技术领域technical field
本发明属于医疗设备技术领域，尤其是涉及一种用于全自动核酸提取扩增诊断一体机的PCR模块。The invention belongs to the technical field of medical equipment, and in particular relates to a PCR module used in a fully automatic nucleic acid extraction, amplification and diagnosis all-in-one machine.
背景技术Background technique
在进行分子诊断时，目前市场上大多数分子诊断都是半自动仪器，需要进行人工放入提取好的核酸步骤，在通过仪器进行定量分析，使得增大了人工干预的所产生的测试误差，测试速度慢，人工放入提取好的核酸容易造成生物污染，而在全自动提取扩增分子诊断设备中，PCR基因扩增仪的温度控制也是难点，PCR基因扩增仪是一款用于提供模拟基因扩增时在各个阶段所需的温度设备，并增加低温储存和荧光检测等功能，适用于人类基因组工程学，法医学、肿瘤学、组织和群体生物学、古生物学、动物学、植物学等研究领域及病毒、肿瘤、遗传病等临床诊断领域的聚合酶链反应荧光定量检测。When conducting molecular diagnosis, most of the molecular diagnosis on the market are semi-automatic instruments, which need to be manually inserted into the extracted nucleic acid step, and quantitative analysis is performed through the instrument, which increases the test error caused by manual intervention. The speed is slow, and the manual insertion of the extracted nucleic acid is likely to cause biological contamination. In the fully automatic extraction and amplification molecular diagnostic equipment, the temperature control of the PCR gene amplification instrument is also difficult. The PCR gene amplification instrument is used to provide analog The temperature equipment required at various stages of gene amplification, and added functions such as low-temperature storage and fluorescence detection, are suitable for human genome engineering, forensic science, oncology, tissue and population biology, paleontology, zoology, botany, etc. Fluorescent quantitative detection of polymerase chain reaction in the field of research and clinical diagnosis of viruses, tumors, and genetic diseases.
在中国专利文献CN103160426B中，公开了一种PCR基因扩增仪模块机构，包括模块本体，还设置有与模块本体紧密连接的模块导热均板以及设置在模块导热均板上的多个传感器固定柱，所述的模块本体上设置有若干试管孔槽，试管孔槽的底部与试管孔槽开口方向相反的一侧设有若干个对应于试管孔槽的导热柱，所述的导热柱的端面与模块导热均板的板面贴压在一起；所述的导热柱为圆柱体结构，远离设置在模块导热均板下方的发热源的导热柱的直径大于发热源中心处的导热柱直径；所述的试管孔槽呈矩阵结构均布在模块本体上。In the Chinese patent document CN103160426B, a PCR gene amplification instrument module mechanism is disclosed, including a module body, a module heat conduction uniform plate closely connected with the module body, and a plurality of sensor fixing columns arranged on the module heat conduction uniform plate , the module body is provided with a number of test tube slots, the bottom of the test tube slot is opposite to the opening direction of the test tube slot, and a number of heat conduction columns corresponding to the test tube slots are provided, and the end faces of the heat conduction columns are in line with the test tube slots. The board surfaces of the module heat conduction uniform plate are pressed together; the heat conduction column is a cylindrical structure, and the diameter of the heat conduction column away from the heat source arranged under the module heat conduction uniform plate is greater than the diameter of the heat conduction column at the center of the heat source; The test tube slots are evenly distributed on the module body in a matrix structure.
但上述技术方案中的PCR基因扩增仪模块机构在具体使用过程中不是很理想，温度控制差，检测方式不够灵活。However, the PCR gene thermal cycler module mechanism in the above technical solution is not very ideal in the specific use process, the temperature control is poor, and the detection method is not flexible enough.
发明内容Contents of the invention
本发明要解决的技术问题是提供一种温度控制好，结构紧凑，检测方式灵活的用于全自动核酸提取扩增诊断一体机的PCR模块。The technical problem to be solved by the present invention is to provide a PCR module with good temperature control, compact structure, and flexible detection methods for an all-in-one machine for automatic nucleic acid extraction, amplification, and diagnosis.
为解决上述技术问题，本发明采用的技术方案是，该用于全自动核酸提取扩增诊断一体机的PCR模块具有盖板，所述盖板上开有若干个试剂孔；在所述盖板的下方设置有与所述试剂孔相对应的热座，在所述热座的底部安装有加热片以及扩增散热器；所述加热片位于所述热座和所述扩增散热器之间，所述扩增散热器与盖板之间用尼龙圆柱连接；所述热座接入有光纤。In order to solve the above-mentioned technical problems, the technical solution adopted in the present invention is that the PCR module used for the fully automatic nucleic acid extraction, amplification and diagnosis all-in-one machine has a cover plate, and several reagent holes are opened on the cover plate; A hot seat corresponding to the reagent well is provided below the bottom of the hot seat, and a heating sheet and an amplification radiator are installed at the bottom of the hot seat; the heating sheet is located between the hot seat and the amplification radiator , the amplification radiator and the cover plate are connected by a nylon cylinder; the hot seat is connected with an optical fiber.
上述结构的PCR模块，可以做到能够来样即检功能，检测方式灵活，热座接入有光纤，采用四色荧光技术，能同时检测样本的四种指标；采用了光纤传导技术，将原先硕大的PCR设备缩小至小小的PCR模块之中，结构紧凑；同时也做到每个样本单独检验，互不干扰；在热座的底部安装有加热片以及扩增散热器，加热片位于所述热座和所述扩增散热器之间，更加容易控制温度；每一个热座都有相对应的试剂孔，都能单独对试剂孔里的核酸进行加热，热座每个温度不一定相同，可以单独控制，且与相邻的试剂孔之间互不影响，单个热座每一点的温度是相同的，温度均匀分布，检测效果更佳；解决了一体机能单独对每个试剂进行加热扩增的难题，提高一体机的测试速度。The PCR module with the above-mentioned structure can realize the function of detecting samples as soon as they come, and the detection method is flexible. The hot seat is connected to an optical fiber, and adopts four-color fluorescence technology, which can detect four indicators of the sample at the same time; The huge PCR equipment is reduced to a small PCR module with a compact structure; at the same time, each sample is tested separately without interfering with each other; a heating plate and an amplification radiator are installed at the bottom of the hot seat, and the heating plate is located at the bottom of the hot seat. Between the hot seat and the amplification radiator, it is easier to control the temperature; each hot seat has a corresponding reagent well, which can individually heat the nucleic acid in the reagent well, and the temperature of each hot seat is not necessarily the same , can be controlled separately, and does not affect each other with adjacent reagent wells, the temperature of each point of a single hot seat is the same, the temperature is evenly distributed, and the detection effect is better; it solves the problem that the integrated machine can heat and expand each reagent separately Increased difficulty, improve the test speed of the machine.
进一步的改进在于，所述加热片内设置有导线；所述热座由中空圆柱孔构成，在所述热座的中间位置横向突出有插入孔，所述光纤由所述插入孔接入至所述热座中。A further improvement is that the heating sheet is provided with wires; the hot seat is composed of a hollow cylindrical hole, and an insertion hole protrudes laterally from the middle position of the hot seat, and the optical fiber is connected to the In the hot seat.
通过导线加热，可以用导线串连多个加热片，加热方式更加便捷；热座由中空圆柱孔构成，EP管可通过试剂孔放入所对应的热座内。Through wire heating, multiple heating sheets can be connected in series with wires, and the heating method is more convenient; the hot seat is composed of a hollow cylindrical hole, and the EP tube can be put into the corresponding hot seat through the reagent hole.
进一步的改进在于，所述盖板上开有16个试剂孔，对应的，所述热座也具有16个；所述试剂孔上设置有台阶。A further improvement is that 16 reagent holes are opened on the cover plate, correspondingly, the hot seat also has 16; steps are provided on the reagent holes.
多个试剂孔，检测数量多，试剂孔上设置有台阶，方便检测操作。With multiple reagent holes, the number of detections is large, and there are steps on the reagent holes to facilitate the detection operation.
进一步的改进在于，所述扩增散热器包括有安装所述加热片的上端面；所述上端面相对的两个侧边垂直向下延伸有第一散热片和第二散热片；所述第一散热片和第二散热片之间还设置有若干个内部散热片，相邻两个内部散热片之间具有散热间隙。A further improvement is that the amplification radiator includes an upper end surface on which the heating sheet is installed; a first cooling fin and a second cooling fin extend vertically downward on the two opposite sides of the upper end surface; Several internal heat sinks are also arranged between the first heat sink and the second heat sink, and there is a heat dissipation gap between two adjacent internal heat sinks.
通过散热片的结构方式，使得扩增散热器散热更加便捷，控制热座的温度更加容易实现，且结构简单，成本低；扩增散热器通过上端面与加热片连接，散热更直接。Through the structural mode of the heat sink, it is more convenient to dissipate heat from the amplified radiator, and it is easier to realize the temperature control of the hot seat, and the structure is simple and the cost is low; the amplified radiator is connected to the heating sheet through the upper end surface, and the heat dissipation is more direct.
进一步的改进在于，所述试剂孔均匀分布在所述盖板上；所述盖板上设置有与所述尼龙圆柱相配合连接的安装孔。A further improvement is that the reagent holes are evenly distributed on the cover plate; and the cover plate is provided with installation holes that fit and connect with the nylon cylinder.
采用尼龙圆柱连接，起到隔热作用；试剂孔均匀分布相互独立，与试剂孔相对应的热座也均匀分布。Nylon cylinders are used to connect to play a role of heat insulation; the reagent wells are evenly distributed and independent of each other, and the hot seats corresponding to the reagent wells are also evenly distributed.
进一步的改进在于，所述热座的底部还具有安装板，所述安装板与所述加热片直接连接。设置安装板的作用使得热座的受热更加均匀，温度控制更稳定。A further improvement is that the bottom of the hot seat also has a mounting plate, and the mounting plate is directly connected to the heating chip. The effect of setting the mounting plate makes the heating of the hot seat more uniform and the temperature control more stable.
进一步的改进在于，所述加热片固定在热座和扩增散热器之间且有隔热板包覆，防止热量散失。A further improvement is that the heating sheet is fixed between the thermal seat and the amplification radiator and is covered by a heat shield to prevent heat loss.
进一步的改进在于，所述扩增散热器用所述尼龙圆柱安装在每一个热座下部，这样不会影响热座的温度。A further improvement is that the amplification radiator is installed on the lower part of each hot seat with the nylon cylinder, so that the temperature of the hot seat will not be affected.
本发明要解决的另一个技术问题是提供一种不需要进行人工放入提取好的核酸，测试精度高，而且测试时间短的全自动核酸提取扩增诊断一体机。Another technical problem to be solved by the present invention is to provide a fully automatic nucleic acid extraction, amplification and diagnosis all-in-one machine that does not need to be manually inserted into the extracted nucleic acid, has high test accuracy and short test time.
为解决上述技术问题，本发明采用的技术方案是，该全自动核酸提取扩增诊断一体机包括有底座以及设置在所述底座上的支架，还包括有：In order to solve the above technical problems, the technical solution adopted by the present invention is that the fully automatic nucleic acid extraction, amplification and diagnosis all-in-one machine includes a base and a bracket arranged on the base, and also includes:
核酸提取模块，用于提纯核酸，且所述核酸提取模块设置有反应平台，反应杯放置在所述反应平台上；The nucleic acid extraction module is used to purify nucleic acid, and the nucleic acid extraction module is provided with a reaction platform, and the reaction cup is placed on the reaction platform;
样本存取机构，用于保存样本，所述样本存取机构位于所述核酸提取模块的侧面且置于所述底座上，所述样本存取机构设置有样本架；The sample access mechanism is used to store samples, the sample access mechanism is located on the side of the nucleic acid extraction module and placed on the base, and the sample access mechanism is provided with a sample rack;
机械臂机构，具有Y轴水平箱体，Z轴垂直箱体沿所述Y轴水平箱体做Y轴水平运动；采样头在所述Z轴垂直箱体上做上下运动；所述Y轴水平箱体与所述支架相连接；The mechanical arm mechanism has a Y-axis horizontal box, and the Z-axis vertical box moves Y-axis horizontally along the Y-axis horizontal box; the sampling head moves up and down on the Z-axis vertical box; the Y-axis horizontal The box body is connected with the bracket;
硬件安装模块，用于安装硬件，所述硬件安装模块设置在所述Y轴水平箱体与所述支架相连接位置处的下方；A hardware installation module is used to install hardware, and the hardware installation module is arranged below the position where the Y-axis horizontal box body is connected to the bracket;
PCR模块，用于检测样本，所述PCR模块设置在所述硬件安装模块一侧且位于所述支架下方；A PCR module, used to detect samples, the PCR module is arranged on one side of the hardware installation module and under the bracket;
医疗废弃物收纳箱，用于收纳医疗废弃物，包括有设置在所述样本存取机构和所述PCR模块之间的收纳箱，且安装于所述底座上；The medical waste storage box is used to store medical waste, including a storage box arranged between the sample access mechanism and the PCR module, and installed on the base;
试剂存储机构，用于存放试剂，设置在所述医疗废弃物收纳箱的上方；The reagent storage mechanism is used to store reagents and is arranged above the medical waste storage box;
反应杯进样机构，设置在所述核酸提取模块的前端，且位于所述底座上。The reaction cup sampling mechanism is arranged at the front end of the nucleic acid extraction module and is located on the base.
采用上述技术方案，将各个部件均整合在底座上形成整套设备，结构一体化，流水化操作，测试时间短，使用效果好，反应杯通过反应杯进样机构被推入至核酸提取模块中，利用机械臂机构从样本存取机构中取样本加入到反应杯包装耗材中的反应杯中，反应结束后，核酸由机械臂机构提取至试剂存储机构的EP管中，反应杯废弃物集中处理至医疗废弃物收纳箱中；机械臂机构再从试剂存储机构中将装有试剂的EP管提取至PCR模块进行扩增检测；整体模块采用封闭式模块化设计，杜绝了因核酸提取而造成的设备污染问题；采用机械臂机构转移核酸，杜绝了因模块间相互影响对于检测数据的影响；硬件安装模块将所有电路板集成于硬件模块之内，杜绝了外部的电磁干扰问题，从而杜绝了因为电磁干扰而造成的数据波动，测试精度高。Using the above technical solution, all components are integrated on the base to form a complete set of equipment, with integrated structure, streamlined operation, short test time and good use effect. The reaction cup is pushed into the nucleic acid extraction module through the reaction cup sampling mechanism. Use the robotic arm mechanism to take samples from the sample access mechanism and add them to the cuvettes in the cuvette packaging consumables. After the reaction, the nucleic acid is extracted by the robotic arm mechanism to the EP tube of the reagent storage mechanism, and the cuvette waste is disposed of in a centralized manner. In the medical waste storage box; the mechanical arm mechanism extracts the EP tubes containing the reagents from the reagent storage mechanism to the PCR module for amplification detection; the overall module adopts a closed modular design, which eliminates equipment damage caused by nucleic acid extraction. Contamination problem; the use of a robotic arm mechanism to transfer nucleic acids eliminates the impact of the interaction between modules on the detection data; the hardware installation module integrates all circuit boards into the hardware module, eliminating external electromagnetic interference problems, thereby eliminating the problem of electromagnetic interference Data fluctuations caused by interference, high test accuracy.
进一步的改进在于，所述核酸提取模块具有动板组件，所述动板组件由上动板以及与所述上动板配装的下动板构成；在所述动板组件的下方设置有反应平台和垃圾箱，所述反应平台位于所述动板组件的前端；所述反应平台的下方设置有拨杆组件，拨杆组件中具有将反应杯废弃物拨到所述垃圾箱中的拨杆；所述拨杆组件还具有拨杆滑轨，所述拨杆滑轨上安装有拨杆滑块，所述拨杆设置在拨杆滑块上。A further improvement is that the nucleic acid extraction module has a moving plate assembly, and the moving plate assembly is composed of an upper moving plate and a lower moving plate fitted with the upper moving plate; A platform and a dustbin, the reaction platform is located at the front end of the moving plate assembly; a lever assembly is arranged below the reaction platform, and the lever assembly has a lever for moving cuvette waste into the dustbin The lever assembly also has a lever slide rail, a lever slider is installed on the lever slide rail, and the lever is arranged on the lever slider.
反应结束后，将反应杯废弃物拨到所述垃圾箱中，再由垃圾箱周转至医疗废弃物收纳箱，方便快捷，过程无污染；拨杆滑块使得拨杆可以在X轴向移动，推进各个反应杯，在反应结束后，将反应杯废弃物拨到所述垃圾箱中。After the reaction is over, put the cuvette waste into the dustbin, and then transfer it from the dustbin to the medical waste storage box, which is convenient and quick, and the process is pollution-free; the lever slider allows the lever to move in the X axis, Advance each cuvette and, after the reaction is complete, tuck cuvette waste into the bin.
进一步的改进在于，所述动板组件还包括有上动板丝杆电机和下动板丝杆电机，所述上动板上安装的磁棒在所述上动板丝杆电机的驱动下带动其上的磁套做上下运动，所述下动板上安装的脱环在所述下动板丝杆电机的驱动下脱去所述磁棒上的磁套。A further improvement is that the moving plate assembly also includes an upper moving plate screw motor and a lower moving plate screw motor, and the magnetic bar installed on the upper moving plate is driven by the upper moving plate screw motor. The magnetic sleeve on it moves up and down, and the stripping ring installed on the lower moving plate is driven by the screw motor of the lower moving plate to take off the magnetic sleeve on the magnetic bar.
进一步的改进在于，所述反应平台包括有平台框体，所述平台框体有若干个反应框体，相邻两个反应框体之间有反应隔间，所述平台框体的前后两端均设置有盖板。A further improvement is that the reaction platform includes a platform frame, the platform frame has several reaction frames, there is a reaction compartment between two adjacent reaction frames, and the front and rear ends of the platform frame All are provided with a cover plate.
反应隔间使得位于反应框体内的反应杯有一定距离，避免了杜绝了因核酸提取而造成的污染问题；平台框体的前后两端均设置有盖板起到了防污染以及加固反应平台的作用。The reaction compartment makes the reaction cups located in the reaction frame have a certain distance, avoiding the pollution caused by nucleic acid extraction; the front and rear ends of the platform frame are equipped with cover plates to prevent pollution and reinforce the reaction platform. .
进一步的改进在于，所述样本存取机构由位于上半部分的样本框和位于下半部分的样本散热器构成；所述样本框上设置有样本盖板，由所述样本框和所述样本盖板围成有若干个相互独立的样本架容置腔；所述样本架放置在所述样本架容置腔内，在所述样本架容置腔的底部设置有滑轨；所述样本框的背面设置有带检测器的样本区控制电路板；所述样本散热器具有若干片相互间隔的制冷片。A further improvement is that the sample access mechanism is composed of a sample frame located in the upper part and a sample radiator located in the lower part; a sample cover plate is arranged on the sample frame, and the sample frame and the sample The cover plate is surrounded by several mutually independent sample rack accommodating cavities; the sample rack is placed in the sample rack accommodating cavity, and a slide rail is provided at the bottom of the sample rack accommodating cavity; the sample frame A sample area control circuit board with a detector is arranged on the back of the sample area; the sample radiator has several cooling plates spaced apart from each other.
将样本存取机构分为上下两部分，结构紧凑，下半部分设置成散热器结构，既充分发挥其功能，又不影响其他部件诸如机械臂机构的工作；通过滑轨将样本架推进样本架容置腔，方便快捷。The sample access mechanism is divided into upper and lower parts, with a compact structure, and the lower part is set as a radiator structure, which not only fully exerts its functions, but also does not affect the work of other components such as the mechanical arm mechanism; push the sample rack into the sample rack through the slide rail Accommodating cavity, convenient and fast.
进一步的改进在于，所述样本架上具有若干个试管套装孔，所述样本盖板上设置有与所述样本架上试管套装孔数量相同的存取口；所述样本架底部具有与所述滑轨相配合的滑轨槽；机械臂机构通过存取口抓取样本架上的样本。A further improvement is that the sample rack has several test tube set holes, and the sample cover plate is provided with the same number of access openings as the number of test tube set holes on the sample rack; the bottom of the sample rack has a The sliding rail groove matched with the sliding rail; the mechanical arm mechanism grabs the sample on the sample rack through the access opening.
进一步的改进在于，所述试剂存储机构与所述样本存取机构的结构是相同的。A further improvement is that the reagent storage mechanism and the sample access mechanism have the same structure.
进一步的改进在于，所述机械臂机构的Z轴垂直箱体外侧设置有采样丝杆电机，与设置在所述Z轴垂直箱体内的采样丝杆联动相连，所述采样丝杆上设置有采样头，所述采样头在所述采样丝杆电机的驱动下沿所述Z轴垂直箱体做上下运动；所述Y轴水平箱体的外侧设置有移动丝杆电机，与设置在所述Y轴水平箱体内的移动丝杆联动相连，所述Z轴垂直箱体上的滑块套装在所述移动丝杆上，所述滑块在移动丝杆电机的驱动下沿所述Y轴水平箱体做Y轴水平运动。A further improvement is that a sampling screw motor is arranged on the outside of the Z-axis vertical box of the mechanical arm mechanism, and is connected in linkage with the sampling screw installed in the Z-axis vertical box, and the sampling screw is provided with a sampling screw. The sampling head moves up and down along the Z-axis vertical box under the drive of the sampling screw motor; the outer side of the Y-axis horizontal box is provided with a moving screw motor, which is arranged on the Y-axis The moving screw rods in the Z-axis horizontal box are connected in linkage, the slider on the Z-axis vertical box is set on the moving screw rod, and the slider moves along the Y-axis horizontal box under the drive of the moving screw motor. The body moves horizontally along the Y axis.
进一步的改进在于，所述Y轴水平箱体三面镂空；且所述采样头通过夹紧件设置在所述采样丝杆上，所述夹紧件套装在所述采样丝杆上；通过采用夹紧件，可以对采样头进行更换调整，保证采样准确。A further improvement is that the three sides of the Y-axis horizontal box are hollowed out; and the sampling head is set on the sampling screw rod through a clamping piece, and the clamping piece is sleeved on the sampling screw rod; Fasteners, the sampling head can be replaced and adjusted to ensure accurate sampling.
进一步的改进在于，所述硬件安装模块由电磁屏蔽罩与散热板套装而成，且所述散热板上设置有若干片硬件安装板，所述硬件安装板上具有尼龙柱；所述散热板上有散热孔。A further improvement is that the hardware installation module is assembled by an electromagnetic shielding cover and a heat dissipation plate, and several pieces of hardware installation plates are arranged on the heat dissipation plate, and nylon columns are provided on the hardware installation plate; There are cooling holes.
电磁屏蔽罩可以把大部分的电磁屏蔽掉，将所有电路板集成于硬件模块之内，杜绝了外部的电磁干扰问题，从而杜绝了因为电磁干扰而造成的数据波动。The electromagnetic shield can shield most of the electromagnetic, and integrate all circuit boards into the hardware module, eliminating external electromagnetic interference problems, thereby eliminating data fluctuations caused by electromagnetic interference.
进一步的改进在于，所述医疗废弃物收纳箱还包括有反应杯废弃物传送机和收纳箱盒盖，所述反应杯废弃物传送机的端部下方有收纳口，所述收纳口设置在所述收纳箱盒盖上；在所述反应杯废弃物传送机的侧面且位于所述收纳箱盒盖上设置有替补头废弃物脱卸装置；所述医疗废弃物收纳箱还包括有收纳箱导轨，设置在所述底座上，所述收纳箱盒盖通过设置在底部的收纳箱导轨槽与所述收纳箱导轨配装；所述收纳箱盒盖的外侧还设置有把手。A further improvement is that the medical waste storage box also includes a cuvette waste conveyor and a storage box cover, and there is a storage opening under the end of the cuvette waste conveyor, and the storage opening is set at the On the cover of the storage box; on the side of the cuvette waste conveyor and on the cover of the storage box, a replacement head waste removal device is arranged; the medical waste storage box also includes a storage box guide rail, It is arranged on the base, and the storage box cover is fitted with the storage box guide rail through the storage box guide rail groove provided at the bottom; the outer side of the storage box cover is also provided with a handle.
进一步的改进在于，所述反应杯进样机构具有反应杯盒，所述反应杯盒通过进样支架安装在所述底座上；反应杯装配在反应杯包装耗材内，推入所述反应杯盒内；所述反应杯进样机构的一端还设置有进样电机，所述进样电机具有同步带轮，在所述反应杯进样机构的另一端，还设置有与所述同步带轮相配合的惰轮；缠绕在所述同步带轮和所述惰轮上的同步带，位于上方的那一段置于所述反应杯盒内，位于下方的那一段置于所述反应杯盒的外侧；在所述同步带上还设置有反应杯推板。A further improvement is that the reaction cup sampling mechanism has a reaction cup box, and the reaction cup box is installed on the base through the sample injection bracket; the reaction cup is assembled in the reaction cup packaging consumables, and pushed into the reaction cup box Inside; one end of the reaction cup sampling mechanism is also provided with a sampling motor, the sampling motor has a synchronous pulley, and at the other end of the cuvette sampling mechanism, it is also provided with a Cooperating idler pulley; the synchronous belt wound on the synchronous pulley and the idler pulley, the upper section is placed in the reaction cup box, and the lower section is placed outside the reaction cup box ; A cuvette push plate is also provided on the synchronous belt.
附图说明Description of drawings
下面结合附图和本发明的实施方式进一步详细说明：Below in conjunction with accompanying drawing and embodiment of the present invention further describe in detail:
图1是本发明全自动核酸提取扩增诊断一体机结构示意图；Fig. 1 is a schematic structural view of the automatic nucleic acid extraction, amplification and diagnosis all-in-one machine of the present invention;
图2是图1中的核酸提取模块结构示意图；Fig. 2 is the structural representation of the nucleic acid extraction module in Fig. 1;
图3是图1中的样本存取机构结构示意图；Fig. 3 is a schematic structural diagram of the sample access mechanism in Fig. 1;
图4是图1中的机械臂机构结构示意图；Fig. 4 is a structural schematic diagram of the mechanical arm mechanism in Fig. 1;
图5是图1中的硬件安装模块结构示意图；Fig. 5 is a schematic structural diagram of the hardware installation module in Fig. 1;
图6是图1中的PCR模块结构示意图；Fig. 6 is a schematic structural diagram of the PCR module in Fig. 1;
图7是图1中的医疗废弃物收纳箱结构示意图；Fig. 7 is a schematic structural diagram of the medical waste storage box in Fig. 1;
图8是图1中的试剂存储机构结构示意图；Fig. 8 is a schematic structural diagram of the reagent storage mechanism in Fig. 1;
图9是图1中的反应杯进样机构结构示意图；Fig. 9 is a schematic structural diagram of the cuvette sampling mechanism in Fig. 1;
图10是图6中PCR模块分解结构示意图；Fig. 10 is a schematic diagram of the decomposition structure of the PCR module in Fig. 6;
图11是图10中的部分结构分解示意图；Fig. 11 is a schematic exploded view of part of the structure in Fig. 10;
其中：1-核酸提取模块；101-上动板；102-下动板；103-上动板丝杆电机；104-垃圾箱；105-磁棒；106-脱环；107-拨杆；108-反应平台；109-动板组件；110-拨杆组件；111-拨杆滑轨；112-拨杆滑块；113-下动板丝杆电机；114-磁套；115-平台框体；116-反应框体；117-反应隔间；118-盖板；2-样本存取机构；201-样本区控制电路板；202-样本盖板；203-样本架；204-样本框；205-样本散热器；206-滑轨；207-样本架容置腔；208-制冷片；209-试管套装孔；210-存取口；211-滑轨槽；3-机械臂机构；301-Y轴水平箱体；302-滑块；303-采样丝杆电机；304-移动丝杆；305-移动丝杆电机；306-Z轴垂直箱体；307-采样头；308-采样丝杆309-夹紧件；4-硬件安装模块；401-电磁屏蔽罩；402-硬件安装板；403-尼龙柱；404-散热板；405-散热孔；5-PCR模块；501-盖板；502-热座；503-加热片；504-光纤；505-扩增散热器；506-试剂孔；507-尼龙圆柱；508-导线；509-插入孔；510-台阶；511-上端面；512-第一散热片；513-第二散热片；514-内部散热片；515-散热间隙；516-安装孔；517-安装板；6-医疗废弃物收纳箱；601-反应杯废弃物传送机；602-替补头废弃物脱卸装置；603-收纳箱盒盖；604-把手；605-收纳箱导轨；606-收纳口；607-收纳箱导轨槽；7-试剂存储机构；8-反应杯进样机构；801-反应杯；802-反应杯盒；803-反应杯包装耗材；804-惰轮；805-反应杯推板；806-同步带；807-同步带轮；808-进样电机；809-进样支架；9-底座；10-支架。Among them: 1-nucleic acid extraction module; 101-upper moving plate; 102-lower moving plate; 103-screw motor of upper moving plate; 104-garbage bin; -reaction platform; 109-moving plate assembly; 110-driving rod assembly; 111-driving rod slide rail; 112-driving rod slider; 113-lower moving plate screw motor; 114-magnetic sleeve; 115-platform frame; 116-reaction frame; 117-reaction compartment; 118-cover; 2-sample access mechanism; 201-sample area control circuit board; 202-sample cover; 203-sample rack; 204-sample frame; 205- Sample radiator; 206-sliding rail; 207-sample holder chamber; 208-refrigerating sheet; 209-test tube set hole; 210-access port; 211-sliding rail groove; Horizontal box; 302-slider; 303-sampling screw motor; 304-moving screw; 305-moving screw motor; 306-Z axis vertical box; 307-sampling head; 308-sampling screw 309-clip Fastener; 4-hardware installation module; 401-electromagnetic shield; 402-hardware installation plate; 403-nylon column; 404-heat dissipation plate; 405-heat dissipation hole; 5-PCR module; ;503-heating plate; 504-optical fiber; 505-amplification radiator; 506-reagent hole; 507-nylon cylinder; 508-wire; 509-insertion hole; 510-step; 513-second heat sink; 514-internal heat sink; 515-radiation gap; 516-installation hole; 517-installation plate; 6-medical waste storage box; 601-reaction cup waste conveyor; Head waste removal device; 603-storage box cover; 604-handle; 605-storage box guide rail; 606-storage opening; 607-storage box guide rail groove; 7-reagent storage mechanism; - reaction cup; 802- reaction cup box; 803- reaction cup packaging consumables; 804- idler; 805- reaction cup push plate; 806- timing belt; 807- timing pulley; Bracket; 9-base; 10-bracket.
具体实施方式Detailed ways
如图1所示，是本发明的全自动核酸提取扩增诊断一体机，包括有底座9以及设置在所述底座9上的支架10，还包括有：As shown in Figure 1, it is a fully automatic nucleic acid extraction and amplification diagnostic all-in-one machine of the present invention, including a base 9 and a bracket 10 arranged on the base 9, and also includes:
核酸提取模块1，用于提纯核酸，且所述核酸提取模块1设置有反应平台108，反应杯801放置在所述反应平台108上；The nucleic acid extraction module 1 is used to purify nucleic acid, and the nucleic acid extraction module 1 is provided with a reaction platform 108, and the cuvette 801 is placed on the reaction platform 108;
样本存取机构2，用于保存样本，所述样本存取机构2位于所述核酸提取模块1的侧面且置于所述底座9上，所述样本存取机构2设置有样本架203；The sample access mechanism 2 is used to store samples. The sample access mechanism 2 is located on the side of the nucleic acid extraction module 1 and placed on the base 9. The sample access mechanism 2 is provided with a sample rack 203;
机械臂机构3，具有Y轴水平箱体301，Z轴垂直箱体306沿所述Y轴水平箱体301做Y轴水平运动；采样头在所述Z轴垂直箱体306上做上下运动；所述Y轴水平箱体301与所述支架10相连接；The mechanical arm mechanism 3 has a Y-axis horizontal box body 301, and a Z-axis vertical box body 306 moves Y-axis horizontally along the Y-axis horizontal box body 301; the sampling head moves up and down on the Z-axis vertical box body 306; The Y-axis horizontal box 301 is connected to the bracket 10;
硬件安装模块4，用于安装硬件设备和/或者其他附属设施，所述硬件安装模块4设置在所述Y轴水平箱体301与所述支架10相连接位置处的下方；The hardware installation module 4 is used to install hardware equipment and/or other auxiliary facilities, and the hardware installation module 4 is arranged below the position where the Y-axis horizontal box 301 is connected to the bracket 10;
PCR模块5，用于检测样本，所述PCR模块5设置在所述硬件安装模块4一侧且位于所述支架10下方；A PCR module 5, used for detecting samples, the PCR module 5 is arranged on one side of the hardware installation module 4 and is located below the bracket 10;
医疗废弃物收纳箱6，用于收纳医疗废弃物，包括有设置在所述样本存取机构2和所述PCR模块5之间的收纳箱盒盖603，且安装于所述底座9上；The medical waste storage box 6 is used to store medical waste, including a storage box cover 603 arranged between the sample access mechanism 2 and the PCR module 5, and installed on the base 9;
试剂存储机构7，用于存放试剂，设置在所述医疗废弃物收纳箱6的上方；A reagent storage mechanism 7, used for storing reagents, is arranged above the medical waste storage box 6;
反应杯进样机构8，设置在所述核酸提取模块1的前端，且位于所述底座9上。The cuvette sampling mechanism 8 is arranged at the front end of the nucleic acid extraction module 1 and is located on the base 9 .
如图2所示，核酸提取模块1具有动板组件109，所述动板组件109由上动板101以及与所述上动板101配装的下动板102构成；在所述动板组件109的下方设置有反应平台108和垃圾箱104，所述反应平台108位于所述动板组件109的前端；所述反应平台108的下方设置有拨杆组件110，拨杆组件110中具有将反应杯废弃物拨到所述垃圾箱104中的拨杆107；所述拨杆组件110还具有拨杆滑轨111，所述拨杆滑轨111上安装有拨杆滑块112，所述拨杆107设置在拨杆滑块112上。As shown in Figure 2, the nucleic acid extraction module 1 has a moving plate assembly 109, and the moving plate assembly 109 is composed of an upper moving plate 101 and a lower moving plate 102 fitted with the upper moving plate 101; 109 is provided with a reaction platform 108 and a dustbin 104, the reaction platform 108 is located at the front end of the moving plate assembly 109; Cup waste is dialed to the driving lever 107 in the dustbin 104; the driving lever assembly 110 also has a driving lever slide rail 111, and a driving lever sliding block 112 is installed on the driving lever sliding rail 111, and the driving lever 107 is arranged on the lever slider 112.
动板组件109还包括有上动板丝杆电机103和下动板丝杆电机113，所述上动板101上安装的磁棒105在所述上动板丝杆电机103的驱动下带动其上的磁套114做上下运动，所述下动板102上安装的脱环106在所述动板丝杆电机113的驱动下脱去所述磁棒105上的磁套114；还可以这样的，动板组件由若干个相互独立的上动板以及若干个相互独立的下动板构成，一个上动板与一下动板相互对应，配合操作，保证了核酸提取操作的独立性和高效性；反应平台108包括有平台框体115，所述平台框体115有若干个反应框体116，相邻两个反应框体116之间有反应隔间117，所述平台框体115的前后两端均设置有盖板118。The moving plate assembly 109 also includes an upper moving plate screw motor 103 and a lower moving plate screw motor 113, and the magnetic bar 105 installed on the upper moving plate 101 is driven by the upper moving plate screw motor 103. The upper magnetic sleeve 114 moves up and down, and the stripping ring 106 installed on the lower moving plate 102 is driven by the moving plate screw motor 113 to remove the magnetic sleeve 114 on the magnetic rod 105; , the moving plate assembly is composed of several mutually independent upper moving plates and several mutually independent lower moving plates, one upper moving plate and the lower moving plate correspond to each other and cooperate with each other to ensure the independence and high efficiency of the nucleic acid extraction operation; Reaction platform 108 comprises platform frame body 115, and described platform frame body 115 has several reaction frame bodies 116, and reaction compartment 117 is arranged between adjacent two reaction frame bodies 116, and the front and rear ends of described platform frame body 115 Both are provided with a cover plate 118 .
如图3所示，样本存取机构2由位于上半部分的样本框204和位于下半部分的样本散热器205构成；所述样本框204上设置有样本盖板202，由所述样本框204和所述样本盖板202围成有若干个相互独立的样本架容置腔207；所述样本架203放置在所述样本架容置腔207内，在所述样本架容置腔207的底部设置有滑轨206；所述样本框204的背面设置有带检测器的样本区控制电路板201；所述样本散热器205具有若干片相互间隔的制冷片208；样本架203上具有若干个试管套装孔209，所述样本盖板202上设置有与所述样本架203上试管套装孔209数量相同的存取口210；所述样本架203底部具有与所述滑轨206相配合的滑轨槽211。As shown in Figure 3, the sample access mechanism 2 is composed of a sample frame 204 located in the upper part and a sample radiator 205 located in the lower part; the sample frame 204 is provided with a sample cover plate 202, and the sample frame 204 and the sample cover plate 202 enclose several mutually independent sample rack accommodating cavities 207; the sample rack 203 is placed in the sample rack accommodating cavity 207, and The bottom is provided with a slide rail 206; the back of the sample frame 204 is provided with a sample area control circuit board 201 with a detector; the sample radiator 205 has several cooling plates 208 spaced from each other; Test tube set holes 209, the sample cover plate 202 is provided with access openings 210 with the same number of test tube set holes 209 on the sample rack 203; Rail groove 211.
在本发明实施例中，试剂存储机构7与所述样本存取机构2的结构是相同的，如图8所示；该机构有试剂架放入EP管沿滑轨推进试剂框内，试剂区控制电路板带有检测器，可以感应是否有试剂进入，底部按有制冷片可使试剂始终处于冷藏状态。In the embodiment of the present invention, the structure of the reagent storage mechanism 7 is the same as that of the sample access mechanism 2, as shown in FIG. The control circuit board is equipped with a detector, which can sense whether there is reagent entering, and there is a refrigeration sheet on the bottom to keep the reagent in a refrigerated state.
如图4所示，所述机械臂机构3的Z轴垂直箱体306外侧设置有采样丝杆电机303，与设置在所述Z轴垂直箱体306内的采样丝杆308联动相连，所述采样丝杆308上设置有采样头307，所述采样头307在所述采样丝杆电机303的驱动下沿所述Z轴垂直箱体306做上下运动；所述Y轴水平箱体301的外侧设置有移动丝杆电机305，与设置在所述Y轴水平箱体301内的移动丝杆304联动相连，所述Z轴垂直箱体306上的滑块302套装在所述移动丝杆304上，所述滑块302在移动丝杆电机305的驱动下沿所述Y轴水平箱体301做Y轴水平运动。As shown in Figure 4, a sampling screw motor 303 is arranged outside the Z-axis vertical box 306 of the mechanical arm mechanism 3, and is connected in linkage with the sampling screw 308 arranged in the Z-axis vertical box 306. A sampling head 307 is arranged on the sampling screw 308, and the sampling head 307 moves up and down along the Z-axis vertical box 306 driven by the sampling screw motor 303; the outside of the Y-axis horizontal box 301 A moving screw motor 305 is provided, which is connected in linkage with the moving screw 304 arranged in the Y-axis horizontal box 301 , and the slider 302 on the Z-axis vertical box 306 is set on the moving screw 304 , the slider 302 moves horizontally along the Y-axis horizontally along the Y-axis horizontal box 301 under the drive of the moving screw motor 305 .
在本实施例中，Y轴水平箱体301三面镂空；且所述采样头307通过夹紧件309设置在所述采样丝杆308上，所述夹紧件309套装在所述采样丝杆308上。In this embodiment, the Y-axis horizontal box 301 is hollowed out on three sides; and the sampling head 307 is set on the sampling screw rod 308 through a clamping part 309, and the clamping part 309 is sleeved on the sampling screw rod 308 superior.
如图5所示，硬件安装模块4由电磁屏蔽罩401与散热板404套装而成，且所述散热板404上设置有若干片硬件安装板402，所述硬件安装板402上具有尼龙柱403；所述散热板404上有散热孔405。As shown in Figure 5, the hardware installation module 4 is made of an electromagnetic shielding cover 401 and a heat dissipation plate 404, and the heat dissipation plate 404 is provided with several hardware installation plates 402, and the hardware installation plate 402 has a nylon post 403 ; There are cooling holes 405 on the cooling plate 404 .
如图6所示，PCR模块5具有盖板501，所述盖板501上开有若干个试剂孔506；在所述盖板501的下方设置有与所述试剂孔506相对应的热座502，在所述热座502的底部安装有加热片503以及扩增散热器505；所述加热片503位于所述热座502和所述扩增散热器505之间，所述扩增散热器505与盖板501之间用尼龙圆柱507连接；所述热座502接入有光纤504。As shown in Figure 6, the PCR module 5 has a cover plate 501, and several reagent holes 506 are opened on the cover plate 501; a hot seat 502 corresponding to the reagent holes 506 is provided below the cover plate 501 , a heating plate 503 and an amplification radiator 505 are installed at the bottom of the thermal seat 502; the heating plate 503 is located between the thermal seat 502 and the amplification radiator 505, and the amplification radiator 505 The nylon cylinder 507 is used to connect with the cover plate 501; the thermal seat 502 is connected with an optical fiber 504.
如图10所示，是PCR模块5分解结构示意图，可以看到更多细节方面的结构，加热片503内设置有两根导线508；所述热座502由中空圆柱孔构成，在所述热座502的中间位置横向突出有插入孔509，所述光纤504由所述插入孔509接入至所述热座502中，在本实施例中，所述盖板501上开有16个试剂孔506，对应的，所述热座502也具有16个；所述试剂孔506上设置有台阶510，所述扩增散热器505包括有安装所述加热片503的上端面511；所述上端面511相对的两个侧边垂直向下延伸有第一散热片512和第二散热片513；所述第一散热片512和第二散热片513之间还设置有若干个内部散热片514，相邻两个内部散热片514之间具有散热间隙515，所述试剂孔506均匀分布在所述盖板501上；所述盖板501上设置有与所述尼龙圆柱507相配合连接的安装孔516，所述热座502的底部还具有安装板517，所述安装板517与所述加热片503直接连接；所述加热片503固定在热座502和扩增散热器505之间且有隔热板(未示出)包覆；扩增散热器505用所述尼龙圆柱507安装在每一个热座502下部；图11中可见位于上方的EP管插入至试剂孔506。As shown in Figure 10, it is a schematic diagram of the decomposition structure of the PCR module 5, and the structure in more details can be seen. Two wires 508 are arranged in the heating sheet 503; An insertion hole 509 protrudes laterally from the middle position of the seat 502, and the optical fiber 504 is inserted into the hot seat 502 through the insertion hole 509. In this embodiment, 16 reagent holes are opened on the cover plate 501 506, correspondingly, the hot seat 502 also has 16; the reagent hole 506 is provided with a step 510, and the amplification radiator 505 includes an upper end surface 511 on which the heating sheet 503 is installed; the upper end surface The two opposite sides of 511 extend vertically downwards with a first cooling fin 512 and a second cooling fin 513; between the first cooling fin 512 and the second cooling fin 513, a number of internal cooling fins 514 are arranged, corresponding to There is a heat dissipation gap 515 between two adjacent internal cooling fins 514, and the reagent holes 506 are evenly distributed on the cover plate 501; the cover plate 501 is provided with a mounting hole 516 that is matched with the nylon cylinder 507 , the bottom of the hot seat 502 also has a mounting plate 517, the mounting plate 517 is directly connected to the heating sheet 503; the heating sheet 503 is fixed between the hot seat 502 and the amplification radiator 505 and has heat insulation The plate (not shown) is covered; the amplification radiator 505 is installed on the lower part of each heat seat 502 with the nylon cylinder 507; the EP tube located above is inserted into the reagent hole 506 as seen in FIG. 11 .
如图7中所示，所述医疗废弃物收纳箱6还包括有反应杯废弃物传送机601和收纳箱盒盖603，所述反应杯废弃物传送机601的端部下方有收纳口606，所述收纳口606设置在所述收纳箱盒盖603上；在所述反应杯废弃物传送机601的侧面且位于所述收纳箱盒盖603上设置有替补头废弃物脱卸装置602；所述医疗废弃物收纳箱6还包括有收纳箱导轨605，设置在所述底座9上，所述收纳箱盒盖603通过设置在底部的收纳箱导轨槽607与所述收纳箱导轨605配装；所述收纳箱盒盖603的外侧还设置有把手604。As shown in FIG. 7 , the medical waste storage box 6 further includes a cuvette waste conveyor 601 and a storage box cover 603 , and a storage opening 606 is provided under the end of the cuvette waste conveyor 601 , The storage opening 606 is set on the storage box cover 603; on the side of the cuvette waste conveyor 601 and on the storage box cover 603, a replacement head waste removal device 602 is set; the The medical waste storage box 6 also includes a storage box guide rail 605, which is arranged on the base 9, and the storage box cover 603 is fitted with the storage box guide rail 605 through the storage box guide rail groove 607 arranged at the bottom; A handle 604 is also provided on the outside of the storage box cover 603 .
如图9所示，反应杯进样机构8具有反应杯盒802，所述反应杯盒802通过进样支架809安装在所述底座9上；反应杯801装配在反应杯包装耗材803内，推入所述反应杯盒802内；所述反应杯进样机构8的一端还设置有进样电机808，所述进样电机808具有同步带轮807，在所述反应杯进样机构8的另一端，还设置有与所述同步带轮807相配合的惰轮804；缠绕在所述同步带轮808和所述惰轮804上的同步带806，位于上方的那一段置于所述反应杯盒802内，位于下方的那一段置于所述反应杯盒802的外侧；在所述同步带806上还设置有反应杯推板805，作为变动，还可以的，在同步带的上下两侧均设置有反应杯推板，这样，能够节约反应杯包装耗材的推进时间。As shown in Figure 9, the reaction cup sampling mechanism 8 has a reaction cup box 802, and the reaction cup box 802 is installed on the base 9 through a sample injection bracket 809; the reaction cup 801 is assembled in the reaction cup packaging consumable 803, pushed into the cuvette box 802; one end of the cuvette sampling mechanism 8 is also provided with a sampling motor 808, the sampling motor 808 has a synchronous pulley 807, and at the other end of the cuvette sampling mechanism 8 One end is also provided with an idler pulley 804 matched with the timing pulley 807; a timing belt 806 wound on the timing pulley 808 and the idler pulley 804, the upper part is placed in the cuvette In the box 802, the lower section is placed on the outside of the cuvette box 802; a cuvette push plate 805 is also provided on the timing belt 806. As a change, it is also possible to place it on the upper and lower sides of the timing belt Both are provided with cuvette push plates, so that the time for advancing cuvette packaging consumables can be saved.
具体使用时，反应杯801装配在反应杯包装耗材803中，如图9所示，进样电机808动作，带动同步带轮807，同步带806转动，同步带806上的反应杯推板805移动，反应杯包装耗材803装在反应杯包装耗材盒811内，手动推入反应杯盒802内；反应杯801被反应杯推板805推入至核酸提取模块1中，如图2所示，拨杆107将反应杯801拨至反应框体116中；利用机械臂机构3从样本存取机构2中取样本加入到反应杯801中，机械臂机构3在支架10上沿X轴移动，可调整X轴向位置；反应结束后，核酸由机械臂机构3提取至试剂存储机构7中，拨杆107把反应杯废弃物拨到垃圾箱104内，垃圾箱104内的反应杯废弃物通过图7中所示的反应杯废弃物传送机601传送至医疗废弃物收纳箱6中的收纳箱盒盖内，旁边还有替补头废弃物脱卸装置602，将替补头脱落至收纳箱盒盖内；机械臂机构3再从试剂存储机构7中将装有试剂的EP管提取至PCR模块5进行检测，检测系统采用光纤传导技术，将原先硕大的PCR设备缩小至小小的PCR模块之中，同时也做到每个样本单独检验，互不干扰。During specific use, the cuvette 801 is assembled in the cuvette packaging consumable 803, as shown in Figure 9, the sample injection motor 808 moves, drives the timing pulley 807, the timing belt 806 rotates, and the cuvette push plate 805 on the timing belt 806 moves , the reaction cup packaging consumables 803 are installed in the reaction cup packaging consumables box 811, and manually pushed into the reaction cup box 802; the reaction cup 801 is pushed into the nucleic acid extraction module 1 by the reaction cup push plate 805, as shown in Figure 2, dial The rod 107 dials the cuvette 801 into the reaction frame 116; the mechanical arm mechanism 3 is used to take a sample from the sample access mechanism 2 and add it to the cuvette 801, and the mechanical arm mechanism 3 moves along the X-axis on the support 10 and can be adjusted X-axis position; after the reaction, the nucleic acid is extracted by the mechanical arm mechanism 3 into the reagent storage mechanism 7, and the lever 107 moves the cuvette waste into the garbage bin 104, and the cuvette waste in the garbage bin 104 passes through as shown in Figure 7 The cuvette waste conveyor 601 shown in is sent to the storage box cover in the medical waste storage box 6, and there is a replacement head waste removal device 602 next to it, which will drop the replacement head into the storage box cover; The arm mechanism 3 extracts the EP tube containing the reagent from the reagent storage mechanism 7 to the PCR module 5 for detection. The detection system uses optical fiber transmission technology to reduce the original huge PCR equipment to a small PCR module. Each sample is tested separately without interfering with each other.
以上显示和描述了本发明的基本原理、主要特征及优点。本行业的技术人员应该了解，本发明不受上述实施例的限制，上述实施例和说明书中描述的只是说明本发明的原理，在不脱离本发明精神和范围的前提下，本发明还会有各种变化和改进，例如对于安装在底座上各个部件的位置做一些调整，这些变化和改进都落入要求保护的本发明范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。The basic principles, main features and advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Various changes and improvements, such as some adjustments to the positions of the various components installed on the base, all fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810510781.7ACN108456640A (en) | 2018-05-24 | 2018-05-24 | The PCR modules of diagnosis all-in-one machine are expanded for full-automatic nucleic acid extraction |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810510781.7ACN108456640A (en) | 2018-05-24 | 2018-05-24 | The PCR modules of diagnosis all-in-one machine are expanded for full-automatic nucleic acid extraction |
| Publication Number | Publication Date |
|---|---|
| CN108456640Atrue CN108456640A (en) | 2018-08-28 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810510781.7APendingCN108456640A (en) | 2018-05-24 | 2018-05-24 | The PCR modules of diagnosis all-in-one machine are expanded for full-automatic nucleic acid extraction |
| Country | Link |
|---|---|
| CN (1) | CN108456640A (en) |
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|---|---|---|---|---|
| CN113373043A (en)* | 2021-06-09 | 2021-09-10 | 北京卓诚惠生生物科技股份有限公司 | Thermal cracking temperature control device |
| CN115895854A (en)* | 2021-09-30 | 2023-04-04 | 北京威妙生物科技有限公司 | Multi-card compatible molecular diagnostic system |
| CN117286025A (en)* | 2023-09-13 | 2023-12-26 | 艾普拜生物科技(苏州)有限公司 | Temperature control unit for PCR instrument |
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| CN208414441U (en)* | 2018-05-24 | 2019-01-22 | 南京岚煜生物科技有限公司 | The PCR module of diagnosis all-in-one machine is expanded for full-automatic nucleic acid extraction |
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| US20050106612A1 (en)* | 2002-01-28 | 2005-05-19 | Varouj Amirkhanian | Integrated bio-analysis and sample preparation system |
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| US20140038192A1 (en)* | 2012-07-31 | 2014-02-06 | Gen-Probe Incorporated | System, method, and apparatus for automated incubation |
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| CN113373043A (en)* | 2021-06-09 | 2021-09-10 | 北京卓诚惠生生物科技股份有限公司 | Thermal cracking temperature control device |
| CN115895854A (en)* | 2021-09-30 | 2023-04-04 | 北京威妙生物科技有限公司 | Multi-card compatible molecular diagnostic system |
| CN117286025A (en)* | 2023-09-13 | 2023-12-26 | 艾普拜生物科技(苏州)有限公司 | Temperature control unit for PCR instrument |
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