CN111925808A - Soil remediation agent based on high cadmium-resistant bacterial strain - Google Patents

Abstract

Translated fromChinese

本发明涉及土壤修复处理技术领域，具体是公开了一种基于高耐镉菌株的土壤修复剂，它是由以下重量份的原料制成：微生物菌剂和复合粉剂，所述微生物菌剂采用由污染土壤中分离出的高耐镉菌株QN‑3制成，所述复合粉剂其成分以重量份数计算含有：改性凹凸捧土50‑80份、硅藻土12‑20份、农作物秸秆20‑26份、家禽牲畜粪便15‑22份和糖渣5‑8份，所述高耐镉菌株QN‑3于2018年05月28日保藏于广东省微生物菌种保藏中心(GDMCC)，菌种保藏号为GDMCC No：60378。本发明克服了现有技术的不足，利用微生物与复合粉剂的配合可显著提高受污染土壤中有机质含量，调节土壤菌群结构及土壤酸碱性，提高土壤透气性，改善土壤理化性质，可以有效降解土壤中有机污染物及重金属的含量。The invention relates to the technical field of soil remediation treatment, and in particular discloses a soil remediation agent based on high cadmium-resistant strains. The high cadmium-tolerant strain QN-3 isolated from the polluted soil is prepared, and the composite powder contains in parts by weight: 50-80 parts of modified concave-convex clay, 12-20 parts of diatomite, and 20 parts of crop straw. -26 parts, 15-22 parts of poultry and livestock manure and 5-8 parts of sugar residue, the high cadmium-resistant strain QN-3 was deposited in the Guangdong Provincial Microorganism Culture Collection Center (GDMCC) on May 28, 2018. The deposit number is GDMCC No: 60378. The invention overcomes the deficiencies of the prior art, and can significantly increase the organic matter content in the polluted soil by using the combination of microorganisms and the composite powder, adjust the soil flora structure and soil acidity and alkalinity, improve soil permeability, and improve soil physical and chemical properties, and can effectively Degrade the content of organic pollutants and heavy metals in soil.

Description

Translated fromChinese

一种基于高耐镉菌株的土壤修复剂A soil remediation agent based on highly cadmium-tolerant strains

技术领域technical field

本发明涉及土壤修复处理技术领域，具体属于一种基于高耐镉菌株的土壤修复剂。The invention relates to the technical field of soil remediation treatment, in particular to a soil remediation agent based on high cadmium-tolerant strains.

背景技术Background technique

土壤是指陆地表面具有肥力、能够生长植物的疏松表层，其厚度一般在2m左右，土壤不但为植物生长提供机械支撑能力，并能为植物生长发育提供所需要的水、肥、气、热等肥力要素。近年来，由于人口急剧增长，工业迅猛发展，固体废物不断向土壤表面堆放和倾倒，有害废水不断向土壤中渗透，汽车排放的废气，大气中的有害气体及飘尘不断随雨水降落在土壤中。农业化学水平的提高，使大量化学肥料及农药散落到环境中，导致土壤遭受非点源污染的机会越来越多，其程度也越来越严重，在水土流失和风蚀作用等的影响下，污染面积不断扩大。当土壤中有害物质过多，超过土壤的自净能力，引起土壤的组成、结构和功能发生变化，微生物活动受到抑制，有害物质或其分解产物在土壤中逐渐积累，通过生物循环途径被人体吸收，达到危害人体健康的程度，就是土壤污染。Soil refers to the loose surface layer on the land surface that is fertile and capable of growing plants. Its thickness is generally about 2m. Fertility factor. In recent years, due to the rapid growth of population and the rapid development of industry, solid waste has been continuously stacked and dumped on the soil surface, harmful waste water has been continuously infiltrating into the soil, exhaust gas from automobiles, harmful gases and floating dust in the atmosphere have been continuously falling into the soil with rainwater. The improvement of the level of agricultural chemistry has caused a large number of chemical fertilizers and pesticides to be scattered into the environment, resulting in more and more opportunities for soil to suffer from non-point source pollution, and its degree has become more and more serious. Under the influence of soil erosion and wind erosion, The area of pollution continues to expand. When there are too many harmful substances in the soil, which exceed the self-purification capacity of the soil, the composition, structure and function of the soil are changed, the microbial activities are inhibited, and the harmful substances or their decomposition products gradually accumulate in the soil and are absorbed by the human body through the biological cycle. To the extent that it endangers human health, it is soil pollution.

重金属污染土壤的修复主要基于2种策略：一是去除化，将重金属从土壤中去除，达到清洁土壤的目的；二是固定化，将重金属固定在土壤中限制其释放，从而降低其风险。近年来，关于重金属污染土壤的修复技术研究取得了长足发展，按照工艺原理主要分为物理/化学修复、生物修复和农业生态修复。The remediation of heavy metal-contaminated soil is mainly based on two strategies: one is removal, which removes heavy metals from the soil to achieve the purpose of cleaning the soil; the other is immobilization, which fixes heavy metals in the soil to limit their release, thereby reducing their risks. In recent years, the research on the remediation technology of heavy metal-contaminated soil has made great progress. According to the process principle, it is mainly divided into physical/chemical remediation, bioremediation and agricultural ecological restoration.

微生物修复利用土著或者外源微生物群，在适宜的环境条件下，促进或强化微生物降解功能，从而达到降低有毒污染物活性或降解成无毒物质的生物修复技术。因此，开发一种低成本、简便、高效、环境友好的微生物土壤修复剂，在我国有着重要的意义和迫切性。Microbial remediation is a bioremediation technology that uses indigenous or exogenous microbial groups to promote or strengthen microbial degradation under suitable environmental conditions, thereby reducing the activity of toxic pollutants or degrading them into non-toxic substances. Therefore, the development of a low-cost, simple, efficient and environmentally friendly microbial soil remediation agent is of great significance and urgency in my country.

发明内容SUMMARY OF THE INVENTION

本发明的目的是提供了一种基于高耐镉菌株的土壤修复剂，克服了现有技术的不足，利用微生物与复合粉剂的配合可显著提高受污染土壤中有机质含量，调节土壤菌群结构及土壤酸碱性，提高土壤透气性，改善土壤理化性质，可以有效降解土壤中有机污染物及重金属的含量。The purpose of the present invention is to provide a soil remediation agent based on high cadmium-resistant strains, which overcomes the deficiencies of the prior art, and can significantly increase the organic matter content in the contaminated soil by using the combination of microorganisms and composite powders, adjust the soil flora structure and Soil acidity and alkalinity, improve soil permeability, improve soil physical and chemical properties, and can effectively degrade the content of organic pollutants and heavy metals in soil.

为解决上述问题，本发明所采取的技术方案如下：In order to solve the above-mentioned problems, the technical scheme adopted by the present invention is as follows:

一种基于高耐镉菌株的土壤修复剂，它是由以下重量份的原料制成：微生物菌剂和复合粉剂，所述微生物菌剂采用由污染土壤中分离出的高耐镉菌株QN-3制成，所述复合粉剂其成分以重量份数计算含有：改性凹凸捧土50-80份、硅藻土12-20份、农作物秸秆20-26份、家禽牲畜粪便15-22 份和糖渣5-8份。A soil remediation agent based on high cadmium-resistant strains, which is made from the following raw materials in parts by weight: microbial inoculum and composite powder, and the microbial inoculum adopts the high cadmium-resistant strain QN-3 isolated from polluted soil The composition of the composite powder, calculated in parts by weight, contains: 50-80 parts of modified attapulgite, 12-20 parts of diatomaceous earth, 20-26 parts of crop straw, 15-22 parts of poultry and livestock manure and sugar. 5-8 servings of slag.

进一步，所述高耐镉菌株QN-3于2018年05月28日保藏于广东省微生物菌种保藏中心(GDMCC)，菌种保藏号为GDMCC No：60378。Further, the high cadmium-resistant strain QN-3 was deposited in the Guangdong Provincial Microorganism Culture Collection Center (GDMCC) on May 28, 2018, and the culture collection number is GDMCC No: 60378.

进一步，所述改性凹凸捧土的制备方法：称取一定量的凹凸捧土，加入质量分数为0.1-0.2％的聚乙烯吡咯烷酮溶液和质量分数为0.2-0.3％的硼氢化钠溶液，搅拌均匀后在80-120℃下保温8-12分钟，取出冷却，然后加入白醋，混匀，最后放入冷冻干燥机干燥成固体，将固体粉碎成粉末，即得改性凹凸捧土。Further, the preparation method of the modified attapulgite: weighing a certain amount of attapulgite, adding a polyvinylpyrrolidone solution with a mass fraction of 0.1-0.2% and a sodium borohydride solution with a mass fraction of 0.2-0.3%, stirring After uniform, keep the temperature at 80-120°C for 8-12 minutes, take out and cool, then add white vinegar, mix well, and finally put it into a freeze dryer to dry into a solid, and pulverize the solid into powder to obtain modified attapulgite.

进一步，所述土壤修复剂的制备方法包括以下步骤：Further, the preparation method of the soil remediation agent comprises the following steps:

(1)微生物菌剂的制备：在无菌条件下，经过灭菌的容器中，高耐镉菌株QN-3按照液体培养基质量的4.5％接种到液体培养基中，于22-25℃下振荡培养3天，得到种子菌液，然后将种子菌液以9％的接种量接种到液体培养基中，于28-30℃下振荡培养3-5天得到发酵菌液，直接冷冻干燥得到微生物菌剂。(1) Preparation of microbial inoculum: Under sterile conditions, in a sterilized container, the high cadmium-resistant strain QN-3 was inoculated into the liquid medium according to 4.5% of the quality of the liquid medium, and the temperature was 22-25°C. Shake culture for 3 days to obtain seed bacteria liquid, then inoculate the seed bacteria liquid with 9% of the inoculum into the liquid medium, shake culture at 28-30 ° C for 3-5 days to obtain fermentation bacteria liquid, and directly freeze-dry to obtain microorganisms Bacterial agent.

(2)复合粉剂的制备：按照重量份数依次称取改性凹凸捧土、硅藻土、农作物秸秆、家禽牲畜粪便和糖渣，分别将上述原料置于粉碎机中粉碎，随后一起置于搅拌釜中搅拌均匀，放置3-5天后烘干粉碎过筛，即得复合粉剂。(2) Preparation of composite powder: Weigh modified bump clay, diatomite, crop straw, poultry and livestock manure and sugar residue in turn according to parts by weight, place the above-mentioned raw materials in a pulverizer and pulverize them together, and then place them together in a pulverizer. Stir evenly in the stirring tank, put it for 3-5 days, dry, pulverize and sieve to obtain composite powder.

进一步，所述家禽牲畜粪便为猪粪、牛粪、羊粪、鸡粪或鸭粪中的任意一种或者多种，所述农作物秸秆为小麦秸秆、棉花秸秆、玉米秸秆或大豆秸秆中的任意一种或者多种。Further, the poultry and livestock manure is any one or more of pig manure, cow manure, sheep manure, chicken manure or duck manure, and the crop straw is any one of wheat straw, cotton straw, corn straw or soybean straw. one or more.

进一步，所述土壤修复剂的使用方法为：在镉污染严重的土壤表面平铺一层复合粉剂，形成厚度为5-8mm的复合粉剂层，然后向复合粉剂层上均匀的喷洒微生物菌剂，翻耕混匀并补水，放置一段时间，期间定期洒水保持土壤的含水量并定期搅拌土壤。Further, the use method of the soil remediation agent is: laying a layer of composite powder on the soil surface with serious cadmium pollution, forming a composite powder layer with a thickness of 5-8mm, and then evenly spraying the microbial inoculum on the composite powder layer, Plough, mix well and replenish water, leave it for a period of time, sprinkle water regularly during the period to maintain the moisture content of the soil and stir the soil regularly.

进一步，1kg复合粉剂上喷洒的微生物菌剂的重量为100-200g。Further, the weight of the microbial inoculum sprayed on the 1kg composite powder is 100-200g.

本发明与现有技术相比较，本发明的实施效果如下：The present invention is compared with the prior art, and the implementation effect of the present invention is as follows:

本发明所述一种基于高耐镉菌株的土壤修复剂，利用微生物与复合粉剂的配合可显著提高受污染土壤中有机质含量，调节土壤菌群结构及土壤酸碱性，提高土壤透气性，改善土壤理化性质，可以有效降解土壤中有机污染物及重金属的含量；同时还具备利于养分保持，延长肥效，促进作物对养分的吸收，增加作物产量的优点，提高土壤肥力，作物增产增收效果显著；微生物采用自重金属污染土壤中分离出的高耐镉菌株，复合粉剂的各个组分均为常见的材料组分，降低了成本，具有重大的社会和经济价值。The soil remediation agent based on high cadmium-resistant strains of the invention can significantly increase the organic matter content in the polluted soil by using the combination of microorganisms and composite powders, adjust the soil flora structure and soil acidity and alkalinity, improve soil permeability, and improve soil permeability. The physical and chemical properties of the soil can effectively degrade the content of organic pollutants and heavy metals in the soil; at the same time, it also has the advantages of being conducive to nutrient retention, prolonging the fertilizer efficiency, promoting the absorption of nutrients by crops, increasing crop yield, improving soil fertility, and increasing crop yield and income. The microorganism adopts high cadmium-resistant strains isolated from heavy metal polluted soil, and each component of the composite powder is a common material component, which reduces the cost and has great social and economic value.

具体实施方式Detailed ways

下面结合实施例对本发明作进一步的描述，但本发明不仅限于这些实例，在为脱离本发明宗旨的前提下，所为任何改进均落在本发明的保护范围之内。Below in conjunction with embodiment, the present invention is further described, but the present invention is not limited to these examples, and under the premise of departing from the purpose of the present invention, any improvement for all falls within the protection scope of the present invention.

一种基于高耐镉菌株的土壤修复剂：A soil remediation agent based on highly cadmium-tolerant strains:

它是由以下重量份的原料制成：微生物菌剂和复合粉剂，所述微生物菌剂采用由污染土壤中分离出的高耐镉菌株QN-3制成，所述复合粉剂其成分以重量份数计算含有：改性凹凸捧土50-80份、硅藻土12-20份、农作物秸秆20-26份、家禽牲畜粪便15-22份和糖渣5-8份。It is made from the following raw materials in parts by weight: a microbial inoculum and a composite powder, the microbial inoculum is made from a high cadmium-resistant strain QN-3 isolated from polluted soil, and the composite powder is in parts by weight. The calculation contains: 50-80 parts of modified bump clay, 12-20 parts of diatomaceous earth, 20-26 parts of crop straw, 15-22 parts of poultry and livestock manure and 5-8 parts of sugar residue.

所述高耐镉菌株QN-3于2018年05月28日保藏于广东省微生物菌种保藏中心(GDMCC)，菌种保藏号为GDMCC No：60378，提议的分类学名称：Cystobasidium oligophagu，保藏单位地址：广东省广州市越秀区先烈中路100号大院实验大楼5楼。The high cadmium-resistant strain QN-3 was deposited in the Guangdong Provincial Microbial Culture Collection Center (GDMCC) on May 28, 2018, and the culture collection number is GDMCC No: 60378, and the proposed taxonomic name: Cystobasidium oligophagu, depositary unit Address: 5th Floor, Experimental Building, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province.

所述土壤修复剂的制备方法包括以下步骤：The preparation method of the soil remediation agent comprises the following steps:

(1)微生物菌剂的制备：在无菌条件下，经过灭菌的容器中，高耐镉菌株QN-3按照液体培养基质量的4.5％接种到液体培养基中，于22-25℃下振荡培养3天，得到种子菌液，然后将种子菌液以9％的接种量接种到液体培养基中，于28-30℃下振荡培养3-5天得到发酵菌液，直接冷冻干燥得到微生物菌剂。(1) Preparation of microbial inoculum: Under sterile conditions, in a sterilized container, the high cadmium-resistant strain QN-3 was inoculated into the liquid medium according to 4.5% of the quality of the liquid medium, and the temperature was 22-25°C. Shake culture for 3 days to obtain seed bacteria liquid, then inoculate the seed bacteria liquid with 9% of the inoculum into the liquid medium, shake culture at 28-30 ° C for 3-5 days to obtain fermentation bacteria liquid, and directly freeze-dry to obtain microorganisms Bacterial agent.

(2)复合粉剂的制备：按照重量份数依次称取改性凹凸捧土、硅藻土、农作物秸秆、家禽牲畜粪便和糖渣，分别将上述原料置于粉碎机中粉碎，随后一起置于搅拌釜中搅拌均匀，放置3-5天后烘干粉碎过筛，即得复合粉剂。(2) Preparation of composite powder: Weigh modified bump clay, diatomite, crop straw, poultry and livestock manure and sugar residue in turn according to parts by weight, place the above-mentioned raw materials in a pulverizer and pulverize them together, and then place them together in a pulverizer. Stir evenly in the stirring tank, put it for 3-5 days, dry, pulverize and sieve to obtain composite powder.

其中，所述改性凹凸捧土的制备方法：称取一定量的凹凸捧土，加入质量分数为0.1-0.2％的聚乙烯吡咯烷酮溶液和质量分数为0.2-0.3％的硼氢化钠溶液，搅拌均匀后在80-120℃下保温8-12分钟，取出冷却，然后加入白醋，混匀，最后放入冷冻干燥机干燥成固体，将固体粉碎成粉末，即得改性凹凸捧土。Wherein, the preparation method of the modified attapulgite: weighing a certain amount of attapulgite, adding a polyvinylpyrrolidone solution with a mass fraction of 0.1-0.2% and a sodium borohydride solution with a mass fraction of 0.2-0.3%, stirring After uniform, keep the temperature at 80-120°C for 8-12 minutes, take out and cool, then add white vinegar, mix well, and finally put it into a freeze dryer to dry into a solid, and pulverize the solid into powder to obtain modified attapulgite.

所述家禽牲畜粪便为猪粪、牛粪、羊粪、鸡粪或鸭粪中的任意一种或者多种，所述农作物秸秆为小麦秸秆、棉花秸秆、玉米秸秆或大豆秸秆中的任意一种或者多种。The poultry and livestock manure is any one or more of pig manure, cow manure, sheep manure, chicken manure or duck manure, and the crop straw is any one of wheat straw, cotton straw, corn straw or soybean straw or more.

所述土壤修复剂的使用方法为：在镉污染严重的土壤表面平铺一层复合粉剂，形成厚度为5-8mm的复合粉剂层，然后向复合粉剂层上均匀的喷洒微生物菌剂，翻耕混匀并补水，放置一段时间，期间定期洒水保持土壤的含水量并定期搅拌土壤；1kg复合粉剂上喷洒的微生物菌剂的重量为 100-200g。The use method of the soil remediation agent is as follows: laying a layer of composite powder on the soil surface with serious cadmium pollution to form a composite powder layer with a thickness of 5-8 mm, then evenly spraying the microbial inoculum on the composite powder layer, and ploughing. Mix well and replenish water, leave it for a period of time, sprinkle water regularly to maintain the water content of the soil and stir the soil regularly; the weight of the microbial inoculum sprayed on 1kg of the composite powder is 100-200g.

实施例1Example 1

本实施例按照本发明的方法制备该土壤修复剂，实施例1的复合粉剂其成分以重量份数计算含有：改性凹凸捧土50份、硅藻土12份、农作物秸秆20份、家禽牲畜粪便15份和糖渣5份；使用时复合粉剂层的厚度为5mm，1kg复合粉剂上喷洒的微生物菌剂的重量为100g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 1 contains in parts by weight: 50 parts of modified bump clay, 12 parts of diatomaceous earth, 20 parts of crop straw, poultry and livestock 15 parts of feces and 5 parts of sugar residue; the thickness of the composite powder layer during use is 5mm, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 100g.

实施例2Example 2

本实施例按照本发明的方法制备该土壤修复剂，实施例2的复合粉剂其成分以重量份数计算含有：改性凹凸捧土65份、硅藻土16份、农作物秸秆23份、家禽牲畜粪便16.5份和糖渣6.5份；使用时复合粉剂层的厚度为5mm，1kg复合粉剂上喷洒的微生物菌剂的重量为100g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 2 contains in parts by weight: 65 parts of modified concave-convex clay, 16 parts of diatomaceous earth, 23 parts of crop straw, poultry and livestock 16.5 parts of feces and 6.5 parts of sugar residue; the thickness of the composite powder layer is 5mm when in use, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 100g.

实施例3Example 3

本实施例按照本发明的方法制备该土壤修复剂，实施例2的复合粉剂其成分以重量份数计算含有：改性凹凸捧土80份、硅藻土20份、农作物秸秆26份、家禽牲畜粪便22份和糖渣8份；使用时复合粉剂层的厚度为 5mm，1kg复合粉剂上喷洒的微生物菌剂的重量为100g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 2 contains in parts by weight: 80 parts of modified bump clay, 20 parts of diatomaceous earth, 26 parts of crop straw, poultry and livestock 22 parts of feces and 8 parts of sugar residue; the thickness of the composite powder layer during use is 5mm, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 100g.

实施例4Example 4

本实施例按照本发明的方法制备该土壤修复剂，实施例2的复合粉剂其成分以重量份数计算含有：改性凹凸捧土80份、硅藻土20份、农作物秸秆26份、家禽牲畜粪便22份和糖渣8份；使用时复合粉剂层的厚度为 8mm，1kg复合粉剂上喷洒的微生物菌剂的重量为100g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 2 contains in parts by weight: 80 parts of modified bump clay, 20 parts of diatomaceous earth, 26 parts of crop straw, poultry and livestock 22 parts of feces and 8 parts of sugar residue; the thickness of the composite powder layer during use is 8mm, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 100g.

实施例5Example 5

本实施例按照本发明的方法制备该土壤修复剂，实施例2的复合粉剂其成分以重量份数计算含有：改性凹凸捧土80份、硅藻土20份、农作物秸秆26份、家禽牲畜粪便22份和糖渣8份；使用时复合粉剂层的厚度为 5mm，1kg复合粉剂上喷洒的微生物菌剂的重量为200g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 2 contains in parts by weight: 80 parts of modified bump clay, 20 parts of diatomaceous earth, 26 parts of crop straw, poultry and livestock 22 parts of feces and 8 parts of sugar residue; the thickness of the composite powder layer during use is 5mm, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 200g.

实施例6Example 6

本实施例按照本发明的方法制备该土壤修复剂，实施例2的复合粉剂其成分以重量份数计算含有：改性凹凸捧土80份、硅藻土20份、农作物秸秆26份、家禽牲畜粪便22份和糖渣8份；使用时复合粉剂层的厚度为 8mm，1kg复合粉剂上喷洒的微生物菌剂的重量为200g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 2 contains in parts by weight: 80 parts of modified bump clay, 20 parts of diatomaceous earth, 26 parts of crop straw, poultry and livestock 22 parts of feces and 8 parts of sugar residue; the thickness of the composite powder layer during use is 8mm, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 200g.

实施例7Example 7

本实施例按照本发明的方法制备该土壤修复剂，实施例2的复合粉剂其成分以重量份数计算含有：改性凹凸捧土80份、硅藻土20份、农作物秸秆26份、家禽牲畜粪便22份和糖渣8份；使用时复合粉剂层的厚度为 5mm，1kg复合粉剂上喷洒的微生物菌剂的重量为0g。In this example, the soil remediation agent is prepared according to the method of the present invention. The composite powder of Example 2 contains in parts by weight: 80 parts of modified bump clay, 20 parts of diatomaceous earth, 26 parts of crop straw, poultry and livestock 22 parts of feces and 8 parts of sugar residue; the thickness of the composite powder layer during use is 5mm, and the weight of the microbial inoculum sprayed on 1kg of the composite powder is 0g.

效果例：Example of effect:

(1)实验样品：本发明实施例1至7所制得的土壤修复剂。(1) Experimental sample: the soil remediation agents prepared in Examples 1 to 7 of the present invention.

(2)实验方法：重金属污染土壤：7亩重度镉污染土壤选自江苏省徐州市泉旺头村。(2) Experimental method: Heavy metal-contaminated soil: 7 mu of heavily cadmium-contaminated soil was selected from Quwangtou Village, Xuzhou City, Jiangsu Province.

(a)按照标准GB/T 23739-2009《土壤质量有效态铅和镉的测定原子吸收法》和《土壤环境监测技术规范》中的检测方法测定重金属污染土壤各种形态的镉含量；(a) Determination of cadmium content in various forms of heavy metal-contaminated soil in accordance with the detection methods in the standard GB/T 23739-2009 "Determination of Effective Lead and Cadmium in Soil Quality by Atomic Absorption Method" and "Technical Specifications for Soil Environmental Monitoring";

(b)将7亩重金属污染土壤平均分成七组，按照使用方法将七组实验样品分别施加在重金属污染土壤，翻耕混匀后得混合土壤，分别向混合土壤中加水，使混合土壤的含水量超过50％，在混合土壤上覆盖塑料薄膜，养护10天，使混合土壤中的修复剂与镉充分反应，充分稳定化，分别测定 7组混合土壤中各种形态镉的含量，测定结果见表1。(b) Divide 7 mu of heavy metal-contaminated soil into seven groups on average, and apply the seven groups of experimental samples to the heavy-metal-contaminated soil respectively according to the method of use. After ploughing and mixing, the mixed soil is obtained, and water is added to the mixed soil respectively to make the mixed soil contain When the water content exceeds 50%, the mixed soil is covered with plastic film and cured for 10 days, so that the remediation agent in the mixed soil can fully react with cadmium and fully stabilized. Table 1.

表1修复前后土壤镉形态变化统计(mg/kg)Table 1 Statistics of soil cadmium speciation before and after restoration (mg/kg)

(3)实验结果：从表1中可以看出，经过本发明实施例1至5所制得土壤修复剂处理过的重金属污染土壤中有效态镉的含量均满足GB 15618-1995《土壤环境质量标准》，而经过实施例7生产的土壤修复剂处理过的重金属污染土壤中有效态镉的含量较高，未达到GB15618-1995《土壤环境质量标准》的要求，实验结果表明，本发明实施例1至6所制得的土壤修复剂对重金属污染土壤具有很好的治理修复效果。(3) experimental results: as can be seen from Table 1, the content of available cadmium in the heavy metal polluted soil processed by the soil remediation agents prepared in Examples 1 to 5 of the present invention all satisfies GB 15618-1995 "Soil Environmental Quality" Standard ", and the content of available cadmium in the heavy metal polluted soil processed by the soil remediation agent produced in Example 7 is higher, does not meet the requirements of GB15618-1995 "Soil Environmental Quality Standard", the experimental results show that the embodiment of the present invention The soil remediation agents prepared from 1 to 6 have a good treatment and remediation effect on heavy metal polluted soil.

以上内容仅仅是对本发明构思所作的举例和说明，所属本技术领域的技术人员对所描述的具体实施例做各种各样的修改或补充或采用类似的方式替代，只要不偏离发明的构思或者超越本权利要求书所定义的范围，均应属于本发明的保护范围。The above contents are only examples and descriptions of the inventive concept. Those skilled in the art can make various modifications or supplements to the described specific embodiments or replace them in similar ways, as long as they do not deviate from the inventive concept or Anything beyond the scope defined by the claims shall belong to the protection scope of the present invention.

Claims (7)

Translated fromChinese

1.一种基于高耐镉菌株的土壤修复剂，其特征在于：它是由以下重量份的原料制成：微生物菌剂和复合粉剂，所述微生物菌剂采用由污染土壤中分离出的高耐镉菌株QN-3制成，所述复合粉剂其成分以重量份数计算，含有：改性凹凸捧土50-80份、硅藻土12-20份、农作物秸秆20-26份、家禽牲畜粪便15-22份和糖渣5-8份。1. a soil remediation agent based on high cadmium-resistant bacterial strains, is characterized in that: it is made up of the raw material of following parts by weight: microbial inoculum and composite powder, and described microbial inoculum adopts the high-efficiency microbial inoculum separated from polluted soil. The compound powder is made of cadmium-resistant strain QN-3. The composition of the composite powder is calculated in parts by weight, and contains: 50-80 parts of modified concave-convex clay, 12-20 parts of diatomaceous earth, 20-26 parts of crop straw, poultry and livestock 15-22 parts of feces and 5-8 parts of sugar residue.2.根据权利要求1所述的一种基于高耐镉菌株的土壤修复剂，其特征在于：所述高耐镉菌株QN-3于2018年05月28日保藏于广东省微生物菌种保藏中心(GDMCC)，菌种保藏号为GDMCC No：60378。2. a kind of soil remediation agent based on high cadmium tolerance bacterial strain according to claim 1, is characterized in that: described high cadmium tolerance bacterial strain QN-3 is preserved in Guangdong Province Microorganism Culture Collection Center on May 28, 2018 (GDMCC), the strain collection number is GDMCC No: 60378.3.根据权利要求1所述的一种基于高耐镉菌株的土壤修复剂，其特征在于：所述改性凹凸捧土的制备方法：称取一定量的凹凸捧土，加入质量分数为0.1-0.2％的聚乙烯吡咯烷酮溶液和质量分数为0.2-0.3％的硼氢化钠溶液，搅拌均匀后在80-120℃下保温8-12分钟，取出冷却，然后加入白醋，混匀，最后放入冷冻干燥机干燥成固体，将固体粉碎成粉末，即得改性凹凸捧土。3. a kind of soil remediation agent based on high cadmium-resistant strain according to claim 1, is characterized in that: the preparation method of described modified attapulgite: take a certain amount of attapulgite, add mass fraction to be 0.1 -0.2% polyvinylpyrrolidone solution and 0.2-0.3% sodium borohydride solution by mass fraction, stir evenly, keep at 80-120°C for 8-12 minutes, take out and cool, then add white vinegar, mix well, and finally put in Freeze dryer to dry into solid, and pulverize the solid into powder to obtain modified attapulgite.4.根据权利要求1所述的一种基于高耐镉菌株的土壤修复剂，其特征在于：所述土壤修复剂的制备方法包括以下步骤：4. a kind of soil remediation agent based on high cadmium tolerance strain according to claim 1, is characterized in that: the preparation method of described soil remediation agent comprises the following steps:(1)微生物菌剂的制备：在无菌条件下，经过灭菌的容器中，高耐镉菌株QN-3按照液体培养基质量的4.5％接种到液体培养基中，于22-25℃下振荡培养3天，得到种子菌液，然后将种子菌液以9％的接种量接种到液体培养基中，于28-30℃下振荡培养3-5天得到发酵菌液，直接冷冻干燥得到微生物菌剂。(1) Preparation of microbial inoculum: Under sterile conditions, in a sterilized container, the high cadmium-resistant strain QN-3 was inoculated into the liquid medium according to 4.5% of the quality of the liquid medium, and the temperature was 22-25°C. Shake culture for 3 days to obtain seed bacteria liquid, then inoculate the seed bacteria liquid with 9% of the inoculum into the liquid medium, shake culture at 28-30 ° C for 3-5 days to obtain fermentation bacteria liquid, and directly freeze-dry to obtain microorganisms Bacterial agent.(2)复合粉剂的制备：按照重量份数依次称取改性凹凸捧土、硅藻土、农作物秸秆、家禽牲畜粪便和糖渣，分别将上述原料置于粉碎机中粉碎，随后一起置于搅拌釜中搅拌均匀，放置3-5天后烘干粉碎过筛，即得复合粉剂。(2) Preparation of composite powder: Weigh modified bump clay, diatomite, crop straw, poultry and livestock manure and sugar residue in turn according to parts by weight, place the above-mentioned raw materials in a pulverizer and pulverize them together, and then place them together in a pulverizer. Stir evenly in the stirring tank, put it for 3-5 days, dry, pulverize and sieve to obtain composite powder.5.根据权利要求4所述的一种基于高耐镉菌株的土壤修复剂，其特征在于：所述家禽牲畜粪便为猪粪、牛粪、羊粪、鸡粪或鸭粪中的任意一种或者多种，所述农作物秸秆为小麦秸秆、棉花秸秆、玉米秸秆或大豆秸秆中的任意一种或者多种。5. a kind of soil remediation agent based on high cadmium-resistant bacterial strain according to claim 4, is characterized in that: described poultry and livestock manure is any one in pig manure, cow manure, sheep manure, chicken manure or duck manure Or more, the crop straw is any one or more of wheat straw, cotton straw, corn straw or soybean straw.6.根据权利要求1所述的一种基于高耐镉菌株的土壤修复剂，其特征在于：所述土壤修复剂的使用方法为：在镉污染严重的土壤表面平铺一层复合粉剂，形成厚度为5-8mm的复合粉剂层，然后向复合粉剂层上均匀的喷洒微生物菌剂，翻耕混匀并补水，放置一段时间，期间定期洒水保持土壤的含水量并定期搅拌土壤。6. a kind of soil remediation agent based on high cadmium-resistant strain according to claim 1, is characterized in that: the using method of described soil remediation agent is: on the soil surface with serious cadmium pollution, spread a layer of composite powder, form The composite powder layer with a thickness of 5-8mm is then sprayed with microbial inoculants evenly on the composite powder layer, ploughed, mixed and replenished with water, and left for a period of time. During this period, water is regularly sprinkled to maintain the moisture content of the soil and the soil is stirred regularly.7.根据权利要求6所述的一种基于高耐镉菌株的土壤修复剂，其特征在于：1kg复合粉剂上喷洒的微生物菌剂的重量为100-200g。7. a kind of soil remediation agent based on high cadmium tolerance strain according to claim 6 is characterized in that: the weight of the microbial inoculum sprayed on the 1kg composite powder is 100-200g.