Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides an isolated lung storage perfusion device which can effectively clean and perfuse isolated lung in the transportation process, effectively remove metabolites generated in the transportation process of isolated lung, provide respiratory circulation of isolated lung in the transportation process, ensure the function of isolated lung transportation, and is safe and reliable.
According to the technical scheme provided by the invention, the isolated lung storage and perfusion device comprises a storage and perfusion box body which can be used for storing and transporting isolated lung, wherein a perfusion circulation mechanism which can be connected with the isolated lung in an adaptive manner and a respiratory circulation mechanism which can be connected with the isolated lung in an adaptive manner are arranged in the storage and perfusion box body, and the perfusion circulation mechanism and the respiratory circulation mechanism are electrically connected with a perfusion respiratory controller in the storage and perfusion box body, and the perfusion circulation mechanism and the respiratory circulation mechanism can be controlled to be in corresponding working states through the perfusion respiratory controller;
After the perfusion circulation mechanism is adaptively connected with pulmonary artery and pulmonary vein of the isolated lung, the perfusion respiration controller controls the perfusion circulation mechanism to perform continuous perfusion of the perfusion liquid on the connected isolated lung, and can remove metabolites generated by the isolated lung in the perfusion process of the perfusion liquid, and the perfusion respiration controller controls the respiration circulation mechanism to assist in respiration of the isolated lung so that the isolated lung can maintain a normal respiration state.
The storage and perfusion box body is internally provided with a heat insulation layer, and a storage and transportation refrigeration source which can provide low-temperature environment required by in-vitro lung storage and transportation is also arranged in the storage and perfusion box body.
The storage and transportation refrigeration source comprises a semiconductor refrigerator which is placed in a storage and transportation box body and can realize working refrigeration, the semiconductor refrigerator is connected with a perfusion respiration controller which controls the working state of the semiconductor refrigerator, the perfusion respiration controller can collect and store the temperature in the storage and transportation box body in real time through a temperature sensor, and the perfusion respiration controller can enable the storage and perfusion box body to achieve a temperature environment for in-vitro pulmonary transportation through the temperature sensor and the semiconductor refrigerator.
The perfusion circulation mechanism comprises a perfusion device capable of containing perfusion liquid, a perfusion driver matched with the perfusion device, and a pulmonary vein first connecting pipe, a pulmonary vein second connecting pipe, a pulmonary artery first connecting pipe and a pulmonary artery second connecting pipe which are capable of being communicated with the perfusion device;
The first connecting tube of the pulmonary vein and the second connecting tube of the pulmonary vein can be connected with the pulmonary vein in an adapting way, the first connecting tube of the pulmonary artery and the second connecting tube of the pulmonary artery can be connected with the pulmonary artery in an adapting way, the perfusion fluid in the perfusion fluid device can be driven by the perfusion driver to enter the pulmonary artery of the isolated lung through the first connecting tube of the pulmonary artery and the second connecting tube of the pulmonary artery, and the perfusion fluid entering the isolated lung can return to the perfusion fluid device again through the first connecting tube of the pulmonary vein and the second connecting tube of the pulmonary vein, so that continuous perfusion fluid perfusion circulation of the isolated lung can be realized.
The device also comprises a perfusion liquid outlet pipe and a perfusion liquid inlet pipe, wherein the perfusion liquid outlet pipe and the perfusion liquid inlet pipe can be adaptively connected with the perfusion liquid device, one end of the perfusion liquid inlet pipe is connected with the perfusion liquid device, the other end of the perfusion liquid inlet pipe is connected and communicated with the first pulmonary vein connecting pipe and the second pulmonary vein connecting pipe, one end of the perfusion liquid outlet pipe is connected with the perfusion liquid device, and the other end of the perfusion liquid outlet pipe is connected and communicated with the first pulmonary artery connecting pipe and the second pulmonary artery connecting pipe;
The perfusion liquid outlet pipe is provided with a filtering remover for removing metabolites in the isolated lung, and the perfusion liquid inlet pipe is provided with a gas separator for discharging gas in the perfusion liquid.
A liquid outlet one-way valve is arranged in the end part of the pouring liquid outlet pipe adjacent to the pouring liquid device, and a liquid inlet one-way valve is arranged in the end part of the pouring liquid inlet pipe adjacent to the pouring liquid device;
The perfusion device comprises a perfusion liquid container capable of containing perfusion liquid and a perfusion driving elastic ring arranged at the upper part in the perfusion liquid container, wherein the perfusion driving elastic ring is connected with the perfusion liquid container in an adaptive manner, the perfusion driving elastic ring can be driven to reciprocate in the perfusion liquid container through a perfusion driver, and when the perfusion driving elastic ring reciprocates in the perfusion liquid container, the perfusion liquid in the perfusion liquid inlet pipe can enter a perfusion liquid containing cavity at the lower part of the perfusion liquid container, and the perfusion liquid in the perfusion liquid containing cavity can enter a perfusion liquid outlet pipe.
The gas separator comprises a plurality of exhaust holes arranged on the perfusion liquid inlet pipe and a water-blocking breathable film arranged in the exhaust holes, and the filtering remover comprises an adsorption filtering film body capable of adsorbing and separating metabolic products.
The first connecting pipe clamp is arranged on the first connecting pipe of the pulmonary vein, the second connecting pipe clamp is arranged on the second connecting pipe of the pulmonary vein, the third connecting pipe clamp is arranged on the first connecting pipe of the pulmonary artery, and the fourth connecting pipe clamp is arranged on the second connecting pipe of the pulmonary artery.
The respiratory cycle mechanism comprises a gas generator capable of extracting and storing gas in the perfusion box body, an extraction driver capable of driving the gas generator and a gas filter capable of filtering the gas extracted by the gas generator, wherein a gas outlet of the gas filter is provided with a gas pipe matched with a gas pipe of an isolated lung, the extraction driver is electrically connected with a perfusion respiratory controller, and the perfusion respiratory controller can control the extraction driver to drive the gas generator to enter a required working state.
The gas generator comprises a generator shell, a gas extraction elastic ring arranged in the generator shell and a filtering air inlet arranged at the bottom of the generator shell, wherein the extraction driver can drive the gas extraction elastic ring to reciprocate in the generator shell, when the gas extraction elastic ring reciprocates in the generator shell, gas in the storage perfusion box body can enter the generator shell through the filtering air inlet, and the gas in the generator shell can enter an air pipe of an isolated lung through a gas filter and an air pipe.
The isolated lung storage and transportation device has the advantages that the isolated lung storage and transportation can be realized through the storage and transportation box, continuous perfusion of the isolated lung in the storage and transportation process can be realized through the perfusion circulation mechanism in the storage and transportation box, respiratory support of the isolated lung in the storage and transportation process can be realized through the respiratory circulation mechanism, the perfusion cleaning of the isolated lung in the transportation process can be effectively realized, the metabolic products generated in the transportation process of the isolated lung can be effectively removed, the respiratory circulation of the isolated lung in the transportation process can be provided, the function of the isolated lung in the transportation process can be ensured, and the device is safe and reliable.
Detailed Description
The invention will be further described with reference to the following specific drawings and examples.
In order to realize the perfusion cleaning of the isolated lung in the transfer process and effectively remove the metabolites generated in the transfer process of the isolated lung and provide the respiratory cycle of the isolated lung in the transfer process and ensure the transfer function of the isolated lung, the invention comprises a storage perfusion box body 1 which can be used for storing and transferring the isolated lung, a perfusion circulation mechanism 5 which can be adaptively connected with the isolated lung and a respiratory circulation mechanism 6 which can be adaptively connected with the isolated lung are arranged in the storage perfusion box body 1, wherein the perfusion circulation mechanism 5 and the respiratory circulation mechanism 6 are electrically connected with a perfusion respiratory controller in the storage perfusion box body 1, and the corresponding working states of the perfusion circulation mechanism 5 and the respiratory circulation mechanism 6 can be controlled through the perfusion respiratory controller;
After the perfusion circulation mechanism 5 is adaptively connected with the pulmonary artery and the pulmonary vein of the isolated lung, the perfusion respiratory controller controls the perfusion circulation mechanism 5 to perform continuous perfusion of the connected isolated lung with the perfusate, and can remove metabolites generated by the isolated lung in the perfusion process of the perfusate, and the perfusion respiratory controller controls the respiratory circulation mechanism 6 to assist the isolated lung to breathe so that the isolated lung can maintain a normal breathing state.
Specifically, the storage and perfusion box 1 is made of a material meeting medical standards, and specific material types can be selected according to needs, which are well known to those skilled in the art, and are not described herein. The storage and the transfer of the isolated lung can be realized through the storage and perfusion box body 1. During specific implementation, the storage and perfusion box body 1 is internally provided with a perfusion circulation mechanism 5, a breathing circulation mechanism 6 and a perfusion breathing controller, the perfusion circulation mechanism 5 and the breathing circulation mechanism 6 can be connected and matched with the isolated lung in the storage and perfusion box body 1, and the perfusion breathing controller can be electrically connected with the perfusion circulation mechanism 5 and the breathing circulation mechanism 6, namely, the perfusion breathing controller can control the corresponding working states of the perfusion circulation mechanism 5 and the breathing circulation mechanism 6.
During implementation, the perfusion circulation mechanism 5 can be connected with pulmonary artery and pulmonary vein of the isolated lung in an adaptive manner, continuous perfusion liquid perfusion of the connected isolated lung can be realized through the perfusion circulation mechanism 5, and in the perfusion process, metabolites generated by the isolated lung can be removed, and meanwhile, gas in the perfusion liquid can be separated and discharged, so that the reaction of the isolated lung after transplantation can be effectively reduced, and the reliability of the isolated lung after transplantation is ensured. The respiratory circulation mechanism 6 can assist the respiratory state of the isolated lung in the transportation process, so that the function of the isolated lung in the transportation process is ensured.
Specifically, the perfusion respiratory controller may be in a conventional micro-processing form, such as a single-chip microcomputer, and may be specifically selected according to needs, which is not described herein. Generally, in order to adapt to the storage of the isolated lung, the storage and perfusion box 1 comprises a first box cover 7 and a second box cover 11 matched with the first box cover 7, when the first box cover 7 and the second box cover 11 are mutually close to each other, the storage and perfusion box 1 can be formed, so that the isolated lung can be stored in a closed mode, and when the first box cover 7 and the second box cover 11 are mutually separated, the isolated lung can be placed in the storage and perfusion box 1. The first box cover 7 and/or the second box cover 11 are provided with the box pull ring 3, and the storage and pouring box 1 can be conveniently held by the box pull ring 3.
In the embodiment of the invention, the perfusion circulation mechanism 5 is arranged in the perfusion circulation placement area 8 in the first box cover 7, the breathing circulation mechanism 6 is arranged in the breathing circulation placement area 12 in the second box cover 11, and an in-vitro lung adaptive cavity can be formed between the first box cover 7 and the second box cover 11, so that when the in-vitro lung is arranged between the first box cover 7 and the second box cover 11, the perfusion circulation mechanism 5 and the breathing circulation mechanism 6 can be connected with the in-vitro lung in an adaptive manner, and continuous perfusion and auxiliary breathing support of the in-vitro lung are realized. The storage and perfusion box 1 is further provided with a box power supply 4 required by the work of the perfusion respiratory controller, the box power supply 4 can be in a lithium battery or other power supply modes, the box power supply 4 can be in a chargeable or replaceable mode, and the box power supply 4 and the perfusion respiratory controller can be in a common connection mode, which is well known to those skilled in the art, and is not described herein.
Further, a heat insulation layer 2 is arranged in the storage and perfusion box body 1, and a storage and transportation refrigeration source capable of providing low-temperature environment required by in-vitro lung storage and transportation is also arranged in the storage and perfusion box body 1. In the embodiment of the invention, the temperature condition required by the isolated lung in the storage and perfusion box 1 can be provided by storing and transferring the refrigeration source, so that the safety and reliability of the isolated lung in the storage and perfusion box 1 are ensured. Can reduce through insulating layer 2 and store the heat exchange of transportation refrigeration source in external world, ensure to store the stability of filling box 1 internal temperature, insulating layer 2 is in first box lid 7, second box lid 11, and insulating layer 2 can adopt the current form of commonly used, as long as can realize thermal-insulated purpose all, the specific case is not repeated.
The storage and transportation refrigeration source comprises an ice block placed in the storage and transportation box body 1 or a semiconductor refrigerator capable of realizing working refrigeration, the semiconductor refrigerator is connected with a perfusion respiration controller for controlling the working state of the semiconductor refrigerator, the perfusion respiration controller can collect and store the temperature in the storage and transportation box body 1 in real time through a temperature sensor, and the perfusion respiration controller can enable the storage and transportation box body 1 to achieve an in-vitro lung transportation temperature environment through the temperature sensor and the semiconductor refrigerator.
In the embodiment of the invention, when the ice blocks are arranged in the storage and perfusion box body 1, the temperature conditions in the storage and perfusion box body 1 can meet the temperature conditions required by the storage and transportation of the isolated lung through the low temperature energy of the ice blocks, and the refrigeration mode of the ice blocks is consistent with the refrigeration mode in the existing storage and transportation process of the isolated lung. Of course, in the concrete implementation, a semiconductor refrigerator can be adopted, the semiconductor refrigerator can be in a conventional common mode, the semiconductor refrigerator is electrically connected with the perfusion respiratory controller, and when the semiconductor refrigerator works, the temperature condition in the storage perfusion box body 1 can be kept at-5 ℃ to 10 ℃. Of course, other refrigeration forms can be adopted in the storage and perfusion box 1, so long as the temperature environment of the isolated lung in the storage and transportation process in the storage and perfusion box 1 can be satisfied, and no list is provided here.
As shown in fig. 4 and 5, the perfusion circulation mechanism 5 includes a perfusate container 23 capable of containing perfusate, a perfusion driver adapted to the perfusate container 23, and a pulmonary vein first connecting tube 15, a pulmonary vein second connecting tube 16, a pulmonary artery first connecting tube 29, and a pulmonary artery second connecting tube 30 capable of communicating with the perfusate container 23;
The first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16 can be connected with the pulmonary vein in an adapting way, the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30 can be connected with the pulmonary artery in an adapting way, the perfusion fluid in the perfusate device 23 can be driven by the perfusion driver to enter the pulmonary artery of the isolated lung through the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30, and the perfusion fluid entering the isolated lung can return to the perfusate device 23 again through the first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16, so that continuous perfusion fluid perfusion circulation of the isolated lung can be realized.
In the embodiment of the present invention, the perfusate container 23 is used to accommodate perfusate, and the perfusate can be placed in the perfusate container 23 by pre-filling or perfusion, and the perfusate is a liquid that can perfuse the isolated lung in the prior art, and the specific form of the perfusate is well known to those skilled in the art and will not be repeated here. The perfusion driver is matched with the perfusion fluid device 23, and can provide the power for the circulation of the perfusion fluid in the perfusion fluid device 23 to realize the perfusion circulation of the perfusion fluid. In order to be able to cooperate with the isolated lung, the first pulmonary vein connection 15 and the second pulmonary vein connection 16 are able to connect with the pulmonary vein of the isolated lung, and the first pulmonary artery connection 29 and the second pulmonary artery connection 30 are able to connect with the pulmonary artery of the isolated lung in an adapting way. After the device is connected with the isolated lung in an adapting way and the device 23 is driven by the perfusion driver, the perfusate in the device 23 can enter the pulmonary artery of the isolated lung through the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30, and the perfusate entering the isolated lung can return to the device 23 again through the first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16, and the circulating movement of the perfusate can be continuously realized along with the continuous cooperation of the perfusion driver and the device 23, so that the continuous perfusate perfusion circulation of the isolated lung can be realized.
Further, the device also comprises a perfusion liquid outlet pipe 26 and a perfusion liquid inlet pipe 19, wherein the perfusion liquid outlet pipe 26 can be adaptively connected with the perfusion liquid device 23, one end of the perfusion liquid inlet pipe 19 is connected with the perfusion liquid device 23, the other end of the perfusion liquid inlet pipe 19 is connected and communicated with the pulmonary vein first connecting pipe 15 and the pulmonary vein second connecting pipe 16, one end of the perfusion liquid outlet pipe 26 is connected with the perfusion liquid device 23, and the other end of the perfusion liquid outlet pipe 26 is connected and communicated with the pulmonary artery first connecting pipe 29 and the pulmonary artery second connecting pipe 30;
A filter remover 25 for removing metabolites in the isolated lung is arranged on the perfusion liquid outlet pipe 26, and a gas separator for discharging gas in the perfusion liquid is arranged on the perfusion liquid inlet pipe 19.
In the embodiment of the invention, the tube diameter of the infusion liquid inlet tube 19 is larger than the tube diameters of the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16, the length directions of the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16 are consistent with the length direction of the infusion liquid inlet tube 19, and the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16 can be communicated with the infusion liquid device 23 through the infusion liquid inlet tube 19. Similarly, the pipe diameter of the perfusion liquid outlet pipe 26 is larger than the pipe diameter of the first connecting pipe 29 of the pulmonary artery and the pipe diameter of the second connecting pipe 30 of the pulmonary artery, and the pipe diameters of the first connecting pipe 29 of the pulmonary artery and the second connecting pipe 30 of the pulmonary artery can be communicated with the perfusion liquid device 23 through the perfusion liquid outlet pipe 26.
A filter remover 25 is provided on the perfusion liquid outlet pipe 26 and a gas separator is provided on the perfusion liquid inlet pipe 19. The gas separator comprises a plurality of exhaust holes arranged on the pouring liquid inlet pipe 19 and a water-blocking air-permeable membrane 21 arranged in the exhaust holes, and the filter remover 25 comprises an adsorption filtering membrane 36 capable of adsorbing and separating metabolic products. Specifically, the gas separator comprises a gas separation container 20 which can be communicated with a perfusion liquid inlet pipe 19, a plurality of exhaust holes are formed in the gas separation container 20, a water-blocking air-permeable membrane 21 is arranged in each exhaust hole, perfusion liquid entering a perfusion liquid device 23 through the perfusion liquid inlet pipe 19 firstly enters the gas separation container 20, when gas exists in the perfusion liquid, the gas in the perfusion liquid can be discharged through the water-blocking air-permeable membrane 21, the perfusion liquid can be prevented from being discharged from the exhaust holes along with the gas through the water-blocking air-permeable membrane 21, after the gas is discharged, the perfusion liquid enters the perfusion liquid device 23 again, and the gas can be effectively discharged along with the continuous proceeding of the perfusion, so that the problem of air embolism caused by the existence of the gas in the perfusion liquid is avoided. In general, a liquid injection port 31 may be provided at the bottom of the gas separation vessel 20, and a desired liquid medicine, such as physiological saline, perfusion liquid, etc., may be injected into the gas separation vessel 20 through the liquid injection port 31, and the injected liquid may be mixed with the perfusion liquid in the gas separation vessel 20 and follow the perfusion cycle of the perfusion liquid.
In the embodiment of the invention, the filter remover 25 further comprises a filter removing container 35, the adsorption filtering membrane 36 is arranged in the filter removing container 35, the perfusate entering the pulmonary artery from the perfusate container 23 can firstly enter the filter removing container 35, after the perfusate fully contacts with the adsorption filtering membrane 36 in the filter removing container 35, the perfusate enters the pulmonary artery through the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30, the metabolite generated by the isolated lung can be adsorbed and removed through the adsorption filtering membrane 36, the adsorption filtering membrane 36 can be transversely arranged in the filter removing container 35, at this time, the perfusate in the perfusate container 23 firstly enters the lower part of the adsorption filtering membrane 36, and then flows out of the filter removing container 35 through the upper part of the filter removing container 35 after passing through the adsorption filtering membrane 36, and of course, the adsorption filtering membrane 36 can also adopt other distribution forms as long as the perfusate can be fully contacted. The adsorption filtering membrane 36 can adopt the matching form of the conventional commonly used filter screen membrane and the medical adsorbent, the medical adsorbent can adopt medical carbon powder and the like, and the adsorption filtering membrane 36 can also adopt other structural forms, so long as the adsorption and the filtration of the metabolic products generated in the in-vitro lung perfusion process can be realized, and the details are not described.
Further, a liquid outlet one-way valve 24 is arranged in the end part of the pouring liquid outlet pipe 26 adjacent to the pouring liquid device 23, and a liquid inlet one-way valve 22 is arranged in the end part of the pouring liquid inlet pipe 19 adjacent to the pouring liquid device 23;
the perfusate container 23 includes a perfusate container 32 capable of containing perfusate and a perfusate driving elastic ring 33 disposed at the upper part in the perfusate container 32, the perfusate driving elastic ring 33 is adaptively connected with the perfusate container 32, the perfusate driving elastic ring 33 can be driven to reciprocate in the perfusate container 32 by a perfusate driver, and when the perfusate driving elastic ring 33 reciprocates in the perfusate container 32, the perfusate in the perfusate inlet pipe 19 can enter a perfusate containing cavity 34 at the lower part of the perfusate container 32, and the perfusate in the perfusate containing cavity 34 can enter the perfusate outlet pipe 26.
In the embodiment of the invention, the liquid outlet one-way valve 24 and the liquid inlet one-way valve 22 can adopt the conventional common forms, the one-way flow of the perfusate can be realized through the liquid inlet one-way valve 22 and the liquid outlet one-way valve 24, the liquid inlet one-way valve 22 is positioned between the gas separation container 20 and the perfusate container 23, the liquid outlet one-way valve 24 is positioned between the perfusate container 23 and the filtering removal container 35, and the reliability of the flow of the perfusate in the perfusion process can be ensured through the liquid inlet one-way valve 22 and the liquid outlet one-way valve 24.
The perfusate container 23 can accommodate perfusate through the perfusate container 32, the perfusion driving elastic ring 33 is located at the upper part in the perfusate container 32, the perfusion driving elastic ring 33 can be made of elastic components such as springs, the length direction of the perfusion driving elastic ring 33 is consistent with the length direction of the perfusate container 32, the upper part of the perfusate container 32 can follow the perfusion driving elastic ring 33 to reciprocate, namely, the upper part of the perfusate container 32 can be made of an elastic film, thereby accommodating the perfusion driving elastic ring 33 in the perfusate container 32 and ensuring the reciprocation of the perfusion driving elastic ring 33 in the perfusate container 32, and the direction of the reciprocation of the perfusion driving elastic ring 33 is the length direction of the perfusion driving elastic ring 33. When the elastic ring 33 is driven by compression, negative pressure can be generated in the perfusate container 32, so that perfusate in the perfusate inlet pipe 19 can enter the perfusate containing cavity 34 at the lower part of the perfusate container 32 through the liquid inlet one-way valve 22, and when the elastic ring 33 is driven by compression and reset, perfusate in the perfusate containing cavity 34 can enter the perfusate outlet pipe 26 through the liquid outlet one-way valve 24.
When the above structure is adopted in the pouring liquid device 23, the pouring driver can drive the pouring driving elastic ring 33 to reciprocate, and in specific implementation, the pouring driver comprises a pouring elastic ring driving body 9 and a pouring elastic ring driving plate 10, the pouring elastic ring driving body 9 is arranged on the first box cover 7, the pouring elastic ring driving plate 10 is positioned right above the pouring driving elastic ring 33, the pouring elastic driving body 9 can adopt a linear motor, the pouring elastic ring driving plate 10 is adaptively connected with the pouring elastic driving body 9, and the pouring elastic driving body 9 can drive the pouring driving elastic ring 33 to reciprocate through the pouring elastic ring driving plate 10. Of course, the filling elastic driving body 9 can also adopt the matching form of a motor and a screw nut and the like, as long as the driving of the reciprocating motion of the priming driving elastic ring 33 can be achieved by the priming elastic ring driving plate 10, the selection may be specifically selected according to needs, and will not be described herein. The perfusion elastic driving body 9 is electrically connected with a perfusion breathing controller, and the perfusion breathing controller can control the working state of the perfusion elastic driving body 9, so that the driving control of the reciprocation of the perfusion driving elastic ring 33 can be realized.
Further, a first connecting tube clamp 17 is provided on the first pulmonary vein connecting tube 15, a second connecting tube clamp 18 is provided on the second pulmonary vein connecting tube 16, a third connecting tube clamp 27 is provided on the first pulmonary artery connecting tube 29, and a fourth connecting tube clamp 28 is provided on the second pulmonary artery connecting tube 30.
In the embodiment of the present invention, the state of the pulmonary vein first connecting tube 15 flowing the perfusate can be controlled by the first connecting tube clamp 17, and the first connecting tube clamp 17 can be an existing commonly used medical clamp, specifically can be selected according to the needs, and will not be described here again. Similarly, the second connecting tube clamp 18, the third connecting tube clamp 27 and the fourth connecting tube clamp 28 have the same specific functions as the first connecting tube clamp 17, and the description of the first connecting tube clamp 17 is specifically referred to.
Further, the respiratory cycle mechanism 6 includes a gas generator capable of extracting and storing the gas in the perfusion box 1, an extraction driver capable of driving the gas generator, and a gas filter 40 capable of filtering the gas extracted by the gas generator, a gas outlet of the gas filter 40 is provided with a gas tube 41 capable of adapting to a gas tube of the isolated lung, the extraction driver is electrically connected with the perfusion respiratory controller, and the perfusion respiratory controller can control the extraction driver to drive the gas generator into a required working state.
In the embodiment of the invention, the gas in the perfusion box 1 can be extracted and stored through the gas generator, the gas generator can be driven to work through the extraction driver, and the gas to be introduced into the trachea of the isolated lung can be filtered through the gas filter 40, so that impurities and the like in the gas are prevented from entering the trachea of the isolated lung. The tracheal tube 41 is adapted to the ex-vivo lung, the tracheal tube 41 having a tracheal insertion end 42, i.e. the tracheal tube 41 can be inserted into the trachea of the ex-vivo lung via the tracheal insertion end 42. The extraction driver is electrically connected with the perfusion respiratory controller, and the perfusion respiratory controller controls the extraction driver to work, so that the extraction driver can be matched with the gas generator, gas in the storage perfusion box body 1 is extracted and then is sent into the gas pipe of the isolated lung, the respiration of the isolated lung is assisted, and the function of the isolated lung in the operation process is maintained. Generally, the frequency of the gas sent into the trachea of the isolated lung by the extraction driver and the gas generator is generally lower than the normal respiratory frequency of the isolated lung, and the gas is directly discharged into the storage and perfusion box 1 after the isolated lung breathes, so that the gas can be recycled.
As shown in fig. 6, the gas generator comprises a generator shell 37, a gas extraction elastic ring 38 arranged in the generator shell 37 and a filtering gas inlet 39 arranged at the bottom of the generator shell 37, wherein the extraction driver can drive the gas extraction elastic ring 38 to reciprocate in the generator shell 37, when the gas extraction elastic ring 38 reciprocates in the generator shell 37, the gas stored in the perfusion box 1 can enter the generator shell 37 through the filtering gas inlet 39, and the gas in the generator shell 37 can enter the gas pipe of the isolated lung through a gas filter 40 and a gas pipe 41.
In the embodiment of the invention, the gas generator can adopt the form of the existing common pedal inflator, specifically, the gas generator comprises a generator shell 37, a gas extraction elastic ring 38 and a filtering air inlet 39, wherein the gas extraction elastic ring 38 is arranged in the generator shell, the filtering air inlet 39 is an air inlet provided with a gas filtering membrane, the gas extraction elastic ring 38 can reciprocate in the generator shell 37, the length direction of the gas extraction elastic ring 38 is consistent with the length direction of the generator shell 37, the generator shell 37 can be generally made of a flexible membrane, so that the gas extraction elastic ring 38 can be accommodated in the generator shell 37, and the reciprocating movement following the gas extraction elastic ring 38 can be realized, and the gas extraction elastic ring 38 can be generally made of a spring ring. When the gas extraction elastic ring 38 is compressed in the generator shell 37, negative pressure can be generated in the generator shell 37, gas stored in the pouring box 1 can enter the generator shell 37 through the filtering air inlet 39, and when the gas extraction elastic ring 38 is reset, the gas in the generator shell 37 can enter the gas filter 40. The gas filter 40 may take the form of a conventional gas filter, such as a carbon dioxide absorbent.
After the gas is fully filtered by the gas filter 40, the gas enters the trachea of the isolated lung through the trachea cannula 41, so that the cleanliness of the gas entering the trachea of the isolated lung is ensured. Generally, the tube diameter of the tracheal tube 41 may be smaller than that of the isolated lung, so that after the isolated lung breathes, the breathed gas or the gas not breathed can be directly discharged into the storage and perfusion box 1 through the gap between the tracheal tube 41 and the isolated lung. In specific implementation, the air tube 41 may be further provided with an exhaust valve 43, where the exhaust valve 43 may be a common air overflow valve, that is, when the gas in the generator housing 37 is more, the exhaust valve 43 can exhaust redundant gas, or the exhaust valve 43 can exhaust the gas in the isolated lung airway through the exhaust valve 43, so as to realize support of the isolated lung respiration process.
When the gas generator adopts the above structural form, the extraction driver comprises an extraction elastic ring driving body 13 and an extraction elastic ring driving plate 14, the extraction elastic ring driving body 13 can adopt a linear motor and other forms, the extraction elastic ring driving plate 14 is connected with the output end of the extraction elastic ring driving body 13, the extraction elastic ring driving body 13 and the extraction elastic ring driving plate 14 are matched to realize the reciprocating motion of driving the gas extraction elastic ring 38, the extraction elastic ring driving body 13 is installed in the second box cover 11, and the extraction elastic ring driving plate 14 is positioned right above the gas extraction elastic ring 38. The specific form and driving process of the pumping elastic ring driving body 13 and the pumping elastic ring driving plate 14 in cooperation with the driving gas pumping elastic ring 38 are similar to those of the pumping elastic ring driving body 9 and the pumping elastic ring driving plate 10 for driving the pumping driving elastic ring 33, and specific reference may be made to the above description, and details are not repeated here.