Summary of the invention
The purpose of the present invention is to overcome the defects in the prior art, provides a kind of BOD Quick testing instrument and accurate benefitMeasuring method is repaid, the Fast And Accurate Determination of low concentration BOD surface water is particularly suitable for.
The present invention to achieve the above object, using following technical scheme:
A kind of BOD Quick testing instrument, including dissolved oxygen sensing module, BOD microorganism measurement module and processing module；InstituteThe dissolved oxygen sensing module stated includes sample injection unit and cleaning unit；The sample injection unit includes sequential communication for holdingReceive sample injector, sample feeding pipe for sampling, a branch of threeway, peristaltic pump and the flow type dissolved oxygen sensing instrument of sample；The cleaning unit includes cleaning solution bucket, cleaning liquid pipe and another branch of threeway of sequential communication；The threewayAnother branch be connected to the peristaltic pump；The BOD microorganism measurement module includes dissolving with the flow typeThe thermostat of oxygen measuring device sequential communication, a branch of gas-liquid mixed threeway, flow type BOD microbiological sensor, waste liquidPipe and waste liquid barrel；Another branch of the gas-liquid mixed threeway is connected to by tracheae with air pump.
Solenoid valve is provided on the sample feeding pipe and cleaning liquid pipe.
The processing module includes main control computer and the online display interface of main control computer and signal measurement and processingUnit；The signal measurement and processing unit and the flow type dissolved oxygen sensing instrument and flow type BOD microorganism passSensor connection；The main control computer is connect with controller；The controller stated is used to control the on-off of the solenoid valve, wrigglesThe sampling of the switch and sample injector of pump.
The dissolved oxygen sensor of the flow type dissolved oxygen sensing instrument can be polarogram formula overlay film oxygen electrode or fluorescence methodOxygen electrode.
Flow type BOD microbiological sensor is made of polarogram formula three-electrode structure oxygen electrode with sandwich-type microbial film；The electrode group of the polarogram formula three-electrode structure oxygen electrode becomes: working electrode: gold；To electrode: platinum；Reference electrode: silver-chlorineChange silver.
The application further includes the accurate compensation measuring method of BOD Quick testing instrument described in one kind, is dissolved using flow typeThe measuring signal of oxygen measuring device and Formula B OD microbiological sensor utilizes the BOD value of formula judgement sample；The formulaAre as follows:
BOD=F (IA)+F(△I)
F(IA)=a5×IA5+a4×IA4+a3×IA3+a2×IA2
F (△ I)=k × △ I+b
IA=△ D- △ I；
Wherein:
IA is the difference that flow type dissolved oxygen instrument electrode measurement potential drop and flow type BOD microbiological sensor measure potential dropValue.
A5 is potential value at the end of the measurement of flow type BOD microbiological sensor；
A4 is potential value at the end of flow type dissolved oxygen sensing instrument electrode measurement；
A3 is potential value when flow type BOD microbiological sensor initial measures；
A2 is potential value when flow type dissolved oxygen sensing instrument electrode initial measures；
△ D is flow type dissolved oxygen sensing instrument electrode measurement whole story current potential depreciation；
△ I is that flow type BOD microbiological sensor measures whole story potential difference；
K, b is coefficient of standard curve；
The value range of k value is 11.77-13.35；
The value range of b value is 2.26-2.78.
Compared with prior art, the beneficial effects of the present invention are:
Previous BOD Quick testing instrument works as sample simply by the BOD value in microbiological sensor sample solutionWhen the dissolved oxygen of itself changes in solution, error will be brought to BOD measurement, and present invention introduces flow type dissolved oxygen sensingsThe dissolved oxygen compensating module of instrument, can effectively eliminate above-mentioned error, and give equation of higher degree compensated curve, improve BOD measurementPrecision and accuracy, the measurement especially for low concentration surface water BOD is possessed of higher values.
Detailed description of the invention
Fig. 1 is the overall structure diagram of BOD Quick testing instrument of the present invention；
Fig. 2 is the partial structural diagram of BOD Quick testing instrument of the present invention；
In figure: 1, sample injector 2, cleaning solution bucket 3, sample feeding pipe 4, cleaning liquid pipe
5, solenoid valve 6, threeway 7, peristaltic pump 8, flow type dissolved oxygen sensing instrument
9, thermostat 10, air pump 11, flow type BOD microbiological sensor
12, waste liquid barrel 13, main control computer 14, display interface 15, solenoid valve fixture block
16, sewer pipe 17, electromagnetism valve retainer 18, pump line 19, liquid pipe
20, gas-liquid mixed threeway 21, tracheae 22, signal measurement and processing unit.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, with reference to the accompanying drawing and mostThe present invention is described in further detail for good embodiment.
Fig. 1-2 shows a kind of BOD Quick testing instrument, including dissolved oxygen sensing module, BOD microorganism measurement module and placeManage module；The dissolved oxygen sensing module includes sample injection unit and cleaning unit；The sample injection unit includes that sequence connectsThe logical sample injector 1 for accommodating sample, sample feeding pipe for sampling 3, threeway 6 a branch, peristaltic pump 7 and flow typeDissolved oxygen sensing instrument 8；The cleaning unit include sequential communication cleaning solution bucket 2, cleaning liquid pipe 4 and threeway 6 it is anotherA branch；Another branch of the threeway 6 is connected to the peristaltic pump 7；The BOD microorganism measurement module includesWith branch, a flow type of the thermostat 9 of the flow type dissolved oxygen sensing instrument sequential communication, gas-liquid mixed threeway 20BOD microbiological sensor 11, sewer pipe 16 and waste liquid barrel 12；Another branch of the gas-liquid mixed threeway 20 passes throughTracheae 21 is connected to air pump 10.Solenoid valve 5 is provided on the sample feeding pipe and cleaning liquid pipe.The threeway and describedIt is connected between peristaltic pump by pump line 18；Pass through liquid between the flow type dissolved oxygen sensing instrument and the thermostat 9Pipe 19 is connected to.
The processing module includes main control computer and the online display interface of main control computer and signal measurement and processingUnit 22；The signal measurement and processing unit and the flow type dissolved oxygen sensing instrument and flow type BOD microorganismSensor connection；The main control computer is connect with controller；The controller be used to control the solenoid valve on-off,The sampling of the switch and sample injector of peristaltic pump.
The dissolved oxygen sensor of the flow type dissolved oxygen sensing instrument can be polarogram formula overlay film oxygen electrode or fluorescence methodOxygen electrode.
Flow type BOD microbiological sensor is made of polarogram formula three-electrode structure oxygen electrode with sandwich-type microbial film；The electrode group of the polarogram formula three-electrode structure oxygen electrode becomes: working electrode: gold；To electrode: platinum；Reference electrode: silver-chlorineChange silver.
A kind of accurate compensation measuring method of Quick testing instrument, it is micro- using flow type dissolved oxygen sensing instrument and Formula B ODThe measuring signal of biosensor utilizes the BOD value of formula judgement sample；The formula are as follows:
BOD=F (IA)+F(△I)
F(IA)=a5×IA5+a4×IA4+a3×IA3+a2×IA2
F (△ I)=k × △ I+b
IA=△ D- △ I
Wherein:
IAThe difference of potential drop is measured for flow type dissolved oxygen instrument electrode measurement potential drop and flow type BOD microbiological sensorValue.
A5 is potential value at the end of the measurement of flow type BOD microbiological sensor；
A4 is potential value at the end of flow type dissolved oxygen sensing instrument electrode measurement；
A3 is potential value when flow type BOD microbiological sensor initial measures；
A2 is potential value when flow type dissolved oxygen sensing instrument electrode initial measures；
△ D is flow type dissolved oxygen sensing instrument electrode measurement whole story current potential depreciation；
△ I is that flow type BOD microbiological sensor measures whole story potential difference；
K, b is coefficient of standard curve；
The value range of k value is 11.77-13.35；
The value range of b value is 2.26-2.78.
The course of work of the invention are as follows: set and show data and instrument real-time status in display interface 14.Main control computerThe automatic positioning and sample introduction switching of 13 control sample injectors 1 (being full-automatic sample injector in the present invention) and its solenoid valve 5.When booting,Main control computer 13 controls full automatic sample injector 1 and resets, and makes positioning to No. 1 position, while controlling the opening of solenoid valve 5, when openingSolenoid valve fixture block 15 and electromagnetism valve retainer 17 clamp sample feeding pipe 3, and sample feeding pipe 3 is made to be in closed state, and cleaning liquid pipe 4 is in circulationState.Peristaltic pump 7 rotates, and extracts cleaning solution from cleaning solution bucket 2 by cleaning liquid pipe 4 and is sent into pump line 18 to stream by threeway 6The import of general formula dissolved oxygen sensing instrument 8, main control computer 13 controls signal measurement and processing unit 22 acquisition flow type in real time at this timeThe data of dissolved oxygen measuring device 8 carry out the dissolved oxygen parameter that processing obtains cleaning solution, and liquid is again by flow type dissolved oxygen sensingThe outlet of device 8 enters liquid pipe 19, later from the import of thermostat 9 enter, constant temperature heat preservation after from the outlet of thermostat 9 intoEnter with to the gas-liquid mixed threeway 20 connecting, is mixed with from the air in the tracheae 21 that air pump 10 is connect, gas-liquid mixed liquor entersThe import of flow type BOD microbiological sensor 10, main control computer 13 controls signal measurement at this time and processing unit 22 acquires in real timeThe data of flow type BOD microbiological sensor 10 are handled, and the corresponding data of cleaning solution are obtained.Treated cleaning solution is from streamThe outlet of Formula B OD microbiological sensor 10 enters the measurement process that waste liquid barrel 12 completes cleaning solution by sewer pipe 16.
After main control computer 13 clocks between when purged, main control computer 13 controls full-automatic sample injector 1 and positions in order,The closure of solenoid valve 5 is controlled simultaneously, and solenoid valve fixture block 15 clamps cleaning solution 4 with electromagnetism valve retainer 17 when closure, is in cleaning solution 4Closed state, sample feeding pipe 3 are in circulation status.Peristaltic pump 7 rotates, by sample feeding pipe 3 from the corresponding position of full-automatic sample injector 1Pipette samples water sample is sent into pump line 18 to the import of flow type dissolved oxygen measuring device 8, at this time main control computer 13 by threeway 6The data of acquisition flow type dissolved oxygen measuring device 8 carry out handling acquisition water sample in real time for control signal measurement and processing unit 22Dissolved oxygen parameter, liquid enters liquid pipe 19 by the outlet of flow type dissolved oxygen measuring device 8 again, later from thermostat 9 intoMouth enters, and from the outlet of thermostat 9 entrance and the gas-liquid mixed threeway 20 to connection after constant temperature heat preservation, connect with from air pump 10Tracheae 21 in air mixing, gas-liquid mixed liquor circulates the import of formula BOD microbiological sensor 10, at this time main control computerThe data that 13 control signal measurements and processing unit 22 acquire flow type BOD microbiological sensor 10 in real time are handled, and are obtainedThe corresponding data of sample.Sample aqueous that treated from the outlet of flow type BOD microbiological sensor 10 by sewer pipe 16 intoEnter the measurement process that waste liquid barrel 12 completes cleaning solution.It circuits sequentially above step and carries out full-automatic sample introduction acquisition water sample data.
BOD Quick testing instrument according to the present invention, by dissolved oxygen sensing signal IAAnd BOD microbiological sensor signal IBCentral processing unit is accessed, this two paths of signals is subjected to calculation processing respectively, through a large amount of standard sample experimental data and realityThe control experiment of sample and traditional biochemical method on the five, establishes following empirical equation:
BOD=F (IA)+F(△I)
F(IA)=a5×IA5+a4×IA4+a3×IA3+a2×IA2
F (△ I)=k × △ I+b
IA=△ D- △ I
Wherein:
IAPotential drop is measured for dissolved oxygen electrode and BOD microbiological sensor measures the difference of potential drop.
a5Potential value at the end of for the measurement of BOD microbiological sensor.
a4Potential value at the end of for dissolved oxygen electrode measurement.
a3Potential value when BOD microbial membrane sensor initial measures.
a2Potential value when being measured for dissolved oxygen electrode initial.
△ D is that dissolved oxygen electrode measures whole story current potential depreciation.
△ I is that BOD microbial membrane sensor measures whole story potential difference.
K, b is coefficient of standard curve.
Above-mentioned F (IA) and F (IA) function is fitted all in accordance with measured data (being shown in Table 1.) by MATLAB and equation regression calculatesIt determines.K, the value of b slightly has difference according to the difference of water sample to be measured, here according to practical application experience, is divided into surface water, lifeThree kinds of main Types of sewage and industrial wastewater.
The value range of k value is 11.77-13.35
The value range of b value is 2.26-2.78
Table 1
Wherein, BOD5 value is the numerical value measured using traditional biochemical methods on the five, as a result registers, uses the present processesObtained BOD measured value infinite approach, and can satisfy the demand of automation, measurement is convenient, accurately, easy to spread.
The above is only a preferred embodiment of the present invention, for those of ordinary skill in the art, according to the present inventionThought, there will be changes in the specific implementation manner and application range, and the content of the present specification should not be construed as to the present inventionLimitation.