CN110632288A - Specific protein analyzer and testing method based on same - Google Patents
Specific protein analyzer and testing method based on sameDownload PDFInfo
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- CN110632288A CN110632288ACN201910807096.5ACN201910807096ACN110632288ACN 110632288 ACN110632288 ACN 110632288ACN 201910807096 ACN201910807096 ACN 201910807096ACN 110632288 ACN110632288 ACN 110632288A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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Abstract
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the field of medical instruments, in particular to a specific protein analyzer and a testing method based on the specific protein analyzer.
[ background of the invention ]
At present, a specific protein analyzer is used as a mainstream instrument for analyzing the content of specific protein in human body fluid, and is widely applied. The specific protein analyzer utilizes the scattering turbidimetric principle to detect immune complex particles formed by the reaction of antigen and antibody suspended in buffer. When laser passes through, the change intensity of the scattered light velocity generated by suspended particles is in direct proportion to the antigen concentration, when the reaction reaches the highest peak, the amount of the formed product is measured, the height of the velocity peak value is in direct proportion to the antigen content, and the velocity peak value is converted into the measured antigen concentration through processing.
CRP and SAA are the important indexes recognized in the medical field for identifying bacterial infection or viral infection at present, and the ratio SAA/CRP has clinical guidance significance. The existing instrument for measuring the specific protein can only manually sample, and is inconvenient to operate.
[ summary of the invention ]
In order to solve the technical problems, embodiments of the present invention provide a specific protein analyzer capable of automatic sample injection and a test method based on the specific protein analyzer.
In order to solve the above technical problem, an embodiment of the present invention provides the following technical solutions:
in one aspect, there is provided a specific protein analyzer comprising: the host comprises a shell, and a reaction module, a sampling assembly, a liquid adding assembly, a liquid path system and a control board card which are arranged in the shell, wherein the control board card is used for controlling the reaction module, the sampling assembly, the liquid adding assembly and the liquid path system, the reaction module is used for providing a reaction site of an antigen antibody, the liquid adding assembly is used for adding a reagent into the reaction module, the sampling assembly is used for sucking and spitting a sample and/or an antibody, the liquid path system is connected with the liquid adding assembly and the sampling assembly, and the liquid path system is used for transporting the reagent, the sample and/or the antibody; and the automatic sample injector, the automatic sample injector includes mixing subassembly, autoinjection subassembly and refrigeration storehouse subassembly, the autoinjection subassembly includes test-tube rack sample introduction district, test-tube rack feeding district and test-tube rack uninstallation district, the autoinjection subassembly be used for with place in the test-tube rack in test-tube rack sample introduction district transports in proper order extremely the test-tube rack feeding district reaches the test-tube rack uninstallation district, the test-tube rack is including the test tube that carries the sample that awaits measuring, the mixing subassembly is used for the mixing the test tube, refrigeration storehouse subassembly is provided with two antibody bottle and places the position.
In some embodiments, the reaction module comprises a module base, a laser, a diaphragm, a photoelectric conversion plate, a shielding case and a reaction cup set; the laser, the diaphragm, the reaction cup group, the photoelectric conversion plate and the shielding case are all arranged on the module base and are sequentially arranged along the optical axis of the laser; the laser is used for emitting laser to the reaction cup group through the diaphragm; the diaphragm is used for filtering laser emitted by the laser; the reaction cup group is used for providing a reaction site of antigen and antibody; the photoelectric conversion plate is used for receiving scattered light of the reaction cup group and converting the scattered light into an electric signal; the liquid adding assembly is used for adding reagents into the reaction cup group.
In some embodiments, the reaction module further comprises a stirring motor and a magnet, wherein the magnet is fixedly arranged on a rotating shaft of the stirring motor; the reaction cup group comprises reaction cups and heating blocks; the reaction cup is used for providing a reaction site, a magnetic bar stirrer is arranged in the reaction cup, and when the stirring motor drives the magnet to rotate, the magnetic bar stirrer rotates together; the outer surface of the heating block is covered with a heating film for providing a temperature required for the reaction of the reaction site.
In some embodiments, the sampling assembly comprises a sampling holder, a piercing support, a feed drive, a piercing drive, a sampling needle, and a swab; the puncture bracket is movably arranged on the sampling fixing seat, the sampling needle is movably arranged on the puncture bracket, and the swab is fixedly arranged on the puncture bracket; the feeding driving device is used for driving the puncture bracket to move in the horizontal direction; the puncture driving device is used for driving the sampling needle to move along the vertical direction; the swab is used to clean the sampling needle as it moves.
In some embodiments, the liquid adding assembly comprises a liquid adding bottom plate, a liquid adding pipe seat, a liquid adding pipe and a liquid adding driving device; the liquid adding pipe seat is movably arranged on the liquid adding bottom plate, and the liquid adding pipe is fixedly arranged on the liquid adding pipe seat; the liquid feeding driving device is used for driving the liquid feeding pipe seat to move along the feeding direction, so that the liquid feeding pipe is used for adding reagents to the reaction module.
In some embodiments, the blending assembly comprises a first linear drive, a second linear drive, a rotary drive, and a jaw; the first linear driving device is used for driving the clamping jaw to move along the horizontal direction; the second linear driving device is used for driving the clamping jaw to move along the vertical direction; the rotary driving device is used for driving the clamping jaws to rotate; the clamping jaw is used for clamping the test tube.
In some embodiments, the first linear driving device comprises a first lead screw mechanism and a first lead screw driving motor, and the first lead screw driving motor is used for driving the second linear driving device to move along the horizontal direction through the first lead screw mechanism; the second linear driving device comprises a second screw rod mechanism and a second screw rod driving motor, and the second screw rod driving motor is used for driving the rotary driving device to move along the vertical direction through the second screw rod mechanism; the rotary driving device comprises a belt wheel mechanism and a belt wheel driving motor, and the belt wheel driving motor is used for driving the clamping jaw to rotate through the belt wheel mechanism.
In some embodiments, the automated sample assembly further comprises a base plate, a sample assembly, a back plate assembly, a lateral sample assembly, an unload assembly, and a scanner assembly; the sample feeding assembly comprises a first linear slide rail, a sample feeding pusher dog, a first motor and a first belt pulley assembly, the first linear slide rail is used for guiding the test tube rack to be transported from the sample feeding area of the test tube rack to the feeding area of the test tube rack, the sample feeding pusher dog is used for driving the test tube rack to be transported from the sample feeding area of the test tube rack to the feeding area of the test tube rack, and the first motor is used for driving the sample feeding pusher dog through the first belt pulley assembly; the rear back plate assembly comprises a rear back plate and a counter arranged on the rear back plate, and the counter is used for counting the test tube positions of the test tube rack; the transverse sampling assembly comprises a transverse sampling bottom plate, and a second motor, a second linear guide rail, a second belt wheel assembly and a transverse shifting claw assembly which are arranged on the transverse sampling bottom plate, wherein the second linear guide rail is used for guiding the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, the transverse sampling shifting claw is used for driving the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, and the second motor is used for driving the transverse sampling shifting claw through the second belt wheel assembly; the unloading assembly comprises an unloading support, and a third motor, a third belt wheel assembly, a third linear guide rail and an unloading push plate which are arranged on the unloading support, wherein the third linear guide rail is used for guiding the test tube rack to be unloaded from the test tube unloading area, the unloading push plate is used for driving the test tube rack to be unloaded from the test tube unloading area, and the third motor is used for driving the unloading push plate through the third belt wheel assembly; the scanner component is used for scanning a bar code attached to the test tube; the refrigerating bin body is used for refrigerating through the cold end of the Peltier semiconductor, the hot end of the Peltier semiconductor is installed on the radiator, and the radiator radiates heat through the fan.
In some embodiments, the autosampler is provided with a key switch for controlling the sampling assembly to aspirate sample and/or antibody.
In another aspect, there is provided a test method based on a specific protein analyzer as described above, the method comprising: the sampling assembly is used for diluting the sample in the test tube and spitting the diluted sample and the antibody to the reaction cup group; and after the preset time, the reaction module acquires data.
In some embodiments, the sampling assembly dilutes the sample and spits the diluted sample and the antibody to the reaction cup set, and specifically includes: the sampling component sucks a sample in the test tube; the sampling assembly moves to a pre-dilution position and spits a sample; the sampling component moves to the antibody position to absorb the antibody; the sampling assembly returns to a pre-dilution position to absorb the diluted sample; the sampling assembly moves to the reaction cup group to spit out the sample and the antibody; stirring the antigen and antibody in the reaction cup group.
Compared with the prior art, in the specific protein analyzer and the testing method based on the specific protein analyzer provided by the embodiment of the invention, the automatic sample injector is configured, and the test tube rack placed in the sample feeding area of the test tube rack is transported to the sample feeding area of the test tube rack by the automatic sample injector, so that the specific protein analyzer capable of automatically injecting samples is realized.
[ description of the drawings ]
One or more embodiments are illustrated in drawings corresponding to, and not limiting to, the embodiments, in which elements having the same reference number designation may be represented as similar elements, unless specifically noted, the drawings in the figures are not to scale.
FIG. 1 is a perspective view of a specific protein analyzer according to one embodiment of the present invention;
FIG. 2 is a disassembled schematic view of the specific protein analyzer shown in FIG. 1;
FIG. 3 is a disassembled schematic view of the specific protein analyzer shown in FIG. 1 at another angle;
FIG. 4 is a cross-sectional view of the reaction module of the specific protein analyzer shown in FIG. 1;
FIG. 5 is a perspective view of a sampling assembly of the particular protein analyzer shown in FIG. 1;
FIG. 6 is a perspective view of a liquid addition assembly of the particular protein analyzer shown in FIG. 1;
FIG. 7 is a disassembled schematic view of an autosampler assembly of the specific protein analyzer shown in FIG. 1;
FIG. 8 is a perspective view of a homogenizing assembly of the particular protein analyzer shown in FIG. 1;
FIG. 9 is a flow chart of a method for testing based on the specific protein analyzer shown in FIG. 1 according to another embodiment of the present invention;
fig. 10 is a detailed flowchart of step S1 in fig. 9.
[ detailed description ] embodiments
In order to facilitate an understanding of the invention, the invention is described in more detail below with reference to the accompanying drawings and detailed description. It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may be present. The terms "vertical," "horizontal," "left," "right," "inner," "outer," and the like as used herein are for descriptive purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1, an analyzer for specific proteins according to an embodiment of the present invention includes afront case 1, amain frame 2 and an auto-sampler 3. The front side of thefront shell 1 is provided with adisplay screen 4 for man-machine interaction and displaying a test result. Thehost machine 2 is provided with athermal printer 5, and an output port of thethermal printer 5 is arranged at the top of thehost machine 2 and used for printing and outputting a test result. Theautosampler 3 is provided with akey switch 6 for manual sample injection mode as well as emergency mode. Theautosampler 3 comprises a refrigeration bin assembly which is provided with two antibody bottle placement positions, so that the specific protein analyzer can support both single CRP and SAA project tests and CRP and SAA joint inspection. The refrigerating bin assembly is used for refrigerating through the cold end of the Peltier semiconductor, the hot end of the Peltier semiconductor is installed on the radiator, and the radiator radiates heat through the fan.
Referring to fig. 2 and 3, themain body 2 includes a housing, and areaction module 21, asampling assembly 22, aliquid feeding assembly 23, a liquid path system and a control card 24 mounted in the housing. Thebottom plate 25 of the housing is provided with a first mounting plate and a second mounting plate, which are opposite to each other and are both vertical on the bottom plate. Thereaction module 21 is mounted on thebase plate 25 for providing a reaction site for antigen and antibody. Thesampling assembly 22 and theliquid adding assembly 23 are both mounted on the first mounting plate, thesampling assembly 22 is used for sucking and spitting a sample and/or an antibody, and theliquid adding assembly 23 is used for adding a reagent in thereaction module 21. The liquid path system is arranged between the first mounting plate and the second mounting plate and is connected with thesampling assembly 22 and theliquid adding assembly 23 for transporting reagents, samples and/or antibodies. The control clamping plate 24 is installed above the first installation plate and the second installation plate, connected with theautomatic sampler 3, thereaction module 21, thesampling assembly 22, theliquid adding assembly 23 and the liquid path system, and used for controlling theautomatic sampler 3, thereaction module 21, thesampling assembly 22, theliquid adding assembly 23 and the liquid path system to work.
Referring to fig. 4, thereaction module 21 includes amodule base 211, alaser 212, adiaphragm 213, aphotoelectric conversion plate 214, ashielding cover 215, a reaction cup set 216, a stirringmotor 217, and amagnet 218. Thelaser 212, thediaphragm 213, the reaction cup set 216, thephotoelectric conversion plate 214, and theshield cover 215 are all mounted on themodule base 211 and arranged in sequence along the optical axis O of thelaser 212. Alaser 212 is connected to the control card, thelaser 212 being arranged to emit laser light via adiaphragm 213 to a set ofreaction cups 216 to provide a source of light for testing. Thestop 213 is used to filter the laser light emitted from thelaser 212 to filter out stray light. Thereaction cup set 216 is used for providing a reaction site for antigen and antibody, and thereaction cup set 216 receives the laser light emitted from thelaser 212 and transmits the scattered light to thephotoelectric conversion panel 214. Thephotoelectric conversion plate 214 is connected with the control card plate, and thephotoelectric conversion plate 214 is used for receiving the scattered light transmitted by thereaction cup group 216 and converting an optical signal into an electrical signal so as to realize the scattering turbidimetry principle. Theshield case 215 provides a reference plane for thephotoelectric conversion panel 214 and shields an external electromagnetic signal. Stirringmotor 217 is connected with the control cardboard, andmagnet 218 fixed mounting is on stirringmotor 217's pivot, andreaction cup group 216 includes reaction cup and heating block. The reaction cup is used for providing a reaction site for antigen and antibody, receives laser emitted by thelaser 212 and transmits scattered light to thephotoelectric conversion plate 214, a magnetic rod stirrer is placed in the reaction cup, and when the stirringmotor 217 drives themagnet 218 to rotate, the magnetic rod stirrer rotates together, so that the antigen and antibody in the reaction cup are fully combined and reacted. The surface of the heating block is covered with a heating film for providing temperature conditions required for antigen-antibody reaction.
Referring to fig. 5, thesampling assembly 22 includes asampling holder 221, a piercingholder 222, afeeding driving device 223, a piercingdriving device 224, asampling needle 225 and aswab 226. Thepuncture bracket 222 is movably mounted on thesampling fixing seat 221, thesampling needle 225 is movably mounted on thepuncture bracket 222 and connected with the liquid path system, and theswab 226 is fixedly mounted on thepuncture bracket 222. Thefeeding driving device 224 is connected to the control card board, thefeeding driving device 224 is used for driving thepuncture holder 222 to move along the horizontal direction, so that thesampling needle 225 mounted on thepuncture holder 222 is positioned right above the test tube or the reaction cup group, and thefeeding driving device 224 can be realized by a transmission mode of driving a belt by a belt wheel. Thepuncture driving device 224 is connected with the control card board, and thepuncture driving device 224 is used for driving thesampling needle 225 to move along the vertical direction, so that the sampling needle positioned right above the test tube or the reaction cup group extends into the test tube or the reaction cup group, and thepuncture driving device 224 needs to drive thesampling needle 225 to complete the puncture action along the vertical direction, so that thepuncture driving device 224 can be realized by adopting a screw rod transmission mode. Thesampling needle 225 is used for sucking and spitting a sample and/or an antibody, and an infusion tube of a wiring harness and a liquid path system can be arranged between thesampling fixing seat 221 and thepuncture bracket 224 through a drag chain. Theswab 226 is used to clean thesampling needle 225 as it is moved. The aforementioned push-button switch can also control thesampling assembly 22 to aspirate and spit the sample and/or the antibody.
Referring to fig. 6, the fillingassembly 23 includes a fillingbottom plate 231, a fillingtube seat 232, a fillingtube 233 and afilling driving device 234. The liquidfeeding pipe seat 232 is movably installed on the liquid feedingbottom plate 231, and theliquid feeding pipe 233 is fixedly installed on the liquidfeeding pipe seat 232 and connected with the liquid path system. The liquidfeeding driving device 234 is connected with the control clamping plate, the liquidfeeding driving device 234 is used for driving the liquidfeeding pipe seat 232 to move along the horizontal direction, so that theliquid feeding pipe 233 is located above the reaction cup group, theliquid feeding pipe 233 can be used for adding reagents to the reaction cup group, and the liquidfeeding driving device 234 can be realized in a transmission mode that a belt wheel drives a belt because the load of the liquidfeeding driving device 234 is small.
Referring to fig. 7 and 8, theautosampler 3 further comprises a homogenizingassembly 31, anautosampler assembly 32, and the aforementionedrefrigeration bin assembly 33. Theautosampler assembly 32 includes arack sampling area 321, arack feed area 322, and arack unloading area 323. The automaticsample introduction assembly 32 is used for sequentially transmitting the test tube racks placed in the test tube racksample introduction area 321 to the test tuberack feeding area 322 and the test tuberack unloading area 323, and the test tube racks comprise test tubes loaded with samples to be tested. The mixingassembly 31 is used for mixing the test tubes. Theautosampler assembly 32 further includes a base plate, a sample introduction assembly, a back rest assembly, a lateral sample introduction assembly, an unloading assembly, and a scanner assembly. The sampling component comprises a first linear slide rail, a sampling pusher dog, a first motor and a first belt pulley component. The first slide rail is used for guiding the test-tube rack to be transported to the test-tube rack feeding area from the test-tube rack feeding area, the sample feeding pusher dog is used for driving the test-tube rack to be transported to the test-tube rack feeding area from the test-tube rack feeding area, and the first motor is used for driving the sample feeding pusher dog through the first belt pulley component. The back backup plate subassembly includes back backup plate and installs in the counter of back backup plate, and the counter links to each other with the control cardboard, and the counter is used for making statistics of the test tube position of test-tube rack, and every test tube position that feeds of test-tube rack, then the test tube position that the counter counted increases one to this calculates the position that the test-tube rack fed. The transverse sample injection assembly comprises a transverse sample injection bottom plate, and a second motor, a second linear guide rail, a second belt wheel assembly and a transverse shifting claw assembly which are arranged on the transverse sample injection bottom plate. The second linear guide is used for guiding the test-tube rack to be transported to the test-tube rack unloading area from the test-tube rack feeding area, the transverse sampling pusher dog is used for driving the test-tube rack to be transported to the test-tube rack unloading area from the test-tube rack feeding area, the second motor is connected with the control clamping plate, and the second motor is used for driving the transverse sampling pusher dog through the second belt wheel assembly. The unloading assembly comprises an unloading support, and a third motor, a third belt wheel assembly, a third linear guide rail and an unloading push plate which are arranged on the unloading support. The third linear guide is used for guiding the test-tube rack to unload from the test-tube rack unloading area, the unloading push plate is used for driving the test-tube rack to unload from the test-tube rack unloading area, the third motor is connected with the control clamping plate, and the third motor is used for driving the unloading push plate through the third belt wheel assembly. Scanner subassembly connection control cardboard, scanner subassembly are used for the bar code of pasting on the scanning test tube and establish to discernment test tube.
The kneadingassembly 31 includes a firstlinear drive 311, a secondlinear drive 312, arotary drive 313, and ajaw 314. A firstlinear drive 311 is connected to the control card, the firstlinear drive 311 being used to drive thejaws 314 in a horizontal direction so that thejaws 314 are positioned directly above the test tube. The firstlinear driving device 311 comprises a first lead screw mechanism and a first lead screw driving motor, the first lead screw driving motor is connected with the control card board, and the first lead screw driving motor is used for driving the secondlinear driving device 312 to move along the horizontal direction through the first lead screw mechanism. The secondlinear driving device 312 is connected with the control chuck plate, and the secondlinear driving device 312 is used for driving the clampingjaws 314 to move along the vertical direction, so that the clampingjaws 314 are close to the test tube rack, and then the clampingjaws 314 can clamp the test tube. The secondlinear driving device 312 includes a second lead screw mechanism and a second lead screw driving motor, the second lead screw driving motor is connected to the control card board, and the second lead screw driving motor is used for driving therotary driving device 313 to move along the vertical direction through the second lead screw mechanism. Therotary driving device 313 is connected with the control clamping plate, and therotary driving device 313 is used for driving the clampingjaws 314 to rotate so as to uniformly mix the test tubes clamped by the clampingjaws 314. Therotary driving device 313 comprises a belt wheel mechanism and a belt wheel driving motor, the belt wheel driving motor is connected with the control clamping plate, and the belt wheel driving motor is used for driving the clamping jaw to rotate through the belt wheel mechanism. The clampingjaws 314 are connected with the control clamping plate, and the clampingjaws 314 are used for clamping test tubes.
When the specific protein analyzer is used for automatic sample introduction, the following steps are carried out:
the test-tube rack is including the test tube that carries the sample that awaits measuring, presss from both sides the test-tube rack clamping in the test-tube rack sample introduction area, transports the test-tube rack to the test-tube rack feeding area from the test-tube rack sample introduction area through advancing the appearance subassembly. When the test tube position sign test-tube rack that the counter was makeed statistics of has got into the test-tube rack and fed the district, the bar code of establishing is pasted on the scanning test tube to the scanner subassembly to discernment test tube, this test tube is got and is put back the test-tube rack after the mixing to the mixing subassembly clamp, then, the sample subassembly is inhaled to this test tube and is told sample and/or antibody, after final sample subassembly had absorbed sample and antibody, transversely advance the appearance subassembly and transport the test-tube rack to the test-tube rack uninstallation district, the uninstallation subassembly will be located the test-tube rack uninstallation of test-tube.
The manual sample introduction process of the specific protein analyzer is as follows:
place the test tube under the sampling needle of sampling subassembly, ensure that the sampling needle inlet is below the sample liquid level, triggerkey switch 6, the sample and/or antibody are inhaled and spit to the sampling subassembly, and sample and antibody have been absorbed to the final sampling subassembly.
After the automatic sample introduction or the manual sample introduction of the specific protein analyzer is completed, the sampling assembly spits the absorbed sample and the antibody into the reaction cup, the liquid adding assembly adds the reagent into the reaction cup, after the stirring motor drives the magnetic bar stirrer in the reaction cup to stir, the laser emits laser to the reaction cup, the photoelectric conversion plate receives scattered light transmitted by the reaction cup, an electric signal containing a test result is generated and transmitted to the control card plate, the electric signal is displayed on the display screen, and the test process is completed at the moment.
Referring to fig. 9, another embodiment of the present invention provides a method for testing a specific protein analyzer based on the foregoing embodiments, the method comprising:
step S1: the sampling assembly spits the diluted sample and the antibody to the reaction cup group;
step S2: and after the preset time, the reaction module acquires data. The data is the test result, which includes CRP value, SAA value and SAA/CRP ratio.
Referring to fig. 10, in step S1, the method for spitting the diluted sample and the antibody to the reaction cup set by the sampling assembly specifically includes:
s11: the sampling component sucks a sample in the test tube;
s12: the sampling assembly moves to a pre-dilution position and spits a sample;
s13: the sampling component moves to the antibody position to absorb the antibody;
s14: the sampling assembly returns to a pre-dilution position to absorb the diluted sample;
s15: the sampling assembly moves to the reaction cup group to spit out the sample and the antibody;
s16: stirring the antigen and antibody in the reaction cup group.
Compared with the prior art, the specific protein analyzer and the test method based on the specific protein analyzer provided by the embodiment of the invention have the advantages that the automatic sample injector is configured, and the test tube rack placed in the sample feeding area of the test tube rack is transported to the sample feeding area of the test tube rack by the automatic sample injector, so that the specific protein analyzer capable of automatically injecting samples is realized.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; within the idea of the invention, also technical features in the above embodiments or in different embodiments may be combined, steps may be implemented in any order, and there are many other variations of the different aspects of the invention as above, which are not provided in detail for the sake of brevity; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (11)
1. A specific protein analyzer, comprising:
the host comprises a shell, and a reaction module, a sampling assembly, a liquid adding assembly, a liquid path system and a control board card which are arranged in the shell, wherein the control board card is used for controlling the reaction module, the sampling assembly, the liquid adding assembly and the liquid path system, the reaction module is used for providing a reaction site of an antigen antibody, the liquid adding assembly is used for adding a reagent into the reaction module, the sampling assembly is used for sucking and spitting a sample and/or an antibody, the liquid path system is connected with the liquid adding assembly and the sampling assembly, and the liquid path system is used for transporting the reagent, the sample and/or the antibody; and
the autosampler, the autosampler includes mixing subassembly, autosampler subassembly and refrigeration storehouse subassembly, the autosampler subassembly includes test-tube rack sample introduction district, test-tube rack feeding district and test-tube rack uninstallation district, the autosampler subassembly be used for with place in the test-tube rack in test-tube rack sample introduction district transports in proper order extremely the test-tube rack feeding district reaches the test-tube rack uninstallation district, the test-tube rack is including the test tube that carries the sample that awaits measuring, the mixing subassembly is used for the mixing the test tube, refrigeration storehouse subassembly is provided with two antibody bottle and places the position.
2. The specific protein analyzer of claim 1, wherein the reaction module comprises a module base, a laser, a diaphragm, a photoelectric conversion plate, a shielding case and a reaction cup set;
the laser, the diaphragm, the reaction cup group, the photoelectric conversion plate and the shielding case are all arranged on the module base and are sequentially arranged along the optical axis of the laser;
the laser is used for emitting laser to the reaction cup group through the diaphragm;
the diaphragm is used for filtering laser emitted by the laser;
the reaction cup group is used for providing a reaction site of antigen and antibody;
the photoelectric conversion plate is used for receiving scattered light of the reaction cup group and converting the scattered light into an electric signal;
the liquid adding assembly is used for adding reagents into the reaction cup group.
3. The specific protein analyzer of claim 2, wherein the reaction module further comprises a stirring motor and a magnet, wherein the magnet is fixedly mounted on a rotating shaft of the stirring motor;
the reaction cup group comprises reaction cups and heating blocks;
the reaction cup is used for providing a reaction site, a magnetic bar stirrer is arranged in the reaction cup, and when the stirring motor drives the magnet to rotate, the magnetic bar stirrer rotates together;
the outer surface of the heating block is covered with a heating film for providing a temperature required for the reaction of the reaction site.
4. The specific protein analyzer of claim 1, wherein the sampling assembly comprises a sampling holder, a piercing carriage, a feed drive, a piercing drive, a sampling needle, and a swab;
the puncture bracket is movably arranged on the sampling fixing seat, the sampling needle is movably arranged on the puncture bracket, and the swab is fixedly arranged on the puncture bracket;
the feeding driving device is used for driving the puncture bracket to move in the horizontal direction;
the puncture driving device is used for driving the sampling needle to move along the vertical direction;
the swab is used to clean the sampling needle as it moves.
5. The specific protein analyzer of claim 1, wherein said liquid feeding assembly comprises a liquid feeding base plate, a liquid feeding tube seat, a liquid feeding tube and a liquid feeding driving device;
the liquid adding pipe seat is movably arranged on the liquid adding bottom plate, and the liquid adding pipe is fixedly arranged on the liquid adding pipe seat;
the liquid feeding driving device is used for driving the liquid feeding pipe seat to move along the feeding direction, so that the liquid feeding pipe is used for adding reagents to the reaction module.
6. The specific protein analyzer of claim 1, wherein the mixing assembly comprises a first linear actuator, a second linear actuator, a rotary actuator, and a clamping jaw;
the first linear driving device is used for driving the clamping jaw to move along the horizontal direction;
the second linear driving device is used for driving the clamping jaw to move along the vertical direction;
the rotary driving device is used for driving the clamping jaws to rotate;
the clamping jaw is used for clamping the test tube.
7. The specific protein analyzer according to claim 6, wherein the first linear driving means comprises a first screw mechanism and a first screw driving motor for driving the second linear driving means to move in the horizontal direction by the first screw mechanism;
the second linear driving device comprises a second screw rod mechanism and a second screw rod driving motor, and the second screw rod driving motor is used for driving the rotary driving device to move along the vertical direction through the second screw rod mechanism;
the rotary driving device comprises a belt wheel mechanism and a belt wheel driving motor, and the belt wheel driving motor is used for driving the clamping jaw to rotate through the belt wheel mechanism.
8. The specific protein analyzer of claim 1, wherein the automated sample assembly further comprises a base plate, a sample assembly, a back plate assembly, a lateral sample assembly, an unload assembly, and a scanner assembly;
the sample feeding assembly comprises a first linear slide rail, a sample feeding pusher dog, a first motor and a first belt pulley assembly, the first linear slide rail is used for guiding the test tube rack to be transported from the sample feeding area of the test tube rack to the feeding area of the test tube rack, the sample feeding pusher dog is used for driving the test tube rack to be transported from the sample feeding area of the test tube rack to the feeding area of the test tube rack, and the first motor is used for driving the sample feeding pusher dog through the first belt pulley assembly;
the rear back plate assembly comprises a rear back plate and a counter arranged on the rear back plate, and the counter is used for counting the test tube positions of the test tube rack;
the transverse sampling assembly comprises a transverse sampling bottom plate, and a second motor, a second linear guide rail, a second belt wheel assembly and a transverse shifting claw assembly which are arranged on the transverse sampling bottom plate, wherein the second linear guide rail is used for guiding the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, the transverse sampling shifting claw is used for driving the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, and the second motor is used for driving the transverse sampling shifting claw through the second belt wheel assembly;
the unloading assembly comprises an unloading support, and a third motor, a third belt wheel assembly, a third linear guide rail and an unloading push plate which are arranged on the unloading support, wherein the third linear guide rail is used for guiding the test tube rack to be unloaded from the test tube unloading area, the unloading push plate is used for driving the test tube rack to be unloaded from the test tube unloading area, and the third motor is used for driving the unloading push plate through the third belt wheel assembly;
the scanner component is used for scanning a bar code attached to the test tube;
the refrigerating bin body is used for refrigerating through the cold end of the Peltier semiconductor, the hot end of the Peltier semiconductor is installed on the radiator, and the radiator radiates heat through the fan.
9. The specific protein analyzer according to any one of claims 1 to 8, wherein the autosampler is provided with a push switch for controlling the sampling assembly to aspirate the sample and/or the antibody.
10. A test method based on the specific protein analyzer as set forth in any one of claims 1 to 9, wherein the test method comprises:
the sampling assembly spits the diluted sample and the antibody to the reaction cup group;
and after the preset time, the reaction module acquires data.
11. The testing method of claim 10, wherein the sampling assembly spits the diluted sample and the antibody to the reaction cup set, specifically comprising:
the sampling component sucks a sample in the test tube;
the sampling assembly moves to a pre-dilution position and spits a sample;
the sampling component moves to the antibody position to absorb the antibody;
the sampling assembly returns to a pre-dilution position to absorb the diluted sample;
the sampling assembly moves to the reaction cup group to spit out the sample and the antibody;
stirring the antigen and antibody in the reaction cup group.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910807096.5ACN110632288A (en) | 2019-08-29 | 2019-08-29 | Specific protein analyzer and testing method based on same |
| PCT/CN2020/091079WO2021036360A1 (en) | 2019-08-29 | 2020-05-19 | Specific protein analyzer and test method based on same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910807096.5ACN110632288A (en) | 2019-08-29 | 2019-08-29 | Specific protein analyzer and testing method based on same |
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| CN110632288Atrue CN110632288A (en) | 2019-12-31 |
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| CN201910807096.5APendingCN110632288A (en) | 2019-08-29 | 2019-08-29 | Specific protein analyzer and testing method based on same |
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| WO (1) | WO2021036360A1 (en) |
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