技术领域technical field
本发明涉及核酸检测设备技术领域，尤其是涉及一种核酸检测试剂卡。The invention relates to the technical field of nucleic acid detection equipment, in particular to a nucleic acid detection reagent card.
背景技术Background technique
核酸检测在当今临床医学领域应用非常广泛，甚至在法医鉴定，先天性遗传疾病诊断、亲子鉴定等领域还作为检测的金标准。Nucleic acid detection is widely used in the field of clinical medicine today, and it is even used as the gold standard for detection in the fields of forensic identification, diagnosis of congenital genetic diseases, and paternity testing.
对于样品的核酸检测，一般分为核酸提取、核酸扩增和核酸检测三个步骤。目前商品化的核酸检测试剂产品，核酸提取、核酸扩增和核酸检测大多是独立进行的方式，前序步骤完成后再将样品移动到后序的设备中进行后序步骤的完成，相应的，前序步骤不能有效地与后序步骤集成连续进行。For nucleic acid detection of samples, it is generally divided into three steps: nucleic acid extraction, nucleic acid amplification and nucleic acid detection. At present, commercialized nucleic acid detection reagent products, nucleic acid extraction, nucleic acid amplification and nucleic acid detection are mostly carried out independently. After the previous steps are completed, the samples are moved to the subsequent equipment to complete the subsequent steps. Correspondingly, The preceding steps cannot be effectively integrated with the following steps in succession.
现有各步骤独立完成的核酸检测试剂产品，每个步骤都需要独立的设备完成，一次核酸检测过程中需要多台设备，设备占用空间大；进一步地，前序步骤完成后需要将样品移动到后序的设备中，操作繁琐，耗时较长，对环境和人员要求较高；同时，非集成的试剂产品，在前序步骤向后序步骤进行切换时，样品在移动过程中也易受到外部环境的污染或污染检测环境。Existing nucleic acid detection reagent products that complete each step independently, each step needs to be completed by independent equipment, multiple devices are required in a nucleic acid detection process, and the equipment takes up a lot of space; further, after the completion of the previous steps, the sample needs to be moved to the In the subsequent equipment, the operation is cumbersome, time-consuming, and has high requirements on the environment and personnel; at the same time, when the non-integrated reagent product is switched from the previous step to the subsequent step, the sample is also vulnerable to damage during the movement process. Contamination of the external environment or contamination detection environment.
发明内容Contents of the invention
本发明的目的在于提供一种核酸检测试剂卡，以缓解了目前商品化的核酸检测试剂产品，核酸提取、扩增和检测大多需要独立进行，不能有效地将前序步骤与后序步骤集成为一体，导致核酸检测需要多台设备，操作繁琐、耗时较长的技术问题。The purpose of the present invention is to provide a nucleic acid detection reagent card, so as to alleviate the current commercial nucleic acid detection reagent products, nucleic acid extraction, amplification and detection mostly need to be carried out independently, and it is impossible to effectively integrate the preceding steps and the following steps into one Integrate, resulting in nucleic acid detection requires multiple equipment, cumbersome operation, time-consuming technical problems.
本发明提供的一种核酸检测试剂卡，包括试剂卡本体，所述试剂卡本体上设置有样品区、提取单元、扩增单元、检测单元、动力单元以及微通道系统；A nucleic acid detection reagent card provided by the present invention includes a reagent card body, and the reagent card body is provided with a sample area, an extraction unit, an amplification unit, a detection unit, a power unit, and a microchannel system;
所述样品区设置有穿刺机构，所述样品区用于放置样品管，所述穿刺机构用于刺破样品管以将样品管内的样品引出；The sample area is provided with a puncture mechanism, the sample area is used to place a sample tube, and the puncture mechanism is used to puncture the sample tube to lead out the sample in the sample tube;
所述动力单元通过所述微通道系统分别与所述样品区内放置的样品管、所述提取单元、扩增单元和检测单元连通，且所述动力单元与所述样品区内放置的样品管之间的微通道上，所述动力单元与所述提取单元之间的微通道上、所述动力单元与所述扩增单元之间的微通道上、以及所述动力单元与所述检测单元之间的微通道上分别设置阀门，以使所述动力单元能够与所述样品区内放置的样品管、所述提取单元、所述扩增单元和所述检测单元择一连通；The power unit communicates with the sample tubes placed in the sample area, the extraction unit, the amplification unit and the detection unit respectively through the microchannel system, and the power unit communicates with the sample tubes placed in the sample area on the microchannel between the power unit and the extraction unit, on the microchannel between the power unit and the amplification unit, and between the power unit and the detection unit Valves are respectively set on the microchannels in between, so that the power unit can communicate with the sample tube placed in the sample area, the extraction unit, the amplification unit and the detection unit;
所述动力单元用于驱动液体在所述微通道系统内的流动。The power unit is used to drive the flow of liquid in the microchannel system.
进一步地，还包括洗涤容纳腔，所述洗涤容纳腔用于放置洗涤液；Further, it also includes a washing chamber, which is used to place washing liquid;
所述动力单元通过所述微通道系统与所述洗涤容纳腔连通，且所述洗涤容纳腔与所述动力单元之间的微通道上设置有阀门。The power unit communicates with the washing chamber through the microchannel system, and a valve is arranged on the microchannel between the washing chamber and the power unit.
进一步地，所述试剂卡本体上还设置有液体包腔，所述液体包腔放置有液体包，所述液体包内盛装有复溶液体；Further, the reagent card body is also provided with a liquid bag cavity, and the liquid bag cavity is placed with a liquid bag, and the liquid bag contains a reconstitution liquid;
所述动力单元通过所述微通道系统与所述液体包连通。The power unit communicates with the fluid pack through the microchannel system.
进一步地，所述动力单元包括活塞腔和活塞，所述活塞设置在所述活塞腔内，所述活塞腔与所述样品区内的样品管、所述提取单元、所述扩增单元、所述检测单元、液体包以及所述洗涤容纳腔分别通过微通道系统连通，且所述活塞腔与所述样品区内的样品管之间的微通道上、所述活塞腔与所述提取单元之间的微通道上、所述活塞腔与扩增单元之间的微通道上、所述活塞腔与检测单元之间的微通道上、所述活塞腔与所述液体包之间的微通道上、以及所述活塞腔与洗涤容纳腔之间的微通道上，分别设置有阀门。Further, the power unit includes a piston cavity and a piston, the piston is arranged in the piston cavity, the piston cavity is connected with the sample tube in the sample area, the extraction unit, the amplification unit, the The detection unit, the liquid pack, and the washing chamber are respectively connected through a microchannel system, and on the microchannel between the piston chamber and the sample tube in the sample area, between the piston chamber and the extraction unit On the microchannel between, on the microchannel between the piston chamber and the amplification unit, on the microchannel between the piston chamber and the detection unit, on the microchannel between the piston chamber and the liquid pack , and the micro-channel between the piston chamber and the washing chamber are respectively provided with valves.
进一步地，所述提取单元包括裂解液腔和吸附洗脱腔，所述裂解液腔设置有样品裂解的试剂，所述吸附洗脱腔设置有核酸捕获柱；Further, the extraction unit includes a lysate chamber and an adsorption and elution chamber, the lysate chamber is provided with reagents for sample lysis, and the adsorption and elution chamber is provided with a nucleic acid capture column;
所述裂解液腔和所述吸附洗脱腔分别与所述活塞腔通过所述微通道系统连通，且所述裂解液腔与所述活塞腔之间的微通道上、以及所述吸附洗脱腔与所述活塞腔之间的微通道上分别设置有阀门。The lysate chamber and the adsorption-elution chamber are respectively communicated with the piston chamber through the microchannel system, and on the microchannel between the lysate chamber and the piston chamber, as well as the adsorption-elution chamber The micro-channels between the cavity and the piston cavity are respectively provided with valves.
进一步地，所述扩增单元包括PCR试剂腔和PCR反应腔，PCR试剂腔和PCR反应腔分别与所述活塞腔分别通过所述微通道系统连通，且所述PCR试剂腔与所述活塞腔之间的微通道上、以及所述PCR反应腔与所述活塞腔之间的微通道上分别设置有阀门。Further, the amplification unit includes a PCR reagent chamber and a PCR reaction chamber, the PCR reagent chamber and the PCR reaction chamber communicate with the piston chamber respectively through the microchannel system, and the PCR reagent chamber and the piston chamber Valves are respectively arranged on the microchannels between the PCR reaction chamber and the piston chamber.
进一步地，所述检测单元包括杂交液腔、芯片检测腔和磁颗粒腔，所述芯片检测腔内设置有检测芯片；所述杂交液腔、所述芯片检测腔和所述磁颗粒腔分别与所述活塞腔之间通过所述微通道系统连通，且所述杂交液腔与所述活塞腔之间的微通道上、所述芯片检测腔与所述活塞腔之间的微通道上、以及所述磁颗粒腔与所述活塞腔之间的微通道上分别设置有阀门。Further, the detection unit includes a hybridization liquid chamber, a chip detection chamber and a magnetic particle chamber, and a detection chip is arranged in the chip detection chamber; the hybridization liquid chamber, the chip detection chamber and the magnetic particle chamber are respectively connected with The piston chambers are communicated through the microchannel system, and on the microchannel between the hybridization liquid chamber and the piston chamber, on the microchannel between the chip detection chamber and the piston chamber, and The micro-channels between the magnetic particle chamber and the piston chamber are respectively provided with valves.
进一步地，所述洗涤容纳腔的数量为三个，分别为第一洗涤液腔、第二洗涤液腔和第三洗涤液腔，所述第一洗涤液腔、第二洗涤液腔和第三洗涤液腔分别与所述活塞腔通过所述微通道系统连通，且所述第一洗涤液腔与所述活塞腔之间的微通道上、所述第二洗涤液腔与所述活塞腔之间的微通道上、以及所述第三洗涤液腔与所述活塞腔之间的微通道上分别设置有阀门。Further, the number of the washing chambers is three, which are respectively the first washing liquid chamber, the second washing liquid chamber and the third washing liquid chamber, and the first washing liquid chamber, the second washing liquid chamber and the third washing liquid chamber The washing liquid chamber communicates with the piston chamber respectively through the microchannel system, and on the microchannel between the first washing liquid chamber and the piston chamber, between the second washing liquid chamber and the piston chamber Valves are respectively arranged on the microchannel between the third washing liquid chamber and the piston chamber.
进一步地，所述样品区为样品管室，所述穿刺机构包括第一刺膜针管和第二刺膜针管，所述第一刺膜针管和第二刺膜针管的顶端均为尖端，且第一刺膜针管的尖端高度大于所述第二刺膜针管的尖端高度；Further, the sample area is a sample tube chamber, the puncturing mechanism includes a first lancing needle tube and a second lancing needle tube, the top ends of the first lancing needle tube and the second lancing needle tube are pointed, and the second lancing needle tube The height of the tip of the first lancet tube is greater than the height of the tip of the second puncture needle tube;
所述第一刺膜针管的下端与大气连通，所述第二刺膜针管的下端与所述提取单元通过所述微通道系统连通。The lower end of the first lancet tube communicates with the atmosphere, and the lower end of the second lancet tube communicates with the extraction unit through the microchannel system.
进一步地，所述试剂卡本体包括基座、密封底膜、卡腔密封顶膜和通道密封顶膜，所述密封底膜为弹性膜；Further, the reagent card body includes a base, a sealing bottom film, a card cavity sealing top film and a channel sealing top film, and the sealing bottom film is an elastic film;
所述基座包括一体结构的底板和功能块，所述底板的上侧面分为功能区、PCR反应区和芯片检测区，所述功能块设置在所述底板的功能区上，所述功能块上设置有活塞槽、裂解液槽、吸附洗脱槽、PCR试剂槽、杂交液槽、磁颗粒槽、洗涤槽和样品管室；The base includes a base plate and a functional block of an integrated structure, the upper side of the base plate is divided into a functional area, a PCR reaction area and a chip detection area, the functional block is arranged on the functional area of the base plate, and the functional block The top is equipped with a piston tank, a lysate tank, an adsorption and elution tank, a PCR reagent tank, a hybridization solution tank, a magnetic particle tank, a washing tank and a sample tube chamber;
所述卡腔密封顶膜密封连接在所述活塞槽、所述裂解液槽、所述吸附洗脱槽、所述PCR试剂槽、所述杂交液槽、所述磁颗粒槽以及所述洗涤槽的顶部，以形成所述活塞腔、所述裂解液腔、所述吸附洗脱腔、所述PCR试剂腔、所述杂交液腔、所述磁颗粒腔以及所述洗涤容纳腔；The sealing top membrane of the card cavity is sealed and connected to the piston tank, the lysate tank, the adsorption and elution tank, the PCR reagent tank, the hybridization solution tank, the magnetic particle tank and the washing tank to form the piston chamber, the lysate chamber, the adsorption-elution chamber, the PCR reagent chamber, the hybridization liquid chamber, the magnetic particle chamber and the washing chamber;
所述通道密封顶膜密封连接在所述底板的PCR反应区和芯片检测区的上方，所述通道密封顶膜与所述底板之间，且在所述PCR反应区和芯片检测区上分别形成所述PCR反应腔和所述芯片检测腔；The channel sealing top film is sealed and connected above the PCR reaction area and the chip detection area of the bottom plate, and the channel sealing top film and the bottom plate are respectively formed on the PCR reaction area and the chip detection area. The PCR reaction chamber and the chip detection chamber;
所述微通道系统设置在所述密封底膜与所述底板的底面之间、以及所述通道密封顶膜与所述底板之间；The microchannel system is disposed between the bottom sealing film and the bottom surface of the bottom plate, and between the channel sealing top film and the bottom plate;
所述活塞槽、所述裂解液槽、所述附洗脱槽、所述PCR试剂槽、所述杂交液槽、所述磁颗粒槽和所述洗涤槽的槽底面上分别设置有第一通孔；所述第一通孔与，所述密封底膜与所述底板的底面之间的微通道连通；所述PCR反应区和所述芯片检测区分别设置有上下连通的第二通孔，所述第二通孔将所述密封底膜与所述底板的底面之间的微通道、以及所述通道密封顶膜与所述底板之间的微通道连通。The piston tank, the lysate tank, the attached elution tank, the PCR reagent tank, the hybridization solution tank, the magnetic particle tank and the washing tank are respectively provided with a first channel on the bottom surface of the tank. hole; the first through hole communicates with the microchannel between the sealing base film and the bottom surface of the base plate; the PCR reaction area and the chip detection area are respectively provided with a second through hole communicating up and down, The second through hole communicates the microchannel between the sealing bottom film and the bottom surface of the bottom plate, and the microchannel between the channel sealing top film and the bottom plate.
进一步地，所述密封底膜与所述第一通孔之间，通过所述密封底膜与第一通孔抵接或分离形成阀门；Further, between the bottom sealing film and the first through hole, a valve is formed by the abutment or separation of the bottom sealing film and the first through hole;
所述密封底膜与所述第二通孔之间，通过所述密封底膜与所述第二通孔的抵接或分离形成阀门。Between the bottom sealing film and the second through hole, a valve is formed by abutment or separation of the bottom sealing film and the second through hole.
本发明提供的核酸检测试剂卡包括试剂卡本体，试剂卡本体上设置有样品区、提取单元、扩增单元、检测单元、动力单元以及微通道系统。其中，动力单元通过微通道系统将样品区内放置的样品管、提取单元、扩增单元和检测单元择一连通，相应的动力单元可将样品从样品管输送到提取单元，在提取单元完成相应操作后，动力单元可将样品从提取单元输送到扩增单元，样品在扩增单元完成相应操作后，动力单元又可将样品从扩增单元输送到检测单元，并在检测单元内完成最终的检测。本发明通过将样品区、提取单元、扩增单元、检测单元集成在一个试剂卡本体上，并通过动力单元和微通道系统实现各个步骤之间的样品输送，相应的核酸提取、扩增和检测三个步骤可在一个试剂卡内完成，操作简便，且不需要多台设备进行作业，相应的不需要在多台设备之间切换样品，不仅缩短了检测时间，并且有效地避免了样品易受到外部污染或者样品对环境污染的问题。The nucleic acid detection reagent card provided by the present invention includes a reagent card body, on which a sample area, an extraction unit, an amplification unit, a detection unit, a power unit and a microchannel system are arranged. Among them, the power unit connects the sample tube, extraction unit, amplification unit and detection unit placed in the sample area through the microchannel system, and the corresponding power unit can transport the sample from the sample tube to the extraction unit, and complete the corresponding process in the extraction unit. After operation, the power unit can transport the sample from the extraction unit to the amplification unit. After the sample completes the corresponding operation in the amplification unit, the power unit can transport the sample from the amplification unit to the detection unit, and complete the final detection in the detection unit. detection. The present invention integrates the sample area, extraction unit, amplification unit, and detection unit on a reagent card body, and realizes sample delivery between each step through a power unit and a microchannel system, and corresponding nucleic acid extraction, amplification, and detection The three steps can be completed in one reagent card, which is easy to operate and does not require multiple devices to operate, and correspondingly does not need to switch samples between multiple devices, which not only shortens the detection time, but also effectively avoids the sample from being susceptible to Problems with external contamination or contamination of the sample to the environment.
附图说明Description of drawings
为了更清楚地说明本发明具体实施方式或现有技术中的技术方案，下面将对具体实施方式或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图是本发明的一些实施方式，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the specific implementation of the present invention or the technical solutions in the prior art, the following will briefly introduce the accompanying drawings that need to be used in the specific implementation or description of the prior art. Obviously, the accompanying drawings in the following description The drawings show some implementations of the present invention, and those skilled in the art can obtain other drawings based on these drawings without any creative work.
图1为本发明实施例提供的核酸检测试剂卡的整体爆炸示意图；Fig. 1 is the overall explosion schematic diagram of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图2为本发明实施例提供的核酸检测试剂卡的试剂卡本体的俯视示意图；Fig. 2 is a schematic top view of the reagent card body of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图3为本发明实施例提供的核酸检测试剂卡的试剂卡本体的仰视示意图；3 is a schematic bottom view of the reagent card body of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图4为本发明实施例提供的核酸检测试剂卡的样品区的剖面图；4 is a cross-sectional view of the sample area of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图5为本发明实施例提供的核酸检测试剂卡的液体包腔的示意图；Fig. 5 is a schematic diagram of the liquid chamber of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图6为本发明实施例提供的核酸检测试剂卡的阀门的原理示意图；6 is a schematic diagram of the principle of the valve of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图7为本发明实施例提供的核酸检测试剂卡的吸附洗脱腔的示意图；7 is a schematic diagram of the adsorption and elution chamber of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图8为本发明实施例提供的核酸检测试剂卡的PCR反应腔的结构俯视图；8 is a top view of the structure of the PCR reaction chamber of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图9为图8的剖视图；Fig. 9 is a sectional view of Fig. 8;
图10为本发明实施例提供的核酸检测试剂卡的活塞腔的示意图；Fig. 10 is a schematic diagram of the piston cavity of the nucleic acid detection reagent card provided by the embodiment of the present invention;
图11为本发明实施例提供的核酸检测试剂卡的芯片检测腔的示意图。Fig. 11 is a schematic diagram of the chip detection chamber of the nucleic acid detection reagent card provided by the embodiment of the present invention.
图标：1-试剂卡本体；2-密封底膜；3-通道密封顶膜；4-液体包；5-卡腔密封顶膜；6-活塞；7-检测芯片；11-活塞腔；12-裂解液腔；13-吸附洗脱腔；14-第一洗涤液腔；15-第二洗涤液腔；16-第三洗涤液腔；17-PCR试剂腔；18-杂交液腔；19-磁颗粒腔；20-微通道系统；21-第一通孔；22-第二通孔；23-第三通孔；100-样品区；101-第一刺膜针管；102-第二刺膜针管；131-核酸捕获柱；200-液体包腔；201-穿刺针；301-样品区阀门；302-裂解液腔阀门；303-吸附洗脱腔阀门；304-第一洗涤液腔阀门；305-第二洗涤液腔阀门；306-第三洗涤液腔阀门；307-PCR试剂腔阀门；308-杂交液腔阀门；309-磁颗粒腔阀门；310-液体包腔阀门；311-第一PCR反应区阀门；312-第二PCR反应区阀门；313-芯片检测区阀门；330-压杆；400-PCR反应腔；500-芯片检测腔。Icons: 1-Reagent card body; 2-Sealing bottom membrane; 3-Channel sealing top membrane; 4-Liquid bag; 5-Card cavity sealing top membrane; 6-Piston; 7-Detection chip; Lysis solution chamber; 13-adsorption and elution chamber; 14-first washing liquid chamber; 15-second washing liquid chamber; 16-third washing liquid chamber; 17-PCR reagent chamber; 18-hybridization liquid chamber; 19-magnetic Particle cavity; 20-microchannel system; 21-first through hole; 22-second through hole; 23-third through hole; 100-sample area; ; 131-nucleic acid capture column; 200-liquid bag chamber; 201-puncture needle; 301-sample area valve; 302-lysate chamber valve; 303-adsorption and elution chamber valve; 306-the valve of the third washing liquid chamber; 307-the valve of the PCR reagent chamber; 308-the valve of the hybridization liquid chamber; 309-the valve of the magnetic particle chamber; 310-the valve of the liquid bag chamber; 311-the first PCR reaction Area valve; 312-valve in the second PCR reaction area; 313-valve in the chip detection area; 330-pressure rod; 400-PCR reaction chamber; 500-chip detection chamber.
具体实施方式Detailed ways
下面将结合实施例对本发明的技术方案进行清楚、完整地描述，显然，所描述的实施例是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
本文中涉及的PCR指的是聚合酶链式反应。PCR referred to herein refers to the polymerase chain reaction.
参考图1-图11所示，本发明实施例提供一种核酸检测试剂卡，包括试剂卡本体1，试剂卡本体1上设置有样品区100、提取单元、扩增单元、检测单元、动力单元以及微通道系统20。Referring to Figures 1-11, an embodiment of the present invention provides a nucleic acid detection reagent card, including a reagent card body 1, on which a sample area 100, an extraction unit, an amplification unit, a detection unit, and a power unit are arranged. and the microchannel system 20.
其中，样品区设置有穿刺机构，样品区100用于放置样品管，穿刺机构用于刺破样品管以将样品管内的样品引出。动力单元通过微通道系统20分别与样品区100内放置的样品管、提取单元、扩增单元和检测单元连通，且动力单元与样品区100内放置的样品管之间的微通道上，动力单元与提取单元之间的微通道上、动力单元与扩增单元之间的微通道上、以及动力单元与检测单元之间的微通道上分别设置阀门，以使动力单元能够与样品区100内放置的样品管、提取单元、扩增单元和检测单元择一连通；动力单元用于驱动液体在微通道系统20内的流动。Wherein, the sample area is provided with a puncture mechanism, the sample area 100 is used to place a sample tube, and the puncture mechanism is used to puncture the sample tube to lead out the sample in the sample tube. The power unit communicates with the sample tubes, extraction unit, amplification unit, and detection unit placed in the sample area 100 through the microchannel system 20, and on the microchannel between the power unit and the sample tubes placed in the sample area 100, the power unit Valves are set on the microchannel between the extraction unit, the microchannel between the power unit and the amplification unit, and the microchannel between the power unit and the detection unit, so that the power unit can be placed in the sample area 100 The sample tube, the extraction unit, the amplification unit and the detection unit are selectively connected; the power unit is used to drive the flow of liquid in the microchannel system 20 .
本实施例中，动力单元通过微通道系统20将样品区100内放置的样品管、提取单元、扩增单元和检测单元择一连通，相应的动力单元可将样品从样品管输送到提取单元，在提取单元完成相应操作后，动力单元可将样品从提取单元输送到扩增单元，样品在扩增单元完成相应操作后，动力单元又可将样品从扩增单元输送到检测单元，并在检测单元内完成最终的检测。本发明通过将样品区100、提取单元、扩增单元、检测单元集成在一个试剂卡本体1上，并通过动力单元和微通道系统20实现各个步骤之间样品的连通，相应的核酸提取、扩增和检测三个步骤可在一个试剂卡内完成，操作简便，且不需要多台设备进行作业，相应的不需要在多台设备之间切换样品，不仅缩短了检测时间，并且有效地避免了样品易受到外部污染或者样品对环境污染的问题。In this embodiment, the power unit communicates with one of the sample tubes, extraction unit, amplification unit, and detection unit placed in the sample area 100 through the microchannel system 20, and the corresponding power unit can transport the sample from the sample tube to the extraction unit. After the extraction unit completes the corresponding operation, the power unit can transport the sample from the extraction unit to the amplification unit, and after the sample completes the corresponding operation in the amplification unit, the power unit can transport the sample from the amplification unit to the detection unit, and the Final testing is done within the unit. The present invention integrates the sample area 100, the extraction unit, the amplification unit, and the detection unit on a reagent card body 1, and realizes the connection of samples between the various steps through the power unit and the microchannel system 20, and the corresponding nucleic acid extraction and amplification The three steps of addition and detection can be completed in one reagent card, which is easy to operate and does not require multiple devices to work, and correspondingly does not need to switch samples between multiple devices, which not only shortens the detection time, but also effectively avoids the The sample is susceptible to external contamination or the problem of sample contamination to the environment.
具体而言，提取单元包括裂解液腔12和吸附洗脱腔13，裂解液腔12设置有样品裂解的试剂，吸附洗脱腔13设置有核酸捕获柱131。裂解液腔12和吸附洗脱腔13与活塞腔11分别通过微通道系统20连通，且裂解液腔12与活塞腔11之间的微通道上、以及吸附洗脱腔13与活塞腔11之间的微通道上分别设置有阀门。其中，优选地，核酸捕获柱131采用硅基质膜材料，其可以捕获核酸分子，当洗脱液冲洗时，释放核酸分子。Specifically, the extraction unit includes a lysate chamber 12 and an adsorption and elution chamber 13 , the lysate chamber 12 is provided with reagents for lysing samples, and the adsorption and elution chamber 13 is provided with a nucleic acid capture column 131 . The lysate chamber 12 and the adsorption and elution chamber 13 communicate with the piston chamber 11 through the microchannel system 20 respectively, and on the microchannel between the lysate chamber 12 and the piston chamber 11, and between the adsorption and elution chamber 13 and the piston chamber 11 The micro-channels are respectively provided with valves. Wherein, preferably, the nucleic acid capture column 131 is made of a silicon matrix membrane material, which can capture nucleic acid molecules and release nucleic acid molecules when the eluent is washed.
扩增单元包括PCR试剂腔17和PCR反应腔400，PCR试剂腔17和PCR反应腔400与活塞腔11分别通过微通道系统20连通，且PCR试剂腔17与活塞腔11之间的微通道上、以及PCR反应腔400与活塞腔11之间的微通道上设置有阀门。Amplification unit comprises PCR reagent chamber 17 and PCR reaction chamber 400, PCR reagent chamber 17 and PCR reaction chamber 400 communicate with piston chamber 11 through microchannel system 20 respectively, and on the microchannel between PCR reagent chamber 17 and piston chamber 11 , and the microchannel between the PCR reaction chamber 400 and the piston chamber 11 is provided with a valve.
检测单元包括杂交液腔18、芯片检测腔500和磁颗粒腔19，芯片检测腔500内设置有检测芯片7；杂交液腔18、芯片检测腔500和磁颗粒腔19分别与活塞腔11之间通过微通道系统20连通，且杂交液腔18与活塞腔11之间的微通道上、芯片检测腔500与活塞腔11之间的微通道上、以及磁颗粒腔19与活塞腔11之间的微通道上分别设置有阀门。The detection unit comprises a hybridization liquid chamber 18, a chip detection chamber 500 and a magnetic particle chamber 19, and a detection chip 7 is arranged in the chip detection chamber 500; It is communicated through the microchannel system 20, and on the microchannel between the hybridization solution chamber 18 and the piston chamber 11, on the microchannel between the chip detection chamber 500 and the piston chamber 11, and between the magnetic particle chamber 19 and the piston chamber 11 The microchannels are respectively provided with valves.
作为本发明的一个可选实施例，试剂卡本体1上还包括洗涤容纳腔，洗涤容纳腔用于放置洗涤液；动力单元通过微通系统与洗涤容纳腔连通，且洗涤容纳腔与动力单元之间的微通道上设置有阀门。As an optional embodiment of the present invention, the reagent card body 1 also includes a washing chamber, which is used to place washing liquid; the power unit communicates with the washing chamber through a micro-channel system, and the connection between the washing chamber and the power unit Valves are provided on the microchannels between them.
洗涤容纳腔的数量为三个，分别为第一洗涤液腔14、第二洗涤液腔15和第三洗涤液腔16，第一洗涤液腔14、第二洗涤液腔15和第三洗涤液腔16分别与活塞腔11通过微通道系统20连通，且第一洗涤液腔14与活塞腔11之间的微通道上、第二洗涤液腔15与活塞腔11之间的微通道上、以及第三洗涤液腔16与活塞腔11之间的微通道上分别设置有阀门。The number of washing chambers is three, which are respectively the first washing liquid chamber 14, the second washing liquid chamber 15 and the third washing liquid chamber 16, the first washing liquid chamber 14, the second washing liquid chamber 15 and the third washing liquid chamber. The cavity 16 communicates with the piston cavity 11 through the microchannel system 20 respectively, and on the microchannel between the first cleaning solution cavity 14 and the piston cavity 11, on the microchannel between the second cleaning solution cavity 15 and the piston cavity 11, and The micro-channels between the third washing liquid chamber 16 and the piston chamber 11 are respectively provided with valves.
需要说明的是，洗涤容纳腔的数量不限于实施例中给出的3个的形式，可根据需要选择合适数量，按照目前的核酸检测过程中，一般需要用到3个。It should be noted that the number of washing accommodation chambers is not limited to the three forms given in the examples, and an appropriate number can be selected according to needs. According to the current nucleic acid detection process, three are generally required.
作为本发明的另一个可选实施例，试剂卡本体1上还设置有液体包腔200，液体包腔200放置有液体包4，液体包4内盛装有复溶液体；动力单元通过微通道系统20与液体包4连通。一般液体包内的复溶液体为水，液体包腔200可根据需要选择是否设置，例如，第一洗涤液腔14、第二洗涤液腔15和第三洗涤液腔16、杂交液腔18、裂解液腔12等功能腔室的试剂本身以液体的形式存在时，可不设置液体包腔200；在第一洗涤液腔14、第二洗涤液腔15和第三洗涤液腔16、杂交液腔18、裂解液腔12等功能腔室的试剂部分或全部以粉末状形式存在时，此时需要设置液体包腔200。As another optional embodiment of the present invention, the reagent card body 1 is also provided with a liquid bag cavity 200, the liquid bag cavity 200 is placed with a liquid bag 4, and the liquid bag 4 contains a reconstitution liquid; the power unit passes through the microchannel system 20 communicates with liquid bag 4. Generally, the reconstitution liquid in the liquid bag is water, and the liquid bag chamber 200 can be selected according to needs, for example, the first washing liquid chamber 14, the second washing liquid chamber 15 and the third washing liquid chamber 16, the hybridization liquid chamber 18, When the reagents in functional chambers such as the lysate chamber 12 exist in liquid form, the liquid chamber 200 may not be provided; 18. When some or all of the reagents in functional chambers such as the lysate chamber 12 exist in powder form, a liquid chamber 200 needs to be provided at this time.
如图5所示，液体包腔200上还应该设置有穿刺针201，穿刺针也是针管的形式，其可设置两个，其中一个用于刺破液体包4，通过微通道系统20与活塞腔11连接；另一个与大气连通，以维持液体包4内的气压。As shown in Figure 5, a puncture needle 201 should also be provided on the liquid bag chamber 200, and the puncture needle is also in the form of a needle tube, and it can be provided with two, one of which is used to puncture the liquid bag 4, through the microchannel system 20 and the piston chamber 11 is connected; the other communicates with the atmosphere to maintain the air pressure in the liquid bag 4.
下面结合图1-图11给出一种具体形式的核酸检测试剂卡。A specific form of nucleic acid detection reagent card is given below in conjunction with Figs. 1-11.
如图1所示，试剂卡本体1包括基座、密封底膜2、卡腔密封顶膜5和通道密封顶膜3，且密封底膜2为弹性膜。As shown in FIG. 1 , the reagent card body 1 includes a base, a sealing bottom film 2 , a card cavity sealing top film 5 and a channel sealing top film 3 , and the sealing bottom film 2 is an elastic film.
基座包括一体结构的底板和功能块，底板上的上侧面分为功能区、PCR反应区和芯片检测区，功能块设置在底板的功能区上。功能块上设置有活塞槽、裂解液槽、吸附洗脱槽、PCR试剂槽、杂交液槽、磁颗粒槽、第一洗涤槽、第二洗涤槽、第三洗涤槽、液体包槽和样品管室。卡腔密封顶膜密封连接在活塞槽、裂解液槽、吸附洗脱槽、PCR试剂槽、杂交液槽、磁颗粒槽、第一洗涤槽、第二洗涤槽、第三洗涤槽、液体包槽的顶部，以形成活塞腔11、裂解液腔12、吸附洗脱腔13、PCR试剂腔17、杂交液腔18、磁颗粒腔19、第一洗涤液腔14、第二洗涤液腔15、第三洗涤液腔16和液体包腔200。The base includes a bottom plate and a functional block of an integrated structure, the upper side of the bottom plate is divided into a functional area, a PCR reaction area and a chip detection area, and the functional block is arranged on the functional area of the bottom plate. The functional block is equipped with a piston tank, a lysate tank, an adsorption and elution tank, a PCR reagent tank, a hybridization solution tank, a magnetic particle tank, a first washing tank, a second washing tank, a third washing tank, a liquid bag tank and a sample tube room. The card cavity is sealed and the top membrane is sealed and connected to the piston tank, lysate tank, adsorption and elution tank, PCR reagent tank, hybridization solution tank, magnetic particle tank, first washing tank, second washing tank, third washing tank, liquid bag tank to form a piston chamber 11, a lysate chamber 12, an adsorption and elution chamber 13, a PCR reagent chamber 17, a hybridization liquid chamber 18, a magnetic particle chamber 19, a first washing liquid chamber 14, a second washing liquid chamber 15, and a second washing liquid chamber 15. Three washing liquid chambers 16 and liquid chambers 200 .
卡腔密封顶膜5采用双层膜结构，上层膜为铝箔，下层膜为半透膜结构，透气但不透液体。试剂卡使用前可以撕掉上层膜，下层膜粘合在试剂卡本体上，防止溶液溢出，产生污染。The sealing top film 5 of the card cavity adopts a double-layer film structure, the upper film is aluminum foil, and the lower film is a semi-permeable film structure, which is breathable but impermeable to liquid. The upper film of the reagent card can be torn off before use, and the lower film is bonded to the reagent card body to prevent the solution from overflowing and causing pollution.
通道密封顶膜3密封连接在底板的PCR反应区和芯片检测区的上方，具体可通过粘结的形式连接。通道密封顶膜3与底板之间，且在PCR反应区和芯片检测区上分别形成PCR反应腔400和芯片检测腔500。The channel-sealing top film 3 is sealed and connected above the PCR reaction area and the chip detection area of the bottom plate, specifically, it can be connected by bonding. The channel is sealed between the top membrane 3 and the bottom plate, and a PCR reaction chamber 400 and a chip detection chamber 500 are respectively formed on the PCR reaction area and the chip detection area.
密封底膜2密封连接在底板的底面上，具体可通过粘结的形式连接，微通道系统20设置在密封底膜2与底板的底面之间、以及通道密封顶膜3与底板之间；且活塞槽、裂解液槽、吸附洗脱槽、PCR试剂槽、杂交液槽、磁颗粒槽和洗涤槽的槽底面上分别设置有第一通孔21；第一通孔21与，密封底膜2与底板底面之间的微通道连通；PCR反应区和芯片检测区分别设置有上下连通的第二通孔22，第二通孔22将密封底膜2与底板的底面之间的微通道，以及通道密封顶膜3与底板之间的微通道连通。The sealing bottom film 2 is sealed and connected to the bottom surface of the bottom plate, specifically, it can be connected by bonding, and the microchannel system 20 is arranged between the bottom sealing film 2 and the bottom surface of the bottom plate, and between the channel sealing top film 3 and the bottom plate; and First through holes 21 are respectively arranged on the bottom surfaces of the piston tank, the lysate tank, the adsorption and elution tank, the PCR reagent tank, the hybridization solution tank, the magnetic particle tank and the washing tank; Communicate with the microchannel between the bottom surface of the bottom plate; the PCR reaction area and the chip detection area are respectively provided with a second through hole 22 communicating up and down, and the second through hole 22 will seal the microchannel between the bottom film 2 and the bottom surface of the bottom plate, and The microchannel between the channel sealing top membrane 3 and the bottom plate communicates.
动力单元包括活塞腔11和活塞6，活塞6设置在活塞腔11内，活塞腔11底部设置有12个第三通孔23，活塞腔11通过第三通孔23与微通道系统20连接，从而实现活塞腔11与样品区100内的样品管、提取单元、扩增单元、检测单元、液体包4以及洗涤容纳腔的分别连通。具体的，活塞腔11与样品区100内的样品管之间的微通道上设置有样品区阀门301，活塞腔11与裂解液腔12之间的微通道上设置有裂解液腔阀门302，活塞腔11与吸附洗脱腔13之间的微通道上设置有吸附洗脱腔阀门303，活塞腔11与第一洗涤液腔14之间的微通道上设置有第一洗涤液腔阀门304，活塞腔11与第二洗涤液腔15之间的微通道上设置有第二洗涤液腔阀门305，活塞腔11与第三洗涤液腔16之间的微通道上设置有第三洗涤液腔阀门306，活塞腔11与PCR试剂腔17之间的微通道上设置有PCR试剂腔阀门307，活塞腔11与杂交液腔18之间的微通道上设置有杂交液腔阀门308，活塞腔11与磁颗粒腔19之间的微通道上设置有磁颗粒腔阀门309，活塞腔11与液体包腔200之间的微通道上设置有液体包腔阀门310，活塞腔11与PCR反应腔400室之间的微通道上设置有第一PCR反应区阀门311和第二PCR反应区阀门312，活塞腔11与芯片检测腔500之间的微通道上设置有芯片检测区阀门313。Power unit comprises piston chamber 11 and piston 6, and piston 6 is arranged in piston chamber 11, and the bottom of piston chamber 11 is provided with 12 third through holes 23, and piston chamber 11 is connected with microchannel system 20 by the 3rd through hole 23, thereby The communication between the piston chamber 11 and the sample tube, extraction unit, amplification unit, detection unit, liquid bag 4 and washing chamber in the sample area 100 is achieved. Specifically, a sample area valve 301 is provided on the microchannel between the piston chamber 11 and the sample tube in the sample area 100, and a lysate chamber valve 302 is provided on the microchannel between the piston chamber 11 and the lysate chamber 12. The micro-channel between the chamber 11 and the adsorption-elution chamber 13 is provided with an adsorption-elution chamber valve 303, and the micro-channel between the piston chamber 11 and the first washing liquid chamber 14 is provided with a first washing liquid chamber valve 304, and the piston A second washing liquid chamber valve 305 is provided on the microchannel between the chamber 11 and the second washing liquid chamber 15, and a third washing liquid chamber valve 306 is provided on the microchannel between the piston chamber 11 and the third washing liquid chamber 16. , the microchannel between the piston chamber 11 and the PCR reagent chamber 17 is provided with a PCR reagent chamber valve 307, and the microchannel between the piston chamber 11 and the hybridization liquid chamber 18 is provided with a hybridization liquid chamber valve 308, and the piston chamber 11 and the magnetic A magnetic particle chamber valve 309 is provided on the microchannel between the particle chambers 19, a liquid chamber valve 310 is provided on the microchannel between the piston chamber 11 and the liquid chamber 200, and a valve 310 is provided between the piston chamber 11 and the PCR reaction chamber 400. The first PCR reaction area valve 311 and the second PCR reaction area valve 312 are arranged on the microchannel, and the chip detection area valve 313 is arranged on the microchannel between the piston chamber 11 and the chip detection chamber 500 .
具体的，样品区阀门301、裂解液腔阀门302、吸附洗脱腔阀门303、第一洗涤液腔阀门304、第二洗涤液腔阀门305、第三洗涤液腔阀门306、PCR试剂腔阀门307、杂交液腔阀门308、磁颗粒腔阀门309和液体包腔阀门310均设置在密封底膜2与各个腔室底部的第一通孔21之间，并通过密封底膜2与第一通孔21抵接或分离形成阀门。具体的原理如图6所示。Specifically, the sample area valve 301, the lysate chamber valve 302, the adsorption and elution chamber valve 303, the first washing liquid chamber valve 304, the second washing liquid chamber valve 305, the third washing liquid chamber valve 306, and the PCR reagent chamber valve 307 , the hybrid liquid cavity valve 308, the magnetic particle cavity valve 309 and the liquid-encapsulated cavity valve 310 are all arranged between the sealing bottom film 2 and the first through hole 21 at the bottom of each chamber, and through the sealing bottom film 2 and the first through hole 21 abutting or separating to form a valve. The specific principle is shown in Figure 6.
第一PCR反应区阀门311、第二PCR反应区阀门312和芯片检测区阀门313均设置在密封底膜2与第二通孔22之间，且通过密封底膜2与第二通孔22的抵接或分离形成阀门。具体的原理也可参照图6所示。The first PCR reaction area valve 311, the second PCR reaction area valve 312 and the chip detection area valve 313 are all arranged between the sealing bottom film 2 and the second through hole 22, and through the sealing bottom film 2 and the second through hole 22 abut or separate to form a valve. The specific principle can also be referred to as shown in FIG. 6 .
样品区阀门301用于控制样本的注入，裂解液腔阀门302、吸附洗脱腔阀门303、第一洗涤液腔阀门304、第二洗涤液腔阀门305、第三洗涤液腔阀门306、PCR试剂腔阀门307、杂交液腔阀门308、磁颗粒腔阀门309和液体包腔阀门310分别控制其各自对应的腔室与活塞腔11的连通。The valve 301 in the sample area is used to control the injection of the sample, the valve 302 of the lysate chamber, the valve 303 of the adsorption and elution chamber, the valve 304 of the first washing liquid chamber, the valve 305 of the second washing liquid chamber, the valve 306 of the third washing liquid chamber, and the PCR reagent The chamber valve 307 , the hybridization liquid chamber valve 308 , the magnetic particle chamber valve 309 and the liquid chamber valve 310 respectively control the communication between their corresponding chambers and the piston chamber 11 .
样品区100为样品管室，穿刺机构包括第一刺膜针管101和第二刺膜针管102，第一刺膜针管101和第二刺膜针管102的顶端均为尖端，且第一刺膜针管101的尖端高度大于第二刺膜针管102的尖端高度；第一刺膜针管101的下端与大气连通，第二刺膜针管102的下端与提取单元通过微通道系统20连通。The sample area 100 is a sample tube chamber, and the puncture mechanism includes a first lancet tube 101 and a second lancet tube 102, the tops of the first puncture needle tube 101 and the second puncture needle tube 102 are pointed, and the first puncture needle tube The tip height of 101 is greater than that of the second puncture needle tube 102; the lower end of the first puncture needle tube 101 communicates with the atmosphere, and the lower end of the second puncture needle tube 102 communicates with the extraction unit through the microchannel system 20.
当样品管插入到样品区100后，第一刺膜针管101通过微通道系统20和通孔与大气相通，用于平衡样品管的内部气压。第二刺膜针管102通过微通道系统20和样品区阀门301与活塞腔11连通，用于吸取样品。第一刺膜针管101和第二刺膜针管102的针头切面相对，并且通过第一刺膜针管101高于第二刺膜针管102，以防止气泡被吸入活塞腔11。活塞6组装在活塞腔11中，可以通过外部机械结构带动活塞6在活塞腔11上下运动，从而实现抽取液体和挤压液体的作用。并且，通过与各个阀门的协同工作，可以选择的移动液体到各个腔体和功能区。After the sample tube is inserted into the sample area 100, the first lancing needle tube 101 communicates with the atmosphere through the microchannel system 20 and the through hole, which is used to balance the internal air pressure of the sample tube. The second piercing needle tube 102 communicates with the piston chamber 11 through the microchannel system 20 and the sample area valve 301 for sucking the sample. The first lancet tube 101 and the second puncture needle tube 102 have opposite needle sections, and the first puncture needle tube 101 is higher than the second lancet tube 102 to prevent air bubbles from being sucked into the piston cavity 11 . The piston 6 is assembled in the piston chamber 11, and the external mechanical structure can drive the piston 6 to move up and down in the piston chamber 11, so as to realize the functions of extracting liquid and squeezing liquid. And, by cooperating with various valves, liquid can be selectively moved to various cavities and functional areas.
PCR反应腔400由试剂卡本体1和通道密封顶膜3构成，前后有第一PCR反应区阀门311和第二PCR反应区阀门，当PCR反应过程中，第一PCR反应区阀门311和第二PCR反应区阀门312关闭，形成密封腔体；有效地避免了气溶胶的污染。The PCR reaction chamber 400 is composed of a reagent card body 1 and a channel sealing top membrane 3. There are a first PCR reaction area valve 311 and a second PCR reaction area valve before and after it. During the PCR reaction process, the first PCR reaction area valve 311 and the second PCR reaction area valve The valve 312 in the PCR reaction area is closed to form a sealed chamber; effectively avoiding aerosol pollution.
本实施例通过阀门的挤压和放松来控制活塞腔11与试剂腔、功能区各腔室的相互连通，然后利用活塞6的上下运动提供液体在各个腔室和功能区之间转移所需的动力，从而完成裂解、洗涤、吸附、洗脱、PCR扩增、核酸杂交检测等操作。具体的，当采用压杆330挤压阀门区域的通道密封底膜2时，密封底膜2与对应的腔室底部的通孔抵接，使得腔室底部的通孔的底部堵塞，也即阀门关闭，活塞腔11与试剂腔和功能区断开。当放松压阀门区域的通道密封底膜2时，密封底膜2与对应的腔室底部的通孔远离，阀门打开，活塞腔11与试剂腔和功能区连通。需要说明的是，在一次操作过程中，活塞腔11仅仅可与其中一个腔室连通，例如，在进样品时，除了样品区阀门301打开之外，其他阀门均处于关闭状态，此时抽拉活塞6，可将样品从样品管内抽取至活塞腔11内；而后，将样品区阀门301关闭，相应的打开裂解液腔阀门302，通过推动活塞6，将活塞腔11内的样品注入到裂解液腔12。其他过程的样品转移或液体转移，与进样品时相似，在此不再赘述。In this embodiment, the mutual communication between the piston chamber 11 and the reagent chamber and each chamber of the functional area is controlled by the extrusion and relaxation of the valve, and then the up and down movement of the piston 6 is used to provide the liquid needed for the transfer between each chamber and the functional area. Power, so as to complete lysis, washing, adsorption, elution, PCR amplification, nucleic acid hybridization detection and other operations. Specifically, when the pressure rod 330 is used to squeeze the channel sealing bottom film 2 in the valve area, the sealing bottom film 2 abuts against the through hole at the bottom of the corresponding chamber, so that the bottom of the through hole at the bottom of the chamber is blocked, that is, the valve Closed, the piston chamber 11 is disconnected from the reagent chamber and the functional area. When the channel sealing base film 2 in the pressure valve area is relaxed, the sealing base film 2 is away from the through hole at the bottom of the corresponding chamber, the valve is opened, and the piston chamber 11 communicates with the reagent chamber and the functional area. It should be noted that during one operation, the piston chamber 11 can only communicate with one of the chambers. For example, when the sample is injected, except for the sample area valve 301, the other valves are closed. The piston 6 can extract the sample from the sample tube into the piston chamber 11; then, close the valve 301 in the sample area, open the valve 302 of the lysate chamber correspondingly, and inject the sample in the piston chamber 11 into the lysate by pushing the piston 6 Cavity 12. The sample transfer or liquid transfer in other processes is similar to that of sample feeding, and will not be repeated here.
本实施例中，裂解液腔12内的试剂可以是异硫氰酸胍、三(羟甲基)氨基甲烷(Tris-HCl)、乙二胺四乙酸(EDTA)、聚乙二醇辛基苯基醚(Trition-X100)、十二烷基硫酸钠(SDS)的水溶液，第一洗涤液可以为异硫氰酸胍、三(羟甲基)氨基甲烷(Tris)水溶液，第二洗涤液可为两种，一种是不进行冻干的配方：乙醇、氯化钠，三(羟甲基)氨基甲烷(Tris-HCl)的溶液，另一种是可以冻干的配方：氯化钠、聚乙二醇(PEG)8000、三(羟甲基)氨基甲烷(Tris-HCl)的溶液；第三洗涤液腔16内的液体为碳酸氢钠、碳酸钠、吐温-20的缓冲液，PCR试剂腔17的试剂：扩增引物、MMLV逆转录酶、Taq酶(Taq酶是从水生栖热菌ThermusAquaticus中分离出的具有热稳定性的DNA聚合酶)、dNTPs(脱氧核糖核苷酸)、硫酸铵、氯化镁等Tris(Tris中文品名为三羟甲基氨基甲烷)缓冲液。杂交液腔18的试剂可为两种，一种不进行冻干的配方：甲酰胺、十二烷基硫酸钠(SDS)，柠檬酸钠缓冲液，可以冻干的配方：碳酸乙烯酯、十二烷基硫酸钠(SDS)，柠檬酸钠缓冲液。磁颗粒腔19的溶液为磁珠、牛血清白蛋白(BSA)、碳酸盐缓冲液。In this embodiment, the reagent in the lysate chamber 12 can be guanidine isothiocyanate, tris(hydroxymethyl)aminomethane (Tris-HCl), ethylenediaminetetraacetic acid (EDTA), polyethylene glycol octylbenzene base ether (Trition-X100), sodium dodecyl sulfate (SDS) aqueous solution, the first washing liquid can be guanidine isothiocyanate, tris (hydroxymethyl) aminomethane (Tris) aqueous solution, the second washing liquid can be There are two kinds, one is the formula without lyophilization: ethanol, sodium chloride, tris (hydroxymethyl) aminomethane (Tris-HCl) solution, and the other is the formula that can be lyophilized: sodium chloride, The solution of Polyethylene Glycol (PEG) 8000, tris (hydroxymethyl) aminomethane (Tris-HCl); The liquid in the 3rd cleaning solution chamber 16 is the buffer solution of sodium bicarbonate, sodium carbonate, Tween-20, Reagents in PCR reagent chamber 17: amplification primers, MMLV reverse transcriptase, Taq enzyme (Taq enzyme is a thermostable DNA polymerase isolated from Thermus Aquaticus), dNTPs (deoxyribonucleotides) , ammonium sulfate, magnesium chloride and other Tris (Tris Chinese product name is trishydroxymethylaminomethane) buffer solution. The reagent of the hybridization liquid cavity 18 can be two kinds, a kind of formula that does not carry out lyophilization: formamide, sodium dodecyl sulfate (SDS), sodium citrate buffer solution, the formula that can be lyophilized: ethylene carbonate, ten Sodium Dialkyl Sulfate (SDS), Sodium Citrate Buffer. The solution in the magnetic particle cavity 19 is magnetic beads, bovine serum albumin (BSA), and carbonate buffer.
检测芯片7可以通过检测磁敏杂交方式对核酸进行检测，但不限于磁敏杂交方式对核酸进行检测。优选地，检测芯片7采用巨磁阻芯片，表面固定杂交探针，可以与扩增后带有生物素基团的目标核酸进行杂交，使之结合在芯片表面，之后链霉亲和素表面的磁性纳米颗粒通过生物素-亲和素反应结合在芯片表面，巨磁阻芯片通过检测固定在芯片表面的磁性纳米颗粒产生的磁信号对核酸进行检测。The detection chip 7 can detect nucleic acid by detecting magnetic sensitive hybridization, but is not limited to detecting nucleic acid by magnetic sensitive hybridization. Preferably, the detection chip 7 adopts a giant magnetoresistive chip, and the hybridization probe is immobilized on the surface, which can be hybridized with the amplified target nucleic acid with a biotin group, so that it is bound to the chip surface, and then the streptavidin surface Magnetic nanoparticles are bound to the surface of the chip through a biotin-avidin reaction, and the giant magnetoresistive chip detects nucleic acids by detecting the magnetic signals generated by the magnetic nanoparticles fixed on the surface of the chip.
本实施例中优选的，核酸提取、扩增和检测试剂采用冻干粉形式封装在试剂卡中。Preferably in this embodiment, nucleic acid extraction, amplification and detection reagents are packaged in reagent cards in the form of lyophilized powder.
通过阀门的挤压和放松来控制活塞腔11与试剂腔和功能区的相互连通，然后利用活塞6的上下运动转移液体到各个腔室和功能区。下面给出本实施例核酸检测试剂卡的一个具体的应用过程：一个整体使用过程的：The mutual communication between the piston chamber 11 and the reagent chamber and the functional area is controlled by the squeeze and release of the valve, and then the liquid is transferred to each chamber and functional area by the up and down movement of the piston 6 . A specific application process of the nucleic acid detection reagent card of this embodiment is given below: an overall use process:
步骤一、复溶试剂过程Step 1. Process of redissolving reagents
采用活塞6分别吸取液体包4内溶液50ul，20ul，300ul，300ul，300ul，分别注入到杂交液腔18，磁颗粒腔19，第一洗涤液腔，第二洗涤液腔，第三洗涤液腔；活塞每次进行一次液体的吸取，每次进行一次液体的注入，且在活塞执行液体注入的过程中，可上下移动活塞6对其中的试剂进行快速复溶。Use the piston 6 to draw 50ul, 20ul, 300ul, 300ul, and 300ul of the solution in the liquid bag 4, respectively, and inject them into the hybridization liquid chamber 18, the magnetic particle chamber 19, the first washing liquid chamber, the second washing liquid chamber, and the third washing liquid chamber The piston absorbs the liquid once each time, and injects the liquid once each time, and during the liquid injection process of the piston, the piston 6 can be moved up and down to quickly redissolve the reagent therein.
步骤二、核酸提取过程Step 2. Nucleic acid extraction process
样本管插入样本管连接区，采用活塞6吸取200ul样本注入到裂解液腔，上下移动活塞6方式复溶裂解液，该过程对样本进行裂解。裂解过程还可以在裂解液腔底部加入超声处理，提高裂解效率。The sample tube is inserted into the connection area of the sample tube, the piston 6 is used to draw 200ul of the sample and injected into the lysate chamber, and the lysate is redissolved by moving the piston 6 up and down, and the sample is lysed in this process. During the lysis process, ultrasonic treatment can also be added at the bottom of the lysate chamber to improve the lysis efficiency.
裂解后，采用活塞6把裂解混合液注入到吸附洗脱腔，使核酸吸附到吸附柱上，废液注回裂解液腔。然后通过活塞分别吸取第一清洗液清洗和第二清洗液对吸附柱进行反复清洗，废液注回裂解液腔。最后从液体包4中吸取溶液50ul注入到吸附洗脱腔，对核酸进行洗脱。After the lysis, the piston 6 is used to inject the lysis mixture into the adsorption and elution chamber, so that the nucleic acid is adsorbed on the adsorption column, and the waste liquid is injected back into the lysis solution chamber. Then, the piston absorbs the first cleaning solution and the second cleaning solution to clean the adsorption column repeatedly, and the waste liquid is injected back into the lysate chamber. Finally, 50 ul of the solution is sucked from the liquid bag 4 and injected into the adsorption and elution chamber to elute the nucleic acid.
步骤三、PCR扩增过程：Step 3, PCR amplification process:
采用活塞6吸取洗脱液注入到PCR试剂腔17复溶PCR试剂，上下移动活塞6，使之复溶均匀。然后把溶解后的PCR试剂混合液注入到PCR反应区。经过30次升温降温循环，完成核酸的扩增过程。Use the piston 6 to absorb the eluent and inject it into the PCR reagent chamber 17 to redissolve the PCR reagent, and move the piston 6 up and down to make the redissolution uniform. Then inject the dissolved PCR reagent mixture into the PCR reaction area. After 30 heating and cooling cycles, the nucleic acid amplification process is completed.
步骤四、杂交检测过程Step 4. Hybridization detection process
采用活塞6把扩增后的PCR溶液吸入活塞腔11，然后吸取杂交液100ul，与之混合均匀后注入到芯片检测区。芯片表面固定杂交探针，可以与扩增后带有生物素基团的目标核酸进行杂交，使之结合在芯片表面。之后，活塞6吸取第三清洗液清洗芯片表面，然后注入链霉亲和素表面的磁性纳米颗粒到芯片检测区，通过生物素-亲和素反应结合在芯片表面，巨磁阻芯片通过检测固定在芯片表面的磁性纳米颗粒产生的磁信号对核酸进行检测。Use the plunger 6 to suck the amplified PCR solution into the plunger cavity 11, then draw 100ul of the hybridization solution, mix it evenly, and inject it into the detection area of the chip. Immobilized hybridization probes on the surface of the chip can hybridize with the amplified target nucleic acid with a biotin group to bind to the surface of the chip. After that, the piston 6 absorbs the third cleaning solution to clean the surface of the chip, and then injects the magnetic nanoparticles on the surface of the streptavidin into the detection area of the chip, and binds to the surface of the chip through a biotin-avidin reaction, and the giant magnetoresistive chip is fixed by detection. Nucleic acids are detected by magnetic signals generated by magnetic nanoparticles on the surface of the chip.
本发明实施例检测芯片封装在微通道系统当中，可以通过磁敏杂交方式对核酸进行检测。本发明实现核酸提取、扩增和检测集成在一个试剂卡中，不仅简化了操作流程，缩短了时间，减少了反应体积，实现自动化检测目的，并且有效地避免了外部污染以及气溶胶的污染。同时，采用微流控技术和磁敏杂交技术降低了样本用量，提高了核酸提取和扩增的效率，提升了检测灵敏度和特异性。The detection chip of the embodiment of the present invention is packaged in the microchannel system, and can detect nucleic acid by magnetic sensitive hybridization. The invention integrates nucleic acid extraction, amplification and detection in one reagent card, which not only simplifies the operation process, shortens the time, reduces the reaction volume, realizes the purpose of automatic detection, and effectively avoids external pollution and aerosol pollution. At the same time, the use of microfluidic technology and magnetic sensitive hybridization technology reduces the amount of samples, improves the efficiency of nucleic acid extraction and amplification, and improves the detection sensitivity and specificity.
最后应说明的是：以上各实施例仅用以说明本发明的技术方案，而非对其限制；尽管参照前述各实施例对本发明进行了详细的说明，本领域的普通技术人员应当理解：其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分或者全部技术特征进行等同替换；而这些修改或者替换，并不使相应技术方案的本质脱离本发明各实施例技术方案的范围。Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910647829.3ACN110257245A (en) | 2019-07-16 | 2019-07-16 | Nucleic acid detection reagent card |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910647829.3ACN110257245A (en) | 2019-07-16 | 2019-07-16 | Nucleic acid detection reagent card |
| Publication Number | Publication Date |
|---|---|
| CN110257245Atrue CN110257245A (en) | 2019-09-20 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910647829.3APendingCN110257245A (en) | 2019-07-16 | 2019-07-16 | Nucleic acid detection reagent card |
| Country | Link |
|---|---|
| CN (1) | CN110257245A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110964715A (en)* | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
| CN110964716A (en)* | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
| CN111019937A (en)* | 2019-12-05 | 2020-04-17 | 广州万孚生物技术股份有限公司 | Extraction method applied to in-vitro diagnosis and analysis device |
| CN111394243A (en)* | 2020-04-27 | 2020-07-10 | 东莞博识生物科技有限公司 | Micro-fluidic chip nucleic acid detection device |
| CN111454834A (en)* | 2020-04-08 | 2020-07-28 | 中国科学院长春光学精密机械与物理研究所 | PCR Amplifier |
| CN111704993A (en)* | 2020-08-18 | 2020-09-25 | 中国农业大学 | An integrated nucleic acid POCT detection device and method |
| CN112126589A (en)* | 2020-10-13 | 2020-12-25 | 南京中科拜尔医学技术有限公司 | Autonomous liquid suction type nucleic acid detection card box based on magnetic rod method and use method |
| CN112779248A (en)* | 2021-02-04 | 2021-05-11 | 杭州遂曾生物技术有限公司 | Chlamydia trachomatis integrated nucleic acid detection card box |
| CN112812959A (en)* | 2019-11-15 | 2021-05-18 | 广州万孚生物技术股份有限公司 | Nucleic acid extraction, amplification and detection integrated device |
| CN113278609A (en)* | 2021-06-03 | 2021-08-20 | 广西产研院生物制造技术研究所有限公司 | Regeneration method of silicon substrate nucleic acid purification column |
| CN113667591A (en)* | 2020-05-15 | 2021-11-19 | 北京百康芯生物科技有限公司 | Full-automatic nucleic acid detection and analysis system |
| WO2022061521A1 (en)* | 2020-09-22 | 2022-03-31 | 京东方科技集团股份有限公司 | Microfluidic chip for nucleic acid extraction, and nucleic acid extraction device and method |
| CN114487455A (en)* | 2022-02-14 | 2022-05-13 | 广州万孚生物技术股份有限公司 | In vitro diagnostic instrument and its control method |
| CN114686353A (en)* | 2020-12-31 | 2022-07-01 | 中元汇吉生物技术股份有限公司 | Detection module and detection device |
| CN114686365A (en)* | 2020-12-31 | 2022-07-01 | 中元汇吉生物技术股份有限公司 | Reagent card |
| WO2022242499A1 (en)* | 2021-05-18 | 2022-11-24 | 成都万众壹芯生物科技有限公司 | Microfluidic nucleic acid detection kit and detection device |
| WO2023274301A1 (en)* | 2021-06-30 | 2023-01-05 | 恒泰医疗有限公司 | Chip device and instrument for nucleic acid detection, and application thereof |
| CN118048223A (en)* | 2024-03-29 | 2024-05-17 | 中国科学院过程工程研究所 | Nucleic acid extraction device |
| CN118185730A (en)* | 2024-03-29 | 2024-06-14 | 中国科学院过程工程研究所 | A nucleic acid extraction system |
| EP4495218A4 (en)* | 2022-04-15 | 2025-08-06 | Guangdong Runpeng Biological Tech Co Ltd | REAGENT CARTRIDGE, DETECTION DEVICE AND DETECTION METHOD |
| US12417961B2 (en) | 2022-01-28 | 2025-09-16 | Beijing Boe Technology Development Co., Ltd. | Chip heat sink and nucleic acid extraction device |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1973197A (en)* | 2004-01-28 | 2007-05-30 | 挪其普公司 | A diagnostic system for carrying out a nucleic acid sequence amplification and detection process |
| WO2007106580A2 (en)* | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Rapid magnetic flow assays |
| CN101541962A (en)* | 2007-03-23 | 2009-09-23 | 株式会社东芝 | Nucleic acid detection cassette and nucleic acid detection apparatus |
| CN104673625A (en)* | 2015-02-13 | 2015-06-03 | 西安交通大学 | Automatic reaction device and method for pretreating cells |
| CN105349401A (en)* | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof |
| CN106460233A (en)* | 2014-04-14 | 2017-02-22 | 思研（Sri）国际顾问与咨询公司 | Portable nucleic acid analysis systemand high-performance microfluidic electroactive polymer actuators |
| KR20170024827A (en)* | 2015-08-26 | 2017-03-08 | (주)바이오니아 | The Quantitative PCR Cartridge with Microchannel-Film Reactor, Nucleic Acid Extraction Module and qPCR Reagents Module, and The Rapid qPCR System Using the Same |
| CN107723210A (en)* | 2017-11-19 | 2018-02-23 | 杭州安弼晟生物科技有限公司 | Novel nucleic acids detect micro flow control chip device |
| CN107893020A (en)* | 2017-11-27 | 2018-04-10 | 深圳华炎微测医疗科技有限公司 | Molecule diagnoses micro-fluidic chip and molecule diagnosis micro-fluidic chip system and their application |
| CN210506362U (en)* | 2019-07-16 | 2020-05-12 | 东莞博识生物科技有限公司 | Nucleic acid detection reagent card |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1973197A (en)* | 2004-01-28 | 2007-05-30 | 挪其普公司 | A diagnostic system for carrying out a nucleic acid sequence amplification and detection process |
| WO2007106580A2 (en)* | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Rapid magnetic flow assays |
| CN101541962A (en)* | 2007-03-23 | 2009-09-23 | 株式会社东芝 | Nucleic acid detection cassette and nucleic acid detection apparatus |
| CN106460233A (en)* | 2014-04-14 | 2017-02-22 | 思研（Sri）国际顾问与咨询公司 | Portable nucleic acid analysis systemand high-performance microfluidic electroactive polymer actuators |
| CN104673625A (en)* | 2015-02-13 | 2015-06-03 | 西安交通大学 | Automatic reaction device and method for pretreating cells |
| KR20170024827A (en)* | 2015-08-26 | 2017-03-08 | (주)바이오니아 | The Quantitative PCR Cartridge with Microchannel-Film Reactor, Nucleic Acid Extraction Module and qPCR Reagents Module, and The Rapid qPCR System Using the Same |
| CN105349401A (en)* | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof |
| CN107723210A (en)* | 2017-11-19 | 2018-02-23 | 杭州安弼晟生物科技有限公司 | Novel nucleic acids detect micro flow control chip device |
| CN107893020A (en)* | 2017-11-27 | 2018-04-10 | 深圳华炎微测医疗科技有限公司 | Molecule diagnoses micro-fluidic chip and molecule diagnosis micro-fluidic chip system and their application |
| CN210506362U (en)* | 2019-07-16 | 2020-05-12 | 东莞博识生物科技有限公司 | Nucleic acid detection reagent card |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812959A (en)* | 2019-11-15 | 2021-05-18 | 广州万孚生物技术股份有限公司 | Nucleic acid extraction, amplification and detection integrated device |
| CN110964716A (en)* | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
| CN111019937A (en)* | 2019-12-05 | 2020-04-17 | 广州万孚生物技术股份有限公司 | Extraction method applied to in-vitro diagnosis and analysis device |
| CN111019937B (en)* | 2019-12-05 | 2022-03-08 | 广州万孚生物技术股份有限公司 | Extraction method applied to in-vitro diagnosis and analysis device |
| CN110964715A (en)* | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
| CN111454834A (en)* | 2020-04-08 | 2020-07-28 | 中国科学院长春光学精密机械与物理研究所 | PCR Amplifier |
| CN111454834B (en)* | 2020-04-08 | 2021-12-03 | 中国科学院长春光学精密机械与物理研究所 | PCR augmentor |
| CN111394243A (en)* | 2020-04-27 | 2020-07-10 | 东莞博识生物科技有限公司 | Micro-fluidic chip nucleic acid detection device |
| CN113667591A (en)* | 2020-05-15 | 2021-11-19 | 北京百康芯生物科技有限公司 | Full-automatic nucleic acid detection and analysis system |
| CN111704993B (en)* | 2020-08-18 | 2020-11-20 | 中国农业大学 | An integrated nucleic acid POCT detection device and method |
| CN111704993A (en)* | 2020-08-18 | 2020-09-25 | 中国农业大学 | An integrated nucleic acid POCT detection device and method |
| WO2022061521A1 (en)* | 2020-09-22 | 2022-03-31 | 京东方科技集团股份有限公司 | Microfluidic chip for nucleic acid extraction, and nucleic acid extraction device and method |
| CN112126589A (en)* | 2020-10-13 | 2020-12-25 | 南京中科拜尔医学技术有限公司 | Autonomous liquid suction type nucleic acid detection card box based on magnetic rod method and use method |
| CN114686353A (en)* | 2020-12-31 | 2022-07-01 | 中元汇吉生物技术股份有限公司 | Detection module and detection device |
| CN114686365A (en)* | 2020-12-31 | 2022-07-01 | 中元汇吉生物技术股份有限公司 | Reagent card |
| CN112779248A (en)* | 2021-02-04 | 2021-05-11 | 杭州遂曾生物技术有限公司 | Chlamydia trachomatis integrated nucleic acid detection card box |
| WO2022242499A1 (en)* | 2021-05-18 | 2022-11-24 | 成都万众壹芯生物科技有限公司 | Microfluidic nucleic acid detection kit and detection device |
| CN113278609A (en)* | 2021-06-03 | 2021-08-20 | 广西产研院生物制造技术研究所有限公司 | Regeneration method of silicon substrate nucleic acid purification column |
| CN113278609B (en)* | 2021-06-03 | 2024-02-13 | 广西产研院生物制造技术研究所有限公司 | Regeneration method of silicon substrate nucleic acid purification column |
| WO2023274301A1 (en)* | 2021-06-30 | 2023-01-05 | 恒泰医疗有限公司 | Chip device and instrument for nucleic acid detection, and application thereof |
| US12417961B2 (en) | 2022-01-28 | 2025-09-16 | Beijing Boe Technology Development Co., Ltd. | Chip heat sink and nucleic acid extraction device |
| CN114487455A (en)* | 2022-02-14 | 2022-05-13 | 广州万孚生物技术股份有限公司 | In vitro diagnostic instrument and its control method |
| EP4495218A4 (en)* | 2022-04-15 | 2025-08-06 | Guangdong Runpeng Biological Tech Co Ltd | REAGENT CARTRIDGE, DETECTION DEVICE AND DETECTION METHOD |
| CN118048223A (en)* | 2024-03-29 | 2024-05-17 | 中国科学院过程工程研究所 | Nucleic acid extraction device |
| CN118185730A (en)* | 2024-03-29 | 2024-06-14 | 中国科学院过程工程研究所 | A nucleic acid extraction system |
| CN118048223B (en)* | 2024-03-29 | 2025-01-17 | 中国科学院过程工程研究所 | Nucleic acid extraction device |
| Publication | Publication Date | Title |
|---|---|---|
| CN110257245A (en) | Nucleic acid detection reagent card | |
| CN110257240B (en) | Nucleic acid extraction, amplification and detection integrated reagent card | |
| US10913060B2 (en) | Loading vials | |
| CN111607506B (en) | Film type nucleic acid amplification micro-fluidic chip and preparation and application methods thereof | |
| US11207655B2 (en) | Microspotting device | |
| CN105658333B (en) | Fluidic Chucks for Nucleic Acid Amplification and Detection | |
| US9662650B2 (en) | Fluidic cartridge and method for processing a liquid sample | |
| CN100503838C (en) | Microfluidic systems for the analysis of nucleic acids | |
| CN101990516B (en) | Multiplex sample preparation system and the use in integrated analysis system thereof | |
| CN105636696B (en) | Pressure reducing valves and valve systems | |
| CN113817601A (en) | Sample treatment and detection device | |
| JP2011517774A (en) | Fluid handling and transfer methods using interconnected multi-chamber devices | |
| CN210506362U (en) | Nucleic acid detection reagent card | |
| CN115537288A (en) | Microfluidic nucleic acid extraction detection box and detection system | |
| CN108300640A (en) | A kind of micro-fluidic chip for nucleic acid automation extraction and detection | |
| CN111321056B (en) | Cartridge device and method for nucleic acid extraction and analysis | |
| CN116099580A (en) | A microfluidic detection device | |
| EP4360758A1 (en) | Cartridge for detecting target analyte | |
| CN221951237U (en) | Microfluidic chip | |
| CN119657250B (en) | Liquid storage type micro-fluidic chip and working method thereof | |
| CN221933959U (en) | A microfluidic chip | |
| CN217838958U (en) | Pathogen detection color development card | |
| US20250144626A1 (en) | Cartridge for detecting target analyte | |
| KR20250065222A (en) | Cartridge for detecting target analyte | |
| CN118685257A (en) | A fully integrated nucleic acid detection chip based on silicon membrane method |
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information | Address after:Room 702, 7 / F, building 2, Room 802, 8 / F, building 3, No. 15, Jinhui Road, Jinsha community, Kengzi street, Pingshan District, Shenzhen City, Guangdong Province Applicant after:Shenzhen Boshi Diagnostic Technology Co.,Ltd. Address before:523000, building 2, building 10, building 202, innovation and Technology Park, Songshan hi tech Industrial Development Zone, Dongguan, Guangdong Applicant before:DONGGUAN BOSH BIOTECHNOLOGIES, Ltd. | |
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication | Application publication date:20190920 |