技术领域technical field
本发明涉及医学检测技术领域，具体来说是一种全自动支原体培养加样检测仪及其培养加样检测方法。The invention relates to the technical field of medical detection, in particular to a fully automatic mycoplasma culture and sample addition detector and a culture and sample addition detection method thereof.
背景技术Background technique
目前，各医疗机构实验室均采用手工方法进行支原体培养的加样和检测。手工法具体是先手动采集标本，然后选取培养瓶，用吸头从培养瓶中吸取具体数值的培养液，再将标本溶如培养瓶，用吸头吸取具体数值的加入了标本的培养液，将二者放入培养区进行培养。由于加入标本前后的培养液要进行对比，所以前后吸取的培养液量是一致的，手动吸取培养液难以做到精确，同时手工法还存在着效率低、个体差异大和错误率高等诸多不足之处。At present, the laboratories of various medical institutions use manual methods to add samples and detect mycoplasma culture. The manual method is to collect the specimen manually first, then select the culture bottle, use the tip to absorb the culture solution of specific value from the culture bottle, then dissolve the sample into the culture bottle, and use the tip to absorb the culture solution with the specific value added to the sample, Put the two into the cultivation area for cultivation. Since the culture solution before and after adding the specimen should be compared, the amount of culture solution drawn before and after is the same. It is difficult to draw the culture solution accurately manually. At the same time, the manual method also has many shortcomings such as low efficiency, large individual differences, and high error rate. .
发明内容Contents of the invention
本发明的目的在于解决现有技术的不足，提供一种结构简单，精度高的全自动支原体培养加样检测仪的技术方案和培养加样方法。The purpose of the present invention is to solve the deficiencies of the prior art, and provide a technical scheme and a method for culturing and adding samples of a fully automatic mycoplasma culture and sample detection instrument with simple structure and high precision.
为了实现上述目的，设计一种全自动支原体培养加样检测仪，包括耗材储存区、工作区、培养区、结果判读区，其特征在于：In order to achieve the above purpose, a fully automatic mycoplasma culture and sample detection instrument is designed, including a consumable storage area, a work area, a culture area, and a result interpretation area, which is characterized in that:
所述耗材储存区内设有培养瓶和培养板；Culture bottles and culture plates are arranged in the consumables storage area;
所述工作区底部设有工作台，工作台上方设有机械臂和吸头23，工作台一侧设有标本架17，标本架17内设有试管，试管内设有标本采集拭子，试管顶部设有标本采集拭子体卡43，标本采集拭子体卡43与设置在标本架17上部的标本夹凹卡49相配合，试管上还设有标本采集拭子头卡41，标本采集拭子头卡41底部连接标本采集拭子杆。The bottom of the work area is provided with a workbench, the top of the workbench is provided with a mechanical arm and a suction head 23, and one side of the workbench is provided with a specimen rack 17, and a test tube is arranged in the specimen rack 17, and a specimen collection swab is arranged in the test tube. The top is provided with a sample collection swab body card 43, which cooperates with the sample holder concave card 49 arranged on the top of the sample holder 17, and the test tube is also provided with a sample collection swab head card 41, and the sample collection swab head card 41 is arranged on the test tube. The bottom of the sub-head card 41 is connected to the sample collection swab bar.
所述的一种全自动支原体培养加样检测仪，其特征在于：所述的机械臂通过升降装置与导轨21连接，导轨21能使机械臂前后左右移动，升降装置能使机械臂上下移动，机械臂机头能进行旋转；所述的吸头23通过升降装置与导轨21连接，导轨21能使吸头23前后左右移动，升降装置能使吸头23上下移动。The full-automatic mycoplasma culture and sampling detection instrument is characterized in that: the mechanical arm is connected with the guide rail 21 through the lifting device, the guide rail 21 can make the mechanical arm move back and forth, left and right, and the lifting device can make the mechanical arm move up and down, The head of the mechanical arm can rotate; the suction head 23 is connected with the guide rail 21 through the lifting device, the guide rail 21 can make the suction head 23 move back and forth, left and right, and the lifting device can make the suction head 23 move up and down.
所述的一种全自动支原体培养加样检测仪，其特征在于：所述工作台一侧设有培养瓶放置处13和石蜡油瓶放置处31。The full-automatic mycoplasma culture and sampling detection instrument is characterized in that: a culture bottle placement place 13 and a paraffin oil bottle placement place 31 are provided on one side of the workbench.
所述的一种全自动支原体培养加样检测仪，其特征在于：所述的结果判读区7内设有摄像头4。The above-mentioned fully automatic mycoplasma culture and sampling detection instrument is characterized in that: a camera 4 is provided in the result interpretation area 7 .
所述的一种全自动支原体培养加样检测仪，其特征在于：所述的培养瓶包括培养瓶盖（52）和培养瓶底（55），培养瓶盖52和培养瓶底55均为多边形，所述的石蜡油瓶盖70和石蜡油瓶底72均为多边形。The full-automatic mycoplasma culture and sample detection instrument is characterized in that: the culture bottle includes a culture bottle cap (52) and a culture bottle bottom (55), and both the culture bottle cap 52 and the culture bottle bottom 55 are polygonal , the paraffin oil bottle cap 70 and the paraffin oil bottle bottom 72 are polygonal.
所述的一种全自动支原体培养加样检测仪，其特征在于：所述的标本采集拭子、培养瓶和培养板上设有条形码。The full-automatic mycoplasma culture and sampling detection instrument is characterized in that barcodes are set on the specimen collection swabs, culture bottles and culture plates.
所述的全自动支原体培养加样检测仪，其特征在于：所述的培养区设有培养箱，培养箱包括培养板培养箱门61和培养箱加热板63，培养箱门61受力自动内开，培养箱内设有培养箱加热板63以实现独立培养。The full-automatic mycoplasma culture and sampling detection instrument is characterized in that: the culture area is provided with an incubator, and the incubator includes a culture plate incubator door 61 and an incubator heating plate 63, and the incubator door 61 is automatically internalized under force. Open, an incubator heating plate 63 is provided in the incubator to realize independent cultivation.
所述的全自动支原体培养加样检测仪，其特征在于：所述培养瓶分男性标本用培养瓶和女性标本用培养瓶，男性标本用培养瓶上设有男性标本用培养瓶标本取刮器35，女性标本用培养瓶上设有女性标本用培养瓶标本取刮器54，男性标本采集拭子48在男性标本用培养瓶标本取刮器35内上下摩擦并旋转，以便将标本充分溶于培养液中，女性标本采集拭子46在女性标本用培养瓶标本取刮器54内上下摩擦并旋转，以便将标本充分溶于培养液中。The full-automatic mycoplasma culture and sampling detection instrument is characterized in that: the culture bottle is divided into a culture bottle for male specimens and a culture bottle for female specimens, and the culture bottle for male specimens is equipped with a culture bottle specimen scraper for male specimens 35. The culture bottle for female specimens is provided with a scraper 54 for female specimens, and the male specimen collection swab 48 rubs and rotates up and down in the scraper 35 for male specimens, so that the specimens are fully dissolved in In the culture solution, the female sample collection swab 46 rubs and rotates up and down in the sample scraper 54 of the culture bottle for female samples, so as to fully dissolve the sample in the culture solution.
所述的全自动支原体培养加样检测仪，其特征在于，所述培养瓶放置处13在溶解标本后可自动振动，用于混匀标本。The automatic mycoplasma culture and sampling detection instrument is characterized in that the place 13 for placing the culture bottle can vibrate automatically after dissolving the specimen to mix the specimen evenly.
所述全自动支原体培养加样检测仪的培养加样检测方法，其特征在于：The culture and sample detection method of the fully automatic mycoplasma culture and sample detection instrument is characterized in that:
第一步：用标本采集拭子采集标本；The first step: collect the specimen with the specimen collection swab;
第二步：将标本采集拭子体卡卡在标本架凹卡内备用，条码扫描器读取条码信息；Step 2: Put the sample collection swab body card in the concave card of the sample rack for standby, and the barcode scanner reads the barcode information;
第三步：第二机械臂24从培养瓶储存室30抓取对应标本数量的培养瓶置于操作台15培养瓶放置处13备用，第二机械臂24从培养板储存室25抓取对应标本数量的培养板置于操作台15培养板放置处28备用；Step 3: The second mechanical arm 24 grabs the culture bottles corresponding to the number of specimens from the culture bottle storage room 30 and places them in the operation table 15 at the culture bottle placement place 13 for standby, and the second robot arm 24 grabs the corresponding samples from the culture plate storage room 25 A number of culture plates are placed in the operating table 15 culture plate placement place 28 for subsequent use;
第四步：条码扫描器29读取培养瓶条码53和培养板条码58，通过条码指定并记录标本与对应的培养瓶和培养板，确保信息和结果正确传输；Step 4: The barcode scanner 29 reads the barcode 53 of the culture bottle and the barcode 58 of the culture plate, specifies and records the specimen and the corresponding culture bottle and culture plate through the barcode, to ensure the correct transmission of information and results;
第五步：第一机械臂8打开培养板盖板56并翻转置于操作台15，第二机械臂24拧开培养瓶盖52并放在翻转的培养板盖板56上；Step 5: The first robotic arm 8 opens the culture plate cover 56 and turns it over and places it on the console 15. The second robotic arm 24 unscrews the culture bottle cap 52 and places it on the turned culture plate cover 56;
第六步：无菌吸头吸取无菌培养液置于培养板阴性质控孔内；Step 6: Take the sterile culture solution with a sterile tip and place it in the negative quality control well of the culture plate;
第七步：条码扫描器29读取标本采集拭子条码44信息；Step 7: The barcode scanner 29 reads the barcode 44 information of the sample collection swab;
第八步：机械臂24抓着标本采集拭子，将标本采集拭子48在其对应的培养瓶中用培养瓶标本取刮器35上下摩擦并旋转以将标本充分溶于培养液中，再将标本插回对应的试管内；Step 8: The mechanical arm 24 grasps the specimen collection swab, rubs the specimen collection swab 48 in its corresponding culture bottle with the culture bottle specimen scraper 35 and rotates it up and down to fully dissolve the specimen in the culture solution, and then Insert the specimen back into the corresponding test tube;
第九步：吸头23将混有标本的培养液加入培养板剩下的培养孔中；Step 9: Add the culture solution mixed with the specimen into the remaining culture wells of the culture plate with the suction head 23;
第十步：将吸头打入废物盒12内；Step 10: Put the suction head into the waste box 12;
第十一步：第二机械臂24拧紧培养瓶盖52并将培养瓶放入培养瓶培养箱16中进行培养；Step 11: the second mechanical arm 24 tightens the cap 52 of the culture bottle and puts the culture bottle into the culture bottle incubator 16 for cultivation;
第十二步：第二机械臂24将石蜡油瓶盖打开，吸头从石蜡油瓶中吸取石蜡油依次加入培养板各培养孔中；Step 12: the second mechanical arm 24 opens the cap of the paraffin oil bottle, and the suction head absorbs the paraffin oil from the paraffin oil bottle and adds it to each culture well of the culture plate in turn;
第十三步：第一机械臂8将培养板盖板盖56好并将培养板送入相应的培养仓中；Step 13: The first robotic arm 8 covers the culture plate cover 56 and sends the culture plate into the corresponding culture bin;
第十四步：第一机械臂8将培养板抓出并置于结果判读区7进行第一次结果判读并由摄像头4拍照存档，再将培养板送入培养仓继续培养；Step 14: The first robotic arm 8 grabs the culture plate and places it in the result interpretation area 7 for the first result interpretation, and the camera 4 takes pictures for archiving, and then sends the culture plate into the culture chamber to continue culturing;
第十五步：第一机械臂8将培养板抓出并置于结果判读区7进行第二次结果判读并由摄像头4拍照存档，结合两次结果将最终结果经信号线路3传入LIS系统待审；Step 15: The first robotic arm 8 grabs the culture plate and places it in the result interpretation area 7 for the second result interpretation, and the camera 4 takes pictures for archiving, and combines the two results to transmit the final result to the LIS system through the signal line 3 Pending;
第十六步：将培养结束并发送结果的培养板和培养瓶放入废物盒12内，结束一批标本的培养。Step 16: Put the culture plates and culture bottles that have been cultured and sent results into the waste box 12, and the culture of a batch of specimens is ended.
本发明同现有技术相比，具有如下优点：Compared with the prior art, the present invention has the following advantages:
1. 能够实现支原体培养操作全自动。1. It can realize the automatic operation of mycoplasma culture.
2. 仪器自动操作，结果重复性好，提高工作效率。2. The instrument is operated automatically, the result is repeatable, and the work efficiency is improved.
3. 单个培养，提高检测质量。3. Single culture, improve detection quality.
4. 仪器自动判读结果，减少人为错误。4. The instrument automatically interprets the results to reduce human errors.
附图说明Description of drawings
图1是本发明的整体结构示意图；Fig. 1 is the overall structural representation of the present invention;
图2是本发明男性标本采集拭子的示意图；Fig. 2 is the schematic diagram of male sample collection swab of the present invention;
图3是本发明女性标本采集拭子的示意图；Fig. 3 is the schematic diagram of swab of female sample collection of the present invention;
图4是本发明标本架示意图；Fig. 4 is a schematic diagram of a specimen holder of the present invention;
图5是本发明男性培养瓶示意图；Fig. 5 is a schematic diagram of a male culture bottle of the present invention;
图6是本发明女性培养瓶示意图；Fig. 6 is a schematic diagram of a female culture bottle of the present invention;
图7是本发明培养板示意图；Fig. 7 is a schematic diagram of the culture plate of the present invention;
图8是本发明培养板上条形码示意图；Fig. 8 is a schematic diagram of the barcode on the culture plate of the present invention;
图9是本发明培养板和培养瓶的培养箱的主视图；Fig. 9 is the front view of the incubator of culture plate and culture bottle of the present invention;
图10是本发明培养板的培养箱的平面图；Fig. 10 is the plan view of the incubator of culture plate of the present invention;
图11是本发明培养瓶的培养箱的平面图；Fig. 11 is the plan view of the incubator of culture bottle of the present invention;
图12是本发明培养瓶上条形码的示意图；Figure 12 is a schematic diagram of the barcode on the culture bottle of the present invention;
图13是本发明培养板和培养瓶的储存箱的主视图；Fig. 13 is the front view of the storage box of culture plate and culture bottle of the present invention;
图14是本发明培养板的储存箱的平面图；Fig. 14 is the plan view of the storage box of culture plate of the present invention;
图15是本发明培养瓶的储存箱的平面图；Fig. 15 is a plan view of a storage box of a culture bottle of the present invention;
图16是本发明石蜡油瓶的示意图；Fig. 16 is the schematic diagram of paraffin oil bottle of the present invention;
图中：1.控制室 2.仪器开关 3.电源和信号线路 4.摄像头 5.光源灯管 6.结果判读区门 7.结果判读区 8.第一机械臂 9.废物盒门 10.废物盒拉手 11.培养板和培养箱培养仓 12.废物盒 13.培养瓶放置处 14.培养板盖板放置处 15.操作台 16.培养瓶培养箱 17.标本架 18.仪器底座 19.紫外灯 20.机械臂轨道 21.导轨 22.操作间门 23.吸头 24.第二机械臂 25.培养板储存室 26.无菌吸头盒 27.吸头盒28.培养板放置处 29.条码扫描器 30.培养瓶储存室 31.石蜡油瓶放置处 32.培养板和培养瓶储存室制冷装置 33.培养板和培养瓶储存室温度调节和显示器 34.培养板和培养瓶培养箱温度调节和显示器 35.男性标本用培养瓶标本取刮器 41.标本采集拭子头卡 42.标本采集拭子颈 43.标本采集拭子体卡 44.标本采集拭子条形码 45.女性标本采集拭子杆 46.女性标本采集拭子 47.男性标本采集拭子杆 48.男性标本采集拭子 49.标本架凹卡 50.标本抓手凹卡 51.机械臂滑杆 52.培养瓶盖 53.培养瓶条形码 54.女性标本用培养瓶标本取刮器 55.培养瓶底 56.培养板盖 57.培养板加样孔58.培养板条形码 59.培养瓶储存条培养瓶放置孔 60.培养瓶储存条条形码 61.培养板培养箱门 62.培养瓶培养仓门 63.培养箱加热板 64.培养瓶培养条条形码 65.培养瓶培养条培养瓶放置孔 66.培养板储存箱门 67.培养瓶储存仓门 68.培养板 69.培养瓶培养条 70.石蜡油瓶盖 71.石蜡油瓶条码 72.石蜡油瓶底。In the figure: 1. Control room 2. Instrument switch 3. Power supply and signal line 4. Camera 5. Light source lamp 6. Result interpretation area door 7. Result interpretation area 8. First mechanical arm 9. Waste box door 10. Waste Box handle 11. Culture plate and incubator culture compartment 12. Waste box 13. Place for culture bottle 14. Place for culture plate cover 15. Operating table 16. Culture bottle incubator 17. Specimen rack 18. Instrument base 19. Ultraviolet Light 20. Robotic arm track 21. Guide rail 22. Operating room door 23. Suction head 24. Second robotic arm 25. Culture plate storage room 26. Sterile tip box 27. Tip box 28. Culture plate placement place 29. Barcode scanner 30. Culture bottle storage room 31. Paraffin oil bottle placement place 32. Culture plate and culture bottle storage room refrigeration device 33. Culture plate and culture bottle storage room temperature adjustment and display 34. Culture plate and culture bottle incubator temperature Adjustment and display 35. Culture bottle specimen scraper for male specimens 41. Specimen collection swab head card 42. Specimen collection swab neck 43. Specimen collection swab body card 44. Specimen collection swab barcode 45. Female specimen collection swab Subpole 46. Female specimen collection swab 47. Male specimen collection swab rod 48. Male specimen collection swab 49. Specimen holder concave card 50. Specimen gripper concave card 51. Mechanical arm slide bar 52. Culture bottle cap 53. Culture bottle bar code 54. Culture bottle specimen scraper for female specimens 55. Culture bottle bottom 56. Culture plate cover 57. Culture plate sample hole 58. Culture plate bar code 59. Culture bottle storage strip Culture bottle placement hole 60. Culture bottle Storage bar code 61. Culture plate incubator door 62. Culture bottle culture bin door 63. Incubator heating plate 64. Culture bottle culture bar bar code 65. Culture bottle culture bar culture bottle placement hole 66. Culture plate storage box door 67. Culture Bottle storage door 68. Culture plate 69. Culture bottle culture bar 70. Paraffin oil bottle cap 71. Paraffin oil bottle bar code 72. Paraffin oil bottle bottom.
具体实施方式Detailed ways
结合附图对本技术方案做进一步的解释。The technical solution is further explained in conjunction with the accompanying drawings.
实施例一：参见图1Embodiment 1: See Figure 1
包括耗材储存区、工作区、培养区、结果判读区， 耗材储存区内设有培养瓶和培养板；工作区底部设有工作台，工作台上方设有机械臂和吸头23，机械臂通过升降装置与导轨21连接，导轨21能使机械臂前后左右移动，升降装置能使机械臂上下移动，机械臂机头能进行旋转；吸头23通过升降装置与导轨21连接，导轨21能使吸头23前后左右移动，升降装置能使吸头23上下移动，工作台一侧设有标本架17，标本架17内设有试管，试管顶部设有标本采集拭子体卡43，标本采集拭子体卡43与设置在标本架17上部的标本夹凹卡49相配合，试管上还设有标本采集拭子头卡41，标本采集拭子头卡41底部连接标本采集拭子杆，工作台另一侧设有培养瓶放置处13和石蜡油瓶放置处31，结果判读区7内设有摄像头4。Including the consumables storage area, work area, cultivation area, and result interpretation area, there are culture bottles and culture plates in the consumables storage area; a worktable is provided at the bottom of the work area, and a mechanical arm and a suction head 23 are provided above the worktable, and the mechanical arm passes through The lifting device is connected with the guide rail 21, the guide rail 21 can make the mechanical arm move back and forth, left and right, the lifting device can make the mechanical arm move up and down, and the head of the mechanical arm can rotate; The head 23 moves back and forth, left and right, and the lifting device can move the suction head 23 up and down. A specimen rack 17 is arranged on one side of the workbench, and a test tube is arranged in the specimen rack 17. The specimen collection swab body card 43 is arranged on the top of the test tube, and the specimen collection swab The body card 43 is matched with the sample holder recessed card 49 arranged on the top of the sample holder 17, and the test tube is also provided with a sample collection swab head card 41, and the bottom of the sample collection swab head card 41 is connected to the sample collection swab bar, and the workbench is in addition One side is provided with a culture bottle placement place 13 and a paraffin oil bottle placement place 31, and a camera 4 is arranged in the result interpretation area 7.
实施例二：参见图5、图6和图16Embodiment 2: See Figure 5, Figure 6 and Figure 16
培养瓶盖52和培养瓶底55均为多边形，石蜡油瓶盖70和石蜡油瓶底72均为多边形，设为多边形可以使得机械臂抓取培养瓶和石蜡油瓶更方便。The culture bottle cap 52 and the bottom 55 of the culture bottle are polygonal, and the paraffin oil bottle cap 70 and the bottom 72 of the paraffin oil bottle are polygonal. It is more convenient to make the manipulator grab the culture bottle and the paraffin oil bottle as a polygon.
实施例三：参见图5和图6Embodiment Three: See Figure 5 and Figure 6
培养瓶分男性标本用培养瓶和女性标本用培养瓶，男性标本用培养瓶上设有男性标本用培养瓶标本取刮器35，女性标本用培养瓶上设有女性标本用培养瓶标本取刮器54，男性标本采集拭子48在男性标本用培养瓶标本取刮器35内上下摩擦并旋转，以便将标本充分溶于培养液中，女性标本采集拭子46在女性标本用培养瓶标本取刮器54内上下摩擦并旋转，以便将标本充分溶于培养液中。The culture bottle is divided into a culture bottle for a male specimen and a culture bottle for a female specimen. The culture bottle for a male specimen is provided with a scraper 35 for a culture bottle for a male specimen, and the culture bottle for a female specimen is provided with a scraper 35 for a culture bottle for a female specimen. Device 54, male specimen collection swab 48 rubs and rotates up and down in the scraper 35 for male specimens, so that the specimen is fully dissolved in the culture solution, and female specimen collection swab 46 is used for female specimens. Rub and rotate up and down in the scraper 54, so that the specimen is fully dissolved in the culture solution.
实施例四：参见图1、图2、图3、图4、图5、图6、图7和图8Embodiment Four: Refer to Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7 and Fig. 8
本发明具体工作原理如下：Concrete working principle of the present invention is as follows:
操作前，检查吸头盒27中吸头、无菌吸头盒26中无菌吸头、培养板和培养箱的培养仓11和石蜡油瓶放置处31中培养瓶、培养板和石蜡油等耗材是否足够，确保够用，检查储存室温度调节和显示器33和培养箱温度调节和显示器34是否正常，将贴有条形码的标本依次插在标本架17上，男性用男性标本采集拭子采集标本，女性用女性标本采集拭子采集标本，将标本采集拭子体卡43卡在标本架凹卡49内备用，条码扫描器29读取条码信息，第二机械臂24从培养瓶储存室30抓取对应标本数量的培养瓶置于操作台15培养瓶放置处13备用，第二机械臂24从培养板储存室25抓取对应标本数量的培养板置于操作台15培养板放置处28备用，抓取培养瓶和培养板时，条码扫描器29读取培养瓶条码53和培养板条码58，通过条码指定并记录标本与对应的培养瓶和培养板，确保信息和结果正确传输，第一机械臂8打开培养板盖板56并翻转置于操作台15，第二机械臂24拧开培养瓶盖52并放在翻转的培养板盖板56上，培养瓶盖52和培养瓶底55均为非圆形多边形，便于第二机械臂24拧开培养瓶盖52，无菌Tip头吸取50微升上述无菌培养液于培养板阴性质控孔内，条码扫描器29读取标本采集拭子条码44信息，第二机械臂24通过滑杆51调整高度和位置，将标本抓手凹卡50卡住标本采集拭子头卡41，标本抓手24往上抓标本采集拭子头与试管分离，从标本架17上抓取男性标本采集拭子48（女性标本采集拭子46）在其对应的培养瓶中男性标本用培养瓶标本取刮器35（女性标本用培养瓶标本取刮器）内上下摩擦并旋转以将标本充分溶于培养液中，再将标本插回对应的试管内，吸头23将混有标本的培养液加入培养板剩下的培养孔中，各培养孔均50微升，将吸头打入废物盒12内，第二机械臂24拧紧培养瓶盖52并将培养瓶放入培养瓶培养箱16中进行培养。第二机械臂24将石蜡油瓶盖打开，石蜡油瓶盖70和石蜡油瓶底72均为非圆形多边形，便于第二机械臂24拧开石蜡油瓶盖70，吸头23从石蜡油瓶中吸取足量石蜡油依次加入50微升于培养板各培养孔中，第一机械臂8将培养板盖板盖56好并将培养板送入相应的培养仓中，培养板碰一下培养箱门61，培养箱门61自动朝内开，培养板被送入培养仓中，若干秒后，门自动关闭，进行培养并计时，24小时后，第一机械臂8碰一下培养仓门并自动弹开，第一机械臂8将培养24小时的培养板抓出并置于结果判读区7进行结果判读并由摄像头4拍照存档，再将培养板送入培养仓继续培养，培养48小时后，第一机械臂8将培养板抓出并结果判读区7进行结果判读并由摄像头4拍照存档，结合24小时和48小时结果将最终结果经信号线路3传入LIS系统待审，将培养结束并发送结果的培养板和培养瓶放入废物盒12内，结束一批标本的培养。Before operation, check the tips in the tip box 27, the sterile tips in the sterile tip box 26, the culture chamber 11 of the culture plate and the incubator, and the culture bottle, culture plate and paraffin oil in the place 31 where the paraffin oil bottle is placed. Whether the consumables are sufficient, ensure that they are sufficient, check whether the temperature adjustment and display 33 of the storage room and the temperature adjustment and display 34 of the incubator are normal, insert the specimens with barcodes on the specimen rack 17 in sequence, and collect specimens with male specimen collection swabs for males , women use a female specimen collection swab to collect samples, and the sample collection swab body card 43 is stuck in the concave card 49 of the sample rack for standby, the barcode scanner 29 reads the barcode information, and the second mechanical arm 24 grabs the sample from the culture bottle storage room 30 Take the culture bottles corresponding to the number of specimens and place them in the culture bottle place 13 of the operating table 15 for standby use, and the second mechanical arm 24 grabs the culture plates corresponding to the number of specimens from the culture plate storage room 25 and place them in the culture plate place 28 of the operation table 15 for standby use. When grabbing the culture bottle and culture plate, the barcode scanner 29 reads the culture bottle barcode 53 and the culture plate barcode 58, specifies and records the specimen and the corresponding culture bottle and culture plate through the barcode, to ensure the correct transmission of information and results, the first machine The arm 8 opens the culture plate cover 56 and turns it over and places it on the console 15. The second mechanical arm 24 unscrews the culture bottle cap 52 and places it on the turned culture plate cover 56. The culture bottle cap 52 and the culture bottle bottom 55 are both The non-circular polygon is convenient for the second mechanical arm 24 to unscrew the culture bottle cap 52, and the sterile Tip sucks 50 microliters of the above-mentioned sterile culture solution into the negative quality control hole of the culture plate, and the barcode scanner 29 reads the sample collection swab Barcode 44 information, the second mechanical arm 24 adjusts the height and position through the slide bar 51, and the specimen gripper recessed card 50 is stuck on the specimen collection swab head card 41, and the specimen gripper 24 is upwardly grasped and the specimen collection swab head is separated from the test tube , grab the male specimen collection swab 48 (female specimen collection swab 46) from the specimen holder 17, and put the culture bottle specimen scraper 35 for the male specimen in its corresponding culture bottle (the culture bottle specimen scraper for the female specimen) Rub up and down inside and rotate to fully dissolve the specimen in the culture solution, then insert the specimen back into the corresponding test tube, add the culture solution mixed with the specimen into the remaining culture wells of the culture plate with the suction head 23, each culture well is 50 Microliter, put the suction tip into the waste box 12, the second mechanical arm 24 tightens the culture bottle cap 52 and puts the culture bottle into the culture bottle incubator 16 for cultivation. The second mechanical arm 24 opens the paraffin oil bottle cap, and the paraffin oil bottle cap 70 and the bottom 72 of the paraffin oil bottle are non-circular polygons, which is convenient for the second mechanical arm 24 to unscrew the paraffin oil bottle cap 70, and the suction head 23 starts from the paraffin oil. Absorb enough paraffin oil from the bottle and add 50 microliters to each culture well of the culture plate in turn. The first mechanical arm 8 covers the culture plate cover 56 and sends the culture plate into the corresponding culture chamber. The culture plate is touched for cultivation. The chamber door 61 and the incubator door 61 are automatically opened inwards, and the culture plate is sent into the culture chamber. After a few seconds, the door is automatically closed, and the cultivation and timing are carried out. After 24 hours, the first mechanical arm 8 touches the culture chamber door and It pops up automatically, and the first robotic arm 8 grabs the culture plate cultured for 24 hours and places it in the result interpretation area 7 for result interpretation, and the camera 4 takes photos for archiving, and then sends the culture plate into the culture chamber to continue the culture. After 48 hours of culture , the first robotic arm 8 grabs the culture plate and interprets the results in the result interpretation area 7, and takes photos and archives them with the camera 4, and combines the 24-hour and 48-hour results to send the final results to the LIS system through the signal line 3 for review, and the culture is completed And the culture plate and the culture bottle that send result are put into waste box 12, finish the culture of a batch of specimen.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910398378.4ACN110093250A (en) | 2019-05-14 | 2019-05-14 | Full-automatic mycoplasma culture sample adding detector and culture sample adding detection method thereof |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910398378.4ACN110093250A (en) | 2019-05-14 | 2019-05-14 | Full-automatic mycoplasma culture sample adding detector and culture sample adding detection method thereof |
| Publication Number | Publication Date |
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| CN110093250Atrue CN110093250A (en) | 2019-08-06 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910398378.4APendingCN110093250A (en) | 2019-05-14 | 2019-05-14 | Full-automatic mycoplasma culture sample adding detector and culture sample adding detection method thereof |
| Country | Link |
|---|---|
| CN (1) | CN110093250A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022007452A1 (en)* | 2020-07-10 | 2022-01-13 | Tci Co., Ltd. | Devices and methods for preparing a specimen |
| CN115243841A (en)* | 2020-03-23 | 2022-10-25 | 株式会社派什纳诺赛康斯 | Medical swab storage method and medical swab storage device |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569546A (en)* | 2008-04-28 | 2009-11-04 | 赖鸿政 | Sample collector |
| CN103370616A (en)* | 2010-10-29 | 2013-10-23 | 恩姆菲舍尔科技公司 | Automated system for sample preparation and analysis |
| US20140377843A1 (en)* | 2013-06-23 | 2014-12-25 | King Abdulaziz City For Science And Technology | Automated microbiology laboratory instrument and system uses thereof |
| CN205398606U (en)* | 2016-03-07 | 2016-07-27 | 吉林大学 | Special incubator of multi -functional identification of bacteria system |
| CN105838582A (en)* | 2016-05-24 | 2016-08-10 | 江门市凯林贸易有限公司 | Automatic sample separating component for mycoplasma identification and drug sensitivity analysis |
| CN105866115A (en)* | 2016-05-24 | 2016-08-17 | 江门市凯林贸易有限公司 | Mycoplasma identification and drug sensitivity analysis system and using method thereof |
| CN105997156A (en)* | 2016-06-12 | 2016-10-12 | 杭州昕龙医疗科技有限公司 | Sampling swab for full-automatic pretreatment |
| CN106244455A (en)* | 2016-08-29 | 2016-12-21 | 宁波键生物科技有限公司 | Fully-automatic intelligent cell culture system and control method thereof |
| CN107267374A (en)* | 2017-08-01 | 2017-10-20 | 长春杰希医药科技有限公司 | Culture apparatus is transported in a kind of clinical sample collection |
| CN108537768A (en)* | 2017-03-02 | 2018-09-14 | 林雪峰 | A kind of test result automatic identification system of the pathogen culture based on camera |
| CN109055217A (en)* | 2018-09-21 | 2018-12-21 | 中国科学院苏州生物医学工程技术研究所 | high-throughput three-dimensional cell culture system |
| CN109201133A (en)* | 2018-11-13 | 2019-01-15 | 望潮科技（北京）有限公司 | A kind of mechanical arm experimental system |
| CN109342140A (en)* | 2018-10-30 | 2019-02-15 | 济南百博生物技术股份有限公司 | A kind of high-effective microorganism system for pretreating sample |
| CN109439545A (en)* | 2018-11-07 | 2019-03-08 | 深圳大学 | Biomedicine experiment equipment |
| CN208648690U (en)* | 2018-07-09 | 2019-03-26 | 薛敏强 | A kind of efficient, facilitation bottle cap installation mechanical arm |
| CN210683746U (en)* | 2019-05-14 | 2020-06-05 | 滕文友 | Full-automatic mycoplasma culture sample adding detector |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569546A (en)* | 2008-04-28 | 2009-11-04 | 赖鸿政 | Sample collector |
| CN103370616A (en)* | 2010-10-29 | 2013-10-23 | 恩姆菲舍尔科技公司 | Automated system for sample preparation and analysis |
| US20140377843A1 (en)* | 2013-06-23 | 2014-12-25 | King Abdulaziz City For Science And Technology | Automated microbiology laboratory instrument and system uses thereof |
| CN205398606U (en)* | 2016-03-07 | 2016-07-27 | 吉林大学 | Special incubator of multi -functional identification of bacteria system |
| CN105838582A (en)* | 2016-05-24 | 2016-08-10 | 江门市凯林贸易有限公司 | Automatic sample separating component for mycoplasma identification and drug sensitivity analysis |
| CN105866115A (en)* | 2016-05-24 | 2016-08-17 | 江门市凯林贸易有限公司 | Mycoplasma identification and drug sensitivity analysis system and using method thereof |
| CN105997156A (en)* | 2016-06-12 | 2016-10-12 | 杭州昕龙医疗科技有限公司 | Sampling swab for full-automatic pretreatment |
| CN106244455A (en)* | 2016-08-29 | 2016-12-21 | 宁波键生物科技有限公司 | Fully-automatic intelligent cell culture system and control method thereof |
| CN108537768A (en)* | 2017-03-02 | 2018-09-14 | 林雪峰 | A kind of test result automatic identification system of the pathogen culture based on camera |
| CN107267374A (en)* | 2017-08-01 | 2017-10-20 | 长春杰希医药科技有限公司 | Culture apparatus is transported in a kind of clinical sample collection |
| CN208648690U (en)* | 2018-07-09 | 2019-03-26 | 薛敏强 | A kind of efficient, facilitation bottle cap installation mechanical arm |
| CN109055217A (en)* | 2018-09-21 | 2018-12-21 | 中国科学院苏州生物医学工程技术研究所 | high-throughput three-dimensional cell culture system |
| CN109342140A (en)* | 2018-10-30 | 2019-02-15 | 济南百博生物技术股份有限公司 | A kind of high-effective microorganism system for pretreating sample |
| CN109439545A (en)* | 2018-11-07 | 2019-03-08 | 深圳大学 | Biomedicine experiment equipment |
| CN109201133A (en)* | 2018-11-13 | 2019-01-15 | 望潮科技（北京）有限公司 | A kind of mechanical arm experimental system |
| CN210683746U (en)* | 2019-05-14 | 2020-06-05 | 滕文友 | Full-automatic mycoplasma culture sample adding detector |
| Title |
|---|
| 张秀明等: "《临床微生物检验质量管理与标准操作程序》", 上海科学技术出版社, pages: 264* |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115243841A (en)* | 2020-03-23 | 2022-10-25 | 株式会社派什纳诺赛康斯 | Medical swab storage method and medical swab storage device |
| US20230083943A1 (en)* | 2020-03-23 | 2023-03-16 | Pipesh Nano Seconds Inc. | Medical swab storage method and medical swab storage device |
| WO2022007452A1 (en)* | 2020-07-10 | 2022-01-13 | Tci Co., Ltd. | Devices and methods for preparing a specimen |
| Publication | Publication Date | Title |
|---|---|---|
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|---|---|---|---|
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| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | Application publication date:20190806 | |
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