CN112630215A - Multi-reaction-cup continuous measurement luminescence measurement device and luminescence measurement method - Google Patents

Abstract

The application relates to a multi-reaction cup continuous measurement luminescence measurement device, comprising: the device comprises a measuring chamber, a driving unit for driving a turntable in the measuring chamber to rotate, a door opening and closing unit for opening and closing an opening on a cover plate of the measuring chamber, a light-emitting measuring unit and a liquid injection and absorption unit; the liquid injection and suction unit comprises a bracket, a driving component and a needle component, the needle component comprises a liquid suction needle, a liquid injection needle and a needle fixing plate, and the liquid suction needle and the liquid injection needle respectively penetrate through the measuring chamber cover plate and correspond to reaction cups on the liquid suction level and the liquid injection level; the driving assembly comprises a screw motor, a transmission screw, a screw nut and a driving seat which are fixedly connected to the needle fixing plate, a linear guide rail arranged on the support and a sliding block which is fixed on the driving seat and matched with the linear guide rail, one end of the transmission screw is connected with an output shaft of the screw motor, and the other end of the transmission screw is connected with a cover plate of the measuring chamber through a bearing. The application also relates to a luminescence measurement method, which saves the test time and realizes the dry-wet separation treatment of the waste liquid and the used reaction cup.

Description

Multi-reaction-cup continuous measurement luminescence measurement device and luminescence measurement method

Technical Field

The application belongs to the technical field of medical instruments, and particularly relates to a multi-reaction-cup continuous measurement luminescence measurement device and a luminescence measurement method.

Background

Currently, the most commonly used diagnostic method in the medical field, in vitro diagnosis, refers to collecting body fluids, excretions, and secretions of a human body to perform chemical composition or chemical reaction analysis, so as to determine human body lesions, such as chemiluminescence immunoassay, molecular diagnostics, and the like, wherein the diagnostic methods all use automatic or semi-automatic instruments to sample and analyze samples and give diagnostic reports, and the instruments all have a functional unit, namely a luminescence value detection unit (i.e., a luminescence measurement device).

The luminescence measuring device is a key functional unit which influences the accuracy of the measuring result and the testing speed of a chemiluminescence immunoassay analyzer, for example, the measuring device provided by the patent (document No. CN107167427A) of my application, namely a single reaction cup luminescence measuring device, can only carry out single reaction cup measurement, after the measurement is finished, a sealed bin gate is opened to take out the reaction cup, and then the next test is carried out, so that when one reaction cup of a measuring chamber is used for measuring, other reaction cup stations are in a waiting state, and the testing speed of the instrument is greatly reduced; meanwhile, after the luminescence measurement is finished, the waste liquid and the used reaction cup are not respectively treated, so that the post-treatment cost and time are greatly improved.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the light-emitting measuring device and the light-emitting measuring method for the multi-reaction-cup continuous measurement are provided for solving the technical problems that the reaction-cup light-emitting measuring device and the light-emitting measuring method in the prior art are low in testing speed and high in post-treatment cost of a used reaction cup.

The technical scheme adopted by the invention for solving the technical problems is as follows:

a multi-cuvette continuous-measurement luminescence measurement apparatus, comprising:

the measuring chamber is provided with a measuring chamber cover plate with an opening at the top, photon through holes are formed in the side surface of the measuring chamber, an accommodating cavity for accommodating a turntable is formed in the measuring chamber, a plurality of reaction cup accommodating positions which are arranged along the circumferential direction of the turntable and are used for accommodating reaction cups are formed in the turntable, and the opening is used for placing the reaction cups into the reaction cup accommodating positions or taking the reaction cups away from the reaction cup accommodating positions;

the driving unit is used for driving the turntable to rotate so that the reaction cups sequentially flow among different stations of the reaction cup taking and placing position, the shaking position, the liquid injection or luminescence measuring position and the liquid absorption position;

a door opening and closing unit for opening or closing an opening on the cover plate of the measuring chamber;

the shaking unit is used for carrying out shaking action on the reaction cups which are transferred to the shaking position;

the light-emitting measurement unit can close or open the photon via hole on the measurement chamber and collect the light-emitting quantity of the substance in the reaction cup on the liquid injection or light-emitting measurement position when the photon via hole is opened;

the liquid injection and absorption unit is used for injecting liquid into the reaction cup corresponding to the liquid injection position and extracting waste liquid from the reaction cup corresponding to the liquid absorption position, and comprises a bracket, a driving component and a needle component which are fixed on a cover plate of the measuring chamber; the needle assembly comprises a liquid suction needle, a liquid injection needle and a needle fixing plate fixedly connected with the liquid suction needle and the liquid injection needle and positioned at the upper part of the measuring chamber cover plate, the liquid suction needle and the liquid injection needle respectively penetrate through the measuring chamber cover plate and correspond to reaction cups on the liquid suction level and the liquid injection level, and the needle point of the liquid suction needle and the needle point of the liquid injection needle have a certain height difference; the drive assembly includes screw motor, transmission lead screw, in on the transmission lead screw spiral drive link firmly lead screw nut on the needle fixed plate, be fixed in the drive seat on the needle fixed plate, set up on the support with the linear guide that the transmission lead screw is parallel and be fixed in on the drive seat with linear guide complex slider, the output shaft of screw motor is connected to transmission lead screw one end, and the other end passes through the bearing and is connected with measurement chamber apron, can make through screw motor, transmission lead screw be fixed in the imbibition needle on the needle fixed plate, annotate the liquid needle and reciprocate to through needle fixed plate, drive seat drive slider along linear guide reciprocate.

Preferably, annotate liquid and imbibition unit still includes annotate liquid needle seal assembly and imbibition needle seal assembly, annotate liquid needle seal assembly and include: the sealing column cover plate, the first sealing plug and the sealing column are provided with through holes for the liquid injection needle to pass through, and the bottom of the sealing column is provided with an accommodating cavity with the diameter larger than the outer diameter of the liquid injection needle; the pipette needle seal assembly comprises: the second sealing plug is arranged in the groove at the top of the measuring chamber cover plate and connected with the sealing plug cover plate arranged on the measuring chamber cover plate, and the second O-shaped ring is arranged on the periphery of the second sealing plug; preferably, interference fit is adopted between the liquid injection needle and the first sealing plug, between the liquid suction needle and the second sealing plug, between the first O-shaped ring and the first sealing plug, and between the second O-shaped ring and the second sealing plug, tapered hole structures are preferably adopted in inner holes of the first sealing plug and the second sealing plug, and wear-resistant black plastics are preferably adopted in the first sealing plug and the second sealing plug.

Preferably, the support consists of a top plate, a vertical plate and a base, the base is fixed on a cover plate of the measuring chamber, and the linear guide rail is arranged on the vertical plate; the upper end and the lower end of the vertical plate are preferably provided with two limiting pins for limiting the sliding block.

Preferably, the driving seat is provided with an optical coupler catch, and the vertical plate is provided with an optical coupler corresponding to the optical coupler catch; before the turntable rotates, a signal is sent to a control system through the induction of the optical coupler separation blade and the optical coupler, and the resetting of the whole needle assembly is realized; preferably, the liquid suction needle is connected with a waste liquid pipe, the waste liquid pipe is connected with a waste liquid pump, the liquid injection needle is connected with a liquid injection pipe, and the liquid injection pipe is connected with a liquid injection pump; the liquid suction needle tip and the liquid injection needle tip preferably have a certain height difference.

Preferably, the driving unit comprises a rotary power mechanism and a rotary transmission mechanism, the rotary transmission mechanism comprises a rotating shaft, a driving synchronous pulley and a driven synchronous pulley which are connected through a synchronous belt, one end of the rotating shaft is fixedly connected with the turntable through a bearing, the other end of the rotating shaft is connected with the driven synchronous pulley through a bearing and a shaft collar, and the driving synchronous pulley is fixedly connected with the rotary power mechanism; preferably, the drive unit still includes canceling release mechanical system, canceling release mechanical system includes the opto-coupler that resets and installs in driven synchronous pulley bottom and the opto-coupler separation blade that resets, and the opto-coupler that resets is fixed in the measuring room through the opto-coupler support that resets on, before the official test, through the response of the opto-coupler that resets and the opto-coupler separation blade that resets to send signal to control system, make each reaction cup holding position fix a position to initial position.

Preferably, the door opening and closing unit comprises a seat body, a door driving motor and a transmission screw rod, wherein the door, the door driving motor and the transmission screw rod are installed on the seat body, one end of the transmission screw rod is connected with an output shaft of the driving motor, the other end of the transmission screw rod is connected with the door, a door opening is formed in the seat body, the door opening corresponds to an opening of the measuring chamber cover plate, guide rails are arranged on two sides of the seat body, and the door can be driven to slide above.

Preferably, the light-emitting measurement unit comprises a photomultiplier, a rotary driving piece arranged on the mounting plate, and a baffle plate with a light through hole, wherein a chute is formed on the baffle plate, a rotary rocker positioned on the cam and driven by the rotary driving piece to rotate is arranged in the chute, and when the rotary rocker rotates, the baffle plate horizontally moves through the cooperation with the chute, so that the through hole on the baffle plate is overlapped or dislocated with the photon through hole; preferably, the rotary driving piece includes rotary driving motor and drive shaft, drive shaft one end is connected drive motor's output shaft, the cam is connected to the other end, be provided with the location opto-coupler that opens the door in the both sides of drive shaft, the location opto-coupler that closes the door, and fixed being provided with in the drive shaft with the location opto-coupler that opens the door, the corresponding location opto-coupler separation blade that has the open-ended of location opto-coupler that closes the door, the location opto-coupler that opens the door, the location opto-coupler that closes the door, location opto-coupler separation blade is used for responding to whether the photon via hole is sheltered from.

Preferably, the shaking unit comprises an up-and-down displacement component and a shaking component, the up-and-down displacement component is used for controlling the lifting of the reaction cup, and when the up-and-down displacement component drives the reaction cup to lift, the shaking component can drive the reaction cup to eccentrically rotate; preferably, the up-down displacement assembly comprises a lifting power mechanism, a horizontally arranged moving support plate, a vertically arranged limit plate and a linear guide rail arranged on the limit plate, wherein the lifting power mechanism drives the moving support plate to move up and down along the linear guide rail; preferably, the shaking component comprises a shaking power mechanism and an extension rod, the shaking power mechanism is connected with the movable support plate, one end of the extension rod is fixedly connected with the shaking power mechanism, the other end of the extension rod is fixedly connected with an eccentric block, the eccentric block can slide upwards along with the movable mechanism to deeply enter the reaction cup containing position, and jacks up a reaction cup located in the reaction cup containing position to abut against a reaction cup limiting mechanism located above the shaking position, so that the reaction cup is clamped and fixed by the reaction cup limiting mechanism located above and the eccentric block located below.

The invention also provides a multi-reaction-cup continuous measurement luminescence measurement method, which is completed by adopting the multi-reaction-cup continuous measurement luminescence measurement device in any embodiment, and comprises the following steps:

s1: starting a door opening and closing unit to open an opening of a cover plate of the measuring chamber, executing reaction cup taking-away action on a reaction cup taking and placing position, placing a reaction cup filled with a buffer solution 1 into a reaction cup containing position corresponding to the reaction cup taking and placing position, and then closing the opening of the cover plate of the measuring chamber through the door opening and closing unit;

s2: the driving unit drives the rotary table to rotate, so that reaction cups in the reaction cup containing positions sequentially flow among different stations of a reaction cup taking and placing position, a shaking position, a liquid injection level and a liquid absorption position, the shaking unit is used for shaking up the reaction cups which are rotated to the shaking position, abuffer solution 2 is injected into the reaction cups which are rotated to the liquid injection position through the liquid injection and absorption unit, a photon through hole is opened through the light emitting measurement unit so as to collect the light emitting quantity of substances in the reaction cups, the liquid absorption unit is used for absorbing liquid of waste liquid in the reaction cups which are rotated to the liquid absorption position, the reaction cup taking-away action is carried out on the reaction cups which are rotated to the reaction cup taking and placing positions, and new reaction cups are placed on the reaction cup taking and placing positions; the liquid injection or luminescence measurement action or the placing or taking-away action of the reaction cup can be simultaneously carried out with the shaking and liquid absorption actions of the reaction cup respectively, the liquid injection or luminescence measurement action and the placing or taking-away action of the reaction cup can not be simultaneously carried out, the opening of the cover plate of the measurement chamber is closed when the liquid injection or luminescence measurement is carried out, the photon through hole on the measurement chamber is closed through the luminescence measurement unit when the placing or taking-away action of the reaction cup is carried out, and the photomultiplier is prevented from being damaged by exposure.

Preferably, the luminescence measuring unit is started before liquid injection is carried out through the liquid injection and absorption unit; preferably, during liquid injection and liquid suction, the liquid suction needle and the liquid injection needle are driven by the screw rod motor to slowly descend, liquid suction is always performed in the descending process until the liquid suction needle touches the bottom of the reaction cup, liquid injection is started when the needle point of the liquid injection needle enters the reaction cup, and the liquid injection needle cannot touch the buffer liquid in the reaction cup when the liquid suction needle touches the bottom of the reaction cup.

The invention has the beneficial effects that:

the reaction cup luminescence measuring device and the luminescence measuring method can process luminescence measurement of a plurality of reaction cups in parallel, and liquid injection or luminescence measuring actions or placing or taking actions of the reaction cups can be carried out simultaneously with shaking and liquid absorption actions of the reaction cups respectively by enabling the reaction cups in the reaction cup containing positions to sequentially circulate among different stations of the reaction cup taking and placing positions, the shaking position, the liquid injection position and the liquid absorption position, so that the test time is greatly saved; annotate liquid and imbibition unit and have liquid and imbibition function concurrently, accomplish luminous measurement back atinjection buffer solution 2, can make the reaction cup shift to next station (inhale the liquid level) to directly get rid of the waste liquid of reaction cup outside the system and retrieve centralized processing, later can shift the reaction cup to the reaction cup of no waste liquid and take away the reaction cup of taking away again next station, realized the wet-dry separation processing of waste liquid and used reaction cup, degree of automation is high.

Drawings

The technical solution of the present application is further explained below with reference to the drawings and the embodiments.

FIG. 1 is a schematic view of the overall structure of a multi-cuvette continuous measurement luminescence measurement apparatus according to example 1 of the present application;

FIG. 2 is a schematic view showing the structure of a measuring chamber (with a measuring chamber cover plate) according to example 1 of the present application;

FIG. 3 is a schematic view showing the structure of a measuring chamber (without a cover plate of the measuring chamber) according to example 1 of the present application;

fig. 4 is a schematic structural view of a drive unit of embodiment 1 of the present application;

fig. 5 is a schematic structural diagram of a door opening and closing unit according to embodiment 1 of the present application;

FIG. 6 is a schematic structural view of a luminescence measurement unit according to embodiment 1 of the present application;

FIG. 7 is a sectional view of a luminescence measurement unit according to embodiment 1 of the present application;

fig. 8 is a schematic structural view of a positioning optical coupling baffle in embodiment 1 of the present application;

FIG. 9 is a schematic structural view of a shake-up unit of embodiment 1 of the present application;

fig. 10 and 11 are schematic structural views of a liquid injection and suction unit according to example 1 of the present application;

FIG. 12 is a sectional view of a liquid injection and suction unit according to example 1 of the present application;

the reference numbers in the figures are: 1-measuring chamber, 101-measuring chamber cover plate, 102-rotating disc, 1021-reaction cup containing position, 103-fixed flange, 2-driving unit, 201-driving motor, 202-rotary transmission mechanism bracket, 203-fixed column, 204-driving synchronous pulley, 205-synchronous belt, 206-driven synchronous pulley, 207-reset optical coupler bracket, 208-reset optical coupler, 209-reset optical coupler baffle, 210-bearing, 211-rotating shaft, 3-door opening and closing unit, 301-seat body, 302-door, 303-door driving motor, 304-driving screw rod, 4-luminescence measuring unit, 401-photomultiplier, 402-baffle, 4021-sliding chute, 4022-light through hole, 403-cam, 4031-rotating rocker, 404-a rotary driving piece, 4041-a rotary driving motor, 4042-a driving shaft, 405-a mounting plate, 406-a positioning optical coupler baffle, 4061-a notch, 407-a door opening positioning optical coupler, 408-a door closing positioning optical coupler, 5-a shaking unit, 501-a lifting power mechanism, 502-a moving support plate, 503-a limiting plate, 504-a shaking power mechanism, 505-an extension rod, 506-an eccentric block, 6-a liquid injection and absorption unit, 601-a support, 6011-a top plate, 6012-a vertical plate, 6013-a base, 602-a driving component, 6021-a lead screw motor, 6022-a transmission lead screw, 6023-a driving seat, 6024-an optical coupler baffle, 6025-an optical coupler, 6026-a sliding block, 6027-a linear guide rail, 6028-a limiting pin and 603-a needle component, 6031-needle fixing plate, 6032-liquid suction needle, 6033-liquid injection needle, 604-liquid injection needle sealing component, 6041-first O-shaped ring, 6042-first sealing plug, 6043-sealing column, 6044-sealing column cover plate, 605-liquid suction needle sealing component, 6051-sealing plug cover plate, 6052-second sealing plug and 6053-second O-shaped ring.

Detailed Description

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and are not to be considered limiting of the scope of the present application. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art through specific situations.

The technical solutions of the present application will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

Example 1

The present embodiment provides a luminescence measurement apparatus for continuous measurement with multiple cuvettes, as shown in fig. 1, including:

as shown in fig. 2 and 3, the measuring chamber 1 has ameasuring chamber cover 101 with an opening at the top, a photon via hole at the side, and a containing cavity for containing theturntable 102 inside, wherein the opening is used for placing a reaction cup into a reactioncup containing position 1021 or taking the reaction cup away from the reactioncup containing position 1021, and theturntable 102 is provided with a plurality of reactioncup containing positions 1021 arranged along the circumferential direction of theturntable 102 for containing the reaction cup;

thedriving unit 2, as shown in fig. 4, is used for driving theturntable 102 to rotate, and includes a rotation power mechanism and a rotation transmission mechanism, and the rotation transmission mechanism is connected to theturntable 102 of the measuring chamber 1; the rotary power mechanism adopts adriving motor 201 and is fixed on the measuring chamber 1 through a rotarytransmission mechanism bracket 202 and afixed column 203 arranged on the rotarytransmission mechanism bracket 202; the rotary transmission mechanism comprises arotary shaft 211, a drivingsynchronous pulley 204 and a drivensynchronous pulley 206 which are connected through asynchronous belt 205, one end of therotary shaft 211 is fixedly connected with theturntable 102 through abearing 210, the other end of the rotary shaft is connected with the drivensynchronous pulley 206 through thebearing 210 and ashaft collar 214, and the drivingsynchronous pulley 204 is fixedly connected with a rotary power mechanism; thebearing 210 is arranged in a bearing hole at the bottom of the measuring chamber, and the rotatingshaft 211 is connected with theturntable 102 through afixed flange 103 on theturntable 102; when thedriving unit 2 drives theturntable 102 to rotate, the reaction cups can successively flow among different stations of a reaction cup taking and placing position, a shaking position, a liquid injection or luminescence measuring position and a liquid absorption position, and a series of actions of reaction cup placing or taking, shaking, liquid injection or luminescence measuring, liquid absorption and the like are respectively realized; thedriving unit 2 further comprises a reset mechanism, the reset mechanism comprises a resetoptical coupler 208 and a reset opticalcoupler blocking piece 209 which is arranged at the bottom of the drivensynchronous pulley 206 and corresponds to the resetoptical coupler 208, the resetoptical coupler 208 is fixed on the measuring room through a resetoptical coupler support 207, and before formal testing, signals are sent to a control system through the induction of the resetoptical coupler 208 and the reset opticalcoupler blocking piece 209, so that each reaction cupaccommodating position 1021 is positioned to an initial position;

a door opening andclosing unit 3, as shown in fig. 5, provided on themeasurement chamber cover 101 for opening or closing an opening in themeasurement chamber cover 101; the door opening andclosing unit 3 comprises aseat body 301, adoor 302, adoor driving motor 303 and atransmission screw rod 304, wherein thedoor 302, thedoor driving motor 303 and thetransmission screw rod 304 are installed on theseat body 301, one end of thetransmission screw rod 304 is connected with an output shaft of thedriving motor 303, the other end of the transmission screw rod is connected with thedoor 302, a door opening is formed in theseat body 301 and corresponds to an opening of the measuringchamber cover plate 101, guide rails are arranged on two sides of theseat body 301, and thedoor driving motor 303 can drive the door; when the door needs to be closed, thedriving motor 303 can be operated to enable thecabin door 302 to slide above the door opening until the door opening is covered; when the door needs to be opened and the sample is added, the drivingmotor 303 can also be operated to enable thecabin door 302 to slide away from the upper part of the door opening;

the luminescence measurement unit 4, as shown in fig. 6, 7, and 8, can close or open a photon via hole on the measurement chamber 1, and collect the luminescence amount of the substance in the reaction cup on the injection or luminescence measurement site when opening the photon via hole, and includes aphotomultiplier 401, arotary drive 404 mounted on themounting plate 405, and abaffle 402 having a light throughhole 4022, where thebaffle 402 is further formed with achute 4021, arotary rocker 4031 on thecam 403, driven to rotate by therotary drive 404, is disposed in thechute 4021, and when therotary rocker 4031 rotates, thebaffle 402 is horizontally moved by matching with thechute 4021, so that thethrough hole 4022 on thebaffle 402 coincides with the photon via hole (thebaffle 402 does not block photons) or is misaligned (thebaffle 402 blocks photons); when the photoelectric detector is used, before thecabin door 302 is opened, thebaffle 402 is driven to move by therotary driving piece 404, so that the light throughhole 4022 on thebaffle 402 is staggered with the photon through hole, the lens of thephotomultiplier 401 can be shielded, external light is prevented from being emitted into thephotomultiplier 401, and the service life and the precision of thephotomultiplier 401 are ensured; after thehatch door 302 is closed, thebaffle 402 is driven to move by therotary driving piece 404, so that the light throughhole 4022 on thebaffle 402 is overlapped with the photon through hole, and the light emitting quantity of the substances in the reaction cup can be acquired by thephotomultiplier tube 401; further, therotary driving member 404 includes arotary driving motor 4041 and adriving shaft 4042, one end of thedriving shaft 4042 is connected to an output shaft of thedriving motor 4041, the other end is connected to thecam 403, a dooropening positioning optocoupler 407 and a doorclosing positioning optocoupler 408 are disposed on two sides of thedriving shaft 4042, and apositioning optocoupler catch 406 having an opening and corresponding to the dooropening positioning optocoupler 407 and the doorclosing positioning optocoupler 408 is fixedly disposed on the driving shaft 412, the dooropening positioning optocoupler 407, the doorclosing positioning optocoupler 408 and thepositioning optocoupler catch 406 are used for sensing whether the photon via hole is blocked by thebaffle 402, and sending a signal to the control system to open or close the opening of the measurement chamber cover; when thenotch 4061 on the positioningoptical coupler catch 406 is aligned with the door-opening positioningoptical coupler 407, the lens of thephotomultiplier tube 401 is aligned with the light-passing hole 4022, and at this time, the hatchdoor driving assembly 303 is prevented from opening the opening of themeasurement chamber cover 101, so that thephotomultiplier tube 401 is prevented from being damaged due to irradiation of external light; when thenotch 4061 on thepositioning optocoupler catch 406 is aligned with the door-closing positioning optocoupler 408, the lens of thephotomultiplier tube 401 is shielded by thebaffle 402, and at this time, the hatchdoor driving assembly 303 can start to open the opening of themeasurement chamber cover 101;

theshaking unit 5 is used for shaking the reaction cup which is transferred to the shaking position, and comprises an up-and-down displacement component and a shaking component, wherein the up-and-down displacement component is used for controlling the lifting of the reaction cup, and when the up-and-down displacement component drives the reaction cup to lift, the shaking component can drive the reaction cup to eccentrically rotate; the up-down displacement assembly comprises alifting power mechanism 501, a horizontally arranged movingsupport plate 502, a vertically arrangedlimiting plate 503 and a linear guide rail arranged on thelimiting plate 503, wherein thelifting power mechanism 501 drives themoving support plate 502 to move up and down along the linear guide rail; the shaking-up component comprises a shaking-up power mechanism 504 and anextension rod 505, the shaking-up power mechanism 502 is connected with themovable support plate 502, one end of theextension rod 505 is fixedly connected with the shaking-uppower mechanism 502, the other end of the extension rod is fixedly connected with aneccentric block 506, theeccentric block 506 can slide upwards along with themovable mechanism 502 to deeply extend into the reactioncup accommodating position 1021, and the reaction cup positioned in the reactioncup accommodating position 1021 is jacked up to be abutted against a reaction cup limiting mechanism positioned above the shaking-up position, so that the reaction cup is clamped and fixed by the reaction cup limiting mechanism positioned above and theeccentric block 506 positioned below to ensure the stability during eccentric rotation;

the liquid injection andsuction unit 6 is used for injecting liquid into the reaction cup corresponding to the liquid injection position and extracting waste liquid from the reaction cup corresponding to the liquid suction position, and as shown in fig. 10, comprises abracket 601, adriving assembly 602 and aneedle assembly 603; thesupport 601 consists of atop plate 6011, avertical plate 6012 and abase 6013, wherein thebase 6013 is fixed on the measuringchamber cover plate 101; theneedle assembly 603 comprises aliquid suction needle 6032, aliquid injection needle 6033 and aneedle fixing plate 6031 fixedly connected with theliquid suction needle 6031 and theliquid injection needle 6033 and positioned at the upper part of the measuringchamber cover plate 101, theliquid suction needle 6032 and theliquid injection needle 6033 respectively penetrate through the measuringchamber cover plate 101 and correspond to reaction cups at a liquid suction level and a liquid injection level, and the needle point of theliquid suction needle 6032 and the needle point of theliquid injection needle 6033 have certain height difference; furthermore, a notch is formed at the needle point of theliquid suction needle 6032, so that the needle port can be prevented from being blocked when the needle port of theliquid suction needle 6032 is attached to the bottom of the reaction cup, and theinjection needle 6033 is provided with a conical needle point 321, so that theexcitant injection needle 6033 can be prevented from hanging liquid; thedriving assembly 602 comprises ascrew rod motor 6021, atransmission screw rod 6022, ascrew rod nut 6034 which is screwed on thetransmission screw rod 6022 and fixedly connected to aneedle fixing plate 6031, adriving seat 6023 which is fixed on theneedle fixing plate 6031, alinear guide rail 6027 which is arranged on thevertical plate 6012 and is parallel to thetransmission screw rod 6022, and asliding block 6026 which is fixed on thedriving seat 6023 and is matched with thelinear guide rail 6027, wherein one end of thetransmission screw rod 6022 is connected with an output shaft of thescrew rod motor 6021, and the other end is connected with the measuringchamber cover plate 101 through a bearing, the liquid suckingneedle 6032 and theliquid injection needle 6033 which are fixed on theneedle fixing plate 6031 can move up and down through thescrew rod motor 6021 and thetransmission screw rod 6022, and the slidingblock 6026 is driven to move up and down along thelinear guide rail 6027 through theneedle fixing plate 6031;

annotate liquid andimbibition unit 6 still includes annotate liquidneedle seal assembly 604 and imbibitionneedle seal assembly 605, annotate liquidneedle seal assembly 604 and imbibitionneedle seal assembly 605 and be used for playing sealed effect when annotating the upper and lower operation ofliquid needle 6033 andimbibition needle 6032 to prevent the entering of external light, annotate liquidneedle seal assembly 604 and include: the sealing device comprises asealing column 6043 arranged on a measuringchamber cover plate 101, a sealingcolumn cover plate 6044 arranged at the top of thesealing column 6043, afirst sealing plug 6042 arranged in a groove at the top of thesealing column 6043 and connected with the sealingcolumn cover plate 6044, and a first O-shaped ring 6041 arranged on the periphery of thefirst sealing plug 6042, wherein a through hole for aliquid injection needle 6033 to pass through is formed in the sealingcolumn cover plate 6044, thefirst sealing plug 6042 and thesealing column 6043, and the bottom of thesealing column 6043 is provided with an accommodating cavity with a liquid injection diameter larger than the outer diameter of the needle; the pipetteneedle seal assembly 5 includes: asecond sealing plug 6052 arranged in a groove at the top of the measuringchamber cover plate 101 and connected with a sealingplug cover plate 6051 arranged on the measuringchamber cover plate 101, and a second O-shaped ring 6053 arranged on the periphery of thesecond sealing plug 6052, wherein through holes for theliquid suction needle 6032 to pass through are formed in the sealingplug cover plate 6051 and thesecond sealing plug 6052; annotate betweenliquid needle 6033 and thefirst sealing plug 6042, betweenimbibition needle 6032 and thesecond sealing plug 6052, between firstO shape circle 6041 and thefirst sealing plug 6042, all adopt interference fit between secondO shape circle 6053 and thesecond sealing plug 6052,first sealing plug 6042,second sealing plug 6052 hole adopts the bell mouth structure,first sealing plug 6042,second sealing plug 6052 further adopts wear-resisting black plastics, like black PTFE, black high rigidity, PU black UPE etc. can effectively avoid external light to get into the inside cavity of measuring chamber. In addition, the liquid injectionneedle sealing assembly 604 has a larger design of thesealing column 6043 than the liquidsuction needle assembly 605, mainly because the needle point of theliquid suction needle 6032 and the needle point of theliquid injection needle 6033 have a certain height difference, when theliquid suction needle 6032 returns to the home position, if the liquid injection needle sealing assembly is arranged at the measuringchamber cover plate 101 without thesealing column 6043, theliquid injection needle 6033 and the sealing assembly can be separated.

Further, twolimit pins 6028 are arranged at the upper end and the lower end of thevertical plate 6012 and are used for limiting theslide block 6026, that is, when the whole mechanism fails, theliquid suction needle 6032 and theliquid injection needle 6033 cannot move upwards or downwards without limitation, so as to avoid failure, such as puncturing a reaction cup.

Further, anoptocoupler baffle 6024 is mounted on thedriving seat 6023, and anoptocoupler 6025 corresponding to theoptocoupler baffle 6024 is mounted on thevertical plate 6012; before theturntable 102 rotates, the opticalcoupler retaining plate 6024 and theoptical coupler 6025 are used for sensing, so that a signal is sent to a control system, and thewhole needle assembly 603 is reset; when theneedle assembly 603 is at the home position, thepipette needle 6032 and thefilling needle 6033 are located above the cup hole (greater than 4mm) of the corresponding reaction cup, so that the needle tip interferes with the rotation of the reaction cup to cause a fault, and thepipette needle 6032, thefilling needle 6033 and the needle tip cannot be separated from the inner hole of thesealing plug 6042, so that thepipette needle 6032 and thefilling needle 6033 cannot align with the inner hole of thesealing plug 6042 when moving downwards again.

Further, theliquid suction needle 6032 is connected with a waste liquid pipe, and the waste liquid pipe is connected with a waste liquid pump; theliquid injection needle 6033 is connected with a liquid injection pipe, and the liquid injection pipe is connected with a liquid injection pump; during liquid injection and liquid suction, theliquid suction needle 6032 and theliquid injection needle 6033 are driven by thescrew rod motor 6021 to slowly descend, and in the descending process, the waste liquid pump is always in a working state until theliquid suction needle 6032 touches the bottom of the reaction cup, while the liquid injection pump starts to work when the needle point of theliquid injection needle 6033 enters the reaction cup 7, the excitant is injected into the reaction cup in the past, and when theliquid suction needle 6032 touches the bottom of the reaction cup, theliquid injection needle 6033 cannot touch the excitant in the cup.

During the use, when the reaction cup rotates to annotateliquid needle 6033 below, be in and annotate the liquid level, when the reaction cup rotates toimbibition needle 6032 below, be in and inhale the liquid level, in the continuous test, when the former reaction cup is in and inhales useless liquid level, the latter reaction cup is in and annotates the liquid level, inhales the waste liquid promptly and annotates the excitant and go on simultaneously, has so saved test time greatly.

Example 2

The embodiment provides a multi-cup continuous measurement luminescence measurement method, which is completed by adopting the multi-cup continuous measurement luminescence measurement apparatus of embodiment 1, and comprises the following steps:

s1: starting the door opening andclosing unit 3 to open the opening of thecover plate 101 of the measuring chamber 1, executing reaction cup taking action on the reaction cup taking and placing position (no reaction cup exists at the beginning of the test, and empty grabbing action is executed), placing the reaction cup filled with the buffer solution 1 into the reaction cupaccommodating position 1021 corresponding to the reaction cup taking and placing position, and then closing the opening of thecover plate 101 of the measuring chamber 1 through the door opening andclosing unit 3;

s2: the turntable 102 is driven to rotate by the driving unit 2, so that reaction cups in the reaction cup accommodating position 1021 sequentially flow among different stations of a reaction cup taking and placing position, a shaking position, a liquid injection position and a liquid absorption position, the shaking action is performed on the reaction cups which rotate to the shaking position by the shaking unit 4, buffer liquid 2 is injected into the reaction cups which rotate to the liquid injection position by the liquid injection and absorption unit 6, photon via holes are opened by the luminescence measurement unit 4 to collect the luminescence quantity of substances in the reaction cups, the liquid absorption action is performed on waste liquid in the reaction cups which rotate to the liquid absorption position by the liquid injection and absorption unit 6, the reaction cup taking action is performed on the reaction cups which rotate to the reaction cup taking and placing position, and new reaction cups are placed on the reaction cup taking and placing positions; the liquid injection or luminescence measurement action or the placing or taking-away action of the reaction cup can be simultaneously carried out with the shaking and liquid absorption actions of the reaction cup respectively, the liquid injection or luminescence measurement action and the placing or taking-away action of the reaction cup can not be simultaneously carried out, the opening of the cover plate 101 of the measurement chamber 1 is closed when the liquid injection or luminescence measurement is carried out, the phenomenon that external light enters the measurement chamber 1 to influence a luminescence measurement result is avoided, the photon through hole on the measurement chamber 1 is closed through the luminescence measurement unit 4 when the placing or taking-away action of the reaction cup is carried out, and the photomultiplier 401 is prevented from being exposed and damaged; when the turntable 102 rotates, the reaction cups should be placed or taken away, the reaction cups should be shaken up, the liquid injection or luminescence measurement and the liquid absorption should be stopped.

In the luminescence measurement method for continuous measurement of multiple cuvettes of the present embodiment, the luminescence measurement unit 4 is started before the liquid injection andabsorption unit 6 injects liquid, that is, the photon via hole and thephotomultiplier 401 are opened to collect the luminescence amount of the material in the cuvette, thereby preventing the collection of photons from being missed and improving the collection precision. In addition, the photon number is collected continuously to obtain the total number of photons in a period of time, and the precision is higher than that when the photon number is collected intermittently.

In the luminescence measurement method for continuous measurement of multiple reaction cups in this embodiment, during liquid injection and liquid suction, theliquid suction needle 6032 and theliquid injection needle 6033 are driven by thescrew motor 6021 to slowly descend, in the descending process, the waste liquid pump is always in a working state until theliquid suction needle 6032 touches the bottom of the reaction cup, while when the needle point of theliquid injection needle 6033 enters the reaction cup 7, the liquid injection pump starts working, the activator is injected into the reaction cup before, and when theliquid suction needle 6032 touches the bottom of the reaction cup, theliquid injection needle 6033 cannot touch the solution in the reaction cup.

In light of the foregoing description of the preferred embodiments according to the present application, it is to be understood that various changes and modifications may be made without departing from the spirit and scope of the invention. The technical scope of the present application is not limited to the contents of the specification, and must be determined according to the scope of the claims.

Claims (10)

1. A multi-cuvette continuous measurement luminescence measurement apparatus, comprising:

the measuring chamber comprises a measuring chamber (1), wherein the top of the measuring chamber is provided with a measuring chamber cover plate (101) with an opening, the side of the measuring chamber is provided with a photon through hole, the measuring chamber is internally provided with an accommodating cavity for accommodating a turntable (102), the turntable (102) is provided with a plurality of reaction cup accommodating positions (1021) which are arranged along the circumferential direction of the turntable (102) and are used for accommodating reaction cups, and the opening is used for placing the reaction cups into the reaction cup accommodating positions (1021) or taking the reaction cups away from the reaction cup accommodating positions (1021);

the driving unit (2) is used for driving the turntable (102) to rotate, so that the reaction cups successively flow among different stations of a reaction cup taking and placing position, a shaking position, a liquid injection or luminescence measuring position and a liquid absorption position;

a door opening and closing unit (3) for opening or closing an opening in the cover plate (101) of the measuring chamber;

the shaking unit (5) is used for carrying out shaking action on the reaction cups which are transferred to the shaking position;

the luminescence measurement unit (4) can close or open the photon via hole on the measurement chamber (1) and collect the luminescence quantity of the substance in the reaction cup on the injection or luminescence measurement position when the photon via hole is opened;

the liquid injection and absorption unit (6) is used for injecting liquid into the reaction cup corresponding to the liquid injection position and extracting waste liquid from the reaction cup corresponding to the liquid absorption position, and comprises a bracket (601), a driving component (602) and a needle component (603) which are fixed on a cover plate (101) of the measuring chamber; the needle assembly (603) comprises a liquid suction needle (6032), a liquid injection needle (6033) and a needle fixing plate (6031) fixedly connected with the liquid suction needle (6031) and the liquid injection needle (6033) and positioned at the upper part of the measuring chamber cover plate (101), the liquid suction needle (6032) and the liquid injection needle (6033) respectively penetrate through the measuring chamber cover plate (101) and correspond to reaction cups on a liquid suction level and a liquid injection level, and the needle point of the liquid suction needle (6032) and the needle point of the liquid injection needle (6033) have a certain height difference; the driving component (602) comprises a screw rod motor (6021), a transmission screw rod (6022), a screw rod nut (6034) which is screwed on the transmission screw rod (6022) and is fixedly connected with a needle fixing plate (6031), a driving seat (6023) which is fixed on the needle fixing plate (6031), a linear guide rail (6027) which is arranged on the bracket (601) and is parallel to the transmission screw rod (6022) and a sliding block (6026) which is fixed on the driving seat (6023) and is matched with the linear guide rail (6027), one end of the transmission screw rod (6022) is connected with an output shaft of the screw rod motor (6021), the other end is connected with the measuring chamber cover plate (101) through a bearing, the liquid suction needle (6032) and the liquid injection needle (6033) fixed on the needle fixing plate (6031) can move up and down through the screw rod motor (6021) and the transmission screw rod (6022), and the slide block (6026) is driven by the needle fixing plate (6031) and the driving seat (6023) to move up and down along the linear guide rail (6027).

2. The multi-cuvette continuous measurement luminescence measurement apparatus according to claim 1, wherein the liquid injection and aspiration unit (6) further comprises a liquid injection needle sealing assembly (604) and a liquid aspiration needle sealing assembly (605), the liquid injection needle sealing assembly (604) comprising: the liquid injection device comprises a sealing column (6043) arranged on a measuring chamber cover plate (101), a sealing column cover plate (6044) arranged at the top of the sealing column (6043), a first sealing plug (6042) arranged in a groove in the top of the sealing column (6043) and connected with the sealing column cover plate (6044), and a first O-shaped ring (6041) arranged on the periphery of the first sealing plug (6042), wherein a through hole for a liquid injection needle (6033) to pass through is formed in the sealing column cover plate (6044), the first sealing plug (6042) and the sealing column (6043), and a containing cavity with the diameter larger than the outer diameter of the liquid injection needle is formed at the bottom of the sealing column (6043); the pipette needle seal assembly (5) comprises: the second sealing plug (6052) is arranged in a groove in the top of the measuring chamber cover plate (101) and connected with a sealing plug cover plate (6051) arranged on the measuring chamber cover plate (101), and a second O-shaped ring (6053) is arranged on the periphery of the second sealing plug (6052), and through holes for liquid suction needles (6032) to pass through are formed in the sealing plug cover plate (6051) and the second sealing plug (6052); preferably, annotate between liquid needle (6033) and first sealing plug (6042), between imbibition needle (6032) and second sealing plug (6052), between first O shape circle (6041) and first sealing plug (6042), all adopt interference fit between second O shape circle (6053) and second sealing plug (6052), first sealing plug (6042), second sealing plug (6052) hole preferably adopts the bell mouth structure, first sealing plug (6042), second sealing plug (6052) preferred adopt wear-resisting black plastics.

3. The multi-cuvette continuous measurement light-emitting measurement apparatus according to claim 1 or 2, wherein the holder (601) is composed of a top plate (6011), a vertical plate (6012), and a base (6013), the base (6013) is fixed on the measurement chamber cover plate (101), and the linear guide rail (6027) is disposed on the vertical plate (6027); the upper end and the lower end of the vertical plate (6012) are preferably provided with two limit pins (6028) for limiting the slide block (6026).

4. The multi-reaction cup continuous measurement luminescence measurement apparatus according to any one of claims 1 to 3, wherein the driving seat (6023) is provided with a light coupling baffle plate (6024), and the riser (6012) is provided with a light coupling (6025) corresponding to the light coupling baffle plate (6024); before the turntable (102) rotates, a signal is sent to a control system through the induction of the optical coupler baffle plate (6024) and the optical coupler (6025), so that the resetting of the whole needle assembly (603) is realized; preferably, the liquid suction needle (6032) is connected with a waste liquid pipe, the waste liquid pipe is connected with a waste liquid pump, the liquid injection needle (6033) is connected with a liquid injection pipe, and the liquid injection pipe is connected with a liquid injection pump.

5. The multi-cuvette continuous measurement luminescence measurement apparatus according to any one of claims 1 to 4, wherein the driving unit (2) comprises a rotation power mechanism and a rotation transmission mechanism, the rotation transmission mechanism comprises a rotation shaft (211), and a driving synchronous pulley (204) and a driven synchronous pulley (206) connected through a synchronous belt (205), one end of the rotation shaft (211) is fixedly connected with the turntable (102) through a bearing (210), the other end is connected with the driven synchronous pulley (206) through a bearing (210) and a shaft collar (214), and the driving synchronous pulley (204) is fixedly connected with the rotation power mechanism; preferably, drive unit (2) still include canceling release mechanical system, canceling release mechanical system is including the opto-coupler (208) that resets and install in driven synchronous pulley (206) bottom with the opto-coupler (208) that resets corresponding opto-coupler separation blade (209) that resets, reset opto-coupler (208) through resetting opto-coupler support (207) be fixed in the measuring room on, before the official test, through the response that resets opto-coupler (208) and reset opto-coupler separation blade (209), thereby to control system signal transmission, make each reaction cup hold position (1021) and fix a position initial position.

6. The multi-cuvette continuous measurement luminescence measurement device according to any one of claims 1 to 5, wherein the door opening and closing unit (3) comprises a base body (301), and a door (302) and a door driving motor (303) and a transmission screw rod (304) mounted on the base body (301), one end of the transmission screw rod (304) is connected to an output shaft of the driving motor (303), the other end is connected to the door (302), a door opening is formed on the base body (301), the door opening corresponds to an opening of the measurement chamber cover plate (101), guide rails are arranged on two sides of the base body (301), and the operation of the door driving motor (303) can drive the door (302) to slide above the door opening along the guide rails.

7. The multi-reaction-cup continuous-measurement luminescence measurement device according to any one of claims 1 to 6, wherein the luminescence measurement unit (4) comprises a photomultiplier (401), a rotary driving member (404) mounted on the mounting plate (405), and a baffle plate (402) having a light-passing hole (4022), a sliding slot (4021) is further formed on the baffle plate (402), a rotary rocker (4031) positioned on the cam (403) and driven to rotate by the rotary driving member (404) is disposed in the sliding slot (4021), and when the rotary rocker (4031) rotates, the baffle plate (402) is horizontally moved by being matched with the sliding slot (4021), so that the through hole (4022) on the baffle plate (402) is overlapped or misaligned with the photon passing hole; preferably, the rotary driving member (404) comprises a rotary driving motor (4041) and a driving shaft (4042), one end of the driving shaft (4042) is connected with an output shaft of the driving motor (4041), the other end of the driving shaft is connected with a cam (403), a door opening positioning optical coupler (407) and a door closing positioning optical coupler (408) are arranged on two sides of the driving shaft (4042), and a positioning optical coupler blocking piece (406) which corresponds to the door opening positioning optical coupler (407) and the door closing positioning optical coupler (408) and is provided with an opening is fixedly arranged on the driving shaft (412), the door opening positioning optical coupler (407), the door closing positioning optical coupler (408) and the positioning optical coupler blocking piece (406) are used for sensing whether a photon through hole is blocked by a blocking plate (402) or not, and a signal is sent to a.

8. The multi-reaction cup continuous measurement light-emitting measuring device according to any one of claims 1 to 7, wherein the shaking unit (5) comprises an up-down displacement component and a shaking component, the up-down displacement component is used for controlling the lifting of the reaction cup, and when the up-down displacement component drives the reaction cup to lift, the shaking component can drive the reaction cup to eccentrically rotate; preferably, the up-down displacement assembly comprises a lifting power mechanism (501), a horizontally arranged moving support plate (502), a vertically arranged limiting plate (503) and a linear guide rail arranged on the limiting plate (503), wherein the lifting power mechanism (501) drives the moving support plate (502) to move up and down along the linear guide rail; preferably, the shaking component comprises a shaking power mechanism (504) and an extension rod (505), the shaking power mechanism (502) is connected with the movable support plate (502), one end of the extension rod (505) is fixedly connected with the shaking power mechanism (502), the other end of the extension rod is fixedly connected with an eccentric block (506), the eccentric block (506) can slide upwards along with the movable mechanism (502) to penetrate into the reaction cup containing position (1021), and the reaction cup positioned in the reaction cup containing position (1021) is jacked to abut against a reaction cup limiting mechanism positioned above the shaking position, so that the reaction cup is clamped and fixed by the reaction cup limiting mechanism positioned above and the eccentric block (506) positioned below.

9. A multi-cuvette continuous measurement luminescence measurement method, which is performed by using the multi-cuvette continuous measurement luminescence measurement apparatus according to any one of embodiments 1 to 8, comprising the steps of:

s1: starting the door opening and closing unit (3) to open the opening of the cover plate (101) of the measuring chamber (1), executing reaction cup taking action on the reaction cup taking and placing position, placing the reaction cup filled with the buffer solution 1 into the reaction cup containing position (1021) corresponding to the reaction cup taking and placing position, and then closing the opening of the cover plate (101) of the measuring chamber (1) through the door opening and closing unit (3);

s2: the rotating disc (102) is driven to rotate by the driving unit (2), so that reaction cups in the reaction cup containing position (1021) sequentially flow among different stations of a reaction cup taking and placing position, a shaking position, a liquid injection level and a liquid absorption position, the reaction cups which are rotated to the shaking position are shaken to be uniformly moved by the shaking unit (4), the reaction cups which are rotated to the liquid injection position are injected with liquid through the injection buffer liquid 2 and the liquid absorption unit (6) and are opened through the light-emitting measurement unit (4) so as to collect the light emission quantity of substances in the reaction cups, the reaction cups which are rotated to the liquid absorption position are subjected to liquid absorption movement on waste liquid in the reaction cups through the injection and liquid absorption unit (6), the reaction cup taking and removing movement are performed on the reaction cups which are rotated to the reaction cup taking and placing position, and new reaction cups are placed on the reaction cup taking and placing position; the liquid injection or luminescence measurement action or the placing or taking-away action of the reaction cup can be simultaneously carried out with the shaking and liquid absorption actions of the reaction cup respectively, the liquid injection or luminescence measurement action and the placing or taking-away action of the reaction cup can not be simultaneously carried out, the opening of the cover plate (101) of the measurement chamber (1) is closed when the liquid injection or luminescence measurement is carried out, and the photon through hole on the measurement chamber (1) is closed through the luminescence measurement unit (4) when the placing or taking-away action of the reaction cup is carried out.

10. The method for measuring luminescence of a multi-cuvette continuous measurement according to claim 9, wherein the luminescence measuring unit (4) is activated before the liquid injection by the liquid injection and absorption unit (6); preferably, during liquid injection and liquid suction, the liquid suction needle (6032) and the liquid injection needle (6033) are driven by the screw rod motor (6021) to slowly descend, liquid suction is always performed during descending until the liquid suction needle (6032) touches the bottom of the reaction cup, liquid injection is started when the needle point of the liquid injection needle (6033) enters the reaction cup (7), and the liquid injection needle (6033) cannot touch the buffer liquid in the reaction cup when the liquid suction needle (6032) touches the bottom of the reaction cup.

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