A kind of Test paper of water quality total alkalinity and preparation method thereof and detection methodTechnical field
The present invention relates to environmental monitoring and examination of water technical field, more particularly to a kind of Test paper of water quality total alkalinityAnd preparation method thereof and detection method.
Background technology
The abbreviation of water quality (water quality) Water quality.It indicates physics (such as coloration, turbidity, the stink of water bodyDeng), the characteristic of chemical (content of inorganic matter and organic matter) and biology (bacterium, microorganism, planktonic organism, benthon) and itsThe situation of composition.Water quality is to evaluate the situation of Water quality, it is specified that a series of water quality parameters and water standard.As life is drunkThe water standards such as water, industrial water and fishery water.
Water quality total alkalinity refers to the summation of carbanion and bicarbonate ion in water, consumes the total amount of strong acid.CauseThe pollution sources of basicity are mainly the waste water and detergent of the industries such as papermaking, printing and dyeing, chemical industry, plating discharge, chemical fertilizer and pesticide makesWith loss in the process.Basicity is a kind of comprehensive feature index of water, is the important indicator for judging water quality and water processing control.Basicity is also commonly used for buffer capacity and metal dissolubility wherein and toxicity of evaluation water body etc..
Currently, water quality total alkalinity generally use acid-base titration (referring to《Water and effluent monitoring analysis method》),Its principle is：
Water sample is titrated to defined pH value with standard solution, terminal can by the acid-base indicator that is added in the pH value faceThe variation of color judges.When being titrated to phenolphthalein indicator and being become colorless by red, solution ph is 8.3, indicates hydrogen in waterOxygen radical ion has been neutralized, and carbonate is converted into heavy carbonate, reaction equation：
OH-+H+→H2O
CO32-+H+→HCO3-
When being titrated to methyl orange indicator and becoming salmon pink from orange colour, the pH value of solution is 4.4~4.5, instructionHeavy carbonate has been neutralized in water, and reaction is as follows：
HCO3-+H+→H2O+CO2↑
The amount of the Hydrochloric Standard Titration consumed when being reached according to above-mentioned two terminal can calculate carbonic acid in water outletSalt, heavy carbonate and total alkalinity.But the method needs to use the test articles such as acid buret and conical flask, needs water sample being sent toLaboratory is detected, and titration is also required to certain process.Water quality total alkalinity detector also can be used to examine water quality total alkalinityIt surveys.It can be seen that the method detection time of existing detection total alkalinity is long, and need specific test article and instrument and equipment.Therefore,Need a kind of quick, instant, simple measuring means of exploitation.
Invention content
In view of this, the present invention provides a kind of Test paper of water quality total alkalinity and preparation method thereof and detection methods.The Test paper can accurate, simple, quick detection water quality total alkalinity.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of Test papers of water quality total alkalinity, are made of substrate and colour developing indicator paper block, and develop the color test paperBlock is pasted onto substrate one end, and colour developing indicator paper block surface is attached with color developing agent and surfactant；Color developing agent is dimethyl diaminophenazine chloride and phenolphthaleinMixture.
In the present invention, the testing principle of Test paper is：It is aobvious to the variation of ph values in sample using dimethyl diaminophenazine chloride and phenolphthaleinThe property for showing different colours, to determine the value range of total alkalinity.
Preferably, surfactant be polyvinylpyrrolidone, stearic acid, neopelex, quaternary ammonium compound,One or more of lecithin, fatty glyceride, fatty acid sorbitan, polysorbate.
Preferably, substrate is polythene strip or hard paper；
Preferably, the length of substrate is 6~10cm, width is 0.3~1cm.
In embodiment provided by the invention, substrate is polythene strip.
In embodiment provided by the invention, the length of substrate is 10cm, and width is 1cm.
Preferably, colour developing indicator paper block is chromatographic test paper block；
Preferably, the area of colour developing indicator paper block is 0.09~1cm2。
In embodiment provided by the invention, the area for the indicator paper block that develops the color is 1cm2。
Preferably, polyvinylpyrrolidone is PVP K30.
The present invention also provides the preparation methods of the Test paper, include the following steps：
Color developing agent, surfactant are dissolved in ethanol water, obtain maceration extract；Color developing agent is the mixed of dimethyl diaminophenazine chloride and phenolphthaleinClose object；
Colour developing indicator paper block is immersed into maceration extract, is taken out after complete wetting, it is dry, obtain colour developing indicator paper block；
Colour developing indicator paper block is pasted onto substrate one end.
Preferably, dry is forced air drying.
Preferably, the temperature of forced air drying is 30~100 DEG C.
Preferably, the temperature of forced air drying is 80 DEG C.
Preferably, surfactant be polyvinylpyrrolidone, stearic acid, neopelex, quaternary ammonium compound,One or more of lecithin, fatty glyceride, fatty acid sorbitan, polysorbate.
Preferably, in maceration extract, a concentration of 0.1~1.0g/L of dimethyl diaminophenazine chloride, a concentration of 0.1~2.0g/ of phenolphthaleinThe volumn concentration of L, a concentration of 0.1~10g/L of surfactant, ethanol water are 30%~70%.
Preferably, in maceration extract, a concentration of 0.3g/L of dimethyl diaminophenazine chloride, a concentration of 0.5g/L of phenolphthalein, surfactantA concentration of 5g/L, the volumn concentration of ethanol water is 50%.
The present invention also provides a kind of detection methods of water quality total alkalinity, and water is detected using Test paper prepared by the present inventionMatter total alkalinity.
Preferably, the detection method includes the following steps：
Sodium hydroxide solution is prepared to obtain the standard solution of sodium hydroxide of a concentration of 1~500mg/L；
Test paper is immersed in standard solution of sodium hydroxide and is developed the color, color chart is obtained；
Test paper is immersed in sample to be tested and is developed the color, is compared with color chart, obtains the water quality total alkalinity of sample to be tested.
Preferably, a concentration of 10~400mg/L of standard solution of sodium hydroxide.
Preferably, the time immersed in standard solution or sample to be tested is 1~2s.
Preferably, the time immersed in standard solution or sample to be tested is 1s.
Preferably, the time of colour developing is 60~70s.
Preferably, the time of colour developing is 60s.
The present invention provides a kind of Test paper of water quality total alkalinity and preparation method thereof and detection methods.The Test paperBe made of substrate and colour developing indicator paper block, colour developing indicator paper block is pasted onto substrate one end, colour developing indicator paper block surface be attached with color developing agent andSurfactant；Color developing agent is the mixture of dimethyl diaminophenazine chloride and phenolphthalein.The technique effect that the present invention has is：
The Test paper can accurately detect water quality total alkalinity, i.e., the summation of carbanion and bicarbonate ion in water,Have the advantages that quick, easy, easy to operate, easy to carry and real-time control, is suitable for tap water, swimming-pool water, seawater, industryThe test of the multiple-quality waters sample such as sewage.
Description of the drawings
Fig. 1 shows color chart；
Fig. 2~4 show the testing result of the Test paper examination criteria solution of 3 batches.
Specific implementation mode
The invention discloses a kind of Test paper of water quality total alkalinity and preparation method thereof and detection method, art technologiesPersonnel can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that all similar replacements and changingDynamic apparent to those skilled in the art, they are considered as being included in the present invention.The present invention method and answerWith being described by preferred embodiment, related personnel can obviously not depart from the content of present invention, in spirit and scopeMethod described herein and application are modified or are suitably changed and combined, to realize and apply the technology of the present invention.
Material therefor, reagent in Test paper of water quality total alkalinity provided by the invention and preparation method thereof and detection methodIt is available on the market.
With reference to embodiment, the present invention is further explained：
Embodiment 1
(1) manufacturing process
It being made of substrate and the colour developing indicator paper block with test function for being pasted onto substrate front end, substrate is polythene strip,Base panel length is 10cm, and width is 1cm.The indicator paper block that develops the color is chromatographic test paper block, and colour developing indicator paper block area is 1cm2.Develop the color test paperBlock is impregnated of three kinds of dimethyl diaminophenazine chloride, phenolphthalein and polyvinylpyrrolidone drugs.
1) preparation of maceration extract：
Dimethyl diaminophenazine chloride 0.3g, phenolphthalein 0.5g and polyvinylpyrrolidone 5.0g are weighed, 500mL deionized waters and 500mL is addedIn absolute ethyl alcohol mixed liquor, ultrasonic agitation is dissolved to no precipitation, is uniformly mixed, spare.
2) preparation of test paper：
It takes neutral filter paper to immerse in above-mentioned liquid, is taken out after complete wetting 10s, be placed in forced air drying in 80 DEG C of baking ovens, it is completeAfter white drying, drying is kept in dark place.
(2) making of colour code
Standard solution preparation method：
First precision weighs in sodium hydroxide 100mg to 250mL volumetric flasks, adds deionized water constant volume, shakes up, and dilute successivelyThe gradient solution to 400mg/L, 200mg/L, 100mg/L, 50mg/L, 10mg/L is released to get standard solution.
(3) application method of this test paper
One piece of this test strips are taken, immerses in sample liquid and is taken out after 1s, get rid of surplus liquid, color is observed after 60s, with colour codeCard comparison, reads the value of total alkalinity.
(4) result judgement method
The colour developing the result is shown in Figure 1 of color chart, colour code are purplish red, dark red, deep yellow, orange, blood red successively.Color depth corresponds toTable is shown in Table 1：
1 test strips color chart color depth of table corresponds to table
From the above results, total alkalinity can be judged in the following way：
Test strips show blood red, then total alkalinity is 400mg/L；
Test strips show orange-yellow between blood red, then total alkalinity is 200~400mg/L；
Test strips show it is orange-yellow, then total alkalinity be 200mg/L；
Test strips show orange-yellow between buff, then total alkalinity is 100~200mg/L；
Test strips show buff, then total alkalinity is 100mg/L；
Test strips show peony between buff, then total alkalinity is 50~100mg/L；
Test strips show peony, then total alkalinity is 50mg/L；
Test strips show aubergine between peony, then total alkalinity is 10~50mg/L；
Test strips show aubergine, then total alkalinity is 10mg/L；
Test strips show it is deeper than aubergine, then total alkalinity be less than 10mg/L.
(5) detection example
The gradient solution of 10mg/L, 50mg/L, 100mg/L, 200mg/L, 400mg/L are prepared to get standard solution.UsingThe total alkalinity of test strips made from above-mentioned preparation method (3 repetitions) examination criteria solution.It is detected simultaneously using water quality total alkalinityInstrument (model:ZJD-2A the total alkalinity of the standard solution) is detected.
Test result is shown in Fig. 2-4, and result data is as follows：
2 total alkalinity test strips accuracy experimental data table of table
From the above results, the method for the present invention accurately can detect total alkalinity, it can be achieved that total alkalinity instant detection.
2 water sample detection of embodiment
The water sample taken at random is detected, test result is as follows：
3 total alkalinity ELISA test strip sample result of table
| Sample ID | Test paper detection method | Instrument detection method |
| Tap water | 10 or less | 0.0 |
| Wahaha Pure Water | 10 or less | 0.0 |
| Rainwater | 10 or less | 0.6 |
The results show that Test paper of the present invention can accurately detect water quality total alkalinity.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the artFor member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answeredIt is considered as protection scope of the present invention.