A kind of high-effective microorganism system for pretreating sampleTechnical field
The invention belongs to the field of medical instrument technology, in particular to a kind of high-effective microorganism system for pretreating sample.
Background technique
Clinical microbiology laboratory technique be the fields such as medical institutions' medical diagnosis on disease, antibiotic reasonable employment, hospital infection control notThe helper that can or lack.Microorganism sample pre-treatment is the important link in examining, and heavy workload spends the time long, processing resultAlso the accuracy of subsequent all detections is directly affected.Just because of this, how efficient high texture handles microorganism sample, becomes raisingThe problem of Micro biological Tests speed, efficiency and quality.
The culture medium streak inoculation work of Microbiological Lab of medical institutions of the country relies primarily on manual operations at present, worksFor personnel due to the close contact with clinical samples in seeded process, itself also faces the risk of biological infection.In addition, by handOperation is influenced very big by human factor, and random, the nonstandard parasexuality of operation and error etc. frequently result in sample streak inoculation notWhen and cause the mistakes of cultivation results.Therefore, a kind of full automatic micro-biological samples processing system with standard processing capacitySeem to market and especially needs.
Summary of the invention
The present invention is in view of the above-mentioned problems, spy proposes a kind of high-effective microorganism system for pretreating sample.
Technical scheme is as follows:
A kind of high-effective microorganism system for pretreating sample, comprising: the sample bit, sample tube clamping with shaking table and upper cover dress of uncappingSet, disinfector, plane motion classification inoculation apparatus, untreated plate position in storehouse, processed plate position in storehouse, plate crawl and uncapBuckle closure device, vaccination station, automatic labeling device, culture dish return bin device.
Wherein, sample bit includes the shaking table in base plate bottom installation, has a certain number of pores and each on the shaking tableThe pore size and sample tube of pore match for placing sample tube.
Sample to be examined pipe is placed in the pore of shaking table, after instrument starting, the sample in sample tube shakes with shaking table,The homogenize process that can promote sample improves the recall rate of sample.
Sample tube clamping and capping device of uncapping include the mechanical arm positioned at sample bit side, which is with X3 shaft mechanical arms of axis, Y-axis and Z-direction transmission, be equipped on mechanical arm realization sample tube is clamped, uncap and onCover the collet of operation；Above-mentioned sample tube clamping and capping device of uncapping further include having to be located at sampling position and for fixed sample pipeSample pipe clip.
After instrument starting, can collet by 3 shaft mechanical arms and thereon realize the clamping and transhipment of sample tube, i.e., byCollet grabs the sample tube (position of collet crawl is the lid on sample tube top) on sample bit, and passes through mechanical arm for samplePipe is transferred to sampling position by sample bit, then is clamped fixation to sample tube by the sample pipe clip on sampling position；Later, machineCollet on tool arm is rotated counterclockwise to unscrew the lid of sample tube, is moved to side by mechanical arm after unscrewing；Knot to be sampledShu Hou, mechanical arm control collet are again moved into above sample tube, are moved downward, when lid is pressed against the nozzle of sample tubeWhen, rotating electric machine drive collet rotation lid to be screwed, later mechanical arm control collet move upwards with sample tube lidIt is detached from, completes upper cover.After upper cover, control sample pipe clip unclamps, and sample tube falls into sample tube working area.
As a kind of preferred structure of collet in above scheme, above-mentioned collet is to be mounted on mechanical arm by rotating electric machineOn, the built-in spring that can be fastened on sample tube lid is provided in the tube wall of collet.When clamping: mechanical arm controls collet and movesIt moves above sample tube and moves down, the lid of sample tube can be packed into the built-in spring of collet by pressure, realizedClamping to sample tube；When uncapping: being rotated, sample tube lid can be unscrewed counterclockwise by controlling rotating electric machine；When upper cover:Lid is pressed into the nozzle of sample tube by mechanical arm control collet, and rotating electric machine, which rotates clockwise, screws lid, mechanical laterArm control collet moves up, and is detached from spring and sample tube lid using upward pulling force, completes upper cover.
As a kind of preferred structure of sample pipe clip in above scheme, above-mentioned sample pipe clip is pressed from both sides by two panelsThe clip of piece composition is constituted, and two intermediate plates are separately mounted on small-sized transmission device motor-driven.
Disinfector is high-temperature electromagnetic sterilizer.
Plane motion classification inoculation apparatus includes the vertical transport (vertical electric pole) being connected with substrate, vertical transportUpper end horizontal rotation support base motor-driven is installed, rotate horizontally and horizontal transport mechanism, water be installed on support baseThe fixed device of oese is installed in flat transport mechanism, rotating electric machine is set in the fixed device of oese, under rotating electric machine driveRotation adjustment inoculation ring position.
When work: oese is inserted into high-temperature electromagnetic sterilizer the sterilization that carries out disinfection by vertical transport, to be sterilized to killAfter bacterium, vertical transport moves upwards extraction oese.By rotating horizontally the rotation of support base, oese is rotatedTo sample tube of having uncapped, moves down and dip sample.It rotates after the completion of sampling to vaccination station.
The bottom of untreated plate (culture dish) position in storehouse is connected with rotating electric machine, and thereon circle distribution have it is a certain number ofCulture dish storage position, the bottom of culture dish storage position have a circle card edge with support culture dish；It is set in the side close to vaccination stationIt is equipped with the culture dish delivery device being located under culture dish storage position bottom surface, the culture dish delivery device is by vertical transport and itsTop diameter is less than culture dish storage position bottom card and forms along the culture dish supporting spring of internal diameter.
When work: untreated culture dish position in storehouse rotates under the rotating electric machine effect of its bottom, the culture dish that will be inoculated withStorage position is rotated to culture dish delivery device position.Vertical transport below culture dish delivery device moves upwards, and hangs downThe culture dish that culture dish supporting spring at the top of straight transport mechanism withstands on culture dish storage position, which moves up to release in culture dish, to be trainedSupport ware storage position.
Plate crawl and buckle closure device of uncapping are located at the outside of untreated culture dish position in storehouse, include to be connected on substrateVertical transport (vertical electric pole) is equipped with horizontal rotation support base motor-driven on the top of vertical transport,It rotates horizontally and horizontal transport mechanism is installed on support base, one end of horizontal transport mechanism is equipped with electric pawl, the point of application of electric pawlRunner is installed to realize automatic centering during grabbing culture dish or culture ware lid in position.Plate crawl and buckle closure of uncappingDevice rotates electric pawl to untreated culture dish position in storehouse top, the culture dish that clamping has been pushed out to vaccination station.
Plate is uncapped buckle closure: electric pawl crawl culture dish lower part simultaneously takes culture dish in vaccination station to, and later, electric pawl moves up oneCulture ware lid is set and is grabbed in positioning, and electric pawl continues to move up to remove culture ware lid, and the rotation by rotating horizontally support base comeCulture ware lid is rotated to side；It rotates after crossing, then by the culture ware lid on electric pawl to culture dish, moves down buttonLid.
Vaccination station: culture dish vaccination station lower part is equipped with rotating electric machine, and integral installation is on a vaccination station support frame.InoculationPlatform support frame is mounted on horizontal conveyor, and culture medium inoculated platform is moved along horizontal conveyor driven by the motor.
When work: culture dish is placed in after vaccination station uncaps, and inoculating mechanism arm is rotated to vaccination station, the fixed dress of oeseRotation adjustment inoculation ring position is set, favours oese above culture medium.When inoculation, inoculating mechanism arm is moved along anterior-posterior horizontalIt is dynamic, move oese on culture medium along Y-axis；It is round-trip under the horizontal conveyor effect of vaccination station thereunder or so to moveIt is dynamic, move culture dish along X-axis, the streak inoculation of sample is realized in the superposition of both sides' motion profile.When the complete region of streak inoculationAfterwards, vaccination station makes culture medium change an angle, then realizes separately according still further to above-mentioned streak inoculation mode under rotating electric machine effectThe streak inoculation in one region.By above-mentioned vaccination ways can realize 3 rides of micro-biological samples preprocessing system, 4 rides,The different hatched manner such as rich word scribing line and spiral scribing line.
Automatic labeling device includes a pedestal, installs a rotating electric machine, one rotation of rotating electric machine one end connection on the baseTurn bracket, rotating electric machine rotation is able to drive runing rest and rotates in perpendicular, and the lower section in the front end of runing rest is solidSurely it is connected with label absorption panel, can be bolted to connection, label adsorbs panel and is equipped with suction hole, and label adsorbs under panelLabel limiting device is placed by side, wherein the suction end of a vacuum pump is connect with label absorption panel, the outlet side of vacuum pump and markSign limiting device connection.
The work of automatic labelling apparatus approximately as: firstly, rotating electric machine drive runing rest it is suitable in vertical planeHour hands rotation, runing rest drive label absorption panel rotation, when runing rest top is rotated to horizontality, stopping simultaneouslyRotation, at this moment the label absorption panel on runing rest is located just at printer paper outlet position top position, label limit dressIt is placed in the underface of label absorption panel, the i.e. lower section of printer paper outlet position, the label printed from printerSmooth viscose is face-down up, at this moment opens vacuum pump, and label limiting device connects vacuum pump outlet side up towards under labelSurface is blown, and vacuum pump suction end, which generates negative pressure, is attracted to label on label absorption panel, and at this moment rotating electric machine is opened againOpposite direction rotation is opened, runing rest is driven to rotate counterclockwise in vertical plane, when runing rest top is rotated to horizontality,Rotate 180 ° counterclockwise i.e. in vertical plane, the label viscose adsorbed at this moment label absorption panel is face-up, and culture dish is sentIt to label, is located just at the bottom of culture dish above label, then clamps culture dish and complete labeling movement downwards.
Culture dish returns bin device: being equipped with culture dish transmission device in the side in processed culture dish storehouse, which passesThe bottom surface for sending the height of device to be lower than processed culture dish storehouse；Culture dish is installed in one end of culture dish transmission device to pushPlate is equipped with culture dish driving means in one end close to processed culture dish storehouse, and the structure of culture dish driving means is the same as above-mentionedCulture dish delivery device it is identical.Processed culture dish chamber structure is substantially the same with untreated culture dish position in storehouse, is distinguished as having locatedThe storage position bottom surface in reason culture dish storehouse is equipped with symmetrical two panels culture dish supporting plate, the outside of culture dish supporting plate and storage position hingeIt connects, inside can only be rotated up.The diameter of culture dish supporting spring is less than the distance between two symmetrical supporting plates on culture dish driving means,The position of culture dish driving means and the storage position bottom surface gap in processed culture dish storehouse coincide, culture dish supporting spring with it is processedThe distance between orlop face is more than or equal to the thickness of a culture dish.When work: after the completion of culture dish labeling, electric pawl crawl trainingIt supports ware and is put on the culture dish transmission device of processed culture dish storehouse side, then culture dish pushing plate shifts culture dish ontoOn the culture dish supporting spring for handling culture dish orlop portion, culture dish supporting spring moves upwards under the action of moving vertically electric pole, will trainAfter supporting ware support to two panels culture dish supporting plate top, the state of two panels culture dish supporting plate maintenance level position is filled when culture dish pushesDuring the vertical movement electric pole set moves downward, culture dish can be held by two panels culture dish supporting plate.
The beneficial effects of the present invention are: high-effective microorganism system for pretreating sample is using mechanical arm instead of traditional-handwork methodSample process and streak inoculation carry out screw capping to micro-biological samples automatically, barcode scanning, patch code, carry, uncap, upper cover, streak inoculationEqual sequence of operations, can greatly ensure the bio-safety of microorganism staff, improve work efficiency simultaneously.
Detailed description of the invention
Attached drawing 1 is schematic perspective view of the invention；
Attached drawing 2 is another visual angle of present invention ground schematic perspective view；
Attached drawing 3 is overlooking surface structural schematic diagram of the invention；
Attached drawing 4 is the structural schematic diagram of mechanical arm and collet in the present invention；
Attached drawing 5 is the structural schematic diagram of sample pipe clip in the present invention；
Attached drawing 6 is the structural schematic diagram of untreated culture dish position in storehouse in the present invention；
Attached drawing 7 is the structural schematic diagram of electric pawl in the present invention；
Attached drawing 8 is the structural schematic diagram of vaccination station in the present invention；
Attached drawing 9 is the structural schematic diagram of oese in the present invention；
Attached drawing 10 is the structural schematic diagram of automatic labeling device in the present invention；
Attached drawing 11 is the structural schematic diagram of culture dish transmission device in the present invention；
Attached drawing 12 is the structural schematic diagram of processed plate position in storehouse in the present invention；
Attached drawing 13 is the structural schematic diagram of collet；
In figure, 1-1 shaking table, 1-2 pore, 2-1 mechanical arm, 2-2 collet, 2-2-1 tube wall, 2-2-2 spring, 2-3 sample tube fixedFolder, 3 high-temperature electromagnetic sterilizers, 4-1 horizontal transport mechanism, the fixed device of 4-2 oese, 4-3 rotating electric machine, 4-4 oese, 5Untreated culture dish position in storehouse, 5-1 culture dish storage position, 6 vaccination stations, 6-1 horizontal conveyor, 7-1 electricity pawl, 7-2 runner, 8 certainlyDynamic device for labeling, 8-2 bar code printer, 9 culture dish transmission devices, 10 processed culture dish freight spaces.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Embodiment
As shown in the figure, which includes the sample bit with shaking table, sample tube clamping and capping device of uncapping, disinfectionBactericidal unit, plane motion classification inoculation apparatus, untreated plate position in storehouse, processed plate position in storehouse, plate crawl and buckle closure dress of uncappingIt sets, vaccination station, automatic labeling device, culture dish return bin device.
Wherein, sample bit includes the shaking table 1-1 in base plate bottom installation, has a certain number of pore 1- on the shaking table2 and the pore size and sample tube of each pore match with for placing sample tube.
Sample to be examined pipe is placed in the pore of shaking table, after instrument starting, the sample in sample tube shakes with shaking table,The homogenize process that can promote sample improves the recall rate of sample.
Sample tube clamping and capping device of uncapping include the mechanical arm 2-1 positioned at sample bit side, which is toolHave 3 shaft mechanical arms of X-axis, Y-axis and Z-direction transmission, be equipped on mechanical arm realization sample tube is clamped, uncap andThe collet 2-2 of upper cover operation；Above-mentioned sample tube clamping and capping device of uncapping further include having to be located at sampling position and for fixingThe sample pipe clip 2-3 of sample tube.
After instrument starting, can collet by 3 shaft mechanical arms and thereon realize the clamping and transhipment of sample tube, i.e., byCollet grabs the sample tube (position of collet crawl is the lid on sample tube top) on sample bit, and passes through mechanical arm for samplePipe is transferred to sampling position by sample bit, then is clamped fixation to sample tube by the sample pipe clip on sampling position；Later, machineCollet on tool arm is rotated counterclockwise to unscrew the lid of sample tube, is moved to side by mechanical arm after unscrewing；Knot to be sampledShu Hou, mechanical arm control collet are again moved into above sample tube, are moved downward, when lid is pressed against the nozzle of sample tubeWhen, rotating electric machine drive collet rotation lid to be screwed, later mechanical arm control collet move upwards with sample tube lidIt is detached from, completes upper cover.After upper cover, control sample pipe clip unclamps, and sample tube falls into sample tube working area.
As a kind of preferred structure of collet in above scheme, above-mentioned collet is to be mounted on mechanical arm by rotating electric machineOn, the built-in spring 2-2-2 that can be fastened on sample tube lid is provided in the tube wall 2-2-1 of collet.When clamping: mechanical armControl collet is moved to above sample tube and moves down, and the lid of sample tube can be packed into the built-in bullet of collet by pressureIn spring, the clamping to sample tube is realized；When uncapping: being rotated, sample tube lid can be revolved counterclockwise by controlling rotating electric machineIt opens；When upper cover: lid is pressed into the nozzle of sample tube by mechanical arm control collet, and rotating electric machine, which rotates clockwise, revolves lidTightly, the collet of mechanical arm control later moves up, and is detached from spring and sample tube lid using upward pulling force, completes upper cover.
As a kind of preferred structure of sample pipe clip in above scheme, above-mentioned sample pipe clip is pressed from both sides by two panelsThe clip of piece composition is constituted, and two intermediate plates are separately mounted on small-sized transmission device motor-driven.
Disinfector is high-temperature electromagnetic sterilizer 3.
Plane motion classification inoculation apparatus includes the vertical transport I (vertical electric pole) being connected with substrate, vertical conveyorThe upper end of structure I is equipped with horizontal rotation support base motor-driven, rotates horizontally and is equipped with horizontal transport mechanism on support base4-1 is equipped with the fixed device 4-2 of oese in horizontal transport mechanism, sets rotating electric machine 4-3 in the fixed device of oese, revolvingRotating motor drives the position backspin modulation whole oese 4-4.
When work: oese is inserted into high-temperature electromagnetic sterilizer the sterilization that carries out disinfection by vertical transport I, to be sterilized to killAfter bacterium, vertical transport moves upwards extraction oese.By rotating horizontally the rotation of support base, oese is rotatedTo sample tube of having uncapped, moves down and dip sample.It rotates after the completion of sampling to vaccination station.
The bottom of untreated plate (culture dish) position in storehouse 5 is connected with rotating electric machine, and circle distribution has certain amount thereonCulture dish store in a warehouse position 5-1, the store in a warehouse bottom of position of culture dish has a circle card along with support culture dish；Close to the one of vaccination stationSide is provided with the culture dish delivery device stored in a warehouse under the bottom surface of position positioned at culture dish, and the culture dish delivery device is by vertical transportII (vertical electric pole) and its top diameter are less than culture dish storage position bottom card and form along the culture dish supporting spring of internal diameter.
When work: untreated culture dish position in storehouse rotates under the rotating electric machine effect of its bottom, the culture dish that will be inoculated withStorage position is rotated to culture dish delivery device position.Vertical transport II below culture dish delivery device moves upwards,The culture dish that the culture dish supporting spring at II top of vertical transport withstands on culture dish storage position, which moves up to, pushes away culture dishCulture dish storage position out.
Plate crawl and buckle closure device of uncapping are located at the outside of untreated culture dish position in storehouse, include to be connected on substrateVertical transport III (vertical electric pole) is equipped with horizontal rotation support motor-driven on the top of vertical transport IIISeat rotates horizontally and is equipped with horizontal transport mechanism on support base, and one end of horizontal transport mechanism is equipped with electric pawl 7-1, electric pawlRunner 7-2 is installed to realize automatic centering during grabbing culture dish or culture ware lid in point of application position.Plate crawlAnd buckle closure device of uncapping rotates electric pawl to untreated culture dish position in storehouse top, the culture dish that clamping has been pushed out to vaccination station.
Plate is uncapped buckle closure: electric pawl crawl culture dish lower part simultaneously takes culture dish in vaccination station to, and later, electric pawl moves up oneCulture ware lid is set and is grabbed in positioning, and electric pawl continues to move up to remove culture ware lid, and the rotation by rotating horizontally support base comeCulture ware lid is rotated to side；It rotates after crossing, then by the culture ware lid on electric pawl to culture dish, moves down buttonLid.
Vaccination station: 6 lower part of culture dish vaccination station is equipped with rotating electric machine, and integral installation is on a vaccination station support frame.It connectsKind platform support frame is mounted on horizontal conveyor 6-1, and culture dish vaccination station is moved along horizontal conveyor driven by the motorIt is dynamic.
When work: culture dish is placed in after vaccination station uncaps, and plane motion classification inoculation apparatus is rotated to vaccination station, oeseFixed device rotation adjustment inoculation ring position, favours oese above culture medium.When inoculation, control plane motion inoculation dressIt sets along horizontal shifting, moves oese on culture medium along Y-axis；The horizontal conveyor effect of vaccination station thereunderLower left and right moves back and forth, and moves culture dish along X-axis, and the streak inoculation of sample is realized in the superposition of both sides' motion profile.When scribing line connectsAfter finishing a region, vaccination station makes culture medium change an angle, then connects according still further to above-mentioned scribing line under rotating electric machine effectKind mode realizes the streak inoculation in another region.3rd area of micro-biological samples preprocessing system can be realized by above-mentioned vaccination waysThe different hatched manners such as scribing line, the scribing line of 4 rides, rich word and spiral scribing line.
Automatic labeling device 8 includes a pedestal, installs a rotating electric machine, one rotation of rotating electric machine one end connection on the baseTurn bracket, rotating electric machine rotation is able to drive runing rest and rotates in perpendicular, and the lower section in the front end of runing rest is solidSurely it is connected with label absorption panel, can be bolted to connection, label adsorbs panel and is equipped with suction hole, and label adsorbs under panelLabel limiting device is placed by side, wherein the suction end of a vacuum pump is connect with label absorption panel, the outlet side of vacuum pump and markSign limiting device connection.
The work of automatic labelling apparatus approximately as: firstly, rotating electric machine drive runing rest it is suitable in vertical planeHour hands rotation, runing rest drive label absorption panel rotation, when runing rest top is rotated to horizontality, stopping simultaneouslyRotation, at this moment the label absorption panel on runing rest is located just at bar code printer 8-2 exit slot position top position, labelLimiting device is placed in the underface of label absorption panel, the i.e. lower section of printer paper outlet position, prints from printerLabel it is smooth viscose is face-down up, at this moment open vacuum pump, label limiting device connect vacuum pump outlet side up towardsLabel lower surface is blown, and vacuum pump suction end, which generates negative pressure, is attracted to label on label absorption panel, at this moment rotating electric machineIt is again turned on opposite direction rotation, drives runing rest to rotate counterclockwise in vertical plane, when runing rest top is rotated to levelWhen state, i.e., rotate 180 ° counterclockwise in vertical plane, the label viscose adsorbed at this moment label absorption panel is face-up, will trainFeeding ware is sent to label, is located just at the bottom of culture dish above label, is then clamped culture dish and is completed labeling movement downwards.
Culture dish returns bin device: being equipped with culture dish transmission device 9 in the side in processed culture dish storehouse 10, the culture dishThe height of transmission device is lower than the bottom surface in processed culture dish storehouse；Culture dish is installed in one end of culture dish transmission device to pushPlate is equipped with culture dish driving means in one end close to processed culture dish storehouse, and the structure of culture dish driving means is the same as above-mentionedCulture dish delivery device it is identical.Processed culture dish chamber structure is substantially the same with untreated culture dish position in storehouse, is distinguished as having locatedThe storage position bottom surface in reason culture dish storehouse is equipped with symmetrical two panels culture dish supporting plate, the outside of culture dish supporting plate and storage position hingeIt connects, inside can only be rotated up.The diameter of culture dish supporting spring is less than the distance between two symmetrical supporting plates on culture dish driving means,The position of culture dish driving means and the storage position bottom surface gap in processed culture dish storehouse coincide, culture dish supporting spring with it is processedThe distance between orlop face is more than or equal to the thickness of a culture dish.When work: after the completion of culture dish labeling, electric pawl crawl trainingIt supports ware and is put on the culture dish transmission device of processed culture dish storehouse side, then culture dish pushing plate shifts culture dish ontoOn the culture dish supporting spring for handling culture dish orlop portion, culture dish supporting spring moves upwards under the action of moving vertically electric pole, will trainAfter supporting ware support to two panels culture dish supporting plate top, the state of two panels culture dish supporting plate maintenance level position is filled when culture dish pushesDuring the vertical movement electric pole set moves downward, culture dish can be held by two panels culture dish supporting plate.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared withGood embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the inventionArt scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at thisIn the scope of the claims of invention.