A kind of composite natral sweetener compositionTechnical field
One kind that the present invention relates to a kind of for replacing sugar, artificial sweetener and simple natural sweetener natural flaw is multipleNatural sweetener composition is closed, present invention relates particularly to acidity regulator and flavoring agent is used, by reducing prolonging for sugariness generationLate and/or the sugariness residence time of sweetener is reduced to improve the taste of natural sweetener.
Background technology
In recent years, diabetes problem is on the rise and the continuous improvement of people's health care consciousness, and consumer is to low sugar and nothingThe demand of sugared healthy food is increasing.Sweetener especially natural sweetener is added in food can be made with substituting sucroseAt low sugar and sugarfree foods, there is auxiliary curative effect to the treatment of the diseases such as diabetes, hypertension, cardiovascular disease, and with onlySpecial physiological function is the trend of current nutrient health development.
Steviol glycoside is the natural health type sweetener that essence carries from catananche's STEVIA REBAUDIANA and medical auxiliary treatmentAgent.It has the characteristics that high sugariness, low in calories, and sugariness is 200-450 times of sucrose, is a kind of ideal sucrose succedaneum,It is referred to as " the big sugar source in third place in the world ".And Rebaudioside A is the sweet ingredient that mouthfeel is best in stevioside, cool taste canMouthful, without rear bitter taste, it can preferably improve the taste matter of food.
Momordia grosvenori aglycone is the extract for originating in endemic plants from Guangxi Siraitia grosvenorii, and sugariness is 300 times of sucrose, safe nothingPoison is the natural sweetener of a new generation.It is similar to glycyrrhizin, there is eliminating phlegm and relieving cough, anti-inflammatory, antibacterial, anti-oxidant, hypoglycemic, guarantorThe physiological functions such as liver.
Radix Glycyrrhizae sweetener is the sweet ingredient extracted from the root, stem of glycyrrhizic legume, is a kind of natural sweet tasteAgent.Its sugariness is 200-300 times of sucrose, low in calories, is that U.S.'s food is included in medication management in " generally acknowledged safe material "One of the sweetener of most sweet tea, ADI is without regulation.And Glycyrrhetic acid 3-O-mono-BETA-D-glucuronide (GAMG) be with glycyrrhizic acid (GL) be bottomObject obtains glycyrrhizin of new generation by the directionally hydrolyzing technology of GRD beta-glucuronidase, and sugariness is more than 900 times of sucrose.
Compared with sucrose, to occur that relatively slow, the sweet taste residence time is longer etc. naturally scarce there are sweet taste for simple natural sweetenerIt falls into.Such as Rebaudioside A, its sugariness time of origin is slightly later than sucrose；The rear sweet tea sense of glycyrrhizic acid is stronger, and the sugariness residence time is long；The distinctive Siraitia grosvenorii pleasant impression of Mogroside -V is heavier.
Invention content
Goal of the invention：The present invention provides one kind to be used in various products, and overcomes natural sweetener sweet taste specialThe composite natral sweetener composition of property defect.
Technical solution：A kind of composite natral sweetener composition of the present invention, including at least one high-potency sweetener, extremelyA kind of few acidity regulator, at least one flavoring agent and at least one nutritive sweetener；The high-potency sweetener and acidity tuneSection agent mass ratio is 4-10:1；The high-potency sweetener is 2-10 with flavoring agent mass ratio:1；The high-potency sweetener and nutritionProperty sweetener mass ratio be 0.01-1:1.
Preferably：The mass ratio of high-potency sweetener and acidity regulator is 5:1；The mass ratio of high-potency sweetener and flavoring agentFor from 5:1；The mass ratio of high-potency sweetener and nutritive sweetener is 0.25:1.
The high-potency sweetener, acidity regulator, flavoring agent and nutritive sweetener mass ratio are：5:1:1:20.
The high-potency sweetener includes that mass ratio is 15-27：2-5：3-7：Steviol glycoside and stevia extract, sieve of 1-3Chinese berry extract, glycyrrhizic acid and Glycyrrhetic acid 3-O-mono-BETA-D-glucuronide and its salt and/or solvate.
The steviol glycoside and stevia extract include Rebaudioside A.
Further, the quality purity of qualities of stevia extract Rebaudioside A is 95%-99%.
Further, the quality purity of qualities of stevia extract Rebaudioside A is 99%.
The Fructus Monordicae extract includes Fructus Monordicae extract saponin V, is preferred with purity height.
Further, the quality purity of Fructus Monordicae extract saponin V is 25%-50%.
Further, the quality purity of Fructus Monordicae extract saponin V is 50%.
The glycyrrhizic acid and glycyrrhizin derivative and its salt and/or solvate include single glucuronic acid Radix Glycyrrhizae timeAcid, (GAMG) is with glycyrrhizic acid (GL) for substrate, and by the directionally hydrolyzing technology of GRD beta-glucuronidase, it is sweet to obtain a new generationCareless sweetener, shown in structure such as formula (I)：
Further, steviol glycoside and stevia extract, Fructus Monordicae extract, glycyrrhizic acid and single glucuronic acid Radix Glycyrrhizae timeAcid and its salt and/or solvate mass ratio are 21:3.5:5:2.
The acidity regulator is selected from citric acid and its salt, malic acid, fumaric acid, tartaric acid, phosphoric acid and its salt and grapeIt is one or more in sodium saccharate.
The flavoring agent is one or more in glycine, l-Alanine and sodium glutamate.
Preferably, flavoring agent is glycine.
The nutritive sweetener is selected from D-sorbite, antierythrite, xylitol, isomaltoketose, maltitol and sweetReveal one or more in glycan.
The composition can be configured to syrup, powder type, tablet form, particle or solution.
Addition acidity regulator and flavoring agent can solve the problems, such as associated with natural high potency sweetener in above-mentioned composition,Especially sweet taste occurs compared with slow and sweet taste residence time longer problem.Therefore, sweetener composition of the invention and containingStating the product of composition logical can provide more satisfying sweetness perception time graph.
Unless otherwise indicated, " % " indicates mass percentage in the present invention.
As used herein, term " time graph " is to weigh the sweetness intensities perceived at any time.It is satisfactory or haveThe time graph of power is that can experience sweet taste rapidly and with one section of curve of the relatively short duration similar to sucrose.
Advantageous effect：(1) stevia extract of the invention, Fructus Monordicae extract, glycyrrhizic acid and single glucuronic acid Radix GlycyrrhizaeThere is hypo acid (GAMG) sweet taste synergy, such blend to have desired taste profile in high-potency sweetener component,Mouthfeel is close to sucrose；(2) composite natral sweetener composition of the present invention is in good taste, sugariness is high, is similar to sucrose, sweet teaIt is short that the very fast and sweet taste residence time occurs for taste, with heat under sucrose or fructose equal sweetness is relatively low or even empty calory, can be withInstead of the use of nutritive sweeteners, there are the physiological functions such as anti-inflammatory, anticancer, anti-caries tooth.
Specific implementation mode
One, raw material sources
Rebaudioside A purity is 95-99%, comes from the Shandong bio tech ltd Sheng Xiangyuan；
Fructus Monordicae extract saponin V purity is 25-50%, comes from Hunan Hua Cheng living resources limited liability company；
Glycyrrhetic acid 3-O-mono-BETA-D-glucuronide (GAMG) comes from hundred million Li Nai sparrows bio tech ltd；
Remaining material is commercially available gained.
Two, composite natral sweetener composition is prepared
2.1 preparation method
Composite natral sweetener composition preparation method is：Take high-potency sweetener (the auspicious Bao of stevia extract of formula ratioEnlightening glycosides A, Fructus Monordicae extract saponin V, glycyrrhizic acid, Glycyrrhetic acid 3-O-mono-BETA-D-glucuronide (GAMG)), acidity regulator (citric acid,Sodium citrate and malic acid) and flavoring agent (glycine) respectively after mixing press equal increments method be added antierythrite, sorbSugar alcohol and xylitol after mixing well, crushed 60 mesh sieve to get compound sweetener composition.
2.2 sample preparation
Embodiment 1：Composite natral sweetener composition：21g purity be 98% stevia extract Rebaudioside A, 2g it is pureDegree is 25% Fructus Monordicae extract saponin V, 5g glycyrrhizic acid, 2g Glycyrrhetic acid 3-O-mono-BETA-D-glucuronides (GAMG), 5g malic acid, 5gGlycine, 40g antierythrites, 20g D-sorbites, 20g xylitols.
Embodiment 2：Composite natral sweetener composition：25g purity be 98% stevia extract Rebaudioside A, 5g it is pureDegree is 50% Fructus Monordicae extract saponin V, 4g glycyrrhizic acid, 1g Glycyrrhetic acid 3-O-mono-BETA-D-glucuronides (GAMG), 5g malic acid, 2gNatrium malicum, 7g glycine, 40g antierythrites, 30g D-sorbites, 30g xylitols.
Embodiment 3：Composite natral sweetener composition：21g purity be 99% stevia extract Rebaudioside A, 3.5gPurity be 50% Fructus Monordicae extract saponin V, 5g glycyrrhizic acid, 2g Glycyrrhetic acid 3-O-mono-BETA-D-glucuronides (GAMG), 5g citric acids,5g sodium citrates, 4g malic acid, 4g glycine, 40g antierythrites, 20g D-sorbites, 20g xylitols.
Embodiment 4：Composite natral sweetener composition：27g purity be 96% stevia extract Rebaudioside A, 5g it is pureDegree is 40% Fructus Monordicae extract saponin V, 7g glycyrrhizic acid, 3g Glycyrrhetic acid 3-O-mono-BETA-D-glucuronides (GAMG), 2g citric acids, 4gSodium citrate, 2g malic acid, 8g glycine, 30g antierythrites, 30g D-sorbites, 30g xylitols.
Embodiment 5：Composite natral sweetener composition：21g purity be 99% stevia extract Rebaudioside A, 3.5gPurity be 50% Fructus Monordicae extract saponin V, 5g glycyrrhizic acid, 2g Glycyrrhetic acid 3-O-mono-BETA-D-glucuronides (GAMG), 2g citric acids,3g sodium citrates, 1.3g malic acid, 6.3g glycine, 40g antierythrites, 46g D-sorbites, 40g xylitols.
Comparative example 1：Without stevia extract Rebaudioside A, remaining is the same as embodiment 5.
Comparative example 2：Without Fructus Monordicae extract saponin V, remaining is the same as embodiment 5.
Comparative example 3：Without glycyrrhizic acid, remaining is the same as embodiment 5.
Comparative example 4：Without Glycyrrhetic acid 3-O-mono-BETA-D-glucuronide (GAMG), remaining is the same as embodiment 5.
Comparative example 5：Without stevia extract Rebaudioside A and Fructus Monordicae extract saponin V, remaining is the same as embodiment 5.
Comparative example 6：Without glycine, remaining is the same as embodiment 5.
Three, test method
The indices of sweetener are determined by tasting method, on the basis of sucrose, according to the judgement side on the basis of sucroseMethod, reference can be made to《GB 8270-2014》.Specific detection method is as follows in the present invention：
The index of sweet taste is dissolved in water on the basis of 2% aqueous sucrose solution, by different samples with different concentration,When the level of sweetness presented when sample is in a certain concentration is just identical with standard sucrose aqueous solution, the sample is recordedMass concentration, mass concentration is identical as 2% sucrose concentration to be defined as 1, as sample mass concentration often increases by onePercentage point, then reduce with sucrose similitude, and the series of evaluation also accordingly increases.
The measurement of bitter taste, using caffeine solution as standard solution, nest's alcohol product that wherein mass fraction is 0.05% are blackSolution on the basis of coffee, concentration often increase by 0.05%, and rating increases by 1 grade, assay method of the specific method with sweet taste.
Peculiar smell is using 0.005% Fructus Monordicae extract saponin V as standard solution, and concentration often increases by 0.0025%, ratingIncrease by 2 grades, assay method of the specific method with sweet taste.
Sugariness indicates entry into sweetness perception in oral cavity to the speed of sweet taste, and the sucrose solution with mass fraction 2% isStandard, it is determined as 1 grade, remaining is 3 grades for 0.002% lactose and 0.5% Sionit solution, and 0.002% lactose solution is 5 grades,Specific method is the same as sweet taste assay method.
Sweet taste demurrage enters intraoral extinction time as standard using sweet taste, and the mass concentration of sample is 2%, whenBetween section be 5-15s, 5s be 1 grade, it is incremented by successively, be divided into 5 grades.
In order to ensure the accurate fixed and result correctness and reproducibility of measurement of each criticism person to each sample, Mei GepingSurvey person should repeat 3-5 times the measurement of each sample, and the time interval that each sample measures is more than 5 minutes, and each sampleAfter product measure, oral cavity is cleaned with clear water.
Embodiment 1-5 carries out sweetener group by one group of profession sense organ evaluation and test person (per test group 9 or 12 test and appraisal persons)Close the test and appraisal of object sweet taste characteristic.Following characteristic is to be assessed on 1 to 5 in specification：
1) similar to sucrose, (1=is similar to sucrose；5=is not similar to sucrose completely)；
2) (1=is bitter not at all for bitter taste；5=is extremely bitter)；
3) (1=does not have peculiar smell to peculiar smell；5=peculiar smell is extremely heavy)
4) (1=is extremely slow for sugariness generation；5=is exceedingly fast)；
5) sweet taste demurrage, (1=was extremely short；5=is extremely long).
3.2 test result
The sweet taste characteristic evaluating result of the different sweeteners of table 1
The quality of sweetener is using sucrose as standard, and the sweet taste characteristic of sweetener is similar to sucrose, and the sweet taste residence timeIt is short, and the sweet taste residence time is the important indicator of sweetener, and very formidable difficult point in the prior art, sweet taste is in oral cavityThe interior residence time is long, can generate bad impression to sense organ.In addition, sweetener also requires bitter taste and peculiar smell smaller, it is of the inventionComponent improves the generation of bitter taste and peculiar smell in the disadvantage for overcoming prior art sweet taste demurrage length.
By stevia extract Rebaudioside A it can be seen from comparative example 1, comparative example 2 and comparative example 5 and 5 result of embodiment andFructus Monordicae extract saponin V two kinds of components influences overall performance of product, and glycine affects mouthfeel and the reduction of sampleThe bitter taste of product.These results display the present invention composite sweetener composition mouthfeel be similar to sucrose, it is more not bitter, be no differentTaste occurs with faster sugariness and with shorter sweet taste demurrage, achieves the entirety that existing product is unable to reachEffect.