CN109652288B - Automatic genotyping equipment and method - Google Patents

Abstract

The invention discloses automatic genotyping equipment and a method, and relates to the field of genotyping, wherein the genotyping equipment comprises a sample moving device, a water bath device, a data acquisition device, a sample moving PLC module, a water bath PLC module, an acquisition PLC module and a data analysis module; the genotyping method comprises: setting a pipetting program; preparing a reagent; adding a DNA sample into the pore plate; the reaction plate is placed in the pipetting position; selecting a pipetting scheme, carrying out a pipetting program, and packaging the reaction plate by an automatic heat sealing machine; moving the reaction plate after heat sealing into a water bath tank; collecting data of the reaction plate after water bath by using a data collecting device; reading the sample typing result by using a data analysis module; the device can meet the use requirements of large sample amount, few experiment operators and high requirements, save a large amount of manpower by using the device, and improve the accuracy of experiment results.

Description

Translated fromChinese

一种自动化基因分型设备及方法A kind of automatic genotyping equipment and method

技术领域technical field

本发明涉及基因分型领域，尤其涉及一种自动化基因分型设备及方法。The invention relates to the field of genotyping, in particular to an automatic genotyping device and method.

背景技术Background technique

基因分型技术以其出色的生物识别分辨能力和近乎痕量的检测灵敏度，已广泛应用于动植物物种鉴定、病原菌分子分型、人身份识别(法医学鉴定、亲子鉴定等)、疾病相关基因型分析等领域。但现有的基因分型系统存在装置结构简单、功能单一、精度和稳定性不高、单次检测成本较高、通量较低或自动化程度低，并且在工作过程中无法做到超微量移液，在移液过程中可能与试剂发生接触造成污染，影响最终实验结果等问题。Genotyping technology has been widely used in the identification of animal and plant species, molecular typing of pathogenic bacteria, human identification (forensic identification, paternity testing, etc.) analysis, etc. However, the existing genotyping systems have the disadvantages of simple device structure, single function, low accuracy and stability, high single detection cost, low throughput or low degree of automation, and cannot achieve ultra-micro transfer in the working process. During the pipetting process, it may come into contact with the reagents, causing contamination and affecting the final experimental results.

发明内容SUMMARY OF THE INVENTION

本发明的目的就在于为了解决上述问题设计了一种自动化基因分型设备及方法。The purpose of the present invention is to design an automatic genotyping device and method in order to solve the above problems.

本发明通过以下技术方案来实现上述目的：The present invention realizes above-mentioned purpose through following technical scheme:

一种自动化基因分型设备，包括用于移动试剂或DNA样品的移样装置、用于扩增PCR的水浴装置、采集各项数据的数据采集装置、移样PLC模块、水浴PLC模块、采集PLC模块和数据分析模块；An automated genotyping device, comprising a sample transfer device for moving reagents or DNA samples, a water bath device for amplifying PCR, a data acquisition device for collecting various data, a sample transfer PLC module, a water bath PLC module, and a collection PLC modules and data analysis modules;

移样装置包括用于移动试剂的非接触式移液装置、用于移动DNA样品的接触式移液装置、装有试剂的试剂槽、装有DNA样品的孔板和热封装置，非接触式移液装置包括反应板，非接触式移液装置用于移动试剂槽内的试剂或孔板内的DNA样品，热封装置用于热封装有试剂和DNA样品的反应板，非接触式移液装置与接触式移液装置固定连接，移样PLC模块的信号端分别与热封装置、非接触式移液装置和接触式移液装置的信号端连接；The pipetting device includes a non-contact pipetting device for moving reagents, a contact pipetting device for moving DNA samples, a reagent tank containing reagents, a well plate containing DNA samples, and a heat-sealing device, non-contact type The pipetting device includes the reaction plate, the non-contact pipetting device is used to move the reagent in the reagent tank or the DNA sample in the well plate, and the heat sealing device is used to heat the reaction plate with the reagent and DNA sample, and the non-contact pipetting The device is fixedly connected with the contact pipetting device, and the signal end of the sample pipetting PLC module is respectively connected with the signal ends of the heat sealing device, the non-contact pipetting device and the contact pipetting device;

水浴装置包括水浴箱、用于移动热封后反应板的第一移动装置、控制水浴箱水位的液控装置和用于控制水浴箱内温度的温控装置，热封后的反应板置于第一移动装置的移动端，第一移动装置设置在水浴箱的上方，将反应板在水浴箱内移进移出，水浴PLC模块的信号端分别与温控装置、液控装置和第一移动装置的信号端连接；The water bath device includes a water bath box, a first moving device for moving the reaction plate after heat sealing, a liquid control device for controlling the water level of the water bath box, and a temperature control device for controlling the temperature in the water bath box. A mobile end of the mobile device, the first mobile device is arranged above the water bath box, the reaction plate is moved in and out of the water bath box, the signal end of the water bath PLC module is respectively connected with the temperature control device, the hydraulic control device and the first mobile device. signal terminal connection;

数据采集装置用于移动水浴后反应板的第二移动装置、多个光源发射装置、用于采集反应板在不同光源下的信息采集装置、用于移动的信息采集装置的第三移动装置，水浴后的反应板置于第二移动装置的移动端，第二移动装置将反应板移动到不同的光信息采集位，信息采集装置与第三移动装置的移动端固定连接，第三移动装置将信息采集装置移动到不同的光信息采集位，信息采集装置对反应板在不同光源下的光信息进行采集，多个光源发射装置位于同一平面上，第三移动装置设置在多个光源发射装置的下方，第二移动装置设置在第三移动装置的下方，采集PLC模块的信号端分别与第二移动装置、第三移动装置、信息采集装置和光源发射装置的信号端连接；The data acquisition device is used for the second moving device of the reaction plate after moving the water bath, a plurality of light source emission devices, the information collecting device for collecting the reaction plate under different light sources, the third moving device for the moving information collecting device, the water bath The rear reaction plate is placed on the mobile end of the second mobile device, the second mobile device moves the reaction plate to different optical information collection positions, the information collection device is fixedly connected with the mobile end of the third mobile device, and the third mobile device collects the information. The collection device moves to different light information collection positions, the information collection device collects the light information of the reaction plate under different light sources, the multiple light source emission devices are located on the same plane, and the third moving device is arranged below the multiple light source emission devices , the second mobile device is arranged below the third mobile device, and the signal terminals of the acquisition PLC module are respectively connected with the signal terminals of the second mobile device, the third mobile device, the information acquisition device and the light source emission device;

数据分析模块的数据信号端与信息采集装置的数据信号端连接。The data signal end of the data analysis module is connected with the data signal end of the information acquisition device.

进一步地，非接触式移液装置还包括移液模块、平移组件和控制模块，平移组件固定设置在移液位置的上方，反应板置于移液位置上，移液模块的移液端与平移组件的移动端固定连接，控制组件与移液模块连接；移液模块包括移液针组、溶液罐、吸液泵、气压泵和压力控制容器，溶液罐分别与移液针组、吸液泵和压力控制容器连通，压力控制容器与气压泵连通；控制模块包括高频电磁阀、第一电磁阀、第二电磁阀、第三电磁阀和比例阀，高频电磁阀设置在移液针组与溶液罐之间的液路上，第一电磁阀设置在溶液罐与压力控制容器之间的液路上，第二电磁阀设置在吸液泵与溶液罐之间的液路上，第三电磁阀设置在吸液泵与压力控制容器之间的液路上，比例阀设置在压力控制容器与气压泵之间的气路上。Further, the non-contact pipetting device also includes a pipetting module, a translation component and a control module, the translation component is fixedly arranged above the pipetting position, the reaction plate is placed on the pipetting position, and the pipetting end of the pipetting module is connected with the translation. The mobile end of the component is fixedly connected, and the control component is connected with the pipetting module; the pipetting module includes a pipetting needle group, a solution tank, a suction pump, an air pressure pump and a pressure control container, and the solution tank is respectively connected with the pipetting needle group and the liquid suction pump. It is communicated with the pressure control container, and the pressure control container is communicated with the air pressure pump; the control module includes a high-frequency solenoid valve, a first solenoid valve, a second solenoid valve, a third solenoid valve and a proportional valve, and the high-frequency solenoid valve is arranged in the pipetting needle group. On the liquid path between the solution tank and the solution tank, the first solenoid valve is set on the liquid path between the solution tank and the pressure control container, the second solenoid valve is set on the liquid path between the suction pump and the solution tank, and the third solenoid valve is set On the liquid path between the suction pump and the pressure control container, a proportional valve is arranged on the air path between the pressure control container and the air pressure pump.

进一步地，接触式移液装置包括塑料针组和伺服电机，伺服电机通过连接螺丝与塑料针组的活塞固定连接，塑料针组的针头与移液针组的针头相邻安装。Further, the contact type pipetting device includes a plastic needle group and a servo motor, the servo motor is fixedly connected with the piston of the plastic needle group through a connecting screw, and the needle head of the plastic needle group is installed adjacent to the needle head of the pipetting needle group.

进一步地，热封装置为自动热封机，包括机架和固定在机架上的卷膜机构、热封机构、切割机构；Further, the heat-sealing device is an automatic heat-sealing machine, comprising a frame and a film rolling mechanism, a heat-sealing mechanism, and a cutting mechanism fixed on the frame;

热封机构包括运动连板、热封模块、热封弹簧、热封板和下托板，下托板水平固定在机架上，且下托板的一端与水平传送轴带连接，热封板设置在下托板的正上方，且热封板的上侧面通过热封弹簧与运动连板固定连接，运动连板与热封模块固定连接；The heat-sealing mechanism includes a moving connecting plate, a heat-sealing module, a heat-sealing spring, a heat-sealing plate and a lower support plate. It is arranged just above the lower support plate, and the upper side of the heat-sealing plate is fixedly connected to the moving connecting plate through the heat-sealing spring, and the moving connecting plate is fixedly connected to the heat-sealing module;

卷膜机构的热封膜设置在下托板与热封板之间；The heat-sealing film of the film rolling mechanism is arranged between the lower support plate and the heat-sealing plate;

切割机构包括横向切割组件和纵向切割组件，横向切割组件与运动连板固定连接，纵向切割组件与下托板固定连接，横向切割组件与水平传送轴带垂直设置，纵向切割组件与水平传送轴带平行设置。The cutting mechanism includes a transverse cutting assembly and a longitudinal cutting assembly. The transverse cutting assembly is fixedly connected with the moving connecting plate, and the longitudinal cutting assembly is fixedly connected with the lower support plate. Parallel setup.

进一步地，数据采集装置还包括扫码枪，试剂槽和孔板上均贴有图像信息，扫码枪用于录入、追踪及匹配试剂槽和孔板上的图像信息，扫码枪的数据信号端与数据分析模块的数据信号端连接。Further, the data acquisition device also includes a code scanning gun, the reagent tank and the well plate are affixed with image information, the code scanning gun is used to input, track and match the image information on the reagent tank and the orifice plate, and the data signal of the code scanning gun is used. The terminal is connected with the data signal terminal of the data analysis module.

进一步地，第一移动装置、第二移动装置和第三移动装置均包括驱动件、导轨和移动板，第一移动装置的导轨设置在水浴箱的上方，第三移动装置的导轨与多个光源发射装置发射光源的方向平行，第二移动装置的导轨与第三移动装置的导轨垂直，每个移动板可移动安装在每个导轨内，驱动件用于驱动移动板在导轨内的移动，热封后的反应板置于第一移动装置的移动板上，水浴后的反应板置于第二移动装置的移动板上，信息采集装置与第三移动装置的移动板固定连接。Further, the first moving device, the second moving device and the third moving device all include a driving member, a guide rail and a moving plate, the guide rail of the first moving device is arranged above the water bath box, and the guide rail of the third moving device is connected with a plurality of light sources. The direction of the light source emitted by the emitting device is parallel, the guide rail of the second moving device is perpendicular to the guide rail of the third moving device, each moving board can be movably installed in each guide rail, and the driving part is used to drive the moving board to move in the guide rail. The sealed reaction plate is placed on the mobile plate of the first mobile device, the reaction plate after water bath is placed on the mobile plate of the second mobile device, and the information acquisition device is fixedly connected with the mobile plate of the third mobile device.

进一步地，基因分型设备还包括三个操作显示屏，三个操作显示屏分别安装在移样装置、水浴装置和数据采集装置上，安装在移样装置上的操作显示屏的信号端与移样PLC模块的信号端连接，安装在水浴装置上的操作显示屏的信号端与水浴PLC模块的信号端连接，安装在数据采集装置上的操作显示屏的信号端与采集PLC模块的信号端连接。Further, the genotyping equipment also includes three operation display screens. The three operation display screens are respectively installed on the sample transfer device, the water bath device and the data acquisition device. The signal terminal of the sample PLC module is connected, the signal terminal of the operation display screen installed on the water bath device is connected to the signal terminal of the water bath PLC module, and the signal terminal of the operation display screen installed on the data acquisition device is connected to the signal terminal of the acquisition PLC module. .

进一步地，移样装置还包括阵列式移液吸头清洗装置，阵列式移液吸头清洗装置安装在非接触式移液装置和接触式移液装置的下方，用于清洗移液针组和塑料针组，包括清洗槽、水位监测装置和超声波振子，清洗槽上连接有进水管和出水管，水位监测装置固定设置在清洗槽内，超声波振子固定设置在清洗槽底部。Further, the sample pipetting device further includes an array type pipette tip cleaning device, and the array type pipette tip cleaning device is installed below the non-contact type pipetting device and the contact type pipetting device, and is used for cleaning the pipetting needle set and The plastic needle group includes a cleaning tank, a water level monitoring device and an ultrasonic vibrator. The cleaning tank is connected with a water inlet pipe and a water outlet pipe. The water level monitoring device is fixedly arranged in the cleaning tank, and the ultrasonic oscillator is fixedly arranged at the bottom of the cleaning tank.

一种自动化基因分型方法，包括以下步骤：An automated genotyping method comprising the following steps:

S1、调试好移样装置，操作安装在移样装置上的操作显示屏进行移液程序的设置；S1. Debug the sample transfer device, and operate the operation display screen installed on the sample transfer device to set the pipetting program;

S2、配置反应用的试剂，加入试剂槽后贴上图像信息；S2, configure the reagents for the reaction, and paste the image information after adding the reagent tank;

S3、将DNA样品分别加入到孔板，并贴上图像信息；S3. Add the DNA samples to the well plate respectively, and paste the image information;

S4、将反应板置于移液位置上；S4, place the reaction plate on the pipetting position;

S5、选择对应的移液方案，扫码枪扫描试剂槽和孔板的图像信息，操作安装在移样装置上的显示屏开始进行移液程序，移液装置先将孔板内的DNA样品加入到反应板内对应的1/2/3/4号孔位，移液装置再将试剂加入到反应板内，自动热封机封装完成移液混合的反应板；S5. Select the corresponding pipetting scheme, scan the image information of the reagent tank and the well plate with the barcode scanner, and operate the display screen installed on the sample transfer device to start the pipetting procedure. The pipetting device first adds the DNA sample in the well plate into the well plate. To thecorresponding hole 1/2/3/4 in the reaction plate, the pipetting device adds the reagents into the reaction plate, and the automatic heat sealer seals the reaction plate that has completed the pipetting and mixing;

S6、将封装好的反应板放置在第一移动装置的移动板上，第一移动装置将反应板移入水浴箱中，操作安装在水浴扩增装置上的操作显示屏创建、编辑或选择对应的PCR反应体系，执行选定的PCR扩增程序，液控装置对各水浴箱进行液位调控，温控装置共同调节各水浴箱内的温度环境，完成PCR反应后，第一移动装置将反应板移出水浴箱；S6. Place the packaged reaction plate on the mobile plate of the first mobile device, the first mobile device moves the reaction plate into the water bath box, and operates the operation display screen installed on the water bath amplification device to create, edit or select the corresponding The PCR reaction system executes the selected PCR amplification program, the liquid control device controls the liquid level of each water bath, and the temperature control device jointly adjusts the temperature environment in each water bath. After the PCR reaction is completed, the first mobile device puts the reaction plate Remove from the water bath;

S7、将完成PCR反应的反应板，放在数据采集装置中第二移动装置的移动板上，操作安装在数据采集装置上的操作显示屏，开始执行采集PLC模块的数据采集程序；S7, place the reaction plate that completes the PCR reaction on the mobile plate of the second mobile device in the data acquisition device, operate the operation display screen installed on the data acquisition device, and start to execute the data acquisition program of the acquisition PLC module;

S8、利用数据分析模块，导出扫码枪及数据采集装置的数据并匹配设定好的实验方案，读取样品分型结果。S8. Use the data analysis module to export the data of the code scanning gun and the data acquisition device, match the set experimental plan, and read the sample typing results.

进一步地，在S5中还包括：Further, S5 also includes:

S51、阵列式移液吸头清洗装置清洗移液针组的针头。S51, the array-type pipetting tip cleaning device cleans the needles of the pipetting needle group.

本发明的有益效果在于：通过移样装置、水浴装置和数据采集装置可以实现在样品量大、实验操作人员少、要求高的使用需求，通过非接触式移液装置和接触式移液装置的作用，可以实现对DNA样品和试剂的超微量移动，通过阵列式移液吸头清洗装置的作用，可以防止在移液过程中可能与试剂发生接触造成污染的情况，保证实验结果的有效性；通过水浴装置的作用，可实现温度快速变化及精准控制，从而能够缩短循环时间并提高扩增效率，通过扫码枪的作用，可以准确的录入、追踪和匹配相关的图像信息，防止出现匹配错误的情况；通过本设备的使用可以节约大量的人力，提高实验结果的准确性。The beneficial effects of the present invention are: the sample pipetting device, the water bath device and the data acquisition device can realize the use requirements of large sample volume, few experimental operators and high requirements, and the non-contact pipetting device and the contact type pipetting device It can realize the ultra-micro movement of DNA samples and reagents, and through the function of the array-type pipetting tip cleaning device, it can prevent the possibility of contact with the reagents during the pipetting process and cause contamination, and ensure the validity of the experimental results; Through the function of the water bath device, rapid temperature changes and precise control can be achieved, which can shorten the cycle time and improve the amplification efficiency. Through the function of the code scanning gun, the relevant image information can be accurately entered, tracked and matched to prevent matching errors. The use of this equipment can save a lot of manpower and improve the accuracy of the experimental results.

附图说明Description of drawings

图1是本发明一种自动化基因分型方法中实验流程示意图；Fig. 1 is a schematic diagram of experimental flow in a kind of automatic genotyping method of the present invention;

图2是本发明一种自动化基因分型设备中移样装置的结构示意图；Fig. 2 is the structural representation of the sample transfer device in a kind of automatic genotyping equipment of the present invention;

图3是本发明一种自动化基因分型设备中水浴装置的结构示意图；Fig. 3 is the structural representation of the water bath device in a kind of automatic genotyping equipment of the present invention;

图4是本发明一种自动化基因分型设备中数据采集装置的结构示意图；4 is a schematic structural diagram of a data acquisition device in an automated genotyping device of the present invention;

其中相应的附图标记为：The corresponding reference numbers are:

1-非接触式移液装置，2-自动热封机，3-阵列式移液吸头清洗装置，4-操作显示屏，5-水浴箱，6-水泵，7-液位检测器，8-温度传感器，9-加热水箱，10-导轨，11-移动板，12-信息采集装置，13-光源发射装置，14-接触式移液装置。1-Non-contact pipetting device, 2-Automatic heat sealer, 3-Array type pipette tip cleaning device, 4-Operation display screen, 5-Water bath tank, 6-Water pump, 7-Liquid level detector, 8 -Temperature sensor, 9-heated water tank, 10-guide rail, 11-moving plate, 12-information acquisition device, 13-light emission device, 14-contact pipetting device.

具体实施方式Detailed ways

下面结合附图对本发明作进一步说明：The present invention will be further described below in conjunction with the accompanying drawings:

如图2、图3、图4所示，一种自动化基因分型设备，包括用于移动试剂或DNA样品的移样装置、用于扩增PCR的水浴装置、采集各项数据的数据采集装置、移样PLC模块、水浴PLC模块、采集PLC模块和数据分析模块；As shown in Figure 2, Figure 3 and Figure 4, an automated genotyping equipment includes a sample transfer device for moving reagents or DNA samples, a water bath device for amplifying PCR, and a data acquisition device for collecting various data , Sampling PLC module, water bath PLC module, acquisition PLC module and data analysis module;

移样装置包括用于移动试剂的非接触式移液装置1、用于移动DNA样品的接触式移液装置14、装有试剂的试剂槽、装有DNA样品的孔板和热封装置，非接触式移液装置1包括反应板，非接触式移液装置1用于移动试剂槽内的试剂或孔板内的DNA样品，热封装置用于热封装有试剂和DNA样品的反应板，非接触式移液装置1与接触式移液装置14固定连接，移样PLC模块的信号端分别与热封装置、非接触式移液装置1和接触式移液装置14的信号端连接；The sample pipetting device includes anon-contact pipetting device 1 for moving reagents, acontact pipetting device 14 for moving DNA samples, a reagent tank containing reagents, a well plate containing DNA samples, and a heat-sealing device. Thecontact pipetting device 1 includes a reaction plate, thenon-contact pipetting device 1 is used for moving the reagent in the reagent tank or the DNA sample in the well plate, the heat sealing device is used for heat sealing the reaction plate with the reagent and the DNA sample, and thenon-contact pipetting device 1 is used for moving the reagent in the reagent tank or the DNA sample in the well plate. The contacttype pipetting device 1 is fixedly connected with the contacttype pipetting device 14, and the signal end of the sample pipetting PLC module is respectively connected with the signal ends of the heat sealing device, the non-contacttype pipetting device 1 and the contacttype pipetting device 14;

水浴装置包括水浴箱5、用于移动热封后反应板的第一移动装置、控制水浴箱5水位的液控装置和用于控制水浴箱5内温度的温控装置，热封后的反应板置于第一移动装置的移动端，第一移动装置设置在水浴箱5的上方，将反应板在水浴箱5内移进移出，水浴PLC模块的信号端分别与温控装置、液控装置和第一移动装置的信号端连接；The water bath device includes a water bath box 5, a first moving device for moving the reaction plate after heat sealing, a hydraulic control device for controlling the water level of the water bath box 5, and a temperature control device for controlling the temperature in the water bath box 5. The reaction plate after heat sealing Placed on the mobile end of the first mobile device, the first mobile device is arranged above the water bath box 5, the reaction plate is moved in and out of the water bath box 5, the signal end of the water bath PLC module is respectively connected with the temperature control device, the liquid control device and the The signal terminal of the first mobile device is connected;

液控装置包括水泵6和液位检测器7，温控装置包括温度传感器8和加热水箱9，水泵6的出水口与水浴箱5的进水口连通，液位监测器7用于检测水浴箱5内的液位，加热水箱9用于升温水浴箱5内的水温，温度传感器8用于检测水浴箱5内的水温，液位检测器7和温度传感器8的数据信号输出端均与水浴PLC模块的数据信号输入端连接，水浴PLC模块的控制信号输出端分别与水泵6和加热水箱9的控制信号输出端连接。The liquid control device includes awater pump 6 and aliquid level detector 7, and the temperature control device includes atemperature sensor 8 and aheating water tank 9. The water outlet of thewater pump 6 is communicated with the water inlet of the water bath tank 5, and the liquid level monitor 7 is used to detect the water bath tank 5. The liquid level inside, theheating water tank 9 is used to heat up the water temperature in the water bath 5, thetemperature sensor 8 is used to detect the water temperature in the water bath 5, and the data signal output ends of theliquid level detector 7 and thetemperature sensor 8 are connected with the water bath PLC module. The data signal input end of the water bath PLC module is connected to the control signal output end of the water bath PLC module and the control signal output end of thewater pump 6 and theheating water tank 9 are respectively connected.

数据采集装置用于移动水浴后反应板的第二移动装置、多个光源发射装置13、用于采集反应板在不同光源下的信息采集装置12、用于移动的信息采集装置12的第三移动装置，水浴后的反应板置于第二移动装置的移动端，第二移动装置将反应板移动到不同的光信息采集位，信息采集装置12与第三移动装置的移动端固定连接，第三移动装置将信息采集装置12移动到不同的光信息采集位，信息采集装置12对反应板在不同光源下的光信息进行采集，多个光源发射装置13位于同一平面上，第三移动装置设置在多个光源发射装置13的下方，第二移动装置设置在第三移动装置的下方，采集PLC模块的信号端分别与第二移动装置、第三移动装置、信息采集装置12和光源发射装置13的信号端连接；The data acquisition device is used to move the second moving device of the reaction plate after the water bath, a plurality of lightsource emission devices 13, theinformation acquisition device 12 for collecting the reaction plate under different light sources, and the third movement of theinformation acquisition device 12 for moving device, the reaction plate after the water bath is placed on the mobile end of the second mobile device, the second mobile device moves the reaction plate to different optical information acquisition positions, theinformation acquisition device 12 is fixedly connected with the mobile end of the third mobile device, and the third mobile device The mobile device moves theinformation acquisition device 12 to different optical information acquisition positions, theinformation acquisition device 12 collects the optical information of the reaction plate under different light sources, the plurality of lightsource emission devices 13 are located on the same plane, and the third mobile device is arranged on Below the plurality of lightsource emission devices 13, the second mobile device is arranged below the third mobile device, and the signal terminals of the acquisition PLC module are respectively connected with the second mobile device, the third mobile device, theinformation acquisition device 12 and the lightsource emission device 13. signal terminal connection;

数据分析模块的数据信号端与信息采集装置12的数据信号端连接，数据分析模块安装在计算机内，数据分析模块为基因分型数据综合分析管理软件。The data signal end of the data analysis module is connected with the data signal end of theinformation acquisition device 12 , the data analysis module is installed in the computer, and the data analysis module is a comprehensive analysis and management software for genotyping data.

信息采集装置12为相机。Theinformation collection device 12 is a camera.

接触式移液装置14包括塑料针组和伺服电机，伺服电机通过连接螺丝与塑料针组的活塞固定连接，塑料针组的针头与移液针组的针头相邻安装。Thecontact pipetting device 14 includes a plastic needle group and a servo motor. The servo motor is fixedly connected to the piston of the plastic needle group through a connecting screw, and the needle head of the plastic needle group is installed adjacent to the needle head of the pipetting needle group.

数据采集装置还包括扫码枪，试剂槽和孔板上均贴有图像信息，扫码枪用于录入、追踪及匹配试剂槽和孔板上的图像信息，扫码枪的数据信号端与数据分析模块的数据信号端连接。The data acquisition device also includes a scan code gun. Image information is pasted on the reagent tank and the well plate. The scan code gun is used to input, track and match the image information on the reagent tank and the well plate. The data signal terminal of the scan code gun and the data The data signal terminal of the analysis module is connected.

如图2、图3、图4所示，第一移动装置、第二移动装置和第三移动装置均包括驱动件、导轨10和移动板11，第一移动装置的导轨10设置在水浴箱5的上方，第三移动装置的导轨10与多个光源发射装置13发射光源的方向平行，第二移动装置的导轨10与第三移动装置的导轨10垂直，每个移动板可移动安装在每个导轨内，移动板11可移动安装在导轨10内，驱动件用于驱动移动板11在导轨10内的移动，热封后的反应板置于第一移动装置的移动板11上，水浴后的反应板置于第二移动装置的移动板11上，信息采集装置12与第三移动装置的移动板11固定连接。As shown in FIG. 2 , FIG. 3 and FIG. 4 , the first moving device, the second moving device and the third moving device each include a driving member, aguide rail 10 and a moving plate 11 , and theguide rail 10 of the first moving device is arranged on the water bath box 5 Above, theguide rail 10 of the third mobile device is parallel to the direction in which the light sources are emitted by the plurality of lightsource emitting devices 13, theguide rail 10 of the second mobile device is perpendicular to theguide rail 10 of the third mobile device, and each moving board can be movably installed on each In the guide rail, the moving plate 11 can be movably installed in theguide rail 10, and the driving member is used to drive the moving plate 11 to move in theguide rail 10. The heat-sealed reaction plate is placed on the moving plate 11 of the first moving device. The reaction plate is placed on the moving plate 11 of the second moving device, and theinformation collecting device 12 is fixedly connected to the moving plate 11 of the third moving device.

如图2、图3、图4所示，基因分型设备还包括三个操作显示屏4，三个操作显示屏4分别安装在移样装置、水浴装置和数据采集装置上，安装在移样装置上的操作显示屏4的信号端与移样PLC模块的信号端连接，安装在水浴装置上的操作显示屏4的信号端与水浴PLC模块的信号端连接，安装在数据采集装置上的操作显示屏4的信号端与采集PLC模块的信号端连接。As shown in Fig. 2, Fig. 3, Fig. 4, the genotyping equipment also includes threeoperation display screens 4, and the threeoperation display screens 4 are respectively installed on the sample transfer device, the water bath device and the data acquisition device, and are installed on the sample transfer device. The signal terminal of theoperation display screen 4 on the device is connected with the signal terminal of the sample transfer PLC module, and the signal terminal of theoperation display screen 4 installed on the water bath device is connected with the signal terminal of the water bath PLC module. The signal end of thedisplay screen 4 is connected with the signal end of the acquisition PLC module.

移样装置还包括阵列式移液吸头清洗装置3，阵列式移液吸头清洗装置3是引用申请号为CN201810507814.2的专利，阵列式移液吸头清洗装置3安装在非接触式移液装置1和接触式移液装置14的下方，用于清洗移液针组和塑料针组，包括清洗槽、水位监测装置和超声波振子，清洗槽上连接有进水管和出水管，水位监测装置固定设置在清洗槽内，超声波振子固定设置在清洗槽底部。The sample pipetting device also includes an array-type pipettetip cleaning device 3, which is a patent cited with application number CN201810507814.2, and the array-type pipettetip cleaning device 3 is installed in a non-contact pipetting The bottom of theliquid device 1 and thecontact pipetting device 14 is used to clean the pipetting needle group and the plastic needle group, including the cleaning tank, the water level monitoring device and the ultrasonic vibrator. The cleaning tank is connected with a water inlet pipe and a water outlet pipe, and a water level monitoring device. It is fixedly arranged in the cleaning tank, and the ultrasonic vibrator is fixedly arranged at the bottom of the cleaning tank.

如图2所示，阵列式移液吸头清洗装置3还包括进水槽和出水槽；As shown in FIG. 2 , the array-type pipettetip cleaning device 3 further includes a water inlet tank and a water outlet tank;

进水槽固定设置在清洗槽的第一侧面，进水槽与清洗槽之间通过设置在进水槽和清洗槽上部的小孔连通，进水管与进水槽连通；The water inlet tank is fixedly arranged on the first side of the cleaning tank, the water inlet tank is communicated with the cleaning tank through the small holes arranged on the upper part of the water inlet tank and the cleaning tank, and the water inlet pipe is communicated with the water inlet tank;

出水槽设置在清洗槽的第二侧面，第二侧面与第一侧面相对设置，出水槽通过出水口与清洗槽连通，排水管与出水槽连通。The water outlet tank is arranged on the second side of the cleaning tank, the second side is arranged opposite to the first side, the water outlet tank is communicated with the cleaning tank through the water outlet, and the drain pipe is communicated with the water outlet tank.

清洗槽的底面倾斜设置，且沿进水槽朝向出水槽的方向下倾斜，清洗槽的底面的倾斜角度为1°～5°。The bottom surface of the cleaning tank is inclined and inclined downward along the direction of the water inlet tank toward the water outlet tank, and the inclination angle of the bottom surface of the cleaning tank is 1° to 5°.

清洗槽的下方设置有电路槽，超声波振子的驱动电路固定设置在电路槽内，超声波振子穿过清洗槽的底面设置在清洗槽的底面中心处，且超声波振子与清洗槽的底面密封连接。A circuit slot is arranged below the cleaning tank, the driving circuit of the ultrasonic vibrator is fixedly arranged in the circuit groove, the ultrasonic vibrator is arranged at the center of the bottom surface of the cleaning tank through the bottom surface of the cleaning tank, and the ultrasonic vibrator is sealed with the bottom surface of the cleaning tank.

清洗槽的顶部设置有顶盖，顶盖上设置有供移液洗头穿过的条形通槽。The top of the cleaning tank is provided with a top cover, and the top cover is provided with a bar-shaped through groove for the pipetting shampoo to pass through.

水位监测装置包括高水位监测接线柱和低水位监测接线柱，高水位监测接线柱安装在清洗槽的上部，低水位监测接线柱安装在出水槽的下部。The water level monitoring device includes a high water level monitoring terminal and a low water level monitoring terminal.

进水管和出水管上均设置有电磁阀，电磁阀的信号端与水位监测装置的信号端电连接。The water inlet pipe and the water outlet pipe are both provided with solenoid valves, and the signal end of the solenoid valve is electrically connected with the signal end of the water level monitoring device.

非接触式移液装置1是引用申请号CN201810614967.7的专利，非接触式移液装置1还包括移液模块、平移组件和控制模块，平移组件固定设置在移液位置的上方，反应板置于移液位置上，移液模块的移液端与平移组件的移动端固定连接，控制组件与移液模块连接；移液模块包括移液针组、溶液罐、吸液泵、气压泵和压力控制容器，溶液罐分别与移液针组、吸液泵和压力控制容器连通，压力控制容器与气压泵连通；控制模块包括高频电磁阀、第一电磁阀、第二电磁阀、第三电磁阀和比例阀，高频电磁阀设置在移液针组与溶液罐之间的液路上，第一电磁阀设置在溶液罐与压力控制容器之间的液路上，第二电磁阀设置在吸液泵与溶液罐之间的液路上，第三电磁阀设置在吸液泵与压力控制容器之间的液路上，比例阀设置在压力控制容器与气压泵之间的气路上反应板上设置有多个反应孔组，反应孔组包括多个呈直线分布的反应孔，且相邻的两个反应孔组之间的距离相等，反应孔组与平移组件移动方向垂直。Thenon-contact pipetting device 1 is a patent cited with the application number CN201810614967.7. Thenon-contact pipetting device 1 also includes a pipetting module, a translation component and a control module. The translation component is fixedly arranged above the pipetting position, and the reaction plate is set. In the pipetting position, the pipetting end of the pipetting module is fixedly connected with the moving end of the translation component, and the control component is connected with the pipetting module; the pipetting module includes a pipetting needle group, a solution tank, a suction pump, an air pressure pump and a pressure pump. The control container, the solution tank is respectively communicated with the pipetting needle group, the suction pump and the pressure control container, and the pressure control container is communicated with the air pressure pump; the control module includes a high-frequency solenoid valve, a first solenoid valve, a second solenoid valve, and a third solenoid valve. Valve and proportional valve, the high-frequency solenoid valve is set on the liquid path between the pipetting needle group and the solution tank, the first solenoid valve is set on the liquid path between the solution tank and the pressure control container, and the second solenoid valve is set on the liquid suction On the liquid path between the pump and the solution tank, the third solenoid valve is set on the liquid path between the suction pump and the pressure control container, and the proportional valve is set on the gas path between the pressure control container and the air pressure pump. A reaction well group, the reaction well group includes a plurality of reaction wells distributed in a straight line, and the distance between two adjacent reaction well groups is equal, and the reaction well group is perpendicular to the moving direction of the translation component.

反应板上设置有多个反应孔组，反应孔组包括多个呈直线分布的反应孔，且相邻的两个反应孔组之间的距离相等，反应孔组与平移组件移动方向垂直。A plurality of reaction well groups are arranged on the reaction plate, the reaction well groups include a plurality of reaction wells distributed in a straight line, and the distances between two adjacent reaction well groups are equal, and the reaction well groups are perpendicular to the moving direction of the translation component.

平移组件为液压杆、气压杆或直线电机。The translation components are hydraulic rods, pneumatic rods or linear motors.

压力控制容器下部为溶液、上部为空气，气压泵与压力控制容器的上部连通，压力控制容器的上部连接有排气头，排气头与压力控制容器之间的气路上设置有第四电磁阀。The lower part of the pressure control container is the solution, the upper part is the air, the air pressure pump is communicated with the upper part of the pressure control container, the upper part of the pressure control container is connected with an exhaust head, and the air path between the exhaust head and the pressure control container is provided with a fourth solenoid valve .

自动热封机2是引用申请号为CN201810509278.X的专利，包括机架和固定在机架上的卷膜机构、热封机构、切割机构；The automaticheat sealing machine 2 is a patent cited with the application number CN201810509278.X, including a frame and a film rolling mechanism, a heat sealing mechanism, and a cutting mechanism fixed on the frame;

热封机构包括运动连板、热封模块、热封弹簧、热封板和下托板，下托板水平固定在机架上，且下托板的一端与水平传送轴带连接，热封板设置在下托板的正上方，且热封板的上侧面通过热封弹簧与运动连板固定连接，运动连板与热封模块固定连接；The heat-sealing mechanism includes a moving connecting plate, a heat-sealing module, a heat-sealing spring, a heat-sealing plate and a lower support plate. It is arranged just above the lower support plate, and the upper side of the heat-sealing plate is fixedly connected to the moving connecting plate through the heat-sealing spring, and the moving connecting plate is fixedly connected to the heat-sealing module;

卷膜机构的热封膜设置在下托板与热封板之间；The heat-sealing film of the film rolling mechanism is arranged between the lower support plate and the heat-sealing plate;

切割机构包括横向切割组件和纵向切割组件，横向切割组件与运动连板固定连接，纵向切割组件与下托板固定连接，横向切割组件与水平传送轴带垂直设置，纵向切割组件与水平传送轴带平行设置。The cutting mechanism includes a transverse cutting assembly and a longitudinal cutting assembly. The transverse cutting assembly is fixedly connected with the moving connecting plate, and the longitudinal cutting assembly is fixedly connected with the lower support plate. Parallel setup.

卷膜机构包括薄膜卷盘、收膜卷盘、卷膜电机和导杆，两个导杆平行设置在下托板的两侧，且导杆与机架可转动连接，热封膜的第一端与薄膜卷盘固定连接，且热封板卷绕在薄膜卷盘上，热封膜的第二端依次绕过两个导杆缠绕在收膜卷盘上，卷膜电机通过变速箱分别与薄膜卷盘和收膜卷边的转矩输入端固定连接。The film winding mechanism includes a film reel, a film winding reel, a film winding motor and a guide rod. The two guide rods are arranged in parallel on both sides of the lower pallet, and the guide rods are rotatably connected to the frame. The first end of the heat-sealing film is It is fixedly connected with the film reel, and the heat-sealing plate is wound on the film reel, the second end of the heat-sealing film is wound around the two guide rods in turn on the film reel, and the film winding motor is connected to the film through the gearbox. The torque input end of the reel and the winder are fixedly connected.

热封模块包括升降装置、导柱和导套，导套的下端与运动连板的上侧面固定连接，导柱的下端设置在导套内且可沿导套上下滑动，导套的上端与机架固定连接，升降装置的固定端与机架固定连接，升降装置的升降端与运动连板固定连接。The heat sealing module includes a lifting device, a guide post and a guide sleeve. The lower end of the guide sleeve is fixedly connected to the upper side of the moving connecting plate. The lower end of the guide post is set in the guide sleeve and can slide up and down along the guide sleeve. The frame is fixedly connected, the fixed end of the lifting device is fixedly connected with the frame, and the lifting end of the lifting device is fixedly connected with the moving connecting plate.

下托板的上侧面设置有尺寸等于样品板的尺寸的凹槽，样品板通过水平传送轴带运输至样品板的凹槽内。The upper side of the lower pallet is provided with a groove with a size equal to the size of the sample plate, and the sample plate is transported into the groove of the sample plate by a horizontal conveyor belt.

横向切割组件包括横向移动电机、横向移动导轨和横向切刀，横向移动导轨与运动连板固定连接，横向切刀与横向移动电机固定连接，横向移动电机与横向移动导轨可移动连接；The transverse cutting assembly includes a transverse movement motor, a transverse movement guide and a transverse cutter, the transverse movement guide is fixedly connected with the moving connecting plate, the transverse cutter is fixedly connected with the transverse movement motor, and the transverse movement motor is movably connected with the transverse movement guide;

纵向切割组件包括纵向移动电机、纵向移动导轨和纵向切刀，纵向移动导轨与下托板固定连接，纵向切刀与纵向移动电机固定连接，纵向移动电机与纵向移动导轨可移动连接。The longitudinal cutting assembly includes a longitudinal moving motor, a longitudinal moving guide rail and a longitudinal cutting knife, the longitudinal moving guide rail is fixedly connected with the lower support plate, the longitudinal cutting knife is fixedly connected with the longitudinal moving motor, and the longitudinal moving motor is movably connected with the longitudinal moving guide rail.

纵向切刀的数量为两个，两个纵向切刀分别设置在下托板的凹槽的两侧，且切割方向与水平传送轴带的移动方向相同；The number of longitudinal cutting knives is two, and the two longitudinal cutting knives are respectively arranged on both sides of the groove of the lower pallet, and the cutting direction is the same as the moving direction of the horizontal conveying shaft belt;

横向切刀的数量为两个，两个横向切刀分别设置在运动连板的两端，且两个横向切刀分别位于样品板的凹槽的两端的正上方，横向切刀的切割方向与水平传送带的移动方向垂直。The number of transverse cutting knives is two, the two transverse cutting knives are respectively arranged at both ends of the moving connecting plate, and the two transverse cutting knives are respectively located directly above the two ends of the groove of the sample plate, and the cutting direction of the transverse cutting knives is the same as The moving direction of the horizontal conveyor belt is vertical.

切割机构还包括感应器，感应器固定设置在机架上，且感应器的感应方向竖直向下朝向位于热封板和下托板之间的热封膜，热封膜上设置有多个感应点，感应器的信号端与卷膜机构的控制端电连接。The cutting mechanism also includes an inductor, the inductor is fixedly arranged on the frame, and the sensing direction of the inductor is vertically downward toward the heat-sealing film located between the heat-sealing plate and the lower support plate, and a plurality of heat-sealing films are arranged on the heat-sealing film. Induction point, the signal end of the sensor is electrically connected with the control end of the film winding mechanism.

如图1所示，一种自动化基因分型方法，包括以下步骤：As shown in Figure 1, an automated genotyping method includes the following steps:

S1、调试好移样装置，操作安装在移样装置上的操作显示屏进行移液程序的设置；S1. Debug the sample transfer device, and operate the operation display screen installed on the sample transfer device to set the pipetting program;

S2、配置反应用的试剂，加入试剂槽，每个槽位100-8000微升，贴上图像信息，将试剂槽放置于对应的试剂槽放置位；S2. Configure the reagents for the reaction, add 100-8000 microliters of reagents to each slot, paste the image information, and place the reagent slot in the corresponding reagent slot;

S3、将DNA样品分别加入到孔板，每个孔位10-100微升，并贴上图像信息，将孔板放置于对应的孔板放置位；S3. Add the DNA samples to the well plate respectively, 10-100 microliters per well, paste the image information, and place the well plate in the corresponding well plate placement position;

S4、将反应板置于移液位置上；S4, place the reaction plate on the pipetting position;

S5、选择对应的移液方案，扫码枪扫描试剂槽和孔板的图像信息，操作安装在移样装置上的显示屏开始进行移液程序，移液装置先将孔板内的DNA样品加入到反应板内对应的1/2/3/4号孔位，每个孔位0.5-2微升，移液装置再将试剂加入到反应板内，每个孔位0.2-1.5微升，自动热封机2封装完成移液混合的反应板，阵列式移液吸头清洗装置3清洗移液针组的针头；S5. Select the corresponding pipetting scheme, scan the image information of the reagent tank and the well plate with the barcode scanner, and operate the display screen installed on the sample transfer device to start the pipetting procedure. The pipetting device first adds the DNA sample in the well plate into the well plate. To the correspondingwells 1/2/3/4 in the reaction plate, each well is 0.5-2 μl, and the pipetting device adds the reagents into the reaction plate, 0.2-1.5 μl per well, automatically Theheat sealer 2 encapsulates the reaction plate that has completed the pipetting and mixing, and the array-type pipettingtip cleaning device 3 cleans the needles of the pipetting needle group;

S6、将封装好的反应板放置在第一移动装置的移动板11上，每次最多可放置20块反应板，第一移动装置将反应板移入水浴箱5中，操作安装在水浴扩增装置上的操作显示屏4创建、编辑或选择对应的PCR反应体系，执行行选定的PCR扩增程序，液控装置对各水浴箱5进行液位调控，温控装置共同调节各水浴箱5内的温度环境，完成PCR反应后，第一移动装置将反应板移出水浴箱5；S6. Place the encapsulated reaction plates on the moving plate 11 of the first mobile device, and a maximum of 20 reaction plates can be placed at a time. The first mobile device moves the reaction plates into the water bath box 5, and installs them in the water bath amplification device. Create, edit or select the corresponding PCR reaction system on theoperation display screen 4 on the upper panel, execute the selected PCR amplification program, the liquid control device controls the liquid level of each water bath 5, and the temperature control device jointly adjusts the inside of each water bath 5. temperature environment, after completing the PCR reaction, the first moving device removes the reaction plate from the water bath 5;

S7、将完成PCR反应的反应板，放在数据采集装置中第二移动装置的移动板11上，操作安装在数据采集装置上的操作显示屏4，开始执行采集PLC模块的数据采集程序，相机开始采集反应板在不同光源下的光信息；S7, place the reaction plate that has completed the PCR reaction on the mobile plate 11 of the second mobile device in the data acquisition device, operate theoperation display screen 4 installed on the data acquisition device, start to execute the data acquisition program of the acquisition PLC module, the camera Start to collect the light information of the reaction plate under different light sources;

S8、利用数据分析模块，导出扫码枪及数据采集装置的数据并匹配设定好的实验方案，读取样品分型结果。S8. Use the data analysis module to export the data of the code scanning gun and the data acquisition device, match the set experimental plan, and read the sample typing results.

通过移样装置、水浴装置和数据采集装置可以实现在样品量大、实验操作人员少、要求高的使用需求，通过非接触式移液装置1和接触式移液装置14的作用，可以实现对DNA样品和试剂的超微量移动，通过阵列式移液吸头清洗装置的作用，可以防止在移液过程中可能与试剂发生接触造成污染的情况，保证实验结果的有效性；通过水浴装置的作用，可实现温度快速变化及精准控制，从而能够缩短循环时间并提高扩增效率，通过扫码枪的作用，可以准确的录入、追踪和匹配相关的图像信息，防止出现匹配错误的情况；通过本设备的使用可以节约大量的人力，提高实验结果的准确性。Through the sample pipetting device, the water bath device and the data acquisition device, the use requirements of large sample volume, few experimental operators and high requirements can be realized. The ultra-trace movement of DNA samples and reagents can prevent contamination caused by contact with reagents during the pipetting process through the action of the array-type pipette tip cleaning device, and ensure the validity of the experimental results; through the action of the water bath device , which can achieve rapid temperature change and precise control, thereby shortening the cycle time and improving the amplification efficiency. Through the function of the code scanning gun, the relevant image information can be accurately entered, tracked and matched to prevent the occurrence of matching errors; The use of the equipment can save a lot of manpower and improve the accuracy of the experimental results.

本发明的技术方案不限于上述具体实施例的限制，凡是根据本发明的技术方案做出的技术变形，均落入本发明的保护范围之内。The technical solutions of the present invention are not limited to the limitations of the above-mentioned specific embodiments, and all technical modifications made according to the technical solutions of the present invention fall within the protection scope of the present invention.

Claims (9)

1. An automatic genotyping device is characterized by comprising a sample transferring device for transferring a reagent or a DNA sample, a water bath device for amplifying PCR, a data acquisition device for acquiring various data, a sample transferring PLC module, a water bath PLC module, an acquisition PLC module and a data analysis module;

the sample moving device comprises a non-contact type liquid moving device for moving a reagent, a contact type liquid moving device for moving a DNA sample, a reagent tank containing the reagent, a pore plate containing the DNA sample and a heat sealing device, the non-contact type liquid moving device comprises a reaction plate, the non-contact type liquid moving device is used for moving the reagent in the reagent tank or the DNA sample in the pore plate, the heat sealing device is used for heat sealing the reaction plate containing the reagent and the DNA sample, the non-contact type liquid moving device is fixedly connected with the contact type liquid moving device, and a signal end of the sample moving PLC module is respectively connected with signal ends of the heat sealing device, the non-contact type liquid moving device and the contact type liquid moving device;

the water bath device comprises a water bath box, a first moving device for moving the reaction plate after heat sealing, a hydraulic control device for controlling the water level of the water bath box and a temperature control device for controlling the temperature in the water bath box, wherein the reaction plate after heat sealing is arranged at the moving end of the first moving device, the first moving device is arranged above the water bath box and moves the reaction plate in and out of the water bath box, and the signal end of the water bath PLC module is respectively connected with the temperature control device, the hydraulic control device and the signal end of the first moving device;

The data acquisition device is used for moving a second moving device of the reaction plate after water bath, the plurality of light source emitting devices, an information acquisition device for acquiring information of the reaction plate under different light sources and a third moving device of the information acquisition device for movement, the reaction plate after water bath is arranged at the moving end of the second moving device, the second moving device moves the reaction plate to different light information acquisition positions, the information acquisition device is fixedly connected with the moving end of the third moving device, the third moving device moves the information acquisition device to different light information acquisition positions, the information acquisition device acquires light information of the reaction plate under different light sources, the plurality of light source emitting devices are positioned on the same plane, the third moving device is arranged below the plurality of light source emitting devices, the second moving device is arranged below the third moving device, and the signal acquisition end of the PLC module is respectively connected with the second moving device, The third mobile device, the information acquisition device and the signal end of the light source emission device are connected;

the data signal end of the data analysis module is connected with the data signal end of the information acquisition device;

the first moving device, the second moving device and the third moving device respectively comprise driving pieces, guide rails and moving plates, the guide rails of the first moving device are arranged above the water bath box, the guide rails of the third moving device are parallel to the direction of the light sources emitted by the light source emitting devices, the guide rails of the second moving device are perpendicular to the guide rails of the third moving device, each moving plate is movably arranged in each guide rail, the driving pieces are used for driving the moving plates to move in the guide rails, the reaction plates after heat sealing are arranged on the moving plates of the first moving device, the reaction plates after water bath are arranged on the moving plates of the second moving device, and the information acquisition devices are fixedly connected with the moving plates of the third moving device.

2. An automated genotyping apparatus according to claim 1, wherein the non-contact pipetting device further comprises a pipetting module, a translation assembly and a control module, the translation assembly is fixedly arranged above the pipetting position, the reaction plate is arranged on the pipetting position, the pipetting end of the pipetting module is fixedly connected with the moving end of the translation assembly, and the control assembly is connected with the pipetting module; the liquid transferring module comprises a liquid transferring needle group, a solution tank, a liquid sucking pump, a pneumatic pump and a pressure control container, wherein the solution tank is respectively communicated with the liquid transferring needle group, the liquid sucking pump and the pressure control container, and the pressure control container is communicated with the pneumatic pump; the control module comprises a high-frequency electromagnetic valve, a first electromagnetic valve, a second electromagnetic valve, a third electromagnetic valve and a proportional valve, the high-frequency electromagnetic valve is arranged on a liquid path between the liquid-transferring needle group and the solution tank, the first electromagnetic valve is arranged on a liquid path between the solution tank and the pressure control container, the second electromagnetic valve is arranged on a liquid path between the liquid-sucking pump and the solution tank, the third electromagnetic valve is arranged on a liquid path between the liquid-sucking pump and the pressure control container, and the proportional valve is arranged on a gas path between the pressure control container and the pneumatic pump.

3. An automated genotyping device according to claim 2, wherein the contact pipetting device comprises a plastic needle set and a servo motor, the servo motor is fixedly connected with the piston of the plastic needle set through a connecting screw, and the needle of the plastic needle set is arranged adjacent to the needle of the pipetting needle set.

4. An automatic genotyping device according to claim 1, wherein the heat sealing device is an automatic heat sealing machine comprising a frame, and a film winding mechanism, a heat sealing mechanism and a cutting mechanism which are fixed on the frame;

the heat sealing mechanism comprises a moving connecting plate, a heat sealing module, a heat sealing spring, a heat sealing plate and a lower supporting plate, the lower supporting plate is horizontally fixed on the rack, one end of the lower supporting plate is connected with the horizontal conveying shaft belt, the heat sealing plate is arranged right above the lower supporting plate, the upper side surface of the heat sealing plate is fixedly connected with the moving connecting plate through the heat sealing spring, and the moving connecting plate is fixedly connected with the heat sealing module;

the heat-sealing film of the film rolling mechanism is arranged between the lower supporting plate and the heat-sealing plate;

the cutting mechanism comprises a transverse cutting assembly and a longitudinal cutting assembly, the transverse cutting assembly is fixedly connected with the moving connecting plate, the longitudinal cutting assembly is fixedly connected with the lower supporting plate, the transverse cutting assembly is perpendicular to the horizontal conveying shaft belt, and the longitudinal cutting assembly is parallel to the horizontal conveying shaft belt.

5. The automatic genotyping device of claim 1, wherein the data acquisition device further comprises a code scanning gun, the reagent tank and the well plate are both attached with image information, the code scanning gun is used for inputting, tracking and matching the image information on the reagent tank and the well plate, and a data signal end of the code scanning gun is connected with a data signal end of the data analysis module.

6. The automatic genotyping equipment according to claim 1, wherein the genotyping equipment further comprises three operation display screens, the three operation display screens are respectively installed on the sample moving device, the water bath device and the data acquisition device, a signal end of the operation display screen installed on the sample moving device is connected with a signal end of the sample moving PLC module, a signal end of the operation display screen installed on the water bath device is connected with a signal end of the water bath PLC module, and a signal end of the operation display screen installed on the data acquisition device is connected with a signal end of the acquisition PLC module.

7. An automatic genotyping device according to claim 3, wherein the sample moving device further comprises an array type liquid-transferring tip cleaning device, the array type liquid-transferring tip cleaning device is installed below the non-contact type liquid-transferring device and the contact type liquid-transferring device and is used for cleaning the liquid-transferring needle group and the plastic needle group and comprises a cleaning tank, a water level monitoring device and an ultrasonic vibrator, a water inlet pipe and a water outlet pipe are connected to the cleaning tank, the water level monitoring device is fixedly arranged in the cleaning tank, and the ultrasonic vibrator is fixedly arranged at the bottom of the cleaning tank.

8. An automated genotyping method using an automated genotyping apparatus according to any one of claims 1 to 7, wherein the automated genotyping apparatus comprises: the method comprises the following steps:

S1, debugging the sample moving device, and operating an operation display screen arranged on the sample moving device to set a liquid moving program;

s2, preparing a reagent for reaction, adding the reagent into the reagent tank, and attaching image information;

s3, adding the DNA samples into the pore plates respectively, and attaching image information;

s4, placing the reaction plate on a liquid transferring position;

s5, selecting a corresponding pipetting scheme, scanning image information of the reagent tank and the orifice plate by the code scanning gun, operating a display screen arranged on the sample transferring device to start pipetting, adding the DNA sample in the orifice plate into a corresponding hole position No. 1/2/3/4 in the reaction plate by the pipetting device, adding the reagent into the reaction plate by the pipetting device, and packaging the reaction plate subjected to pipetting mixing by an automatic heat sealing machine;

s6, placing the packaged reaction plate on a moving plate of a first moving device, moving the reaction plate into a water bath box by the first moving device, operating an operation display screen arranged on a water bath amplification device to create, edit or select a corresponding PCR reaction system, executing a selected PCR amplification program, carrying out liquid level regulation and control on each water bath box by a liquid control device, jointly regulating the temperature environment in each water bath box by a temperature control device, and moving the reaction plate out of the water bath box by the first moving device after PCR reaction is completed;

S7, placing the reaction plate which completes the PCR reaction on a moving plate of a second moving device in the data acquisition device, operating an operation display screen arranged on the data acquisition device, starting to execute a data acquisition program of the PLC module, and starting to acquire the light information of the reaction plate under different light sources by the information acquisition device;

and S8, exporting the data of the code scanning gun and the data acquisition device by using the data analysis module, matching the set experimental scheme, and reading the sample typing result.

9. An automated genotyping method according to claim 8, further comprising, in S5:

s51, the array type pipetting tip cleaning device cleans the needle heads of the pipetting needle group.

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