A kind of chemical luminescence detector and its application methodTechnical field
The present invention relates to a kind of chemical luminescence detector and its application methods, belong to medical detection technology.
Background technique
Chemical luminescence detector is the immune response that will have highly sensitive chemical luminescent detecting technology and high specificIt combines, the detection and analysis technology for various antigens, haptens, antibody, hormone, enzyme, fatty acid, vitamin and drug etc..Be grow up after radioimmunology analysis, enzyme exempt from analysis, fluoroimmunoassay and time-resolved fluoroimmunoassay one mostNew immunoassay.
Traditional chemical luminescence detector has liquid channel system to need contact liq, high failure rate, needs to use in detection processTo liquid such as a large amount of cleaning solution, buffer, luminescent solutions, testing cost is high, and entire instrument must include complicated fluid pathSystem；In addition, the sample size that single detection project needs is high, printer need to be individually connected, experiment flow is complicated, testing result instituteIt takes time longer.
Summary of the invention
The present invention is described in view of the deficienciess of the prior art, provide a kind of chemical luminescence detector and its application methodChemical luminescence detector no setting is required liquid channel system, equipment failure rate is low, safeguards more convenient；Sample needed for detection projectAmount is few, and parallel independent multiple sense channels can synchronize quantitative analysis, and the occurrence of can be avoided cross-infection；As a resultIt can directly print, detection efficiency is high, can meet the quantitative detection of fast accurate.
The technical scheme to solve the above technical problems is that a kind of chemical luminescence detector, the chemiluminescence is surveyedDetermining instrument includes bottom plate and outer cover, and the bottom plate is bolted with outer cover, the bottom plate upper end be fixedly connected with supporting vertical plate,Electrically-controlled component and printer module, the supporting vertical plate be equipped with sample process component, sample-adding needle assemblies, reagent connection needle assemblies andRead component, the sample process component, sample-adding needle assemblies, reagent connection needle assemblies, reading component respectively with the electrically-controlled componentIt is connected by winding displacement；
The one end side of the supporting vertical plate is fixedly connected with stepper motor, and the other end side of the supporting vertical plate is fixedly connectedThere is driven wheel, be socketed with stepping belt on the synchronous pulley of the stepper motor, described in the other end socket of the stepping beltDriven wheel；The supporting vertical plate side is equipped with support plate track, and sample process component side and the support plate track are logicalSliding block connection is crossed, sample process component side is fixedly connected with connecting plate, the other end of the connecting plate and the stepping beltIt is fixedly connected；The sample-adding needle assemblies are bolted with the supporting vertical plate upper end, the reagent connection needle assemblies and the supportVertical plate upper end is bolted, and reagent connection needle assemblies are equipped with magnetic suction module, the reading component in the supporting vertical plateEnd is bolted；
The sample process component includes rack for test tube, run-bake-needle fault plate, suction nozzle placement plate and raising module, and the rack for test tube rear side is solidSurely the run-bake-needle fault plate is connected, run-bake-needle fault plate lower end is fixedly connected with run-bake-needle fault box, is fixedly connected with the suction on rear side of the run-bake-needle fault plateHead placement plate, the suction nozzle placement plate includes sample suction nozzle placement plate and transfer suction nozzle placement plate, in sample suction nozzle placement plateIt is equipped with sample suction nozzle, shifts and is equipped with transfer suction nozzle in suction nozzle placement plate, be fixedly connected with the incubation on rear side of the suction nozzle placement plateModule；The raising module includes being incubated for frame, and the incubation frame lower end is fixedly connected with heating plate, and the heating plate lower end is fixedIt is connected with thermal insulation board, the incubation frame upper end is plugged with clamping stent, and the clamping stent upper end is equipped with several strip slots, the stripReagent card item is plugged in slot, the reagent strip is equipped with sample slot and several reagent troughs, and one end of the reagent strip is pastedThere is bar code, the other end of the reagent strip is equipped with reading hole, and the reagent trough upper end has sealer.
Rack for test tube is for placing blood sample to be measured；It needs to wash blood sample when detecting blood sample, react, magnetic bead is inhaledAttached to wait processing, the reagent trough on reagent strip is used to place the reagent solutions such as corresponding cleaning solution, buffer, magnetic bead and exciting liquid；Reagent strip is plugged in strip slot, more convenient to use convenient for the installation and replacement of reagent strip；The setting of heating plate is convenient forIncubation processing is carried out to blood sample to be measured, the setting of thermal insulation board can cause to avoid in the heat transfer to other component of heating plateThermal loss and damage to other component；Run-bake-needle fault plate can be suitable for sample suction nozzle simultaneously and shift the removing of suction nozzle；Sample-addingComponent lower end sample loading suction nozzle, sample-adding needle assemblies are for the blood sample to be measured on rack for test tube to be added in reagent strip；ReagentJoin needle assemblies lower end and load transfer suction nozzle, reagent joins needle assemblies and is used for sample and reagent between reagent troughs different in reagent stripThe transfer of liquid, magnetic suction module are used for the Beads enrichment in transfer suction nozzle；Component is read for the reading to the magnetic bead isolatedDetection；Printer module can will test result and directly print, and detection efficiency is high, and result speed is fast out, can meet quicklyAccurately quantitative detection.
Based on the above technical solution, the present invention can also be improved as follows:
Further, the sample-adding needle assemblies include sample-adding bracket, sample-adding transverse electric machine, sample needle and are loaded vertical motor, describedSample-adding pedestal lower end is bolted with the supporting vertical plate upper end, and one end of the sample-adding bracket is fixedly connected with the sample-adding transverse electricMachine, the other end of the sample-adding bracket are fixedly connected with driven wheel, the synchronous pulley and the driven wheel of the sample-adding transverse electric machineBetween be socketed with the horizontal belt of sample-adding；The sample-adding pedestal upper end is equipped with sample-adding traverse rod road, the sample needle and the sample-adding traverse rodRoad is connected by sliding block, is connected between sample-adding horizontal belt and the sliding block by connecting plate；It is loaded transverse electric machine and is loaded horizontal skinThe transverse shifting of sample needle is convenient in the setting of band, is suitble to add blood sample to be measured in the reagent strip of different location.
The vertical motor of sample-adding is fixedly connected with the sliding block on the sample-adding traverse rod road, is equipped with and is loaded on rear side of the sample needleVertical track, the vertical motor of sample-adding is connect with the vertical track of the sample-adding by sliding block, described to be loaded on the synchronous pulley for indulging motorIt is cased with the vertical belt of sample-adding, the both ends for being loaded vertical belt are fixedly connected with the sample needle.It is loaded vertical motor and is loaded vertical skinThe longitudinal movement of sample needle is convenient in the setting of band, consequently facilitating to the height adjustment of sample needle.
Further, sample-adding injecting motor, sample injector pipe and pressure sensor, institute are equipped with inside the sample needleIt states sample-adding injecting motor and the sample injector pipe is connected with yoke plate by screw rod, sample injector pipe lower end, which is connected with, to be addedSample injects pump seat, and sample-adding injection pump seat lower end is connected with sample-adding injection steel needle, the sample-adding injection steel needle and the pressureSensor is associated.On toward reagent strip plus when blood sample to be measured, sample-adding injection steel needle lower end, which loads, is loaded suction nozzle, is loaded suction nozzleWhen drawing blood sample to be measured, sample-adding injection steel needle is associated with pressure sensor, and the pressure value shown by pressure sensor can be withAt a distance from learning sample-adding suction nozzle between blood sample liquid level to be measured.
Further, the reagent connection needle assemblies include connection pin frame, connection needle device, connection needle motor, connection needle screw rod, upper bearing (metal)Seat, step, link block and magnetic suction module, the connection pin frame is bolted with the supporting vertical plate upper end, describedConnection needle track is equipped on front side of pin frame, the needle device is connect with the needle track by sliding block, on rear side of the pin frameIt is fixedly connected with the needle motor, needle screw rod lower end passes through the step, and needle screw rod lower end is connected withSynchronous pulley, is cased with belt between the needle motor and the synchronous pulley of needle screw rod lower end, the needle screw rod is logicalIt crosses bearing to connect with the top chock and step, the top chock and step pass through bolt and the needle plateFrame connection, is fixedly connected with optical axis, is socketed with jackshaft among the needle screw rod between the top chock and stepSeat is held, the middle bearing bracket is connect with the optical axis by bearing, and strip grooves, the connection are equipped among the pin frameBlock inserts in the strip grooves, and one end of the link block is fixedly connected with the middle bearing bracket, the other end of the link blockIt is fixedly connected with the needle device；Connection needle device can be driven to move up and down along connection needle track by connection needle motor and link block.
The magnetic suction module includes magnetic restocking, magnetic undercarriage, magnetic plate and magnetic motor, the magnetic restocking with it is describedJoin the connection of pin frame lower end rear side bolt, the lower end of the magnetic restocking is equipped with magnetic track, one end of the magnetic undercarriage withThe magnetic track is connected by sliding block, and the magnetic undercarriage passes through the pin frame, and the other end of the magnetic undercarriage is solidSurely the magnetic plate is connected, the magnetic motor is fixedly connected on the rear side of the pin frame, the shaft of the magnetic motorAcross the pin frame, the axle end of the magnetic motor is fixedly connected with the magnetic plate, if the magnetic plate is equipped withDry magnetic needle tray.When needing to separate the magnetic bead in transfer suction nozzle, magnetic motor, which is opened, pushes magnetic plate close to transferSuction nozzle, magnetic needle tray match with transfer suction nozzle, and magnetic plate has suction-operated to magnetic bead, inhale so that magnetic bead be made to be retained in transferIn head, the liquid outflow in suction nozzle is shifted, realizes the separation of magnetic bead.
Further, the connection needle device includes connection needle back plate, connection needle housing, shifts injecting motor and injection screw, describedConnection needle back plate is bolted with the needle housing, and the needle back plate is connect with the needle track by sliding block, describedConnection needle back plate upper end is fixedly connected with upper bearing (metal) plate, and lower axle board, the transfer note are fixedly connected in the middle part of the needle back plateRadio machine is fixedly connected with the lower end of the upper bearing (metal) plate, and the shaft upper end of the transfer injecting motor passes through the upper bearing (metal)Plate, the injection screw upper end pass through the upper bearing (metal) plate, and the shaft upper end of the transfer injecting motor is connected with synchronous pulley,The injection screw upper end is connected with synchronous pulley, on the synchronous pulley and the injection screw on the transfer injecting motorIt is cased with belt between synchronous pulley, is fixedly connected with optical axis between the upper bearing (metal) plate and lower axle board, in the injection screwBetween be socketed with jackshaft board, the jackshaft board is connect with the optical axis by bearing, and jackshaft board side is solidSurely it is connected with linkage pump push plate, linkage pump push plate lower end has been connected by screw to several transfer syringes, several transfer notesThe setting of emitter can carry out the transfer of multiple blood samples simultaneously, more efficient, and the transfer syringe lower end is plugged with linkage pumpVentilation block, the side of the linkage pump ventilation block and the lower end of the needle back plate are bolted, the linkage complete air parcelShaft is fixedly connected between the upper bearing (metal) plate, the shaft is connect with linkage pump push plate by bearing, describedThe lower end of dynamic ventilation block is plugged with transfer injection steel needle, and transfer injection steel needle lower end has point tough, can be used to puncture examinationSealer on agent strip.
Further, the reading component includes reading bracket, upper reading mounting plate, optics instrument bucket, lower reading mounting plate,The reading bracket is bolted with the supporting vertical plate upper end, and one end of the reading bracket is fixedly connected with reading transverse electricMachine, the shaft upper end of the reading transverse electric machine are connected with synchronous pulley, and the other end of the reading bracket is fixedly connected with drivenIt takes turns, is cased with belt between the synchronous pulley and the driven wheel on the reading transverse electric machine；The reading pedestal upper end, which is equipped with, to be readNumber traverse rod road, the upper reading mounting plate are connect with the reading traverse rod road by sliding block, the rear side of the upper reading mounting plateIt is connect between the belt by connecting plate；The optics instrument barrel side face is fixedly connected with the lower reading mounting plate, the upper readingNumber mounting plate upper end is fixedly connected with the vertical motor of reading, it is described read vertical motor by motor fishplate bar and spring fixed column with it is describedLower reading mounting plate connection, the lower reading mounting plate upper end is fixedly connected with reading head movable plate, the upper reading mounting plateSide, which is equipped with, reads vertical track, and the reading head movable plate is connect with the vertical track of the reading by sliding block, the optics instrument bucketUpper end is connect by optical fiber with the electrically-controlled component；Reading transverse electric machine reads vertical motor for driving optics instrument bucket transverse shiftingFor driving optics instrument bucket to vertically move, when carrying out detection reading, the lower end of optics instrument bucket and the reading hole phase on reagent stripIt is corresponding.
Barcode scanning head has been bolted in the vertical motor side of reading, and the lower reading mounting plate upper end passes through bolt and connectsIt is connected to barcode scanning gun shielding plate, the barcode scanning gun shielding plate is equipped with barcode scanning mouth, and the barcode scanning mouth is vertical with the position of the barcode scanning headIt is corresponding.The setting of barcode scanning head realizes the exactitude position of sample and reagent strip for scanning the bar code information on reagent strip.
Further, it is equipped with dodge gate on front side of the outer cover, is connected between the dodge gate and the outer cover by shaft.The setting of dodge gate carries out blood sample test tube, sample suction nozzle, the placement for shifting suction nozzle and reagent strip convenient for staff, using moreAdd conveniently.
Further, the outer cover upper end is fixedly connected with computer control screen, and the computer control screen passes through route and instituteState electrically-controlled component connection.The setting of computer control screen keeps staff more convenient to the operation of the chemical luminescence detector,It visualizes stronger.
Further, the outer cover side is equipped with several heat release holes, and the setting of heat release hole is convenient for surveying the chemiluminescenceDetermine the heat dissipation of instrument；The bottom plate lower end is fixedly connected with Rubber foot, and Rubber foot can play the role of buffer protection.
In addition the invention also discloses the application method of the chemical luminescence detector, include the following steps:
1) test tube for filling blood sample to be detected is placed on rack for test tube, sample suction nozzle, In is placed in sample suction nozzle placement plateIt shifts and places transfer suction nozzle in suction nozzle placement plate；
2) various cleaning solutions, buffer, magnetic bead and the excitation for blood examination are sequentially placed into the reagent trough of reagent stripLiquid, sticks the bar code of blood sample information to be detected in one end of the reagent strip, encloses sealer in reagent strip upper end, willThe reagent strip is plugged in strip slot, and clamping stent is mounted on and is incubated on frame；
3) stepper motor drives sample process component to be moved to below the reading component, described in the reading component barcode scanning readingBar code information on reagent strip checks the consistency of the reagent strip and blood sample information to be detected.If information is inconsistent,It will appear prompt information in computer control screen, prompt to be changed to the consistent blood to be measured of information；If information unanimously if continue intoRow next step；
4) stepper motor drives sample process component to be moved to reagent connection needle assemblies position, and the reagent joins needle assembliesThe reagent strip is punched, sealer is punctured；
5) stepper motor drives sample process component to be moved to the sample needle module position, and the sample-adding needle assemblies are in sampleSample loading suction nozzle at this suction nozzle placement plate, the sample-adding component draw the blood sample to be measured on rack for test tube, and the sample-adding component willThe blood sample to be measured drawn is added in the sample slot of the reagent strip, is then turned on heating plate and is incubated for blood sample to be measured；It incubatesWhile educating, the stepper motor drives sample process component mobile, and the sample-adding component is made to carry out sample suction at run-bake-needle fault plateThe removing of head, sample suction nozzle are fallen into run-bake-needle fault box, and the reagent connection needle assemblies load transfer suction nozzle at transfer suction nozzle placement plate；
6) after the completion of being incubated for, the stepper motor drives sample process component mobile, fills the sample slot face of the reagent stripHave below the reagent connection needle assemblies of transfer suction nozzle, carry out the transfer of sample, the reagent joins needle assemblies for the sample slotInterior blood sample to be measured, which is transferred in first reagent trough of the reagent strip, to be reacted, accordingly after carrying out washing treatment, thenIt is corresponding anti-that sample liquid in first reagent trough is transferred to progress in next reagent trough again by the reagent connection needle assembliesIt answers, carrying out washing treatment, the processing of blood sample to be measured is successively carried out in different reagent troughs；
7) magnetic suction module in the reagent connection needle assemblies is automatically aligned to by the experiment flow of software approach editor has drawnTransfer suction nozzle comprising magnetic bead and sample liquid realizes the separation of magnetic bead and reaction liquid, magnetic bead is finally added to readingKong Zhong；
8) stepper motor drives sample process component mobile, makes to read component described in the reading hole face of the reagent strip,Last reading process is completed, reading result prints survey report, the corresponding printing of a blood sample by printer moduleReport；
9) after the completion of reading, the stepper motor drives sample process component mobile, and the reagent connection needle assemblies are at run-bake-needle fault plateCarry out the removing of transfer suction nozzle.
The beneficial effects of the present invention are: no setting is required the liquid channel system of chemical luminescence detector described in (1), twelvefold maintenanceIt is more convenient, failure rate is effectively reduced；(2) chemical luminescence detector and application method described in, sample needed for detection projectAmount is few, more convenient to use in terms of medical treatment；(3) chemical luminescence detector described in can carry out the processing of multiple samples simultaneouslyDetection, experiment flow is simple, and detection cycle is short, and detection efficiency is higher；(4) complete in such a way that sample-adding component is disposably loadedCross contamination between different samples is avoided the occurrence of entirely, and testing result is more accurate；(5) built-in printer component is setIt sets, testing result directly prints, and detection efficiency is high, and result speed is fast out, can meet the quantitative detection of fast accurate.
Detailed description of the invention
Fig. 1 is the schematic perspective view of chemical luminescence detector described in embodiment；
Fig. 2 is the inside schematic perspective view of chemical luminescence detector described in embodiment；
Fig. 3 is the inside top figure of chemical luminescence detector described in embodiment；
Fig. 4 is the schematic perspective view of sample process component described in embodiment；
Fig. 5 is the top view of sample process component described in embodiment；
Fig. 6 is the schematic perspective view of raising module described in embodiment；
Fig. 7 is the main view of raising module described in embodiment；
Fig. 8 is the schematic perspective view of reagent strip described in embodiment；
Fig. 9 is the schematic perspective view that needle assemblies are loaded described in embodiment；
Figure 10 is the schematic perspective view that needle assemblies are loaded described in embodiment；
Figure 11 is the inside schematic perspective view that needle assemblies are loaded described in embodiment；
Figure 12 is the schematic perspective view that reagent described in embodiment joins needle assemblies；
Figure 13 is partial enlarged view at the A in Figure 12；
Figure 14 is the schematic perspective view that reagent described in embodiment joins needle assemblies；
Figure 15 is the inside schematic perspective view that reagent described in embodiment joins needle assemblies；
Figure 16 is the schematic perspective view of magnetic suction module described in embodiment；
Figure 17 is the right view of magnetic suction module described in embodiment；
Figure 18 is the schematic perspective view that component is read described in embodiment；
Figure 19 is the rearview that component is read described in embodiment；
Figure 20 is the top view that component is read described in embodiment；
In figure, 1 bottom plate, 2 outer covers, 3 supporting vertical plates, 4 electrically-controlled components, 5 printer modules, 6 stepper motors, 7 driven wheels, 8 steppingsBelt, 9 support plate tracks, 10 sliding blocks, 11 connecting plates；
12-1 rack for test tube, 12-2 run-bake-needle fault plate, 12-3 run-bake-needle fault box, 12-4 sample suction nozzle placement plate, 12-5 shift suction nozzle placement plate,12-6 is incubated for frame, 12-7 heating plate, 12-8 thermal insulation board, 12-9 clamping stent, 12-10 strip slot, 12-11 reagent strip, 12-12 sampleThis slot, 12-13 reagent trough, 12-14 bar code, 12-15 read hole；
13-1 is loaded bracket, and 13-2 is loaded transverse electric machine, 13-3 sample needle, and 13-4 is loaded vertical motor, and 13-5 is loaded horizontal belt, 13-6Sample-adding traverse rod road, 13-7 are loaded vertical track, and 13-8, which is loaded, indulges belt, 13-9 sample-adding injecting motor, 13-10 sample injector pipe,13-11 pressure sensor, 13-12 screw rod, 13-13 yoke plate, 13-14 sample-adding injection pump seat, 13-15 sample-adding injection steel needle；
14-1 joins pin frame, and 14-2 joins needle motor, and 14-3 joins needle screw rod, 14-4 top chock, 14-5 step, 14-6 connectionBlock, 14-7 join needle track, 14-8 optical axis, 14-9 middle bearing bracket, 14-10 strip grooves；14-11 magnetic restocking, under 14-12 magneticFrame, 14-13 magnetic plate, 14-14 magnetic motor, 14-15 magnetic track, 14-16 magnetic needle tray, 14-17 join needle back plate, 14-18Join needle housing, 14-19 shifts injecting motor, 14-20 injection screw, 14-21 upper bearing (metal) plate, 14-22 lower axle board, among 14-23Shaft bearing plate, 14-24 linkage pump push plate, 14-25 shift syringe, 14-26 linkage pump ventilation block, 14-27 shaft, 14-28 transferSteel needle is injected, 14-29 point is tough；
15-1 reads bracket, reads mounting plate on 15-2,15-3 optics instrument bucket, reads mounting plate under 15-4,15-5 reads transverse electricMachine, 15-6 read traverse rod road, and 15-7 reads vertical motor, 15-8 motor fishplate bar, 15-9 spring fixed column, the movement of 15-10 reading headPlate, 15-11 read vertical track, 15-12 optical fiber, 15-13 barcode scanning head, 15-14 barcode scanning gun shielding plate, 15-15 barcode scanning mouth；
16 dodge gates, 17 computer control screens, 18 heat release holes, 19 Rubber foots；A test tube, b sample suction nozzle, c shift suction nozzle.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to specific reality of the inventionThe mode of applying is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.ButThe invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to this hairSimilar improvement is done in the case where bright intension, therefore the present invention is not limited by the specific embodiments disclosed below.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the inventionThe normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description toolThe purpose of the embodiment of body, it is not intended that in the limitation present invention.
As shown, a kind of chemical luminescence detector, the chemical luminescence detector includes bottom plate 1 and outer cover 2, describedBottom plate 1 is bolted with outer cover 2, and 1 upper end of bottom plate is fixedly connected with supporting vertical plate 3, electrically-controlled component 4 and groups of printersPart 5, the supporting vertical plate 3 are equipped with sample process component, sample needle 13-3 component, reagent connection needle assemblies and reading component, instituteIt states sample process component, sample needle 13-3 component, reagent connection needle assemblies, reading component and passes through row with the electrically-controlled component 4 respectivelyLine connection；
The one end side of the supporting vertical plate 3 is fixedly connected with stepper motor 6, and the other end side of the supporting vertical plate 3 is fixedIt is connected with driven wheel 7, stepping belt 8, the other end of the stepping belt 8 are socketed on the synchronous pulley of the stepper motor 6It is socketed the driven wheel 7；3 side of supporting vertical plate is equipped with support plate track 9, sample process component side and the branchFagging track 9 is connected by sliding block 10, and sample process component side is fixedly connected with connecting plate 11, the connecting plate 11 it is anotherEnd is fixedly connected with the stepping belt 8；The sample needle 13-3 component is bolted with 3 upper end of supporting vertical plate, describedReagent connection needle assemblies are bolted with 3 upper end of supporting vertical plate, and the reagent connection needle assemblies are equipped with magnetic suction module, the readingBook component is bolted with 3 upper end of supporting vertical plate；
The sample process component includes rack for test tube 12-1, run-bake-needle fault plate 12-2, suction nozzle placement plate and raising module, the test tubeIt is fixedly connected with the run-bake-needle fault plate 12-2 on rear side of frame 12-1, the lower end run-bake-needle fault plate 12-2 is fixedly connected with run-bake-needle fault box 12-3, describedBe fixedly connected with the suction nozzle placement plate on rear side of run-bake-needle fault plate 12-2, the suction nozzle placement plate include sample suction nozzle placement plate 12-4 andSuction nozzle placement plate 12-5 is shifted, is fixedly connected with the raising module on rear side of the suction nozzle placement plate；The raising module includes incubatingFrame 12-6 is educated, the lower end the incubation frame 12-6 is fixedly connected with heating plate 12-7, and the lower end the heating plate 12-7 is fixedly connected withThe upper end thermal insulation board 12-8, the incubation frame 12-6 is plugged with clamping stent 12-9, and the upper end the clamping stent 12-9 is equipped with several stripsReagent card 12-11 is plugged in slot 12-10, the strip slot 12-10, the reagent strip 12-11 is equipped with sample slot 12-Bar code 12-14 is posted in 12 and several reagent trough 12-13, one end of the reagent strip 12-11, the reagent strip 12-11'sThe other end is equipped with reading hole 12-15, and the upper end the reagent trough 12-13 has sealer.
The sample needle 13-3 component includes sample-adding bracket 13-1, sample-adding transverse electric machine 13-2, sample needle 13-3 and sample-addingVertical motor 13-4, the sample-adding lower end bracket 13-1 are bolted with 3 upper end of supporting vertical plate, the sample-adding bracket 13-1'sOne end is fixedly connected with the sample-adding transverse electric machine 13-2, and the other end of the sample-adding bracket 13-1 is fixedly connected with driven wheel 7, describedIt is loaded between the synchronous pulley and the driven wheel 7 of transverse electric machine 13-2 and is socketed with the horizontal belt 13-5 of sample-adding；The sample-adding bracket 13-1 upper end is equipped with sample-adding traverse rod road 13-6, the sample needle 13-3 and the sample-adding traverse rod road 13-6 and is connect by sliding block 10, describedIt is loaded between horizontal belt 13-5 and the sliding block 10 and is connected by connecting plate 11；
The vertical motor 13-4 of sample-adding is fixedly connected with the sliding block 10 on the sample-adding traverse rod road 13-6, after the sample needle 13-3Side is connect with the vertical track 13-7 of the sample-adding by sliding block 10 equipped with vertical track 13-7, the vertical motor 13-4 of sample-adding is loaded, instituteState and be cased with the vertical belt road 13-8 of sample-adding on the synchronous pulley for being loaded vertical motor 13-4, the both ends for being loaded vertical belt road 13-8 withThe sample needle 13-3 is fixedly connected.
Sample-adding injecting motor 13-9, sample injector pipe 13-10 and pressure sensing are equipped with inside the sample needle 13-3Device 13-11, the sample-adding injecting motor 13-9 connect the sample injector pipe 13- with yoke plate 13-13 by screw rod 13-1210, the lower end sample injector pipe 13-10 is connected with sample-adding injection pump seat 13-14, and the sample-adding injects the lower end pump seat 13-14It is connected with sample-adding injection steel needle 13-15, the sample-adding injection steel needle 13-15 is associated with the pressure sensor 13-11.
Reagent connection needle assemblies include connection pin frame 14-1, connection needle device, connection needle motor 14-2, connection needle screw rod 14-3, onBearing block 14-4, step 14-5, link block 14-6 and magnetic suction module, the connection pin frame 14-1 and the supporting vertical plate3 upper ends are bolted, and connection needle track 14-7, the needle device and the needle track are equipped on front side of the pin frame 14-114-7 is connected by sliding block 10, is fixedly connected with the needle motor 14-2, the needle screw rod on rear side of the pin frame 14-1The lower end 14-3 passes through the step 14-5, and the lower end needle screw rod 14-3 is connected with synchronous pulley, the needle motorBelt is cased between 14-2 and the synchronous pulley of the lower end needle screw rod 14-3, the needle screw rod 14-3 passes through bearing and instituteTop chock 14-4 is stated to connect with step 14-5, the top chock 14-4 and step 14-5 by bolt with it is describedJoin pin frame 14-1 connection, optical axis 14-8 is fixedly connected between the top chock 14-4 and step 14-5, it is describedMiddle bearing bracket 14-9, the middle bearing bracket 14-9 and the optical axis 14-8 is socketed among needle screw rod 14-3 to connect by bearingIt connects, strip grooves 14-10 is equipped among the pin frame 14-1, the link block 14-6 is inserted in the strip grooves 14-10, instituteThe one end for stating link block 14-6 is fixedly connected with the middle bearing bracket 14-9, and the other end of the link block 14-6 is fixedly connected with instituteState connection needle device；
The magnetic suction module includes magnetic restocking, magnetic undercarriage 14-12, magnetic plate 14-13 and magnetic motor 14-14, the magneticFrame is sucted to connect with the lower end pin frame 14-1 rear side bolt, the lower end of the magnetic restocking is equipped with magnetic track 14-15,One end of the magnetic undercarriage 14-12 is connect with the magnetic track 14-15 by sliding block 10, and the magnetic undercarriage 14-12 is wornThe pin frame 14-1 is crossed, the other end of the magnetic undercarriage 14-12 is fixedly connected with the magnetic plate 14-13, the magneticMotor 14-14 is fixedly connected on the rear side of the pin frame 14-1, and the shaft 14-27 of the magnetic motor 14-14 passes through instituteConnection pin frame 14-1 is stated, the end shaft 14-27 of the magnetic motor 14-14 is fixedly connected with the magnetic plate 14-13, the magneticSuction disc 14-13 is equipped with several magnetic needle tray 14-16.
The connection needle device includes connection needle back plate 14-17, connection needle housing 14-18, transfer injecting motor 14-19 and injection screw14-20, the needle back plate 14-17 are bolted with the needle housing 14-18, the needle back plate 14-17 with it is describedConnection needle track 14-7 is connected by sliding block 10, and the upper end needle back plate 14-17 is fixedly connected with upper bearing (metal) plate 14-21, describedLower axle board 14-22, the transfer injecting motor 14-19 and the upper bearing (metal) plate are fixedly connected in the middle part of connection needle back plate 14-17The lower end of 14-21 is fixedly connected, and the upper end shaft 14-27 of the transfer injecting motor 14-19 passes through the upper bearing (metal) plate 14-21, the upper end the injection screw 14-20 passes through the upper bearing (metal) plate 14-21, the shaft 14- of the transfer injecting motor 14-1927 upper ends are connected with synchronous pulley, and the upper end the injection screw 14-20 is connected with synchronous pulley, the transfer injecting motor 14-Be cased with belt between the synchronous pulley on synchronous pulley and the injection screw 14-20 on 19, the upper bearing (metal) plate 14-21 andIt is fixedly connected with optical axis 14-8 between lower axle board 14-22, intermediate bearing plate 14- is socketed among the injection screw 14-2023, the jackshaft board 14-23 are connect with the optical axis 14-8 by bearing, and the side jackshaft board 14-23 is fixedIt is connected with linkage pump push plate 14-24, the linkage pump lower end push plate 14-24 has been connected by screw to several transfer syringe 14-The lower end 25, the transfer syringe 14-25 is plugged with linkage pump ventilation block 14-26, the side of the linkage pump ventilation block 14-26It is bolted with the lower end of the needle back plate 14-17, it is solid between the linkage complete air parcel and the upper bearing (metal) plate 14-21Surely it is connected with shaft 14-27, the shaft 14-27 and linkage pump push plate 14-24 to connect by bearing, the linkage ventilationThe lower end of block is plugged with transfer injection steel needle 14-28, and the transfer injection lower end steel needle 14-28 has the tough 14-29 of point.
The reading component includes reading bracket 15-1, upper reading mounting plate 15-2, optics instrument bucket 15-3, the installation of lower readingPlate 15-4, the reading bracket 15-1 are bolted with 3 upper end of supporting vertical plate, and one end of the reading bracket 15-1 is fixedIt is connected with reading transverse electric machine 15-5, the upper end shaft 14-27 of the reading transverse electric machine 15-5 is connected with synchronous pulley, the readingThe other end of bracket 15-1 is fixedly connected with driven wheel 7, synchronous pulley and the driven wheel 7 on the reading transverse electric machine 15-5Between be cased with belt；The reading upper end bracket 15-1 is equipped with reading traverse rod road 15-6, the upper reading mounting plate 15-2 and instituteIt states reading traverse rod road 15-6 to connect by sliding block 10, passes through company between the rear side and the belt of the upper reading mounting plate 15-2Plate 11 connects；The side the optics instrument bucket 15-3 is fixedly connected with the lower reading mounting plate 15-4, the upper reading mounting plate 15-2 upper ends are fixedly connected with the vertical motor 15-7 of reading, and the vertical motor 15-7 of reading passes through motor fishplate bar 15-8 and spring fixed column15-9 is connect with the lower reading mounting plate 15-4, and the upper end lower reading mounting plate 15-4 is fixedly connected with reading head movable plate15-10, the upper reading side mounting plate 15-2, which is equipped with, reads vertical track 15-11, the reading head movable plate 15-10 with it is describedIt reads vertical track 15-11 to connect by sliding block 10, the optics instrument upper end bucket 15-3 passes through optical fiber 15-12 and the electrically-controlled component4 connections；
Barcode scanning head 15-13, the upper end lower reading mounting plate 15-4 has been bolted in the vertical side motor 15-7 of readingBarcode scanning gun shielding plate 15-14 is bolted, the barcode scanning gun shielding plate 15-14 is equipped with barcode scanning mouth 15-15, the barcode scanningThe position of the mouth 15-15 and barcode scanning head 15-13 is vertically opposite to answer.
It is equipped with dodge gate 16 on front side of the outer cover 2, is connected between the dodge gate 16 and the outer cover 2 by shaft.InstituteIt states 2 upper end of outer cover and is fixedly connected with computer control screen 17, the computer control screen 17 is connected by route and the electrically-controlled component 4It connects.2 side of outer cover is equipped with several heat release holes 18, and 1 lower end of bottom plate is fixedly connected with Rubber foot 19.
The application method of the chemical luminescence detector, includes the following steps:
1) test tube for filling blood sample to be detected is placed on rack for test tube 12-1, places sample on sample suction nozzle placement plate 12-4Suction nozzle places transfer suction nozzle on transfer suction nozzle placement plate 12-5；
2) be sequentially placed into the reagent trough 12-13 of reagent strip 12-11 the various cleaning solutions for blood examination, buffer,Magnetic bead and exciting liquid stick the bar code 12-14 of blood sample information to be detected in one end of the reagent strip 12-11, in the examinationAgent strip 12-11 encloses sealer in upper end, and the reagent strip 12-11 is plugged in strip slot 12-10, clamping stent 12-9 is pacifiedOn incubation frame 12-6；
3) stepper motor 6 drives sample process component to be moved to below the reading component, and the reading component barcode scanning reads instituteThe bar code 12-14 information on reagent strip 12-11 is stated, it is consistent with blood sample information to be detected to check the reagent strip 12-11Property；
4) stepper motor 6 drives sample process component to be moved to reagent connection needle assemblies position, and the reagent joins needle groupPart punches the reagent strip 12-11, and sealer is punctured；
5) stepper motor 6 drives sample process component to be moved to the sample needle 13-3 module position, the sample needle 13-3 components sample loading suction nozzle at sample suction nozzle placement plate 12-4, the sample-adding component draw the blood to be measured on rack for test tube 12-1The blood sample to be measured of absorption is added in the sample slot 12-12 of the reagent strip 12-11, is then turned on by sample, the sample-adding componentHeating plate 12-7 is incubated for blood sample to be measured；While incubation, the stepper motor 6 drives sample process component mobile, makesThe sample-adding component carries out the removing of sample suction nozzle at run-bake-needle fault plate 12-2, and sample suction nozzle is fallen into run-bake-needle fault box 12-3, the examinationAgent joins needle assemblies and loads transfer suction nozzle at transfer suction nozzle placement plate 12-5；
6) after the completion of being incubated for, the stepper motor 6 drives sample process component mobile, makes the sample slot 12-12 of the reagent stripReagent connection needle assemblies lower section of the face equipped with transfer suction nozzle, carries out the transfer of sample, the reagent connection needle assemblies will be describedBlood sample to be measured in sample slot 12-12 is transferred in first reagent trough 12-13 of the reagent strip 12-11 and carries out accordinglyAfter reaction, carrying out washing treatment, then the sample liquid in first reagent trough 12-13 is transferred to down by the reagent connection needle assemblies againIt is reacted accordingly in one reagent trough 12-13, carrying out washing treatment, successively carries out blood to be measured in different reagent trough 12-13The processing of sample；
7) magnetic suction module in the reagent connection needle assemblies is automatically aligned to by the experiment flow of software approach editor has drawnTransfer suction nozzle comprising magnetic bead and sample liquid realizes the separation of magnetic bead and reaction liquid, magnetic bead is finally added to readingIn the 12-15 of hole；
8) stepper motor 6 drives sample process component mobile, makes reading described in the reading hole 12-15 face of the reagent stripComponent, completes last reading process, and reading result prints survey report, a blood sample corresponding one by printer module 5A printed report；
9) after the completion of reading, the stepper motor 6 drives sample process component mobile, and the reagent connection needle assemblies are in run-bake-needle fault plateThe removing of transfer suction nozzle is carried out at 12-2.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned realityIt applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not depositedIn contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneouslyIt cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the artIt says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the inventionRange.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.