A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high saltTechnical field
The present invention relates to water treatment field more particularly to a kind of fermented by mixed bacterium for highly concentrated pharmacy waste water with high salt are rawObject preprocess method.
Background technique
Pharmaceutical industry is because its process route is long, raw material is various and reaction is incomplete, conversion rate of products is low, solvent usage amount is bigEtc. factors, the features such as with high salt, highly concentrated, high poison is often presented in waste water, and containing there are many " three cause " class compound, can not biologicallydegraded effluentOr amicine, it is a kind of typical industrial organic waste water difficult to degrade, it is difficult to handle up to standard.In addition, pharmaceutical industry frequently withIntermittence production, and product category changes greatly, and also increases the processing difficulty of pharmacy waste water.
According to statistics, at present pharmaceutical industry can reach its industry Sewage Water Emissions new standard limit value number of the enterprise it is suitableFew, chemical synthesis class, fermentation class, preparation class pharmacy waste water compliance rate are only respectively 9%, 12% and 30%.Due to the spy of waste waterDifferent property, the highest attention by country, society and enterprise, is the generally acknowledged problem of domestic and international sewage treatment for a long time.
Due to highly concentrated pharmacy waste water high poison with high salt, the feature of biodegradability difference, usually it is pre-processed, reduce lifeObject toxicity after improving biodegradability, just can enter the processing of conventional activated sludge system.Currently, common Wastewater Pretreatment meansThere are many, such as the materializations means such as iron-carbon micro-electrolysis, Fenton oxidation, wet oxidation, electro-catalysis, although suitable physico-chemical process founds poleSee shadow, but usually equipment investment is high, energy consumption is high, and easily causes secondary pollution.Therefore it is directed to highly concentrated pharmacy waste water, having mustDevelop the preprocess method of new, highly efficient low cost.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides a kind of mixed bacteria hairs for highly concentrated pharmacy waste water with high saltFerment biologic pretreatment method.For highly concentrated pharmacy waste water with high salt, the present invention selects mixed bacteria versatile, that effect is goodGEMB by organic matter degradation or can be converted into single cell protein by the technique of mixed fermentation, quickly, economically by activated sludgeThe high-concentration waste water with high salt that can not directly handle is converted to the low concentration wastewater with higher biodegradability, compared to other pre- placesReason method cost is lower.
The specific technical proposal of the invention is: a kind of pre- place of fermented by mixed bacterium biology for highly concentrated pharmacy waste water with high saltReason method, the method are adapted to conform with the highly concentrated pharmacy waste water with high salt of following index: biochemical organic carbon Zhan always has in waste waterThe ratio of machine carbon is greater than the ratio > 0.2 of 50%, BOD5/CODcr, and CODcr is in 10000~30000mg/L.
It the described method comprises the following steps:
It the described method comprises the following steps:
S1: select mixed bacteria GEMB as Preliminary fermentation microbial inoculum.
S2: mixed bacteria GEMB is added in waste water, carries out fermentation process.
S3: wastewater fermentation handles outlet water at tail end and accesses MBR film process module, and the suspension thalline that MBR film is retained flows backInto S2.
The effect of S3 is: in practice, it has been found that brine waste density is big, sludge is easy expansion and floats for team of the present invention,Sludge decomposition, therefore microorganism growth is easy to show suspension in high-salt wastewater, but such state is easier to flow with water outletIt loses, therefore biological treatment tank is flowed back into, to compensate and maintain in biological treatment rearmost end access MBR film to retain suspension thallineThe normal concentration of biological treatment tank thallus guarantees water treatment effect.
S4: taking fermentation waste water after system stable operation, microbe genome DNA is extracted, to the microorganism in fermentation waste waterStructure carries out high-flux sequence analysis, determines Pseudomonas ratio and the superiority bacteria spp in final fermentation waste water.
S5: according to S4's as a result, adding growth factor needed for superiority bacteria spp is grown into waste water to optimize pretreating effect.
It is the conventional materialized pretreatment of substitution in Biological Pretreatment system nature, to having certain toxicity, BiostaticWaste water carries out detoxification, improves biodegradability, disposes the reluctant ingredient of routine biochemistry system in waste water, is subsequent routineBiochemistry escorts.Team of the present invention in practice, it has been found that system with runing time growth, due to noxious material in waste waterAccumulation, the reasons such as impact of waste water quality variation can have certain damage to the strain in system, when damage accumulation is to centainlyIt the case where degree just will appear bacterial activity reduction, and treatment effect declines, needs to add strain again.
S6: in operational process, when treatment effeciency reduces, according to the Pseudomonas ratiometric result of S4, by the mixed bacteria of accessThe ratio of each Pseudomonas is adjusted to consistent with Pseudomonas ratio in GEMB.
Team of the present invention finds in early-stage study, the waste water COD cr number of " the density wastewater conditioning tank " of most pharmacy corporationsValue is usually within the scope of 10000mgL to 30000mg/L.In fact, the concentration of such wastewater through organic matter is to be very suitable for microorganismFermentation.Although and complicated components in the waste water of pharmacy corporation, dimethyl formyl amide from waste water (DMF), tetrahydrofuran(THF) etc. the organic solvent that can be utilized by biological decomposition contributes to mainly pollute, as long as selecting suitable mixed bacteria, energyEnough realize the bioanalysis pretreatment of density wastewater.
Biological Pretreatment Techniques are different from conventional activated sludge method, and organic matter and total nitrogen mainly pass through assimilation quiltMicrobial fermentation forms cell body, the discharge system by way of single cell protein, and a small amount of organic matter is disappeared by respirationConsumption.While fermentation, the component such as tetrahydrofuran etc. of " low BOD " is converted into fatty acid, egg by single engineering strain originallyThe ingredient of white matter etc. " high BOD ", not only CODcr is greatly reduced for water outlet, the more notable raising of B/C value, is more suitable for subsequent conventional systemThe processing of system.Although similar from Biological Pretreatment in technique and equipment appearance and conventional activated sludge method, whole systemOxygen supply energy consumption without being calculated according to the removal amount of CODcr in environmental project specification, it is only necessary to designed and advised according to Fermentation EngineeringThe oxygen that model meets fermentation breathing energy supply can (if anaerobic fermentation be then not necessarily to supply oxygen).Water usually per ton removes 10g CODcrComprehensive cost at 0.5 yuan or so." hc effluent " is become using Fermentation Engineering strain the process of " low density wastewater ", not onlyOperating cost is low, and environmental benefit is obvious, not will form secondary pollution.
Preferably, in S1, the composition of the mixed bacteria GEMB are as follows: the Halomonas of 30-40%, 26-30%'sThe Clostridium of the Oligella of the Desulfurispirillum of Bacillus, 10-14%, 13-17%, 3-7%, 2-The %Paenibacillus of 4% Idiomarina, 1-3.
Team of the present invention divides bacterium to obtain nearly 40,000 plants of bacterium in early period from ocean, glacier, hot spring, salt alkali lake, sour lake and other placesKind, wherein the resistance strain containing the characteristics such as salt tolerant, acidproof, alkaline-resisting, anti-medicine, anti-solvent, high temperature resistant, low temperature resistant, comprising bacterium,Fungi, actinomyces after many experiments, are being finally obtained above-mentioned with high salt, You Jirong suitable for medicine producing wastewaterThe effective complex bacterium kind GEMB of agent and important doctorization Organic Ingredients.The mixed bacteria GEMB of above-mentioned composition is versatile, and effect is good,By organic matter degradation or it is converted into single cell protein by the technique of mixed fermentation, quickly, economically can not be straight by activated sludgeThe high-concentration waste water for connecing processing is converted to the low concentration wastewater with higher biodegradability, greatly reduces subsequent routine biochemistry systemProcessing load, can substantially cope with the highly concentrated pharmacy waste water with high salt for meeting water quality indicator of the present invention.
Preferably, S2 method particularly includes: waste water is pumped into water quality adjustment pond, urea and microcosmic salt is added and mixes equalIt is even；Waste water leads to after water quality adjustment in the biological treatment tank of tandem, and 15-25w/v% has been added in biological treatment tankMixed bacteria GEMB；CDO=5000-10000mg/L underload intermittent water inflow, controlled DO and existed in 2-5mg/L, temperature early period25-30℃；To in biological treatment tank cell concentration stablize, thallus 40% or more is reached to the removal rate of waste water COD cr after again intoRow CDO=10000-30000mg/L high load capacity continuum micromeehanics, into the operation control stage；In this stage daily to water inlet and lifeCOD, NH of object processing pond water outlet3- N index is monitored, to detect Biological Pretreatment effect, until the removal of waste water COD crRate is 50% or more.
Elder generation's underload intermittent water inflow of the present invention, the later period again high load capacity continuum micromeehanics the reason of be: the mixing initially addedThe ratio of each Pseudomonas is a general ratio in strain GEMB, although there is universality, for specific waste water certainly notIt is best proportion, therefore the effect of initial underload intermittent water inflow is bacteria, strain can be allowed gradually to adapt to waste water, play domesticationEffect.Continuum micromeehanics are carried out after stablizing.
Preferably, in S2, add urea and microcosmic salt make in waste water C: N: P molar ratio=(190-210): (4-6):1。
Preferably, the bottom of biological treatment tank is equipped with aerating system, and waste water bottom in biological treatment tank in S2Water inlet, top water outlet；Biological treatment tank side wall is equipped with mud discharging mouth.
Bottom water inlet, bottom in and top out can play the role of good mixed flow, the upward percussion of lower part water inlet and aerationThallus can reach suspended state in system when downward settlement action reaches balance with thallus, and treatment effect is best.
Preferably, in the underload intermittent water inflow stage, the brown sugar of 0.8-1.2kg/ tons of water is added into waste water in S2,Stop adding after into the high load capacity continuum micromeehanics stage.
Brown sugar is equivalent to carbon source, can promote thallus fast-growth, plays the role of rapid amplifying strain.
Preferably, using Pvdf Microporous Hollow Fiber Membrane, membrane aperture is 0.1~0.5 micron in S3.
Preferably, the superiority bacteria spp is ratio ranking near several preceding Pseudomonas, the abundance of superiority bacteria spp in S4Summation is more than 50%.
Abundance is set as more than 50%, the reason is that the strain to play a major role can be regarded as under the abundance.
Preferably, the growth factor is selected from vitamin, microelement and biotin in S5.
Preferably, in S6, mark that the treatment effeciency reduces are as follows: 40% is lower than to the removal rate of waste water COD cr, orThe 50% of cell concentration when cell concentration is lower than normal treatment effeciency in microscopy zymocyte liquid.
It is compared with the prior art, the beneficial effects of the present invention are:
For highly concentrated pharmacy waste water with high salt, the present invention filters out that versatile, effect is good from the strain library that many years scientific research is accumulatedMixed bacteria GEMB, single cell protein by organic matter degradation or can be converted by the technique of mixed fermentation, quickly, economicallyThe high-concentration waste water with high salt that activated sludge can not be handled directly is converted to the low concentration wastewater with higher biodegradability, biochemicalProperty can be improved 3-8 times, and remove the COD and ammonia nitrogen of 50-90% in sewage, reduce the processing load of subsequent routine biochemistry system about50-90%.
Detailed description of the invention
Fig. 1 is wastewater treatment front and back nearly two weeks degradation situations of COD in embodiment 1；
Fig. 2 is wastewater treatment front and back nearly two weeks degradation situations of ammonia nitrogen in embodiment 1.
Fig. 3 is S4 fermentation waste water detection gained Pseudomonas ratio in embodiment 1.
Specific embodiment
The present invention will be further described with reference to the examples below.
Total embodiment
A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt, the method be adapted to conform withThe highly concentrated pharmacy waste water with high salt of lower index: the ratio that biochemical organic carbon accounts for total organic carbon in waste water is greater than 50%, BOD5/The ratio > 0.2 of CODcr, CODcr is in 10000~30000mg/L.
It the described method comprises the following steps:
S1: select mixed bacteria GEMB as Preliminary fermentation microbial inoculum.The composition of the mixed bacteria GEMB are as follows: 30-40%'sThe Oligella of the Desulfurispirillum of the Bacillus of Halomonas, 26-30%, 10-14%, 13-17%, 3-The %Paenibacillus of the Idiomarina of 7% Clostridium, 2-4%, 1-3.
S2: being pumped into water quality adjustment pond for waste water, and adding urea and microcosmic salt makes molar ratio=(190- of C:N:P in waste water210): (4-6): 1, it is uniformly mixed；Waste water leads to after water quality adjustment in the biological treatment tank of tandem, biological treatment tankIn add the mixed bacteria GEMB of 15-25w/v%；Early period, CDO=5000-10000mg/L underload intermittent water inflow, controlled DOIn 2-5mg/L, temperature at 25-30 DEG C；Stablize to cell concentration in biological treatment tank, thallus reaches the removal rate of waste water COD crCDO=10000-30000mg/L high load capacity continuum micromeehanics are carried out after to 40% or more again, into the operation control stage；In this rankSection is daily to COD, NH of water inlet and biological treatment tank water outlet3- N index is monitored, to detect Biological Pretreatment effect, untilThe removal rate of waste water COD cr is 50% or more.
Preferably, the bottom of biological treatment tank is equipped with aerating system, and waste water bottom in biological treatment tank in S2Water inlet, top water outlet；Biological treatment tank side wall is equipped with mud discharging mouth.
Preferably, in the underload intermittent water inflow stage, the brown sugar of 0.8-1.2kg/ tons of water is added into waste water in S2,Stop adding after into the high load capacity continuum micromeehanics stage.
S3: carrying out MBR film (Pvdf Microporous Hollow Fiber Membrane, membrane aperture are 0.1~0.5 micron) processing to waste water, andThe suspension thalline that MBR film retains is back in S2.
S4: taking fermentation waste water after system stable operation, microbe genome DNA is extracted, to the microorganism in fermentation waste waterStructure carries out high-flux sequence analysis, determines Pseudomonas ratio and the superiority bacteria spp in final fermentation waste water；The superiority bacteria spp isRatio ranking is more than 50% near several preceding Pseudomonas, the abundance summation of superiority bacteria spp.
S5: according to S4's as a result, adding growth factor needed for superiority bacteria spp is grown into waste water to optimize pretreating effect.The growth factor is selected from vitamin, microelement and biotin etc..
S6: in operational process, when treatment effeciency reduces, according to the Pseudomonas ratiometric result of S4, by the mixed bacteria of accessThe ratio of each Pseudomonas is adjusted to consistent with Pseudomonas ratio in GEMB.The mark that the treatment effeciency reduces are as follows: to waste water COD cr'sRemoval rate is lower than 50% of cell concentration when cell concentration is lower than normal treatment effeciency in 40% or microscopy zymocyte liquid.
Embodiment 1: the case history of the Biological Pretreatment of the cephalo pharmacy waste water hc effluent of Zhejiang enterprise.
Project name: the Biological Pretreatment of cephalo pharmacy waste water
Waste water quality analysis: high-concentration waste water contains noxious material difficult to degrade such as dimethylformamide (DMF), tetrahydrofuran(THF), pyridine, antibiotic etc., such water can not be directly entered the existing biochemical treatment system of enterprise, need and low concentration wastewaterMixed diluting can just be continued with to COD lower than 8000mg/L, therefore the production capacity of enterprise is limited in environmental protection.Water quality substantiallyInformation is as follows:
| Water quality indicator | COD | NH3-N | TP | TDS | pH |
| Content | 25000(mg/L) | 450(mg/L) | 2.5(mg/L) | 3% | 6 |
By analyzing each workshop leading portion production technology and sewage collecting rule and combining waste water mass spectrometric data, technical team is recognizedAlthough contain antibiotic, high-sulfate, and organic concentration height for the waste water, but still there is the feasibility of fermentation.
Fermentation process designs: according to the physiological characteristic of mixed bacteria, designing corresponding zymotechnique, thallus separating technology withAnd matched facilities and equipment.Hc effluent is finally directly carried out to the Biological Pretreatment of mixed bacteria.
Method particularly includes:
S1: select mixed bacteria GEMB as Preliminary fermentation microbial inoculum.The composition of the mixed bacteria GEMB are as follows: 35%Halomonas, 28% Bacillus, 12% Desulfurispirillum, 15% Oligella, 5%Clostridium, 3% Idiomarina, 2 %Paenibacillus.
S2: being pumped into water quality adjustment pond for waste water, add urea and microcosmic salt make in waste water C: N: P molar ratio=200: 5: 1,It is uniformly mixed；Waste water leads to after water quality adjustment in the biological treatment tank of tandem, and 20% (w/v) is pressed in biological treatment tankConnect bacterium than be added mixed bacteria GEMB；3 times of bio-chemical effluent dilution of waste water early period, being diluted to CODcr is 8000mg/L or soUnderload intermittent water inflow, control DO in 4mg/L or so, temperature at 28 DEG C；Stablize to cell concentration in biological treatment tank, thallusCarry out COD=25000mg/L high load capacity raw water after reaching 40% or more to the removal rate of waste water COD cr again, and keep continuously intoWater outlet, into the operation control stage；In this stage daily to COD, NH of water inlet and biological treatment tank water outlet3- N index is supervisedIt surveys, to detect Biological Pretreatment effect, until the removal rate of waste water COD cr is 50% or more.
Wherein, the bottom of biological treatment tank is equipped with aerating system, and waste water bottom in biological treatment tank intakes, and top goes outWater；Biological treatment tank side wall is equipped with mud discharging mouth.In the underload intermittent water inflow stage, the red of 1.0kg/ tons of water is added into waste waterSugar, into the high load capacity continuum micromeehanics stage after stop adding.
S3: wastewater fermentation handles outlet water at tail end and accesses MBR film process module, carries out MBR film (polyvinylidene fluoride hollow fiberFilm, membrane aperture are 0.2 micron) processing, and the suspension thalline that MBR film retains is back in S2.
S4: taking fermentation waste water after system stable operation, microbe genome DNA is extracted, to the microorganism in fermentation waste waterStructure carries out high-flux sequence analysis, determines that the Pseudomonas ratio and superiority bacteria spp in final fermentation waste water are shown in Fig. 3；The dominant bacteriaBelong to be ratio ranking near preceding 5 Pseudomonas Bacillus (43.98), Halomonas (32.13), Oligella (11.22),Desulfurispirillum (4.35), Paenibacillus (3.77), the abundance summation of superiority bacteria spp are more than 50%.
S5: according to S4's as a result, additionally adding growth factor needed for superiority bacteria spp is grown into waste water: trace element cobalt,Molybdenum, copper, manganese and biotin are to optimize pretreating effect.
S6: in operational process, when treatment effeciency reduces, according to the Pseudomonas ratiometric result of S4, by the mixed bacteria of accessThe ratio of each Pseudomonas is adjusted to consistent with Pseudomonas ratio in GEMB.The mark that the treatment effeciency reduces are as follows: to waste water COD cr'sRemoval rate is lower than 50% of cell concentration when cell concentration is lower than normal treatment effeciency in 40% or microscopy zymocyte liquid.
Main indicator is as follows after processing: whole a couple of days residence time, as shown in Figure 1, Figure 2 (abscissa is runing time in figure,It, ordinate is mg/L) shown in, water-quality COD cr removal rate is up to 50% or more, and nitrogen removal rate is up to 80% or more, and systemRun highly stable, fluctuation is small, greatly reduces follow-up system burden, proposes the ability of entire sewage plant processing hc effluentRise 40%.
Embodiment 2
S1: select mixed bacteria GEMB as Preliminary fermentation microbial inoculum.The composition of the mixed bacteria GEMB are as follows: 30%Halomonas, 30% Bacillus, 10% Desulfurispirillum, 17% Oligella, 7%Clostridium, 4% Idiomarina, 2 %Paenibacillus.
S2: being pumped into water quality adjustment pond for waste water, add urea and microcosmic salt make in waste water C: N: P molar ratio=190: 4: 1,It is uniformly mixed；Waste water leads to after water quality adjustment in the biological treatment tank of tandem, and 15w/ has been added in biological treatment tankThe mixed bacteria GEMB of v%；Early period, CDO=5000mg/L underload intermittent water inflow, controlled DO in 2mg/L, temperature at 25 DEG C；ToCell concentration is stablized in biological treatment tank, and thallus carries out CDO=after reaching 40% or more to the removal rate of waste water COD cr again10000mg/L high load capacity continuum micromeehanics, into the operation control stage；Water inlet and biological treatment tank are discharged daily in this stageCOD、NH3- N index is monitored, to detect Biological Pretreatment effect, until the removal rate of waste water COD cr is 50% or more.
Wherein, the bottom of biological treatment tank is equipped with aerating system, and waste water bottom in biological treatment tank intakes, and top goes outWater；Biological treatment tank side wall is equipped with mud discharging mouth.In the underload intermittent water inflow stage, the red of 0.8kg/ tons of water is added into waste waterSugar, into the high load capacity continuum micromeehanics stage after stop adding.
S3: wastewater fermentation handles outlet water at tail end and accesses MBR film process module, carries out MBR film (polyvinylidene fluoride hollow fiberFilm, membrane aperture are 0.2 micron) processing, and the suspension thalline that MBR film retains is back in S2.
S4: taking fermentation waste water after system stable operation, microbe genome DNA is extracted, to the microorganism in fermentation waste waterStructure carries out high-flux sequence analysis, determines Pseudomonas ratio and the superiority bacteria spp in final fermentation waste water；The superiority bacteria spp isRatio ranking is more than 50% near several preceding Pseudomonas, the abundance summation of superiority bacteria spp.
S5: according to S4's as a result, adding growth factor needed for superiority bacteria spp is grown into waste water to optimize pretreating effect.The growth factor is selected from vitamin, microelement and biotin.
S6: in operational process, when treatment effeciency reduces, according to the Pseudomonas ratiometric result of S4, by the mixed bacteria of accessThe ratio of each Pseudomonas is adjusted to consistent with Pseudomonas ratio in GEMB.The mark that the treatment effeciency reduces are as follows: to waste water COD cr'sRemoval rate is lower than 50% of cell concentration when cell concentration is lower than normal treatment effeciency in 40% or microscopy zymocyte liquid.
Embodiment 3
S1: select mixed bacteria GEMB as Preliminary fermentation microbial inoculum.The composition of the mixed bacteria GEMB are as follows: 40%Halomonas, 26% Bacillus, 14% Desulfurispirillum, 13% Oligella, 3%Clostridium, 2% Idiomarina, 2 %Paenibacillus.
S2: being pumped into water quality adjustment pond for waste water, add urea and microcosmic salt make in waste water C: N: P molar ratio=210: 6: 1,It is uniformly mixed；Waste water leads to after water quality adjustment in the biological treatment tank of tandem, and 15w/ has been added in biological treatment tankThe mixed bacteria GEMB of v%；Early period, CDO=10000mg/L underload intermittent water inflow, controlled DO in 5mg/L, temperature at 30 DEG C；Stablize to cell concentration in biological treatment tank, thallus carries out CDO=after reaching 40% or more to the removal rate of waste water COD cr again30000mg/L high load capacity continuum micromeehanics, into the operation control stage；Water inlet and biological treatment tank are discharged daily in this stageCOD、NH3- N index is monitored, to detect Biological Pretreatment effect, until the removal rate of waste water COD cr is 50% or more.
Wherein, the bottom of biological treatment tank is equipped with aerating system, and waste water bottom in biological treatment tank intakes, and top goes outWater；Biological treatment tank side wall is equipped with mud discharging mouth.In the underload intermittent water inflow stage, the red of 1.2kg/ tons of water is added into waste waterSugar, into the high load capacity continuum micromeehanics stage after stop adding.
S3: wastewater fermentation handles outlet water at tail end and accesses MBR film process module, carries out MBR film (polyvinylidene fluoride hollow fiberFilm, membrane aperture are 0.2 micron) processing, and the suspension thalline that MBR film retains is back in S2.
S4: taking fermentation waste water after system stable operation, microbe genome DNA is extracted, to the microorganism in fermentation waste waterStructure carries out high-flux sequence analysis, determines Pseudomonas ratio and the superiority bacteria spp in final fermentation waste water；The superiority bacteria spp isRatio ranking is more than 50% near several preceding Pseudomonas, the abundance summation of superiority bacteria spp.
S5: according to S4's as a result, adding growth factor needed for superiority bacteria spp is grown into waste water to optimize pretreating effect.The growth factor is selected from vitamin, microelement and biotin.
S6: in operational process, when treatment effeciency reduces, according to the Pseudomonas ratiometric result of S4, by the mixed bacteria of accessThe ratio of each Pseudomonas is adjusted to consistent with Pseudomonas ratio in GEMB.The mark that the treatment effeciency reduces are as follows: to waste water COD cr'sRemoval rate is lower than 50% of cell concentration when cell concentration is lower than normal treatment effeciency in 40% or microscopy zymocyte liquid.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present inventionMethod therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not intended to limit the invention in any way, it is all according to the present inventionTechnical spirit any simple modification, change and equivalent transformation to the above embodiments, still fall within the technology of the present invention sideThe protection scope of case.