Stem cell culture perfusing deviceTechnical field
The present invention relates to a kind of biological culture field devices, especially a kind of stem cell for improving culture efficiency cultivates perfusionDevice.
Background technique
Stem cell (SCs) has on cell therapy, organizational engineering and regenerative medicine and drug and biotechnology applicationsHuge prospect.Stem cell has the ability of self-renewing, and can be divided into specific cell type and (depend on stem cellSeparation source).However, stem cell application clinically needs to use the stem cell of high quality and high quantity, this just needs to doThe extensive expansion of cell, then effectively and be uniformly divided into functional derivatives.Traditional cell maintains and expansion relies onUnder two dimension (2-D) culture technique using plastic culture plate and xenogenesis culture medium, this method, cell expansion limitation,And after Long Term Passages, cell often loses clone and differentiation capability.Recently, the new method of expansion of stem cells emphasizes three-dimensional (3-D)Cell is grown to imitate vivo environment.The literature in the recent period using sphere involved in two and three dimensions technology culture stem cell,The latest developments of biomaterial and bioreactor provide a comprehensive summary.
Summary of the invention
In order to overcome deficiency existing for existing technology, the present invention provides a kind of stem cell culture perfusing device, this is dry thinBorn of the same parents' culture perfusion device is practical, and easy to operate, safety is good, and cleannes are high, easy to use, high reliablity.
The technical solution adopted by the present invention to solve the technical problems is: the present invention includes beam pipe, perfusion bottle, perfusion machineStructure, lubricator, cleaning mechanism, pipe fitting, casing head, snorkel, sewage pipe, casing, oil pipe, gas injection joint, connector, sprayHead, continuous tube passage, interior annulus access.
Including perfusion bottle, perfusion mechanism, cleaning mechanism, perfusion mechanism is installed under perfusion bottle, is equipped under perfusion mechanismLubricator is equipped with cleaning mechanism under lubricator, and pipe fitting is equipped under cleaning mechanism, and pipe fitting connects snorkel, pipe fittingUnder casing head is installed, described sleeve pipe head connects sewage pipe, and casing is connected under casing head, includes beam pipe, beam pipe in casingConnection perfusion bottle, perfusion mechanism, lubricator, cleaning mechanism, pipe fitting, casing head, beam pipe are connected to downwards connector upwards, evenThe external spray head of device is connect, further includes oil pipe, gas injection joint in casing, gas injection joint upper end is connected to snorkel, and the note in casingFlow tube, oil pipe are equipped with continuous tube passage, interior annulus access between gas injection joint.
The beam pipe is connected with culture dish, and the piston is arranged in outlet tube inner surface.
Perfusion bottle end face offers perfusion hole.
The spray head is specially atomizer, and beam pipe internal surface is equipped with annular-heating piece.
The invention has the advantages that practical, easy to operate, safety is good, and cleannes are high, easy to use, reliablyProperty it is high.
Detailed description of the invention
Fig. 1 is the structural map of stem cell culture perfusing device embodiment.
Fig. 2 is the structural map of spray head embodiment.
In figure: 1, beam pipe；2, perfusion bottle；3, perfusion mechanism；4, lubricator；5, cleaning mechanism；6, pipe fitting；7, it coversTube head；8, snorkel；9, sewage pipe；10, casing；11, oil pipe；12, gas injection joint；13, connector；14, spray head；15, continuousTube passage；16, interior annulus access.
Specific embodiment
Present invention will be further explained below with reference to the attached drawings and examples.
In the embodiment shown in fig. 1, the present invention includes beam pipe 1, perfusion bottle 2, perfusion mechanism 3, lubricator 4, cleanerStructure 5, pipe fitting 6, casing head 7, snorkel 8, sewage pipe 9, casing 10, oil pipe 11, gas injection joint 12, connector 13, spray head 14,Continuous tube passage 15, interior annulus access 16.
Including perfusion bottle 2, perfusion mechanism 3, cleaning mechanism 5, perfusion mechanism 3 is installed under perfusion bottle 2, under perfusion mechanism 3Lubricator 4 is installed, cleaning mechanism 5 is installed under lubricator 4, pipe fitting 6 is installed under cleaning mechanism 5, pipe fitting 6 is connected logicalTracheae 8, casing head 7 is equipped under pipe fitting 6, and described sleeve pipe head 7 connects sewage pipe 9, the lower connection casing 10 of casing head 7, casingIt include beam pipe 1 in 10, beam pipe 1 is connected to perfusion bottle 2, perfusion mechanism 3, lubricator 4, cleaning mechanism 5, pipe fitting 6, set upwardsTube head 7, beam pipe 1 connection connector 13, the external spray head 14 of connector 13 downwards further include that oil pipe 11, gas injection connect in casing 10First 12,12 upper end of gas injection joint is connected to snorkel 8, and sets between beam pipe 1 in casing 10, oil pipe 11, gas injection joint 12There are continuous tube passage 15, interior annulus access 16.The beam pipe 1 is connected with culture dish, and the piston is arranged in outlet tubeSurface.2 end face of perfusion bottle offers perfusion hole.The spray head 14 is specially atomizer, and 1 inner surface of beam pipe is installedThere is annular-heating piece.
When it is implemented, being connected to perfusion bottle 2, perfusion mechanism 3, lubricator 4, cleaning mechanism 5, pipe upwards by beam pipe 1Connector 6, casing head 7, connection connector 13, the external spray head 14 of connector 13, each mechanism synergistic effect fill beam pipe 1 downwardsFlow velocity rate is safely controllable, and perfusion is high-efficient.
In specific implementation, as shown in Fig. 2, spray head 14 includes supervisor 1a, the upper end supervisor 1a is equipped with pedestal 2a, describedThe front end pedestal 2a is equipped with revolving part 3a, and the rear end the pedestal 2a is equipped with multiple carries propulsion chamber 4a, before the carry promotes chamber 4aEnd is connected with strength spray orifice 5a, and the strength spray orifice 5a is exported to be arranged outside the pedestal 2a, and the carry promotes the front end chamber 4a to leadPushing ram 6a out, the revolving part 3a output end export a vertical bull stick 7a.
As known by the technical knowledge, the present invention can pass through the embodiment party of other essence without departing from its spirit or essential featureCase is realized.Embodiment disclosed above, in all respects are merely illustrative, not the only.It is all thisIt is included in the invention in invention scope or in the change being equal in the scope of the present invention.