The detection device of biological sample quick detection kit and adaptationTechnical field
The inspection of of the invention and a kind of fluorescence detection device more particularly to a kind of biological sample quick detection kit and adaptationSurvey device.
Background technique
Common detection of biological samples method at present, as latex enhances turbidimetric analysis, chemiluminescence analysis, ELISAAnd Time-resolved fluorescence assay, or it need to be equipped with large-scale instrument, high to place, equipment requirement, testing cost is expensive；Or the degree of automationNot high, trivial operations, the reaction time is long, and operator's profession requires high.
Wherein fluorescence immunoassay detection technique has many advantages, such as that specificity is strong, high sensitivity, practicability are good, therefore it is used forMeasure the very low bioactive compound of content, such as protein (enzyme, acceptor, antibody), hormone (steroid, first shapeGlandular hormone, phthalein hormone), drug and microorganism etc..But sample is being dripped before Test paper, needs more complicated pre-treatment,It is unfavorable for the processing of amateur testing staff.
Summary of the invention
The object of the present invention is to provide a kind of biological sample quick detection kit and the detection devices of adaptation.
According to an aspect of the present invention, the detection device of biological sample quick detection kit and adaptation includes detectionInstrument main body, detection kit and the detection device being adapted to detection kit, detector main body include detection probe, detection probeComprising ultraviolet lasing device, fluorescence signal acquisition device, be successively arranged from top to bottom in detection kit probe tube, screen pipe andTest paper, detection kit shell left side outer wall are equipped with detection hole, and detection kit shell outer right wall is equipped with guiding fixed groove,Detection device inner sidewall is equipped with the corresponding fixed ball of guiding fixed groove.Sampling and test paper are completed in detection kit as a result,After detection, it will test and be fixedly connected in kit insertion detection device with detection device, will test probe and be inserted to across detection holeIn detection kit, test paper is detected.
On the other hand, detection kit housing top surface is equipped with the sample holes cooperated with the probe tube, detection kit shellBody sidewall is additionally provided with the first fixing clamp for fixing the probe tube.After probe tube completes sampling as a result, inserted by sample holesIt puts to detection kit, then is fixed probe tube by the first fixing clamp.
On the other hand, probe tube includes the first fixing clamp and the sampler space from top to bottom, and the first fixing clamp is top openingHemi-closure space structure, the sampler space is the hemi-closure space structure of bottom opening, and the first fixing clamp bottom is logical equipped with screw threadRoad, screw channel, which is twined, to be communicated at the top of sampler space outer wall, screw channel with the first fixing clamp, the closing of screw channel bottom,Screw channel is equipped with several through-holes communicated with the sampler space with the inner wall that the sampler space is in contact.The first fixing clamp is logical as a result,Crossing screw channel, through-hole can communicate with the sampler space.
On the other hand, middle part is equipped with liquid storage tank in the first fixing clamp, is equipped with piercing needle, puncture needle at the top of the first fixing clampFirst side is equipped with guide post, is equipped with the guide through hole cooperated with guide post by sample holes.Piercing needle is pressed as a result, is allowed to edgeGuide post direction moves downward, after puncturing liquid storage tank, downward liquid flow in liquid storage tank, threadingly channel, by through-hole to takingSample is rinsed in sample space.
On the other hand, detection kit housing sidewall is additionally provided with the second fixing clamp for fixing screen pipe.It can lead to as a result,The second fixing clamp is crossed to fix screen pipe.
On the other hand, screen pipe is upper and lower opening structure, is equipped with glass fibre membrane in the middle part of screen pipe.As a result, via liquid storageSample liquid in pond after liquid wash can be filtered again to reach by glass fibre membrane and mix sample liquid indirectlyPurpose.
On the other hand, detection reagent outer box wall, which is sticked, bar code, and detection device inner wall is equipped with item corresponding with bar codeShape code identifier.Barcode recognizer reads the bar code in detection kit as a result, to guide detector starting and the inspectionThe matched detection project program of test agent box.
On the other hand, detection kit top and bottom outer wall is respectively equipped with the first fixed cynapse and the second fixed cynapse,Detection device inner wall is equipped with to be fixed shrinkage pool and second and fixes with the first fixed cynapse and the second fixed cynapse corresponding first respectivelyShrinkage pool.Thus, it is possible to by the first fixed cynapse, the second fixed cynapse, the first fixed shrinkage pool and the second fixed shrinkage pool reach intoThe purpose of one step control detection kit and detection device relative position.
On the other hand, detection device inner sidewall bottom is equipped with guide fixing plate, in guide fixing plate side and detection deviceSide wall is fixedly connected, and fixed ball is set to the other side of guide fixing plate by spring.
On the other hand, detection device is outer, detector main body is equipped with spring control switch.After the completion of detecting as a result, pass throughSpring control switch will test kit and fixed ball and separate, to take out detection kit.
On the other hand, test paper platform is equipped with below test paper, test paper and test paper platform constitute detection card, detect in some embodimentsCard can be removed for detecting.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of detection kit in the present invention；
Fig. 2 is the external structure schematic diagram of detection kit in the present invention；
Fig. 3 is the structural schematic diagram of the partial enlargement of probe tube in the present invention；
Fig. 4 is the structural schematic diagram of detection device in the present invention；
Fig. 5 is the structural schematic diagram of guide fixing plate in the present invention；
Fig. 6 is the structural schematic diagram of the test paper referred in background of invention；
Fig. 7 is the structural schematic diagram popped one's head in the present invention；
Fig. 8 is the external structure schematic diagram of detector main body in the present invention.
Specific embodiment
The invention will now be described in further detail with reference to the accompanying drawings.
Embodiment 1
FIG. 1 to FIG. 8 schematically shows the detection of biological sample quick detection kit and adaptation according to the present inventionThe structural representation of device.As shown in Fig. 1,6 and 7, the detection device of biological sample quick detection kit and adaptation includes detectionInstrument main body 1, detection kit 2 and the detection device 3 being adapted to detection kit 2, detector main body 1 include detection probe 101,Detection probe 101 includes ultraviolet lasing device 1011, fluorescence signal acquisition device 1012, in detection kit 2 from top to bottom according toSecondary to be equipped with probe tube 4, screen pipe 5 and test paper 6, outer wall is equipped with detection hole 7,2 shell of detection kit on the left of 2 shell of detection kitBody outer right wall is equipped with guiding fixed groove 8, and 3 inner sidewall of detection device is equipped with the corresponding fixed ball 9 of guiding fixed groove 8.ByThis, in detection kit 2 complete sampling and test paper detection after, will test kit 2 be inserted into detection device 3 in and detection device3 are fixedly connected, and will test probe 101 and are inserted in detection kit 2 across detection hole 7, detect to test paper 5.
As shown in Figure 1,2 housing top surface of detection kit is equipped with the sample holes 10 cooperated with probe tube 4, detection kit 2Housing sidewall is additionally provided with the first fixing clamp 11 for fixing the probe tube 4.As a result, probe tube 4 complete sampling after, by intoSample hole 10 is inserted to detection kit 2, then by the first fixing clamp 11 that probe tube 4 is fixed.
As shown in figs. 1 and 3, probe tube 4 includes the first fixing clamp 401 and the sampler space 402, the first fixing clamp from top to bottom401 be open-topped hemi-closure space structure, and the sampler space 402 is the hemi-closure space structure of bottom opening, and first is fixed401 bottoms are pressed from both sides equipped with screw channel 403, screw channel 403 is twined set on 402 outer wall of the sampler space, 403 top of screw channel and theOne fixing clamp 401 communicates, and 403 bottom of screw channel closing, the inner wall that screw channel 403 is in contact with the sampler space 402 is equipped withSeveral through-holes 404 communicated with the sampler space 402.The first fixing clamp 401 can be with by screw channel 403, through-hole 404 as a result,The sampler space 402 communicates.
In addition, middle part is equipped with liquid storage tank 405, the first fixing clamp in the first fixing clamp 401 in order to be rinsed to sample401 tops are equipped with piercing needle 406, and 406 side of piercing needle is equipped with guide post 407, is equipped with and guide post 407 by sample holes 10The guide through hole 408 of cooperation.Liquid storage tank 405, using being formulated, is dilution in liquid storage tank to dilute according to different biological samplesSample reaches detectable concentration.Such as the 20mM PBS (PH that dilution is 100 μ L in detection fecal hemoglobin liquid storage tank7.4), 0.5%BSA, 0.2%Tween 20,0.02%NaN3, the sample size that probe tube picks is 50mg, corresponding detection examinationPaper bag bonding pad containing fluorescent marker, T line and C line, the fluorescent marker bonding pad anti-hemoglobin containing quantum dot-labeled mouseMonoclonal antibody A, T line contains coating anti-hemoglobin monoclonal antibody B, the C line of mouse and contains coating sheep anti mouse polyclonal antibody(Zhuhai Bo Mei Biotechnology Co., Ltd).In this way, pressing piercing needle 406, is allowed to move downward along 407 direction of guide post,After puncturing liquid storage tank 405, downward liquid flow in liquid storage tank 405, threadingly channel 403, by through-hole 404 to the sampler spaceSample is rinsed in 402.
After rinsing sample, usually also need to filter again, so, screen pipe 5 is further equipped in the present invention.For fixationScreen pipe 5,2 housing sidewall of detection kit are additionally provided with the second fixing clamp 12.It can will be filtered by the second fixing clamp 12 as a result,Pipe 5 is fixed.
As shown in Figure 1, screen pipe 5 is upper and lower opening structure, glass fibre membrane 13 is equipped in the middle part of screen pipe 5.As a result, viaSample liquid in liquid storage tank 405 after liquid wash can be filtered again and be mixed indirectly to reach by glass fibre membrane 13The purpose of even sample liquid.
As shown in Fig. 2, 2 outer wall of detection kit, which is sticked, bar code 14,3 inner wall of detection device is equipped with right with bar code 14The barcode recognizer 15 answered.Barcode recognizer 15 reads the bar code 14 in detection kit 2 as a result, to guide inspectionIt surveys instrument and starts detection project program matched with the detection kit 2.
As shown in figures 4 and 8, being fixedly connected in order to facilitate detection kit 2 and detection device 3, detection kit 2 are pushed upFace and bottom surface outer wall are respectively equipped with the first fixed cynapse 16 and the second fixed cynapse 17, and 3 inner wall of detection device is equipped with respectively with theOne fixed cynapse 16 and the second fixed fixed shrinkage pool 18 and second of cynapse 17 corresponding first fix shrinkage pool 19.Thus, it is possible to logicalIt crosses the fixed shrinkage pool 18 of the first fixed cynapse 17, first of fixation cynapse 16, second and the second fixed shrinkage pool 19 reaches further controlThe purpose of detection kit 2 and 3 relative position of detection device.
As shown in Figures 4 and 5, in order to further fix the relative position of 2 box detection device 3 of detection kit, detection device 3Inner sidewall bottom is equipped with guide fixing plate 20, and 20 side of guide fixing plate is fixedly connected with 3 inner sidewall of detection device, fixed ball 9The other side of guide fixing plate 20 is set to by spring 21.
Because of the not reproducible use of detection kit 2, so upon completion of the assays, need to be drawn off and abandon, so,Detection device 3 is outer, detector main body 1 is equipped with spring control switch 22.After the completion of detecting as a result, pass through spring control switch22, which will test kit 2 and fixed ball 9, separates, to take out detection kit 2.
In the present invention, as shown in fig. 6, when detecting fecal hemoglobin, test paper 6 used is to include fluorescent markerThe Test paper of bonding pad, T line and C line, fluorescent marker bonding pad anti-hemoglobin monoclonal antibody containing quantum dot-labeled mouseA, T line contain coating anti-hemoglobin monoclonal antibody B, the C line of mouse and contain coating sheep anti mouse polyclonal antibody (the rich U.S. biology in ZhuhaiScience and Technology Ltd.), for convenience of the placement of test paper 6, it is equipped with test paper platform 601 below test paper 6, is equipped with test paper platform below test paper 6601 constitute detection card.
Existing equipment has can be used in detector main body 1 of the invention, and (such as Nanjing micrometering Biotechnology Co., Ltd is rawIt producesSequence detector) it is reequiped, reequip the simple and convenient increase present invention i.e. on original bodyAdaptation detection device 3.
Use is sampled and detected using the detection device of biological sample quick detection kit and adaptation of the inventionThe step of it is as follows:
Using probe tube 4 pick sample 50mg insertion detection kit 2 push piercing needle 406, wait it is to be diluted after sampleThis liquid is completed and the combination of Test paper, it is then detected that the detection device 3 of the insertion adaptation of kit 2, detector main body 1 are completedDetection.The detection probe 101 of detector main body 1 can be fixed or mobilizable insertion detection kit 2 in, detection examinationAgent box 2 can be equipped with transparent observation window and observe sample process situation, can also be arranged outside corresponding detection device 3 be protected from light it is dust-proofCover board, the opening in detection kit 2 can use tinfoil sealing to throw off or be directly pierced into when using.
The detection of the detection fecal hemoglobin of embodiment 2
With the kit 2 and mating detection device 1 of the embodiment of the present invention 1 and commercial test kit (commercialization detection blocks,Serial single channel fluorescence immunity analyzer) detection hemoglobin fast quantification is carried out to 60 parts of fecal specimensDetection.
Commercial test kit (commercialization detection card): it carries out taking sample preparation according to detection card specification, be loaded and be read outDetect sample operations.
The data obtained is as follows:
It is sampled detection using the biological sample quick detection kit of embodiment 1 and the detection device of adaptation, as a resultIt is as follows:
| Sample ID | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Kit of the present invention | + | + | + | + | + | + | + | + | + | + |
| Commercial kits | + | + | + | + | + | + | + | + | + | + |
| Sample ID | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| Kit of the present invention | + | + | + | + | + | + | + | + | + | + |
| Commercial kits | + | + | + | + | + | + | + | + | + | + |
| Sample ID | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| Kit of the present invention | + | + | + | + | + | + | + | + | + | + |
| Commercial kits | + | + | + | + | + | + | + | + | + | + |
| Sample ID | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
| Kit of the present invention | + | + | + | + | + | - | - | - | - | - |
| Commercial kits | + | + | + | + | - | - | - | - | - | - |
| Sample ID | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 |
| Kit of the present invention | - | - | - | - | - | - | - | - | - | - |
| Commercial kits | - | - | - | - | - | - | - | - | - | - |
| Sample ID | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
| Kit of the present invention | - | - | - | - | - | - | - | - | - | - |
| Commercial kits | - | - | - | - | - | - | - | - | - | - |
Note: the "+" representative detection positive, "-" represent detection feminine gender in table.
Basic statistical data are carried out to the data obtained to be as follows:
Its positive coincidence rate=34/34*100%=100.00%；Negative match-rate=25/26*100%=96.15%；Positive desired value=34/35*100%=97.14%；Negative desired value=25/25*100%=100.00%；Total coincidence rate=59/60*100%=98.33%；Kappa consistency check: Kappa value be 0.9659, indicate two detection kit consistency compared withIt is good.It to sum up tests, it is believed that two kinds of kits fecal hemoglobins can be replaced mutually the result is that of equal value.
It can be seen that the present invention can be used for detection of biological samples without complicated sample process process, it is more convenient non-The use of professional, such as the use of domestic consumer or non-verification section doctor, while professional is using can also reduce workWork amount can be with the processing sample of rapid, high volume.
Above-described is only some embodiments of the present invention.For those of ordinary skill in the art, notUnder the premise of being detached from the invention design, various modifications and improvements can be made, these belong to protection model of the inventionIt encloses.