A kind of magnetotactic bacteria culture instrumentTechnical field
The invention belongs to Bacteria Culture technical field, specifically a kind of magnetotactic bacteria culture instrument.
Background technology
Magnetotactic bacteria, which is one kind, can make directed movement under the action of external magnetic field and form nano magnetic particle-in vivoThe bacterium of magnetic corpusculum is distributed mainly in soil, lake and ocean.
With biomedical rapid development, bacteriological incubator is widely used in field of biology, but environmental factorVery big on the culture of bacterium influence, requirement of each bacterium to culture environment is all different, and the requirement of some bacteriums is very high veryIt as harshness, varies slightly and just easilys lead to culture failure, many obstructions are brought to work such as experiment, researchs.At present existingWhen growing directly from seeds living, the inconvenient germ culturing device easily used, when needing Bacteria Culture, generally use conventional bacteria culture dressIt sets, it is difficult to sufficiently effective mixing be carried out to inoculum, the culture efficiency of bacterium is reduced, influence Bacteria Culture effect.ThisOutside, the progress for also influencing enterprise work, certain work difficulty is increased to staff.
In view of the above-mentioned problems, a Chinese patent application No. is CN201520296190.6 discloses a kind of Bacteria CultureCase, include Bacteria Culture box body and place inoculum teat glass, the left inside wall of the Bacteria Culture box body andRight inner wall is equipped with crossbeam symmetrically, and the crossbeam is equipped with groove, and the teat glass frame is described on the grooveTemperature-controlling component, timing bench, pressure adjusting knob and data display screen are provided on the right outer wall of incubator box body, which can essenceReally temperature, pressure, humidity, the illumination etc. in control incubator box body, moreover it is possible to control the time of Bacteria Culture, improve Bacteria CultureSuccess rate, the fostering requirement of most of bacterium can be met.But the apparatus structure is complicated, and cannot be to the bacterium in incubatorCulture solution is renewed, and has certain limitation.
In consideration of it, a kind of magnetotactic bacteria culture instrument of the present invention, by the way that upper layer liquid-feeding tube and lower layer's liquid-feeding tube is arrangedInternal channel promotes magnetotactic bacteria flourish process, it can be achieved that hold the replacement of magnetotactic bacteria culture solution in ware.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of magnetotactic bacteria culture instrument, present invention is mainly used forThe culture of magnetotactic bacteria.The mutual cooperation that the present invention shakes module and clamp module by box house makes magnetotactic bacteria bacterium solution fillPoint mixing, is conducive to the reproductive efficiency for improving magnetotactic bacteria, meanwhile, change liquid by be arranged that liquid feeding module can realize box houseProcess improves the working efficiency of equipment.
The technical solution adopted by the present invention to solve the technical problems is：A kind of magnetotactic bacteria culture of the present inventionInstrument, including babinet, case lid, shaking module, clamp module and liquid feeding module, the babinet are rectangle uncovered babinet, babinetLeft and right sidewall symmetrically opens up left groove and right groove；The case lid is horizontally arranged at top of the box；The shaking module setting existsThe inner bottom of babinet shakes module for making magnetotactic bacteria rock to promote incubation；The clamp module settingIn the top for shaking module, the device that clamp module is used to hold magnetotactic bacteria clamps, and improves device stability when shaking；InstituteIt states liquid feeding module to be arranged in the top of clamp module, liquid feeding module is used to that magnetotactic bacteria culture solution to be added with to becoming to box houseMagnetic bacterium is cultivated.
The shaking module includes take-up motor, take-up spring, roller, steel wire rope, support spring and holds ware, the caseTake-up motor is installed in the left groove of body, take-up spring is horizontally mounted in right groove；The roller is placed in babinet bottomThe heart, opens up No.1 groove on roller, and lay winding wire ropes in No.1 groove, one end of steel wire rope is connect with take-up motor, steel wire ropeThe other end connect with take-up spring；The support spring is connected to the bottom of box vertically, and support spring is arranged two；The ShengIt puts ware to be placed on roller, it is arc to hold ware outer bottom, and inside is planar structure, holds ware both ends and the top of support springPortion connects, and the inner horizontal for holding ware opens up No.1 sliding slot.When work, take-up motor is opened, take-up motor pulls steel wire rope, steelCord drives roller to move left and right, and roller, which moves left and right drive and holds ware, to be moved left and right.
The clamp module includes idler wheel, holder, electromagnet, No.1 spring and baffle, is installed in the No.1 sliding slotIdler wheel, vertical connecting bracket on idler wheel, holder are L-type, and holder is arranged two；The internal stent level opens up No. two grooves,Electromagnet is set in No. two grooves, and the other end of electromagnet horizontal connection No.1 spring, No.1 spring connects baffle.WorkWhen, electromagnet is by driving No.1 spring that baffle is driven to keep out holder on the side wall of No.1 sliding slot.
The liquid feeding module includes culture dish, magnetic patch, upper layer liquid-feeding tube, gear, liquid feeding motor, lower layer's liquid-feeding tube and toothItem, the holder centre connect culture dish, and culture dish is rectangle uncovered babinet；The magnetic patch is mounted on the side wall of culture dishIn；The upper layer liquid-feeding tube is vertically installed on case lid, and upper layer liquid-feeding tube is cylindrical shape, and the bottom of upper layer liquid-feeding tube passes through case lidPositioned at box house, the lower half portion surface of upper layer liquid-feeding tube opens up sliding slot；The gear and the setting of liquid feeding motor add on upper layerInside the entity of liquid pipe；Lower layer's liquid-feeding tube, which is arranged inside the bottom of upper layer liquid-feeding tube, lower layer's liquid-feeding tube, installs rack, toothItem is meshed with gear, and channel is opened up inside upper layer liquid-feeding tube and lower layer's liquid-feeding tube.When work, liquid feeding motor, liquid feeding electricity are openedMachine is rotated by sliding tooth wheel, gear by band carry-over bar to drive lower layer's liquid-feeding tube to be moved upwardly or downwardly along sliding slot, it is realThe merging and separation of existing upper layer liquid-feeding tube and lower layer's liquid-feeding tube, can be under when there is liquid to be flowed into from the center of upper layer liquid-feeding tubeThe center of layer liquid-feeding tube is flowed into culture dish, and when needing to change liquid, the center of upper layer liquid-feeding tube and lower layer's liquid-feeding tube is blocked,Liquid is set to be flowed out from channel.
The support level connects No. two springs, and the other end of No. two springs connects fixture block, and fixture block connects with culture dish side wallIt connects.It is more steady that the setting of No. two springs and fixture block makes culture dish be positioned to, and improves stability when equipment work.
The culture dish bottom opens up semicircular arc groove about central symmetry, and partition board is arranged in culture dish, and partition board is hingedOn the side wall of semicircular arc groove, vertical connection No.1 spring, the No.1 spring other end and semicircular arc groove below partition boardBottom connects；Electromagnet is set inside the culture dish entity.When work, when needing to change liquid inside culture dish, pass through controlSwitch opens electromagnet, and electromagnet attracts partition board to move downward, and magnetotactic bacteria enters in semicircular arc groove, becomes when preventing from changing liquidMagnetic bacterium goes out from upper layer liquid-feeding tube and lower layer's liquid-feeding tube, changes after the completion of liquid, disconnects control switch, and electromagnet loses magnetism, becomesMagnetic bacterium returns in culture dish, and partition board sets back in the effect of No.1 spring.
The beneficial effects of the invention are as follows：
1. a kind of magnetotactic bacteria culture instrument of the present invention, the present invention is added by the way that liquid feeding module is arranged into culture dishInoculum, then the mutual cooperation by shaking module and clamp module, keep the magnetotactic bacteria culture solution in culture dish abundantMixing, accelerates the culture rate of magnetotactic bacteria.
2. a kind of magnetotactic bacteria culture instrument of the present invention, the present invention is by being arranged liquid feeding mould upper layer liquid-feeding tube in the blockExternal sliding slot makes lower layer's liquid-feeding tube that can be slided up and down along upper layer liquid-feeding tube, it can be achieved that magnetotactic bacteria in culture dish changesLiquid improves the service efficiency of equipment and the proliferative speed of magnetotactic bacteria.
3. a kind of magnetotactic bacteria culture instrument of the present invention, the present invention by be arranged No. two springs connected on holder andFixture block makes culture dish be positioned to more steady, improves stability when equipment is shaken, extend the service life of equipment.
4. a kind of magnetotactic bacteria culture instrument of the present invention, the present invention is recessed by the semicircular arc that culture dish bottom is arrangedSlot, when carrying out changing liquid, unlatching electromagnet makes partition board be moved to semicircular arc inside grooves, attraction of the magnetotactic bacteria in electromagnetEffect is lower to be entered in semicircular arc groove, and magnetotactic bacteria is sucked away when preventing from changing liquid, is changed liquid and is disconnected electromagnet after the completion, magnetotactic is thinBacterium comes back in culture dish, and partition board restores horizontal under the action of No.1 spring, improves the culture efficiency of magnetotactic bacteria.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is that holder of the present invention is mounted on the sectional view held in ware；
Fig. 3 is partial enlarged view at the A of Fig. 1；
Fig. 4 is partial enlarged view at the B of Fig. 1；
Fig. 5 is the left view of roller of the present invention；
In figure：Babinet 1, case lid 2, shake module 3, clamp module 4, liquid feeding module 5, take-up motor 31, take-up spring 32,Roller 33, support spring 35, holds ware 36, idler wheel 41, holder 42, electromagnet 43, No.1 spring 44, baffle at steel wire rope 3445, culture dish 51, magnetic patch 52, upper layer liquid-feeding tube 53, gear 54,56, No. two springs 6 of lower layer's liquid-feeding tube, fixture block 7, partition board 8, oneNumber spring 9, electromagnet 10.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie belowSpecific implementation mode is closed, the present invention is further explained.
As shown in Figures 1 to 5, a kind of magnetotactic bacteria culture instrument of the present invention includes babinet 1, case lid 2, shakes module3, clamp module 4 and liquid feeding module 5, the babinet 1 are rectangle uncovered babinet, and the left and right sidewall of babinet 1 symmetrically opens up left recessedSlot 11 and right groove 12；The case lid 2 is horizontally arranged at 1 top of babinet；The inner bottom in babinet 1 is arranged in the shaking module 3Layer shakes module 3 for making magnetotactic bacteria rock to promote incubation；Module 3 is being shaken in the setting of the clamp module 4Top, clamp module 4 be used for will hold magnetotactic bacteria device clamp, improve shake when device stability；The liquid feeding mouldBlock 5 is arranged in the top of clamp module 4, and liquid feeding module 5 is used to the inside of babinet 1 be added magnetotactic bacteria culture solution with thin to magnetotacticBacterium is cultivated.
The shaking module 3 includes take-up motor 31, take-up spring 32, roller 33, steel wire rope 34, support spring 35 and containsWare 36 is put, take-up motor 31 is installed in the left groove 11 of the babinet 1, take-up spring 32 is horizontally mounted in right groove 12；It is describedRoller 33 is placed on the center of 1 bottom of babinet, opens up No.1 groove on roller 33, lay winding wire ropes 34 in No.1 groove, steel wireOne end of rope 34 is connect with take-up motor 31, and the other end of steel wire rope 34 is connect with take-up spring 32；The support spring 35 is perpendicularDirect-connected to be connected on 1 bottom of babinet, support spring 35 is arranged two；The ware 36 that holds is placed on roller 33, holds 36 outer layer of wareBottom is arc, and inside is planar structure, holds 36 both ends of ware and is connect with the top of support spring 35, holds the internal water of ware 36It is opened flat and sets No.1 sliding slot.When work, take-up motor 31 is opened, take-up motor 31 pulls steel wire rope 34, steel wire rope 34 to drive roller33 move left and right, and roller 33, which moves left and right drive and holds ware 36, to be moved left and right.
The clamp module 4 includes idler wheel 41, holder 42, electromagnet 43, No.1 spring 44 and baffle 45, the No.1Idler wheel 41 is installed, vertical connecting bracket 42 on idler wheel 41, holder 42 is L-type, and holder 42 is arranged two in sliding slot；The holder 42Inner horizontal opens up No. two grooves, and electromagnet 43,43 horizontal connection No.1 spring 44 of electromagnet, No.1 bullet is arranged in No. two groovesThe other end of spring 44 connects baffle 45.When work, electromagnet 43 is by driving No.1 spring 44 to drive baffle 45 by holder42 keep out on the side wall of No.1 sliding slot.
The liquid feeding module 5 includes culture dish 51, magnetic patch 52, upper layer liquid-feeding tube 53, gear 54, liquid feeding motor 55, lower layerLiquid-feeding tube 56 and rack 57,42 centre of the holder connect culture dish 51, and culture dish 51 is rectangle uncovered babinet；The magnetic patch52 are mounted in the side wall of culture dish 51；The upper layer liquid-feeding tube 53 is vertically installed on case lid 2, and upper layer liquid-feeding tube 53 is cylinderThe bottom of shape, upper layer liquid-feeding tube 53 is located at across case lid 2 inside babinet 1, and the lower half portion surface of upper layer liquid-feeding tube 53 opens up cunningSlot；The gear 54 and liquid feeding motor 55 are arranged inside the entity of upper layer liquid-feeding tube 53；The setting of lower layer's liquid-feeding tube 56 existsRack 57 is installed in the bottom of upper layer liquid-feeding tube 53,56 inside of lower layer's liquid-feeding tube, and rack 57 is meshed with gear 54, upper layer liquid-feeding tube53 and lower layer's liquid-feeding tube 56 inside open up channel.When work, liquid feeding motor 55 is opened, liquid feeding motor 55 is by driving 54 turns of gearDynamic, gear 54, to drive lower layer's liquid-feeding tube 56 to be moved upwardly or downwardly along sliding slot, realizes upper layer liquid feeding by band carry-over bar 57The merging and separation of pipe 53 and lower layer's liquid-feeding tube 56 can add when there is liquid to be flowed into from the center of upper layer liquid-feeding tube 53 along lower layerThe center of liquid pipe 56 is flowed into culture dish 51, when needing to change liquid, by the center of upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56It blocks, liquid is made to be flowed out from channel.
The other end of the holder No. two springs 6 of 42 horizontal connection, No. two springs 6 connects fixture block 7, fixture block 7 and culture dish51 side walls connect.It is more steady that the setting of No. two springs 6 and fixture block 7 makes culture dish 51 be positioned to, and improves steady when equipment worksIt is qualitative.
51 bottom of the culture dish opens up semicircular arc groove about central symmetry, and partition board 8, partition board is arranged in culture dish 518 are hinged on the side wall of semicircular arc groove, the vertical connection No.1 spring 9 in 8 lower section of partition board, 9 other end of No.1 spring and semicircleArc-shaped groove bottom connects；Electromagnet 10 is set inside 51 entity of the culture dish.When work, need to change inside culture dish 51When liquid, electromagnet 10 is opened by controlling to switch, electromagnet 10 attracts partition board to move downward, and it is recessed that magnetotactic bacteria enters semicircular arcIn slot, magnetotactic bacteria is gone out from upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 when preventing from changing liquid, is changed after the completion of liquid, is disconnected control and is openedIt closes, electromagnet 10 loses magnetism, and magnetotactic bacteria returns in culture dish 51, and partition board 8 sets back in the effect of No.1 spring 9.
When work, electromagnet 43 is first opened, electromagnet 43 is by driving No.1 spring 44 to drive baffle 45 by holder 42It keeps out on the side wall of No.1 sliding slot.
Open liquid feeding motor 55, liquid feeding motor 55 by driving gear 54 to rotate, gear 54 by band carry-over bar 57 toIt drives lower layer liquid-feeding tube 56 to be moved downward along sliding slot, realizes the separation of upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56, liquid is from upperThe center of layer liquid-feeding tube 53 is flowed into along the center of lower layer's liquid-feeding tube 56 in culture dish 51 after flowing into.
So that upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 is merged by controlling liquid feeding motor 55 again, open take-up motor 31,Take-up motor 31 pulls steel wire rope 34, steel wire rope 34 that roller 33 is driven to move left and right, and roller 33 moves left and right drive and holds ware 36It moves left and right, culture dish 51 follows to hold ware 36 and move left and right and carries out rocking mixing to the culture solution in culture dish 51, accelerates to becomeThe incubation of magnetic bacterium.
When needing to change liquid, upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 are separated, then by upper layer liquid-feeding tube 53 underThe center of layer liquid-feeding tube 56 is blocked, and opens electromagnet 10 by controlling to switch, electromagnet 10 attracts partition board to move downward, and magnetotactic is thinBacterium enters in semicircular arc groove, and magnetotactic bacteria is gone out from upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 when preventing from changing liquid, cultureChannel outflow of the culture solution inside upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 in ware 51 carries out liquid replacement, changes liquid completionAfterwards, control switch is disconnected, electromagnet 10 loses magnetism, and magnetotactic bacteria returns in culture dish 51, work of the partition board 8 in No.1 spring 9With setting back.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry shouldUnderstand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present inventionReason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvementsIt all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circleIt is fixed.