CN112191288B - Unicellular separator based on unicellular sequencing technology - Google Patents

Abstract

The invention discloses a single cell separation device based on a single cell sequencing technology, which comprises a machine body, wherein a separation cavity is arranged in the machine body, a confluence base is fixedly arranged between the left wall and the right wall of the lower side of the separation cavity, a microfluidic column is fixedly arranged on the upper end surface of the confluence base, microfluidic channel pipes are fixedly arranged in a circumferential array on the outer circumferential surface of the microfluidic column, a dripping inlet with an upward opening is arranged at the position of the center of a circle in the microfluidic column, each microfluidic channel pipe is internally communicated with a microfluidic channel with an outward opening, and an annular confluence groove with an upward opening is formed in the upper end surface of the confluence base and is connected with the outer side of the microfluidic channel pipe; the invention can realize the separation of single cells by using the micro-channel plate, and the micro-channel plate is deformed by applying micro pressure, thereby controlling the flowing tendency of the internal liquid of the micro-channel, being beneficial to the flowing of cell micro-flow liquid, and simultaneously being capable of automatically adding a fluorescent labeling reagent in the process of dripping the cell reagent, and being convenient for identifying cells.

Description

Unicellular separator based on unicellular sequencing technology

Technical Field

The invention relates to the technical field of single cell sequencing, in particular to a single cell separation device based on a single cell sequencing technology.

Background

Single cell transcriptome sequencing refers to a new technique for high throughput sequencing and analysis of RNA at the single cell level. Different from the results (only the average expression level of a large number of cells) obtained by sequencing conventional tissues or cell groups, single sequencing can deeply excavate specific information, the most important step of single cell sequencing is the separation of single cells, the single cell separation technology comprises the steps of micromanipulation separation, laser microdissection, flow cytometry, microfluidic technology and the like, the microfluidic technology is a cell separation technology with greater advantages, and the separation of the single cells can be realized by utilizing gravity centrifugation, hydrodynamics, electric field force and the like.

Disclosure of Invention

The invention aims to solve the technical problem of providing a single cell separation device based on a single cell sequencing technology, and solves the problems of liquid flow direction guide and inconvenience in adding a fluorescence labeling reagent of the microfluidic single cell separation device.

The invention is realized by the following technical scheme.

The invention relates to a single cell separation device based on a single cell sequencing technology, which comprises a machine body, wherein a separation cavity is arranged in the machine body, a confluence base is fixedly arranged between the left wall and the right wall of the lower side of the separation cavity, a microfluidic column is fixedly arranged on the upper end surface of the confluence base, microfluidic channel pipes are fixedly arranged on the circumferential array of the outer circumferential surface of the microfluidic column, a dripping port with an upward opening is arranged at the position of the center of a circle in the microfluidic column, a microfluidic channel with an outward opening is communicated in each microfluidic channel pipe, an annular confluence groove with an upward opening is arranged at the joint of the upper end surface of the confluence base and the outer side of the microfluidic channel pipe, a liquid outlet pipeline with a downward opening is arranged in the confluence base and under the dripping port, a confluence channel is communicated between the lower side of the confluence base and the circumferential wall of the upper side of the liquid outlet pipeline, and a control valve is fixedly arranged on the lower end surface of the confluence base and under the liquid outlet pipeline, thereby the control valve can control the whereabouts of the switching control liquid of drain pipe downside, thereby the organism is internal and communicate the separating chamber downside is equipped with about open-ended reagent board chamber, it is equipped with the reagent board to place on the reagent board chamber lower wall, evenly distributed is equipped with the ascending liquid groove of connecing of opening in the reagent board up end about, the fixed spliced pole that is equipped with of separating chamber upper wall, the fixed pressure disk that is equipped with of terminal surface under the spliced pole, terminal surface circumference array is equipped with the pressure column chamber that the opening is decurrent under the pressure disk, the gliding pressure column that is equipped with from top to bottom in the pressure column chamber, terminal surface offsets with miniflow pipe up end under the pressure column, make miniflow pipe produce the deformation that removes through pressure when the pressure column moves down to control the liquid flow in the equidirectional miniflow way, be equipped with the lift chamber in the spliced pole, the gliding lifter plate that is equipped with from top to bottom in the lift chamber, the fixed drip needle that is equipped with that runs through from top to bottom in the lifter plate, the fixed mixing block that is equipped with in drip needle middle part, be equipped with in the mixing block and switch the rotation chamber, it is equipped with mixed rotary drum to switch the rotation intracavity, it is equipped with the ascending connector of opening to mix rotary drum upside intercommunication, in the drip needle and mix a upside intercommunication switch the rotation chamber and be equipped with the ascending feed liquor passageway of opening, in the drip needle and be in mix a downside intercommunication switch the rotation chamber and be equipped with the decurrent drain passage of opening, intercommunication in the mixing block switches the upper right side of rotation chamber and is equipped with the interpolation passageway that the opening was right, in the spliced pole and go up and down the chamber right side with be equipped with the fluorescence reagent chamber between the rotary drum chamber, fluorescence reagent chamber downside left wall with the intercommunication is equipped with the connecting pipe between the interpolation passageway right side.

Preferably, just be in the organism reagent board chamber downside intercommunication is equipped with the translation chamber, the translation chamber is equipped with interior energy horizontal slip's slurcam spring, it accomodates the chamber to be equipped with the ascending slurcam of opening in the slurcam spring, the slurcam is accomodate the intracavity and is equipped with gliding slurcam from top to bottom, the slurcam can extend to reagent board intracavity and with reagent board left end face offsets, under the slurcam terminal surface with the slurcam accomodates fixedly connected with slurcam spring between the chamber lower wall, the slurcam under the terminal surface with the slurcam accomodates the chamber lower wall and fixes the electro-magnet that is equipped with respectively and can inhale mutually by opposite polarities, it is equipped with the translation screw rod to rotate between the wall about the translation chamber.

Preferably, a translation motor is fixedly arranged in the right wall of the translation cavity, and the right end of the translation screw is in power connection with the left end face of the translation motor.

Preferably, in the pressure dish and communicate all jointly pressure column chamber upside is equipped with the rotation chamber, be equipped with annular rotary drum chamber in the spliced pole, the rotary drum intracavity rotates and is equipped with the rotary drum, the rotary drum lower extreme extends to rotate intracavity and outer disc fixed pressure switching ring that is equipped with, pressure column upper end extends to rotate intracavity and the fixed board that resets of up end, reset under the board terminal surface with rotate between the chamber lower wall and around in pressure column outside fixedly connected with reset spring, the fixed lower briquetting that is equipped with down the terminal surface ability with the up end of reset plate offsets under the pressure switching ring left side terminal surface.

Preferably, the inner circular wall at the left side of the rotary cylinder cavity is communicated with a rotary gear cavity, a rotary driving shaft is rotatably arranged between the upper wall and the lower wall of the rotary gear cavity, a rotating gear meshed with the inner circular surface on the upper side of the rotary drum is fixedly arranged on the outer circular surface of the rotating driving shaft, a driving device is fixedly arranged in the upper wall of the rotating gear cavity, the upper end of the rotating driving shaft is in power connection with the lower end surface of the driving device, a lifting screw rod is rotatably arranged on the upper end surface of the pressure disc and on the left side of the dropping needle, a lifting driven gear is fixedly arranged on the outer circular surface of the upper end of the lifting screw rod, a lifting gear cavity with a right opening is arranged in the connecting column and at the upper side of the rotating gear cavity, a lifting driving shaft is rotatably arranged between the upper wall and the lower wall of the lifting gear cavity, and a lifting driving gear meshed with the lifting driven gear is fixedly arranged on the outer circular surface of the lifting driving shaft, and the lower end of the lifting driving shaft is connected with the upper end surface of the driving device through a cable.

Preferably, the outer round surface of the mixing drum is internally provided with arc-shaped rotating teeth fixedly arranged at the rear side of the mixing cavity, the left side of the switching rotating cavity is communicated with a driving gear cavity, a driving gear shaft is rotatably arranged between the front wall and the rear wall of the driving gear cavity, a driving gear meshed with the rotating teeth is fixedly arranged on the outer round surface of the driving gear shaft, a micro motor is fixedly arranged in the rear wall of the driving gear cavity, and the rear end of the driving gear shaft is in power connection with the front end face of the micro motor.

The invention has the beneficial effects that: the invention can realize the separation of single cells by using the micro-channel plate, and the micro-channel tube deforms by applying micro pressure, thereby controlling the flowing tendency of the internal liquid of the micro-channel, being beneficial to the flowing of cell micro-flow liquid, and simultaneously being capable of automatically adding a fluorescent labeling reagent in the process of dripping the cell reagent, and being convenient for identifying cells.

Drawings

In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an embodiment of the present invention;

FIG. 2 is an enlarged schematic view of the embodiment of the present invention at A in FIG. 1;

FIG. 3 is a schematic structural diagram of B-B in FIG. 2 according to an embodiment of the present invention;

FIG. 4 is an enlarged schematic view of the embodiment of the present invention at C in FIG. 1;

FIG. 5 is an enlarged schematic view of the embodiment of the present invention at D in FIG. 1;

FIG. 6 is an enlarged schematic view of the embodiment of the present invention at E in FIG. 1.

Detailed Description

The invention will now be described in detail with reference to fig. 1-6, wherein for ease of description the orientations described hereinafter are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.

The unicell separation device based on the unicell sequencing technology, which is described in conjunction with the accompanying drawings 1-6, includes amachine body 11, aseparation chamber 12 is arranged in themachine body 11, aconfluence base 14 is fixedly arranged between the left and right walls of the lower side of theseparation chamber 12, amicrofluidic column 76 is fixedly arranged on the upper end surface of theconfluence base 14,microfluidic channel pipes 31 are fixedly arranged on the circumferential array of the outer circumferential surface of themicrofluidic column 76, adripping port 33 with an upward opening is arranged at the inner center of themicrofluidic column 76, amicrofluidic channel 32 with an outward opening is communicated in eachmicrofluidic channel pipe 31, anannular confluence groove 30 with an upward opening is arranged at the joint of the upper end surface of theconfluence base 14 and the outer side of themicrofluidic channel pipe 31, aliquid outlet pipe 28 with a downward opening is arranged in theconfluence base 14 and under thedripping port 33, aconfluence channel 29 is communicated between the lower side of theconfluence groove 30 and the circumferential wall of the upper side of theliquid outlet pipe 28, the lower end face of theconfluence base 14 is fixedly provided with acontrol valve 24 under theliquid outlet pipeline 28, thecontrol valve 24 can control the opening and closing of the lower side of theliquid outlet pipeline 28 to control the falling of liquid, themachine body 11 is communicated with areagent plate cavity 13 with a left opening and a right opening, which is arranged at the lower side of theseparation cavity 12, areagent plate 23 is arranged on the lower wall of thereagent plate cavity 13, liquid receivinggrooves 25 with upward openings are uniformly distributed in the upper end face of thereagent plate 23 from left to right, a connectingcolumn 15 is fixedly arranged on the upper wall of theseparation cavity 12, apressure disc 16 is fixedly arranged on the lower end face of the connectingcolumn 15, apressure column cavity 71 with a downward opening is arranged on the circumferential array of the lower end face of thepressure disc 16, apressure column 54 capable of sliding up and down is arranged in thepressure column cavity 71, the lower end face of thepressure column 54 is abutted against the upper end face of themicro-flow channel pipe 31, and themicro-channel pipe 31 is deformed by pressure when thepressure column 54 moves downwards, thereby controlling the liquid flow in theequidirectional microchannel 32 of equidirectional, be equipped withlift chamber 70 in thespliced pole 15, thegliding lifter plate 35 that is equipped with about in thelift chamber 70, be equipped with the fixeddrip needle 36 that runs through from top to bottom in thelifter plate 35,drip needle 36 middle part is fixed and is equipped withmixing block 39, be equipped with in themixing block 39 and switch and rotatechamber 41, switch and rotate theintracavity 41 internal rotation and be equipped with mixingdrum 42, mixingdrum 42 upside intercommunication is equipped with the ascending connector 44 of opening, in thedrip needle 36 and be inmixing block 39 upside intercommunication switch androtate chamber 41 and be equipped with the ascending inlet channel 37 of opening, in thedrip needle 36 and be in mixingblock 39 downside intercommunication switch androtate chamber 41 and be equipped with thedecurrent outlet channel 38 of opening, the intercommunication in themixing block 39 switching is rotated the upper right side ofchamber 41 and is equipped with theright interpolation passageway 50 of opening, afluorescent reagent cavity 19 is arranged between the right side of thelifting cavity 70 and therotary drum cavity 20 in the connectingcolumn 15, and a connectingpipe 40 is communicated between the left wall of the lower side of thefluorescent reagent cavity 19 and the right side of the addingchannel 50.

Beneficially,translation chamber 22 is arranged in themachine body 11 and communicated with the lower side of thereagent plate chamber 13, a pushingplate spring 73 capable of sliding left and right is arranged in thetranslation chamber 22, a pushingplate accommodating chamber 75 with an upward opening is arranged in the pushingplate spring 73, a pushingplate 57 capable of sliding up and down is arranged in the pushingplate accommodating chamber 75, the pushingplate 57 can extend into thereagent plate chamber 13 and abut against the left end face of thereagent plate 23, a pushingplate spring 73 is fixedly connected between the lower end face of the pushingplate 57 and the lower wall of the pushingplate accommodating chamber 75,electromagnets 56 capable of absorbing in opposite directions are fixedly arranged on the lower end face of the pushingplate 57 and the lower wall of the pushingplate accommodating chamber 75 respectively, andtranslation screw rods 26 are arranged between the left wall and the right wall of thetranslation chamber 22 in a rotating manner.

Advantageously, a translation motor 27 is fixedly arranged in the right wall of thetranslation cavity 22, and the right end of thetranslation screw 26 is in power connection with the left end face of the translation motor 27.

Beneficially, a rotatingcavity 17 is formed in thepressure disc 16 and is communicated with the upper sides of all thepressure column cavities 71 together, anannular drum cavity 20 is formed in the connectingcolumn 15, adrum 21 is rotatably formed in thedrum cavity 20, the lower end of thedrum 21 extends into therotating cavity 17, apressure switching ring 18 is fixedly arranged on the outer circular surface of the drum, the upper end of thepressure column 54 extends into therotating cavity 17, areset plate 53 is fixedly arranged on the upper end surface of thepressure column 54, areset spring 55 is fixedly connected between the lower end surface of thereset plate 53 and the lower wall of the rotatingcavity 17 and surrounds the outer side of thepressure column 54, and alower press block 52, the lower end surface of which can abut against the upper end surface of thereset plate 53, is fixedly arranged on the lower end surface of the left side of thepressure switching ring 18.

Advantageously, a rotating gear chamber 64 is communicated with the inner wall of the left side of thedrum chamber 20, a rotating driving shaft 72 is rotatably arranged between the upper wall and the lower wall of the rotating gear chamber 64, arotating gear 66 engaged with the inner wall of the upper side of thedrum 21 is fixedly arranged on the outer circumferential surface of the rotating driving shaft 72, adriving device 63 is fixedly arranged on the upper wall of the rotating gear chamber 64, the upper end of the rotating driving shaft 72 is connected with the lower end surface of thedriving device 63 in a power manner, alifting screw 62 is rotatably arranged on the upper end surface of thepressure plate 16 and on the left side of thedrip needle 36, a lifting drivengear 61 is fixedly arranged on the outer circumferential surface of the upper end of thelifting screw 62, alifting gear chamber 58 with a right opening is arranged in the connectingcolumn 15 and on the upper side of the rotating gear chamber 64, a liftingdriving shaft 59 is rotatably arranged between the upper wall and the lower wall of thelifting gear chamber 58, a liftingdriving gear 60 engaged with the lifting drivengear 61 is fixedly arranged on the outer circumferential surface of thelifting driving shaft 59, the lower end of the liftingdriving shaft 59 is connected with the upper end surface of thedriving device 63 through a cable.

Beneficially, an arc-shaped rotating tooth 45 is fixedly arranged in the outer circumferential surface of themixing drum 42 and at the rear side of themixing chamber 43, adriving gear chamber 47 is communicated with the left side of the switching rotatingchamber 41, adriving gear shaft 49 is rotatably arranged between the front wall and the rear wall of thedriving gear chamber 47, adriving gear 48 meshed with the rotating tooth 45 is fixedly arranged on the outer circumferential surface of thedriving gear shaft 49, amicro motor 51 is fixedly arranged in the rear wall of thedriving gear chamber 47, and the rear end of thedriving gear shaft 49 is dynamically connected to the front end surface of themicro motor 51.

In the initial state, thelifting plate 35 is located at the upper limit position, the droppingneedle 36 is located at the upper limit position, all thepressure columns 54 are located at the upper limit position, the connecting port 44 rotates to be communicated with the liquid inlet channel 37, the pushingplate 57 is located at the upper limit position, the right end face of the pushing plate is abutted against the left end face of thereagent plate 23, and thereset plate 53 is located at the left limit position.

When the device works, a reagent with cells flows into themixing cavity 43 through the liquid inlet channel 37 and the connecting port 44, then thedriving device 63 is started, thelifting driving shaft 59 is driven to rotate and the liftingdriving gear 60 is driven to rotate through power connection, the lifting drivengear 61 is driven to rotate and thelifting screw 62 is driven to rotate through gear meshing, thelifting plate 35 is driven to move downwards through threaded connection, the droppingneedle 36 is driven to move downwards until the lower end extends into the droppingopening 33, then themicro motor 51 is started, thedriving gear shaft 49 is driven to rotate and thedriving gear 48 is driven to rotate through power connection, the rotating teeth 45 is driven to rotate and themixing drum 42 is driven to rotate through gear meshing, then the connecting port 44 is rotated to be communicated with the addingchannel 50, and then a fluorescence labeling reagent in the fluorescencelabeling reagent cavity 19 enters themixing cavity 43 through the connectingpipe 40 and the addingchannel 50 to be mixed with the cell reagent, then themixing drum 42 continues to rotate to connect the connection port 44 with theliquid outlet channel 38, and then the mixed reagent in themixing chamber 43 flows out downwards through the connection port 44 and theliquid outlet channel 38 to thedrop port 33, at this time, thedriving device 63 drives the rotating driving shaft 72 to rotate through power connection and drives therotating gear 66 to rotate, further drives thedrum 21 to rotate through gear engagement, further drives thepressure switching ring 18 to rotate and drives the lower pressingblock 52 to rotate, thereby rotating the lowerpressing block 52 to the upper end surface of one of the returningplates 53, further pressing the returningplate 53 downwards and compressing the returningspring 55, further driving thepressure column 54 to move downwards, further causing the microflow channel tube 31 to deform and control and guide the micro fluid in the correspondingmicro flow channel 32 to move away from thedrop port 33, thereby flowing into theconfluence groove 30 and flowing into theliquid outlet channel 28 through theconfluence channel 29, the liquid in theliquid outlet pipeline 28 is controlled to flow out through thecontrol valve 24, the translation motor 27 is started, thetranslation screw rod 26 is driven through power connection, thetranslation slide block 74 is driven to move rightwards through threaded connection, thepush plate 57 is driven to move rightwards and thereagent plate 23 is driven to move rightwards, each liquid receivinggroove 25 is filled with cell unicellular liquid, thereagent plate 23 is driven to move rightwards to the right side of themachine body 11 to be taken out conveniently, then the translation motor 27 is started reversely, thepush plate 57 is driven to move leftwards through corresponding transmission to reset, theelectromagnet 56 is electrified, thepush plate 57 is driven to move downwards through magnetic force opposite attraction and pushes theplate spring 73, and then thenext reagent plate 23 is placed onto the lower wall of thereagent plate cavity 13 from the left side of thereagent plate cavity 13 to be taken out for the next reagent plate.

The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (5)

1. A unicellular separator based on unicellular sequencing technology, includes the organism, its characterized in that: a separation cavity is arranged in the machine body, a confluence base is fixedly arranged between the left wall and the right wall of the lower side of the separation cavity, a micro flow column is fixedly arranged on the upper end face of the confluence base, a micro flow channel pipe is fixedly arranged on the circumferential array of the outer circumferential surface of the micro flow column, a dripping port with an upward opening is arranged at the position of the center of a circle in the micro flow column, a micro flow channel with an outward opening is arranged in each micro flow channel pipe in a communicated manner, an annular confluence groove with an upward opening is arranged at the joint of the upper end face of the confluence base and the outer side of the micro flow channel pipe, a liquid outlet pipeline with a downward opening is arranged in the confluence base and under the dripping port, a confluence channel is arranged between the lower side of the confluence groove and the upper side of the circumferential wall of the liquid outlet pipeline in a communicated manner, a control valve is fixedly arranged on the lower end face of the confluence base and under the liquid outlet pipeline, and can control the opening and closing of the lower side of the liquid outlet pipeline so as to control the falling of liquid, open-ended reagent board chamber about just communicateing the separation chamber downside is equipped with in the organism, it is equipped with the reagent board to place on the reagent board chamber lower wall, evenly distributed is equipped with the ascending cistern that connects of opening about in the reagent board up end, the fixed spliced pole that is equipped with of separation chamber upper wall, the fixed pressure disk that is equipped with of terminal surface under the spliced pole, terminal surface circumference array is equipped with the pressure column chamber that the opening is decurrent under the pressure disk, be equipped with gliding pressure column from top to bottom in the pressure column chamber, terminal surface offsets with microchannel pipe up end under the pressure column, make microchannel pipe produce the deformation that removes through pressure when the pressure column moves down to the liquid flow in the control equidirectional microchannel, be equipped with the lift chamber in the spliced pole, gliding lifter plate is equipped with from top to bottom in the lift chamber, the lifter plate is equipped with the fixed drip needle that runs through from top to bottom in, the mixing block is fixedly arranged in the middle of the liquid dropping needle, a switching rotating cavity is arranged in the mixing block, a mixing rotary drum is rotatably arranged in the switching rotating cavity, a connecting port with an upward opening is formed in the upper side of the mixing rotary drum in a communicated manner, a liquid inlet channel with an upward opening is formed in the mixing block in a communicated manner, a liquid outlet channel with a downward opening is formed in the switching rotating cavity in the liquid dropping needle and in a communicated manner at the lower side of the mixing block, an adding channel with a rightward opening is formed in the mixing block in a communicated manner at the upper right side of the switching rotating cavity, a rotating cavity is formed in the pressure disc and is communicated with all the upper sides of the pressure column cavities together, an annular rotary drum cavity is arranged in the connecting column, a rotary drum is rotatably arranged in the rotary drum cavity, the lower end of the rotary drum extends into the rotary cavity, a pressure switching ring is fixedly arranged on the outer circular surface of the rotary cavity, and a reset plate is fixedly arranged on the upper end surface of the rotary cavity, reset under the board terminal surface with rotate between the chamber lower wall and around in pressure column outside fixedly connected with reset spring, under the pressure switching ring left side terminal surface fixed be equipped with down the terminal surface can with the lower briquetting that resets the counterbalance of board up end, just in the spliced pole go up and down the chamber right side with be equipped with the fluorescence reagent chamber between the rotary drum chamber, fluorescence reagent chamber downside left wall with the intercommunication is equipped with the connecting pipe between the interpolation passageway right side.

2. A single-cell separation device based on single-cell sequencing technology as claimed in claim 1, characterized in that: the internal just is in reagent board chamber downside intercommunication is equipped with the translation chamber, the translation intracavity is equipped with the slurcam spring that can the horizontal slip, it accomodates the chamber to be equipped with the ascending slurcam of opening in the slurcam spring, the slurcam is accomodate the intracavity and is equipped with gliding slurcam from top to bottom, the slurcam can extend to reagent board intracavity and with reagent board left end face offsets, the slurcam lower terminal surface with the slurcam accomodates fixedly connected with slurcam spring between the chamber lower wall, the slurcam lower terminal surface with the slurcam accomodates the chamber lower wall and is fixed respectively to be equipped with the electro-magnet that can opposite sex looks inhale, it is equipped with the translation screw rod to rotate between the wall about the translation chamber.

3. A single-cell separation device based on single-cell sequencing technology as claimed in claim 2, characterized in that: translation chamber right side wall internal fixation is equipped with the translation motor, translation screw rod right-hand member power connect in translation motor left end face.

4. A single-cell separation device based on single-cell sequencing technology as claimed in claim 1, characterized in that: the inner circular wall at the left side of the rotary cylinder cavity is communicated with a rotary gear cavity, a rotary driving shaft is rotatably arranged between the upper wall and the lower wall of the rotary gear cavity, a rotating gear meshed with the inner circular surface on the upper side of the rotary drum is fixedly arranged on the outer circular surface of the rotating driving shaft, a driving device is fixedly arranged in the upper wall of the rotating gear cavity, the upper end of the rotating driving shaft is in power connection with the lower end surface of the driving device, a lifting screw rod is rotatably arranged on the upper end surface of the pressure disc and on the left side of the dropping needle, a lifting driven gear is fixedly arranged on the outer circular surface of the upper end of the lifting screw rod, a lifting gear cavity with a right opening is arranged in the connecting column and at the upper side of the rotating gear cavity, a lifting driving shaft is rotatably arranged between the upper wall and the lower wall of the lifting gear cavity, and a lifting driving gear meshed with the lifting driven gear is fixedly arranged on the outer circular surface of the lifting driving shaft, and the lower end of the lifting driving shaft is connected with the upper end surface of the driving device through a cable.

5. A single-cell separation device based on single-cell sequencing technology as claimed in claim 1, characterized in that: the utility model discloses a mixing drum, including mixing drum, switching rotation chamber, driving gear axle excircle, mixing drum excircle in the face and at the fixed curved rotation tooth that is equipped with in mixing chamber rear side, switching rotation chamber left side intercommunication is equipped with the driving gear chamber, it is equipped with driving gear axle to rotate between the front and back wall in driving gear chamber, driving gear axle excircle fixed be equipped with the driving gear of rotation tooth meshing, driving gear chamber back wall internal fixation is equipped with the micromotor, driving gear axle rear end power connect in terminal surface before the micromotor.

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