Disclosure of Invention
The invention aims to provide a multifunctional stem cell culture plate, which aims to solve the problem that stem cells are easy to pollute in the prior art.
In order to achieve the above purpose, the multifunctional stem cell culture plate comprises a bottom plate, a coaming and a separation structure, wherein the coaming is fixedly connected with the bottom plate and surrounds the bottom plate, the separation structure is fixedly connected with the coaming and is positioned on the inner side of the coaming, the separation structure comprises a first plate, a second plate, a baffle plate, a spring and a pull rod, one end of the first plate is fixedly connected with the coaming and is positioned on the inner side of the coaming, the width of the first plate is equal to the width of the inner diameter of the coaming, the second plate is propped against the first plate and is positioned between the first plate and the coaming, the baffle plate is fixedly connected with the second plate and is positioned on one side of the second plate far away from the bottom plate, one end of the second plate is positioned on the other end of the second plate, the other end of the spring is fixedly connected with the coaming, passes through the coaming and is in sliding connection with the first plate, and the other end of the pull rod is positioned on the other side of the coaming.
The pull rod comprises a first rod, a second rod and a handle, one end of the first rod is fixedly connected with the second plate and is located at one end of the second plate away from the first plate, the other end of the second rod is slidably connected with the coaming and stretches out of the coaming, the second plate at one end of the second rod is fixedly connected with the second plate and is located at one end of the second plate away from the first plate, the other end of the second rod is slidably connected with the coaming and stretches out of the coaming, the spring is located between the first rod and the second rod, one end of the handle is fixedly connected with the first rod, the other end of the handle is fixedly connected with the second rod, and the handle is located at the outer side of the coaming.
The separation structure further comprises a first rubber sleeve, wherein the first rubber sleeve is fixedly connected with the first plate and is positioned between the first plate and the second plate.
The separation structure further comprises a second rubber sleeve, wherein the second rubber sleeve is fixedly connected with the second plate and is positioned between the first rubber sleeve and the second plate.
The culture plate further comprises a cover plate, wherein the cover plate is hinged with the coaming and is located on one side, far away from the bottom plate, of the separation structure.
The cover plate comprises an insulating layer, a first conductive braiding layer, a second conductive braiding layer and a power supply, wherein the insulating layer is hinged to the coaming and is located at one end of the coaming away from the bottom plate, air holes are formed in the insulating layer, the number of the air holes is multiple, the air holes are uniformly distributed in the insulating layer, the first conductive braiding layer is fixedly connected with the insulating layer and located at one side of the insulating layer, facing the separation structure, the second conductive braiding layer is fixedly connected with the insulating layer and located at one side of the insulating layer, facing away from the first conductive braiding layer, and the power supply is fixedly connected with the insulating layer and is electrically connected with the first conductive braiding layer and the second conductive braiding layer.
The coaming is provided with at least two injection holes, and the injection holes are respectively positioned at two sides of the separation structure.
The culture plate is also provided with a sliding plate, and one end of the sliding plate is fixedly connected with the first plate and is positioned between the common plate and the bottom plate.
Wherein the culture plate further comprises a switch, and the switch is hinged with the coaming and is covered by the injection hole.
The invention relates to a multifunctional stem cell culture plate, which consists of a bottom plate, a coaming and a separation structure, wherein the separation structure consists of a first plate, a second plate, a baffle plate, springs and a pull rod, a culture medium is placed in the first plate, the second plate and the baffle plate, stem cells grow in the culture medium, the first plate and the second plate are propped against each other by the elasticity of the springs, when the stem cells are required to be separated along with the growth of the stem cells, the pull rod is pulled outwards to overcome the elasticity of the springs, so that the first plate and the second plate are separated, a part of stem cells fall into the bottom plate from a gap between the first plate and the second plate, and finally the pull rod is loosened, and under the action of the elasticity of the springs, the first plate and the second plate are reset, so that the stem cells are prevented from being polluted by external devices when being separated, and the effect of stem cells is obtained.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of the structure of a culture plate of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic view of the structure at the injection hole of the invention;
FIG. 4 is a schematic view of the structure of the cover plate of the present invention;
In the figure: 100-culture plate, 10-bottom plate, 20-coaming, 21-injection hole, 30-partition structure, 31-first plate, 32-second plate, 33-baffle, 34-spring, 35-pull rod, 351-first rod, 352-second rod, 353-handle, 36-first rubber sleeve, 37-second rubber sleeve, 40-cover plate, 41-insulating layer, 411-air hole, 42-first conductive braid, 43-second conductive braid, 44-power supply, 50-slide plate, 60-switch.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
In the description of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, in the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1 to 4, the invention provides a multifunctional stem cell culture plate 100, which comprises a bottom plate 10, a surrounding plate 20 and a separation structure 30, wherein the surrounding plate 20 is fixedly connected with the bottom plate 10 and surrounds the bottom plate 10, the separation structure 30 is fixedly connected with the surrounding plate 20 and is positioned at the inner side of the surrounding plate 20, the separation structure 30 comprises a first plate 31, a second plate 32, a baffle 33, a spring 34 and a pull rod 35, one end of the first plate 31 is fixedly connected with the surrounding plate 20 and is positioned at the inner side of the surrounding plate 20, the width of the first plate 31 is equal to the width of the inner diameter of the surrounding plate 20, the second plate 32 is abutted against the first plate 31 and is positioned between the first plate 31 and the surrounding plate 20, the baffle 33 is fixedly connected with the second plate 32 and is positioned at one side of the second plate 32 far away from the bottom plate 10, one end of the second plate 32 is positioned at the other end of the second plate 32 far from the first plate 31, the spring 34 is fixedly connected with the second plate 34 and is positioned at the other end of the second plate 35 which is fixedly connected with the other end of the surrounding plate 20, and is fixedly connected with the pull rod 35.
In this embodiment, the culture plate 100 is placed in a room with a temperature suitable for stem cell growth, at the initial stage of stem cell cultivation, a culture medium is placed in a groove formed by enclosing the first plate 31, the second plate 32 and the baffle 33, the first plate 31 and the second plate 32 are kept tightly attached under the action of the elastic force of the spring 34, as the stem cells grow, the stem cells need to be partitioned after being increased in volume, at this time, an operator needs to hold the pull rod 35, overcomes the elastic force of the spring 34 and pulls the pull rod 35 outwards, the pull rod 35 drives the second plate 32 to separate from the first plate 31, a part of stem cells drop into the bottom plate 10 from a gap between the first plate 31 and the second plate 32, a part of stem cells remain on the first plate 31 and the second plate 32, the culture medium can be added according to the requirement, and finally the pull rod 35 is released, under the action of the elastic force of the spring 34, the first plate 31 and the second plate 31 are prevented from being polluted by the outside, and the stem cells are prevented from being polluted by the reset apparatus.
Further, the pull rod 35 includes a first rod 351, a second rod 352, and a handle 353, one end of the first rod 351 is fixedly connected to the second plate 32 and is located at an end of the second plate 32 away from the first plate 31, the other end of the second rod 352 is slidably connected to the shroud 20 and extends out of the shroud 20, the second plate 32 at one end of the second rod 352 is fixedly connected to the second plate 32 and is located at an end of the second plate 32 away from the first plate 31, the other end of the second rod 352 is slidably connected to the shroud 20 and extends out of the shroud 20, the spring 34 is located between the first rod 351 and the second rod 352, one end of the handle 353 is fixedly connected to the first rod 351, the other end of the handle 353 is fixedly connected to the second rod 352, and the handle 353 is located outside the shroud 20.
In this embodiment, by the arrangement of the first rod 351 and the second rod 352, the second plate 32 is more stable in tensile force, and the second plate 32 is prevented from rotating during pulling, so that better stress can be achieved by the handle 353.
Further, the separation structure 30 further includes a first rubber sleeve 36, where the first rubber sleeve 36 is fixedly connected to the first plate 31 and is located between the first plate 31 and the second plate 32. In the example of this embodiment, the first rubber sleeve 36 is clamped between the first plate 31 and the second plate 32 under the action of the elastic force of the spring 34, the first rubber sleeve 36 is pressed, and the first rubber sleeve 36 deforms to generate an elastic force for the contact ports of the first plate 31 and the second plate 32, so as to enhance the sealing performance of the contact ports of the first plate 31 and the second plate 32, and avoid the stem cells from falling from between the first plate 31 and the second plate 32 at the initial stage of culturing.
Further, the separation structure 30 further includes a second rubber sleeve 37, where the second rubber sleeve 37 is fixedly connected to the second plate 32 and is located between the first rubber sleeve 36 and the second plate 32. In the example of this embodiment, the second rubber sleeve 37 is clamped between the first rubber sleeve 36 and the second plate 32 under the action of the elastic force of the spring 34, the second rubber sleeve 37 is pressed, and the second rubber sleeve 37 deforms to generate an elastic force for the contact ports of the first rubber sleeve 36 and the second plate 32, so as to enhance the sealing performance of the contact ports of the first plate 31 and the second plate 32, and avoid leakage of the culture medium from the space between the first plate 31 and the second plate 32 at the initial stage of cultivation.
Further, the culture plate 100 further comprises a cover plate 40, wherein the cover plate 40 is hinged with the coaming 20 and is positioned on the side of the separation structure 30 away from the bottom plate 10. In the example of the present embodiment, the closing and opening of the cover 40 isolate the stem cells from the outside, so as to avoid the pollution of the stem cells by the outside air.
Further, the cover plate 40 includes an insulating layer 41, a first conductive braid 42, a second conductive braid 43 and a power supply 44, where the insulating layer 41 is hinged to the enclosing plate 20 and located at one end of the enclosing plate 20 away from the bottom plate 10, the insulating layer 41 has a plurality of air holes 411, the air holes 411 are uniformly distributed on the insulating layer 41, the first conductive braid 42 is fixedly connected with the insulating layer 41 and located at one side of the insulating layer 41 facing the separation structure 30, the second conductive braid 43 is fixedly connected with the insulating layer 41 and located at one side of the insulating layer 41 facing away from the first conductive braid 42, and the power supply 44 is fixedly connected with the insulating layer 41 and electrically connected with the first conductive braid 42 and the second conductive braid.
In this embodiment, when stem cells are cultivated and filtered and separated, the power supply 44 is started, the power supply 44 is fixedly connected with the insulating layer 41 by using a pulse voltage generator, the pulse frequency of the power supply 44 is in the range of twenty to fifty hertz during operation, the insulating layer 41 is made of organic fibers, excessive static accumulation is avoided, the thickness of the insulating layer 41 is one millimeter, the first conductive braid 42 and the second conductive braid 43 are prevented from being contacted and disconnected, the first conductive braid 42 and the second conductive braid 43 are braided by metal wires, an electrostatic field with positive outside and negative inside is formed after the first conductive braid 42 and the second conductive braid 43 are electrified, and as dust in the air is generally positively charged, the dust is prevented from entering the cavity to pollute the stem cells, bacteria generated carelessly in the cavity are negatively charged and adsorbed to the surface of the outer conductive mesh, and the like static field is prevented from entering the cavity, so that the bacteria are killed under the effect of high voltage, and the like static field is left, thereby the effect of killing the stem cells is obtained.
Further, the shroud 20 has injection holes 21, and the number of the injection holes 21 is at least two and located at two sides of the separation structure 30. In the example of this embodiment, after the stem cells are partitioned, the culture medium may be added through the two injection holes 21, so that the cover 40 is prevented from being opened when the culture medium is added, and further, contamination of the stem cells caused by bacteria in the outside air or the like is prevented from occurring when the cover 40 is opened.
Further, the culture plate 100 further has a sliding plate 50, and one end of the sliding plate 50 is fixedly connected to the first plate 31 and located between the general and the bottom plate 10. In the example of the present embodiment, the sliding plate 50 is disposed obliquely, the other end of the sliding plate 50 is located below the second plate 32, and the stem cells fall from the first plate 31 onto the sliding plate 50, and then the sliding plate 50 introduces the stem cells onto the bottom plate 10, so as to reduce the impact of stem cell division.
Further, the culture plate 100 further comprises a switch 60, and the switch 60 is hinged with the enclosing plate 20 and is shielded by the injection hole 21. In the example of the present embodiment, the switch 60 is in a circular plate shape, and when the culture medium is not required to be added, the switch 60 is attached to the shroud 20, and the switch 60 blocks the injection hole 21, thereby avoiding contamination of stem cells caused by entry of external bacteria into the shroud 20.
The above disclosure is only a preferred embodiment of the present invention, and it should be understood that the scope of the invention is not limited thereto, and those skilled in the art will appreciate that all or part of the procedures described above can be performed according to the equivalent changes of the claims, and still fall within the scope of the present invention.