CN109762795A - A Drought Resistance-Related Sesame Gene SiGolS2 and Its Application - Google Patents

Abstract

The invention discloses a kind of Sesame SiGolS2 relevant to drought resisting and its applications.A kind of relevant sesame (Sesamum indicum L.) the gene SiGolS2 of drought resisting, nucleotides sequence is classified as shown in SEQ ID NO.1, or the nucleotide sequence with same function to be generated by the sequence addition, substitution, insertion or deletion one or more nucleotide.By the overexpression by sesame gene related to drought tolerance SiGolS2 in arabidopsis, it is found that this gene can significantly improve the drought resistance of arabidopsis, has preferable application prospect applied to crop drought resistance breeding.

Description

A kind of relevant Sesame SiGolS2 of drought resisting and its application

Technical field

The invention belongs to molecular biology fields, and in particular to a kind of Sesame SiGolS2 relevant to drought resisting, simultaneouslyFurther relate to application of the gene in crop drought resistance breeding.

Background technique

Sesame (Sesamum indicum L.) is the characteristic oil crops in China, and cultivation history is remote, throughout the country each, worldwide there is a plantation on ground, especially more in Asia and Africa plantation, and China is main producing country and trading country,But it is self-supporting insufficient.In recent years, the yield decline of China's sesame is serious, this and sesame are in growth and development process by arid, waterThe influence of all kinds of abiotic stress such as flooded and saline and alkaline has a very large relationship, wherein arid is exactly a big key factor.SesameIndustry's technology system once found that China's sesame was throughout the year by drought stress shadow to the investigation and analysis of China major part sesame main producing regionIt rings, it is the major reason for causing sesame Severe Reduction that arid, which has seriously threatened growth and development, the increase of production of sesame,.In addition,The sesame production area of the most countries such as Africa and South Asia, Sesame Seed Yield is equally just by the threat of arid.Therefore, sesame is improvedNumb drought resistance promotes stable high yield, to promotion China's sesame production development, solves the problems, such as that total amount is self-supporting insufficient particularly important.MeshThe preceding research about sesame gene related to drought tolerance function is also deficienter, and the relevant research report of molecule is also relatively fewer, thereforeFocus on excavating sesame drought resisting functional gene, be of great significance to carrying out sesame drought resisting molecular breeding, improving sesame drought-resistant ability.

Plant will receive the influence of many environmental factors often in growth and development, and when these stress occur, plant canWith a large amount of stress response gene of acute activation and synthesize various functional proteins, by formed a series of complex signal network comeUnfavorable environment is resisted, its own normal physiological function is maintained, achievees the purpose that evade stress damage.Plant is by dryUnder the water stress that a variety of adverse circumstances such as drought, high/low temperature, salt marsh are formed, some osmotic adjustments can be actively accumulated to improve in vivoCell liquid concentration reduces osmotic potential, cell water suction or water holding capacity is improved, to adapt to the environment of water stress.Gossypose systemColumn oligosaccharides (RFOs) is one of small point of osmotic adjustment, and some researches show that some extremely low without RFOs or content in vivoPlant, can be accumulated rapidly by RFOs after above-mentioned environment stress, plant resistance also enhances therewith, the life of this response adverse circumstancePhenomenon is managed in tomato (Downie et al., 2003), rice (Tacahashi et al., 1994), clover (CunninghamEt al., 2003), capsicum (Miao Min jade-like stone etc., 2008) etc. exist in plants.And it studies, it was also found that galactinol synthesizesEnzyme (Galactinol synthase, GolS) is the key enzyme in gossypose synthesis, therefore it is coerced in plant reply up to passIt is important.Most of GolS genes are stimulated by various stress and are induced.7 GolS genes (AtGolS1-7) are identified in arabidopsis.Wherein, induction of the expression of AtGolS1 and AtGolS2 by arid and salt stress, the quasi- south of AtGolS1 or AtGolS2 overexpressionMustard material is significantly enhanced the tolerance of the stress such as arid, salt.In addition, model clean equal (2015) is it has also been found that cassava MeGolS5 baseThe expression quantity of cause rises rapidly under drought stress, and the overexpression gene can improve drought resisting in Escherichia coli and arabidopsisAbility illustrates that galactinol synthase plays an important role in plant drought adjusting.Sesame is important oil crops,In today that arid takes place frequently, cultivates drought-enduring sesame variety and have great importance.Therefore, from sesame un-mixing bases because and identifyingThe function that it is played in terms of drought resistance will have very important significance for cultivating degeneration-resistant Sesame Cultivar.

Summary of the invention

Technical problem to be solved by the invention is to provide a kind of relevant sesame of drought resisting (Sesamum indicum L.)Gene SiGolS2, coding albumen and application.

In order to solve the above technical problems, The technical solution adopted by the invention is as follows:

It provides a kind of drought resisting relevant sesame (Sesamum indicum L.) gene SiGolS2, there is such as SEQ IDNucleotides sequence shown in NO.1 is classified as or the sequence is added, is replaced, is inserted into or lacked one or more nucleotide and generateNucleotide sequence with same function.Pass through the overexpression by drought resisting correlation Sesame SiGolS2 in arabidopsis, hairThis existing gene is remarkably improved the drought-resistant ability of plant, provides genetic resources for plant drought breeding.

The invention also includes a kind of sesame SiGolS2 albumen is provided, there is the amino acid sequence as shown in SEQ ID NO.2Column or the sequence are through replacement, missing or one or several amino acids formed amino acid sequences with same function of addition.

The present invention include obtain by various methods, the amino acid sequence as shown in SEQ ID NO.2 or the sequence be through replacingIt changes, lack or adds one or several amino acids formed derived proteins with same function and encode these and derive eggThe gene of white matter.

The invention also includes the cloning vector containing SiGolS2 nucleotide sequence or all kinds of expression vectors, contain the loadThe host cell of body.

By SiGolS2, gene constructed to expression vector pCAMBIA1301S, (pCAMBIA1301S is in the world to the present inventionIt is reconstructed on the basis of common Genetic Transformation in Higher Plants carrier pCAMBIA1301, carrying has composing type and overexpression featureThe genetic transformation carrier of cauliflower mosaic virus CaMV35S promoter).It, will by Agrobacterium-medialed transformation methodThe SiGolS2 gene that pCAMBIA1301S is carried is transferred to arabidopsis, obtains transformation of Arabidopsis thaliana plant.The experimental results showed thatSiGolS2 has the function of improving plant drought resistance.

The present invention also provides above-mentioned SiGolS2 gene, carrier or host cells to improve the application in plant drought resistance.

Compared with the prior art, the advantages of the present invention are as follows:

The present invention is to report drought resisting correlation Sesame SiGolS2 sequence for the first time both at home and abroad, its overexpression can mention at presentThe function of high plant drought resistance does not have relevant report, our experiments show that: this gene is remarkably improved plant drought ability, is applied toCrop drought resistance breeding.Shown by overexpression experiment of the drought resisting correlation Sesame SiGolS2 in arabidopsis: transgenosis is quasi-The expression of SiGolS2 has significant improvement compared with receptor compares (nontransgenic plants) in southern mustard, Arabidopsis plantTherefore drought-resistant ability is also improved.Therefore the present invention proposes that the drought resisting of plant can be improved using the overexpression of SiGolS2Thus ability improves crop drought resistance, guarantees high crop yield stable yields for use in the breeding of oil crops drought-resistant variety.

Detailed description of the invention

Fig. 1 is expression of the SiGolS2 gene under a variety of adverse circumstances.Each processing sample are as follows: drought stress (15%The simulation of PEG6000 solution) processing 0h, 2h, 6h, 12h；High salt treatment 0h, 2h, 6h, 12h.

Fig. 2 is the building of the plant expression vector pCAMBIA1301S-SiGolS2 of the gene containing SiGolS2；

Fig. 3 is that pCAMBIA1301S-SiGolS2 converts Escherichia coli, the positive colony institute upgrading grain sequencing result of acquisitionWith SiGolS2 full length gene CDS sequence alignment schematic diagram (1: sequencing result；2:SiGolS2 full length gene CDS sequence)；

Fig. 4 turns SiGolS2 gene arabidopsis T1 for the selection result schematic diagram of positive plant；

Fig. 5 turns the PCR qualification result schematic diagram (M:Marker in SiGolS2 gene arabidopsis T1 generation；1-9: transgenosis T1 generationPlant；WT: wildtype Arabidopsis thaliana)；

Fig. 6 turns SiGolS2 gene arabidopsis T2 for the selection result schematic diagram of positive plant；

Fig. 7 turns the PCR qualification result schematic diagram (M:Marker in SiGolS2 gene arabidopsis T2 generation；1-5: transgenosis T2 generationPlant；WT: wildtype Arabidopsis thaliana)；

Fig. 8 turns SiGolS2 gene arabidopsis T2 for plant quantitative expression verification result figure；

The measurement of Fig. 9 antecedent soil moisture method turns SiGolS2 gene arabidopsis T2 for the drought resistance of plant.

Specific embodiment

Following embodiment defines the present invention, and describing the present invention in clone includes that SiGolS2 gene completely encodesThe DNA fragmentation of section, and the method for verifying SiGolS2 gene function.

The method for cloning the relevant gene SiGolS2 of heretofore described drought resisting be in this field commonly used by method.The method of mRNA is extracted also there are many mature technology, kit (EASY spin Plus Plant RNA kit) can be used,It commercially obtains and (is purchased from Aidlab Biotechnologies Co., Ltd).Construct heretofore described carrierWith carrier is transferred to digestion used in plant, connection, inflorescence the methods of infect is also technology commonly used in the art.It is wherein involvedAnd plasmid (pCAMBIA1301), transfection use media (agrobacterium tumefaciens lba4404 and agents useful for same ingredient such as sucrose, Kan, tideMycin etc.) commercially obtain.

The acquisition of embodiment 1:SiGolS2 gene

It is compared within the scope of sesame full-length genome by homology sequence using the protein sequence of arabidopsis GolS geneAnalysis, identification obtains 7 sesame GolS genes, including SiGolS2 gene.

By searching for Sesame group database (http://ocri-genomics.org/Sinbase/Index.html), according to the CDS sequence of SiGolS2 gene, design can expand the primer of its complete CDS sequence.Take flowering stageTissue after the drought stress 7 days of drought-resistant variety Shanxi sesame No. 2 (is purchased from EASYspin Plus plant RNA extraction kitAidlab company), extract total serum IgE, using reverse transcriptase (be purchased from Vazyme company) by its reverse transcription synthesize cDNA (method according toVazyme company reverse transcriptase reagent specification).Using the cDNA after reverse transcription as template, the primer title (includes with sequenceModified base) as follows: SiGolS2FL-F:5 '-GCTTTCGCGAGCTCGGTACCATGGAAATGGAGTCTAAGGC-3 ', such asShown in SEQ ID NO.3；SiGolS2FL-R:5 '-CGACTCTAGAGGATCCTCAGGATGAGTCTTCATTGC-3 ', such as SEQShown in ID NO.4；

Being gone out with above-mentioned primer amplification includes the cDNA segment of SiGolS2 gene complete coding region, the segment that amplification is obtainedIt is sequenced, SiGolS2 gene order is obtained, as shown in SEQ ID NO.1.The amino acid sequence of protein encoded by it is such asShown in SEQ ID No.2.

The expression of the endogenous SiGolS2 gene of 2 sesame of embodiment

Sesame drought stress transcriptome analysis shows that SiGolS2 is expressed by drought stress induced strong, may have raisingThe function of sesame drought resistance.In order to further confirming that transcript profile as a result, applicant's SiGolS2 gene endogenous to sesame is inverseExpression under the stress of border is detected.

Applicant selects sesame variety " middle sesame 13 " (disclosure that Inst. of Oil Crops, Chinese Academy of Agriculture cultivatesThe sesame variety of popularization and application) material as expression pattern analysis.It grows to 4 leaf phase seedling and carries out various adverse circumstance processing.AridProcessing is simulated with the processing of 15%PEG6000 solution, 0h, 2h, is sampled after 6h, 12h；High-salt stress is to be immersed in seedling rootThe solution of 200mM NaCl, 0h, 2h are sampled after 6h, 12h.The extraction of total serum IgE EASYspin Plus plant RNA extraction reagentBox (be purchased from Aidlab company), using reverse transcriptase (being purchased from Vazyme company) by its reverse transcription synthesize cDNA (method according toVazyme company reverse transcriptase reagent specification).Using above-mentioned reverse transcription synthesis cDNA as template, with primer (SiGolS2-F:5 '-AAACTCTCCAAATCACTCCTCC-3 ', as shown in SEQ ID NO.5 and SiGolS2-R:5 '-GCCGGTATATCTCCCTAAAGAAC-3 ', as shown in SEQ ID NO.6) special PCR amplification is carried out to SiGolS2 gene.Primer (SiHF:5 '-GTTGGTCTCTTTGAGGAC-3 ', as shown in SEQ ID NO.7) and SiHR:5 '-are used simultaneouslyCAGCTGGATGTCTTTTGG-3 ', as shown in SEQ ID NO.8) sesame Histone H3.3 gene (SIN_1004293) is doneSpecific amplified, to carry out quantitative analysis as internal reference.Reaction condition are as follows: 95 DEG C of 30sec；95 DEG C of 10sec, 60 DEG C of 30sec, 35A circulation.Fluorescence detection real-time quantitative analysis is carried out in reaction process.The result shows that SiGolS2 gene (SEQ NO:1) is dryExpression quantity rising (Fig. 1) after drought (15%PEG6000 solution processing simulation) and high salt treatment illustrates that SiGolS2 gene may beIt improves and plays a significant role in sesame drought resistance.

A kind of embodiment 3: application of the Sesame SiGolS2 in plant drought breeding improving plant drought resistance

1) the relevant Sesame SiGolS2 of above-mentioned drought resisting is utilized into method and pCAMBIA1301S (this of homologous recombinationLaboratory provides) plasmid connection building plant expression vector, it is named as pCAMBIA1301S-SiGolS2 (Fig. 2), concrete operationsIt is as follows:

1. linearized vector is obtained first with double digestion (BamHI and KpnI) (Takara) method, it is solidifying by agaroseGel electrophoresis and plastic recovery kit (Tiangeng biochemical technology Co., Ltd) purifying obtain high-purity linearized vector.

Recombinate instead 2. target fragment DNA and linearized vector are added in the centrifuge tube of 1.5ml with the molar ratio of 3:1It answers, places about 30min after mixing in 37 DEG C of water-baths, the reaction solution that 10 μ L are added takes out to from -80 DEG C of refrigerators on iceIn the 50 μ L DH5a competent cells for melting 10min, pipettor is mixed gently, then at being incubated for 20min, 42 DEG C of water-bath heat on iceCooled on ice 2min is quickly set after swashing 45 seconds.

3. 300 μ L LB liquid mediums are added in clean bench, 37 DEG C of incubation 60min.13,000rpm is centrifuged 1min and collectsThallus abandons part supernatant, thallus is resuspended with remaining culture medium, is trained with sterile spreading rod in the LB solid containing Kan resistanceIt supports and is gently smoothened on base, culture 16-24h is inverted in 37 DEG C of incubators.

4. the clone of several recombining reactions of picking conversion carries out bacterium colony PCR identification, it will be accredited as each of the positive laterSingle colonie picks them separately in the LB liquid medium containing Kan antibiotic 37 DEG C, is incubated overnight in 200rpm incubator, mentionsTake plasmid, and corresponding bacterium solution direct Sequencing is identified into carrier accuracy.By sequence alignment (Fig. 3), sequencing result withSiGolS2 full length gene CDS sequence is completely the same, illustrates that the plant expression vector of building is accurate.

2) the carrier pCAMBIA1301S-SiGolS2 prepared in step 1) is transferred to agrobacterium tumefaciens lba4404 (ShanghaiWei Di Bioisystech Co., Ltd), then import in Arabidopsis plant, concrete operations are as follows:

(1) recombinant vector is transferred to Agrobacterium LBA4404:

1. 2 μ g Plasmid DNA are added in every 100 μ L LBA4404 Agrobacterium competent cell, it is mixed that tube bottom is gently dialed with handIt is even, successively in standing 5min, liquid nitrogen 5min, 37 DEG C of water-bath 5min, ice bath 5min on ice.

2. the LB liquid medium of 700 μ L antibiotic-frees is added, in 28 DEG C of shaken cultivation 5h.13,000rpm centrifugation 1minThallus is collected, 100 μ L or so supernatant is left and taken, gently thallus is resuspended in piping and druming, it is equably coated on to the LB containing Kan and RifOn solid medium, 28 DEG C culture carton upside down culture 2 days, several positive colonies of picking, with the primer in embodiment 2SiGolS2FL-F/R, which carries out bacterium colony PCR, can amplify target fragment, illustrate that carrier pCAMBIA1301S-SiGolS2 has been transferred toIn agrobacterium tumefaciens lba4404.

(2) the plate culture of arabidopsis:

1. needing to count a certain number of arabidopsis seeds loaded in sterile 1.5mL centrifuge tube according to experiment.

2. 75% ethyl alcohol of 1mL is added, mixing of turning upside down is abandoned supernatant, is repeated 1 times.It is put into 37 DEG C of 200rpm shaking tables and shakes10min surface sterilizing.

3. abandoning 75% ethyl alcohol, 95% ethyl alcohol of 1mL is added, mixing of turning upside down is abandoned supernatant, and is repeated 1 times.In ultra-clean workMake in platform, 300-500 μ L dehydrated alcohol is added in Xiang Geguan seed, is sprayed onto seed and dehydrated alcohol together with 1mL pipettorOn aseptic filter paper.

4. being evaporated completely to ethyl alcohol, the arabidopsis seed point done is sowed to the plate of ready MS culture medium with toothpickOn.

5. plate is sealed, it is inverted in 4 DEG C of vernalization 48h under dark condition, after vernalization, plate is placed in illumination cultivationIt is cultivated vertically in case, emergence can transplant after a week.

6. in the soil that seedling is planted to small basin with tweezers, first using preservative film moisturizing 48h, middle culture is straight between being placed in plant growthWhen growing bolting to arabidopsis (about one month), it to be used for transformation experiment.

(3) genetic transformation:

1. Agrobacterium activates: it is separately added into 20 μ L Rif and Kan in the LB liquid medium of 20mL, shakes up and connect bacterium,28 DEG C of 220rpm concussion activation 8-10h.

2. Agrobacterium expands culture: being separately added into 200 μ L Rif and Kan in the LB liquid medium of 200mL, addThe activation bacterium solution of 5-10mL, 28 DEG C of 220rpm shake culture 14-16h, until OD value 1.6-2.0,4500rpm are centrifuged 10min, precipitatingThallus abandons supernatant, naturally dry.

3. 5% sucrose solution of 100mL is added in precipitating thallus is resuspended thallus, pipettor piping and druming is uniform, thallus is resuspended.

4. bacterium solution in centrifugal bottle is added in plate, 5% sucrose solution of 100mL is added, 40 μ L are added before convertingSilwet-L-77 (0.02%) shakes plate and mixes.

5. arabidopsis floral is closed up, plate is immersed, 15s is shaked gently.

6. plant is packaged with black sack, be protected from light moisturizing for 24 hours.

7. it is primary to repeat conversion after a week.

3) screening T1 is for positive plant.

The seed that plantation arabidopsis T0 generation is harvested, is sterilized, is inoculated with the MS screening and culturing medium of the hygromycin containing 30mg/L(addition 25mg/L cephalosporin antibacterial) it is upper 22 DEG C illumination cultivation 7-10 days, (seedling and root system are all just for screening acquisition positive plantThe plant being frequently grown) (Fig. 4), this experiment obtains 6 positive strains, by positive transplantation of seedlings into soil, with 2-3 on preservative film coverFilm is taken off after it, then normal growth.After the leaf DNA of the positive plant filtered out is extracted, SiGolS2 is identified using PCR methodGene carries out the Molecular (Fig. 5) of the target gene of transgenic plant, final to confirm that gene has been transferred to the positive plant of T1 generationStrain.

4) transgenic plant T2 is for positive detection

T1 carries out single plant sowing for positive plant, and the T1 seed harvested for positive plant is continued hygromycin selection and is obtainedT2 for positive plant (plant of seedling and root system all normal growths) (Fig. 6), choose above-mentioned acquisition part positive strain system by itsTransplanting growth extracts leaves genomic DNA and carries out PCR Molecular Identification (Fig. 7), determines T2 for positive plant.

5) transgenic plant T2 is verified for positive plant quantitative expression

Transgenic plant T2 is taken to be mentioned for the young leaflet tablet of positive plant and wild-type Arabidopsis plants growth period with RNAKit (Beijing Ai Delai Biotechnology Co., Ltd) is taken to extract blade total serum IgE, then (Nanjing promise is only with reverse transcription reagent boxPraise Biotechnology Co., Ltd) cDNA is obtained, using respective cDNA as template, using arabidopsis β-actin as internal reference (aF:5 '-CCCGCTATGTATGTCGCCA-3 ', as shown in SEQ ID NO.9；AR:5 '-AACCCTCGTAGATTGGCACAG-3 ', such as SEQShown in ID NO.10), target gene quantifies primer sequence are as follows: SiGolS2-F:5 '-AAACTCTCCAAATCACTCCTCC-3 ',As shown in SEQ ID NO.5；SiGolS2-R:5 '-GCCGGTATATCTCCCTAAAGAAC-3 ', as shown in SEQ ID NO.6,Carry out qRT-PCR expression verifying (qRT-PCR Mix: Nanjing Vazyme Biotechnology Co., Ltd.；Instrument: Roche480).The result shows that being control with the wildtype Arabidopsis thaliana of non-transgenosis, sesame SiGolS2 gene is detecting3 arabidopsis transgenic lines blade in expression quantity significantly improve, and in the blade of non-non-transgenic control arabidopsis, do not haveThere is the expression (Fig. 8) for detecting sesame SiGolS2 gene.Show that the SiGolS2 gene of sesame is converted and is inserted into correspondingIn arabidopsis gene group and expressed.

6) T2 generation positive arabidopsis thaliana drought resistance measurement

It is wild to arabidopsis transgenic plant and non-transgenosis respectively to T2 for growth of transgenic plants to 3 pairs of leaf periodsType Arabidopsis plant carries out drought stress and handles 16 days, and discovery transgenic plant is withered with non-transgenosis wild-type Arabidopsis plantsIt is listless serious.After above-mentioned material is carried out rehydration processing 3 days, the results showed that compared with wildtype Arabidopsis thaliana control, this research institute is obtainedThe SiGolS2 gene arabidopsis strain that turns obtained is gradually brought to normal growing conditions, and wildtype Arabidopsis thaliana is then still in wiltingState illustrates that turning SiGolS2 gene arabidopsis strain transgenic line drought-resistant ability compares (Fig. 9) higher than wildtype Arabidopsis thaliana,Show that the drought-resistant ability of plant can be improved in the overexpression of sesame SiGolS2 gene.

SEQUENCE LISTING

<110>Inst. of Oil Crops, Chinese Academy of Agriculture

<120>a kind of relevant Sesame SiGolS2 of drought resisting and its application

<160> 10

<170> PatentIn version 3.5

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<213> Sesamum indicum

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atggaaatgg agtctaaggc caacagggct tatgtcacat tcttggcagg aaacggtgac 60

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cttgttgtag cagttttgcc agatgttcca gtagatcatt gcaagatttt agagtctcaa 180

gggtgtatag ttcgtgaaat cgagcctgtt tatcctccac caaaccatgc tcaatttgcc 240

atggcatatt atgtcataaa ctattcaaaa ctccggattt gggagtttgt ggagtatagc 300

aagatgatat acttggatgg agatatccaa gttttcagca atattgatca cctttttgag 360

tatccagatg gttattttta tgctgttatg gactgcttct gtgagaaaac atggagtgga 420

acacctcaat accagatagg ttactgtcag cagtgccctg aaaaagttga gtggccatgg 480

gagatgggtc ctccaccacc attgtacttc aatgccggaa tgtttgtttt cgaaccgagc 540

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gagcaggact ttctgaacat gttctttagg gagatatacc ggccaatccc attggtttac 660

aaccttgttt tagctatgct ttggcgacat cctgaaaatg ttgatcttga caatgtcaag 720

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atgcaaagag aagacataaa gatgctagtg aaaaaatggt gggatatata ccgtgatgat 840

tcgttggatt ggtgcaatga agactcatcc tga 873

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Claims (7)

Translated fromChinese

1.一种抗旱相关的芝麻基因SiGolS2，其特征在于：其具有如SEQ ID NO.1所示的核苷酸序列，或该序列经添加、取代、插入或缺失一个或多个核苷酸而产生的具有同等功能的核苷酸序列。1. a sesame gene SiGolS2 related to drought resistance, is characterized in that: it has the nucleotide sequence as shown in SEQ ID NO.1, or this sequence is through adding, replacing, inserting or deleting one or more nucleotides and The resulting nucleotide sequence is functionally equivalent.2.权利要求1所述的芝麻基因SiGolS2编码的芝麻SiGolS2蛋白，其特征在于：其具有如SEQ ID NO.2所示的氨基酸序列或该序列经替换、缺失或添加一个或几个氨基酸形成的具有同等功能的氨基酸序列。2. the sesame SiGolS2 protein encoded by the sesame gene SiGolS2 of claim 1, is characterized in that: it has the aminoacid sequence shown in SEQ ID NO.2 or the sequence formed by replacing, deleting or adding one or several amino acids functionally equivalent amino acid sequence.3.含有权利要求1所述的芝麻基因SiGolS2核苷酸序列的克隆载体或各类表达载体。3. the cloning vector or various expression vectors containing the sesame gene SiGolS2 nucleotide sequence of claim 1.4.含有权利要求3所述载体的宿主细胞。4. A host cell comprising the vector of claim 3.5.权利要求1所述的芝麻基因SiGolS2在提高植物抗旱性的应用。5. the application of the sesame gene SiGolS2 described in claim 1 in improving plant drought resistance.6.权利要求3所述的克隆载体或各类表达载体在提高植物抗旱性中的应用。6. Application of the cloning vector or various expression vectors of claim 3 in improving plant drought resistance.7.权利要求4所述的宿主细胞在提高植物抗旱性中的应用。7. The application of the host cell of claim 4 in improving plant drought resistance.