microorganism inclined plane inoculation deviceTechnical Field
The invention relates to the technical field of microbial inoculation, in particular to a microbial slant inoculation device.
Background
slant inoculation is to inoculate microorganisms from one slant culture medium to another slant culture medium by the following steps: first, the alcohol lamp is ignited. Two test tubes of the strain and the slant medium were placed in parallel on the left hand, and clamped with the thumb and the other four fingers, with the middle finger between the two test tubes, and the orifices of the two test tubes were flush. Second, the tampon was first twisted loose with the right hand and pulled slightly to facilitate withdrawal during inoculation. Thirdly, the inoculating needle is held by the right hand and is heated and sterilized by an alcohol lamp. Fourthly, the tampon on the two test tubes on the left hand is pulled out between the little finger and the palm of the right hand, and the mouth of the test tube is quickly burnt on the alcohol lamp for about 3 seconds, so that a small amount of mixed bacteria possibly stained on the mouth of the test tube can be burnt. Fifthly, the burnt inoculating needle is stretched into the strain tube, lightly contacts with the thalli, a little is taken out, the inoculating needle is slowly pulled out of the test tube, and the needle head part does not touch the tube wall and the tube opening when the inoculating needle is pulled out. Sixth, the inoculating needle is quickly inserted into another tube, and the culture medium is streaked gently to inoculate the bacteria thereon. When in scribing, dense parallel lines are scribed from the bottom to the top so as to fully utilize the area of the inclined plane without scratching the culture medium. Seventh, the test tube orifice is cauterized and the tampon is stoppered. When plugging the tampon, the test tube is not moved to meet the tampon, so that the test tube is prevented from containing air which may contain bacteria during movement. Eighth, the needle is sterilized by burning on a flame. After replacement, the tampon was closed by releasing the hand.
However, the above-mentioned method using artificial slant inoculation has the following problems: 1. higher operation precision requirement is needed in the inoculation process, the inoculation is easy to fail, more hands participate in the process, the bacterial colony is easy to pollute, and 2, the manual inoculation mode wastes time and labor.
disclosure of Invention
The invention aims to solve the problems and provides a microorganism slant inoculation device which increases the automation degree of slant inoculation, has high operation precision and improves the inoculation success rate.
the technical scheme adopted by the invention for solving the technical problems is as follows:
a microorganism slant inoculation device comprises an inoculation platform, two shells, two movable frames, a test tube clamping mechanism and an inoculation needle clamping mechanism, wherein the shells are arranged on the inoculation platform;
The test tube clamping mechanism comprises a first sliding plate, a first connecting plate and a first sleeve, the first sliding plate is connected with the left end movable frame in a sliding mode through a guide rail slider pair, the first sliding plate and the left end movable frame are driven through a lead screw nut, the upper end of the first connecting plate is connected with the first sliding plate, the lower end of the first connecting plate is connected with the first sleeve, a test tube is installed in the first sleeve, and a rotating mechanism is arranged between the test tube and the first sleeve;
The inoculating needle clamping mechanism comprises a second sliding plate, a second connecting plate and a second sleeve, the second sliding plate is connected with the right end movable frame in a sliding mode through a guide rail and slider pair, the second sliding plate and the right end movable frame are driven through a screw nut, the upper end of the second connecting plate is connected with the second sliding plate, the lower end of the second connecting plate is connected with the second sleeve, the inoculating needle is installed in the second sleeve, the inoculating needle is connected with the second sleeve in a sliding mode, an air cylinder is arranged at the upper end of the second sleeve, the end of the air cylinder body is connected with the upper end of the second sleeve, and the end of a piston rod of the air cylinder is connected with the inoculating.
Further, a nut is arranged at the upper end of the movable frame, a long hole is formed in the upper end of the shell, the nut penetrates through the long hole, a lead screw is arranged at the upper end of the shell and is rotatably connected with the shell, a motor is arranged on the side face of the shell, and the output end of the motor is connected with the lead screw.
Further, slewing mechanism includes fixed slot and activity groove, and fixed slot one end is rotated with the activity groove and is connected, and the fixed slot other end can be dismantled with the activity groove and be connected, be equipped with the handle on the fixed slot, rotate between fixed slot and the first sleeve and be connected.
Further, the movable groove end portion is provided with a cavity, a movable rod is arranged in the cavity, a compression spring is arranged on the movable rod, the compression spring is sleeved on the movable rod and provided with a baffle, one end of the compression spring is in contact with the inner wall of the cavity, the other end of the compression spring is in contact with the baffle, a positioning plate is arranged on the handle, a positioning groove is arranged on the positioning plate, and the end portion of the movable rod is matched with the positioning groove.
Furthermore, a clamping groove is formed in the fixing groove, a buckle is arranged at the lower end of the first sleeve, and the clamping groove is matched with the buckle.
Further, rubber layers are arranged in the fixed groove and the movable groove.
Further, be equipped with the holding ring on the test tube, the holding ring cooperates with first sleeve upper end.
furthermore, the upper end of the second sleeve is provided with a mounting plate, the mounting plate is cylindrical, an opening is formed in the mounting plate, and the upper end of the mounting plate is connected with the cylinder body end of the cylinder through a screw.
Furthermore, a stud is arranged at the upper end of the inoculating needle, a threaded hole is formed in the end of the piston rod of the air cylinder, and the stud is matched with the threaded hole.
The invention has the beneficial effects that:
1. The test tube inoculating device comprises an inoculating platform, two shells, two movable frames, a test tube clamping mechanism and an inoculating needle clamping mechanism, wherein the shells are arranged on the inoculating platform; the movable frame is moved about convenient and is disinfected test tube and inoculating needle, prevents bacterial contamination, and good reliability, test tube fixture and inoculating needle fixture can guarantee that test tube and inoculating needle are more stable at the inoculation in-process, prevent to inoculate the in-process and produce the pollution, and good reliability, the operating accuracy is high, has increased the inoculation success rate, has reduced artifical intervention simultaneously, reduces artifically.
2. The inoculating needle clamping mechanism comprises a second sliding plate, a second connecting plate and a second sleeve, wherein the second sliding plate is connected with a right-end movable frame in a sliding mode through a guide rail sliding block pair, the second sliding plate and the right-end movable frame are driven through a screw rod nut, an inoculating needle is installed in the second sleeve, the inoculating needle is connected with the second sleeve in a sliding mode, an air cylinder is arranged at the upper end of the second sleeve, the end of the air cylinder body is connected with the upper end of the second sleeve, the piston rod end of the air cylinder is connected with the inoculating needle, when the inoculating needle clamping mechanism is used, a test tube and the inoculating needle are disinfected through moving the test tube and the inoculating needle, the test tube and the inoculating needle are moved back and forth, when the inoculating needle is overlapped with the axis of the test tube at the rear end, the inoculating needle is driven to move up and down through the expansion of the piston rod of the air, the automation degree is high, the operation precision is high, and the practicality is good.
3. According to the invention, the fixed groove is provided with the clamping groove, the lower end of the first sleeve is provided with the buckle, the clamping groove is matched with the buckle, and after the test tube is tightly held by the movable groove and the fixed groove, the handle is rotated, so that the test tube is rotated, and inoculation is convenient.
4. According to the test tube fixing device, the rubber layers are arranged in the fixed groove and the movable groove, and the rubber layers can better protect the test tube and prevent the test tube from being crushed.
5. According to the invention, the positioning ring is arranged on the test tube, is matched with the upper end of the first sleeve, is made of rubber material and is fixed on the test tube through self friction force, and can prevent the test tube from sliding down from the sleeve.
Drawings
In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a first schematic structural diagram of the present invention;
FIG. 2 is a second schematic structural view of the present invention;
FIG. 3 is a third schematic structural view of the present invention;
FIG. 4 is a cross-sectional view of the test tube holding mechanism of the present invention;
FIG. 5 is a cross-sectional view of the stationary and movable slots of the present invention;
FIG. 6 is a cross-sectional view of an inoculating needle holding mechanism of the present invention.
In the figure: the inoculation platform comprises an inoculation platform 1, a shell 2, a movable frame 3, a first sliding plate 4, a first connecting plate 5, a first sleeve 6, a test tube 7, a nut 8, a long hole 9, a lead screw 10, a motor 11, a fixed groove 12, a movable groove 13, a handle 14, a cavity 15, a movable rod 16, a compression spring 17, a baffle plate 18, a positioning plate 19, a positioning groove 20, a clamping groove 21, a buckle 22, a rubber layer 23, a positioning ring 24, a second sliding plate 25, a second connecting plate 26, a second sleeve 27, an inoculation needle 28, an air cylinder 29, a mounting plate 30, an opening 31 and a stud 32.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiment of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
for convenience of description, a coordinate system is now defined as shown in fig. 1.
As shown in fig. 1 to 3, a microorganism slant inoculation device comprises an inoculation platform 1, a shell 2, two movable frames 3, a test tube clamping mechanism and an inoculation needle clamping mechanism, wherein the shell 2 is mounted on the inoculation platform 1, the two movable frames 3 are arranged, the two movable frames 3 are distributed at the upper end of the shell 2 in a bilateral symmetry manner, the movable frames 3 are connected with the upper end of the shell 2 in a sliding manner, and the movable frames 3 can slide left and right; test tube fixture and inoculation needle fixture can guarantee that test tube and inoculation needle are more stable at the inoculation in-process, prevent to inoculate the in-process and produce the pollution, and the good reliability, the operating accuracy is high, has increased the inoculation success rate, has reduced artifical the intervention simultaneously, reduces artifically.
As shown in fig. 2, 3 and 4, the test tube clamping mechanism includes a first sliding plate 4, a first connecting plate 5 and a first sleeve 6, the first sliding plate 4 is slidably connected with the left end movable frame 3 through a guide rail and slider pair, the first sliding plate 4 and the left end movable frame 3 are driven by a lead screw nut, the sliding direction of the first sliding plate 4 is the front-back direction, the upper end of the first connecting plate 5 is connected with the first sliding plate 4, the lower end of the first connecting plate 5 is connected with the first sleeve 6, a test tube 7 is installed in the first sleeve 6, and a rotating mechanism is arranged between the test tube 7 and the first sleeve 6;
As shown in fig. 2, 3 and 6, the said inoculating needle holding mechanism includes a second sliding plate 25, a second connecting plate 26 and a second sleeve 27, the said second sliding plate 25 is connected with the right end movable frame 3 through a pair of guide rail and slide block, the second sliding plate 25 is driven by a lead screw nut with the right end movable frame 3, the sliding direction of the second sliding plate 25 is front and back, the upper end of the said second connecting plate 26 is connected with the second sliding plate 25, the lower end of the second connecting plate 26 is connected with the second sleeve 27, the inoculating needle 28 is installed in the second sleeve 27, the axis of the test tube 7 is overlapped with the axis of the inoculating needle 28 to ensure that the inoculating needle can be inserted into the test tube, the inoculating needle 28 is connected with the second sleeve 27 through sliding, the upper end of the second sleeve 27 is provided with a cylinder 29, the cylinder 29 end is connected with the upper end of the second sleeve 27, the piston of the cylinder 29 is connected with the inoculating needle 28, when in use, the test tube and the inoculating needle 28 are disinfected by, then the test tube and the inoculating needle 28 are moved back and forth, when the axis of the inoculating needle 28 is overlapped with the axis of the test tube at the rear end, the inoculating needle 28 is driven to move up and down through the stretching of the cylinder piston rod to carry out sampling operation, when the axis of the inoculating needle 28 is overlapped with the axis of the test tube at the front end, the inoculating needle 28 is driven to move up and down through the stretching of the cylinder piston rod to be matched with the rotation of the test tube to carry out inoculating operation, the automation degree is high, the operation precision.
As shown in fig. 1, 3 upper ends of adjustable shelf are equipped with nut 8, 2 upper ends of casing are equipped with rectangular hole 9, nut 8 passes rectangular hole 9, 2 upper ends of casing are equipped with lead screw 10, rotate between lead screw 10 and the casing 2 and be connected, 2 sides of casing are equipped with motor 11, motor 11 output is connected with lead screw 10, lead screw 10 and motor one-to-one, the distribution of all symmetries is at 2 both ends of casing, every adjustable shelf corresponds a lead screw, rotate and then control about the drive adjustable shelf through the motor drive lead screw, control about test tube and inoculation needle, conveniently disinfect test tube and inoculation needle, degree of automation is high.
As shown in fig. 3 and 5, the rotating mechanism includes a fixed groove 12 and a movable groove 13, one end of the fixed groove 12 is rotatably connected with the movable groove 13, the other end of the fixed groove 12 is detachably connected with the movable groove 13, a handle 14 is arranged on the fixed groove 12, and the fixed groove 12 is rotatably connected with the first sleeve 6.
As shown in fig. 5, the tip of the movable groove 13 is provided with a cavity 15, a movable rod 16 is arranged in the cavity 15, a compression spring 17 is arranged on the movable rod 16, the compression spring 17 is sleeved on the movable rod 16, a baffle 18 is arranged on the movable rod 16, one end of the compression spring 17 is in contact with the inner wall of the cavity 15, the other end of the compression spring 17 is in contact with the baffle 18, a positioning plate 19 is arranged on the handle 14, a positioning groove 20 is arranged on the positioning plate 19, the tip of the movable rod 16 is matched with the positioning groove 20, the tip of the movable rod 16 is provided with an inclined surface, when the test tube is used, the movable groove 13 rotates the movable groove 13, the tip.
As shown in fig. 4, be equipped with draw-in groove 21 on the fixed slot 12, first sleeve 6 lower extreme is equipped with buckle 22, draw-in groove 21 and buckle 22 cooperation, and movable groove 13 holds the test tube with fixed slot 12 after, thereby rotates handle 14 and makes the test tube rotate, convenient inoculation.
As shown in fig. 4 and 5, the rubber layer 23 is disposed in both the fixed groove 12 and the movable groove 13, and the rubber layer 23 can better protect the test tube and prevent the test tube from being crushed.
As shown in fig. 2 and 4, the test tube 7 is provided with a positioning ring 24, the positioning ring 24 is matched with the upper end of the first sleeve 6, the positioning ring 24 is made of rubber material and fixed on the test tube by self friction, and the positioning ring 24 can prevent the test tube from sliding down from the sleeve 6.
As shown in figures 2 and 6, the upper end of the second sleeve 27 is provided with a mounting plate 30, the mounting plate 30 is cylindrical, the mounting plate 30 is provided with an opening 31, the upper end of the mounting plate 30 is connected with the cylinder body end of the cylinder 29 through a screw, and the opening 31 is convenient for observing the connection condition of the inoculating needle.
As shown in fig. 6, the upper end of the inoculating needle 28 is provided with a stud 32, the piston rod end of the air cylinder 29 is provided with a threaded hole, and the stud 32 is matched with the threaded hole.
In the description of the present invention, it should be noted that the terms "left", "right", "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; they may be mechanically or electrically connected, directly or indirectly through intervening media, or may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.