Disclosure of Invention
The invention provides an integrated anal detection enrichment sampling tube, a method and application, and aims to solve the problem that in the prior art, when sampling is carried out through an inoculating loop, a sampling rod needs to be pulled out, and liquid on the sampling rod is easy to drip because the pulled sampling rod is not easy to place, so that the environmental pollution is caused.
The technical problem solved by the invention is realized by adopting the following technical scheme: the utility model provides an integral type anus is examined and is increased fungus sampling tube, includes the sampling rod and the transfering loop of integral type structure, the sampling rod link up with the transfering loop mutually.
Further, the sampling rod and the inoculating loop of the integrated structure comprise an inoculating loop arranged in a hollow inner cavity in the sampling rod.
Further, the transfering loop is arranged in the hollow cavity of the sampling rod through the sealing ring.
Furthermore, the sampling rod and the inoculating loop are communicated with each other, and the sampling rod comprises a sampling hole on the surface of the sampling rod and the inoculating loop are communicated with each other.
Further, the sampling rod is inserted into a culture tube, and culture solution is placed in the culture tube.
Furthermore, the sampling rod, the inoculating loop and the culture tube are of an integrated structure.
Further, the transfering loop includes the handle, the handle is connected in the one end of barred body, the other end of barred body is connected in the apical ring.
Meanwhile, the invention also provides an integrated anal detection enrichment sampling method, which comprises the following steps:
the sampling rod of the integrated structure containing the inoculating loop of the integrated anal detection enrichment sampling tube is put into the culture solution in the culture tube for culture after sampling;
if the culture is over 24 hours, pulling out the inoculating loop on the sampling rod, and uniformly smearing the culture on the culture dish through the inoculating loop;
and if the inoculating loop is completely smeared, inserting the inoculating loop back to the sampling rod, and sterilizing at high temperature.
Further, if the inoculating loop is used and the sampling rod is inserted, the sampling rod, the inoculating loop and the culture tube in the integrated structure are disinfected together.
Meanwhile, the invention also provides an integrated anal detection enrichment sampling application, which comprises the integrated anal detection enrichment sampling tube and the integrated anal detection enrichment sampling method preferably used for anal detection enrichment sampling.
The beneficial technical effects are as follows:
1. this patent adopts the sampling rod and the transfering loop of integral type structure, sampling rod and transfering loop link up mutually, because transfering loop, culture tube, sampling rod integral type design, when the inoculation sample, extract the transfering loop, the sampling rod keeps in the test tube, is difficult to the drippage liquid, consequently, effectively prevents the polluted environment.
2. This patent adopts the sampling stick and the transfering loop of integral type structure to include built-in transfering loop of cavity inner chamber in the sampling stick, owing to place in the transfering loop in the sampling stick, it is convenient to gather.
3. This patent adopts place the cavity inner chamber in the sampling rod in transfering loop warp seal ring, because the seal ring has sealed effect, guaranteed that the culture solution in the culture tube does not receive the pollution.
4. The sampling rod with the integrated structure and containing the inoculating loop of the integrated anal detection enrichment sampling tube is adopted, and the sampling rod is placed into a culture solution in a culture tube for culture after sampling; if the culture is over 24 hours, pulling out the inoculating loop on the sampling rod, and uniformly smearing the culture on the culture dish through the inoculating loop; if the inoculating loop is smeared, the inoculating loop is inserted back to the sampling rod and is disinfected and sterilized at high temperature together, and the traditional using mode comprises sampling the sampling rod and then putting the sampling rod into a disposable enrichment culture tube for culture, and after the culture, the sampling rod is taken out, and then the disposable inoculating loop is put into the disposable enrichment culture tube for sampling. And then the sampling rod is inserted back into the disposable enrichment culture tube, after sampling, the plate on the culture dish is scribed for culture, finally the bacteria in the culture dish is observed, and the type of the bacteria is judged, so that the problems that the pulled sampling rod is not easy to place and the liquid on the sampling rod is easy to drip are easily caused.
5. This patent adopts sampling rod and transfering loop and culture tube formula structure as an organic whole and if the transfering loop paints and finishes, then inserts the transfering loop back sampling rod, and high temperature disinfection and sterilization in the lump is because the transfering loop finishes using the back and inserts the test tube, convenient disinfection together.
6. This patent adopts the integral type design, and compact structure is convenient for store and transport.
7. This patent is preferred to be applicable to the anus and examines the enrichment sample.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
in the figure: 1-sampling rod, 2-inoculating loop, 3-sealing loop, 4-sampling hole, 5-culture tube, 6-culture solution, 7-handle, 8-rod body and 9-top loop;
s101, passing the sampling rod of the integrated structure containing the inoculating loop of the integrated anal detection enrichment sampling tube;
s102, sampling and then putting the sample into a culture solution in a culture tube for culture;
s103-if the culture is over 24 hours;
s104, pulling out an inoculating loop on the sampling rod;
s105, uniformly smearing the culture on a culture dish through an inoculating loop;
s106, if the inoculating loop is completely coated;
s107, inserting the inoculating loop back to the sampling rod;
s108-sterilizing at high temperature.
Example (b):
in this embodiment: as shown in figures 1, 3 and 4, the integrated anal detection enrichment sampling tube comprises a sampling rod 1 and aninoculating loop 2 which are of an integrated structure, wherein the sampling rod 1 is communicated with the inoculatingloop 2.
Owing to adopt the sampling rod and the transfering loop of integral type structure, sampling rod and transfering loop link up mutually, because transfering loop, culture tube, sampling rod integral type design, when the inoculation sample, extract the transfering loop, the sampling rod keeps in the test tube, is difficult to the drippage liquid, consequently, effectively prevents the polluted environment.
The sampling rod 1 and the inoculatingloop 2 of the integrated structure comprise the inoculatingloop 2 arranged in a hollow inner cavity in the sampling rod 1.
Because the sampling stick and the transfering loop that adopt integral type structure include built-in transfering loop of cavity inner chamber in the sampling stick, owing to place in the transfering loop in the sampling stick, it is convenient to gather.
The inoculatingloop 2 is arranged in a hollow inner cavity of the sampling rod 1 through a sealingring 3.
Because the inoculating ring is arranged in the hollow inner cavity of the sampling rod through the sealing ring, the sealing ring has the sealing effect, and the culture solution in the culture tube is ensured not to be polluted.
The sampling rod 1 is communicated with the inoculatingloop 2, and the sampling rod 1 is communicated with the inoculatingloop 2 through asampling hole 4 on the surface of the sampling rod 1.
The sampling rod 1 is inserted into aculture tube 5, and aculture solution 6 is arranged in theculture tube 5.
The sampling rod 1, the inoculatingloop 2 and theculture tube 5 are of an integrated structure.
The inoculatingloop 2 comprises ahandle 7, thehandle 7 is connected to one end of arod body 8, and the other end of therod body 8 is connected to atop loop 9.
As shown in fig. 2, meanwhile, the invention also provides an integrated anal detection enrichment sampling method, which comprises the following steps:
after sampling, putting the sampling rod 1 of the integrated structure containing the inoculatingloop 2 of the integrated anal detection enrichment sampling tube into theculture solution 6 in theculture tube 5 for culturing S102;
if the culture is over 24 hours S103, pulling out the inoculating loop 2S104 on the sampling rod 1, and uniformly smearing the culture on a culture dish S105 through the inoculatingloop 2;
if the inoculatingloop 2 is completely smeared S106, the inoculatingloop 2 is inserted back to the sampling rod 1S107, and then sterilized at high temperature S108.
Because the sampling rod with the integrated structure containing the inoculating loop of the integrated anal detection enrichment sampling tube is adopted, the sampling rod is placed into the culture solution in the culture tube for culture after sampling; if the culture is over 24 hours, pulling out the inoculating loop on the sampling rod, and uniformly smearing the culture on the culture dish through the inoculating loop; if the inoculating loop is smeared, the inoculating loop is inserted back to the sampling rod and is disinfected and sterilized at high temperature together, and the traditional using mode comprises sampling the sampling rod and then putting the sampling rod into a disposable enrichment culture tube for culture, and after the culture, the sampling rod is taken out, and then the disposable inoculating loop is put into the disposable enrichment culture tube for sampling. And then the sampling rod is inserted back into the disposable enrichment culture tube, after sampling, the plate on the culture dish is scribed for culture, finally the bacteria in the culture dish is observed, and the type of the bacteria is judged, so that the problems that the pulled sampling rod is not easy to place and the liquid on the sampling rod is easy to drip are easily caused.
If the inoculatingloop 2 is used and the sampling rod 1 is inserted, the sampling rod 1, the inoculatingloop 2 and theculture tube 5 of the integrated structure are disinfected together.
Owing to adopt sampling rod and transfering loop and culture tube formula structure as an organic whole and if transfering loop paints and finishes, then inserts the transfering loop back sampling rod, high temperature disinfection and sterilization in the lump, because the transfering loop finishes using the back and inserts the test tube, convenient disinfection together.
Meanwhile, the invention also provides an integrated anal detection enrichment sampling application, which comprises the integrated anal detection enrichment sampling tube and the integrated anal detection enrichment sampling method preferably used for anal detection enrichment sampling.
Due to the adoption of the integrated design, the structure is compact, and the storage and the transportation are convenient.
The invention is preferably suitable for sampling the anal detection enrichment bacteria.
The working principle is as follows:
according to the integrated anal detection enrichment sampling tube, the sampling rod and the inoculating loop are communicated, due to the integrated design of the inoculating loop, the culture tube and the sampling rod, the inoculating loop is pulled out when inoculation sampling is carried out, the sampling rod is kept in a test tube, and liquid is not easy to drip, and due to the fact that the sampling rod with the integrated structure of the inoculating loop of the integrated anal detection enrichment sampling tube is used for placing the sampling rod into culture solution in the culture tube for culture after sampling; if the culture is over 24 hours, pulling out the inoculating loop on the sampling rod, and uniformly smearing the culture on the culture dish through the inoculating loop; if the inoculating loop is smeared, the inoculating loop is inserted back to the sampling rod and is disinfected and sterilized at high temperature together, and the traditional using mode comprises sampling the sampling rod and then putting the sampling rod into a disposable enrichment culture tube for culture, and after the culture, the sampling rod is taken out, and then the disposable inoculating loop is put into the disposable enrichment culture tube for sampling. The invention solves the problems that the sampling rod needs to be pulled out when sampling is carried out through an inoculating loop in the prior art, and the environment pollution is caused because the pulled out sampling rod is not well placed and the liquid on the sampling rod is easy to drip, and has the beneficial technical effects of effectively preventing the environment pollution, being convenient to collect, being convenient to disinfect together and having compact structure.
The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention to achieve the above technical effects.