A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochlorideTechnical field
The present invention relates to micro-fluidic chip detection technique field, more particularly to a kind of three joint inspection micrometer of clenbuterol hydrochlorideFluidic chip.
Background technique
Micro-fluidic chip is the emerging technology that chemistry or biological sample are operated and detected on chip.It is micro-Fluidic chip can be integrated into the operating units such as the preparation of sample, reaction, separation and detection one several square centimeters of chipOn, by the manipulation and control to fluid in microchannel network, it is automatically performed analytic process.Since microfluidic chip technology hasSample volume is small, integrated level is high, Yi Shixian automation control and the characteristics of high throughput analysis, so that being given birth on micro-fluidic chipIt is more convenient, quick, low in cost to change the more conventional analysis sample pre-treatments of operation.
Clenbuterol hydrochloride is a kind of drug, rather than a kind of specific substance, is the feed addition for referring to promote lean meat growthAgent.It is any to promote lean meat growth, inhibit the substance of fat meat growth that can be called " clenbuterol hydrochloride ".It is a kind of adrenal gland classCNS stimulant.Currently, the substance that can be realized this function is the drug that one kind is called beta-stimulants (β-agonist), thanSuch as China cause poisoning Clenbuterol (clenbuterol) and the U.S. allow using Ractopamine/translate Lei Tuoba againAmine (Ractopamine).In China, usually said " clenbuterol hydrochloride " then refers to clenobuterol hydrochloride.It was once used as drug to useIn treatment bronchial asthma, after since its side effect is too big be banned use.There are also salbutamols for other such similar medicines(Salbutamol) and Terbutaline (Terbutaline) etc., " lean meat " can equally be played the role of, but to human health damage mistakeGreatly, thus security risk is caused.They are also thus disabled in the whole world.The time that its dosage is big, uses is long, metabolism is slow, instituteIt is all very big in the intracorporal residual quantity of pig to arrive listing before butchering.This residual quantity enters human body by food, just makes human body graduallyGradually it is poisoned, savings poisoning.If one time intake is excessive, will generate abnormal physiology reaction intoxicating phenomenon, it is serious canLead to death；Chromatograph test strip is a kind of method of rapid field detection clenbuterol hydrochloride at present.
Chromatograph test strip has been widely used in the fields such as clinical diagnosis, food safety, environment measuring.Detection method packetColoration, fluorescence, Magnetic testi etc. are included, depending on the marker in bonding pad, and if quantitative detection, such as fluorescence is needed to be measuredCleaning solution is added dropwise in liquid after reaction, and removal detection zone is not associated with marker, reduces interference.Operator is needed in routine operationBe added prepare liquid reaction wait for a period of time after, be manually added cleaning solution, then again with visually or easy detection device intoRow result is read, and the degree of automation is poor, influences detection efficiency.
Therefore, how a kind of clean hygiene, detection three joint inspection micrometer fluidic chip of clenbuterol hydrochloride accurate, easy to operate are providedThe problem of being those skilled in the art's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides a kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, by micro-fluidic chip and chromatographyTest strips combine, and meet multi objective while detecting, and liquid portion in the chip mostly, more much cleaner than independent test strips after reaction；LiquidBody enters next stage by centrifugation, and guarantees that different time enters different liquids by different centrifugal rotational speeds, and user is addingAfter prepare liquid, cleaning solution, transfers to centrifugation detecting instrument to complete follow-up work, without the waiting time and carry out subsequent manual behaviourMake, improves the degree of automation.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, comprising: the cover layer that sets gradually from top to bottom, first passageLayer, second channel layer and backplane level；Wherein, the cover layer, the first passage layer, the second channel layer and the bottom plateCooperation is sealed between layer；
The cover layer be provided with visual window, cover layer ventilation mouth, cover layer prepare liquid sample holes, cover layer cleaning solution intoSample hole, cover layer prepare liquid venthole, cover layer cleaning solution venthole, cover layer sample inlet pool venthole, cover layer cleaning solution masterVenthole in channel, venthole, cover board chip fixation hole in cover layer prepare liquid main channel；
The first passage layer includes detection zone through-hole I, detection zone through-hole II, first passage layer prepare liquid separating pool,One channel layer cleaning solution separating pool, first passage layer prepare liquid feed pathway, cleaning solution main channel, the ventilation of cleaning solution main channelHole, prepare liquid main channel, prepare liquid main channel venthole, first passage layer sample inlet pool, sample inlet pool venting channels, first passage layerSample inlet pool venthole, first passage layer prepare liquid sample holes, first passage layer cleaning solution sample holes, first passage layer cleaning solution masterVenthole in channel, venthole, buffer pool I, buffer pool II, first passage layer chip are solid in first passage layer prepare liquid main channelDetermine hole；
The detection zone through-hole I is corresponding with the cover layer ventilation mouth and is connected to；The detection zone through-hole II with it is described canForm is correspondingly arranged；
The first passage layer cleaning solution sample holes are corresponding with the cover layer cleaning solution sample holes and are connected to, the cleaningLiquid main channel is connected to the first passage layer cleaning solution sample holes and cleaning solution main channel venthole, and the cleaning solution master is logicalSeveral first passage layer cleaning solution separating pools, and two first passage layer cleaning solution separating pools are communicated on roadBetween the cleaning solution main channel on be provided with venthole in the first passage layer cleaning solution main channel；First passage layer is clearVenthole is correspondingly arranged with venthole in cover layer cleaning solution main channel in washing lotion main channel；The cleaning solution main channel is logicalStomata is corresponding with the cover layer cleaning solution venthole and is connected to；
The first passage layer prepare liquid sample holes are corresponding with the cover layer prepare liquid sample holes and are connected to, described to be measuredLiquid main channel is connected to the first passage layer prepare liquid sample holes and prepare liquid main channel venthole, and the prepare liquid master is logicalSeveral first passage layer prepare liquid separating pools, and two first passage layer prepare liquid separating pools are communicated on roadBetween the prepare liquid main channel on be provided with venthole in the first passage layer prepare liquid main channel；First passage layer toVenthole in liquid main channel is surveyed to be correspondingly arranged with venthole in cover layer prepare liquid main channel；The prepare liquid main channel is logicalStomata is corresponding with the cover layer prepare liquid venthole and is connected to；
The first passage layer prepare liquid feed pathway is connected to the first passage layer prepare liquid separating pool and described firstChannel layer sample inlet pool, and the buffer pool I is communicated in the first passage layer prepare liquid feed pathway；
The sample inlet pool venting channels are connected to the first passage layer sample inlet pool venthole and the first passage layer sample introductionPond, and the buffer pool II is communicated on the sample inlet pool venting channels；The first passage layer sample inlet pool venthole and instituteCover layer sample inlet pool venthole is stated to be correspondingly arranged；
The second channel layer includes detection zone through-hole III, detection zone through-hole IV, second channel layer prepare liquid separating pool,Two channel layer cleaning solution separating pools, second channel layer cleaning solution feed pathway, second channel layer sample inlet pool, second channel layer chipFixation hole；
The detection zone through-hole III is correspondingly arranged with the detection zone through-hole I；The detection zone through-hole IV and the detectionArea's through-hole II is correspondingly arranged；
The second channel layer prepare liquid separating pool is corresponding with first passage layer prepare liquid separating pool and is connected to；Described secondChannel layer cleaning solution separating pool is corresponding with the first passage layer cleaning solution separating pool and is connected to；Second channel layer cleaning solution feed liquorChannel is connected to the second channel layer sample inlet pool and the second channel layer cleaning solution separating pool；
The backplane level is provided with chromatograph test strip and bottom plate chip fixation hole, wherein the chromatograph test strip is by samplePad, nitrocellulose filter, bonding pad and absorption pad composition, wherein the nitrocellulose filter be located at centre, the sample pad andThe absorption pad is located at both ends；The bonding pad is between the sample pad and the nitrocellulose filter；
The chromatograph test strip has been arranged radially several, and each chromatograph test strip matches setting respectivelyThere are the corresponding second channel layer sample inlet pool, second channel layer prepare liquid separating pool, second channel layer cleaning solution separating pool, theTwo channel layer cleaning solution feed pathways, first passage layer prepare liquid separating pool, first passage layer cleaning solution separating pool, first passageLayer prepare liquid feed pathway, first passage layer sample inlet pool, sample inlet pool venting channels, first passage layer sample inlet pool venthole, bufferingPond I, buffer pool II, the detection zone through-hole I, the detection zone through-hole II, the detection zone through-hole III and the detection zone are logicalHole IV, the visual window, the cover layer ventilation mouth；
The sample pad of the chromatograph test strip is correspondingly arranged with the second channel layer sample inlet pool, and described secondChannel layer sample inlet pool is connected to the backplane level, and liquid can be contacted with the sample pad in the second channel layer sample inlet pool,So that liquid in the second channel layer sample inlet pool is flowed in the sample pad, the combination is stepped by chromatography effectPad, the nitrocellulose filter, the absorption pad carry out series reaction；The nitrocellulose of the chromatograph test stripFilm is correspondingly arranged with the detection zone through-hole IV, the detection zone through-hole II, the visual window, by the visual window and describedThe chromatograph test strip reactiveness can be observed in detection zone through-hole IV, the detection zone through-hole II；The absorption pad with it is describedDetection zone through-hole III, the detection zone through-hole I, cover layer ventilation mouth are correspondingly arranged, and gas flowing can occur, balance the layerThe air pressure in space where analysing test strips facilitates chromatography effect.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, the visual window is open type through-hole；InstituteStating to stick on the outer surface of venthole in venthole and cover layer prepare liquid main channel in cover layer cleaning solution main channel hasIt is tearable to take sealer；Blocked in liquid feeding with sealer or other objects, remove sealer after adding, by chip be put into necessary instrument fromThe heart leads in exposed cover layer cleaning solution main channel in venthole and cover layer prepare liquid main channel in centrifugal processStomata can guarantee that centrifugation sample introduction effect is more preferable.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, the second channel layer cleaning solution feed liquorThe shape in channel includes but are not limited to waveform, and waveform channel can increase passage length, can also be fold-line-shaped or sawTooth form.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, the cover board chip fixation hole with it is describedThe fixed hole shape of first passage layer chip fixation hole, the second channel layer chip fixation hole, the bottom plate chip is identical and againSetting is closed, is formed and runs through a kind of chip fixation hole of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride.Described in centrifugal processA kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride is fixed on centrifugation detector by the chip fixation hole, a kind of lean meatSmart three joint inspection micrometer fluidic chips are centrifuged by center of circle rotation of the chip fixation hole.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, the first passage layer cleaning solution liquid separationPond, the second channel layer cleaning solution separating pool, the first passage layer prepare liquid separating pool, the second channel layer prepare liquidSeparating pool is respectively positioned on the second channel layer sample inlet pool close to the side of the chip fixation hole.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, the cleaning solution main channel, described secondChannel layer cleaning solution separating pool, the prepare liquid main channel, the second channel layer prepare liquid separating pool, the second channel layerSample inlet pool is by closely to being far successively arranged in chip fixation hole side.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, a kind of three joint inspection micrometer of clenbuterol hydrochlorideThe driving force of liquid is centrifugal force in fluidic chip.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, second channel layer prepare liquid separating pool,Two channel layer cleaning solution separating pools, second channel layer cleaning solution feed pathway and the backplane level completely cut off.
Preferably, the cleaning solution main channel, described to be measured in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochlorideLiquid main channel, the first passage layer prepare liquid feed pathway, the sample inlet pool venting channels are to be built in the first passagePipeline in layer；The second channel layer cleaning solution feed pathway is the pipeline being built in the second channel layer.
Preferably, in a kind of above-mentioned three joint inspection micrometer fluidic chip of clenbuterol hydrochloride, the chromatograph test strip is radial to be setThere are three setting, respectively clenbuterol hydrochloride clenobuterol hydrochloride Test paper, Ractopamine Test paper and salbutamol detection examinationPaper.
When liquid feeding, venthole and the cover layer prepare liquid master in cover layer cleaning solution main channel are blocked using sealerVenthole in channel passes through cover layer prepare liquid sample holes, cover layer cleaning solution sample holes, first passage layer prepare liquid sample introductionHole, first passage layer cleaning solution sample holes add to prepare liquid and cleaning solution in prepare liquid main channel and cleaning solution main channel, andIt is exhausted by prepare liquid main channel venthole and cleaning solution main channel venthole；Prepare liquid passes through prepare liquid primary passageway stream extremelyIn the semiclosed chamber that first passage layer prepare liquid separating pool and second channel layer prepare liquid separating pool are formed, and it is full of one by one；Cleaning solution is formed by cleaning solution primary passageway stream to first passage layer cleaning solution separating pool and second channel layer cleaning solution separating poolSemiclosed chamber in, and be full of one by one；After cleaning solution and prepare liquid fill, remove sealer.
When buffer pool I guarantees to add prepare liquid, multiple first passage layer prepare liquid separating pools and second channel layer prepare liquid pointIt is each filled with liquid in the semiclosed chamber that liquid pool is formed, and liquid does not enter first passage layer sample inlet pool and second channel layer sample introductionIn the semiclosed chamber that pond is formed.
Buffer pool II guarantees the semiclosed chamber that first passage layer sample inlet pool and second channel layer sample inlet pool are formed when centrifugationMiddle liquid will not be thrown away by sample inlet pool venting channels and be polluted outside chip.
It can be seen via above technical scheme that compared with prior art, the present disclosure provides a kind of clenbuterol hydrochlorides threeDetection micro-fluidic chip meets multi objective while detecting by micro-fluidic chip in conjunction with chromatograph test strip, improves working efficiency,Liquid portion in the chip mostly, more much cleaner than independent test strips after reaction；Liquid enters next stage by centrifugation, and passes through differenceCentrifugal rotational speed guarantee that different time enters different liquids, user after adding prepare liquid, cleaning solution, transfer to centrifugation detecting instrumentFollow-up work is completed, without the waiting time and carries out subsequent manual operation, improves the degree of automation.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show belowThere is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only thisThe embodiment of invention for those of ordinary skill in the art without creative efforts, can also basisThe attached drawing of offer obtains other attached drawings.
Fig. 1 attached drawing is three-dimensional explosive view of the invention；
Fig. 2 attached drawing is cover layer structure chart；
Fig. 3 attached drawing is first passage layer structure chart；
Fig. 4 attached drawing is second channel layer structure chart；
Fig. 5 attached drawing is bottom plate schematic diagram of a layer structure；
Fig. 6 attached drawing is chromatograph test strip structural schematic diagram.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, completeSite preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based onEmbodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every otherEmbodiment shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a kind of three joint inspection micrometer fluidic chips of clenbuterol hydrochloride, by micro-fluidic chip and chromatographic test paperItem combines, and meets multi objective while detecting, raising working efficiency, liquid portion in the chip mostly after reaction, than independent test stripsIt is much cleaner；Liquid enters next stage by centrifugation, and guarantees that different time enters different liquids by different centrifugal rotational speeds, usesCentrifugation detecting instrument is transferred to complete follow-up work, after waiting time and progress after adding prepare liquid, cleaning solution in familyContinuous manual operation, improves the degree of automation.
In conjunction with attached drawing 1-6, the invention discloses a kind of three joint inspection micrometer fluidic chips of clenbuterol hydrochloride, comprising: from top to bottom according toCover layer 1, first passage layer 2, second channel layer 3 and the backplane level 4 of secondary setting；Wherein, cover layer 1, first passage layer 2,Cooperation is sealed between two channel layers 3 and backplane level 4；
Cover layer 1 is provided with visual window 11, cover layer ventilation mouth 12, cover layer prepare liquid sample holes 13, cover layer cleaningLiquid sample holes 14, cover layer prepare liquid venthole 15, cover layer cleaning solution venthole 16, cover layer sample inlet pool venthole 17, lidVenthole 18 in plate layer cleaning solution main channel, venthole 19, cover board chip fixation hole 110 in cover layer prepare liquid main channel；
First passage layer 2 include detection zone through-hole I 21, detection zone through-hole II 22, first passage layer prepare liquid separating pool 23,First passage layer cleaning solution separating pool 24, first passage layer prepare liquid feed pathway 25, cleaning solution main channel 26, cleaning solution master are logicalRoad venthole 27, prepare liquid main channel 28, prepare liquid main channel venthole 29, first passage layer sample inlet pool 210, sample inlet pool ventilationChannel 211, first passage layer sample inlet pool venthole 212, first passage layer prepare liquid sample holes 213, first passage layer cleaning solutionVenthole 215 in sample holes 214, first passage layer cleaning solution main channel, venthole in first passage layer prepare liquid main channel216, buffer pool I 217, buffer pool II 218, first passage layer chip fixation hole 219；
Detection zone through-hole I 21 is corresponding with cover layer ventilation mouth 12 and is connected to；Detection zone through-hole II 22 is corresponding with visual window 11Setting；
First passage layer cleaning solution sample holes 214 are corresponding with cover layer cleaning solution sample holes 14 and are connected to, and cleaning solution master is logicalRoad 26 is connected to first passage layer cleaning solution sample holes 214 and cleaning solution main channel venthole 27, is connected on cleaning solution main channel 26There are several first passage layer cleaning solution separating pools 24, and the cleaning solution between two first passage layer cleaning solution separating pools 24Venthole 215 in first passage layer cleaning solution main channel is provided on main channel 26；Lead in first passage layer cleaning solution main channelStomata 215 is correspondingly arranged with venthole 18 in cover layer cleaning solution main channel；Cleaning solution main channel venthole 27 is clear with cover layerWashing lotion venthole 16 is corresponding and is connected to；
First passage layer prepare liquid sample holes 213 are corresponding with cover layer prepare liquid sample holes 13 and are connected to, and prepare liquid master is logicalRoad 28 is connected to first passage layer prepare liquid sample holes 213 and prepare liquid main channel venthole 29, is connected on prepare liquid main channel 28There are several first passage layer prepare liquid separating pools 23, and the prepare liquid between two first passage layer prepare liquid separating pools 23Venthole 216 in first passage layer prepare liquid main channel is provided on main channel 28；Lead in first passage layer prepare liquid main channelStomata 216 is correspondingly arranged with venthole 19 in cover layer prepare liquid main channel；Prepare liquid main channel venthole 29 is waited for cover layerLiquid venthole 15 is surveyed to correspond to and be connected to；
First passage layer prepare liquid feed pathway 25 be connected to first passage layer prepare liquid separating pool 23 and first passage layer intoSample pond 210, and buffer pool I 217 is communicated in first passage layer prepare liquid feed pathway 25；
Sample inlet pool venting channels 211 are connected to first passage layer sample inlet pool venthole 212 and first passage layer sample inlet pool 210,And buffer pool II 218 is communicated on sample inlet pool venting channels 211；First passage layer sample inlet pool venthole 212 and cover layer intoSample pond venthole 17 is correspondingly arranged；
Second channel layer 3 includes detection zone through-hole III 31, detection zone through-hole IV 32, second channel layer prepare liquid separating pool33, second channel layer cleaning solution separating pool 34, second channel layer cleaning solution feed pathway 35, second channel layer sample inlet pool 36,Two channel layer chip fixation holes 37；
Detection zone through-hole III 31 is correspondingly arranged with detection zone through-hole I 21；Detection zone through-hole IV 32 and detection zone through-hole II 22It is correspondingly arranged；
Second channel layer prepare liquid separating pool 33 is corresponding with first passage layer prepare liquid separating pool 23 and is connected to；Second channelLayer cleaning solution separating pool 34 is corresponding with first passage layer cleaning solution separating pool 24 and is connected to；Second channel layer cleaning solution feed pathway35 connection second channel layer sample inlet pool 36 and second channel layer cleaning solution separating pool 34；
Backplane level 4 is provided with chromatograph test strip 41 and bottom plate chip fixation hole 42, and wherein chromatograph test strip 41 is by sample pad43, nitrocellulose filter 44, bonding pad 45 and absorption pad 46 form, and wherein nitrocellulose filter 44 is located at centre, sample pad 43Both ends are located at absorption pad 46；Bonding pad 45 is between sample pad 43 and nitrocellulose filter 44；
Chromatograph test strip 41 has been arranged radially several, and each chromatograph test strip 41 matches be provided with phase respectivelySecond channel layer sample inlet pool 36, second channel layer prepare liquid separating pool 33, the second channel layer cleaning solution separating pool 34, second answeredChannel layer cleaning solution feed pathway 35, first passage layer prepare liquid separating pool 23, first passage layer cleaning solution separating pool 24, firstChannel layer prepare liquid feed pathway 25, first passage layer sample inlet pool 210, sample inlet pool venting channels 211, first passage layer sample inlet poolVenthole 212, buffer pool I 217, buffer pool II 218, detection zone through-hole I 21, detection zone through-hole II 22, detection zone through-hole III 31With detection zone through-hole IV 32, visual window 11, cover layer ventilation mouth 12；
The sample pad 43 of chromatograph test strip 41 is correspondingly arranged with second channel layer sample inlet pool 36, and second channel layer sample introductionPond 36 is connected to backplane level 4, and liquid can be contacted with sample pad 43 in second channel layer sample inlet pool 36, makes second channel layer sample introductionLiquid can flow in sample pad 43 in pond 36, step into bonding pad 45, nitrocellulose filter 44, absorption pad by chromatography effect46, carry out series reaction；The nitrocellulose filter 44 and detection zone through-hole IV 32, detection zone through-hole II of chromatograph test strip 4122, visual window 11 is correspondingly arranged, and chromatography examination can be observed by visual window 11 and detection zone through-hole IV 32, detection zone through-hole II 2241 reactiveness of paper slip；Absorption pad 46 is correspondingly arranged with detection zone through-hole III 31, detection zone through-hole I 21, cover layer ventilation mouth 12,Gas flowing can occur, balance the air pressure in 41 place space of chromatograph test strip, facilitate chromatography effect.
In order to further optimize the above technical scheme, visual window 11 is open type through-hole；In cover layer cleaning solution main channelStick to have on the outer surface of venthole 19 in venthole 18 and cover layer prepare liquid main channel and tearable takes sealer；It is sealed in liquid feedingFilm or other objects are blocked, and remove sealer after adding, and chip is put into necessary instrument and is centrifuged, exposed cover layer in centrifugal processIn cleaning solution main channel in venthole 18 and cover layer prepare liquid main channel venthole 19 can guarantee to be centrifuged sample introduction effect it is more preferable.
In order to further optimize the above technical scheme, the shape of second channel layer cleaning solution feed pathway 35 includes but not onlyIt is limited to waveform, waveform channel can increase passage length, can also be fold-line-shaped or zigzag.
In order to further optimize the above technical scheme, cover board chip fixation hole 110 and first passage layer chip fixation hole219, second channel layer chip fixation hole 37, bottom plate chip 42 shapes of fixation hole are identical and are overlapped setting, are formed through a kind of thinThe chip fixation hole of meat three joint inspection micrometer fluidic chips of essence.A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride is logical in centrifugal processIt crosses chip fixation hole to be fixed on centrifugation detector, a kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride is using chip fixation hole as the center of circleRotation is centrifuged.
In order to further optimize the above technical scheme, first passage layer cleaning solution separating pool 24, second channel layer cleaning solutionSeparating pool 34, first passage layer prepare liquid separating pool 23, second channel layer prepare liquid separating pool 33 be respectively positioned on second channel layer intoSample pond 36 is close to the side of chip fixation hole.
In order to further optimize the above technical scheme, cleaning solution main channel 26, second channel layer cleaning solution separating pool 34, toLiquid main channel 28, second channel layer prepare liquid separating pool 33, second channel layer sample inlet pool 36 are surveyed by closely to being far successively arranged in corePiece fixation hole side.
In order to further optimize the above technical scheme, in a kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride liquid driving forceFor centrifugal force.
In order to further optimize the above technical scheme, second channel layer prepare liquid separating pool 33, second channel layer cleaning solutionSeparating pool 34, second channel layer cleaning solution feed pathway 35 and backplane level 4 completely cut off.
In order to further optimize the above technical scheme, cleaning solution main channel 26, prepare liquid main channel 28, first passage layer toSurvey liquid feed pathway 25, sample inlet pool venting channels 211 are the pipeline being built in first passage layer 2；Second channel layer cleaning solutionFeed pathway 35 is the pipeline being built in second channel layer 3.
In order to further optimize the above technical scheme, there are three chromatograph test strip 41 is arranged radially, respectively lean meatRefined salt Clenbuterol Test paper, Ractopamine Test paper and salbutamol Test paper.
When liquid feeding, blocked in cover layer cleaning solution main channel in venthole 18 and cover layer prepare liquid main channel using sealerVenthole 19 passes through cover layer prepare liquid sample holes 13, cover layer cleaning solution sample holes 14, first passage layer prepare liquid sample holes213, prepare liquid and cleaning solution are added to prepare liquid main channel 28 and cleaning solution main channel by first passage layer cleaning solution sample holes 214In 26, and it is exhausted by prepare liquid main channel venthole 29 and cleaning solution main channel venthole 27；Prepare liquid passes through to be measuredLiquid main channel 28 flow to first passage layer prepare liquid separating pool 23 and second channel layer prepare liquid separating pool 33 formed it is semiclosedIn chamber, and it is full of one by one；Cleaning solution flow to first passage layer cleaning solution separating pool 24 and second by cleaning solution main channel 26In the semiclosed chamber that channel layer cleaning solution separating pool 34 is formed, and it is full of one by one；After cleaning solution and prepare liquid fill, goFall sealer.
When buffer pool I 217 guarantees to add prepare liquid, multiple first passage layer prepare liquid separating pools 23 and second channel layer are to be measuredIt is each filled with liquid in the semiclosed chamber that liquid separating pool 33 is formed, and liquid does not enter first passage layer sample inlet pool 210 and secondIn the semiclosed chamber that channel layer sample inlet pool 36 is formed.
Buffer pool II 218 guarantees first passage layer sample inlet pool 210 and second channel layer sample inlet pool 36 are formed when centrifugation halfLiquid will not be thrown away by sample inlet pool venting channels 211 and be polluted outside chip in closed chamber.
A kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride is fixed in the rotary shaft of centrifugation detector by chip fixation hole,It is centrifuged a kind of three joint inspection micrometer fluidic chip of clenbuterol hydrochloride with the rotation of the chip fixation hole center of circle, liquid flow process is such asUnder:
(1) slow-speed of revolution is centrifuged, and prepare liquid is from multiple first passage layer prepare liquid separating pools 23 and second channel layer prepare liquidEnter first passage through first passage layer prepare liquid feed pathway 25, buffer pool I 217 in the semiclosed chamber that separating pool 33 is formedIn the semiclosed chamber that layer sample inlet pool 210 and second channel layer sample inlet pool 36 are formed；
(2) after centrifugation detector low speed effect, static, first passage layer sample inlet pool 210 and second channel layer sample inlet poolThe sample pad 43 of prepare liquid contact chromatograph test strip 41 in the 36 semiclosed chambers formed, steps into bonding pad by chromatography effect45, nitrocellulose filter 44, absorption pad 46 carry out series reaction, and prepare liquid is reacted with marker in bonding pad 45, lead toCover layer ventilation mouth 12, detection zone through-hole I 21, detection zone through-hole III 31 is crossed to arrange the gas in 41 place space of chromatograph test stripOut, equilibrium air pressure；
(3) high speed centrifugation, cleaning solution is from first passage layer cleaning solution separating pool 24 and second channel layer cleaning solution separating poolEnter first passage layer sample inlet pool 210 and second through second channel layer cleaning solution feed pathway 35 in the 34 semiclosed chambers formedIn the semiclosed chamber that channel layer sample inlet pool 36 is formed；
(4) static after being centrifuged detector high velocity effect, first passage layer sample inlet pool 210 and second channel layer sample inlet poolThe sample pad 43 of the 36 semiclosed chamber cleaning liquid inside contact chromatograph test strips 41 formed enters bonding pad 45, nitre by chromatography effectRemnants on nitrocellulose filter 44 are not associated with reagent and removed by acid cellulose film 44, absorption pad 46, when result being avoided to readInterference, by cover layer ventilation mouth 12, detection zone through-hole I 21, detection zone through-hole III 31 by 41 place space of chromatograph test stripGas discharge, equilibrium air pressure.
(5) it is observed by detection zone through-hole II 22, detection zone through-hole IV 32, visual window 11 anti-on nitrocellulose filter 44Answer result.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with otherThe difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodimentFor, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is said referring to method partIt is bright.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined hereinGeneral Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the inventionIt is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase oneThe widest scope of cause.