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CN109682941A - Utilize the method, apparatus and system of fish QRS interphase evaluation heavy metal in water - Google Patents

Utilize the method, apparatus and system of fish QRS interphase evaluation heavy metal in water
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Publication number
CN109682941A
CN109682941ACN201811572871.5ACN201811572871ACN109682941ACN 109682941 ACN109682941 ACN 109682941ACN 201811572871 ACN201811572871 ACN 201811572871ACN 109682941 ACN109682941 ACN 109682941A
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China
Prior art keywords
fish
heavy metal
water
electrocardiosignal
electrode
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CN201811572871.5A
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Chinese (zh)
Inventor
任宗明
任佰川
乔琳琳
任佰祥
刘跃丹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ji'nan Instrument Co Ltd
Shandong Normal University
South China Institute of Environmental Science of Ministry of Ecology and Environment
Original Assignee
Ji'nan Instrument Co Ltd
Shandong Normal University
South China Institute of Environmental Science of Ministry of Ecology and Environment
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Application filed by Ji'nan Instrument Co Ltd, Shandong Normal University, South China Institute of Environmental Science of Ministry of Ecology and EnvironmentfiledCriticalJi'nan Instrument Co Ltd
Priority to CN201811572871.5ApriorityCriticalpatent/CN109682941A/en
Priority to EP19756312.5Aprioritypatent/EP3646783A4/en
Priority to PCT/CN2019/072599prioritypatent/WO2020010836A1/en
Priority to CA3053603Aprioritypatent/CA3053603C/en
Publication of CN109682941ApublicationCriticalpatent/CN109682941A/en
Priority to US16/555,883prioritypatent/US10571448B2/en
Priority to ZA2019/05816Aprioritypatent/ZA201905816B/en
Pendinglegal-statusCriticalCurrent

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Abstract

Translated fromChinese

本公开公开了一种利用鱼类QRS间期评价水体重金属的方法、装置及系统,包括:获取鱼类接触待测水质前鱼体心电信号以及鱼类接触待测水质后若干特定时间点的鱼体心电信号;提取获取的各个鱼体心电信号QRS间期时长的心电指标;分析鱼类接触待测水质前以及鱼类接触待测水质后若干特定时间点的心电指标的变化,若变化超过预设阈值,则待测水体重金属含量超标,否则,待测水体重金属含量合格。

The present disclosure discloses a method, a device and a system for evaluating heavy metals in water by utilizing the QRS interval of fish, including: acquiring the ECG signal of the fish before the fish contacts the water quality to be tested, and the electrocardiographic signals of the fish at several specific time points after the fish contacts the water quality to be tested. ECG signal of fish body; extract the ECG index of QRS interval of each fish body ECG signal obtained; analyze the changes of ECG index at certain time points before fish contact with water quality to be tested and after fish contact with water quality to be tested , if the change exceeds the preset threshold, the heavy metal content of the water to be tested exceeds the standard; otherwise, the heavy metal content of the water to be tested is qualified.

Description

Utilize the method, apparatus and system of fish QRS interphase evaluation heavy metal in water
Technical field
The disclosure belongs to the technical field of water quality assessment, is related to a kind of utilizing fish QRS interphases evaluation heavy metal in waterMethod, apparatus and system.
Background technique
With the development of industrial or agricultural, the problem of heavy metal serious pollution ecology system, is had attracted more and more attention from people.WeightMetal is the noxious material for being easiest to accumulation, can be observed extensively in different biologic-organs.Heavy metal pollution is to biologyPhysiological function and normal mechanism generation seriously affect.Some developing regions in the world, in surface water, in surface waterCopper, lead, mercury can reach 73mg/L, 35mg/L, 1.14ug//L respectively, and there is also serious for the heavy metal contaminants such as manganese, chromiumIt is exceeded.
Currently, being used to the method for evaluating water quality has very much, it is generally adopted by physics, chemical method supervises water environmentIt surveys, although water quality rationally can be effectively monitored, its is with high costs, is not easy to implement to carry out and can only reflect partThe problems such as short term variations of the ecosystem, this brings certain difficulty to water quality monitoring work.In order to accurate measurements waterThe water quality condition of environment, more and more researchers evaluate using biology and monitor aquatic environment.
Summary of the invention
For the deficiencies in the prior art, a fish QRS interphase evaluation water body huge sum of money is utilized present disclose provides a kind ofThe method, apparatus and system of category, realize the simply acquisition fish electrocardiographicdata data that easily executes, and using fish electrocardiographicdata data asIndex is evaluated the heavy metal in water quality.
One or more other embodiments of the present disclosure provide a kind of method using fish QRS interphase evaluation heavy metal in water.
A method of heavy metal in water being evaluated using fish QRS interphase, this method comprises:
Obtain before fish contact measured water quality several specific times after fish body electrocardiosignal and fish contact measured water qualityThe fish body electrocardiosignal of point;
Extract the electrocardiographicdata data of each fish body electrocardiosignal QRS interphase duration obtained;
Analyze fish contact measured water quality before and fish contact measured water quality after several particular point in time electrocardiographicdata dataVariation, if variation is more than preset threshold, heavy metal in water content to be measured is exceeded, and otherwise, heavy metal in water content to be measured is closedLattice.
Further, in the method, obtain respectively before several fish contact measured water quality fish body electrocardiosignal andThe fish body electrocardiosignal of several particular point in time after contact measured water quality.
Further, this method further include: fish body electrocardiosignal is pre-processed, the pretreatment includes filtering and goesExcept interference is handled.
According to the other side of one or more other embodiments of the present disclosure, a kind of computer-readable storage medium is also providedMatter.
A kind of computer readable storage medium, wherein being stored with a plurality of instruction, described instruction is suitable for by terminal deviceReason device loads and executes a kind of method using fish QRS interphase evaluation heavy metal in water.
According to the other side of one or more other embodiments of the present disclosure, a kind of terminal device is also provided.
A kind of terminal device, using internet terminal equipment, including processor and computer readable storage medium, processorFor realizing each instruction;Computer readable storage medium is suitable for by processor load simultaneously for storing a plurality of instruction, described instructionExecute a kind of method using fish QRS interphase evaluation heavy metal in water.
According to the other side of one or more other embodiments of the present disclosure, also provides and a kind of commented using fish QRS interphaseThe system of valence heavy metal in water.
A kind of system using fish QRS interphase evaluation heavy metal in water, the system utilize fish QRS interphase based on a kind ofThe method for evaluating heavy metal in water is realized, comprising:
Fish electrocardio real-time acquisition device, infrared signal receiving apparatus and computing terminal equipment;
The fish electrocardio real-time acquisition device is installed in fish body by agreeing with the carrier of equipment for fish shape, and the carrier is realThe gravity and buoyancy equivalent state of existing carrier and fish electrocardio real-time acquisition device;
The fish electrocardio real-time acquisition device includes waterproof case, the miniature electrocardio letter of fixed placement in the waterproof caseNumber processing unit, storage device connected to it and battery, the miniature electrocardiogram signal processing device by conducting wire connection electrode,The electrode outlet of the waterproof case bottom end setting extraction electrode, electrode are pierced into acquisition original electrocardiographicdigital letter in fish body cardiocoelomNumber, and electrocardiosignal is handled by wire transmission to the miniature electrocardiogram signal processing device;The waterproof case sideThe through-hole for placing infrared signal emitter is arranged in face, and the infrared signal emitter transmitting terminal is by the waterproof caseThrough-hole is passed through in main body and is tightly connected with through-hole, and the infrared signal emitter is respectively and at battery and miniature electrocardiosignalDevice connection is managed, transmitting treated electrocardiosignal to infrared signal receiving apparatus engaged therewith completes fish heart real timeSignal acquisition;
The infrared signal receiving apparatus is installed on basin sidewall, for receiving treated electrocardiosignal, and will receiveElectrocardiosignal be sent to computing terminal equipment.
Further, the electrode of the system includes acquisition electrode and reference electrode, and the acquisition electrode is embedded to wait adoptIn the cardiocoelom of the fish of fish gathering body-centered electric signal, the reference electrode is embedded to the nephrodinic of the fish of fish body electrocardiosignal to be collectedNear hole, the acquisition electrode and the reference electrode are embedded to the intracorporal equal length of fish.
According to the other side of one or more other embodiments of the present disclosure, a kind of fish for evaluating heavy metal in water is also providedClass electrocardiographicdata data determines method, and this method is based on a kind of system using fish QRS interphase evaluation heavy metal in water.
It is a kind of evaluate heavy metal in water fish electrocardiographicdata data determine method, this method comprises:
The forward and backward fish body electrocardiosignal of solution of the fish contact containing specific heavy metal is obtained, and extracts a variety of electrocardios respectivelyIndex;
The correlation between environmental stress and the electrocardiographicdata data of each extraction, screening are analyzed according to pearson correlation analytic approachElectrocardiographicdata data for heavy metal in water evaluation;
According to linear regression between Akaike's Information Criterion and residual sum of squares (RSS) assay environmental stress and each electrocardiographicdata dataModel obtains the electrocardiographicdata data evaluated eventually for heavy metal in water.
According to the other side of one or more other embodiments of the present disclosure, a kind of computer-readable storage medium is also providedMatter.
A kind of computer readable storage medium, wherein being stored with a plurality of instruction, described instruction is suitable for by terminal deviceReason device load and a kind of fish electrocardiographicdata data of evaluation heavy metal in water described in executing determines method.
According to the other side of one or more other embodiments of the present disclosure, a kind of terminal device is also provided.
A kind of terminal device, using internet terminal equipment, including processor and computer readable storage medium, processorFor realizing each instruction;Computer readable storage medium is suitable for by processor load simultaneously for storing a plurality of instruction, described instructionIt executes a kind of fish electrocardiographicdata data for evaluating heavy metal in water and determines method.
The disclosure the utility model has the advantages that
A kind of method, apparatus and system using fish QRS interphase evaluation heavy metal in water, proposes described in the disclosureA kind of effective solution method for evaluating heavy metal in water as index using electrocardiographicdata data, realize simply easily execute adoptCollect fish electrocardiographicdata data, and using fish electrocardiographicdata data as index evaluating to the heavy metal in water quality;Especially byHeavy metal in the electrocardiographicdata data evaluating water quality of fish QRS interphase duration.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's showsMeaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is the side that one of disclosure one or more embodiment evaluates heavy metal in water using fish QRS interphaseMethod flow chart;
Fig. 2 is the fish body electrocardiogram of the acquisition in disclosure one or more embodiment;
Fig. 3 is that one of disclosure one or more embodiment is using what fish QRS interphase evaluated heavy metal in waterSystem structural schematic diagram;
Wherein, 1 is waterproof case, and 2 be battery, and 3 be infrared signal emitter, and 4 be miniature electrocardiogram signal processing device,5 be conducting wire, and 6 be electrode, and 7 be carrier, and 8 be fish body, and 9 be sink, and 10 be infrared signal receiving apparatus, and 11 be computer.
Specific embodiment:
Below in conjunction with the attached drawing in the embodiment of the present disclosure, the technical solution in the embodiment of the present disclosure is carried out clear, completeSite preparation description, it is clear that described embodiment is only disclosure a part of the embodiment, instead of all the embodiments.It is based onEmbodiment in the disclosure, it is obtained by those of ordinary skill in the art without making creative efforts every otherEmbodiment belongs to the range of disclosure protection.
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless anotherIt indicates, all technical and scientific terms that the present embodiment uses have and the application person of an ordinary skill in the technical fieldNormally understood identical meanings.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted rootAccording to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singularAlso it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packetInclude " when, indicate existing characteristics, step, operation, device, component and/or their combination.
It should be noted that flowcharts and block diagrams in the drawings show according to various embodiments of the present disclosure method andThe architecture, function and operation in the cards of system.It should be noted that each box in flowchart or block diagram can representA part of one module, program segment or code, a part of the module, program segment or code may include one or moreA executable instruction for realizing the logic function of defined in each embodiment.It should also be noted that some alternatelyRealization in, function marked in the box can also occur according to the sequence that is marked in attached drawing is different from.For example, two connectThe box even indicated can actually be basically executed in parallel or they can also be executed in a reverse order sometimes,This depends on related function.It should also be noted that each box and flow chart in flowchart and or block diagramAnd/or the combination of the box in block diagram, the dedicated hardware based system that functions or operations as defined in executing can be used are comeIt realizes, or the combination of specialized hardware and computer instruction can be used to realize.
In the absence of conflict, the features in the embodiments and the embodiments of the present application can be combined with each other below with reference toAttached drawing and embodiment are described further the disclosure.
One or more other embodiments of the present disclosure provide a kind of method using fish QRS interphase evaluation heavy metal in water.
As shown in Figure 1, a kind of method using fish QRS interphase evaluation heavy metal in water, this method comprises:
Obtain before fish contact measured water quality several specific times after fish body electrocardiosignal and fish contact measured water qualityThe fish body electrocardiosignal of point;
Extract the electrocardiographicdata data of each fish body electrocardiosignal QRS interphase duration obtained;
Analyze fish contact measured water quality before and fish contact measured water quality after several particular point in time electrocardiographicdata dataVariation, if variation is more than preset threshold, heavy metal in water content to be measured is exceeded, and otherwise, heavy metal in water content to be measured is closedLattice.
Further, in the method, obtain respectively before several fish contact measured water quality fish body electrocardiosignal andThe fish body electrocardiosignal of several particular point in time after contact measured water quality.
Further, this method further include: fish body electrocardiosignal is pre-processed, the pretreatment includes filtering and goesExcept interference is handled.
According to the other side of one or more other embodiments of the present disclosure, a kind of computer-readable storage medium is also providedMatter.
A kind of computer readable storage medium, wherein being stored with a plurality of instruction, described instruction is suitable for by terminal deviceReason device loads and executes a kind of method using fish QRS interphase evaluation heavy metal in water.
According to the other side of one or more other embodiments of the present disclosure, a kind of terminal device is also provided.
A kind of terminal device, using internet terminal equipment, including processor and computer readable storage medium, processorFor realizing each instruction;Computer readable storage medium is suitable for by processor load simultaneously for storing a plurality of instruction, described instructionExecute a kind of method using fish QRS interphase evaluation heavy metal in water.
According to the other side of one or more other embodiments of the present disclosure, also provides and a kind of commented using fish QRS interphaseThe system of valence heavy metal in water.
A kind of system using fish QRS interphase evaluation heavy metal in water, the system utilize fish QRS interphase based on a kind ofThe method for evaluating heavy metal in water is realized, comprising: fish electrocardio real-time acquisition device, infrared signal receiving apparatus and computing terminalEquipment;
The fish electrocardio real-time acquisition device is installed in fish body by agreeing with the carrier of equipment for fish shape, and the carrier is realThe gravity and buoyancy equivalent state of existing carrier and fish electrocardio real-time acquisition device;
The fish electrocardio real-time acquisition device includes waterproof case, the miniature electrocardio letter of fixed placement in the waterproof caseNumber processing unit, storage device connected to it and battery, the miniature electrocardiogram signal processing device by conducting wire connection electrode,The electrode outlet of the waterproof case bottom end setting extraction electrode, electrode are pierced into acquisition original electrocardiographicdigital letter in fish body cardiocoelomNumber, and electrocardiosignal is handled by wire transmission to the miniature electrocardiogram signal processing device;The waterproof case sideThe through-hole for placing infrared signal emitter is arranged in face, and the infrared signal emitter transmitting terminal is by the waterproof caseThrough-hole is passed through in main body and is tightly connected with through-hole, and the infrared signal emitter is respectively and at battery and miniature electrocardiosignalDevice connection is managed, transmitting treated electrocardiosignal to infrared signal receiving apparatus engaged therewith completes fish heart real timeSignal acquisition;
The infrared signal receiving apparatus is installed on basin sidewall, for receiving treated electrocardiosignal, and will receiveElectrocardiosignal be sent to computing terminal equipment.
Further, the electrode of the system includes acquisition electrode and reference electrode, and the acquisition electrode is embedded to wait adoptIn the cardiocoelom of the fish of fish gathering body-centered electric signal, the reference electrode is embedded to the nephrodinic of the fish of fish body electrocardiosignal to be collectedNear hole, the acquisition electrode and the reference electrode are embedded to the intracorporal equal length of fish.
According to the other side of one or more other embodiments of the present disclosure, a kind of fish for evaluating heavy metal in water is also providedClass electrocardiographicdata data determines method, and this method is based on a kind of system using fish QRS interphase evaluation heavy metal in water.
It is a kind of evaluate heavy metal in water fish electrocardiographicdata data determine method, this method comprises:
The forward and backward fish body electrocardiosignal of solution of the fish contact containing specific heavy metal is obtained, and extracts a variety of electrocardios respectivelyIndex;
The correlation between environmental stress and the electrocardiographicdata data of each extraction, screening are analyzed according to pearson correlation analytic approachElectrocardiographicdata data for heavy metal in water evaluation;
According to linear regression between Akaike's Information Criterion and residual sum of squares (RSS) assay environmental stress and each electrocardiographicdata dataModel obtains the electrocardiographicdata data evaluated eventually for heavy metal in water.
Further, in the method, several fish are obtained respectively in the different heavy metals of different exposure concentrations notWith the fish body electrocardiosignal of particular point in time.
Further, in the method, the electrocardiographicdata data of extraction includes: P wave-amplitude, Q wave-amplitude, R wave-amplitude, the vibration of S waveWidth, T wave-amplitude and P-R interphase duration, QRS interphase duration, S-T interphase duration and Q-T interphase duration.
Further, in the method, also fish body electrocardiosignal is pre-processed, the pretreatment includes filtering and goesExcept interference is handled.
Further, in the method, before to fish body ECG's data compression, also by the fish body electrocardiosignal of acquisitionIt is handled by fish electrocardio online acquisition with analysis system.
It further, in the method, further include analyzing environmental stress according to pearson correlation analytic approach and each mentioningBefore the correlation between electrocardiographicdata data taken, determine specially heavy metal concentration variation and the time change to fish body electrocardiographicdata dataEffect tendency.
Further, to determine that the QT interphase in fish electrocardiographicdata data and the heavy metal stress in water quality have by this method brightAobvious correlation evaluates the heavy metal in water quality by the QT interphase in fish electrocardiographicdata data.
This method mainly carries out electrocardiogram acquisition by multi-channel Physiological Signal Acquiring System, then believes collected electrocardioNumber carrying out preliminary treatment obtains data, then handles further evaluating water quality by software.
This method is acquired the electrocardiosignal of fish by a kind of cardiac electrical method and system of online acquisition, the acquisition systemSystem mainly includes fish electrocardio real-time acquisition device, infrared signal receiving apparatus and computing terminal equipment;The fish electrocardio is realWhen acquisition device be installed in fish body by agreeing with the carrier of equipment for fish shape, the carrier realizes that carrier is adopted in real time with fish electrocardioThe gravity and buoyancy equivalent state of acquisition means;The fish electrocardio real-time acquisition device includes waterproof case, the waterproof caseThe miniature electrocardiogram signal processing device of interior fixed placement, storage device connected to it and battery, the miniature ECG's data compressionDevice is pierced into fish body and is enclosed by conducting wire connection electrode, the electrode outlet of the waterproof case bottom end setting extraction electrode, electrodeOriginal electro-cardiologic signals are acquired in the chambers of the heart, and electrocardiosignal is carried out by wire transmission to the miniature electrocardiogram signal processing deviceProcessing;The through-hole for placing infrared signal emitter, the infrared signal emitter is arranged in the waterproof case sideTransmitting terminal by the waterproof case main body pass through through-hole and with through-hole be tightly connected, the infrared signal emitter respectively withBattery is connected with miniature electrocardiogram signal processing device, and treated that electrocardiosignal to IR signal reception engaged therewith fills for transmittingIt sets, completes fish heart real time signal acquisition;The infrared signal receiving apparatus is installed on basin sidewall, after reception processingElectrocardiosignal, and received electrocardiosignal is sent to computing terminal equipment.;The acquisition method can be relatively stable acquisitionElectrocardiosignal can also try one's best and reduce extraneous interference to the influence of signal acquisition bring;Again by collectedElectrocardiogram is filtered and removes interference processing, obtains relatively sharp electrocardiogram;Then electrocardiogram is carried out by system softwareAnalysis, the data after analysis are handled, then treated data Matlab software and SPSS software are analyzed, rootCardiac electrical variation is judged according to data difference.
In each parameter of electrocardio, (Pearson correlation) Lai Jinhang is analyzed using pearson correlation in SPSSCorrelation analysis between environmental stress (E) and each EGC parameter is with the index (skin of the EGC parameter in specified Water Quality EvaluationThe absolute value of Ademilson correlation coefficient r is closer to 1, and correlation is better, and p value is smaller, and correlation is more significant, and p < 0.05 hasConspicuousness, p < 0.01 have extremely significant property, and p < 0.001 has extremely extremely significant property);Wherein, environmental pressure E and pollutant is denseIt spends related with the time.Then, further pass through Akaike's Information Criterion (AIC) and be based on residual sum of squares (RSS) (SSE), environment is answeredLinear regression model (LRM) between power (E) and each electrocardiogram parameters has carried out assay (wherein, the residual error in linear regression model (LRM)Quadratic sum SSE is smaller, and AIC value is smaller, and the model-fitting degree is better).
Biological monitoring can be direct according to the physiology of organism and behavior and continues to detect various pollutants and poisonous effect.Meanwhile biological water quality monitoring cost and maintenance it is all lower.Biological monitoring can combining environmental condition at any time.Biological monitoringThere are many kinds of methods, but seldom realizes using EGC parameter as evaluation index.
The method that water environment is assessed are as follows: by fish electrocardio online acquisition and analysis system, acquire fish respectively and existElectrocardiosignal before and after contact measured water, and extract P, Q, R, S and T wave-amplitude, P-R interphase, QRS interphase, S-T interphase and Q-9 indexs of T interphase, the variation for analyzing electrocardiographicdata data of the fish before and after contact measured water prove water to be measured to fish if changingElectrocardio produces adverse effect;If unchanged, prove that water to be measured is little without influencing or influencing on fish electrocardio.
The correlation analysis of QRS interphase and environmental pressure (E) based on linear regression show P wave and R wave respectively with copper, lead,Mercury, manganese, chromium environmental pressure (E) there are significant related these to show that the duration of QRS interphase and environmental pressure (E) are related.
Then, further pass through Akaike's Information Criterion (AIC) and be based on residual sum of squares (RSS) (SSE), to environmental stress (E)Linear regression model (LRM) between each electrocardiogram parameters has carried out assay.Analysis shows that, established according to data with QRS interphaseLinear regression model (LRM) in, the value of R2 is maximum, and residual sum of squares (RSS) (SSE) is minimum, and AIC value is minimum.In such a case, it is possible toIt draws a conclusion, QRS interphase can use an index in copper, lead, mercury, manganese, chromium stress assessment.In each parameter of electrocardio, pass throughOrganising map (SOM) show include heavy metal copper, lead, mercury, manganese, chromium stress and all EGC parameters classification data mode in,QRS is extremely similar to environmental pressure.This shows that the duration of QRS interphase is related with environmental pressure E.Between QRS based on linear regressionThe correlation analysis of phase and environmental pressure shows correlation coefficient r=0.729 and related significance p < 0.01, and there are extremely significant relationships.In such a case, it is possible to draw a conclusion, QRS interphase may be used as one in the assessment of the heavy metal stress such as copper, lead, mercury, manganese, chromiumA index.
This shows that the duration of QRS complex pressure E related with environment is direct.The rule of ECG parameter in heavy metal pollutionModel and assessment, which show that there are one, has significant correlation, r value highest, all ECG spies between QRS complex and heavy metal stressMinimum p in sign.In such a case, it is possible to draw a conclusion, QRS interphase can be assessed with heavy metal stress in index.
According to one or more other embodiments of the present disclosure the specific technical proposal is:
Fancy carp is exposed in copper-bath first, copper sulphate is 10.3mg/L for the 48h half lethal concentration of fancy carp,It is denoted as 1 toxic unit (1TU), fancy carp is exposed in the copper sulphate of various concentration and is dealt with, exposure concentrations are respectively 1.3mg/L, 5.15mg/L (0.1TU, 0.5TU) and 1mg/L.Carry out the acquisition of fancy carp electrocardiosignal, ecg signal acquiring memory and saddleShape carrier is fixed on fish body back, so that fish is carried the device and is in normal condition in water and freely moves about, and no weight bearing is existingAs then carrying out 3 groups of parallel laboratory tests, the last processing that we take the electrocardiosignal result of three groups of parallel laboratory tests to carry out electrocardiographicdata dataAnalysis.
Identical as the technical solution for doing copper sulphate, mercury, cadmium, manganese are also the same experimental procedure, wherein mercuric chloride solution pairIt is 0.13mg/L in the 48h half lethal concentration of fancy carp, is denoted as 1 toxic unit (1TU), fancy carp is exposed to the chlorination of various concentrationIt is dealt in mercury solution, exposure concentrations are respectively 0.013mg/L, 0.065mg/L (0.1TU, 0.5TU) and 0.05ug/L.It carries outThe acquisition of fancy carp electrocardiosignal, ecg signal acquiring memory and saddle carrier are fixed on fish body back, and fish is enable to carry thisDevice is in normal condition in water and freely moves about, then no weight bearing phenomenon carries out 3 groups of parallel laboratory tests, and last we take threeThe electrocardiosignal result of group parallel laboratory test carries out the processing analysis of electrocardiographicdata data.
Cadmium chloride solution is 23.6mg/L for the 48h half lethal concentration of fancy carp, 1 toxic unit (1TU) is denoted as, by fancy carpIt being exposed in the hexavalent chromium solution of various concentration and deals with, exposure concentrations are respectively 2.36mg/L, 11.8mg/L (0.1TU,0.5TU) and 0.05mg/L.The acquisition of fancy carp electrocardiosignal is carried out, ecg signal acquiring memory and saddle carrier are fixed on fishBody back enables fish to carry the device and be in normal condition in water and freely moves about, then no weight bearing phenomenon carries out 3 groupsParallel laboratory test, the last processing analysis that we take the electrocardiosignal result of three groups of parallel laboratory tests to carry out electrocardiographicdata data.
Manganese solution is 2.75mg/L for the 48h half lethal concentration of fancy carp, is denoted as 1 toxic unit (1TU), by fancy carp exposureDealt in the manganese solution of various concentration, exposure concentrations are respectively 0.275mg/L, 1.375mg/L (0.1TU, 0.5TU) and0.1mg/L.The acquisition of fancy carp electrocardiosignal is carried out, ecg signal acquiring memory and saddle carrier are fixed on fish body back, makeFish, which can carry the device and be in normal condition in water, freely to move about, then no weight bearing phenomenon carries out 3 groups of parallel laboratory tests,The processing that finally we take the electrocardiosignal result of three groups of parallel laboratory tests to carry out electrocardiographicdata data is analyzed.
It is that measurement is acquired by a kind of cardiac electrical system of online acquisition for the electrocardiogram acquisition of fancy carp.The acquisition systemSystem mainly includes ecg signal acquiring memory, signal projector, signal receiver, electrode, conducting wire, battery, waterproof case, saddleShape carrier;Host etc., the acquisition electrocardiosignal which can be relatively stable.
Wherein silvery acquisition electrode is embedded in the cardiocoelom of fancy carp by we, and the cloacal aperture of reference electrode embedment fancy carp is attachedClosely, the electrode length for being embedded to fish body is equal, about 2-4mm.Ecg signal acquiring memory and saddle carrier are fixed on fish body backPortion enables fish to carry the ecg signal acquiring and be in normal condition in water with emitter and freely moves about, and no weight bearing is existingAs carrying out acquisition monitoring in real time to its electrocardio under the normal condition of fish., collected electrocardiosignal is emitted to by infrared rayElectrocardiosignal receiver is received;Host can analyze fish body ecg information, pass through fish electrocardio online acquisition and analysis systemCollected electrocardiosignal is output to text document;Host extracts P wave-amplitude, Q wave from the fish body electrocardiosignal receivedAmplitude, R wave-amplitude, S wave-amplitude, T wave-amplitude and P-R interphase duration, QRS interphase duration, S-T interphase duration and Q-T interphase9 electrocardiographicdata datas of duration.The variation for analyzing electrocardiographicdata data of the fish before and after contact measured water proves water to be measured if changingAdverse effect is produced to fish electrocardio;If unchanged, prove that water to be measured is little without influencing or influencing on fish electrocardio.
By collected electrocardiogram being filtered and being removed interference processing, relatively sharp electrocardiogram, such as Fig. 2 are obtainedIt is shown;Then ecg analysis is carried out by Matlab and SPSS, the data after analysis is handled.Analyze fish contact toThe variation for the electrocardiographicdata data surveyed before and after water proves that water to be measured produces adverse effect to fish electrocardio if changing;If without changeChange, then proves that water to be measured is little without influencing or influencing on fish electrocardio.In each parameter of electrocardio, Pearson's phase is used in SPSSClose analysis (Pearson correlation) Lai Jinhang environmental stress (E) and each EGC parameter between correlation analysis withEGC parameter in specified Water Quality Evaluation index (for the absolute value of Pearson correlation coefficients r closer to 1, correlation is better, andP value is smaller, and correlation is more significant, and p < 0.05 has conspicuousness, and p < 0.01 has extremely significant property, and p < 0.001 has pole extremely aobviousWork property);Wherein, environmental pressure E is related with the concentration of pollutant and time.Then, further pass through Akaike's Information Criterion(AIC) and it is based on residual sum of squares (RSS) (SSE), the linear regression model (LRM) between environmental stress (E) and each electrocardiogram parameters is carried out(the residual sum of squares (RSS) SSE wherein, in linear regression model (LRM) is smaller, and AIC value is smaller, which gets over for assayIt is good).
The correlation analysis of QRS interphase and environmental pressure (E) based on linear regression shows and QRS interphase and environmental pressure(E) there is extremely significant correlation, this shows that the environmental pressure (E) that the duration of QRS interphase is coerced with heavy metal contaminants is related.SoAfterwards, further pass through Akaike's Information Criterion (AIC) and be based on residual sum of squares (RSS) (SSE), to environmental stress (E) and each electrocardiogramLinear regression model (LRM) between parameter has carried out assay.According to data analysis shows that, with QRS interphase establish linear regressionIn model, R2Value it is maximum, residual sum of squares (RSS) (SSE) is minimum, and AIC value is minimum.Most of all, in all EGC parametersIn classification data mode, QRS interphase is extremely similar to environmental pressure.This shows that the duration of QRS interphase is related with environmental pressure E.In such a case, it is possible to draw a conclusion, QRS interphase may be used as an index in Cd stress assessment.
According to one or more other embodiments of the present disclosure, QRS interphase and heavy metal contaminants stress have apparent correlation.Heavy metal pollution of water body object can be monitored by research observation and analysis QRS interphase.
According to the other side of one or more other embodiments of the present disclosure, a kind of computer-readable storage medium is also providedMatter.
A kind of computer readable storage medium, wherein being stored with a plurality of instruction, described instruction is suitable for by terminal deviceReason device load and a kind of fish electrocardiographicdata data of evaluation heavy metal in water described in executing determines method.
According to the other side of one or more other embodiments of the present disclosure, a kind of terminal device is also provided.
A kind of terminal device, using internet terminal equipment, including processor and computer readable storage medium, processorFor realizing each instruction;Computer readable storage medium is suitable for by processor load simultaneously for storing a plurality of instruction, described instructionIt executes a kind of fish electrocardiographicdata data for evaluating heavy metal in water and determines method.
These computer executable instructions execute the equipment according to each reality in the disclosureApply method or process described in example.
In the present embodiment, computer program product may include computer readable storage medium, containing for holdingThe computer-readable program instructions of row various aspects of the disclosure.Computer readable storage medium, which can be, can keep and storeBy the tangible device for the instruction that instruction execution equipment uses.Computer readable storage medium for example can be-- but it is unlimitedIn-- storage device electric, magnetic storage apparatus, light storage device, electric magnetic storage apparatus, semiconductor memory apparatus or above-mentionedAny appropriate combination.The more specific example (non exhaustive list) of computer readable storage medium includes: portable computingMachine disk, hard disk, random access memory (RAM), read-only memory (ROM), erasable programmable read only memory (EPROM orFlash memory), static random access memory (SRAM), Portable compressed disk read-only memory (CD-ROM), digital versatile disc(DVD), memory stick, floppy disk, mechanical coding equipment, the punch card for being for example stored thereon with instruction or groove internal projection structure, withAnd above-mentioned any appropriate combination.Computer readable storage medium used herein above is not interpreted instantaneous signal itself,The electromagnetic wave of such as radio wave or other Free propagations, the electromagnetic wave propagated by waveguide or other transmission mediums (for example,Pass through the light pulse of fiber optic cables) or pass through electric wire transmit electric signal.
Computer-readable program instructions described herein can be downloaded to from computer readable storage medium it is each calculate/Processing equipment, or outer computer or outer is downloaded to by network, such as internet, local area network, wide area network and/or wireless networkPortion stores equipment.Network may include copper transmission cable, optical fiber transmission, wireless transmission, router, firewall, interchanger, gatewayComputer and/or Edge Server.Adapter or network interface in each calculating/processing equipment are received from network to be countedCalculation machine readable program instructions, and the computer-readable program instructions are forwarded, for the meter being stored in each calculating/processing equipmentIn calculation machine readable storage medium storing program for executing.
Computer program instructions for executing present disclosure operation can be assembly instruction, instruction set architecture (ISA)Instruction, machine instruction, machine-dependent instructions, microcode, firmware instructions, condition setup data or with one or more programmingsThe source code or object code that any combination of language is write, the programming language include the programming language-of object-oriented such asC++ etc., and conventional procedural programming languages-such as " C " language or similar programming language.Computer-readable program refers toOrder can be executed fully on the user computer, partly be executed on the user computer, as an independent software packageExecute, part on the user computer part on the remote computer execute or completely on a remote computer or serverIt executes.In situations involving remote computers, remote computer can include local area network by the network-of any kind(LAN) or wide area network (WAN)-is connected to subscriber computer, or, it may be connected to outer computer (such as utilize internetService provider is connected by internet).In some embodiments, by being believed using the state of computer-readable program instructionsBreath comes personalized customization electronic circuit, such as programmable logic circuit, field programmable gate array (FPGA) or programmable logicArray (PLA), the electronic circuit can execute computer-readable program instructions, to realize the various aspects of present disclosure.
The disclosure the utility model has the advantages that
A kind of method, apparatus and system using fish QRS interphase evaluation heavy metal in water, proposes described in the disclosureUsing electrocardiographicdata data as index come a kind of effective solution method of evaluating water quality, realize the simply acquisition fish that easily executeElectrocardiographicdata data, and using fish electrocardiographicdata data as index evaluating to the heavy metal in water quality;
By heavy metal different in the different electrocardiographicdata data evaluating water quality of fish, filter out using in fish electrocardiographicdata dataQT interphase monitor heavy metal in water.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this fieldFor art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repairChange, equivalent replacement, improvement etc., should be included within the scope of protection of this application.Therefore, the disclosure is not intended to be limited to thisThese embodiments shown in text, and it is to fit to the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

The fish electrocardio real-time acquisition device includes waterproof case, in the waterproof case at the miniature electrocardiosignal of fixed placementDevice, storage device connected to it and battery are managed, the miniature electrocardiogram signal processing device is described by conducting wire connection electrodeThe electrode outlet of extraction electrode is arranged in waterproof case bottom end, and electrode, which is pierced into fish body cardiocoelom, acquires original electro-cardiologic signals, andElectrocardiosignal is handled by wire transmission to the miniature electrocardiogram signal processing device;The waterproof case side settingFor placing the through-hole of infrared signal emitter, the infrared signal emitter transmitting terminal is by the waterproof case main bodyAcross through-hole and with through-hole be tightly connected, the infrared signal emitter respectively with battery and miniature electrocardiogram signal processing deviceConnection, transmitting treated electrocardiosignal to infrared signal receiving apparatus engaged therewith are completed fish heart real time signal and are adoptedCollection;
CN201811572871.5A2018-07-112018-12-21Utilize the method, apparatus and system of fish QRS interphase evaluation heavy metal in waterPendingCN109682941A (en)

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CN201811572871.5ACN109682941A (en)2018-12-212018-12-21Utilize the method, apparatus and system of fish QRS interphase evaluation heavy metal in water
EP19756312.5AEP3646783A4 (en)2018-07-112019-01-22 COMPLETE ASSESSMENT PROCESS AND SYSTEM BASED ON A FISH ELECTROCARDIOGRAM FOR POLLUTION OF ORGANIC MATTER AND WATER TO HEAVY METALS
PCT/CN2019/072599WO2020010836A1 (en)2018-07-112019-01-22Fish electrocardiogram based comprehensive evaluation method and system for organic matter and heavy-metal water pollution
CA3053603ACA3053603C (en)2018-07-112019-01-22Method and system for comprehensive evaluation of organic compound and heavy metal pollution in water based on fish electrocardio
US16/555,883US10571448B2 (en)2018-07-112019-08-29Method and system for comprehensive evaluation of organic compound and heavy metal pollution in water based on fish electrocardio
ZA2019/05816AZA201905816B (en)2018-07-112019-09-03Method and system for comprehensive evaluation of organic compound and heavy metal pollution in water based on fish electrocardio

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