A kind of environmental detection set and its manufacturing method suitable for poct class diagnostic reagentsTechnical field
The present invention relates to poct diagnostic fields, the detection device of especially a kind of auxiliary poct diagnostic reagents detection environment andIts manufacturing method.
Background technology
Poct refers to a kind of diagnostic check carried out by non-Lab workers.Close at Case treatment, by not receivingThe clinical laboratory inspection that the clinical staff of clinical labororatory's subject training or patient's (self detection) carry out.Poct is to passThe at-once monitor equipment of the inspection carried out other than system, core or central laboratory.The check system of poct will be closer to trouble at presentPerson is simultaneously distributed in hospital, community clinic and familial self monitoring field etc..
Poct class diagnostic reagents have easy to operate, field monitoring, sample disposal require it is low, quickly provide result, examineAs a result the advantages that being comparable.Small-sized testing instruments are usually applicable in, are clinical examination center high pass amount, large automatic inspectionStrong supplement, have the characteristics that certain.But many poct class diagnostic reagents are very big by environmental influence.It is commented using room interstitialWhether testing result of the sample under different humitures reaches in the standard of biochemical instruments test value (target value), and conclusion is test environmentHumiture is affected to poct class diagnostic reagent testing results.
Poct class diagnostic reagents have it is small, it is easy to carry, test out the result time is short, sample disposal require it is low excellentPoint is widely applied to the fields such as hospital, community clinic and household self-monitoring at present.Poct class diagnostic reagents divide when detectingFor two classes, for one kind there is provided a kind of detector, which provides testing result.One kind is the diagnosis examination of poct classesAgent does not have the participation of detector, individually provides testing result.Poct classes diagnostic reagent when detecting, can directly display test knotFruit, but poct classes diagnostic reagent can have the following disadvantages when detecting：It cannot ensure whether the test environment of user meets poctThe requirement of class diagnostic reagent technology, if the test environment of user does not operate under desired test environment, will eventually influenceThe accuracy of testing result.
Market needs a kind of poct diagnostic reagents of included temperature-humidity detecting device, can allow user's voluntarily discriminating test ringWhether the humiture in border meets the requirement of poct class diagnostic reagent technologies, and the present invention solves this problem.
Invention content
To solve the deficiencies in the prior art, the purpose of the present invention is to provide a kind of rings suitable for poct class diagnostic reagentsTemperature and Humidity item is arranged, convenient for users to voluntarily in border detection device and its manufacturing method on poct class diagnostic reagent carriersWhether the humiture of judgement poct classes diagnostic reagent environment when in use meets product technology requirement；Humiture identification point structureSimply, judgment method is intuitive, is easy observation, also brings facility to the use of operator.
In order to realize that above-mentioned target, the present invention adopt the following technical scheme that：
A kind of environmental detection set suitable for poct class diagnostic reagents, including：It is fixed on poct class diagnostic reagent carriersOn temperature develop the color item.
A kind of environmental detection set suitable for poct class diagnostic reagents above-mentioned, temperature develop the color item by temperature chromogenic reactionSlurry is formed；Temperature chromogenic reaction slurry has according to mass percent composition：The solvent of 57%-63%, the tree of 27%-33%Fat, the surfactant of 0.3%-0.9%, the temperature-sensitive toner of 7%-13%；Temperature-sensitive toner is passed through by Reversible Thermochromic CompoundsElectronics transfer is prepared.
A kind of environmental detection set suitable for poct class diagnostic reagents, which is characterized in that including：Poct classes are fixed on to examineHumidity chromogenic item on disconnected reagent carrier.
A kind of environmental detection set suitable for poct class diagnostic reagents above-mentioned, humidity chromogenic item are reacted by humidity chromogenicSlurry is formed；Humidity chromogenic reaction paste has according to mass percent composition：The solvent of 57%-63%, the tree of 27%-33%Fat, the surfactant of 0.3%-0.9%, the wet toner of sense of 7%-13%；Feel wet toner to be placed in silicon substrate carrier by colorantIt is prepared.
A kind of environmental detection set suitable for poct class diagnostic reagents, including：It is fixed on poct class diagnostic reagent carriersOn temperature colour developing item and humidity chromogenic item.
A kind of environmental detection set suitable for poct class diagnostic reagents above-mentioned, temperature develop the color item by temperature chromogenic reactionSlurry is formed；The temperature chromogenic reaction slurry has according to mass percent composition：The solvent of 57-63%, the tree of 27-33%Fat, the surfactant of 0.3%-0.9%, the temperature-sensitive toner of 7%-13%；Temperature-sensitive toner is passed through by Reversible Thermochromic CompoundsElectronics transfer is prepared.
Humidity chromogenic item is formed by humidity chromogenic reaction paste；The humidity chromogenic reaction paste is according to mass percent groupCheng You：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%, the wet toner of sense of 7%-13%；Feel wet toner and be placed in silicon substrate carrier by colorant and is prepared.
A kind of manufacturing method of environmental detection set suitable for poct class diagnostic reagents, including：
Configure temperature chromogenic reaction slurry；Temperature colour developing item is formed by temperature chromogenic reaction slurry；Temperature chromogenic reaction is starchedMaterial has according to mass percent composition：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%,The temperature-sensitive toner of 7%-13%；Temperature-sensitive toner is prepared by Reversible Thermochromic Compounds by electronics transfer；By solvent, treeFat, surfactant, temperature-sensitive toner are in uniform flow-like according to proportioning successively uniform dissolution；
Configure humidity chromogenic reaction paste；Humidity chromogenic item is formed by humidity chromogenic reaction paste；Humidity chromogenic reaction slurryMaterial has according to mass percent composition：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%,The wet toner of sense of 7%-13%；Feel wet toner and be placed in silicon substrate carrier by colorant and is prepared；By solvent, resin, surface is livedProperty agent, feel wet toner according to proportioning uniform dissolution successively, be in uniform flow-like；
The temperature chromogenic reaction slurry, humidity chromogenic reaction paste are printed on poct classes and examined by screen printing techniqueOn the carrier of disconnected reagent；
Carrier after printing is placed in baker and cured, dried.
A kind of manufacturing method of environmental detection set suitable for poct class diagnostic reagents above-mentioned,
Screen printing technique is using film thickness 20um, 300 mesh of mesh number, 22.5 ° of angle, the silk printing screen of 21 ± 1N of tensionVersion；
A kind of manufacturing method of environmental detection set suitable for poct class diagnostic reagents above-mentioned, before silk-screen printing,It needs to adjust screen printer print parameter；
Specific silk-screen parameter is as follows：Screen printer air pressure should be within the scope of 6.0 ± 1.0Bar；It is required that silk-screen figure is in scraperCenter and, the angle of scraper and halftone parallel with scraper：75°；The speed of service：240mm/s±100mm/s；The fortune of ink-retruning bladeScanning frequency degree：240mm/s±100mm/s；Table top halftone spacing：4.0mm±1.0mm.
A kind of manufacturing method of environmental detection set suitable for poct class diagnostic reagents above-mentioned, baker are dried using IRMain line, drying, solidification design parameter are as follows：
Spin manifold temperature is set as 110 DEG C, and the temperature in oven body is made to control at 110 DEG C ± 2 DEG C；
Transmission net tape speed：Ensure that by the time for keeping the temperature effective coverage be 6 ± 1min；Corresponding guipure speed is 0.8M/min.
The invention has the beneficial effects that：
Temperature and Humidity item is set on poct class diagnostic reagent carriers, convenient for users to voluntarily judging the diagnosis examination of poct classesWhether the humiture of agent environment when in use meets product technology requirement；
Temperature and Humidity item is fixed on carrier by the present invention by screen printing technique, and plate-making is easy with printing process,Equipment investment is few, at low cost.It is not limited by printing species, size, shape.The ink and coating of different solvents can be selectedAdapt to the printing of different type stock, and due to silk-screen plate softness, required printing pressure is small, can carry out curved surface, breakable objectPrinting.Printer's ink thickness, bright in luster, three-dimensional sense and strong covering power.4. not limited by ink and diluent, can print resistance toThe strong printed matter of photosensitiveness.
Warm color-change powder is a kind of microcapsules changing color with temperature rise or decline, is organised by electron transfer typeClose prepared by objects system.Electron transfer type organic compound is a kind of organic chromophoric system with special chemical structure.In spyDetermine because electronics transfer makes the molecular structure of the organic matter change at temperature, to realize color change.The test model of temperatureIt is 0 DEG C~45 DEG C to enclose, and temperature is revealed as colorless state at test environment≤32 DEG C, when 32 DEG C of >, is revealed as pink colour state.The test scope 30%RH-90%RH of humidity, the i.e. chromogenic reaction of humidity can with the increase of humidity, color from depth to shallow, mostAfter become colorless.Humiture identifies that judgment method is intuitive, is easy observation, also brings facility to the use of operator.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of embodiment of poct diagnostic reagents carrier of the present invention.
Specific implementation mode
Specific introduce is made to the present invention below in conjunction with the drawings and specific embodiments.
A kind of environmental detection set suitable for poct class diagnostic reagents, including：It is fixed on poct class diagnostic reagent carriersOn temperature colour developing item and humidity chromogenic item.It should be noted that can there was only temperature colour developing item or temperature colour developing on carrierItem one of them, and develop the color item number be not limited.
Temperature colour developing item is formed by temperature chromogenic reaction slurry；Temperature chromogenic reaction slurry is formed according to mass percentHave：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%, the temperature-sensitive toner of 7%-13%；SenseWarm colour powder is prepared by Reversible Thermochromic Compounds by electronics transfer.
Humidity chromogenic item is formed by humidity chromogenic reaction paste；Humidity chromogenic reaction paste is formed according to mass percentHave：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%, the wet toner of sense of 7%-13%.SenseWet toner, which is placed in by colorant in silicon substrate carrier, to be prepared.
A kind of manufacturing method of environmental detection set suitable for poct class diagnostic reagents, including：
Configure temperature chromogenic reaction slurry；Temperature colour developing item is formed by temperature chromogenic reaction slurry；Temperature chromogenic reaction is starchedMaterial has according to mass percent composition：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%,The temperature-sensitive toner of 7%-13%；Temperature-sensitive toner is prepared by Reversible Thermochromic Compounds by electronics transfer；By solvent, treeFat, surfactant, temperature-sensitive toner are in uniform flow-like according to proportioning successively uniform dissolution.
As a kind of embodiment, solvent can select methanol, DMF, ethylene glycol diethyl ether, toluene, cyclohexanone, benzyl alcohol,One or more combinations in dimethylbenzene, hexamethylene.
As a kind of embodiment, resin uses the resin of high solids low-viscosity (low polymerization degree or low molecular weight), can be withSelect epoxy resin, phenolic resin, Lauxite, melamine resin, furane resins, organic siliconresin, polyester resin,One kind in polyamide, acrylic resin, polyurethane, vinylite, hydrocarbon resins, polyethers resinoid, vinyl chloride-vinyl acetate resinOr multiple combinations.
As a kind of embodiment, surfactant is polyether-modified organosilicon, ethylene oxide adduct and fluoropolymerThe fatty acid ester compounded object of object, silicone emulsion, higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy propyl alcoholOne or more combinations in amidogen ether, polypropylene glycerol aether and polyoxypropylene.
As a kind of embodiment, temperature-sensitive toner is prepared by Reversible Thermochromic Compounds by electronics transfer；It can backheatingColor compound includes：Loop coil class Reversible Thermochromic Compounds, the Reversible Thermochromic Compounds of more aromatic rings containing CH=CH,One or more combinations of Schiff Reversible Thermochromic Compounds.
Configure humidity chromogenic reaction paste；Humidity chromogenic item is formed by humidity chromogenic reaction paste；Humidity chromogenic reaction slurryMaterial has according to mass percent composition：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%,The wet toner of sense of 7%-13%；Feel wet toner and be placed in silicon substrate carrier by colorant and is prepared；By solvent, resin, surface is livedProperty agent, feel wet toner according to proportioning uniform dissolution successively, be in uniform flow-like.
As a kind of embodiment, solvent can select methanol, DMF, ethylene glycol diethyl ether, toluene, cyclohexanone, benzyl alcohol,One or more combinations in dimethylbenzene, hexamethylene.
As a kind of embodiment, resin uses the resin of high solids low-viscosity (low polymerization degree or low molecular weight), can be withSelect epoxy resin, phenolic resin, Lauxite, melamine resin, furane resins, organic siliconresin, polyester resin,One kind in polyamide, acrylic resin, polyurethane, vinylite, hydrocarbon resins, polyethers resinoid, vinyl chloride-vinyl acetate resinOr multiple combinations.
As a kind of embodiment, surfactant is polyether-modified organosilicon, ethylene oxide adduct and fluoropolymerThe fatty acid ester compounded object of object, silicone emulsion, higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy propyl alcoholOne or more combinations in amidogen ether, polypropylene glycerol aether and polyoxypropylene.
Feel wet toner and be placed in silicon substrate carrier by colorant and is prepared；As a kind of embodiment, colorant can be iron(II) and/or activated indicators as iron (III) salt or salt can also be, acid-base indicator；As a kind of embodiment, acidNeutralization indicator includes：One or more combinations in reindeer moss, crystal violet, Bromothymol blue, xylenol basket.Colorant withWater combines, and color changes.
Temperature chromogenic reaction slurry, humidity chromogenic reaction paste are printed on the diagnosis examination of poct classes by screen printing techniqueOn the carrier of agent；Screen printing technique is using film thickness 20um, 300 mesh of mesh number, 22.5 ° of angle, the silk-screen of 21 ± 1N of tensionHalftone.
Before silk-screen printing, need to adjust screen printer print parameter；Specific silk-screen parameter is as follows：Screen printer air pressure should beWithin the scope of 6.0 ± 1.0Bar；It is required that silk-screen figure is in scraper center and, the angle of scraper and halftone parallel with scraper：75°；The speed of service：240mm/s±100mm/s；The gap of ink-retruning blade and halftone screen cloth is about the thickness of an A4 paper, to be protectedHold appropriate carbon slurry thickness uniform fold whole silk-screen graphics field；The speed of service：240mm/s±100mm/s；Between table top halftoneAway from：4.0mm±1.0mm；And examination printing is carried out after being aligned to silk-screen halftone.Under process above printing parameter, test stripsPerformance it is best, desired use of otherwise arriving.
Carrier after printing is placed in baker and cured, dried.As a kind of embodiment, baker uses IRDrying line, drying, solidification design parameter are as follows：Spin manifold temperature is set as 110 DEG C, and the temperature in oven body is made to control at 110 DEG C±2℃。
Transmission net tape speed：Ensure that by the time for keeping the temperature effective coverage be 6 ± 1min；Corresponding guipure speed is 0.8M/min.Under process above printing parameter, the performance of test strips is best, desired use of otherwise arriving.
Upper cover plate after drying sticks in the top of test paper.
The principle of colour developing；Warm color-change powder is a kind of microcapsules changing color with temperature rise or decline, is by electronicsPrepared by transfevent organic compound objects system.Electron transfer type organic compound is a kind of organic hair with special chemical structureColor system.At a certain temperature because electronics transfer makes the molecular structure of the organic matter change, to realize color change.SenseColorant in wet toner can be combined with water, and color changes.Humiture identifies that judgment method is intuitive, is easy observation, also givesThe use of operator brings facility.
User by poct class diagnostic reagents expose under the test environment, the carrier part of poct class diagnostic reagents it is correspondingPrinting part can participate in temperature chromogenic reaction, humidity chromogenic reaction, judge whether the environment meets test request by color.The test scope of temperature is 0 DEG C~45 DEG C, and for temperature at test environment≤32 DEG C, the coloured moiety of silk-screen printing is colourless, >At 32 DEG C, the part of silk-screen printing can show pink colour.The test scope 30%RH-90%RH of humidity, the i.e. chromogenic reaction of humidityIt from depth to shallow, can be eventually become colourless with the increase of humidity, color.
If user uses poct class diagnostic reagents, the temperature identification point pink colour on poct class diagnostic reagent carrier parts,Or the color of the humidity identification point on poct class diagnostic reagent carrier parts is light green color；Then the environment does not meet test and wantsIt asks.
In order to verify the present invention temperature chromogenic reaction slurry, humidity chromogenic reaction paste can be real in ratio rangeIt now detects, does following experiment：
Experiment one：
Temperature chromogenic reaction slurry：Solvent uses：DMF, resin use epoxy resin：Surfactant uses polyoxyethylenePolyoxy propyl alcohol amidogen ether：Temperature-sensitive toner is generated by loop coil class Reversible Thermochromic Compounds in electronics transfer chemical reaction system.
Configure temperature chromogenic reaction slurry；Temperature colour developing item is formed by temperature chromogenic reaction slurry；Temperature chromogenic reaction is starchedMaterial has according to mass percent composition：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%,The temperature-sensitive toner of 7%-13%；
Embodiment 1,57g solvents, 33g resins, 0.3g surfactants, 9.7g temperature-sensitive toners；
Embodiment 2,63g solvents, 27g resins, 0.9g surfactants, 9.1g temperature-sensitive toners；
Embodiment 2,58g solvents, 28.3g resins, 0.7g surfactants, 13g temperature-sensitive toners；
It by same set of technique, is printed on carrier, is individually positioned in 26 DEG C, 32 DEG C, in 36 DEG C of vial, colour developing is such asUnder：
By being tested above it is found that the ratio range according to formula configures, obtained colour developing item all has detectability.
Experiment two：
Humidity chromogenic reaction paste：Solvent uses：DMF, resin use epoxy resin：Surfactant uses polyoxyethylenePolyoxy propyl alcohol amidogen ether：Feel wet toner to be made up of iron (II) salt dipping silicon substrate carrier.
Configure humidity chromogenic reaction paste；Humidity chromogenic item is formed by humidity chromogenic reaction paste；Humidity chromogenic reaction slurryMaterial has according to mass percent composition：The solvent of 57-63%, the resin of 27-33%, the surfactant of 0.3%-0.9%,The wet toner of sense of 7%-13%；
Embodiment 1,57g solvents, 33g resins, 0.3g surfactants, 9.7g feel wet toner；
Embodiment 2,63g solvents, 27g resins, 0.9g surfactants, 9.1g feel wet toner；
Embodiment 2,58g solvents, 28.3g resins, 0.7g surfactants, 13g feel wet toner；
It by same set of technique, is printed on carrier, it is 30%RH, the glass of 60%RH, 90%RH to be individually positioned in humidityIn glass bottle, colour developing is as follows：
By being tested above it is found that the ratio range according to formula configures, obtained colour developing item all has detectability.
The present invention provides a kind of environmental detection set and its manufacturing method suitable for poct class diagnostic reagents, in poct classesTemperature and Humidity item is set on diagnostic reagent carrier, convenient for users to voluntarily judging poct classes diagnostic reagents environment when in useWhether humiture meets product technology requirement；Humiture identification point is simple in structure, and judgment method is intuitive, is easy observation, also givesThe use of operator brings facility.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry shouldUnderstand, the invention is not limited in any way above-described embodiment, all to be obtained by the way of equivalent substitution or equivalent transformationTechnical solution is all fallen in protection scope of the present invention.