技术领域technical field
本发明涉及医药仪器技术领域，具体为一种薄层色谱分析仪。The invention relates to the technical field of medical instruments, in particular to a thin-layer chromatography analyzer.
背景技术Background technique
薄层色谱法是一种常用的微量而快速的色谱法，它具有耗时少、灵敏度高、操作方便等优点，因而在化工，医药等领域得到广泛应用。其主要工作原理是在载板上(玻璃板、塑料板等有一定强度及化学惰性的平面板)将吸附剂均匀涂成一薄层，再通过毛细管吸取试样溶液并将其精准地点在距离载板一端1-2厘米的直线上。待溶剂挥发后将载板放入已经预饱和的展开缸中，由于毛细管作用，试样溶液在薄层板上缓慢前进，前进至一定距离后，取出载板，样品组分因移动速度不同而彼此分离形成不同距离的斑点。最后使用紫外光灯照射或显色剂检出斑点，根据比移值(Retardation factor，Rf)对试样溶液的成分进行定量分析。Thin-layer chromatography is a commonly used trace and rapid chromatography. It has the advantages of less time-consuming, high sensitivity, and convenient operation, so it is widely used in chemical, pharmaceutical and other fields. Its main working principle is to evenly coat the adsorbent into a thin layer on the carrier plate (glass plate, plastic plate, etc., which has a certain strength and chemical inertness), and then absorb the sample solution through the capillary and accurately place it on the load at a distance. On a straight line 1-2 cm from one end of the board. After the solvent evaporates, put the carrier plate into the pre-saturated developing cylinder. Due to capillary action, the sample solution slowly advances on the thin-layer plate. After advancing to a certain distance, take out the carrier plate. The spots are separated from each other to form different distances. Finally, the spot is detected by ultraviolet light irradiation or color developer, and the composition of the sample solution is quantitatively analyzed according to the ratio shift value (Retardation factor, Rf).
薄层色谱分析的结果主要受温度、湿度、点样的准确性等因素的影响。温度影响各有机溶剂的蒸发程度和展开缸中蒸汽比例，进而影响含水展开剂两相的溶解度，改变溶剂的比例；湿度主要影响薄层板的吸附能力，在不同的相对湿度下可以达到不同的吸附平衡；点样量过大将降低分离效率，并且错误的点样操作会对薄层表面造成损伤，极大地影响色谱的质量。The results of TLC analysis are mainly affected by factors such as temperature, humidity, and the accuracy of sample placement. The temperature affects the evaporation degree of each organic solvent and the steam ratio in the developing cylinder, which in turn affects the solubility of the two phases of the aqueous developing agent and changes the ratio of the solvent; the humidity mainly affects the adsorption capacity of the thin-layer plate, and different relative humidity can be achieved. Adsorption balance; if the sample volume is too large, the separation efficiency will be reduced, and the wrong sample application operation will cause damage to the surface of the thin layer, which will greatly affect the quality of the chromatogram.
现有薄层色谱分析操作方法中存在以下问题：The following problems exist in the existing thin-layer chromatographic analysis operation method:
1.人工操作受限于操作者的经验及精准度，将不可避免地造成误差，并且整个操作过程处于开放空间中，环境的湿度和温度都将影响薄层色谱分析结果。1. Manual operation is limited by the experience and accuracy of the operator, which will inevitably cause errors, and the entire operation process is in an open space, and the humidity and temperature of the environment will affect the results of TLC analysis.
2.人工操作时需与溶剂近距离接触，部分有毒有害溶剂的挥发会对操作者的健康造成危害。2. Manual operation needs to be in close contact with solvents, and the volatilization of some toxic and harmful solvents will cause harm to the health of the operator.
3.现有薄层色谱分析仪器(如：自动点样仪、自动展开仪、喷雾系统等)大多是将薄层色谱分析过程的某一步骤实现自动化，仍旧需要操作者将载板从仪器中取出再放置于另一仪器中，此过程易于受到环境温度与湿度的影响导致得出的色谱质量较差，并且需要操作者全程陪同，操作繁琐，效率低。3. Most of the existing thin-layer chromatography analysis instruments (such as: automatic sample spotting instrument, automatic unfolding instrument, spray system, etc.) automate a certain step of the thin-layer chromatography analysis process, and the operator still needs to remove the carrier plate from the instrument. Take it out and place it in another instrument. This process is easily affected by the ambient temperature and humidity, resulting in poor chromatographic quality, and requires the operator to accompany the whole process, which is cumbersome and inefficient.
为解决上述问题，设计了一种薄层色谱分析仪，高度集成点样、干燥、展开、显色等功能于一体，实现薄层色谱分析操作全自动化，具有体积小，操作简便、效率高、安全性强等优点。In order to solve the above problems, a TLC analyzer is designed, which is highly integrated with the functions of sample pointing, drying, development, and color development, and realizes full automation of TLC analysis operations. It has the advantages of small size, easy operation, high efficiency, Strong security and other advantages.
发明内容Contents of the invention
本发明的目的是提供一种薄层色谱分析仪，以解决上述背景中提出的问题。The object of the present invention is to provide a thin-layer chromatographic analyzer to solve the problems raised in the above-mentioned background.
为实现上述目的，本发明提供如下技术方案：一种薄层色谱分析仪，包括恒温箱、固定架、支柱和隔膜泵，其特征在于：所述恒温箱右侧表面固定有固定支座，所述固定支座与右侧门铰接，所述恒温箱左侧铰接有密封门，所述恒温箱顶部固定安装有紫外灯和白炽灯，所述恒温箱底部固定连接有固定架，所述固定架底部通过风机支座将热风机固定，所述固定架上部固定连接滑轨4，所述滑轨4右侧安装有电机5，所述电机5输出轴通过联轴器4与丝杆4连接，所述丝杆4安装有滑台4，所述滑台4与底板固定连接，所述底板上固定安装有齿轮换向箱和电机4，所述底板和旋转板转动连接，所述旋转板上固定连接电动液压缸的一端，所述电动液压缸另一端与载板夹固定连接，所述支柱固定在恒温箱底部，所述支柱顶部固定安装滑轨2，所述滑轨2左侧安装有电机2，所述电机2输出轴通过联轴器2与丝杆2连接，所述丝杆2安装有滑台2，所述滑台2固定连接滑轨3，所述滑轨3顶部安装有电机3，所述电机3输出轴通过联轴器3与丝杆2连接，所述丝杆3安装有滑台3，所述滑台3固定连接有电动夹爪，所述固定架旁有滑轨1固定于恒温箱底部，所述滑轨1一端安装有电机1，所述电机1输出轴通过联轴器1与丝杆1连接，所述丝杆1安装有滑台1，所述滑台1固定连接有试管架，所述隔膜泵固定安装在恒温箱内壁。In order to achieve the above object, the present invention provides the following technical solutions: a thin-layer chromatographic analyzer, comprising a constant temperature box, a fixed frame, a pillar and a diaphragm pump, characterized in that: a fixed support is fixed on the right side surface of the constant temperature box, and the The fixed support is hinged with the right door, the left side of the incubator is hinged with a sealed door, the top of the incubator is fixedly equipped with an ultraviolet lamp and an incandescent lamp, and the bottom of the incubator is fixedly connected with a fixing frame. The hot air blower is fixed at the bottom through the fan support, and the upper part of the fixed frame is fixedly connected with the slide rail 4, and the motor 5 is installed on the right side of the slide rail 4, and the output shaft of the motor 5 is connected with the screw rod 4 through the coupling 4, The screw mandrel 4 is equipped with a slide table 4, the slide table 4 is fixedly connected with the base plate, the gear reversing box and the motor 4 are fixedly installed on the base plate, the base plate and the rotating plate are rotatably connected, and the rotating plate One end of the electro-hydraulic cylinder is fixedly connected, the other end of the electro-hydraulic cylinder is fixedly connected with the carrier plate clamp, the pillar is fixed at the bottom of the incubator, the top of the pillar is fixedly installed with a slide rail 2, and the left side of the slide rail 2 is installed with Motor 2, the output shaft of the motor 2 is connected to the screw rod 2 through the coupling 2, the screw rod 2 is equipped with a slide table 2, the slide table 2 is fixedly connected to the slide rail 3, and the top of the slide rail 3 is installed with Motor 3, the output shaft of the motor 3 is connected to the screw rod 2 through a coupling 3, the screw rod 3 is equipped with a slide table 3, and the slide table 3 is fixedly connected with an electric gripper, and there is a sliding table next to the fixed frame. The rail 1 is fixed on the bottom of the incubator, and one end of the slide rail 1 is equipped with a motor 1, and the output shaft of the motor 1 is connected with the screw rod 1 through a coupling 1, and the screw rod 1 is equipped with a slide table 1, and the slide Table 1 is fixedly connected with a test tube rack, and the diaphragm pump is fixedly installed on the inner wall of the incubator.
优选的，所述恒温箱内设有隔板，所述恒温箱内部固定有加湿器，所述恒温箱顶部固定有观测镜，所述恒温箱表面固定有温湿度显示器，所属恒温箱一角有一矩形槽，槽内放置有显色剂杯。Preferably, a clapboard is provided inside the thermostat, a humidifier is fixed inside the thermostat, an observation mirror is fixed on the top of the thermostat, a temperature and humidity display is fixed on the surface of the thermostat, and there is a rectangle at one corner of the thermostat Groove, the developer cup is placed in the groove.
优选的，所述固定架顶层中间设有滑槽，所述固定架左端留有空位放置有展开缸，所述固定架左端安装有弹簧，所述展开缸盖后端设有两孔，前端有与载板夹相同宽度的槽，所述展开缸盖通过弹簧紧紧压在固定架上，与展开缸形成密闭的空间。Preferably, a chute is provided in the middle of the top layer of the fixed frame, there is a space at the left end of the fixed frame to place an expansion cylinder, a spring is installed at the left end of the fixed frame, two holes are provided at the rear end of the expansion cylinder cover, and there are two holes at the front end. The slot with the same width as the carrier clamp, the expansion cylinder cover is tightly pressed on the fixed frame by a spring to form a closed space with the expansion cylinder.
优选的，所述热风机与风管密封连接，所述风管沿固定架侧边的孔伸出。Preferably, the hot air blower is sealed and connected with an air duct, and the air duct protrudes along the hole on the side of the fixing frame.
优选的，所述载板夹上设有刻度，所述载板夹侧边装有伸缩杆。Preferably, scales are provided on the carrier clamp, and telescopic rods are installed on the sides of the carrier clamp.
优选的，所述隔膜泵左端密封连接有出水管，所述出水管由管道支架固定在隔板上，尾端垂直向下且有规则分布的微孔，所述隔膜泵的右端密封连接有吸水管，所述吸水管从恒温箱的孔穿出，末端伸入显色剂杯内。Preferably, the left end of the diaphragm pump is sealed and connected with an outlet pipe, the outlet pipe is fixed on the separator by a pipe bracket, the tail end is vertically downward and has regularly distributed micropores, and the right end of the diaphragm pump is sealed and connected with a water suction pipe. Tube, the water suction tube passes through the hole of the incubator, and the end extends into the developer cup.
优选的，所述固定支座、支柱、管道支架、弹簧、风机支座各有两个。Preferably, there are two fixed supports, pillars, pipe supports, springs, and fan supports.
与现有技术相比，本发明的有益效果是：Compared with prior art, the beneficial effect of the present invention is:
1、将薄层色谱分析过程中点样、展开、干燥和显色等步骤实现全自动化操作，并高度集成于恒温箱，有效降低人工操作或单一功能仪器逐步操作的繁琐性，具有体积小，操作简便的优点。1. The steps of sampling, development, drying and color development in the thin-layer chromatography analysis process are fully automated, and are highly integrated in the incubator, which effectively reduces the cumbersomeness of manual operation or step-by-step operation of a single-function instrument. It has a small size, The advantages of easy operation.
2、整个操作过程都在密闭的恒温箱中，有效的避免不同环境下温度和湿度对分析的影响。装置采用高精度滑台与电动夹爪配合，使得点样间距精准，面积恒定、点样量准确，并且减少对载板造成的物理损伤，进一步提高了薄层色谱分析的精准性，操作得出的色谱质量高。2. The whole operation process is in a closed incubator, effectively avoiding the influence of temperature and humidity in different environments on the analysis. The device uses a high-precision slide table and electric grippers to make the spotting distance accurate, the area constant, the spotting volume accurate, and reduce the physical damage to the carrier plate, which further improves the accuracy of thin-layer chromatography analysis. The chromatographic quality is high.
3、装置采用伸缩式载板夹，可适应多种规格载板。采用电动夹爪夹取毛细管点样方式，可对多种溶剂同时进行分离，并且做到一根毛细管吸取一种溶剂，避免溶剂混合而对分析结果产生影响。内部热风机能够加快风干的同时避免了有毒有害溶剂挥发对人体造成伤害，具有效率高，安全性强的优点。3. The device adopts a telescopic carrier plate clamp, which can adapt to various specifications of carrier plates. The electric gripper is used to pick up the capillary tube sampling method, which can separate multiple solvents at the same time, and one capillary tube can absorb one solvent, so as to avoid the influence of solvent mixing on the analysis results. The internal hot air blower can speed up the air drying while avoiding the harm to the human body caused by the volatilization of toxic and harmful solvents. It has the advantages of high efficiency and strong safety.
附图说明Description of drawings
图1为本发明中恒温箱示意图；Fig. 1 is a schematic diagram of an incubator in the present invention;
图2为本发明中内部结构左侧示意图；Fig. 2 is the left schematic diagram of internal structure among the present invention;
图3为本发明中滑台组示意图；Fig. 3 is a schematic diagram of the sliding platform group in the present invention;
图4为本发明中内部结构右侧示意图；Fig. 4 is a schematic view on the right side of the internal structure in the present invention;
图5为本发明中固定架示意图；Fig. 5 is a schematic diagram of a fixed mount in the present invention;
图6为本发明中固定架剖视示意图；Fig. 6 is a schematic cross-sectional view of a fixing frame in the present invention;
图7为本发明中载板夹示意图；Fig. 7 is a schematic diagram of the carrier clip in the present invention;
图8为本发明中展开缸盖示意图；Fig. 8 is a schematic diagram of the expanded cylinder head in the present invention;
图9为本发明中旋转板示意图；Fig. 9 is a schematic diagram of a rotating plate in the present invention;
图10为本发明中展开缸示意图；Figure 10 is a schematic diagram of the deployment cylinder in the present invention;
图中：7—伸缩杆 10—恒温箱 11—观测镜 12—右侧门 13—固定支座 14—显色剂杯 15—温湿度显示器 21—密封门 22—隔板 23—紫外灯 24—加湿器 25—白炽灯30—电机1 31—丝杆1 32—轴承座1 33—滑台1 34—滑轨1 35—毛细管 36—试管架37—试管 38—电机2 39—联轴器2 310—丝杆2 311—轴承座2 312—滑轨2 313—滑台2314—电机3 315—联轴器3 316—丝杆3 317—滑轨3 318—滑台3 319—电动夹爪 320—支柱 321—联轴器1 40—管道支架 41—吸水管 42—隔膜泵 43—出水管 50—弹簧 51—展开缸盖 52—齿轮换向箱 53—电机4 54—电动液压缸 55—载板 56—载板夹 57—旋转板 58—底板 59—固定架 60—展开缸 61—丝杆4 62—滑轨4 63—风管 64—风机支座65—滑台466—热风机 67—联轴器4 68—电机5。Among the figure: 7—telescopic rod 10—constant temperature box 11—observation mirror 12—right side door 13—fixed support 14—developer cup 15—temperature and humidity display 21—sealed door 22—partition 23—ultraviolet lamp 24— Humidifier 25—incandescent lamp 30—motor 1 31—screw rod 1 32—bearing seat 1 33—sliding table 1 34—sliding rail 1 35—capillary 36—test tube rack 37—test tube 38—motor 2 39—coupling 2 310—screw 2 311—bearing housing 2 312—slide rail 2 313—slide table 2314—motor 3 315—coupling 3 316—screw rod 3 317—slide rail 3 318—slide table 3 319—electric gripper 320 —pillar 321—coupling 1 40—pipe support 41—suction pipe 42—diaphragm pump 43—outlet pipe 50—spring 51—expand cylinder head 52—gear reversing box 53—motor 4 54—electric hydraulic cylinder 55—load Plate 56—carrier clamp 57—rotating plate 58—bottom plate 59—fixed frame 60—expansion cylinder 61—screw rod 4 62—slide rail 4 63—air duct 64—fan support 65—sliding table 466—hot air blower 67— Coupling 4 68—motor 5.
具体实施方式Detailed ways
下面将结合本发明实施例中的附图，对本发明中的技术方案进行清楚、完整的描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其实施例，都属于本发明保护的范围。The technical solutions in the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all the embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
请参阅图1至图10，本发明提供的一种技术方案：包括恒温箱、固定架、支柱和隔膜泵，其特征在于：所述恒温箱右侧表面固定有固定支座，所述固定支座与右侧门铰接，所述恒温箱左侧铰接有密封门，所述恒温箱内设有隔板，所述恒温箱内部固定安装有加湿器，所述恒温箱顶部固定有观测镜，所述恒温箱表面固定有温湿度显示器，所属恒温箱一角有一矩形槽，槽内放置有显色剂杯，所述恒温箱顶部固定安装有紫外灯和白炽灯，所述恒温箱底部固定连接有固定架，所述固定架底部通过风机支座将热风机固定，所述热风机与风管密封连接，所述风管沿固定架侧边的孔伸出，所述固定架上部固定连接滑轨4，所述滑轨4右侧安装有电机5，所述电机5输出轴通过联轴器4与丝杆4连接，所述丝杆4安装有滑台4，所述滑台4与底板固定连接，所述底板上安装有齿轮换向箱和电机4，所述底板和旋转板转动连接，所述旋转板上固定连接电动液压缸的一端，所述电动液压缸另一端与载板夹固定连接，所述载板夹，上设有刻度，所述载板夹，侧边装有伸缩杆，所述支柱固定在恒温箱底部，所述支柱顶部固定安装滑轨2，所述滑轨2左侧安装有电机2，所述电机2输出轴通过联轴器2与丝杆2连接，所述丝杆2安装有滑台2，所述滑台2固定连接滑轨3，所述滑轨3顶部安装有电机3，所述电机3输出轴通过联轴器3与丝杆2连接，所述丝杆3安装有滑台3，所述滑台3固定连接有电动夹爪，所述固定架旁有滑轨1固定于恒温箱底部，所述滑轨1一端安装有电机1，所述电机1输出轴通过联轴器1与丝杆1连接，所述丝杆1安装有滑台1，所述滑台1固定连接有试管架，所述隔膜泵固定安装在恒温箱内壁，所述隔膜泵左端密封连接有出水管，所述出水管由管道支架固定在隔板上，尾端垂直向下且有规则分布的微孔，所述隔膜泵的右端密封连接有吸水管，所述吸水管从恒温箱的孔穿出，末端伸入显色剂杯内。Please refer to Fig. 1 to Fig. 10, a technical solution provided by the present invention: comprising a constant temperature box, a fixed frame, a pillar and a diaphragm pump, characterized in that: a fixed support is fixed on the right side surface of the constant temperature box, and the fixed support The seat is hinged with the right door, the left side of the incubator is hinged with a sealed door, the incubator is provided with a partition, the inside of the incubator is fixed with a humidifier, and the top of the incubator is fixed with an observation mirror. A temperature and humidity indicator is fixed on the surface of the incubator, and there is a rectangular groove in one corner of the incubator, and a developer cup is placed in the groove. The top of the incubator is fixed with an ultraviolet lamp and an incandescent lamp, and the bottom of the incubator is fixedly connected with a fixed frame, the bottom of the fixed frame fixes the hot air blower through the fan support, the hot air blower is sealed and connected with the air duct, the air duct protrudes along the hole on the side of the fixed frame, and the upper part of the fixed frame is fixedly connected to the slide rail 4 , the right side of the slide rail 4 is equipped with a motor 5, the output shaft of the motor 5 is connected with the screw rod 4 through the coupling 4, the screw rod 4 is equipped with a slide table 4, and the slide table 4 is fixedly connected with the bottom plate , the gear reversing box and the motor 4 are installed on the base plate, the base plate is connected in rotation with the rotating plate, one end of the electric hydraulic cylinder is fixedly connected to the rotating plate, and the other end of the electric hydraulic cylinder is fixedly connected with the carrier plate clamp , the carrier plate clamp is provided with a scale, the side of the carrier plate clamp is equipped with a telescopic rod, the pillar is fixed at the bottom of the incubator, the top of the pillar is fixedly installed with a slide rail 2, and the left side of the slide rail 2 A motor 2 is installed on the side, and the output shaft of the motor 2 is connected to the screw rod 2 through a coupling 2. The screw rod 2 is equipped with a slide table 2, and the slide table 2 is fixedly connected to a slide rail 3, and the slide rail 3 A motor 3 is installed on the top, and the output shaft of the motor 3 is connected to the screw rod 2 through a coupling 3. The screw rod 3 is equipped with a slide table 3, and the slide table 3 is fixedly connected with an electric gripper. There is a slide rail 1 fixed on the bottom of the incubator, one end of the slide rail 1 is equipped with a motor 1, the output shaft of the motor 1 is connected to the screw rod 1 through a coupling 1, and the screw rod 1 is equipped with a slide table 1, The slide table 1 is fixedly connected with a test tube rack, the diaphragm pump is fixedly installed on the inner wall of the constant temperature box, and the left end of the diaphragm pump is sealed and connected with a water outlet pipe, and the water outlet pipe is fixed on the partition by a pipe bracket, and the tail end is vertical to There are regularly distributed micropores, and the right end of the diaphragm pump is sealed with a water suction pipe. The water suction pipe passes through the hole of the thermostat, and the end of the water suction pipe extends into the developer cup.
工作原理：该薄层色谱分析仪工作时，首先打开密封门(21)将展开缸(60)取出，倒入适当展开剂后沿固定架(59)左侧推到底，关闭密封门(21)。初始状态下，固定架(59)上的展开缸盖(51)在弹簧(50)的作用下与固定架(59)紧紧贴合，并与展开缸(60)形成一个密闭空间，使展开剂在展开缸(60)内充分饱和。打开右侧门(12)，此时旋转板(57)处于水平位置，将载板(55)放至载板夹(56)上，顶部对齐，通过伸缩杆(7)将载板(55)紧紧卡住，关闭右侧门(12)后加湿器(24)启动，当恒温箱(10)内湿度到达指定数值时加湿器(24)关闭。随后电机5(68)启动，通过联轴器4(67)带动丝杆4(61)转动，从而使滑台4(65)沿着滑轨4(62)直线移动。滑台4(65)带着与之固定连接的底板(58)移动，同时底板(58)上的旋转板(57)、载板夹(56)和载板(55)同步移动。移动至指定位置后，电机5(68)关闭，电机1(30)启动，通过联轴器1(321)带动丝杆1(31)转动，使滑台1(33)沿着滑轨1(34)直线移动。滑台1(33)带动与之固定连接的试管架(36)移动至指定位置后电机1(30)关闭。电机2(38)启动，通过联轴器2(39)带动丝杆2(310)转动，使滑台2(313)沿着滑轨2(312)直线移动，当电动夹爪(319)处于试管架(36)上指定位置后电机2(38)关闭。电机3(314)启动，通过联轴器3(315)带动丝杆3(316)转动，使滑台3(318)沿着滑轨3(317)直线移动。滑台3(318)带动电动夹爪(319)下降至指定位置后，夹取毛细管(35)，随后滑台3(318)带动电动夹爪(319)上升一定距离，电机3(314)关闭。接着电机1(30)和电机2(38)分别带着滑台1(33)和滑台2(313)移动，当目标试管(37)正处于电动夹爪(319)正下方时电机1(30)和电机2(38)关闭。电机3(314)启动，滑台3(318)带着电动夹爪(319)往下移，使毛细管(35)伸入目标试管(37)内吸取溶剂，吸取完成后，滑台3(318)带动电动夹爪(319)上升至指定位置后电机3(314)关闭。电机2(38)启动，使滑台2(313)带着电动夹爪(319)水平横移至载板(55)正上方。随后，电机2(38)和电机3(314)相互配合，控制电动夹爪(319)上下左右移动，使毛细管(35)在载板(55)按照需求上进行精准的点样。点样完成后，电机1(30)、电机2(38)和电机3(314)分别移动滑台1(33)、滑台2(313)和滑台3(318)将毛细管(35)放回试管架(36)原位。若要在载板(55)进行多种溶剂点样，多次重复上述操作即可。热风机(66)启动，通过风管(63)将热风吹向载板(55)使溶剂加快水分挥发，随后关闭热风机(66)。电机5(68)启动，通过滑台4(65)带动底板(58)移动至固定架(59)滑槽的最左端，底板(58)向左移动同时会将展开缸盖(51)向左推动，此时展开缸盖(51)在展开缸(60)顶部形成一个矩形凹槽。关闭电机5(68)后启动电机4(53)，通过齿轮换向箱(52)将旋转板(57)旋转90度后，关闭电机4(53)。接着电动液压缸(54)向下推带着载板夹(56)和载板(55)伸入展开缸(60)内，此时载板(55)底部与展开缸(60)内溶剂接触，载板夹(56)上部刚好与矩形槽重合，从而与展开缸(60)形成密闭的空间，进行溶剂的展开。展开完成后，电动液压缸(54)带动载板夹(56)和载板(55)缩回，电机4(53)启动，通过齿轮换向箱(52)将旋转板(57)旋转至水平位置后关闭电机4(53)。电机5(68)启动，通过滑台4(65)带着底板(58)移动，直到载板(55)处于风管(63)出口位置，关闭电机5(68)，此时展开缸盖(51)在弹簧(50)作用下自动回到与固定架(59)贴合位置。热风机(66)启动，通过风管(63)吹出热风对载板(55)进行干燥。干燥完成后关闭热风机(66)，启动电机5(68)，通过滑台4(65)带着底板(58)向右直线移动，若试样溶剂需要显色剂显色，则在载板(55)移动至出水管(43)尾端下方时，隔膜泵(42)通过吸水管(41)从显色剂杯(14)中吸取显色剂，再从出水管(43)尾端以喷雾的形式喷出，滑台4(65)保持匀速直线移动，所以显色剂能均匀的喷在载板(55)上，在载板(55)完全通过出水管(43)下方后，关闭隔膜泵(42)。滑台4(65)移动至固定架(59)滑槽最右端时关闭电机5(68)。开启紫外灯(23)，通过观测镜(11)观察载板(55)上溶剂展开结果。Working principle: when the TLC analyzer is working, first open the airtight door (21) to take out the expansion cylinder (60), pour in a suitable developer and push it to the bottom along the left side of the fixing frame (59), and close the airtight door (21) . In the initial state, the expansion cylinder cover (51) on the fixed frame (59) is closely attached to the fixed frame (59) under the action of the spring (50), and forms a closed space with the expansion cylinder (60), so that the expansion The agent is fully saturated in the deployment cylinder (60). Open the right side door (12), at this time the rotating plate (57) is in the horizontal position, put the carrier plate (55) on the carrier plate clamp (56), align the top, and use the telescopic rod (7) to place the carrier plate (55) Clamp tightly, after closing the right side door (12), the humidifier (24) starts, and when the humidity in the incubator (10) reaches the specified value, the humidifier (24) closes. Motor 5 (68) starts subsequently, drives screw mandrel 4 (61) to rotate by shaft coupling 4 (67), thereby slide table 4 (65) moves linearly along slide rail 4 (62). The slide table 4 (65) moves with the base plate (58) fixedly connected therewith, and the rotating plate (57) on the base plate (58), the carrier plate clamp (56) and the carrier plate (55) move synchronously. After moving to the designated position, the motor 5 (68) is turned off, the motor 1 (30) is started, and the screw rod 1 (31) is driven to rotate through the coupling 1 (321), so that the slide table 1 (33) moves along the slide rail 1 ( 34) Move in a straight line. The slide table 1 (33) drives the test tube rack (36) fixedly connected therewith to move to the designated position and then the motor 1 (30) is closed. The motor 2 (38) is started, and the screw rod 2 (310) is driven to rotate through the shaft coupling 2 (39), so that the slide table 2 (313) moves linearly along the slide rail 2 (312). When the electric gripper (319) is in the Motor 2 (38) is closed after designated position on the test tube rack (36). The motor 3 (314) starts, drives the screw mandrel 3 (316) to rotate through the shaft coupling 3 (315), and makes the slide table 3 (318) move linearly along the slide rail 3 (317). Slider 3 (318) drives the electric gripper (319) down to the specified position, then clamps the capillary (35), and then slide 3 (318) drives the electric gripper (319) up a certain distance, and the motor 3 (314) is turned off . Then motor 1 (30) and motor 2 (38) move with slide table 1 (33) and slide table 2 (313) respectively, when target test tube (37) is just under electric gripper jaw (319) when motor 1 ( 30) and motor 2 (38) off. The motor 3 (314) starts, and the slide table 3 (318) moves down with the electric gripper (319), so that the capillary (35) is stretched into the target test tube (37) to absorb the solvent. After the suction is completed, the slide table 3 (318 ) drives the electric gripper (319) to rise to the designated position and then the motor 3 (314) is closed. The motor 2 (38) is started, so that the slide table 2 (313) horizontally moves to the top of the carrier plate (55) with the electric gripper (319). Subsequently, the motor 2 (38) and the motor 3 (314) cooperate with each other to control the electric gripper (319) to move up, down, left, and right, so that the capillary (35) can accurately spot samples on the carrier plate (55) as required. After sample spotting is completed, motor 1 (30), motor 2 (38) and motor 3 (314) move slide table 1 (33), slide table 2 (313) and slide table 3 (318) respectively to place capillary (35) Return test tube rack (36) original position. To perform spotting of various solvents on the carrier plate (55), the above operation can be repeated several times. The hot air blower (66) starts, and the hot air is blown to the carrier plate (55) by the air duct (63) to make the solvent quicken the volatilization of moisture, and then the hot air blower (66) is closed. The motor 5 (68) is started, and the base plate (58) is driven to move to the leftmost end of the chute of the fixed frame (59) through the slide table 4 (65). Push, and now the cylinder cover (51) is deployed to form a rectangular groove on the top of the cylinder (60). Start motor 4 (53) after closing motor 5 (68), after rotating plate (57) is rotated 90 degrees by gear reversing box (52), close motor 4 (53). Then the electro-hydraulic cylinder (54) pushes down and stretches the carrier plate clamp (56) and the carrier plate (55) into the deployment cylinder (60), at this time the bottom of the carrier plate (55) is in contact with the solvent in the deployment cylinder (60) , the upper part of the carrier clamp (56) just coincides with the rectangular groove, thereby forming a closed space with the expansion cylinder (60) for the expansion of the solvent. After the deployment is completed, the electric hydraulic cylinder (54) drives the carrier plate clamp (56) and the carrier plate (55) to retract, the motor 4 (53) is started, and the rotating plate (57) is rotated to the horizontal position through the gear reversing box (52) Turn off motor 4 (53) after position. The motor 5 (68) starts, moves with the base plate (58) through the slide table 4 (65), until the carrier plate (55) is at the outlet position of the air duct (63), closes the motor 5 (68), and now the cylinder head ( 51) Under the action of the spring (50), it automatically returns to the joint position with the fixing frame (59). The hot air blower (66) is started, and the hot air is blown out by the air duct (63) to dry the carrier plate (55). After the drying is completed, turn off the hot air blower (66), start the motor 5 (68), and move straight to the right with the base plate (58) through the slide table 4 (65). (55) When moving below the tail end of the water outlet pipe (43), the diaphragm pump (42) sucks the color developer from the color developer cup (14) through the water suction pipe (41), and then from the tail end of the water outlet pipe (43) to Spray in the form of a spray, the sliding table 4 (65) keeps moving in a straight line at a constant speed, so the color developer can be evenly sprayed on the carrier plate (55), and after the carrier plate (55) completely passes under the outlet pipe (43), close the Diaphragm pump (42). Slide table 4 (65) closes motor 5 (68) when moving to fixed mount (59) chute rightmost. Turn on the ultraviolet lamp (23), and observe the solvent development result on the support plate (55) through the observation mirror (11).
尽管已经示出和描述了本发明的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变形，本发明的范围由所附权利要求书及其等同物限定。Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910949902.2ACN110531023B (en) | 2019-10-08 | 2019-10-08 | Thin layer chromatography analyzer |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910949902.2ACN110531023B (en) | 2019-10-08 | 2019-10-08 | Thin layer chromatography analyzer |
| Publication Number | Publication Date |
|---|---|
| CN110531023Atrue CN110531023A (en) | 2019-12-03 |
| CN110531023B CN110531023B (en) | 2024-06-14 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910949902.2AActiveCN110531023B (en) | 2019-10-08 | 2019-10-08 | Thin layer chromatography analyzer |
| Country | Link |
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| CN (1) | CN110531023B (en) |
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