The preparation method and microfluid drive of micro-fluidic chipTechnical field
The present invention relates to biochemical analysis technical fields, more particularly to the preparation method and microfluid of a kind of micro-fluidic chipDriving device.
Background technique
Micro-fluidic chip is a kind of novel analysis platform, can grasp the experiments such as sample preparation, reaction, separation, detectionBe integrated on the chip of one piece of square centimeter grade, have sample and reagent consumption less, analysis speed is fast and operation automation etc.Advantage.As the power source of fluid transmission, micro fluid dynamcis has become a key of micro-fluidic chip, receives extensive passNote.
Currently, Micropump of the micro fluid dynamcis frequently with outside, due to being subject to cross contamination, still in practical applicationIt faces big challenge.In recent years, it is driven studies have reported that microfluid may be implemented using microeffects such as capillarity, electrowettingsDynamic, flow velocity is can be controlled within the scope of μ L/min~mL/min, may alternatively be integrated on micro-fluidic chip.However, electrowetting Micropump orThe processing technology of capillary Micropump is very complicated, and at high cost.Therefore, simple, the inexpensive micro fluid dynamcis technology pair of researchMicro-fluidic chip research is of great significance.
Summary of the invention
(1) technical problems to be solved
The object of the present invention is to provide a kind of micro-flow control chip preparation method and microfluid drives, to solve on chipThe problem of micro fluid dynamcis design complexity, higher cost.
(2) technical solution
In order to solve the above technical problem, the present invention provides a kind of preparation methods of micro-fluidic chip, include the following steps:
Step 1, when setting is mixed by weight 20:1~15:1 in polydimethylsiloxane prepolymer object and curing agentBetween, it is put into vacuum drying ware and vacuumizes to remove the bubble in mixture；
Step 2, is added micron soft magnetic granules into mixture, the weight ratio of micron soft magnetic granules and mixture be 4:1~Setting time is mixed in 1:1, places into vacuum drying ware and vacuumizes to remove the bubble in mixture；
Step 3 pours mixture obtained in step 2 in the mold of microchannel, places into vacuum drying ware and takes outVacuum is then placed in the baking oven of set temperature to remove bubble removing and toasts setting time；
Mold is put into after impregnating setting time in dehydrated alcohol, removes from the mold the magnetic current with microchannel by step 4Become elastomer；
Magnetic rheology elastic body is blown off with nitrogen, magnetic rheology elastic body is put into plasma cleaning instrument by step 5, is led toIt places up in road；
Step 6, the glass plate that will impregnate setting time in acetone take out, and wash with water and with after being dried with nitrogen, placementIn plasma cleaning instrument, setting time is cleaned；
Step 7 takes out the magnetic rheology elastic body and glass plate of tape channel, carries out and is bonded connection, and is put into set temperatureBaking oven in toast aging setting time after take out.
Wherein, it is 5~10 minutes that setting time, which is mixed,.
Wherein, the set temperature of baking oven is 50~70 DEG C.
Wherein, the setting time toasted in the step 3 is 12~24 hours.
Wherein, the partial size of the micron soft magnetic granules be 2~5 μm, the glass plate with a thickness of 0.2~1mm.
Wherein, baking aging setting time is 1~2 hour.
The present invention also provides a kind of microfluid drives, including the system with the micro-fluidic chip as described in any of the above itemMicro-fluidic chip, substrate, driving motor and the bracket of Preparation Method preparation, the substrate are fastened in the mounting hole of the bracket,The first end face of the substrate is equipped with the groove that adaptation is connect with the driving end of the driving motor, the second end face of the substrateAt least two pairs of group of magnets are installed, the micro-fluidic chip is set on the bracket on the upside of the group of magnets week.
Wherein, each pair of group of magnets includes two magnet monomers that homopolarity is affixed setting, to form gradient magnetic.
Wherein, the second end face of the substrate is equipped with cover board, to the fixation group of magnets.
Wherein, the micro-fluidic chip includes glass plate and is bonded the magnetic rheology elastic body composition of connection with the glass plateChannel, the arrival end in the channel is connected to sample cell.
(3) beneficial effect
The present invention provides a kind of preparation method of micro-fluidic chip and microfluid drive, micro-fluidic chip preparationsMethod is simple, and cost is relatively low, and polydimethylsiloxane prepolymer object transparency is high, and waterproofness and hydrophobicity are good, mix with curing agentMagnetic rheology elastic body can be formed by adding micron soft magnetic granules again afterwards, and magnetic rheology elastic body can generate quickly under external magnetic fieldsThe accurate driving of microfluid is realized in deformation, and structure is simple, at low cost, micro-fluidic chip will not residual liquid, guarantee test and analyzeAs a result accuracy.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of microfluid drive of the embodiment of the present invention；
Fig. 2 is the connection schematic diagram of substrate of the embodiment of the present invention and driving motor；
Fig. 3 is the structural schematic diagram of micro-fluidic chip of the embodiment of the present invention.
In figure, 1: bracket；2: driving motor；3: micro-fluidic chip；4: cover board；5: substrate；6: threaded hole；7: group of magnets；8: main channel；9: subchannel；10: sample cell；11: channel outlet；12: glass plate.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present inventionIn attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment isA part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill peopleMember's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phaseEven ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It canTo be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediaryConnection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete conditionConcrete meaning in invention.
In addition, in the description of the present invention, unless otherwise indicated, " multiple ", " more ", " multiple groups " be meant that two orTwo or more, " several ", " several ", " several groups " are meant that one or more.
The embodiment of the present invention provides a kind of preparation method of micro-fluidic chip, includes the following steps:
Polydimethylsiloxane prepolymer object and curing agent are mixed 5~10 by weight 20:1~15:1 by step 1Minute, after mixing evenly, it is put into vacuum drying ware and vacuumizes 10~30 minutes, remove the bubble in mixture, it is ensured that bubbleRemoval is complete；
Micron soft magnetic granules are added into mixture for step 2, and the partial size of micron soft magnetic granules is 2~5 μm, micron soft magnetismThe weight ratio of particle and mixture is 4:1~1:1, is mixed 5~10 minutes, after mixing evenly, is put into vacuum drying wareIt vacuumizes 10~30 minutes, removes the bubble in mixture, it is ensured that bubble removal is complete；
Step 3 slowly pours mixture obtained in step 2 in the mold of microchannel, places into vacuum drying wareIn vacuumize 10~30 minutes, to remove the bubble in mixture, it is ensured that bubble removal is complete, places into the baking oven of set temperatureMiddle baking setting time, oven temperature are 50~70 DEG C, and the time of baking is 12~24 hours；
Mold is put into dehydrated alcohol and impregnates 0.5~1.5 hour, slowly removes from channel die with micro- by step 4The magnetic rheology elastic body in channel；
Magnetic rheology elastic body is blown off with nitrogen, magnetic rheology elastic body is put into plasma cleaning instrument by step 5, is led toIt places up in road；
Step 6 takes out the glass plate for impregnating setting time in acetone, and soaking time is 12~24 hours, and use is ultrapureAfter water cleans up and uses and be dried with nitrogen, be placed in plasma cleaning instrument, glass plate with a thickness of 0.2~1mm；
Magnetic rheology elastic body with microchannel and glass plate are cleaned 1~2 point by step 7 in plasma cleaning instrumentClock takes out rapidly, after the magnetic rheology elastic body of tape channel and glass plate are gently contacted realization bonding connection, is put into set temperatureBaking oven in toast aging setting time after take out, oven temperature be 50~70 DEG C, baking ageing time be 1~2 hour.
Embodiment 1
By polydimethylsiloxane prepolymer object and curing agent by weight 20:1, it is mixed 10 minutes, stirs evenly, putEnter to be dried in vacuo in ware and handle 30 minutes, removes the bubble in mixture, it is ensured that bubble removal is complete；Grain is added into mixtureThe weight ratio of the micron soft magnetic granules that diameter is 2 μm, micron soft magnetic granules and mixture is 4:1, is mixed 10 minutes, and stirring is equalIt is even, it is put into vacuum drying ware and handles 30 minutes, remove the bubble in mixture, it is ensured that bubble removal is complete；It is mixed by what is obtainedIt closes object slowly to pour in the mold of microchannel, is put into vacuum drying ware and vacuumizes 30 minutes, remove the bubble in mixture, reallyIt is complete to protect bubble removal, places into 70 DEG C of baking oven and toasts 24 hours；Mold after baking is put into dehydrated alcohol and is impregnated1.5 hours, the magnetic rheology elastic body of tape channel is slowly removed from channel die；With nitrogen by the magnetic rheology elastic body of tape channelIt blows off, the magnetic rheology elastic body of tape channel is put into plasma cleaning instrument, channel is placed up；It will impregnate in acetone12 hours glass plates take out, and after being cleaned up with ultrapure water and used and be dried with nitrogen, are placed in plasma cleaning instrument；By bandThe magnetic rheology elastic body and glass plate in channel clean 1 minute in plasma cleaning instrument, take out rapidly, by the magnetic current of tape channelBecome elastomer and glass plate gently contacts after realizing bonding connection, is put into after being toasted aging 2 hours in 70 DEG C of baking oven and takes out.
Embodiment 2
By polydimethylsiloxane prepolymer object and curing agent by weight 17.5:1, it is mixed 7.5 minutes, stirring is equalIt is even, it is put into vacuum drying ware and handles 20 minutes, remove the bubble in mixture, it is ensured that bubble removal is complete；Into mixturePartial size is added as 3.5 μm of micron soft magnetic granules, the weight ratio of micron soft magnetic granules and mixture is 2.5:1, is mixed 8 pointsClock stirs evenly, and is put into vacuum drying ware and vacuumizes 20 minutes, removes the bubble in mixture, it is ensured that bubble removal is complete；Obtained mixture is slowly poured in the mold of microchannel, is put into vacuum drying ware and vacuumizes 20 minutes, remove mixtureIn bubble, it is ensured that bubble removal is complete, places into 60 DEG C of baking oven and toasts 18 hours；Mold after baking is put into anhydrousIt is impregnated 1 hour in ethyl alcohol, the magnetic rheology elastic body of tape channel is slowly removed from channel die；With nitrogen by the magnetic current of tape channelBecome elastomer to blow off, the magnetic rheology elastic body of tape channel is put into plasma cleaning instrument, channel is placed up；It will impregnate16 hours glass plates take out in acetone, after being cleaned up with ultrapure water and used and be dried with nitrogen, are placed on plasma cleanIn instrument；The magnetic rheology elastic body of tape channel and glass plate are cleaned 1.5 minutes in plasma cleaning instrument, taken out rapidly, it willAfter the magnetic rheology elastic body and glass plate of tape channel gently contact realization bonding connection, it is put into 60 DEG C of baking oven and toasts agingIt is taken out after 1.5 hours.
Embodiment 3
By polydimethylsiloxane prepolymer object and curing agent by weight 15:1, it is mixed 5 minutes, stirs evenly, putEnter to be dried in vacuo in ware and handle 10 minutes, removes the bubble in mixture, it is ensured that bubble removal is complete；Grain is added into mixtureThe weight ratio of the micron soft magnetic granules that diameter is 5 μm, micron soft magnetic granules and mixture is 1:1, is mixed 5 minutes, and stirring is equalIt is even, it is put into vacuum drying ware and vacuumizes 10 minutes, remove the bubble in mixture, it is ensured that bubble removal is complete；By what is obtainedMixture slowly pours in the mold of microchannel, is put into vacuum drying ware and vacuumizes 10 minutes, removes the bubble in mixture,Ensure that bubble removal is complete, places into 50 DEG C of baking oven and toast 12 hours；Mold after baking is put into dehydrated alcohol and is soakedBubble 0.5 hour, slowly removes the magnetic rheology elastic body of tape channel from channel die；With nitrogen by the magnetorheological elasticity of tape channelBody is blown off, and the magnetic rheology elastic body of tape channel is put into plasma cleaning instrument, channel is placed up；Acetone will be immersed inIn 24 hours glass plates take out, after being cleaned up with ultrapure water and used and be dried with nitrogen, be placed in plasma cleaning instrument；It willThe magnetic rheology elastic body and glass plate of tape channel clean 2 minutes in plasma cleaning instrument, take out rapidly, by the magnetic of tape channelAfter magnetorheological elastomer and glass plate gently contact realization bonding connection, it is put into after being toasted aging 1 hour in 50 DEG C of baking oven and takes out.
As shown in Figure 1 to Figure 3, the embodiment of the present invention also provides a kind of microfluid drive, including with such as above miniflowMicro-fluidic chip 3, substrate 5, driving motor 2 and the bracket 1 of the preparation method preparation of chip are controlled, substrate 5 is fastened on the peace of bracket 1It fills in hole, specifically, bracket 1, to support entire microfluid drive, the center of the support platform of bracket 1 is equipped with peaceHole is filled, to be clamped substrate 5, guarantees that substrate 5 can rotate in mounting hole.Substrate 5 is specially diameter 60mm, the circle of thickness 10mmShape organic glass.
Further, the first end face of substrate 5 is equipped with the groove that adaptation is connect with the driving end of driving motor 2, realizes baseThe synchronous rotation of plate 5 and driving motor 2.Driving motor 2 is specially 42 serial stepper motors, and bracket 1 is arranged in driving motor 2The lower section of support platform.
Wherein, at least two pairs of group of magnets 7 are installed, each pair of group of magnets 7 is affixed including homopolarity the second end face of substrate 5 equal weekTwo magnet monomers being arranged.Specifically, week is equipped at least two square grooves, each groove in the second end face of substrate 5Interior installation a pair of magnet group 7, group of magnets 7 includes two Neodymium-Iron-Boron super magnet monomers that homopolarity is affixed setting, to generate heightIntensity, high gradient linear magnetic field.In one example, the size of magnet monomer is high 3.2mm, width 3.2mm, long 12.7mm.
Further, the second end face of substrate 5 is equipped with cover board 4, sets in week on cover board 4 there are four through-hole, sets on substrate 5There are four fixed group of magnets 7 by the connection of bolt realization cover board 4 and substrate 5 with the one-to-one threaded hole 6 of through-hole.
Wherein, micro-fluidic chip 3 is set on the bracket 1 of 7 upside of group of magnets, and the magnetic field for guaranteeing that group of magnets 7 generates can act onIn micro-fluidic chip 3.Specifically, micro-fluidic chip 3 includes glass plate 12 and the magnetorheological elasticity for being bonded connection with glass plate 12The channel that body is constituted, channel by main channel 8 and positioned at 8 both ends of main channel and two subchannels 9 in communication, subchannel 9Arrival end is connected to sample cell 10, and sample cell 10 is to be added dropwise stored sample, by the wriggling in magnetic field in channel, sample warpChannel outlet 11 flows out.
The process flow of microfluid drive of the embodiment of the present invention is as follows:
Micro-fluidic chip 3 is seated on bracket 1, appropriate amount of sample is drawn with liquid-transfering gun and is added in sample cell, due to sampleProduct pond opening is larger, therefore can store a large amount of samples, and later on driving motor switch, driving motor drives coupled basePlate quickly rotates (rotation speed: 60rpm~120rpm), puts since two pieces of super magnet homopolarities are opposite, in two blocks of magnetBetween generate linear superpower high-gradient magnetic field and rotate；The magnetic rheology elastic body of main channel occurs for superpower rotating excitation fieldPeriodic deformation, deformation will cause main channel to generate mobile temporary enclosed chamber, and then pass through the sample in sample cell 11Subchannel and main channel are extruded to exit, and are used for the sample supply of duration.
The preparation method and microfluid drive of a kind of micro-fluidic chip provided by the invention, micro-fluidic chip preparationMethod is simple, and cost is relatively low, and polydimethylsiloxane prepolymer object transparency is high, and waterproofness and hydrophobicity are good, mix with curing agentMagnetic rheology elastic body can be formed by adding micron soft magnetic granules again afterwards；Magnetic rheology elastic body can generate quickly under external magnetic fieldsThe accurate driving of microfluid is realized in deformation, and structure is simple, at low cost, micro-fluidic chip will not residual liquid, guarantee test and analyzeAs a result accuracy.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the inventionWithin mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.