Disclosure of Invention
The invention aims to provide a magnetic bead cleaning device, which solves the problems of complicated process and complex flow during magnetic bead cleaning and waste liquid suction in the prior art.
Another object of the present invention is to provide a chemiluminescent immunoassay analyzer capable of achieving the running water washing of magnetic beads.
In order to achieve the above object, the technical scheme adopted by the embodiment of the invention is as follows:
a magnetic bead washing apparatus comprising: the reaction cup tray is provided with a plurality of hole sites for placing reaction cups; the reaction cup disc rotates intermittently according to the period; the cleaning assembly comprises a liquid suction needle set and a cleaning plate set; the cleaning plate set is arranged on the reaction cup disc, the liquid suction needle set can penetrate through the cleaning plate set to extend into the reaction cup to suck waste liquid, and the cleaning plate set is provided with a cleaning tube set; and a magnetic adsorption assembly including a magnetic adsorption part for adsorbing the magnetic beads and a driving part; the magnetic adsorption part is connected with the reaction cup disc; the driving part is connected with the magnetic adsorption part in a transmission way; wherein, the driving part is configured to drive the magnetic adsorption part to move towards the direction close to the reaction cup when the liquid suction needle group sucks the waste liquid in the reaction cup so as to adsorb the magnetic beads on the side wall of the reaction cup.
In a preferred embodiment of the present invention, the magnetic attraction portion includes a magnet attraction plate, a magnet set and a magnet base; the magnet group is connected with the magnet adsorption plate, the magnet adsorption plate is connected with the magnet seat, and the driving part is in transmission connection with the magnet seat; the magnet group is arranged below the cleaning plate group and connected with the reaction cup disc, and when the driving part drives the magnet seat to be close to the reaction cup, the magnetic beads in the reaction cup are adsorbed on the cup wall.
In a preferred embodiment of the present invention, the driving part is a screw motor, and the screw motor is connected to the magnet base in a transmission manner.
In a preferred embodiment of the present invention, the cleaning plate set includes a cleaning plate and a cleaning block; the cleaning block is arranged on the cleaning plate, and the liquid suction needle group passes through the cleaning block; the cleaning tube group comprises a first cleaning tube and a second cleaning tube, the first cleaning tube is arranged on the cleaning plate, the second cleaning tube is arranged on the cleaning block, and the first cleaning tube is configured to clean the reaction cup; the second cleaning tube is configured for cleaning the pipetting needle set.
In a preferred embodiment of the present invention, the cleaning block includes a first cleaning block and a second cleaning block, and the first cleaning block is provided with a second cleaning tube.
In a preferred embodiment of the present invention, the second cleaning tube comprises a liquid suction tube and a liquid discharge tube, and both the liquid suction tube and the liquid discharge tube are communicated with the cleaning block.
In a preferred embodiment of the present invention, the pipetting needle set includes a pipetting needle body, a driving mechanism and a linear guide rail; the driving mechanism is connected with the liquid suction needle body in a transmission way; the linear guide rail is connected with the reaction cup disc; the driving mechanism is configured to drive the liquid sucking needle body to move up and down along the linear guide rail so as to extend into the reaction cup to suck the waste liquid.
In the preferred embodiment of the invention, the liquid suction needle group comprises a first liquid suction needle group and a second liquid suction needle group, and the first liquid suction needle group and the second liquid suction needle group are sequentially arranged at different hole sites of the reaction cup disc; the first liquid suction needle group is matched with the first cleaning block; the second liquid sucking needle set is matched with the second cleaning block.
In the preferred embodiment of the invention, the reaction cup disk comprises a turntable body and a driving device, wherein the driving device is connected with the turntable body in a transmission way so as to drive the turntable body to intermittently rotate according to the period; a groove is formed in the periphery of the turntable body, and a plurality of hole sites are sequentially formed in the groove.
A chemiluminescent immunoassay analyzer comprises the magnetic bead cleaning device.
The beneficial effects of the invention are as follows:
The invention provides a magnetic bead cleaning device which comprises a reaction cup disc, a cleaning assembly and a magnetic adsorption assembly. Wherein, be provided with a plurality of hole sites that are used for placing the reaction cup on the reaction cup dish, the reaction cup dish is rotatory according to the week period intermittent type. The cleaning assembly comprises a liquid suction needle set and a cleaning plate set, the cleaning plate set is arranged on the reaction cup disc, the liquid suction needle set can penetrate through the cleaning plate set to extend into the reaction cup to suck waste liquid, and the cleaning plate set is provided with a cleaning tube set. The magnetic adsorption component comprises a magnetic adsorption part for adsorbing magnetic beads and a driving part, wherein the magnetic adsorption part is connected to the reaction cup disc, and the driving part is in transmission connection with the magnetic adsorption part. Wherein, the driving part is configured to drive the magnetic adsorption part to move towards the direction close to the reaction cup before the liquid suction needle group sucks the waste liquid in the reaction cup so as to adsorb the magnetic beads on the side wall of the reaction cup. This magnetic bead cleaning device, the in-process that washs the subassembly and need absorb the waste liquid, with the magnetic bead absorption, the convenient waste liquid that washs the subassembly and absorb. The magnetic bead cleaning device structurally integrates three functions of magnetic bead adsorption, waste liquid suction and cleaning liquid filling, and realizes the pipelining cleaning of the magnetic beads. The device has the advantages of simple structure, convenience in control, improvement in cleaning quality and guarantee of accuracy of subsequent measurement results.
The invention provides a chemiluminescent immunoassay analyzer which comprises the magnetic bead cleaning device. By arranging the magnetic bead cleaning device, the chemiluminescent immunoassay analyzer can realize the pipelining cleaning of the magnetic beads.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In describing embodiments of the present invention, it should be noted that, the azimuth or positional relationship indicated by the terms "upper", "inner", etc. are based on the azimuth or positional relationship shown in the drawings, or the azimuth or positional relationship that the inventive product is conventionally put in use, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
First embodiment
Referring to fig. 1-4, the present embodiment provides a magnetic bead cleaning apparatus 100, which includes a reaction cup plate 110, a cleaning assembly 120, and a magnetic adsorption assembly 130. The cleaning assembly 120 is disposed in a cleaning area of the cuvette tray 110, and is used for sequentially sucking the waste liquid from each cuvette 150 passing through the cleaning area and adding a cleaning solution to clean the magnetic beads. The magnetic adsorption component 130 is connected to the reaction cup 110, and adsorbs the magnetic beads in the process that the cleaning component 120 needs to absorb the waste liquid, so that the cleaning component 120 can absorb the waste liquid conveniently. The magnetic bead cleaning device 100 has two functions of adsorbing and absorbing waste liquid and adding cleaning liquid to clean the magnetic beads in a structurally integrated manner, and realizes the pipelining cleaning of the magnetic beads.
Further, the reaction cup tray 110 includes a turntable body 111 and a driving device 112, and the driving device 112 is drivingly connected to the turntable body 111 to drive the turntable body 111 to rotate intermittently.
Further, in the present embodiment, the driving device 112 includes a motor and a gear assembly. The motor is connected to the gear assembly, and the gear assembly is connected to the turntable body 111, so that when the motor rotates, the turntable body 111 rotates at intervals of one hole site 113 every other fixed period under the drive of the gear assembly, and the reaction cup 150 continuously enters the cleaning area.
It should be understood that the fixed period of rotation of the turntable body 111 described above is set according to actual needs.
Further, a groove is provided along the circumferential direction of the turntable body 111, and a plurality of hole sites 113 are provided in the groove in sequence. The plurality of holes 113 are used for placing the reaction cup 150.
Further, the reaction cup 150 contains a reaction liquid containing a waste liquid and magnetic beads. A plurality of reaction cups 150 are sequentially placed in each hole site 113, the reaction cup tray 110 rotates intermittently according to a cycle, when each hole site 113 passes through the cleaning assembly 120, the reaction cup tray 110 stops for a certain time, the magnetic adsorption assembly 130 adsorbs magnetic beads in the reaction cups 150, the cleaning assembly 120 sucks waste liquid in the reaction cups 150, and the reaction cups 150 and the sucking needles are cleaned. Then the reaction cup plate 110 continues to rotate, and the reaction cup 150 in the next hole site 113 reaches the cleaning assembly 120 to realize the flow cleaning.
In this embodiment, the number of holes 113 is 45. In other alternative embodiments, the number of holes 113 may be selected according to actual needs.
Further, the cleaning assembly 120 includes a set of pipetting needles 121 and a set of cleaning plates 122. The cleaning plate set 122 is disposed on the reaction cup tray 110, the liquid suction needle set 121 can penetrate through the cleaning plate set 122 and extend into the reaction cup 150 to suck the waste liquid, and the cleaning plate set 122 is provided with a cleaning tube set 123.
Further, a cover plate 114 is provided on the cuvette tray 110. The set of purge plates 122 are attached to the cover plate 114 and are located at the edge of the cuvette tray 110. The area where the cleaning plate set 122 is located is the cleaning area of the reaction cup tray 110.
Further, the cleaning plate set 122 includes a cleaning plate 1221 and a cleaning block. The cleaning block is provided on the cleaning plate 1221, and the pipette needle group 121 passes through the cleaning block.
Specifically, in the present embodiment, the cleaning plate 1221 includes two. Two wash plates 1221 are disposed in sequence in the wash zone. The cleaning blocks include a first cleaning block 1223 and a second cleaning block 1224. On the first cleaning plate 1221, two first cleaning blocks 1223 are provided; two second cleaning blocks 1224 are provided on the second cleaning plate 1221.
Further, the cleaning tube set 123 includes a first cleaning tube 124 and a second cleaning tube 125, the first cleaning tube 124 being disposed on the cleaning plate 1221, the second cleaning tube 125 being disposed on the first cleaning block 1223, the first cleaning tube 124 being configured for cleaning the reaction cup 150; the second cleaning tube 125 is configured to clean the pipetting needle set 121.
Specifically, the first cleaning tube 124 is a steel tube, and the steel tube is connected to the cleaning plate 1221, and in use, cleaning liquid is sprayed into the reaction cup 150 below the cleaning plate 1221 through the steel tube to clean the magnetic beads in the reaction cup 150. In the present embodiment, two first cleaning pipes 124 are provided on the first cleaning plate 1221, and one first cleaning pipe 124 is provided on the second cleaning plate 1221.
In other embodiments, the number of the first cleaning tubes 124 may be selected according to actual needs.
Further, in the present embodiment, the second cleaning pipes 125 are provided on both the first cleaning blocks 1223 on the first cleaning plate 1221. The second cleaning pipes 125 are not provided on both the second cleaning blocks 1224 on the second cleaning plate 1221. The second cleaning tube 125 includes a pipette and a spitting tube, both of which are communicated with the inside of the first cleaning block 1223. The liquid suction pipe and the liquid discharge pipe are bent steel pipes, one liquid suction pipe and one liquid discharge pipe are used for cleaning the liquid suction needle body 143.
In other embodiments, the number of pipettes and spitting tubes is selected according to actual needs.
Further, in the present embodiment, the pipetting needle assembly 140 includes a first pipetting needle assembly 141 and a second pipetting needle assembly 142, and the first pipetting needle assembly 141 and the second pipetting needle assembly 142 are sequentially disposed at different hole sites 113 of the cuvette tray 110. First pipetting needle assembly 141 mates with first rinse block 1223; second pipetting needle set 142 is engaged with second rinse block 1224.
Further, the first pipetting needle group 141 and the second pipetting needle group 142 each include two pipetting needle bodies 143. A pipette needle body 143 is engaged with a cleaning block.
Further, each of the pipette needle bodies 143 is capable of moving up and down with respect to the cuvette tray 110, thereby sucking up the waste liquid in the cuvette 150 placed in the washing zone of the cuvette tray 110.
Further, the liquid sucking needle body 143 is driven by the driving mechanism 144 to move up and down along the linear guide 145.
Specifically, in the present embodiment, the driving mechanism 144 is a screw motor.
Further, to prevent the slider of the linear guide 145 from being separated from the linear guide 145, a stopper mechanism 146 is provided. Further ensuring the reliability of the pipetting needle assembly 140 for pipetting waste liquid.
Further, the magnetic attraction assembly 130 includes a magnetic attraction portion for attracting magnetic beads and a driving portion 131. The magnetic adsorption part is connected to the reaction cup plate 110, and the driving part 131 is connected to the magnetic adsorption part in a transmission manner. The driving part 131 is configured to drive the magnetic adsorbing part to move in a direction approaching the magnetic beads to adsorb the magnetic beads on the magnetic adsorbing part when the liquid sucking needle group 140 sucks the waste liquid in the reaction cup 150.
Specifically, in the present embodiment, the magnetic attraction portion includes a magnet attraction plate 133, a magnet group 134, and a magnet holder 135. Further, the magnet group 134 is connected to the magnet attraction plate 133, the magnet attraction plate 133 is connected to the magnet base 135, and the driving part 131 is drivingly connected to the magnet base 135.
Further, the magnet set 134 is disposed below the cleaning plate set 122 and connected to the reaction cup tray 110, and when the driving portion 131 drives the magnet base 135 to approach the reaction cup tray 110, the magnet set 134 is attracted to the reaction cup 150 to attract the magnetic beads in the reaction cup 150.
Specifically, in the present embodiment, the magnet group 134 includes three pairs of magnet groups 134, and a total of six arc magnets. Six arc magnets are all attracted to the magnet attraction plate 133, and the magnet attraction plate 133 is mounted on the magnet base 135.
Further, in the present embodiment, the driving part 131 is a screw motor. The magnet base 135 can be driven by a screw motor to move back and forth along a linear slide rail arranged on the magnet base 135 in a direction approaching or separating from the reaction cup tray 110. Specifically, before the liquid sucking needle extends into the reaction cup 150 to suck liquid, the magnet seat 135 moves forward to adsorb the magnetic beads on the arm of the reaction cup 150; after the liquid sucking needle finishes liquid sucking and returning, the magnet seat 135 leaves the reaction cup 150, and the cleaning steel pipe sprays cleaning liquid to clean the magnetic beads.
Referring to fig. 1, in the present embodiment, the three pairs of arc magnets form an arc, which can simultaneously perform the adsorption of the magnetic beads in the seven reaction cups 150, and the seven holes 113 are numbered from one to seven in a counter-clockwise direction. Four liquid suction needles are respectively positioned on the two holes 113, the four holes 113, the six holes 113 and the seven holes 113. The cuvette tray 110 is rotated one hole site 113 at a time under the drive of the drive means 112 according to the cycle time T. When the reaction cup 150 to be cleaned exists in the holes 113 from one to seven, the arc magnet moves forwards along the linear slide rail and is close to the outer wall of the reaction cup 150 under the driving of the driving part 131 in each period time T. The magnetic beads in the reaction cup 150 are attracted to the inner wall of the reaction cup 150 by the magnetic field. Near the end of the cycle time T, the arcuate magnet is retracted to the initial position.
The magnetic bead washing apparatus 100 performs the washing process as follows:
Step1: the cuvette tray 110 rotates periodically and the cuvette 150 to be cleaned first enters the first position under the rotation of the cuvette tray 110. At this time, the magnet holder 135 is driven by the driving part 131 to move forward along the linear rail, so that the arc magnet approaches the reaction cup 150. The magnetic beads in the reaction cup 150 are adsorbed on the wall of the reaction cup 150 under the influence of the magnetic field, and after a period of time, the driving part 131 reversely rotates, so that the arc-shaped magnet leaves the reaction cup 150 and returns to the original position.
Step2: the cuvette tray 110 rotates in a periodic fashion to deliver the cuvette 150 to position number two. The driving part 131 repeats the operation of step1, and after the reaction cup 150 stays at the second position for a period of time, the liquid suction needle body 143 above the second position descends into the reaction cup 150 along the linear guide 145 under the action of the driving mechanism 144, sucks the liquid in the reaction cup 150, and then withdraws from the reaction cup 150. In the process of exiting the reaction cup 150, the liquid suction needle body 143 passes through the first cleaning block 1223, the second cleaning tube 125 on the first cleaning block 1223, a liquid discharge, a liquid suction, and the residual waste liquid on the outer wall of the liquid suction needle body 143 is cleaned. After the liquid sucking needle body 143 is restored to the original position, the magnet seat 135 is far away from the reaction cup 150 along the linear slide rail under the action of the driving part 131, and the magnetic beads in the reaction cup 150 are not affected by the magnetic field. At this time, the first cleaning tube 124 sprays cleaning liquid onto the sidewall of the cuvette 150, and breaks up the magnetic beads adsorbed on the wall of the cuvette 150, thereby cleaning the cuvette.
Step3: after the cleaning, the reaction cup tray 110 rotates periodically to send the reaction cup 150 to the third position, and the arc magnet repeats the action in step1
Step4: the cuvette tray 110 continues to rotate in cycles to position number four. The action of each component on the fourth bit is the same as the action of the second bit in step 2.
Step5: the cuvette tray 110 rotates periodically to deliver the cuvette 150 to position five and the arc magnet repeats the step1 motion.
Step6: the cuvette tray 110 rotates periodically to transfer the cuvette 150 to the sixth position (the second cleaning block 1224 provided at the sixth position is not provided with the second cleaning tube 125, so that the pipette body 143 is not cleaned). The liquid sucking needle body 143 on the sixth position descends into the reaction cup 150 along the linear guide 145 under the action of the driving mechanism 144, sucks the liquid in the reaction cup 150, and then withdraws from the reaction cup 150. After the liquid sucking needle body 143 is restored to the original position, the magnet seat 135 is far away from the reaction cup 150 along the linear slide rail under the action of the driving part 131, and the magnetic beads in the reaction cup 150 are not affected by the magnetic field. At this time, the first cleaning tube 124 sprays cleaning liquid onto the sidewall of the cuvette 150, and breaks up the magnetic beads adsorbed on the wall of the cuvette 150, thereby cleaning the cuvette.
Step7: the cuvette tray 110 rotates in a periodic manner to deliver the cuvette 150 to position seven. The arc magnet is close to the wall of the reaction cup 150 under the action of the driving part 131, and the magnetic beads sprayed by the first cleaning tube 124 are gathered again on the wall of the reaction cup 150 under the action of the magnetic field. After the reaction cup 150 stays at the seventh position for a period of time, the liquid suction needle body 143 above the seventh position descends into the reaction cup 150 along the linear guide rail 145 under the action of the driving mechanism 144, sucks the liquid in the reaction cup 150, and then withdraws from the reaction cup 150 to complete the cleaning of the magnetic beads.
Second embodiment
The present embodiment provides a chemiluminescent immunoassay analyzer comprising the magnetic bead washing device provided in the first embodiment. By arranging the magnetic bead cleaning device, the chemiluminescent immunoassay analyzer can realize the pipelining cleaning of the magnetic beads.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.