CN110903138A - Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests - Google Patents

Abstract

The invention provides a microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode diseases, clubroot diseases and underground pests. The compound fertilizer is prepared from the following raw materials of purple lilac spore bacteria, bacillus subtilis, trichoderma harzianum, total matrine, abamectin, selenium-enriched yeast, sugar alcohol composite boron, yellow humic acid calcium, amino acid, tea seed cake, cane sugar and turf. Mixing tea seed cake and turf with 50% water content and selenium-enriched yeast and cane sugar with 10% water content. Stacking and carrying out aerobic fermentation to form a mixture A; turning the mixture A, crushing, sequentially adding sugar alcohol composite boron, total matrines, amino acid raw powder and calcium fulvate, and fully and uniformly stirring to form a mixture B; and adding the bacillus subtilis, the purple lilac spore bacteria, the trichoderma harzianum and the abamectin into the mixture B, and fully and uniformly stirring to obtain the selenium-rich pesticide fertilizer. The invention can prevent and control root knot nematode disease, clubroot and underground pests. The microbial selenium-rich pesticide fertilizer is suitable for preventing and treating root knot nematode diseases, clubroot diseases and underground pests.

Description

Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests

Technical Field

The invention relates to a microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot disease and underground pests, which belongs to the field of pesticides and kills plant diseases and insect pests.

Background

At present, more than 80 kinds of root-knot nematodes are reported internationally, mainly parasitize over 20000 kinds of hosts such as vegetables, fruit trees, economic crops, grain crops, ornamental plants, weeds and the like, plasmodiophora belongs to weak parasitic fungi, namely, the plasmodiophora can survive and infect in many adverse environments, has particularly strong vitality and can survive in soil for more than 10 years.

Clubroot and root knot nematode disease are two major root diseases which are prominent in crop production, particularly vegetable production, and tumors with different sizes are formed at roots to cause root malformation, so that overground parts are finally slow in growth, short, water-deficient, fertilizer-deficient and small and old seedling states. With the progress of the disease, the leaves gradually become withered and yellow, and the death of the whole plant can be caused seriously. Because the two diseases have cross-onset and have a plurality of common parts, the two diseases are often confused by growers in production, and the two diseases cannot be effectively prevented and treated.

The host range is wide, clubroot mostly exists in cruciferae, the life cycle of the first stage can be completed in host plants of non-cruciferae, the quality and yield of vegetables are seriously damaged, the yield is reduced by 25% if the weight is reduced, and the weight is more than 85% if the weight is reduced. The host range of the root knot nematode disease is wider, more than 2000 roots are obtained, and the root knot nematode disease mainly comprises root knot nematode of melon and fruit (javnica) (harmful to tomatoes), root knot nematode of south China (incognita), root knot nematode of north China (hapla), root knot nematode of peanut (arenaria) and the like.

At present, the invention patents related to root-knot nematodes and the invention patents related to root-knot nematodes mostly consider the root-knot nematodes, particularly, the root-knot nematodes and the root-knot nematodes of cruciferous crops are cross-infectious, if the control of the root-knot nematodes is only considered, but the root-knot nematodes and the nutrition of plants are ignored, particularly, the plants are green and environment-friendly, and the later loss is serious, thus seriously threatening the survival and development of human beings.

The invention relates to a drug fertilizer for root-knot nematodes through the search and discovery of invention patents (application No. 201310578539.0), which describes a technical scheme of using chemical fertilizers such as vitamin, seaweed fertilizer, fosthiazate, urea, diammonium phosphate, ammonium sulfate, potassium sulfate and the like, the scheme can undoubtedly increase the production cost of the drug fertilizer, the product only pays attention to the prevention and treatment of the root-knot nematodes, but neglects the treatment of clubroot. The chemical fertilizer is easy to cause soil acidification and hardening after long-term use, has large residual quantity, and does not relate to the addition of beneficial flora for protecting soil.

Currently, biological enrichment and poisoning in China are serious, because pollution of chemical fertilizers and chemical pesticides seriously threatens the survival and development of human beings, people tend to enter various elements of the environment through spreading and applying the chemical fertilizers and the chemical pesticides in crop soil and water areas, and the chemical fertilizers and the chemical pesticides are diffused, adsorbed, operated, absorbed, enriched and the like. The method enters a multi-mode and multi-track operation and circulation and is distributed in all corners of the earth, and the environmental pollution loss caused by chemical fertilizers and chemical pesticides in China is over 95 hundred million, which accounts for over 25 percent of the total pollution loss. The three wastes and the urban garbage in five pollution factories of food (pesticides, fertilizers and the like) have the longest duration, and the residue has the greatest harm to human.

Based on the above problems, the present inventors have considered that there is a need for further improvement of the production formulation of medical fertilizers, which uses various characteristics of beneficial bacterial flora to inhibit harmful bacteria and insects. But also increases the plant nutrition. The present invention is not in conflict with the above-mentioned search patents.

Disclosure of Invention

Aiming at the defects of the existing pesticide fertilizer, the invention provides a microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode diseases, clubroot diseases and underground pests. The pesticide fertilizer solves the technical problem of combined preparation of pesticide fertilizers by preparing raw materials.

The technical scheme adopted by the invention for solving the technical problems is as follows:

0.5-2 parts of purple lilac spore bacteria, 0.2-1.5 parts of bacillus subtilis, 0.1-1 part of trichoderma harzianum, 0.5-1.5 parts of total matrines, 0.5-2 parts of abamectin, 0.2-1 part of selenium-enriched yeast, 0.3-1.2 parts of sugar alcohol composite boron, 15-24 parts of calcium fulvate, 12-20 parts of amino acid, 18-28 parts of tea seed cake, 3-8 parts of sucrose and 10-50 parts of turf.

Mixing tea seed cake and turf with 50% water content and selenium-enriched yeast and cane sugar with 10% water content. Stacking and carrying out aerobic fermentation to form a mixture A;

turning the mixture A, crushing, sequentially adding sugar alcohol composite boron, total matrines, amino acid raw powder and calcium fulvate, and fully and uniformly stirring to form a mixture B;

and adding the bacillus subtilis, the purple lilac spore bacteria, the trichoderma harzianum and the abamectin into the mixture B, and fully and uniformly stirring to obtain the selenium-rich pesticide fertilizer.

The positive effects are as follows: the formula of the invention is a broad-spectrum multifunctional ecological fertilizer, can be used for all crops and most of soil and climatic conditions, has wide application and main effects: the compound fertilizer can prevent and treat root knot nematode disease, clubroot disease and underground pests, improve soil, improve quality, increase yield, save manpower and material resources, is a natural antidote, and is an ecological multifunctional green and environment-friendly pesticide fertilizer for ecological organic food. The microbial selenium-rich pesticide fertilizer is suitable for preventing and treating root knot nematode diseases, clubroot diseases and underground pests.

Drawings

FIG. 1 is a diagram of the process steps of the present invention;

fig. 2 is an effect diagram of the present invention.

Detailed Description

The raw material ratio is as follows:

0.5-2 parts of purple lilac spore bacteria, 0.2-1.5 parts of bacillus subtilis, 0.1-1 part of trichoderma harzianum, 0.5-1.5 parts of total matrines, 0.5-2 parts of abamectin, 0.2-1 part of selenium-enriched yeast, 0.3-1.2 parts of sugar alcohol composite boron, 15-24 parts of calcium fulvate, 12-20 parts of amino acid, 18-28 parts of tea seed cake, 3-8 parts of sucrose and 10-50 parts of turf.

The process comprises the following steps:

firstly, uniformly mixing the tea seed cake with 50% of water and the turf, and uniformly mixing the tea seed cake with 10% of water, the selenium-enriched yeast and the cane sugar. Stacking and carrying out aerobic fermentation to form a mixture A.

And (II) turning the mixture A, crushing, sequentially adding sugar alcohol composite boron, total matrines, amino acid raw powder and calcium fulvate, and fully and uniformly stirring to form a mixture B.

And (III) adding the bacillus subtilis, the lilac purple spore bacteria, the trichoderma harzianum and the abamectin into the mixture B, and fully and uniformly stirring.

And (IV) sieving, drying, packaging and warehousing.

Example 1

The raw material ratio is as follows:

0.7 part of purple lilac spore bacteria, 0.5 part of bacillus subtilis, 0.4 part of trichoderma harzianum, 0.8 part of total matrine, 0.8 part of abamectin, 0.4 part of selenium-enriched yeast, 0.6 part of sugar alcohol composite boron, 18 parts of calcium fulvate, 14 parts of amino acid, 20 parts of tea seed cake, 4 parts of cane sugar and 40 parts of turf.

Example 2

The raw material ratio is as follows:

1 part of purple lilac spore fungus, 0.8 part of bacillus subtilis, 0.6 part of trichoderma harzianum, 1 part of total matrine, 1 part of abamectin, 0.6 part of selenium-enriched yeast, 0.8 part of sugar alcohol composite boron, 20 parts of calcium fulvate, 16 parts of amino acid, 22 parts of tea seed cake, 5 parts of cane sugar and 32 parts of turf.

Example 3

The raw material ratio is as follows:

0.5 part of purple lilac spore bacteria, 0.2 part of bacillus subtilis, 0.1 part of trichoderma harzianum, 0.5 part of total matrine, 0.5 part of abamectin, 0.2 part of selenium-enriched yeast, 0.3 part of sugar alcohol composite boron, 15 parts of calcium fulvate, 12 parts of amino acid, 18 parts of tea seed cake, 3 parts of cane sugar and 50 parts of turf.

Example 4

The raw material ratio is as follows:

1.4 parts of purple lilac spore bacteria, 1 part of bacillus subtilis, 0.8 part of trichoderma harzianum, 1.2 parts of total matrines, 1.5 parts of abamectin, 0.7 part of selenium-enriched yeast, 0.9 part of sugar alcohol composite boron, 22 parts of yellow humic acid calcium, 17 parts of amino acid, 25 parts of tea seed cake, 6 parts of cane sugar and 23 parts of grass carbon.

Example 5

The raw material ratio is as follows:

1.8 parts of purple lilac spore bacteria, 1.2 parts of bacillus subtilis, 0.9 part of trichoderma harzianum, 1.4 parts of total matrines, 1.8 parts of abamectin, 0.8 part of selenium-enriched yeast, 1 part of sugar alcohol composite boron, 23 parts of yellow humic acid calcium, 18 parts of amino acid, 27 parts of tea seed cake, 7 parts of cane sugar and 17 parts of turf.

Example 6

The raw material ratio is as follows:

2 parts of purple lilac spore fungus, 1.5 parts of bacillus subtilis, 1 part of trichoderma harzianum, 1.5 parts of total matrine, 2 parts of abamectin, 1 part of selenium-enriched yeast, 1.2 parts of sugar alcohol composite boron, 24 parts of calcium fulvate, 20 parts of amino acid, 28 parts of tea seed cake, 8 parts of cane sugar and 10 parts of turf.

The performance of the raw materials is as follows:

purple lilac spore fungus: the lilac violet spore fungus agent is a novel microorganism live spore preparation, belongs to endoparasitic fungi, is an important natural enemy of some plant parasitic nematodes, can parasitize eggs, can erode larvae and female worms, and can obviously reduce the harm of plant nematode diseases such as root-knot nematodes, cyst nematodes, stem nematodes and the like of various crops. The purple lilac spore fungus can secrete and synthesize various organic acids, enzymes, physiological active substances and the like, the effective components of the purple lilac spore fungus can germinate and generate hyphae invading the nematode eggs and the adult body walls, the components in the nematode body are absorbed to propagate in the nematode body, the normal physiological metabolism of the nematode is damaged, and finally the nematode eggs and the adult are killed. Has the advantages of high efficiency, safety, greenness, no pollution, no residue and the like.

B, bacillus subtilis: the bacillus subtilis can metabolize and secrete bacteriocin (subtilin, polymyxin, nystatin and the like) peptide lipid compounds, organic acid substances and the like in the growth process, the metabolites can effectively inhibit the growth of pathogenic bacteria or dissolve the pathogenic bacteria so as to kill the pathogenic bacteria and have high continuous cropping resistance, and the secreted enzymes of the bacillus subtilis comprise chitinase antimicrobial protein which has strong inhibition effect on various plant pathogenic bacteria (particularly on flagellaria fungi of lower fungi of cruciferous plants and independent plasmodiophora).

The bacillus subtilis forms dominant organisms and groups at the roots of crops, so that the invasion of other pathogenic bacteria is effectively prevented, the nutrition of surrounding bacteria is obtained, and the growth of flagellates and plasmodiophora is inhibited; the bacillus subtilis plays a role in resisting diseases like a vaccine. Secondly, the bacillus subtilis can grow on hyphae of flagellate subtenon fungi and plasmodiophora, pathogenic bacteria are decomposed and consumed, the pathogenic bacteria silk is broken, decomposed cytoplasm is digested, and then the pathogenic bacteria can not further infect plants, so that the effects of bacteriostasis and disease resistance are achieved.

In addition, the bacillus subtilis has two growth periods, namely a spore dormancy period and a reproductive period, can enter the spore dormancy period under unfavorable environments such as severe growth environment, lack of nutrient substances and the like, forms spores which have strong stress resistance and can survive under polar environments such as high temperature, acid and alkali and the like, so that the bacillus subtilis can survive by adapting to the environment, and once the environment becomes suitable for growth, the nutrition is sufficient, the spores automatically enter the reproductive period, and the spores grow into the bacillus subtilis again.

Trichoderma harzianum: is a pure microorganism, a broad-spectrum bactericide, and is prepared by multiple mechanisms of nutrition competition, heavy parasitism, cell wall decomposition enzyme, induction of plant resistance and the like.

Competition effect: the trichoderma harzianum can grow rapidly around the roots of plants, occupies the sites on the surface of the plants, forms a protective cover and prevents pathogenic fungi from contacting the root systems of the plants, so that the root systems of the plants are protected from being infected by pathogenic fungi.

Heavy parasitic action: refers to a complex process of identification of pathogenic bacteria, contact, entanglement, penetration and parasitism in a series of sequential steps. In the interaction process of trichoderma and pathogenic bacteria, host hyphae secrete substances to enable trichoderma to grow towards host fungi, once a host is identified by trichoderma parasites, the trichoderma hyphae grow in a spiral winding mode in parallel with the host hyphae, attached cellular branches are generated and adsorbed on the host hyphae, cell walls are dissolved through secreting extracellular enzymes, the host hyphae penetrate through the host hyphae, nutrition is absorbed, and then the pathogenic bacteria are killed.

The antibiotic action: the trichoderma harzianum can secrete part of antibiotics, can inhibit the growth and colonization of pathogenic bacteria, and can reduce the harm of the pathogenic bacteria.

Plant growth regulation: the trichoderma harzianum colonizes plant root systems and generates compounds for stimulating plant growth and inducing plant defense reaction, so that the microenvironment of the root systems is improved, and the growth vigor and disease resistance of plants are enhanced.

Matrine, and matrine: is a low-toxicity, low-residue and environment-friendly biological pesticide. The matrine is a natural plant pesticide, once the root-knot nematode touches the pesticide, the nerve centre is paralyzed, the protein of the body of the nematode is solidified, the body pore of the nematode is blocked, and the nematode is suffocated and dies.

Abamectin: is a sixteen-membered macrolide compound with insecticidal, acaricidal and nematicidal activities. The action mechanism is to interfere the physiological activity of the nerve, stimulate and release gamma-aminobutyric acid, and after the gamma-aminobutyric acid contacts with the root-knot nematode larvae, paralysis symptoms appear, and the nematode dies after being inactive and does not eat for 2 to 4 hours.

The abamectin is a high-efficiency medicament for killing various parasitic nematodes, has the second characteristic that the abamectin is different from other insecticides in medicinal property and is not easy to generate medicinal property, and has the third characteristic of safety, safety to natural enemies and less pollution to the environment.

Selenium-rich yeast: the selenium content can reach more than 1000 × 10 μ g, usually 500 × 10 μ g, its protein is greater than or equal to 40%, it is rich in 18 amino acids, and vitamin B is 33.2 × 10 μ g. Selenium in the selenium-enriched yeast replaces sulfur in the sulfur-containing amino acid to form seleno-amino acid, and further protein is formed. The biological effect is 10-20 times of that of inorganic selenium, the selenium-enriched yeast can be used as selenium source, and at the same time can provide other beneficial nutrients, it possesses high selenium-enriching capacity and capacity of converting inorganic selenium into organic selenium, and is a natural antidote capable of removing heavy metal accumulation in plant body, and selenium has strong affinity for metal, can be combined with metal to form metal-selenium-protein compound, and has the detoxication action for mercury, cadmium, lead and arsenic, etc. and can be classified according to toxicity and belong to non-toxic substance.

Sugar alcohol complex boron: the sugar alcohol is a good chelating agent for nutrients such as large, medium and trace elements, can be combined with various nutrients to form a stable complex, and is particularly higher in solubility in alkaline solution. Boron is used for preventing and treating clubroot, and different from Ca and PH values, boron can inhibit primary infection of clubroot and secondary infection of clubroot. However, the effects of boron on clubroot are mainly intracellular and therefore do not reduce the number of infected root hairs.

Calcium fulvate: is a high-content organic calcium.

1. Can adjust the pH value of soil and reduce the harm of salt substances and continuous cropping.

2. Can promote the root development of crops.

3. Improving plant immunity, and inhibiting diseases and insect pests. Can effectively control the clubroot, can directly influence the viability of the dormant spores of the clubroot, and can reduce the morbidity degree even if the dormant spores are applied after the clubroot is infected.

4. Ca is the main component of the middle layer of the cell wall, and calcium pectate in the cell wall can improve the resistance of cell wall degrading enzymes.

5. Ca is necessary for membrane structure and ion exchange, so that the cell wall and the membrane structure are stable enough, and the distribution of calmodulin in two flagella is different, thereby causing adverse reaction for changing external Ca environment, further influencing the moving direction of zoospores and inhibiting infection of plasmodiophora to a certain extent.

Amino acids: the compound fertilizer can promote photosynthesis, especially glycine can increase the chlorophyll content in plant, raise the activity of several enzymes, promote Ca absorption and utilization of crop, increase Vc content and sugar content, and has long-acting and fast-acting fertilizer supplementing effect. The polypeptide can improve the immunity of crop roots.

Tea seed cake: the pesticide contains 4.6 percent of N, P2O52.48 percent of N and 1.4 percent of K2O 1.4 percent of N, can effectively prevent snails and slugs, leeches, rice planthoppers and rice leafhoppers, can also prevent soil insects, weevils, grubs, mole crickets, wireworms and the like, and has no pollution, no residue and long pesticide effect.

Sucrose: contains multiple vitamins, folic acid, nicotinic acid, trace elements, amino acids, vitamins and antioxidant substances. Can resist free radicals, protect cell basic structure, maintain normal cell function and metabolism, and promote cell regeneration.

Grass carbon: the organic fertilizer is rich in various elements such as N, P, K, Ca, Mg and the like, is a pure natural organic matter, is a non-toxic, pollution-free and residue-free green substance, and has the capabilities of breaking soil hardening, improving soil water holding capacity, ventilating and preserving fertilizer.

The experimental effect is as follows: through the determination of a pot experiment, the result shows that the prevention effect of 50kg/667 square meter treatment on the tomato root knot nematode disease is the best, the prevention effect of the insect after 70 days is 88.41%, the prevention effect of the root knot nematode disease is 93.28%, the prevention effect of the root knot nematode disease is 92.37%, and the average plant height is 108.38 cm.

The technical principle is as follows:

the microbial preparation, the nutritional preparation and the performance modifier are combined to achieve the effect of preventing and controlling root knot nematode disease, clubroot disease, soil insect microbe and selenium.

Taking a microbial preparation of purple lilac spore bacteria, bacillus subtilis, trichoderma harzianum and abamectin as a bactericide; taking total matrines as pesticide; selenium-enriched yeast is used as a selenium fertilizer, wherein the yeast is used as an enzyme preparation; sugar alcohol and boron are compounded to be used as a trace element fertilizer preparation; calcium fulvate is used as a humus additive; amino acid is used as a nutritional agent; taking the tea seed cake as an organic fertilizer and a pesticide; as an energy supplement; grass peat is used as a dispersing agent, and a mixture rich in selenium and boron is formed through biological fermentation, so that the compound is effective in preventing and treating root knot nematode diseases, clubroot diseases and underground pests.

The experimental results are as follows:

tomato is used as an experimental example, common selenium-rich fertilizer is used as a reference, 1 kg of the selenium-rich fertilizer is applied to each square meter to be used as a test material, after harvesting, the root system is pulled out, the incidence of disease is observed, the reference incidence of disease is 29%, the experimental incidence of disease is 1%, and the incidence of disease is reduced by 28% when the selenium-rich fertilizer is applied; meanwhile, the selenium-rich pesticide fertilizer is basically free of insect pests, and the selenium-rich pesticide fertilizer can be completely used as a plant cultivation pesticide fertilizer.

Tests show that the disease and pest control rates of underground pests are respectively 90%, 93% and 88% when the selenium-rich ecological pesticide fertilizer is used for treating clubroot, root knot nematode and soil insects, the control is 62%, 69% and 85%, the acre yields of cucumber, Chinese cabbage, tomato and lettuce are respectively 8360 kg, 4266 kg, 14267 kg and 1860 kg when the selenium-rich ecological pesticide fertilizer is used, and the acre yields of conventional pesticide fertilizers are respectively 6825 kg, 3627 kg, 11625 kg and 1385 kg, so that the pest and disease treatment rate is obviously improved and the yield of the obtained vegetables is also obviously improved when the selenium-rich ecological pesticide fertilizer is used compared with the conventional pesticide fertilizer.

The selenium-rich pesticide fertilizer can be used in fields, gardens and trees.

The method is characterized in that:

1. the pesticide fertilizer disclosed by the invention is a broad-spectrum functional fertilizer, is applicable to all crops and any soil and climate conditions, and has the outstanding effects of high disease and insect damage resistance, increase in yield, improvement on quality, selenium enrichment, greenness, environmental friendliness and the like.

2. The fertilizer has no chemical residue and pesticide pollution, the unique effect of selenium-rich ferment microorganism fermentation organic matter nutrient combined with free amino acid can improve the utilization rate of N, P, K to about 70 percent, the prevention and treatment of root knot nematode disease and clubroot disease are realized by utilizing the parasitism of lilac violet spore fungus and trichoderma harzianum, the effective components can germinate to generate invasion nematode eggs and adult hyphae, and the components in the nematode body are absorbed to propagate in the nematode body to destroy the normal physiological metabolism of the nematode, and finally, the nematode eggs and adult are killed.

3. Compared with other organic fertilizers, the medical fertilizer reduces a large amount of field cultivation, saves manpower and material resources, and is rich in organic selenium to form a complete selenium-rich microbial medical fertilizer for preventing, treating and killing diseases and insects.

4. The medical fertilizer avoids soil hardening caused by long-term single use of inorganic fertilizer, has large residual quantity and can not meet the requirements of functional foods by using organic fertilizer, thereby improving the adaptability, functionality and content of effective nutrient components of agricultural products.

Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.

Claims (1)

1. A microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode diseases, clubroot diseases and underground pests is characterized by comprising the following components in parts by weight:

0.5-2 parts of purple lilac spore bacteria, 0.2-1.5 parts of bacillus subtilis, 0.1-1 part of trichoderma harzianum, 0.5-1.5 parts of total matrines, 0.5-2 parts of abamectin, 0.2-1 part of selenium-enriched yeast, 0.3-1.2 parts of sugar alcohol composite boron, 15-24 parts of calcium fulvate, 12-20 parts of amino acid, 18-28 parts of tea seed cake, 3-8 parts of sucrose and 10-50 parts of turf;

mixing tea seed cake and turf with 50% water content and selenium-enriched yeast and sucrose with 10% water content; stacking and carrying out aerobic fermentation to form a mixture A;

turning the mixture A, crushing, sequentially adding sugar alcohol composite boron, total matrines, amino acid raw powder and calcium fulvate, and fully and uniformly stirring to form a mixture B;

and adding the bacillus subtilis, the purple lilac spore bacteria, the trichoderma harzianum and the abamectin into the mixture B, and fully and uniformly stirring to obtain the selenium-rich pesticide fertilizer.

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