Magnetic bead cleaning device suitable for in-vitro diagnostic equipmentTechnical Field
The invention relates to in-vitro diagnostic equipment, in particular to a magnetic bead cleaning device suitable for in-vitro diagnostic equipment.
Background
Existing in vitro diagnostic devices (such as chemiluminescent immunoassay analyzer, biochemical analyzer, nucleic acid analyzer, etc.) mostly use a chemiluminescent or bioluminescent system as an indicator system for antigen-antibody reaction, thereby quantitatively detecting antigens or antibodies. When separating and purifying a sample, magnetic beads are generally utilized to adsorb specific antibodies or receptors (i.e. target bodies) in the sample, then waste liquid in a reaction cup is sucked out, injected liquid is washed, and substances which are not combined with antigens or antibodies in the reaction liquid and other interference substances loaded on the magnetic beads are removed. From this, the bead washing is a critical factor affecting the detection result. The existing magnetic bead cleaning device consists of a hair washing component and a reaction cup rotating disc component, and waste liquid of the reaction cup is sucked or cleaning liquid is injected through the whole lifting of the hair washing component.
The invention patent CN103599898A discloses a full-automatic chemiluminescence immunoassay analyzer magnetic bead cleaning device, which comprises a lifting needle frame driven by a screw motor, wherein four liquid-discharging needles and four liquid-sucking needles are arranged on the lifting needle frame in a staggered manner, a rotary disk driven by a transmission mechanism is correspondingly arranged below the lifting needle frame, an inner sliding cylinder is arranged on a mounting plate below the rotary disk, the outer surface of the inner sliding cylinder is provided with magnets, namely, the magnets are all arranged in a reaction cup, the effective cleaning area of the magnetic beads is small, and the cleaning effect is poor; meanwhile, as the magnets are arranged at the liquid suction positions, the magnets are not arranged at the liquid injection positions, and the pre-condensation of the magnetic beads cannot be realized, so that the magnetic beads are lost; secondly, four liquid-spitting needles and four liquid-suction needles are arranged in a crossed mode, the liquid-suction needles are all single tubes, when the liquid-suction needles are used for sucking high-concentration high-viscosity sample waste liquid, the sample waste liquid is easy to attach to the needle wall of the liquid-suction needles, the outer wall of the liquid-suction needles is difficult to clean, cross contamination is caused between different samples, accuracy of detection results is affected, and repeatability is poor.
Disclosure of Invention
The invention aims to provide a magnetic bead cleaning device suitable for in-vitro diagnostic equipment, which not only can avoid cross contamination, but also can ensure the cleaning effect of magnetic beads, ensure that interfering substances adhered on the magnetic beads are completely cleaned, and also can avoid the loss of the magnetic beads.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the invention relates to a magnetic bead cleaning device suitable for in-vitro diagnostic equipment, which comprises a magnetic cleaning component arranged on a base plate, wherein a vertical plate at one side of the base plate is provided with a hair cleaning component driven by a lifting mechanism in a sliding manner, and the magnetic cleaning component and the hair cleaning component are correspondingly arranged up and down; a notch is formed in a mounting seat of the hair washing assembly, a liquid suction needle with a self-cleaning function is arranged at one edge of the notch, and a filling and sucking cleaning combination consisting of a liquid filling needle and a liquid suction needle is uniformly arranged on the mounting seat at intervals along the circumferential direction of the liquid suction needle; the magnetic washing assembly comprises a rotating disc driven to rotate by a power source, a plurality of mounting holes for containing reaction cups are uniformly arranged on the rotating disc at intervals along the circumferential direction of the rotating disc, a magnet seat is arranged below the rotating disc and corresponds to the mounting holes, the magnet seat comprises a connecting plate and a guide groove arranged at the outer edge of the connecting plate, pre-adsorption position magnets are arranged on the side wall of the guide groove and correspond to the notch, liquid pumping level magnets are arranged on the side wall of the guide groove and correspond to the liquid pumping point, magnet groups are arranged on the side wall of the guide groove and correspond to the one-injection one-suction cleaning combination, and each magnet group comprises liquid injection level magnets and liquid suction position magnets vertically corresponding to the liquid suction needles.
The mounting seat of the hair washing assembly is replaced by a linear structure, and the liquid suction needle and the one injection one suction cleaning combination are arranged at intervals along the length direction of the mounting seat; the rotary disk of the magnetic washing assembly is replaced by a bearing disk of a strip-shaped structure, and the guide groove is correspondingly replaced by a strip-shaped structure.
The liquid injection level magnets of the magnet group are arranged on the two side walls of the guide groove in pairs, and the liquid absorption level magnets of the magnet group are arranged on the two side walls of the guide groove in pairs.
The liquid injection level magnet of the magnet group is arranged on the inner side wall of the guide groove, and the liquid absorption level magnet of the magnet group is arranged on the outer side wall of the guide groove; or the liquid injection level magnet of the magnet group is arranged on the outer side wall of the guide groove, and the liquid absorption level magnet of the magnet group is arranged on the inner side wall of the guide groove.
The pre-adsorption position magnets are arranged on two side walls of the guide groove in pairs, and the pumping position magnets are arranged on two side walls of the guide groove in pairs.
The pre-adsorption position magnet is arranged on the inner side wall of the guide groove, and the pumping level magnet is arranged on the inner side wall of the guide groove; or the pre-adsorption position magnet and the pumping level magnet are arranged on the same side.
The liquid suction needle is a sleeve needle and comprises a T-shaped connecting seat formed by a fixed seat and a cross rod, an outer cleaning needle extending outwards is inserted in a long groove of the cross rod, an inner liquid suction needle is connected in the outer cleaning needle through a fixing component, the outer end of the inner liquid suction needle extends outwards to form an inserting section out of the outer end of the outer cleaning needle, the inner end of the inner liquid suction needle sequentially penetrates outwards to form an installing section through the outer cleaning needle and the fixed seat, and an inner pipeline joint is sleeved on the installing section; an outer cleaning channel communicated with the long groove is formed in the fixing seat located on one side of the inner liquid suction needle, and an outer pipeline connector communicated with the outer cleaning channel is arranged on the fixing seat.
The fixing assembly is a fixing groove formed by the concave of the needle wall of the outer cleaning needle, a fixing boss formed by the convex of the needle wall of the inner liquid suction needle, a positioning column clamped between the inner liquid suction needle and the outer cleaning needle, or a fixing ring sleeved on the inner liquid suction needle, and fixing blocks are arranged on the fixing ring at intervals.
The drainage needle is a needle, the needle comprises a needle tube fixedly connected to the mounting seat, a cleaning pool corresponding to the needle tube up and down is arranged above the magnetic cleaning assembly through a connecting piece, a pair of perforations matched with the needle tube are formed in the top wall and the bottom wall of the cleaning pool, a water inlet connector is arranged on the left side wall of the cleaning pool, and a water drainage connector is arranged on the right side wall of the cleaning pool.
The power source comprises a motor erected at the bottom of the substrate, the motor is connected with a vertical rotating shaft through a driving wheel in a transmission way, the vertical rotating shaft is rotatably arranged in a fixed sleeve on the substrate, and the rotating disc is fixedly connected to the top of the vertical rotating shaft through a bolt; the connecting plate is fixedly connected to the fixing sleeve.
The invention has the advantages that the liquid suction needle with the self-cleaning function is added on the hair washing component, the liquid suction needle is firstly utilized to suck out the sample waste liquid which does not participate in the reaction during the magnetic washing, the liquid suction needle is self-cleaned after the sample waste liquid is completely sucked out, the cleaning effect is good, and the cross contamination of different samples is completely avoided; a liquid injection level magnet is arranged on a guide groove corresponding to the liquid injection point, and the liquid suction level magnet and the liquid injection level magnet are crossed and opposite, or the liquid suction level magnet and the liquid injection level magnet are arranged in pairs, so that the cleaning area of the magnetic beads is maximized, and the cleaning effect is further ensured; meanwhile, the liquid injection level magnet has a pre-condensation function, so that the loss of the magnetic beads is minimized, and the probability of the loss of the magnetic beads is reduced.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Figure 2 is a schematic view of the structure of the hair washing assembly of the present invention.
Fig. 3 is a schematic cross-sectional view of the present invention.
Fig. 4 is a layout of a magnet assembly of the present invention.
Fig. 5 is a schematic view of the structure of the sleeve needle according to the present invention.
Fig. 6 is a schematic cross-sectional structure of fig. 5.
Fig. 7.1 is a schematic view of another construction of the fastening assembly according to the present invention.
Fig. 7.2 is a schematic view of a third construction of the fastening assembly according to the present invention.
Fig. 7.3 is a schematic view of a fourth construction of the fastening assembly according to the present invention.
Fig. 8 is a schematic view of another construction of the present invention.
Fig. 9 is a schematic view of the structure of the needle of fig. 8.
Detailed Description
As shown in fig. 1-3, the magnetic bead cleaning device suitable for in-vitro diagnostic equipment comprises a magnetic cleaning component arranged on a base plate 1, wherein a vertical plate 2 at one side of the base plate 1 is provided with a hair washing component driven by a lifting mechanism in a sliding manner, and the magnetic cleaning component and the hair washing component are correspondingly arranged up and down; a notch is formed in the mounting seat 3.1 of the hair washing assembly, a liquid suction needle with a self-cleaning function is arranged at one edge of the notch, five groups of one-injection one-suction cleaning combinations (of course, one group or two groups or three groups or four groups or more groups of one-injection one-suction cleaning combinations can be also arranged according to actual requirements) consisting of the liquid injection needle 3.2 and the liquid suction needle 3.3 are uniformly arranged on the mounting seat 3.1 at intervals along the circumferential direction of the liquid suction needle; the magnetic washing assembly comprises a rotating disk 4.1 driven to rotate by a power source, wherein the power source comprises a motor 7.1 erected at the bottom of the base plate 1, the motor 7.1 is connected with a vertical rotating shaft 7.2 through transmission of a transmission wheel, the vertical rotating shaft 7.2 is rotationally arranged in a fixed sleeve 7.3 on the base plate 1, the rotating disk 4.1 is fixedly connected at the top of the vertical rotating shaft 7.2 through bolts (of course, in actual manufacturing, an installation seat 3.1 of the head washing assembly can be made into a linear structure, a liquid suction needle is positioned at the end part of the installation seat 3.1, a one-injection one-suction cleaning combination is arranged along the length direction of the installation seat 3.1 from the liquid suction needle, and the intervals among the liquid suction needle, the liquid injection needle and the liquid suction needle are all equal to the intervals between two adjacent reaction cups;
as shown in fig. 3 and 4, a plurality of mounting holes 4.2 for accommodating reaction cups are uniformly arranged on the rotating disk 4.1 at intervals along the circumferential direction, a magnet seat is arranged below the rotating disk 4.1 corresponding to the plurality of mounting holes 4.2, the magnet seat comprises a connecting plate 4.3 and a guide groove 4.4 arranged at the outer edge of the connecting plate 4.3, and the connecting plate 4.3 is fixed on the fixed sleeve 7.3; the outer side wall of the guide groove 4.4 corresponding to the notch is provided with a pre-adsorption position magnet 4.5, the inner side wall of the guide groove 4.4 corresponding to the liquid suction point is provided with a liquid suction position magnet 4.6 (of course, when in actual installation, the pre-adsorption position magnet 4.5 and the liquid suction position magnet 4.6 are arranged on the inner side wall of the guide groove 4.4, of course, the pre-adsorption position magnets 4.5 can also be arranged on the two side walls of the guide groove 4.4 in pairs, the liquid suction position magnets 4.6 are arranged on the two side walls of the guide groove 4.4 in pairs), the distance between the pre-adsorption position magnets 4.5 and the liquid suction position magnets 4.6 is consistent with the hole distance between two adjacent mounting holes 4.2, and the guide groove 4.4 between the pre-adsorption position magnets 4.5 and the fifth group of magnets is a non-magnet area;
as shown in fig. 4, the side walls of the guide grooves 4.4 corresponding to the one-injection one-suction cleaning combination are provided with magnet groups, the guide grooves 4.4 corresponding to the liquid injection needles 3.2 are liquid injection levels, the guide grooves corresponding to the liquid suction needles are liquid suction levels, the magnet groups comprise liquid injection level magnets 4.7 arranged at the liquid injection levels and liquid suction level magnets 4.8 arranged at the liquid suction levels, and the first magnet group, the third magnet group and the fourth magnet group are all arranged in the following manner: the liquid injection level magnet 4.7 is arranged on the inner side wall of the guide groove 4.4, and the liquid absorption level magnet 4.8 of the magnet group is arranged on the outer side wall of the guide groove 4.4; as shown in fig. 4, the second group of magnets and the fourth group of magnets are arranged as follows: the liquid injection level magnets 4.7 of the magnet group are arranged on the outer side wall of the guide groove 4.4, the liquid absorption level magnets 4.8 of the magnet group are arranged on the inner side wall of the guide groove 4.4 (of course, according to the actual requirement, the magnet group can also be arranged in the following way, the liquid injection level magnets 4.7 of the magnet group are arranged on the two side walls of the guide groove 4.4 in pairs, and the liquid absorption level magnets 4.8 of the magnet group are arranged on the two side walls of the guide groove 4.4 in pairs), so that the cleaning area of the magnetic beads is maximized, the complete cleaning of the interfering substances on the magnetic beads is ensured, and the cleaning effect is good; the liquid injection level magnet 4.7 can ensure that the magnetic beads are condensed before imbibition, and the magnetic beads are prevented from being lost.
As shown in fig. 5 and 6, the liquid suction needle is a sleeve needle, and comprises a T-shaped connecting seat 5.1 formed by a fixed seat and a cross rod, an outer cleaning needle 5.3 extending outwards is inserted into a long groove 5.2 of the cross rod, an inner liquid suction needle 5.4 is connected in the outer cleaning needle 5.3 through a fixing component, the outer end of the inner liquid suction needle 5.4 extends outwards to form an insertion section at the outer end of the outer cleaning needle 5.3, the inner end of the inner liquid suction needle 5.4 sequentially penetrates out of the outer cleaning needle 5.3 and the fixed seat to form a mounting section, and an inner pipeline joint 5.5 is sleeved on the mounting section; an outer cleaning channel 5.6 communicated with the long groove 5.2 is arranged on the fixing seat at one side of the inner liquid suction needle 5.4, and an outer pipeline joint 5.7 communicated with the outer cleaning channel 5.6 is arranged on the fixing seat.
As shown in fig. 5, the fixing components are two fixing grooves 5.8 formed by concave inner walls of the outer cleaning needle 5.3; of course, the fixing component can also be two fixing bosses 5.9 formed by the outer convex of the needle wall of the inner liquid suction needle 5.4, as shown in fig. 7.1; two positioning posts 5.10 clamped between the inner liquid suction needle 5.4 and the outer cleaning needle 5.3 can also be used, and the positioning posts are particularly shown in fig. 7.2; the fixing ring 5.11 is sleeved on the inner liquid suction needle 5.4, and fixing blocks 5.12 are arranged on the fixing ring 5.11 at intervals, as shown in fig. 7.3;
when the sample waste liquid is extracted, the head washing assembly drives the sleeve needle to descend, the inserting section of the inner liquid suction needle 5.4 is inserted into the sample waste liquid in the reaction cup, the bottom of the outer cleaning needle 5.3 is located above the sample waste liquid, the liquid suction system sucks the sample into the appointed recovery container, then the rotating disc drives the reaction cup to rotate a certain angle, the liquid suction needle is located in a gap between two mounting holes, cleaning liquid is injected into the inner liquid suction needle 5.4 and the outer cleaning needle 5.3 at the same time by the cleaning system, the length of the inner liquid suction needle 5.4 is longer than that of the outer cleaning needle 5.3, the cleaning liquid in the outer cleaning needle 5.3 flows out of the inner liquid suction needle 5.4 through the outlet of the inner liquid suction needle, the cleaning effect of the outer wall of the inner liquid suction needle 5.4 is guaranteed, the inner wall of the inner liquid suction needle 5.4 is cleaned in the downward flowing process of the cleaning needle, the cleaning waste liquid directly flows into the guide groove 4.4, cross contamination is completely avoided, and the accuracy of a detection result is improved.
Of course, during actual installation, the liquid suction needle can also adopt a formula sub-needle as shown in fig. 8 and 9, the formula sub-needle comprises a needle tube 6.1 fixedly connected to the mounting seat 3.1, a cleaning pool 6.3 vertically corresponding to the needle tube 6.1 is arranged above the magnetic cleaning assembly through a connecting piece 6.2, a pair of perforations matched with the needle tube 6.1 are formed in the top wall and the bottom wall of the cleaning pool 6.3, a water inlet joint 6.4 is arranged on the left side wall of the cleaning pool 6.3, and a water outlet joint 6.5 is arranged on the right side wall of the cleaning pool 6.3. When the waste sample is extracted, the needle tube 6.1 of the needle is inserted into the sample waste liquid in the reaction cup through the cleaning pool 6.3 along with the head washing component to be lowered to a preset height, the liquid pumping system is used for sucking the sample waste liquid into the specified recovery container, the rotating disk drives the reaction cup to rotate for a certain angle, the needle is located in a gap between two mounting holes, the cleaning system simultaneously injects cleaning liquid into the cleaning pool 6.3 and the needle tube 6.1, the cleaning liquid in the cleaning pool 6.3 in the ascending process of the needle tube cleans the outer wall of the needle tube 6.1, the cleaning system stops working after the needle tube ascends to a preset position, and the cleaning waste liquid flows into the guide groove 4.4 in the cleaning process, so that the needle tube has good cleaning effect, can avoid sample wall hanging, and further cross contamination is avoided.
When the magnetic bead pre-adsorption device works, the power source drives the rotary disk 4.1 to rotate, the reaction cup rotates along the guide groove 4.4 and rotates from the non-magnet area to the pre-adsorption position corresponding to the pre-adsorption position magnet 4.5, and after the reaction cup is stationary at the pre-adsorption position for a preset time, the magnetic beads are attached to the inner surface of the reaction cup close to the outer side wall of the guide groove 4.4, so that the pre-adsorption of the magnetic beads is realized; the rotating disc 4.1 drives the reaction cup to rotate to a liquid pumping level, the magnetic beads are transferred to the surface of the reaction cup close to the inner side wall of the guide groove 4.4 under the action of the liquid pumping level magnet 4.6, the hair washing assembly descends to a designated position, the inner cleaning needle of the sleeve needle is inserted into sample waste liquid in the reaction cup, the sample waste liquid is pumped into a designated recovery container, then the rotating disc 4.1 rotates for a certain angle, the cleaning system cleans the sleeve needle, after the sleeve needle is cleaned, the rotating disc 4.1 drives the reaction cup to move to the position corresponding to the liquid injection level magnet 4.7 of the first group of magnet group, cleaning liquid is injected into the reaction cup through the liquid injection needle 3.2, the rotating disc 4.1 drives the reaction cup to move to the position corresponding to the liquid suction level magnet 4.8 of the first group of magnet group, and the magnetic beads are adsorbed on the inner surface of the reaction cup close to the outer side wall of the guide groove 4.4 after the reaction cup is stationary for a preset time, so that one-time injection-suction operation of the magnetic beads in the reaction cup is realized; the method is repeated for five times, realizes five times of injection-suction operation of the magnetic beads, and has good cleaning effect. Of course, according to the actual requirement, the magnetic beads can be injected and sucked once or twice or three times or four times after the sample waste liquid is extracted.