconds in advance, the red light flickers, and the voice prompt is as follows: the m-th mouse is ready for blood glucose measurement.

And 5: the operator measures the blood glucose of each mouse according to the prompt, and the blood glucose reading and the corresponding measuring time are simultaneously input into the host.

Step 6: the computer host automatically generates a blood glucose graph based on the input grouping information and the blood glucose values.

If the number N of the mice is large, the prompting interval time of the injection information display needs to be shortened, and the total time of the interval time N is less than 15min so as to avoid that all the measurements cannot be completed on time.

The invention may also be applied to rats, or to insulin resistance tests.

While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.

Claims (4)

Translated fromChinese

1.一种糖耐受试验系统，其特征在于：包括单个小鼠固定笼、控制面板、血糖测量仪和计算机主机；单个小鼠固定笼一端面上设有控制面板，控制面板上设有用于固定小鼠尾部的固定通孔；控制面板与计算机主机连接；血糖测量仪和计算机主机连接。1. a sugar tolerance test system, is characterized in that: comprise single mouse fixed cage, control panel, blood glucose measuring instrument and computer mainframe; Single mouse fixed cage end face is provided with control panel, and the control panel is provided with for The fixed through hole at the tail of the mouse is fixed; the control panel is connected with the computer host; the blood glucose meter is connected with the computer host.2.如权利要求1所述的一种糖耐受试验系统，其特征在于：所述控制面板上设有注射信息显示器、血糖信息显示器、注射按钮和提示灯。2 . The glucose tolerance test system according to claim 1 , wherein the control panel is provided with an injection information display, a blood glucose information display, an injection button and a prompt light. 3 .3.如权利要求2所述的一种糖耐受试验系统，其特征在于：所述糖耐受试验系统包括多个相对独立的单个小鼠固定笼，单个小鼠固定笼端面上设有所述控制面板。3. a kind of sugar tolerance test system as claimed in claim 2 is characterized in that: described sugar tolerance test system comprises a plurality of relatively independent single mouse fixed cages, and the end face of single mouse fixed cage is provided with all control panel.4.一种糖耐受试验系统使用方法，其特征在于：包括以下步骤：4. a method for using a glucose tolerance test system, characterized in that: comprise the following steps:步骤1：实验用小鼠禁食4小时后，将小鼠分别放入单个小鼠固定笼固定，并使小鼠尾巴通过控制面板上的固定通孔暴露在笼外；让小鼠安静30min以上；将多个小鼠的分组信息输入到计算机主机；再分别测量实验起始0min血糖浓度；Step 1: After the experimental mice were fasted for 4 hours, the mice were placed in a single mouse fixed cage for fixation, and the mouse tail was exposed to the outside of the cage through the fixed through hole on the control panel; let the mice be quiet for more than 30 minutes ; Input the grouping information of multiple mice into the computer host; and then measure the blood glucose concentration at the beginning of the experiment respectively;步骤2：从第一个笼位开始，给实验小鼠腹腔注射生理盐水配制的20％的葡萄糖2g/kg，同时按下笼位上对应的注射按钮，注射信息显示器和血糖信息显示器同时开始计时；注射信息显示器通过语音和提示灯提示操作者根据提示依次注射小鼠，直至最后一只；Step 2: From the first cage position, inject 20% glucose 2g/kg prepared with normal saline into the abdominal cavity of the experimental mice, press the corresponding injection button on the cage position at the same time, the injection information display and the blood glucose information display start timing at the same time ;The injection information display prompts the operator to inject the mice in sequence according to the prompts until the last one;步骤3：当实验小鼠的注射信息显示器上计时器到达第15min，30min，60min，120min时，计时器开始倒计时，红灯闪烁，并通过语音提示操作者准备测量小鼠血糖；Step 3: When the timer on the injection information display of the experimental mouse reaches the 15th, 30th, 60th, and 120th minutes, the timer starts to count down, the red light flashes, and the operator is prompted to measure the blood sugar of the mouse by voice;步骤4：操作者根据提示，通过血糖测量仪测量每只小鼠的血糖，血糖读数和对应的测量时间会同时输入计算机主机；Step 4: The operator measures the blood glucose of each mouse through the blood glucose meter according to the prompt, and the blood glucose reading and the corresponding measurement time will be input into the computer host at the same time;步骤5：计算机主机根据输入的分组信息以及血糖值自动生成血糖曲线图。Step 5: The computer host automatically generates a blood sugar curve graph according to the input grouping information and the blood sugar value.

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