技术领域technical field
本发明属于焦化废水处理领域，尤其涉及一种适合处理焦化废水的连续生物强化工艺。The invention belongs to the field of coking wastewater treatment, and in particular relates to a continuous biointensification process suitable for treating coking wastewater.
背景技术Background technique
焦化废水成分复杂，有毒有害难降解有机物含量高,氨氮浓度高，焦化厂产生废水的数量与性质，根据所用工艺和化工产品精加工深度的不同而有差异水质水量变化较大，这使得焦化废水成为一种较难生物处理的废水。The composition of coking wastewater is complex, with high content of toxic, harmful and refractory organic matter, and high concentration of ammonia nitrogen. The quantity and nature of wastewater produced by coking plants vary according to the process used and the depth of chemical product finishing. The water quality and quantity vary greatly, which makes coking wastewater become a more difficult biological treatment of wastewater.
生物处理法以废水中污染物为营养源，可以在单一系统内有效去除多种污染物，相对于其他处理方法，不仅经济合理，而且操作简便，目前已作为大多数焦化厂废水处理单元的主体方法。各大焦化厂废水处理站生化处理单元大多采用以硝化-反硝化为核心的生物脱氮工艺，其中A/O、O/A/O、A/O/O、A/A/O/O等工艺方法则是目前应用较广的降低COD、脱氮工艺。当废水中有毒物质含量较高时，可导致一般生化系统中的菌体因生长代谢活动受抑制而死亡，不仅使菌体的降解速率降低，而且需要较长时间才能适应，最终会导致出水不能达标排放。通过投加高效菌可以强化系统对废水中难降解污染物的去除效果，并且还能提升系统的整体稳定性。The biological treatment method uses the pollutants in the wastewater as the nutrient source, and can effectively remove a variety of pollutants in a single system. Compared with other treatment methods, it is not only economical and reasonable, but also easy to operate. It has been used as the main body of most coking plant wastewater treatment units. method. Most of the biochemical treatment units of the wastewater treatment stations of major coking plants adopt the biological denitrification process with nitrification-denitrification as the core, of which A/O, O/A/O, A/O/O, A/A/O/O, etc. The process method is the COD reduction and denitrification process widely used at present. When the content of toxic substances in the wastewater is high, it can lead to the death of bacteria in the general biochemical system due to the inhibition of growth and metabolic activities, which not only reduces the degradation rate of the bacteria, but also takes a long time to adapt, and eventually leads to the failure of the effluent Discharge. By adding high-efficiency bacteria, the removal effect of the system on refractory pollutants in wastewater can be enhanced, and the overall stability of the system can also be improved.
焦化废水处理实际应用过程中，虽然高效菌加入原有生态系统后，可以有效提高系统的生物降解能力，但其处理能力经常具有短暂性和不稳定性。In the actual application process of coking wastewater treatment, although the high-efficiency bacteria can effectively improve the biodegradation capacity of the system after being added to the original ecosystem, its treatment capacity is often transient and unstable.
通过查新，可以检索到一些相关专利。专利(公开号CN1389408A)“用于污水和废水处理的生物反应器”，该反应器能够同时搅拌和供氧，包括一个培养罐，在其中进行微生物培养，一个供料器，用于向培养罐中提供培养液以及有机无机的培养基。该发明可以通过从外部将用于培养的溶液引入，可以使微生物易于培养。该装置对微生物培养种类不全。效率不够高。Through the novelty search, some related patents can be retrieved. Patent (Publication No. CN1389408A) "Bioreactor for Sewage and Wastewater Treatment", which can simultaneously stir and supply oxygen, including a culture tank in which microorganisms are cultivated, and a feeder for feeding the culture tank Provides culture medium and organic and inorganic medium. This invention can facilitate the cultivation of microorganisms by introducing a solution for cultivation from the outside. This device does not have all kinds of microorganisms. Not efficient enough.
中国专利“一种生物增效菌剂及其应用”(公开号CN103897997A)，该发明涉及一种生物增效菌剂该菌剂由在不同工艺中筛选到的6株高能力降解菌种按比例混合，离心后与无机盐培养液稀释菌体而成，可以降低生化出水COD浓度。该专利加入的菌剂在新环境中存活困难，很容易产生波动。Chinese patent "a biosynthetic bacterial agent and its application" (publication number CN103897997A), the invention relates to a biological synergistic bacterial agent. The bacterial agent is proportionally composed of 6 strains of high-capacity degradation bacteria screened in different processes Mixed, centrifuged and diluted with inorganic salt culture medium to reduce the COD concentration of biochemical effluent. The bacterium agent that this patent adds is difficult to survive in the new environment, is easy to produce fluctuation.
中国专利“一种生物强化装置”(公开号CN204454730U)公开了一种生物强化装置，包括污水输送管、厌氧培菌罐和好氧培菌罐，通过培养厌氧菌与好氧菌对污水处理，去除COD与氨氮。该工艺培养的菌剂容易被污染，生产量少，增效效率低。Chinese patent "a bioaugmentation device" (publication number CN204454730U) discloses a bioaugmentation device, including sewage delivery pipes, anaerobic culture tanks and aerobic culture tanks, by cultivating anaerobic bacteria and aerobic bacteria on sewage Treatment to remove COD and ammonia nitrogen. The bacteria agent cultivated by this process is easily polluted, the production volume is small, and the synergistic efficiency is low.
综上所述，目前的生物强化技术主要的目标是提高COD和氨氮的处理能力，忽视了添加菌剂在新环境的存活能力，针对焦化废水的高毒性，高波动性，外界菌种很难在活性污泥中稳定存活，因此需要开发一种高效并且长效的生物强化技术。To sum up, the main goal of the current bioaugmentation technology is to improve the treatment capacity of COD and ammonia nitrogen, ignoring the survivability of the added bacteria in the new environment. For the high toxicity and high volatility of coking wastewater, it is difficult for external bacteria to It survives stably in activated sludge, so it is necessary to develop an efficient and long-lasting bioaugmentation technology.
发明内容Contents of the invention
为克服现有技术的不足，本发明的目的是提供一种高效并且长效的适合处理焦化废水的连续生物强化工艺，有效提高了系统有效菌的数量，增加去除COD以及氨氮能力，降低后期处理焦化废水的费用及难度。In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide an efficient and long-term continuous bioaugmentation process suitable for the treatment of coking wastewater, which can effectively increase the number of effective bacteria in the system, increase the ability to remove COD and ammonia nitrogen, and reduce post-treatment The cost and difficulty of coking wastewater.
为实现上述目的，本发明通过以下技术方案实现：To achieve the above object, the present invention is achieved through the following technical solutions:
一种适合处理焦化废水的连续生物强化工艺，在好氧池活性污泥内筛分选取菌种，通过特定污染物为唯一碳源、氮源或营养基培养微生物，对选取后的菌种单独发酵培养，培养出的菌种连续不断的添加在好氧池内，包括以下步骤：A continuous bioaugmentation process suitable for the treatment of coking wastewater. The strains are screened in the activated sludge of the aerobic tank, and the microorganisms are cultivated through specific pollutants as the only carbon source, nitrogen source or nutrient medium. The selected strains are individually Fermentation culture, the cultured bacteria are continuously added in the aerobic tank, including the following steps:
1)在好氧池中取活性污泥，再将活性污泥中的菌株进行分离纯化，经过多次分离纯化以后，将所有分离纯化的菌株进行摇瓶实验，对好氧池进水进行水质分析，分析出污染物成分；1) Take the activated sludge in the aerobic tank, and then separate and purify the bacterial strains in the activated sludge. After several times of separation and purification, all the separated and purified bacterial strains are subjected to shake flask experiments, and the water quality of the influent in the aerobic tank is tested. Analysis, analysis of pollutant components;
2)以污染物作为唯一碳源、氮源或营养基，筛选出降解COD的菌株，包括若干类芽孢类菌种和若干非芽孢类菌种，还可以筛选出自养型硝化细菌与自养反硝化细菌的特效菌株；2) Use pollutants as the only carbon source, nitrogen source or nutrient base to screen out strains that degrade COD, including several spore-like bacteria and some non-spore-like bacteria, and can also screen out autotrophic nitrifying bacteria and autotrophic reaction bacteria. Specific strains of nitrifying bacteria;
3)将筛选出的降解COD的菌株进行连续流实验室小试实验，选出最佳的菌株种类，将筛选的特效菌株与降解COD的菌株加入强化车间中，对每种菌添加相对应的营养液，经过两天至七天的培养，使功能微生物的个数增加到106cfu/mL以上的数量，进入好氧池内；强化车间是处于一个不断连续的工作状态；3) Carry out the continuous flow lab test of the screened COD-degrading strains to select the best strain type, add the screened special strains and COD-degrading strains to the intensive workshop, and add corresponding The nutrient solution, after two to seven days of cultivation, increases the number of functional microorganisms to more than 106 cfu/mL and enters the aerobic pool; the intensive workshop is in a continuous working state;
4)强化车间经过一个月至三个月的连续工作过程以后，对好氧池的出水进行水质分析，与好氧池进水进行对比分析，寻找到降解程度差的污染物，以这些污染物进行唯一碳源或氮源进行菌类筛选，重复上述筛选培养菌株步骤进入强化车间发酵，加强对降解程度差的污染物的处理效果。4) After one to three months of continuous work in the intensified workshop, the water quality of the effluent from the aerobic pool was analyzed, compared with the influent water from the aerobic pool, and pollutants with poor degradation were found. The only carbon source or nitrogen source is used for fungus screening, and the above-mentioned steps of screening and cultivating strains are repeated to enter the intensive workshop for fermentation, so as to strengthen the treatment effect on pollutants with poor degradation degree.
所述的强化车间由若干个单独发酵罐组成，每个发酵罐都由控制系统、PH监测及调节管线、营养液控制管线、温度控制线路、空气加热装置、空气降温装置、曝气装置、搅拌装置、菌液输出管路组成，曝气装置、搅拌装置设置在发酵罐内，并与进气管路相连通，进气管路上连接有空气加热装置、空气降温装置，PH监测及调节管线、营养液控制管线、温度控制线路均与发酵罐内部相连通。The intensified workshop is composed of several individual fermentation tanks, and each fermentation tank is composed of a control system, pH monitoring and adjustment pipeline, nutrient solution control pipeline, temperature control circuit, air heating device, air cooling device, aeration device, stirring device, bacterial liquid output pipeline, aeration device and stirring device are set in the fermenter, and connected with the intake pipeline, the intake pipeline is connected with air heating device, air cooling device, pH monitoring and adjustment pipeline, nutrient solution Both the control pipeline and the temperature control circuit are connected with the inside of the fermenter.
所有发酵罐内温度控制在25-32℃，通过加磷酸与NaOH进行调节PH值，PH值控制在6.8-8.2，搅拌速度在120-200rpm；对于好氧型菌类，进气管路通过空气加热装置、空气降温装置后将空气消毒，通气使水中溶解氧在2-7mg/L，对于反硝化细菌，隔绝空气培养。The temperature in all fermentation tanks is controlled at 25-32°C, and the pH value is adjusted by adding phosphoric acid and NaOH. The pH value is controlled at 6.8-8.2, and the stirring speed is 120-200rpm; for aerobic bacteria, the air intake pipe is heated by air After the device and the air cooling device, the air is disinfected, and the dissolved oxygen in the water is ventilated so that the dissolved oxygen in the water is 2-7mg/L. For the denitrifying bacteria, the air is isolated for cultivation.
所述的营养液包括以下组分中的一种或两种以上：The nutrient solution includes one or more than two of the following components:
麦芽浸粉0.5-1.5g/L、酵母5-30g/L；Malt extract powder 0.5-1.5g/L, yeast 5-30g/L;
柠檬酸钠3-8mg/L、微量元素液5-30mL/L、MnSO4 0.3-1.2g/L、七水合硫酸亚铁0.05-2.8g/L、葡萄糖3-8mg/L；Sodium citrate 3-8mg/L, trace element solution 5-30mL/L, MnSO4 0.3-1.2g/L, ferrous sulfate heptahydrate 0.05-2.8g/L, glucose 3-8mg/L;
NaHCO3 0.5-5g/L、NaCl 0.1-8g/L、Na2S2O3·5H2O 1-15g/L；NaHCO3 0.5-5g/L, NaCl 0.1-8g/L, Na2 S2 O3 5H2 O 1-15g/L;
磷酸二氢钾0.2-1.5g/L、MgSO4 0.01-0.5％、磷酸氢二钾0.2-1.5g/L、KNO3 0.2-5.5g/L、CaCl2 0.2-1.5g/L。Potassium dihydrogen phosphate 0.2-1.5g/L, MgSO4 0.01-0.5%, dipotassium hydrogen phosphate 0.2-1.5g/L, KNO3 0.2-5.5g/L, CaCl2 0.2-1.5g/L.
与现有技术相比，本发明的有益效果是：Compared with prior art, the beneficial effect of the present invention is:
本工艺是在焦化废水厌氧好氧阶段中，增加活性污泥中有效菌，提高去除COD及氨氮的能力。本工艺有效提高了系统有效菌的数量，增加去除COD以及氨氮能力，改善污泥性能和产量，改善系统稳定性和可靠性，加快系统启动速度，提高了系统处理效率和减少后期处理焦化废水成本。在好氧池活性污泥内筛分选取的菌种对于好氧池内环境有更好的适应性；将每个高效菌种单独发酵培养，并添加适合其生长的营养液，能最快速度培养出需要的菌种，提高发酵效率。This process is to increase the effective bacteria in the activated sludge in the anaerobic and aerobic stage of coking wastewater, and improve the ability to remove COD and ammonia nitrogen. This process effectively increases the number of effective bacteria in the system, increases the ability to remove COD and ammonia nitrogen, improves sludge performance and output, improves system stability and reliability, speeds up system start-up, improves system treatment efficiency and reduces post-treatment coking wastewater costs. . The strains screened and selected in the activated sludge of the aerobic tank have better adaptability to the environment in the aerobic tank; each high-efficiency strain is fermented and cultivated separately, and a nutrient solution suitable for its growth is added to cultivate at the fastest speed Produce the required strains and improve the fermentation efficiency.
附图说明Description of drawings
图1是适合处理焦化废水的连续生物强化工艺的流程图。Figure 1 is a flow diagram of a continuous bioaugmentation process suitable for treating coking wastewater.
图2是强化车间工艺示意图。Figure 2 is a schematic diagram of the strengthening workshop process.
图3是出水总氰的浓度变化。Figure 3 is the concentration change of total cyanide in the effluent.
图4是出水COD的浓度变化。Figure 4 is the concentration change of effluent COD.
图中：1-控制系统 2-PH监测及调节管线 3-营养液控制管线 4-温度控制线路 5-空气加热装置 6-空气降温装置 7-曝气装置 8-搅拌装置 9-菌液输出管路。In the figure: 1-control system 2-PH monitoring and adjustment pipeline 3-nutrient solution control pipeline 4-temperature control circuit 5-air heating device 6-air cooling device 7-aeration device 8-stirring device 9-bacteria liquid output pipe road.
具体实施方式Detailed ways
下面结合说明书附图对本发明进行详细地描述，但是应该指出本发明的实施不限于以下的实施方式。The present invention will be described in detail below in conjunction with the accompanying drawings, but it should be pointed out that the implementation of the present invention is not limited to the following embodiments.
见图1，适合处理焦化废水的连续生物强化工艺，在好氧池活性污泥内筛分选取的菌种对于好氧池内环境有更好的适应性，通过特定污染物为唯一碳源、氮源或营养基培养微生物，培养出的菌种连续不断的添加在好氧池内，增强其在好氧池的活性；将每个高效菌种单独发酵培养，并添加适合其生长的营养液，能最快速度培养出需要的菌种，提高发酵效率。As shown in Figure 1, the continuous bioaugmentation process suitable for the treatment of coking wastewater, the strains screened and selected in the activated sludge in the aerobic tank have better adaptability to the environment in the aerobic tank, and the specific pollutants are the only carbon source, nitrogen source or nutrient medium to cultivate microorganisms, and the cultured strains are continuously added to the aerobic pool to enhance their activity in the aerobic pool; each high-efficiency strain is fermented and cultured separately, and a nutrient solution suitable for its growth is added, which can Cultivate the required strains at the fastest speed and improve the fermentation efficiency.
具体包括以下步骤：Specifically include the following steps:
1)在好氧池中取活性污泥，再将活性污泥中的菌株进行分离纯化，经过多次分离纯化以后，将所有分离纯化的菌株进行摇瓶实验，对好氧池进水进行水质分析，分析出污染物成分，例如常见的焦化废水中苯酚、对甲基苯酚、苯甲醇、吲哚、2,3-二甲基苯酚、异喹啉、苯胺，氰化物，苯甲醇、异喹啉、铁氰化钾。1) Take the activated sludge in the aerobic tank, and then separate and purify the bacterial strains in the activated sludge. After several times of separation and purification, all the separated and purified bacterial strains are subjected to shake flask experiments, and the water quality of the influent in the aerobic tank is tested. Analysis, analysis of pollutant components, such as phenol, p-cresol, benzyl alcohol, indole, 2,3-dimethylphenol, isoquinoline, aniline, cyanide, benzyl alcohol, isoquinol in common coking wastewater phylloline, potassium ferricyanide.
2)以污染物作为唯一碳源、氮源或营养基，筛选出高效的降解COD的菌株，包括若干类芽孢类菌种和若干非芽孢类菌种，还可以筛选出自养型硝化细菌与自养反硝化细菌的特效菌株；2) Use pollutants as the only carbon source, nitrogen source or nutrient base to screen out highly efficient COD-degrading bacterial strains, including several spore-like bacteria and some non-spore-like bacteria. Autotrophic nitrifying bacteria and autotrophic nitrifying bacteria can also be screened out. Special strains for raising denitrifying bacteria;
3)将筛选出的降解COD的菌株进行连续流实验室小试实验，选出最佳的菌株种类并确定菌株的混合比例，将筛选的特效菌株与降解COD的菌株加入强化车间中，对每种菌添加相对应的营养液，经过两天至七天的培养，使功能微生物的个数增加到106cfu/mL以上的数量，进入好氧池内；强化车间是处于一个不断连续的工作状态；3) Carry out the continuous flow laboratory test of the screened COD-degrading strains, select the best strain type and determine the mixing ratio of the strains, add the screened special strains and COD-degrading strains into the intensive workshop, and test each Add the corresponding nutrient solution to the inoculum, and after two to seven days of cultivation, the number of functional microorganisms will increase to more than 106 cfu/mL, and enter the aerobic pool; the strengthening workshop is in a continuous working state;
4)强化车间经过一个月至三个月的连续工作过程以后，对好氧池的出水进行水质分析，与好氧池进水进行对比分析，寻找到降解程度差的污染物，以这些污染物进行唯一碳源或氮源进行菌类筛选，重复上述筛选培养菌株步骤进入强化车间发酵，加强对降解程度差的污染物的处理效果。4) After one to three months of continuous work in the intensified workshop, the water quality of the effluent from the aerobic pool was analyzed, compared with the influent water from the aerobic pool, and pollutants with poor degradation were found. The only carbon source or nitrogen source is used for fungus screening, and the above-mentioned steps of screening and cultivating strains are repeated to enter the intensive workshop for fermentation, so as to strengthen the treatment effect on pollutants with poor degradation degree.
见图2，强化车间由若干个单独发酵罐组成，每个发酵罐都由控制系统1、PH监测及调节管线2、营养液控制管线3、温度控制线路4、空气加热装置5、空气降温装置6、曝气装置7、搅拌装置8、菌液输出管路9组成，曝气装置7、搅拌装置8设置在发酵罐内，并与进气管路相连通，进气管路上连接有空气加热装置5、空气降温装置6，PH监测及调节管线2、营养液控制管线3、温度控制线路4均与发酵罐内部相连通。As shown in Figure 2, the intensified workshop is composed of several individual fermentation tanks, and each fermentation tank is composed of a control system 1, a pH monitoring and adjustment pipeline 2, a nutrient solution control pipeline 3, a temperature control circuit 4, an air heating device 5, and an air cooling device 6. The aeration device 7, the stirring device 8, and the bacterial liquid output pipeline 9 are formed. The aeration device 7 and the stirring device 8 are arranged in the fermenter and communicated with the air intake pipeline, and the air heating device 5 is connected to the air intake pipeline. . The air cooling device 6, the pH monitoring and adjusting pipeline 2, the nutrient solution control pipeline 3, and the temperature control pipeline 4 are all connected to the inside of the fermenter.
所有发酵罐内温度控制在25-32℃，通过加磷酸与NaOH进行调节PH值，PH值控制在6.8-8.2，搅拌速度在120-200rpm；对于好氧型菌类，进气管路通过空气加热装置5、空气降温装置6后将空气消毒，通气使水中溶解氧在2-7mg/L，对于反硝化细菌，隔绝空气培养。The temperature in all fermentation tanks is controlled at 25-32°C, and the pH value is adjusted by adding phosphoric acid and NaOH. The pH value is controlled at 6.8-8.2, and the stirring speed is 120-200rpm; for aerobic bacteria, the air intake pipe is heated by air After the device 5 and the air cooling device 6, the air is sterilized, ventilated so that the dissolved oxygen in the water is at 2-7 mg/L, and the denitrifying bacteria are isolated from the air for cultivation.
营养液包括以下组分中的一种或两种以上：Nutrient solution includes one or more of the following components:
麦芽浸粉0.5-1.5g/L、酵母5-30g/L；Malt extract powder 0.5-1.5g/L, yeast 5-30g/L;
柠檬酸钠3-8mg/L、微量元素液5-30mL/L、MnSO4 0.3-1.2g/L、七水合硫酸亚铁0.05-2.8g/L、葡萄糖3-8mg/L；Sodium citrate 3-8mg/L, trace element solution 5-30mL/L, MnSO4 0.3-1.2g/L, ferrous sulfate heptahydrate 0.05-2.8g/L, glucose 3-8mg/L;
NaHCO3 0.5-5g/L、NaCl 0.1-8g/L、Na2S2O3·5H2O 1-15g/L；NaHCO3 0.5-5g/L, NaCl 0.1-8g/L, Na2 S2 O3 5H2 O 1-15g/L;
磷酸二氢钾0.2-1.5g/L、MgSO4 0.01-0.5％、磷酸氢二钾0.2-1.5g/L、KNO3 0.2-5.5g/L、CaCl2 0.2-1.5g/L。Potassium dihydrogen phosphate 0.2-1.5g/L, MgSO4 0.01-0.5%, dipotassium hydrogen phosphate 0.2-1.5g/L, KNO3 0.2-5.5g/L, CaCl2 0.2-1.5g/L.
实施例1：Example 1:
从活性污泥中分离纯化筛选出一种芽孢杆菌代号为DB1，以苯胺为唯一碳源，最高耐受度为800ppm，经过摇瓶实验有较高的COD去除率，去除率达到37％，将其加入强化车间，发酵罐温度控制在30℃，PH控制在7.3，搅拌速度在180rpm。通气使水中溶解氧在2-7mg/L。营养液组成：麦芽浸粉0.5g/L、MnSO40.6g/L、柠檬酸钠3mg/L、葡萄糖3mg/L、酵母5g/L、NaCl2.5g/L、七水合硫酸亚铁0.05g/L、微量元素液5mL/L、磷酸二氢钾0.2g/L、硫酸镁0.01％。培养两天，活菌数达到1.28E+08cfu/mL，细菌添加量为好氧池水量的0.05‰。A bacillus code-named DB1 was isolated and purified from activated sludge, with aniline as the only carbon source, and the highest tolerance was 800ppm. After shaking flask experiments, it had a high COD removal rate, and the removal rate reached 37%. It is added to the intensive workshop, the temperature of the fermenter is controlled at 30°C, the pH is controlled at 7.3, and the stirring speed is at 180rpm. Ventilate to keep the dissolved oxygen in the water at 2-7mg/L. Nutrient solution composition: malt extract powder 0.5g/L, MnSO4 0.6g/L, sodium citrate 3mg/L, glucose 3mg/L, yeast 5g/L, NaCl2.5g/L, ferrous sulfate heptahydrate 0.05g/L L, trace element solution 5mL/L, potassium dihydrogen phosphate 0.2g/L, magnesium sulfate 0.01%. After two days of cultivation, the number of viable bacteria reached 1.28E+08cfu/mL, and the amount of bacteria added was 0.05‰ of the water in the aerobic pool.
实施例2：Example 2:
从活性污泥中分离纯化筛选出一种芽孢杆菌代号为DB2，以总氰为唯一碳源，最高耐受度为8ppm，经过摇瓶实验有较高的COD去除率，去除率达到36％，将其加入强化车间，发酵罐温度控制在28℃，PH控制在7.5，搅拌速度在150rpm。调节通气量使水中溶解氧在2-7mg/L。营养液组成：麦芽浸粉1g/L、MnSO40.5g/L、葡萄糖3mg/L、酵母10g/L、NaCl.5g/L、七水合硫酸亚铁0.2g/L、微量元素液15mL/L、磷酸二氢钾0.8g/L、硫酸镁0.2％。培养两天，活菌数达到1.3E+06cfu/mL，细菌添加量为好氧池水量的0.01‰。A bacillus code-named DB2 was isolated and purified from activated sludge, with total cyanide as the only carbon source, and the highest tolerance was 8ppm. After shaking flask experiments, it had a high COD removal rate, and the removal rate reached 36%. It is added to the intensive workshop, the temperature of the fermenter is controlled at 28° C., the pH is controlled at 7.5, and the stirring speed is at 150 rpm. Adjust ventilation to make dissolved oxygen in water 2-7mg/L. Nutrient solution composition: malt extract powder 1g/L, MnSO4 0.5g/L, glucose 3mg/L, yeast 10g/L, NaCl.5g/L, ferrous sulfate heptahydrate 0.2g/L, trace element solution 15mL/L , potassium dihydrogen phosphate 0.8g/L, magnesium sulfate 0.2%. After two days of cultivation, the number of viable bacteria reached 1.3E+06cfu/mL, and the amount of bacteria added was 0.01‰ of the water in the aerobic pool.
实施例3Example 3
从活性污泥中分离纯化筛选出一种芽孢杆菌代号为DB3，以苯甲醇为唯一碳源，经过摇瓶实验有较高的COD去除率，去除率达到46％，将其加入强化车间，发酵罐温度控制在32℃，PH控制在7.0，搅拌速度在190rpm。调节通气量使水中溶解氧在2-7mg/L。营养液组成：麦芽浸粉1.5g/L、MnSO40.3g/L、葡萄糖5mg/L、酵母20g/L、NaCl 5.5g/L、七水合硫酸亚铁0.2g/L、微量元素液10ml/L、磷酸氢二钾0.2g/L。培养3天，活菌数达到1.36E+08cfu/mL，细菌添加量为好氧池水量的0.02‰。A bacillus code-named DB3 was isolated and purified from activated sludge, with benzyl alcohol as the only carbon source. After shaking flask experiments, it had a high COD removal rate, and the removal rate reached 46%. It was added to the intensive workshop for fermentation. The tank temperature was controlled at 32° C., the pH was controlled at 7.0, and the stirring speed was 190 rpm. Adjust ventilation to make dissolved oxygen in water 2-7mg/L. Nutrient solution composition: malt extract powder 1.5g/L, MnSO4 0.3g/L, glucose 5mg/L, yeast 20g/L, NaCl 5.5g/L, ferrous sulfate heptahydrate 0.2g/L, trace element solution 10ml/ L, dipotassium hydrogen phosphate 0.2g/L. After 3 days of cultivation, the number of viable bacteria reached 1.36E+08cfu/mL, and the amount of bacteria added was 0.02‰ of the water in the aerobic pool.
实施例4Example 4
从活性污泥中分离纯化筛选出铁基质反硝化菌代号为DB4，将其加入强化车间，发酵罐温度控制在30℃，PH控制在7.5，搅拌速度在150rpm。隔绝空气。营养液组成：FeSO4·7H2O 2.8g/L、MgSO4 0.5g/L、磷酸氢二钾0.5g/L、KH2PO4 0.5g/L、NaHCO3 3.5g/L、KNO30.5g/L、CaCl2 0.4g/L。培养7天，活菌数达到1.23E+06cfu/mL，细菌添加量为好氧池水量的0.02‰。The iron-based denitrifying bacteria code-named DB4 was isolated and purified from activated sludge, and added to the intensified workshop. The temperature of the fermenter was controlled at 30°C, the pH was controlled at 7.5, and the stirring speed was 150rpm. Insulate from air. Nutrient solution composition: FeSO4 7H2 O 2.8g/L, MgSO4 0.5g/L, dipotassium hydrogen phosphate 0.5g/L, KH2 PO4 0.5g/L, NaHCO3 3.5g/L, KNO3 0.5 g/L, CaCl2 0.4g/L. After 7 days of cultivation, the number of viable bacteria reached 1.23E+06cfu/mL, and the amount of bacteria added was 0.02‰ of the water in the aerobic pool.
实施例5Example 5
从活性污泥中分离纯化筛选出自养微生物代号为DB5，将其加入强化车间，发酵罐温度控制在30℃，PH控制在7.5，搅拌速度在150rpm。调节通气量使水中溶解氧在2-7mg/L。营养液组成：NaHCO3 2.5g/L、MgSO4 0.5g/L、KCl 0.5g/L、NH4Cl 0.5g/L。培养7天，活菌数达到2.32E+07cfu/mL，细菌添加量为好氧池水量的0.01‰。The autotrophic microorganism code-named DB5 was separated and purified from the activated sludge, and it was added to the intensified workshop. The temperature of the fermenter was controlled at 30°C, the pH was controlled at 7.5, and the stirring speed was 150rpm. Adjust ventilation to make dissolved oxygen in water 2-7mg/L. Nutrient solution composition: NaHCO3 2.5g/L, MgSO4 0.5g/L, KCl 0.5g/L, NH4 Cl 0.5g/L. After 7 days of cultivation, the number of viable bacteria reached 2.32E+07cfu/mL, and the amount of bacteria added was 0.01‰ of the water in the aerobic pool.
经过处理后的几种菌剂添加，好氧池出水的COD与氨氮都增强了净化能力，COD平均增效39％，总氰平均增效50％，见图3、图4。After the addition of several bacterial agents after treatment, the COD and ammonia nitrogen in the effluent of the aerobic pool have enhanced the purification ability, the average efficiency of COD is 39%, and the average efficiency of total cyanide is 50%, as shown in Figure 3 and Figure 4.
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| CN111825287A (en)* | 2020-08-25 | 2020-10-27 | 青岛世宇环境工程有限公司 | Tire cord production wastewater treatment system and treatment process thereof |
| CN115784515A (en)* | 2022-12-12 | 2023-03-14 | 山东太平洋环保股份有限公司 | Device and method for enhancing biological decarbonization and denitrification performance of pharmaceutical wastewater |
| CN115838630A (en)* | 2021-09-18 | 2023-03-24 | 中国石油化工股份有限公司 | Strain and preparation method thereof, microbial inoculum and influent sewage treatment method |
| CN115893696A (en)* | 2021-09-29 | 2023-04-04 | 中国石油化工股份有限公司 | Coking wastewater treatment method |
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| US20040007523A1 (en)* | 2000-10-06 | 2004-01-15 | Premier Wastewater International, Llc | Apparatus and method for wastewater treatment with enhanced solids reduction (ESR) |
| CN102041291A (en)* | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | Method for screening denitrifying bacterium |
| CN109402016A (en)* | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
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| US20040007523A1 (en)* | 2000-10-06 | 2004-01-15 | Premier Wastewater International, Llc | Apparatus and method for wastewater treatment with enhanced solids reduction (ESR) |
| CN102041291A (en)* | 2009-10-21 | 2011-05-04 | 中国石油化工股份有限公司 | Method for screening denitrifying bacterium |
| CN109402016A (en)* | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
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| CN111825287A (en)* | 2020-08-25 | 2020-10-27 | 青岛世宇环境工程有限公司 | Tire cord production wastewater treatment system and treatment process thereof |
| CN115838630A (en)* | 2021-09-18 | 2023-03-24 | 中国石油化工股份有限公司 | Strain and preparation method thereof, microbial inoculum and influent sewage treatment method |
| CN115893696A (en)* | 2021-09-29 | 2023-04-04 | 中国石油化工股份有限公司 | Coking wastewater treatment method |
| CN115784515A (en)* | 2022-12-12 | 2023-03-14 | 山东太平洋环保股份有限公司 | Device and method for enhancing biological decarbonization and denitrification performance of pharmaceutical wastewater |
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