A kind of minigene detectorTechnical field
The present invention relates to detecting instrument field, in particular to a kind of minigene detector.
Background technique
After more than 20 years, the means of amplification were increasingly advanced for the development of gene amplification technology, and the quality of analysis detection is got overCome with precisely.Currently, the progress in terms of technique of gene detection is still continuing to bring out.Quickly, analysis method in place in real time,Biological sample genetic analysis will be studied and better help is provided.Molecular biology expert is also further pushing in-situ studyAuthenticity.People, which not only want to go to, to be amplified bio-genetic material to come, but also starts to become more concerned with the timeliness of detection.ButIt is, currently, the fields such as clinical gene detection, food safety, criminal investigation legal medical expert are also only at laboratory testing stage, mesh both at home and abroadMany emergency event timeliness are seemed more important by preceding increasingly complicated world structure and social situations, such as epidemic-stricken area diseaseThe early diagnosis of toxoinfection disease, the on-site test of food safety, criminal investigation legal medical expert quickly solve a case etc., for this purpose, it is proposed thatA kind of minigene detector.
Summary of the invention
The main purpose of the present invention is to provide a kind of minigene detectors, gene-detecting apparatus volume compression have been arrived non-Often small size, is become portable instrument, real-time Amplification Analysis can be carried out in field, without carrying bulky liquid nitrogen againTank carries out Sampling hold, can effectively solve the problems in background technique.
To achieve the above object, the technical scheme adopted by the invention is as follows:
A kind of minigene detector, including fuselage cover and pedestal, the fuselage cover are mounted on the base, the machineIntegrated heat conduction mechanism, control system, high-precision semiconductor temperature control member and CCD camera, institute are equipped in body shellIt states integrated heat conduction mechanism to overlay on high-precision semiconductor temperature control member, and high-precision semiconductor temperature controls structurePart side is equipped with control system, and the high-precision semiconductor temperature control member other side is equipped with highly sensitive fluorescence detector,And highly sensitive fluorescence detector side is electrically connected CCD camera, the CCD camera is connect with control system by conducting wire,One end of the high sensitivity fluorescence detector connects optical fiber, and optical fiber passes through high-precision semiconductor temperature control member and oneChange heat conduction mechanism.
Further, portable electric source mechanism, and portable power machine are equipped with above the highly sensitive fluorescence detectorThe portable power described in multiple groups forms in structure.
Further, the portable electric source mechanism and integrated heat conduction mechanism, control system, high-precision semiconductorTemperature control member, conductor temperature controller are connected with CCD camera by conducting wire.
Further, the surface of the integrated heat conduction mechanism is equipped with sample cell, and sample is placed in sample cellPipe, it is described integration heat conduction mechanism be made of Heat Conduction Material and frame, and Heat Conduction Material be filled in it is in frame and thermally conductiveMaterial is centered around around sample cell.
It further, include conductor temperature controller in the high-precision semiconductor temperature control member.
Compared with prior art, the invention has the following beneficial effects: by gene-detecting apparatus volume compression to very smallSize, novelty use high-sensitive CCD camera and carry out result differentiation, become portable instrument, no longer needed to sampleLaboratory is taken back, real-time Amplification Analysis can be carried out in field, Sampling hold is carried out without carrying bulky liquid nitrogen container again, by light, molecular biology, informatics etc. are effectively combined together, more convenient.
Detailed description of the invention
Fig. 1 is a kind of overall structure diagram of minigene detector of the present invention.
In figure: 1, fuselage cover；2, portable electric source mechanism；3, portable power；4, integrated heat conduction mechanism；5,Coupon；6, sample cell；7, control system；8, high-precision semiconductor temperature control member；9, conductor temperature controller；10,Pedestal；12, optical fiber；13, highly sensitive fluorescence detector；14, CCD camera；15, Heat Conduction Material；16, frame.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference toSpecific embodiment, the present invention is further explained.
As shown in Figure 1, a kind of minigene detector, including fuselage cover 1 and pedestal 10, the fuselage cover 1 are installedOn pedestal 10, integrated heat conduction mechanism 4, control system 7, high-precision semiconductor temperature are equipped in the fuselage cover 1Control member 8 and CCD camera 14, the integration heat conduction mechanism 4 overlay high-precision semiconductor temperature control member 8On, and 8 side of high-precision semiconductor temperature control member is equipped with control system 7, the high-precision semiconductor temperature control member 8The other side is equipped with highly sensitive fluorescence detector 13, and highly sensitive 13 side of fluorescence detector is electrically connected CCD camera 14,The CCD camera 14 is connect with control system 7 by conducting wire, and one end of the high sensitivity fluorescence detector 13 connects optical fiber12, and optical fiber 12 passes through high-precision semiconductor temperature control member 8 and integrated heat conduction mechanism 4.
In the present embodiment (as shown in Figure 1), gene-detecting apparatus volume compression very small size is arrived into, has been become justFormula instrument is taken, real-time Amplification Analysis can be carried out in field, carries out Sampling hold without carrying bulky liquid nitrogen container again.
Wherein, portable electric source mechanism 2, and portable electric source mechanism are equipped with above the highly sensitive fluorescence detector 13The portable power 3 described in multiple groups forms in 2.
In the present embodiment (as shown in Figure 1), convenient for being dismantled.
Wherein, the portable electric source mechanism 2 and integrated heat conduction mechanism 4, control system 7, high-precision semiconductorTemperature control member 8, conductor temperature controller 9 are connected with CCD camera 14 by conducting wire.
In the present embodiment (as shown in Figure 1), power supply is provided for each component.
Wherein, the surface of the integrated heat conduction mechanism 4 is equipped with sample cell 6, and is placed with coupon in sample cell 65, the integration heat conduction mechanism 4 is made of Heat Conduction Material 15 and frame 16, and Heat Conduction Material 15 is filled in frame 16,And Heat Conduction Material 15 is centered around around sample cell 6.
In the present embodiment (as shown in Figure 1), temperature is quickly transmitted in coupon 5, to be reacted.
It wherein, include conductor temperature controller 9 in the high-precision semiconductor temperature control member 8.
In the present embodiment (as shown in Figure 1), temperature control is carried out using conductor temperature controller 9.
It should be noted that the present invention is a kind of minigene detector, it, will be in portable electric source mechanism 2 when workThe opening of portable power 3 powers on, and is issued and is instructed by control system 7, and half in high-precision semiconductor temperature control member 8Conductor temperature controller 9 carries out temperature program(me) variation according to instruction, is ultimately transferred to try using integrated heat conduction mechanism 4Gene samples in sample pipe 5, gene samples carry out base in instrument internal under the action of amplification enzyme and by primer and gene actionIt is synthesized after cyclic amplification, and by various bases according to the sequence of sample gene, highly sensitive 13 light source of fluorescence detectorSample is excited by optical fiber 12 and releases fluorescence signal, and signal is collected into highly sensitive fluorescence detector 13 by optical fiber 12 and carries outImage checking forms image, and final image passes to control system 7 by data line, and by 7 screen of control system, we use Gao LingQuick camera replaces human eye to carry out the imaging results of observing samples, finally directly positive come the feminine gender of judgement sample by control system 7,The position of negative sample is imaged as fluorescence color at black, the position of positive sample, so as to be rapidly performed by next stepDiagnoses and treatment scheme.
Fuselage cover 1 of the invention, portable electric source mechanism 2, portable power 3, integrated heat conduction mechanism 4, examinationSample pipe 5, sample cell 6, control system 7, high-precision semiconductor temperature control member 8, conductor temperature controller 9, pedestal 10, lightThe components such as fibre 12, highly sensitive fluorescence detector 13, CCD camera 14, Heat Conduction Material 15 and frame 16 be universal standard part orComponent as known to those skilled in the art, structure and principle are all that this technology personnel can be learnt or be passed through by technical manualRoutine experiment method knows that gene-detecting apparatus volume compression has been arrived very small size by the present invention, is become portable instrumentDevice, can carry out real-time Amplification Analysis in field, carry out Sampling hold without carrying bulky liquid nitrogen container again.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industryPersonnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe thisThe principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changesChange and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and itsEquivalent thereof.