CN110387313B - Magnetophoretic microfluidic chip based on bubble mixing - Google Patents

Abstract

Translated fromChinese

本发明公开了一种基于气泡混合的磁泳微流控芯片，所述磁泳微流控芯片包括生物样本进液口、多种检测试剂进液口、混合废气出口、过滤后的洁净气体入口、过程废液出口、最终产物萃取生物样本出口、气泡破灭微结构、上部密封胶塞和混合工作液腔。本发明通过气泡混合方式，利用在芯片底部通入过滤后的洁净气体，在混合工作液腔内试剂中产生气泡，利用气泡自身浮力，使气泡在试剂中不断上升扰动周围流体产生微涡旋，进而达到使混合工作液腔内试剂和磁珠充分混合的效果。本发明解决了微小腔体内生物样本液样本和萃取磁珠混合不均匀的问题，适用于微流控生物样本提取及试剂混合领域。

The invention discloses a magnetophoresis microfluidic chip based on bubble mixing. The magnetophoresis microfluidic chip includes a biological sample liquid inlet, a liquid inlet for various detection reagents, a mixed waste gas outlet, and a filtered clean gas inlet. , process waste liquid outlet, final product extraction biological sample outlet, bubble burst microstructure, upper sealant plug and mixed working fluid chamber. The invention uses the bubble mixing method to generate bubbles in the reagent in the mixed working fluid cavity by using the filtered clean gas at the bottom of the chip, and uses the buoyancy of the bubbles to make the bubbles continuously rise in the reagent and disturb the surrounding fluid to generate micro-vortexes. Then, the effect of fully mixing the reagents and the magnetic beads in the mixed working fluid chamber is achieved. The invention solves the problem of uneven mixing of the biological sample liquid sample and the extraction magnetic beads in the tiny cavity, and is suitable for the fields of microfluidic biological sample extraction and reagent mixing.

Description

Translated fromChinese

一种基于气泡混合的磁泳微流控芯片A Magnetophoretic Microfluidic Chip Based on Bubble Mixing

技术领域technical field

本发明属于微流控相关技术领域，涉及一种微流控生物样本提取及试剂混合装置。The invention belongs to the technical field of microfluidics, and relates to a microfluidic biological sample extraction and reagent mixing device.

背景技术Background technique

在基因分析和疾病检测中，常常需要DNA提取、扩增和分离等过程，其中DNA提取是最为关键的步骤。传统人工核算提取方法操作繁琐，浪费大量人力，而且对试剂消耗很大，每个步骤没有精确的控制，难以推广到大规模重复实验，所以需要将提取过程和微流控芯片结合实现微全分析系统。In gene analysis and disease detection, processes such as DNA extraction, amplification and isolation are often required, of which DNA extraction is the most critical step. The traditional manual accounting extraction method is cumbersome, wastes a lot of manpower, and consumes a lot of reagents. There is no precise control of each step. system.

微全分析系统具备在芯片实验室上实现过程集成化、自动化、微缩化的特点，能够极大地减少试剂的消耗量，缩短分析时间，提高分析检测效率。因此在疾病诊断、生化分析、临床实验领域获得了广泛的关注。The micro-total analysis system has the characteristics of realizing process integration, automation and miniaturization on the chip laboratory, which can greatly reduce the consumption of reagents, shorten the analysis time, and improve the efficiency of analysis and detection. Therefore, it has received extensive attention in the fields of disease diagnosis, biochemical analysis, and clinical experiments.

发明内容SUMMARY OF THE INVENTION

为了解决现有微量萃取和生物样品提取装置操作复杂，难以标准化自动化，需要大量人力时间重复实验，设备集成度小，响应时间长和价格昂贵的技术问题，本发明提供了一种基于气泡混合的磁泳微流控芯片。本发明通过气泡混合方式，利用在芯片底部通入过滤后的洁净气体，在混合工作液腔内试剂中产生气泡，利用气泡自身浮力，使气泡在试剂中不断上升扰动周围流体产生微涡旋，进而达到使混合工作液腔内试剂和磁珠充分混合的效果。In order to solve the technical problems of complex operation of the existing micro-extraction and biological sample extraction devices, difficulty in standardization and automation, requiring a lot of manpower and time to repeat experiments, low equipment integration, long response time and high price, the present invention provides a bubble mixing-based technology. Magnetophoretic microfluidic chip. The invention adopts the bubble mixing method, utilizes the filtered clean gas at the bottom of the chip to generate bubbles in the reagent in the mixed working fluid chamber, and utilizes the buoyancy of the bubbles to make the bubbles continuously rise in the reagent and disturb the surrounding fluid to generate micro-vortexes. Then, the effect of fully mixing the reagents and the magnetic beads in the mixed working fluid chamber is achieved.

本发明的目的是通过以下技术方案实现的：The purpose of this invention is to realize through the following technical solutions:

一种基于气泡混合的磁泳微流控芯片，包括生物样本进液口、多种检测试剂进液口、混合废气出口、过滤后的洁净气体入口、过程废液出口、最终产物萃取生物样本出口、气泡破灭微结构、上部密封胶塞和混合工作液腔，其中：A magnetophoretic microfluidic chip based on bubble mixing, including a biological sample liquid inlet, a variety of detection reagent liquid inlets, a mixed exhaust gas outlet, a filtered clean gas inlet, a process waste liquid outlet, and a final product extraction biological sample outlet. , bubble burst microstructure, upper sealant plug and mixed working fluid chamber, where:

所述生物样本进液口、多种检测试剂进液口与混合工作液腔的上部相连；The biological sample liquid inlet and the various detection reagent liquid inlets are connected to the upper part of the mixed working liquid chamber;

所述混合工作液腔的上端设置有上部密封胶塞，内部设置有气泡破灭微结构；The upper end of the mixed working fluid chamber is provided with an upper sealing rubber plug, and a bubble burst microstructure is provided inside;

所述过程废液出口、最终产物萃取生物样本出口与混合工作液腔的底部相连；The process waste liquid outlet and the final product extraction biological sample outlet are connected to the bottom of the mixed working liquid chamber;

所述过滤后的洁净气体入口与混合工作液腔的底部正中位置相连，过滤后的洁净气体从混合工作液腔的底部通入溶液形成气泡；The filtered clean gas inlet is connected to the bottom center position of the mixed working fluid cavity, and the filtered clean gas is fed into the solution from the bottom of the mixed working fluid cavity to form bubbles;

所述混合废气出口与混合工作液腔的顶端相连，导出芯片内混合后的气体。The mixed exhaust gas outlet is connected to the top of the mixed working fluid chamber, and the mixed gas in the chip is led out.

工作原理：working principle:

利用在芯片底部通入过滤后的洁净气体，在混合工作液腔内试剂中产生气泡，在不引入外界干扰的情况下，利用气泡自身浮力上升扰动周围流体产生微涡旋，进而达到混合工作液腔内试剂充分混合的效果。气泡由连接于混合工作液腔的底部位于底部正中位置的过滤后的洁净气体入口通入过滤后的洁净气体产生。经过混合工作液腔内部的溶液利用气泡自身浮力上升扰动周围流体产生微涡旋，进而达到充分混合后，由混合液腔内部左中右三个倒置的尖锐三角形微结构组成的气泡破灭微结构主动消灭后，废气由连接于液腔顶端的芯片内混合后的混合废气出口排出。本发明能够将繁琐的磁珠萃取生物样本的过程在此芯片中完成，且混合效果较好。Using the filtered clean gas at the bottom of the chip, bubbles are generated in the reagents in the mixed working fluid chamber. Without introducing external interference, the bubbles themselves are used to rise and disturb the surrounding fluid to generate micro-vortexes, and then the mixed working fluid is achieved. The effect of thorough mixing of reagents in the cavity. The air bubbles are generated by passing the filtered clean gas through the filtered clean gas inlet connected to the bottom of the mixed working fluid chamber at the middle position of the bottom. After the solution inside the mixed working liquid chamber uses the bubble's own buoyancy to rise to disturb the surrounding fluid to generate a micro-vortex, and then achieve full mixing, the bubble burst microstructure composed of three inverted sharp triangular microstructures in the left, middle and right inside the mixed liquid chamber actively After elimination, the exhaust gas is discharged from the mixed exhaust gas outlet in the chip connected to the top of the liquid chamber. The invention can complete the tedious process of extracting biological samples with magnetic beads in the chip, and the mixing effect is good.

利用气泡自身浮力上升扰动周围流体产生微涡旋，进而达到充分混合的效果。The bubble's own buoyancy is used to disturb the surrounding fluid to generate micro-vortexes, thereby achieving the effect of full mixing.

相比于现有技术，本发明具有如下优点：Compared with the prior art, the present invention has the following advantages:

1、本发明能够将磁珠萃取生物样本的过程在芯片中完成，且混合效率高，无需外部设备，实现了微量萃取和生物样品提取装置的微型化、集成化和自动化。1. The present invention can complete the process of extracting biological samples by magnetic beads in a chip, and has high mixing efficiency, no external equipment is required, and miniaturization, integration and automation of micro-extraction and biological sample extraction devices are realized.

2、本发明解决了微小腔体内生物样本溶液和萃取磁珠混合不均匀的问题，适用于微流控生物样本提取及试剂混合领域。2. The invention solves the problem of uneven mixing of the biological sample solution and the extraction magnetic beads in the tiny cavity, and is suitable for the fields of microfluidic biological sample extraction and reagent mixing.

3、本发明的磁泳微流控芯片工作原理简单，利用在芯片底部通入过滤后的洁净气体，在混合工作液腔内试剂中产生气泡，在不引入外界干扰的情况下，利用气泡自身浮力上升扰动周围流体产生微涡旋，进而达到混合工作液腔内试剂充分混合的效果。3. The working principle of the magnetophoretic microfluidic chip of the present invention is simple. The filtered clean gas is introduced into the bottom of the chip to generate bubbles in the reagents in the mixed working fluid chamber. Without introducing external interference, the bubbles themselves are used. The rise of buoyancy disturbs the surrounding fluid to generate micro-vortex, thereby achieving the effect of fully mixing the reagents in the mixed working fluid chamber.

4、本发明的磁泳微流控芯片由PMMA材料使用微铣床加工成型，易于制作加工，且PMMA对环境友好，对人体无害。4. The magnetophoretic microfluidic chip of the present invention is formed from PMMA material using a micro-milling machine, which is easy to manufacture and process, and PMMA is environmentally friendly and harmless to human body.

5、本发明能够将繁琐的磁珠萃取生物样本的过程在磁泳微流控芯片中完成，且混合效果较好，为下一步萃取技术的自动化提供基础。5. The present invention can complete the tedious process of extracting biological samples with magnetic beads in a magnetophoretic microfluidic chip, and the mixing effect is good, which provides a basis for the automation of the next extraction technology.

6、本发明的磁泳微流控芯片反应步骤标准化时间较短，容易加工，价格低廉，同时，运用过滤后的洁净气体生成气泡达到混合效果，对PCR反应过程中并没有引入其它干扰。6. The reaction steps of the magnetophoretic microfluidic chip of the present invention have short standardized time, easy processing and low price. At the same time, the filtered clean gas is used to generate bubbles to achieve the mixing effect, and no other interference is introduced in the PCR reaction process.

7、本发明的磁泳微流控芯片由于组成部分为带有微结构的多层组合，可以针对不同密度粘度液体设计不同微结构和微腔室，同时采用不同压力的气体产生气泡，可以实现多种不相溶液体的乳化混合，使混合效果更好。7. Since the components of the magnetophoretic microfluidic chip of the present invention are multi-layer combinations with microstructures, different microstructures and microchambers can be designed for liquids with different densities and viscosities. The emulsification and mixing of various immiscible liquids make the mixing effect better.

附图说明Description of drawings

图1为本发明的基于气泡混合的磁泳微流控芯片的结构示意图。FIG. 1 is a schematic structural diagram of a magnetophoretic microfluidic chip based on bubble mixing of the present invention.

图2为本发明基于气泡混合的磁泳微流控芯片的立体结构示意图。FIG. 2 is a schematic three-dimensional structure diagram of a magnetophoretic microfluidic chip based on bubble mixing according to the present invention.

具体实施方式Detailed ways

下面结合附图对本发明的技术方案作进一步的说明，但并不局限于此，凡是对本发明技术方案进行修改或者等同替换，而不脱离本发明技术方案的精神和范围，均应涵盖在本发明的保护范围中。The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings, but are not limited thereto. Any modification or equivalent replacement of the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention shall be included in the present invention. within the scope of protection.

本发明提供了一种基于气泡混合的磁泳微流控芯片，如图1和2所示，所述磁泳微流控芯片由生物样本进液口1、多种检测试剂进液口、混合废气出口5、过滤后的洁净气体入口6、过程废液出口7、最终产物萃取生物样本出口8、气泡破灭微结构9、上部密封胶塞10、混合工作液腔11组成，其中：The present invention provides a magnetophoretic microfluidic chip based on bubble mixing, as shown in Figures 1 and 2, the magnetophoretic microfluidic chip consists of a biological sampleliquid inlet 1, a variety of detection reagent liquid inlets, a mixing Theexhaust gas outlet 5, the filteredclean gas inlet 6, the processwaste liquid outlet 7, the final product extractionbiological sample outlet 8, thebubble burst microstructure 9, the uppersealing rubber plug 10, and the mixed workingliquid chamber 11 are composed of:

所述多种检测试剂进液口由2号检测试剂进液口2、3号检测试剂进液口3、4号检测试剂进液口4依次排列组成；The multiple detection reagent liquid inlets are composed of No. 2 detection reagentliquid inlet 2, No. 3 detection reagentliquid inlet 3, and No. 4 detection reagentliquid inlet 4 arranged in sequence;

所述混合工作液腔11由下部半圆微腔和上部矩形微腔组成；The mixed workingfluid cavity 11 is composed of a lower semicircular microcavity and an upper rectangular microcavity;

所述混合工作液腔11上部的矩形微腔顶部设置有上部密封胶塞10；An upper sealingrubber plug 10 is arranged on the top of the rectangular micro-chamber on the upper part of the mixed workingfluid chamber 11;

所述气泡破灭微结构9由左中右三个倒置的尖锐三角形微结构组成，起到主动破碎形成的大量气泡的作用，防止泡沫溢出；Thebubble bursting microstructure 9 is composed of three inverted sharp triangular microstructures on the left, middle and right, and plays the role of actively breaking a large number of bubbles formed to prevent the foam from overflowing;

所述生物样本进液口1、2号检测试剂进液口2、3号检测试剂进液口3、4号检测试剂进液口4与混合工作液腔11上部的矩形微腔相连；The biological sampleliquid inlet 1, the No. 2 detection reagentliquid inlet 2, the No. 3 detection reagentliquid inlet 3, and the No. 4 detection reagentliquid inlet 4 are connected to the rectangular microcavity on the upper part of the mixed workingliquid cavity 11;

所述混合工作液腔11上部的矩形微腔内部设置有气泡破灭微结构9；Abubble bursting microstructure 9 is arranged inside the rectangular microcavity on the upper part of the mixed workingfluid cavity 11;

所述过程废液出口7、最终产物萃取生物样本出口8与混合工作液腔11下部的半圆微腔的底部中心相连；The processwaste liquid outlet 7 and the final product extractionbiological sample outlet 8 are connected to the bottom center of the semicircular micro-cavity at the lower part of the mixed workingliquid cavity 11;

所述过滤后的洁净气体入口6与混合工作液腔11下部的半圆微腔的底部正中位置相连，过滤后的洁净气体从混合工作液腔11下部的半圆微腔的底部通入溶液形成气泡；The filteredclean gas inlet 6 is connected to the bottom center position of the semicircular microcavity at the lower part of the mixed workingfluid cavity 11, and the filtered clean gas is fed into the solution from the bottom of the semicircular microcavity at the lower part of the mixed workingfluid cavity 11 to form bubbles;

所述混合废气出口5与混合工作液腔11上部的矩形微腔顶端相连，导出芯片内混合后的气体。The mixedexhaust gas outlet 5 is connected to the top of the rectangular microcavity on the upper part of the mixed workingfluid cavity 11, and the mixed gas in the chip is led out.

上述基于气泡混合的磁泳微流控芯片的具体工作流程如下：The specific workflow of the above-mentioned magnetophoretic microfluidic chip based on bubble mixing is as follows:

步骤1、在混合废气出口5插入钢针，使其与外界相连，保持气压平衡；Step 1. Insert a steel needle into the mixedexhaust gas outlet 5 to connect it with the outside world to maintain air pressure balance;

步骤2、将生物样本溶解在一定量溶液A中，由生物样本进液口1注射至混合工作液腔11中；Step 2. Dissolve the biological sample in a certain amount of solution A, and inject it into the mixed workingfluid chamber 11 from the biological sampleliquid inlet 1;

步骤3、由2号检测试剂进液口2注射至混合工作液腔11中一定量溶液B，3号检测试剂进液口3注射至混合工作液腔11中一定量溶液C，一定量溶液D，4号检测试剂进液口4注射至混合工作液腔11中一定量磁珠混合液；Step 3, inject a certain amount of solution B into the mixed workingfluid chamber 11 from the No. 2 detectionreagent liquid inlet 2, and inject a certain amount of solution C and a certain amount of solution D into the mixed workingliquid cavity 11 through the No. 3 detectionreagent liquid inlet 3 , the No. 4detection reagent inlet 4 is injected into a certain amount of magnetic bead mixture in the mixed workingfluid chamber 11;

步骤4、在过滤后的洁净气体入口6插入钢针，缓慢打开经精密气压控制气源通入过滤后的洁净气体直至产生气泡达到混合效果，完成初次混合，使磁珠完成对生物样本的吸附；Step 4. Insert a steel needle into the filteredclean gas inlet 6, slowly open the air source controlled by precise air pressure, and pass in the filtered clean gas until bubbles are generated to achieve the mixing effect, complete the initial mixing, and allow the magnetic beads to complete the adsorption of biological samples ;

步骤5、打开芯片反馈数控加热装置，将混合工作液腔11内的溶液加热至50℃（±5℃）并间隔通入气泡，使其温度场分布混合均匀，此过程持续15min；Step 5. Turn on the chip feedback numerical control heating device, heat the solution in the mixing workingfluid chamber 11 to 50°C (±5°C) and inject bubbles at intervals to make the temperature field distribution and mixing evenly, this process lasts for 15 minutes;

步骤6、用数控机械手将直径10mm、厚度5mm的圆柱形强化磁铁贴于芯片后表面，并沿混合工作液腔11内部做扫描状移动，通过磁力将带有生物样本的磁珠吸附固定于混合工作液腔11后壁上，此过程需要30s能完成充分吸附；Step 6. Use a numerical control manipulator to stick a cylindrical reinforced magnet with a diameter of 10mm and a thickness of 5mm on the back surface of the chip, and move it in a scanning manner along the inside of the mixing workingfluid chamber 11, and attach the magnetic beads with biological samples to the mixing chamber by magnetic force. On the back wall of the workingfluid chamber 11, this process takes 30s to complete the full adsorption;

步骤7、待磁珠吸附过程完成后将钢针插入过程废液出口7，将完成反应后的废液完全排出芯片后，从过程废液出口7拔出钢针；Step 7. After the magnetic bead adsorption process is completed, insert the steel needle into the process wasteliquid outlet 7, and after the waste liquid after the reaction is completely discharged from the chip, pull out the steel needle from the process wasteliquid outlet 7;

步骤8、由4号检测试剂进液口4注射至混合工作液腔11中一定量清洗液1，去掉磁铁将磁珠重新悬浮在溶液中并通过过滤后的洁净气体入口6插入钢针，缓慢打开经精密气压控制气源通入过滤后的洁净气体使液腔内部溶液混合均匀，完成清洗步骤一。待清洗过程完成后，用数控机械手将直径10mm、厚度5mm的圆柱形强化磁铁贴于芯片后表面，并沿混合工作液腔11内部做扫描状移动，通过磁力将带有生物生物样本的磁珠吸附固定于混合工作液腔11后壁上，此过程需要30s能完成充分吸附。待磁珠吸附过程完成后将钢针插入过程废液出口7，将完成反应后的废液完全排出芯片后，从过程废液出口7拔出钢针；Step 8. Inject a certain amount of cleaningsolution 1 into the mixed workingfluid chamber 11 from the No. 4detection reagent inlet 4, remove the magnet, resuspend the magnetic beads in the solution, and insert the steel needle through the filteredclean gas inlet 6, slowly Turn on the air source controlled by the precise air pressure and let in the filtered clean gas to mix the solution in the liquid chamber evenly, and complete thecleaning step 1. After the cleaning process is completed, a 10mm diameter and 5mm thick cylindrical reinforced magnet is attached to the rear surface of the chip with a numerical control manipulator, and moves in a scanning manner along the inside of the mixed workingfluid chamber 11, and the magnetic beads with biological samples are moved by magnetic force. The adsorption is fixed on the rear wall of the mixed workingfluid chamber 11, and this process takes 30s to complete the full adsorption. After the magnetic bead adsorption process is completed, insert the steel needle into the process wasteliquid outlet 7, and after the waste liquid after the reaction is completely discharged from the chip, pull out the steel needle from the process wasteliquid outlet 7;

步骤9、由4号检测试剂进液口4注射至混合工作液腔11中一定量清洗液2，重复步骤8的过程；Step 9, inject a certain amount of cleaningsolution 2 into the mixed workingfluid chamber 11 from the No. 4 detectionreagent liquid inlet 4, and repeat the process ofStep 8;

步骤10、移除强化磁铁并由4号检测试剂进液口4注射至混合工作液腔11中一定量溶液E，通过过滤后的洁净气体入口6插入钢针，缓慢打开经精密气压控制气源通入过滤后的洁净气体使液腔内部溶液混合均匀；Step 10. Remove the reinforced magnet and inject a certain amount of solution E into the mixed workingfluid chamber 11 from the No. 4 detectionreagent liquid inlet 4, insert the steel needle through the filteredclean gas inlet 6, and slowly open the gas source controlled by the precise air pressure The filtered clean gas is introduced to mix the solution in the liquid chamber evenly;

步骤11、将芯片放入离心机，使最后微量生物样本聚集于芯片下端后通过强力磁铁将磁珠吸附固定于混合工作液腔11后壁上，吸附完成后通过最终产物萃取生物样本出口8插入钢针抽出微量生物样本萃取液一定量。Step 11. Put the chip into the centrifuge, so that the last trace biological samples are gathered at the lower end of the chip, and then the magnetic beads are adsorbed and fixed on the rear wall of the mixed workingfluid chamber 11 by a strong magnet. After the adsorption is completed, it is inserted through the final product extractionbiological sample outlet 8 The steel needle draws out a certain amount of the microbiological sample extract.

本发明中，磁泳微流控芯片的加工材料为聚二甲基硅氧烷、聚甲基丙烯酸甲酯、玻璃和高分子树脂中的一种或其中几种的组合。In the present invention, the processing material of the magnetophoretic microfluidic chip is one or a combination of several of polydimethylsiloxane, polymethyl methacrylate, glass and polymer resin.

本发明中，产生气泡装置采用的是小气泵压缩过滤后的洁净气体而非压力气瓶产生。In the present invention, the bubble generating device adopts the clean gas compressed and filtered by a small air pump instead of a pressure gas cylinder.

本发明中，混合液腔内的溶液不只是磁珠和生物样本试剂，而是还可以是其它化学试剂，用于其它化学反应的混合和萃取等操作。In the present invention, the solution in the mixed liquid chamber is not only magnetic beads and biological sample reagents, but also other chemical reagents, which are used for operations such as mixing and extraction of other chemical reactions.

本发明中，生物样本进液口1、2号检测试剂进液口2、3号检测试剂进液口3、4号检测试剂进液口4、过程废液出口7、最终产物萃取生物样本出口8均由环保橡胶密封塞覆盖，保证液体不会泄露。In the present invention, biological sampleliquid inlet 1, No. 2 detectionreagent liquid inlet 2, No. 3 detectionreagent liquid inlet 3, No. 4 detectionreagent liquid inlet 4, process wasteliquid outlet 7, final product extractionbiological sample outlet 8 are covered by environmentally friendly rubber sealing plugs to ensure that the liquid will not leak.

Claims (5)

Translated fromChinese

1.一种基于气泡混合的磁泳微流控芯片，其特征在于所述磁泳微流控芯片包括生物样本进液口、多种检测试剂进液口、混合废气出口、过滤后的洁净气体入口、过程废液出口、最终产物萃取生物样本出口、气泡破灭微结构、上部密封胶塞和混合工作液腔，其中：1. a magnetophoretic microfluidic chip based on bubble mixing, it is characterized in that described magnetophoretic microfluidic chip comprises biological sample liquid inlet, multiple detection reagent liquid inlet, mixed waste gas outlet, filtered clean gas Inlet, process waste outlet, final product extraction biological sample outlet, bubble burst microstructure, upper sealant plug, and mixed working fluid chamber, where:所述生物样本进液口、多种检测试剂进液口与混合工作液腔的上部相连；The biological sample liquid inlet and the various detection reagent liquid inlets are connected to the upper part of the mixed working liquid chamber;所述混合工作液腔的上端设置有上部密封胶塞，内部设置有气泡破灭微结构；The upper end of the mixed working fluid chamber is provided with an upper sealing rubber plug, and a bubble burst microstructure is provided inside;所述气泡破灭微结构由左中右三个倒置的尖锐三角形微结构组成；The bubble burst microstructure is composed of three inverted sharp triangular microstructures on the left, middle and right;所述过程废液出口、最终产物萃取生物样本出口与混合工作液腔的底部相连；The process waste liquid outlet and the final product extraction biological sample outlet are connected to the bottom of the mixed working liquid chamber;所述过滤后的洁净气体入口与混合工作液腔的底部正中位置相连，过滤后的洁净气体从混合工作液腔的底部通入溶液形成气泡；The filtered clean gas inlet is connected to the bottom center position of the mixed working fluid cavity, and the filtered clean gas is fed into the solution from the bottom of the mixed working fluid cavity to form bubbles;所述混合废气出口与混合工作液腔的顶端相连，导出芯片内混合后的气体。The mixed exhaust gas outlet is connected to the top of the mixed working fluid chamber, and the mixed gas in the chip is led out.2.根据权利要求1所述的基于气泡混合的磁泳微流控芯片，其特征在于所述多种检测试剂进液口由2号检测试剂进液口、3号检测试剂进液口、4号检测试剂进液口依次排列组成。2. The magnetophoretic microfluidic chip based on bubble mixing according to claim 1, wherein the multiple detection reagent liquid inlets are composed of No. 2 detection reagent liquid inlet, No. 3 detection reagent liquid inlet, 4 The liquid inlets of the detection reagents are arranged in sequence.3.根据权利要求1或2所述的基于气泡混合的磁泳微流控芯片，其特征在于所述生物样本进液口、多种检测试剂进液口、过程废液出口、最终产物萃取生物样本出口均由环保橡胶密封塞覆盖。3. The magnetophoretic microfluidic chip based on bubble mixing according to claim 1 or 2, characterized in that the biological sample liquid inlet, various detection reagent liquid inlets, process waste liquid outlet, final product extraction biological Sample outlets are covered by environmentally friendly rubber sealing plugs.4.根据权利要求1所述的基于气泡混合的磁泳微流控芯片，其特征在于所述混合工作液腔由下部半圆微腔和上部矩形微腔组成。4 . The magnetophoretic microfluidic chip based on bubble mixing according to claim 1 , wherein the mixed working fluid cavity is composed of a lower semicircular microcavity and an upper rectangular microcavity. 5 .5.根据权利要求1所述的基于气泡混合的磁泳微流控芯片，其特征在于所述磁泳微流控芯片的加工材料为聚二甲基硅氧烷、聚甲基丙烯酸甲酯、玻璃和高分子树脂中的一种或其中几种的组合。5. The magnetophoretic microfluidic chip based on bubble mixing according to claim 1, wherein the processing material of the magnetophoretic microfluidic chip is polydimethylsiloxane, polymethyl methacrylate, One or a combination of glass and polymer resin.

Images