CN111638323A - Wearable human breath product adsorption device and method - Google Patents
Wearable human breath product adsorption device and methodDownload PDFInfo
- Publication number
- CN111638323A CN111638323ACN202010430143.1ACN202010430143ACN111638323ACN 111638323 ACN111638323 ACN 111638323ACN 202010430143 ACN202010430143 ACN 202010430143ACN 111638323 ACN111638323 ACN 111638323A
- Authority
- CN
- China
- Prior art keywords
- solid
- mask
- adsorption module
- phase adsorption
- human body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001179sorption measurementMethods0.000titleclaimsabstractdescription82
- 238000000034methodMethods0.000titleclaimsabstractdescription21
- 239000007790solid phaseSubstances0.000claimsabstractdescription66
- 238000004458analytical methodMethods0.000claimsabstractdescription10
- 238000004949mass spectrometryMethods0.000claimsdescription12
- 239000000203mixtureSubstances0.000claimsdescription8
- 239000007787solidSubstances0.000claimsdescription8
- 239000012491analyteSubstances0.000claimsdescription7
- 239000004205dimethyl polysiloxaneSubstances0.000claimsdescription7
- 235000013870dimethyl polysiloxaneNutrition0.000claimsdescription7
- 229920000435poly(dimethylsiloxane)Polymers0.000claimsdescription7
- -1polydimethylsiloxanePolymers0.000claimsdescription7
- 238000004587chromatography analysisMethods0.000claimsdescription6
- 239000011248coating agentSubstances0.000claimsdescription4
- 238000000576coating methodMethods0.000claimsdescription4
- OKTJSMMVPCPJKN-UHFFFAOYSA-NCarbonChemical compound[C]OKTJSMMVPCPJKN-UHFFFAOYSA-N0.000claimsdescription3
- 239000004743PolypropyleneSubstances0.000claimsdescription3
- 229910052799carbonInorganic materials0.000claimsdescription3
- 239000002131composite materialSubstances0.000claimsdescription3
- 239000000835fiberSubstances0.000claimsdescription3
- 239000000463materialSubstances0.000claimsdescription3
- 239000002808molecular sieveSubstances0.000claimsdescription3
- 238000000465mouldingMethods0.000claimsdescription3
- CXQXSVUQTKDNFP-UHFFFAOYSA-NoctamethyltrisiloxaneChemical compoundC[Si](C)(C)O[Si](C)(C)O[Si](C)(C)CCXQXSVUQTKDNFP-UHFFFAOYSA-N0.000claimsdescription3
- 238000004987plasma desorption mass spectroscopyMethods0.000claimsdescription3
- 229920001155polypropylenePolymers0.000claimsdescription3
- URGAHOPLAPQHLN-UHFFFAOYSA-Nsodium aluminosilicateChemical compound[Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=OURGAHOPLAPQHLN-UHFFFAOYSA-N0.000claimsdescription3
- 125000004079stearyl groupChemical group[H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H]0.000claimsdescription3
- 239000003292glueSubstances0.000claimsdescription2
- PPBRXRYQALVLMV-UHFFFAOYSA-NStyreneChemical compoundC=CC1=CC=CC=C1PPBRXRYQALVLMV-UHFFFAOYSA-N0.000claims1
- 230000000903blocking effectEffects0.000claims1
- 230000008878couplingEffects0.000claims1
- 238000010168coupling processMethods0.000claims1
- 238000005859coupling reactionMethods0.000claims1
- 230000029058respiratory gaseous exchangeEffects0.000claims1
- 238000002414normal-phase solid-phase extractionMethods0.000abstractdescription9
- 238000005516engineering processMethods0.000abstractdescription7
- 230000001575pathological effectEffects0.000abstractdescription5
- 238000001514detection methodMethods0.000description8
- 210000001331noseAnatomy0.000description8
- 210000003928nasal cavityAnatomy0.000description6
- 238000001228spectrumMethods0.000description5
- 239000000443aerosolSubstances0.000description4
- RYYVLZVUVIJVGH-UHFFFAOYSA-NcaffeineChemical compoundCN1C(=O)N(C)C(=O)C2=C1N=CN2CRYYVLZVUVIJVGH-UHFFFAOYSA-N0.000description4
- 239000012071phaseSubstances0.000description4
- 238000010223real-time analysisMethods0.000description4
- VOVIALXJUBGFJZ-KWVAZRHASA-NBudesonideChemical compoundC1CC2=CC(=O)C=C[C@]2(C)[C@@H]2[C@@H]1[C@@H]1C[C@H]3OC(CCC)O[C@@]3(C(=O)CO)[C@@]1(C)C[C@@H]2OVOVIALXJUBGFJZ-KWVAZRHASA-N0.000description3
- 229960004436budesonideDrugs0.000description3
- 239000012535impuritySubstances0.000description3
- 239000007921spraySubstances0.000description3
- 238000012360testing methodMethods0.000description3
- SNICXCGAKADSCV-JTQLQIEISA-N(-)-NicotineChemical compoundCN1CCC[C@H]1C1=CC=CN=C1SNICXCGAKADSCV-JTQLQIEISA-N0.000description2
- LPHGQDQBBGAPDZ-UHFFFAOYSA-NIsocaffeineNatural productsCN1C(=O)N(C)C(=O)C2=C1N(C)C=N2LPHGQDQBBGAPDZ-UHFFFAOYSA-N0.000description2
- 241000208125NicotianaSpecies0.000description2
- 235000002637Nicotiana tabacumNutrition0.000description2
- 239000003463adsorbentSubstances0.000description2
- 229960001948caffeineDrugs0.000description2
- VJEONQKOZGKCAK-UHFFFAOYSA-NcaffeineNatural productsCN1C(=O)N(C)C(=O)C2=C1C=CN2CVJEONQKOZGKCAK-UHFFFAOYSA-N0.000description2
- 238000010586diagramMethods0.000description2
- 229940079593drugDrugs0.000description2
- 239000003814drugSubstances0.000description2
- 230000005183environmental healthEffects0.000description2
- 238000004817gas chromatographyMethods0.000description2
- 229920000779poly(divinylbenzene)Polymers0.000description2
- 238000002470solid-phase micro-extractionMethods0.000description2
- UIKROCXWUNQSPJ-VIFPVBQESA-N(-)-cotinineChemical compoundC1CC(=O)N(C)[C@@H]1C1=CC=CN=C1UIKROCXWUNQSPJ-VIFPVBQESA-N0.000description1
- UIKROCXWUNQSPJ-UHFFFAOYSA-NCotinineNatural productsC1CC(=O)N(C)C1C1=CC=CN=C1UIKROCXWUNQSPJ-UHFFFAOYSA-N0.000description1
- 238000003759clinical diagnosisMethods0.000description1
- 229950006073cotinineDrugs0.000description1
- 230000007812deficiencyEffects0.000description1
- WBGKWQHBNHJJPZ-LECWWXJVSA-NdesonideChemical compoundC1CC2=CC(=O)C=C[C@]2(C)[C@@H]2[C@@H]1[C@@H]1C[C@H]3OC(C)(C)O[C@@]3(C(=O)CO)[C@@]1(C)C[C@@H]2OWBGKWQHBNHJJPZ-LECWWXJVSA-N0.000description1
- 229960003662desonideDrugs0.000description1
- 230000035622drinkingEffects0.000description1
- 238000009513drug distributionMethods0.000description1
- 230000000694effectsEffects0.000description1
- 210000004177elastic tissueAnatomy0.000description1
- 230000003862health statusEffects0.000description1
- 230000004060metabolic processEffects0.000description1
- 229960002715nicotineDrugs0.000description1
- SNICXCGAKADSCV-UHFFFAOYSA-NnicotineNatural productsCN1CCCC1C1=CC=CN=C1SNICXCGAKADSCV-UHFFFAOYSA-N0.000description1
- 238000007781pre-processingMethods0.000description1
- 238000002203pretreatmentMethods0.000description1
- 239000010453quartzSubstances0.000description1
- 238000005070samplingMethods0.000description1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-Nsilicon dioxideInorganic materialsO=[Si]=OVYPSYNLAJGMNEJ-UHFFFAOYSA-N0.000description1
- 230000000391smoking effectEffects0.000description1
- 238000005507sprayingMethods0.000description1
- 239000000126substanceSubstances0.000description1
Images
Classifications
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D13/00—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches
- A41D13/05—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches protecting only a particular body part
- A41D13/11—Protective face masks, e.g. for surgical use, or for use in foul atmospheres
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physical Education & Sports Medicine (AREA)
- Textile Engineering (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Translated fromChinese技术领域technical field
本发明涉及人体呼出物的富集装置与分析技术领域，具体涉及一种佩戴式人体呼出物的吸附装置与分析方法。The invention relates to the technical field of enrichment devices and analysis of human exhaled matter, in particular to a wearable adsorption device and analysis method of human exhaled matter.
背景技术Background technique
人体呼出物含有大量人体生理病理代谢的分子信息。人体呼出物的检测为人体健康状态的理解与临床诊断提供了重要的科学依据。人体呼出物的分析检测通常涉及到呼气的收集与预处理，例如采用专门的呼气采集袋和采集瓶，这些专门的呼气收集装置通常较为昂贵和较大体积，难以适用于日常长时间和大批量的呼气收集。固相微萃取技术是最常用的样品预处理方式之一。固相萃取技术是通过石英纤维上的固相萃取涂层对样品中的待测物质进行吸附、富集，富集样品后的固相萃取模块与其他分析仪器如色谱或质谱进行联用实现对样品进行检测。Human exhalation contains a large amount of molecular information of human physiological and pathological metabolism. The detection of human exhalation provides an important scientific basis for the understanding and clinical diagnosis of human health status. The analysis and detection of human exhalation usually involves the collection and preprocessing of exhaled breath, such as the use of special expiratory collection bags and collection bottles. These specialized expiratory collection devices are usually expensive and large in size, making it difficult to apply for a long time in daily life. and large batches of exhaled collections. Solid-phase microextraction is one of the most commonly used sample pretreatment methods. The solid-phase extraction technology is to adsorb and enrich the substance to be tested in the sample through the solid-phase extraction coating on the quartz fiber, and the solid-phase extraction module after enriching the sample is combined with other analytical instruments such as chromatography or mass spectrometry. sample for testing.
然而对于人体呼气实时富集，当前固相萃取技术不能对气体进行直接实时采样，固相萃取需要通过顶空的方式对收集的样品进行富集，例如先使用采气袋和采气瓶收集口鼻呼出物等，再将固相微萃取模块置入气体收集装置进行富集，操作繁琐且耗时费力。However, for the real-time enrichment of human breath, the current solid-phase extraction technology cannot directly sample the gas in real time. The solid-phase extraction needs to enrich the collected samples by means of headspace, for example, using gas collection bags and gas collection bottles first. Oral and nasal exhalation, etc., and then put the solid-phase microextraction module into the gas collection device for enrichment, which is cumbersome and time-consuming.
因此，发明一种佩戴式人体呼出物吸附装置与方法来解决上述问题很有必要。Therefore, it is necessary to invent a wearable human exhaled matter adsorption device and method to solve the above problems.
发明内容SUMMARY OF THE INVENTION
本发明的目的是提供一种佩戴式人体呼出物吸附装置与方法，通过使用可佩戴式口罩作为固相吸附模块的承载体，可以过滤外部环境中的颗粒杂质，并有效收集吸附口鼻呼出物并对其进行有效的检测，突破了固相萃取技术不能对气体进行直接实时采样以及操作繁琐耗时的方式，实现了口罩内呼气的富集与分析，通过分析收集的人体呼气的成分，获得人体的生理与病理信息，以解决技术中的上述不足之处。The purpose of the present invention is to provide a wearable human body exhalation adsorption device and method. By using a wearable mask as the carrier of the solid-phase adsorption module, the particulate impurities in the external environment can be filtered, and the oral and nasal exhalation can be effectively collected and adsorbed. And effectively detect it, breaking through the method that solid-phase extraction technology cannot directly sample gas in real time and the operation is cumbersome and time-consuming, and realizes the enrichment and analysis of exhaled breath in the mask. , to obtain the physiological and pathological information of the human body to solve the above deficiencies in the technology.
为了实现上述目的，本发明提供如下技术方案：一种佩戴式人体呼出物吸附装置，包括口罩本体，所述口罩本体两侧均设有口罩带，两个所述口罩带之间连接有口罩片，所述口罩片内部设有固定器，所述固定器接有固相吸附模块；In order to achieve the above purpose, the present invention provides the following technical solutions: a wearable human exhalation adsorption device, comprising a mask body, both sides of the mask body are provided with mask straps, and a mask sheet is connected between the two mask straps , a holder is provided inside the mask sheet, and the holder is connected with a solid-phase adsorption module;
所述口罩片由纤维模块制成；The mask sheet is made of fiber modules;
所述固相吸附模块设置为片状、针状、柱状等固体成型结构，且固相吸附模块由表面疏松多孔并且具有活性基团的吸附剂和固相模块制成。The solid-phase adsorption module is arranged in a solid molding structure such as sheet, needle, column, etc., and the solid-phase adsorption module is made of an adsorbent and a solid-phase module with loose and porous surfaces and active groups.
优选的，所述固相吸附模块具体组成为聚二甲基硅氧烷PDMS、碳分子筛CAR、聚二乙烯基苯DVB、十八烷基C18和聚丙烯PA中的单种或多种复合涂层材料构成的固体模块。Preferably, the solid phase adsorption module is specifically composed of a single or multiple composite coating of polydimethylsiloxane PDMS, carbon molecular sieve CAR, polydivinylbenzene DVB, octadecyl C18 and polypropylene PA Solid modules made of layered materials.
优选的，所述口罩片位于口罩带两端之间设有弹性折叠，所述口罩带由弹性纤维纱带制成。Preferably, the mask sheet is provided with elastic folds between the two ends of the mask strap, and the mask strap is made of elastic fiber gauze tape.
优选的，所述固定器由固定夹或固体胶模块制成。Preferably, the holder is made of a fixing clip or a solid glue module.
一种佩戴式人体呼出物吸附方法，具体使用方法如下：A wearable human body exhalation adsorption method, the specific use method is as follows:
步骤一：将准备好的固相吸附模块接入固定器，整理好固相吸附模块位置后，将口罩本体戴在人体面部；Step 1: Connect the prepared solid-phase adsorption module to the holder, and after arranging the position of the solid-phase adsorption module, put the mask body on the human face;
步骤二：富集人体呼出物，在口罩片内侧面的口鼻前的空间区域呼出物被固相吸附模块所富集；Step 2: enrich the exhaled body of the human body, and the exhaled matter in the space area in front of the mouth and nose on the inner side of the mask sheet is enriched by the solid phase adsorption module;
步骤三：检测人体呼出物，将固相吸附模块取出与分析仪器耦合，对分析物进行分解吸附后，测定所吸附样品的组成及含量，将富集样品后的固相吸附模块与气相色谱或质谱联用，直接获取成分的信息。Step 3: Detect human exhaled matter, take out the solid-phase adsorption module and couple it with the analytical instrument, after decomposing and adsorbing the analyte, determine the composition and content of the adsorbed sample, and connect the enriched solid-phase adsorption module with gas chromatography or gas chromatography or Mass spectrometry is used to directly obtain information on components.
优选的，所述步骤一中口罩片为KN95标准口罩。Preferably, in the
优选的，所述步骤三中固相吸附模块吸附时间设置为0.5-4h。Preferably, in the third step, the adsorption time of the solid phase adsorption module is set to 0.5-4h.
在上述技术方案中，本发明提供的技术效果和优点：In the above-mentioned technical scheme, the technical effects and advantages provided by the present invention:
使用可佩戴式口罩作为固相吸附模块的承载体，可以过滤外部环境中的颗粒杂质，并有效收集吸附口鼻呼出物并对其进行有效的检测，突破了固相萃取技术不能对气体进行直接实时采样以及操作繁琐耗时的方式，实现了口罩内呼气的富集与分析，通过分析收集的人体呼气的成分，获得人体的生理与病理信息，在生物医药、临床分析和环境健康等领域具有广阔的应用前景。Using the wearable mask as the carrier of the solid-phase adsorption module can filter the particulate impurities in the external environment, and effectively collect and absorb the exhaled breath from the mouth and nose and perform effective detection on it, breaking through the fact that solid-phase extraction technology cannot directly detect the gas. Real-time sampling and tedious and time-consuming methods realize the enrichment and analysis of the exhaled breath in the mask. By analyzing the collected components of human exhalation, the physiological and pathological information of the human body can be obtained, which can be used in biomedicine, clinical analysis and environmental health, etc. The field has broad application prospects.
附图说明Description of drawings
为了更清楚地说明本申请实施例或现有技术中的技术方案，下面将对实施例中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明中记载的一些实施例，对于本领域普通技术人员来讲，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only described in the present invention. For some of the embodiments, those of ordinary skill in the art can also obtain other drawings according to these drawings.
图1为本发明的口罩本体内侧示意图；Fig. 1 is the inner side schematic diagram of the mask body of the present invention;
图2为本发明口罩本体的使用场景图；Fig. 2 is the use scene diagram of the mask body of the present invention;
图3为本发明实施例1的直接实时分析质谱谱图；Fig. 3 is the direct real-time analysis mass spectrogram of the embodiment of the
图4为本发明实施例2的直接实时分析质谱谱图；Fig. 4 is the direct real-time analysis mass spectrogram of the embodiment of the
图5为本发明实施例3的直接实时分析质谱谱图；Fig. 5 is the direct real-time analysis mass spectrogram of the embodiment of the
图6为本发明实施例4的直接实时分析质谱谱图。FIG. 6 is a direct real-time analysis mass spectrogram of Example 4 of the present invention.
附图标记说明：Description of reference numbers:
1口罩带、2口罩片、3固定器、4固相吸附模块。1 mask belt, 2 mask sheets, 3 holder, 4 solid phase adsorption modules.
具体实施方式Detailed ways
为了使本领域的技术人员更好地理解本发明的技术方案，下面将结合附图对本发明作进一步的详细介绍。In order to make those skilled in the art better understand the technical solutions of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.
本发明提供了如图1-2所示的一种佩戴式人体呼出物吸附装置与方法，包括口罩本体，所述口罩本体两侧均设有口罩带1，两个所述口罩带1之间连接有口罩片2，所述口罩片2内部设有固定器3，所述固定器3接有固相吸附模块4；The present invention provides a wearable human exhaled body adsorption device and method as shown in Figures 1-2, including a mask body, and
所述固相吸附模块4设置片状、针状、柱状等固体成型结构，且固相吸附模块由表面疏松多孔并且具有活性基团的吸附剂和固相模块制成。The solid
进一步的，在上述技术方案中，所述固相吸附模块4具体组成为聚二甲基硅氧烷PDMS、碳分子筛CAR、聚二乙烯基苯DVB、十八烷基C18和聚丙烯PA中的单种或多种复合涂层模块。Further, in the above technical solution, the solid
一种佩戴式人体呼出物吸附方法，具体使用方法如下：A wearable human body exhalation adsorption method, the specific use method is as follows:
步骤一：将准备好的固相吸附模块4接入固定器3，整理好固相吸附模块4位置后，将口罩本体戴在人体面部；Step 1: Connect the prepared solid-
步骤二：富集人体呼出物，在口罩片2内侧面的口鼻前的空间区域呼出物被固相吸附模块4所富集；Step 2: enriching the exhaled matter of the human body, and the exhaled matter in the space area in front of the mouth and nose on the inner side of the
步骤三：检测人体呼出物，将固相吸附模块4取出与分析仪器耦合，对分析物进行分解吸附后，测定所吸附样品的组成及含量，将富集样品后的固相吸附模块4与气相色谱或质谱联用，直接获取成分的信息。Step 3: Detect the exhaled body of the human body, take out the solid-
进一步的，在上述技术方案中，所述步骤二中固相吸附模块4吸附时间设置为0.5-4h。Further, in the above technical solution, the adsorption time of the solid-
实施例1：Example 1:
使用本发明的装置对人体喝咖啡后10分钟后的呼气进行收集吸附，并进行口腔呼气残留物的检测，具体方法如下：The device of the present invention is used to collect and adsorb the exhalation of the human body 10 minutes after drinking coffee, and to detect the oral exhalation residue. The specific method is as follows:
步骤一：被测人饮入咖啡200ml，十分钟后，将准备好的固相吸附模块4置于固定器3，将口罩本体戴在人体面部；Step 1: The tested person drinks 200ml of coffee, ten minutes later, put the prepared solid
步骤二：富集人体呼出物，在口罩片2内侧面的口鼻前的空间区域的呼出物被固相吸附模块4所富集，富集2小时后取出固相吸附模块4进行直接实时质谱分析；Step 2: Enrich human exhaled matter, the exhaled matter in the space area in front of the mouth and nose on the inner side of the
步骤三：检测人体呼出物，将固相吸附模块4取出与分析仪器耦合，对分析物进行分解吸附后，测定所吸附样品的组成及含量，将富集样品后的固相吸附模块与气相色谱或质谱联用，直接获取成分的信息。Step 3: Detect the exhaled body of the human body, take out the solid-
本实施例检测结果如图3所示，结果表明谱图中m/z 195.09为咖啡的标志性物质咖啡因的质子峰，数据表明此装置检测到了呼气中咖啡的标志成分咖啡因。The detection results of this embodiment are shown in Figure 3. The results show that m/z 195.09 in the spectrum is the proton peak of caffeine, the symbol of coffee, and the data shows that the device detects caffeine, the symbol of coffee in the breath.
实施例2：Example 2:
使用本发明的装置在人体抽烟10秒钟后的呼气进行收集吸附，并进行口腔呼气残留物的检测，具体方法如下：The device of the present invention is used to collect and absorb the exhalation of the human body after smoking for 10 seconds, and to detect the oral exhalation residue. The specific method is as follows:
步骤一：被测人抽烟十秒钟后，将准备好的固相吸附模块4插入固定器3，整理好固相吸附模块4的位置后，将口罩本体戴在人体面部；Step 1: After the tested person smokes for ten seconds, insert the prepared solid-
步骤二：富集人体呼出物，在口罩片2内侧面的口鼻前的空间区域呼出物被固相吸附模块4所富集，富集2小时后取出固相吸附模块4进行直接实时质谱分析；Step 2: Enriching the exhaled body of the human body, the exhaled matter in the space area in front of the mouth and nose on the inner side of the
步骤三：检测人体呼出物，将固相吸附模块4取出与分析仪器耦合，对分析物进行分解吸附后，测定所吸附样品的组成及含量，将富集样品后的固相吸附模块4与气相色谱或质谱联用，直接获取成分的信息。Step 3: Detect the exhaled body of the human body, take out the solid-
本实施例检测结果如图4所示，结果表明在谱图中m/z 163.12为烟草成分尼古丁的质子峰，在谱图中m/z 177.11为烟草成分尼古丁的代谢物可替宁的质子峰，数据表明口腔呼气中的残留烟草成分能被植入口罩中的固相吸附模块采集，富集已经被检测和鉴定。The test results of this example are shown in Figure 4. The results show that m/z 163.12 in the spectrum is the proton peak of nicotine, a tobacco component, and m/z 177.11 is the proton peak of cotinine, a metabolite of nicotine, in the spectrum. , the data show that the residual tobacco components in oral exhalation can be collected by the solid-phase adsorption module implanted in the mask, and the enrichment has been detected and identified.
实施例3：Example 3:
使用本发明的装置鼻腔喷入微量赛洛唑啉气雾剂后10分钟后的呼气进行收集吸附，并进行鼻腔呼气残留物的检测，具体方法如下：Use the device of the present invention to spray into the nasal cavity of a trace amount of xylozoline aerosol to collect and absorb the breath after 10 minutes, and carry out the detection of the nasal cavity exhalation residue, and the specific method is as follows:
步骤一：被测人鼻腔喷入微量赛洛唑啉气雾剂，十分钟后，将准备好的固相吸附模块4插入固定器3，整理好固相吸附模块4的位置后，将口罩本体戴在人体面部；Step 1: Spray a small amount of xyloxazoline aerosol into the nasal cavity of the tested person. After ten minutes, insert the prepared solid-
步骤二：富集人体呼出物，在口罩片2内侧面的口鼻前的空间区域呼出物被固相吸附模块4所富集，富集2小时后取出固相吸附模块4进行直接实时质谱分析；Step 2: Enriching the exhaled body of the human body, the exhaled matter in the space area in front of the mouth and nose on the inner side of the
步骤三：检测人体呼出物，将固相吸附模块4取出与分析仪器耦合，对分析物进行分解吸附后，测定所吸附样品的组成及含量，将富集样品后的固相吸附模块4与气相色谱或质谱联用，直接获取成分的信息。Step 3: Detect the exhaled body of the human body, take out the solid-
本实施例检测结果如图5所示，结果表明在谱图中m/z 245.21为药物成分赛洛唑啉的质子峰，数据表明鼻腔呼气中的残留药物能被植入口罩中的固相吸附模块采集，富集已经被检测和鉴定。The test results of the present embodiment are shown in Figure 5. The results show that m/z 245.21 in the spectrum is the proton peak of the drug component xilozoline. The data shows that the residual drug in the nasal cavity can be implanted in the solid phase of the mask. Adsorption module acquisition, enrichment has been detected and identified.
实施例4：Example 4:
使用本发明的装置对人体鼻腔喷入微量布地奈德气雾剂10分钟后的呼气进行收集吸附，并进行鼻腔呼气残留物的检测，具体方法如下：The device of the present invention is used to collect and absorb the exhalation after 10 minutes of spraying a trace amount of budesonide aerosol into the nasal cavity of the human body, and to detect the nasal exhalation residue. The specific method is as follows:
步骤一：被测人鼻腔喷入微量布地奈德气雾剂，十分钟后，将准备好的固相吸附模块4插入固定器3，整理好固相吸附模块4位置后，将口罩本体戴在人体面部；Step 1: Spray a small amount of budesonide aerosol into the nasal cavity of the tested person. After ten minutes, insert the prepared solid-
步骤二：富集人体呼出物，在口罩片2内侧面的口鼻前的空间区域呼出物被固相吸附模块4所富集，富集2小时后取出固相吸附模块4进行直接实时质谱分析；Step 2: Enriching the exhaled body of the human body, the exhaled matter in the space area in front of the mouth and nose on the inner side of the
步骤三：检测人体呼出物，将固相吸附模块4取出与分析仪器耦合，对分析物进行分解吸附后，测定所吸附样品的组成及含量，将富集样品后的固相吸附模块4与气相色谱或质谱联用，直接获取成分的信息。Step 3: Detect the exhaled body of the human body, take out the solid-
本实施例检测结果如图6所示，结果表明在谱图中m/z 431.25为药物成分布地奈德的质子峰，数据表明呼气中的布地奈德被富集和检测被准确的鉴定。The detection results of this example are shown in Figure 6. The results show that m/z 431.25 in the spectrum is the proton peak of the drug distribution of desonide. The data shows that budesonide in the exhaled breath is enriched and accurately identified by detection.
以上实施例证明，本发明的装置能收集吸附口鼻呼出物并对其进行有效的检测，使用可佩戴式口罩作为固相吸附模块的承载体，可以过滤外部环境中的颗粒杂质，从技术上突破了固相萃取技术不能对气体进行直接实时采样以及操作繁琐耗时的方式，本发明创造性的实现了口罩内呼气的富集与分析，通过分析收集的人体呼气的成分，获得人体的生理与病理信息，在生物医药、临床分析和环境健康等领域具有广阔的应用前景。The above examples prove that the device of the present invention can collect and adsorb the exhaled material of the mouth and nose and perform effective detection on it. Using a wearable mask as the carrier of the solid-phase adsorption module can filter the particulate impurities in the external environment. Technically Breaking through the method that solid-phase extraction technology cannot directly sample the gas in real time and the operation is cumbersome and time-consuming, the present invention creatively realizes the enrichment and analysis of exhaled breath in the mask, and obtains the human body’s exhaled breath by analyzing the collected components of human exhalation. Physiological and pathological information has broad application prospects in the fields of biomedicine, clinical analysis and environmental health.
以上只通过说明的方式描述了本发明的某些示范性实施例，毋庸置疑，对于本领域的普通技术人员，在不偏离本发明的精神和范围的情况下，可以用各种不同的方式对所描述的实施例进行修正。因此，上述附图和描述在本质上是说明性的，不应理解为对本发明权利要求保护范围的限制。Certain exemplary embodiments of the present invention have been described above by way of illustration only, and it is needless to say that those skilled in the art may The described embodiments are modified. Accordingly, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of the claims of the present invention.
Claims (6)
Translated fromChinesePriority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010430143.1ACN111638323A (en) | 2020-05-20 | 2020-05-20 | Wearable human breath product adsorption device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010430143.1ACN111638323A (en) | 2020-05-20 | 2020-05-20 | Wearable human breath product adsorption device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111638323Atrue CN111638323A (en) | 2020-09-08 |
Family
ID=72332109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010430143.1APendingCN111638323A (en) | 2020-05-20 | 2020-05-20 | Wearable human breath product adsorption device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111638323A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116008518A (en)* | 2022-09-15 | 2023-04-25 | 武汉高芯科技有限公司 | A kind of mask for detection and detection method thereof |
| CN117016888A (en)* | 2020-09-21 | 2023-11-10 | 天津大学 | Expired air condensate collecting and detecting device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070199567A1 (en)* | 2006-01-25 | 2007-08-30 | Kanzer Steve H | Droplet collection devices and methods to detect and control airborne communicable diseases utilizing rfid |
| JP2013171033A (en)* | 2012-02-22 | 2013-09-02 | Minato Ikagaku Kk | Mask for respiration measurement |
| US20150068279A1 (en)* | 2013-09-11 | 2015-03-12 | King Abdulaziz City For Science And Technology | Electro-enhanced solid-phase microextraction method |
| JP2019184288A (en)* | 2018-04-03 | 2019-10-24 | 国立研究開発法人量子科学技術研究開発機構 | Gas analyzer and mask applied to the same |
| CN210445776U (en)* | 2020-02-29 | 2020-05-05 | 首都医科大学宣武医院 | Masks for sampling of novel coronavirus pneumonia pathogens |
- 2020
- 2020-05-20CNCN202010430143.1Apatent/CN111638323A/enactivePending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070199567A1 (en)* | 2006-01-25 | 2007-08-30 | Kanzer Steve H | Droplet collection devices and methods to detect and control airborne communicable diseases utilizing rfid |
| JP2013171033A (en)* | 2012-02-22 | 2013-09-02 | Minato Ikagaku Kk | Mask for respiration measurement |
| US20150068279A1 (en)* | 2013-09-11 | 2015-03-12 | King Abdulaziz City For Science And Technology | Electro-enhanced solid-phase microextraction method |
| JP2019184288A (en)* | 2018-04-03 | 2019-10-24 | 国立研究開発法人量子科学技術研究開発機構 | Gas analyzer and mask applied to the same |
| CN210445776U (en)* | 2020-02-29 | 2020-05-05 | 首都医科大学宣武医院 | Masks for sampling of novel coronavirus pneumonia pathogens |
Non-Patent Citations (1)
| Title |
|---|
| 孟品佳等译: "《分析化学中的样品制备技术》", 中国人民公安大学出版社, pages: 114 - 116* |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117016888A (en)* | 2020-09-21 | 2023-11-10 | 天津大学 | Expired air condensate collecting and detecting device |
| CN116008518A (en)* | 2022-09-15 | 2023-04-25 | 武汉高芯科技有限公司 | A kind of mask for detection and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20230125894A1 (en) | System and Method for Drug Detection in Exhaled Breath | |
| CA2924107C (en) | System and method for eluting and testing substance from exhaled aerosol sample | |
| Buszewski et al. | Human exhaled air analytics: biomarkers of diseases | |
| Schmidt et al. | Ammonia in breath and emitted from skin | |
| DE69327816T2 (en) | Device and method for examining exhaled air for clinical purposes | |
| JP5716194B2 (en) | Apparatus and method for analyzing gas and alveolar air components in the oral cavity | |
| Basanta et al. | An adaptive breath sampler for use with human subjects with an impaired respiratory function | |
| Gashimova et al. | Exhaled breath analysis using GC-MS and an electronic nose for lung cancer diagnostics | |
| JP2013504074A5 (en) | ||
| CN111638323A (en) | Wearable human breath product adsorption device and method | |
| WO2009025488A2 (en) | Apparatus and method of analyzing constituents of gas in oral cavity and alveolar gas | |
| CN103487481A (en) | Expiration ammonia gas analyzer | |
| JP2016521361A (en) | Mixed breath gas analyzer for bad breath detection and analysis method | |
| CN104939831A (en) | System and method for collecting samples in exhaled gas of subject and uses of method | |
| CN110824076A (en) | Solid phase micro-extraction swab device and method | |
| CA2098215A1 (en) | Expired gas analytical method and device | |
| JP2024515320A (en) | Gas or breath sampling device that captures both aerosol and vapor fractions | |
| WO2009147576A2 (en) | Breath collection device and method | |
| Rydosz et al. | A Novel Approach for Device Dedicated to Non-Invasive Diabetes Con-trol | |
| KR101168262B1 (en) | Method for measuring halitosis | |
| KR20210142430A (en) | Mask for Preprocessing virus diagnosis of non-contact and diagnostic apparatus using the same | |
| CN116269523B (en) | Sebum gas collection and infrared spectrum enhancement device | |
| JPS60250227A (en) | Sample gas sampling device | |
| KR101234725B1 (en) | Instrument for measuring halitosis | |
| PL236448B1 (en) | Individual portable device for monitoring of inhaled air composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20200908 | |
| RJ01 | Rejection of invention patent application after publication |