Invention content
An object of the present invention is to provide a kind of preparation method of aerosol type suspending agent and its answering on coccidial vaccineWith, it is of the invention to have and be used simultaneously with vaccine, it is easy to use, it is cheap, the features such as easily storage；Droplet of the present invention exists150um or so, 100 chickens of spraying, which are immunized, to be needed 1 second, and primary immunization ensures that droplet is uniform throughout one's life, easy to operate, saves peopleWork, large-scale cultivation company preferably and hatchery use；The invention of aerosol type suspending agent can be to avoid coccidial vaccine drinking-water bandPhenomenon is exempted from the various emergent and leakage come, ensures the uniformity that coccidial vaccine is immunized；And the oral toxic side effect to body is reduced,Popularization for coccidial vaccine offers convenience condition.
The second object of the present invention is to a kind of preparation method of aerosol type suspending agent is provided and its on coccidial vaccine shouldWith
The present invention is achieved through the following technical solutions：
A kind of preparation method of aerosol type suspending agent and its application on coccidial vaccine, invention component and its parts by weightCounting ratio is：
Moreover, the component of the aerosol type suspending agent and its optimal ratio of weight and number are：
A kind of the step of preparation method of aerosol type suspending agent, preparation method is：
(1) weigh purified water respectively according to weight proportion to be put into Agitation Tank, sequentially add glycerine, sorbierite, stirredIt mixes to 25 minutes, surveys density and meet the requirements, it is spare；
(2) oligofructose, hydroxypropyl methyl cellulose are sequentially added in above-mentioned solution (1), stirred 30 minutes, until solutionThere is wire drawing phenomenon, it is spare；
(3) above-mentioned solution (2) is adjusted into PH to neutrality, uniformly mixing 15 minutes, filtered to get to a kind of aerosol type suspendingThe finished product of agent.
Advantages of the present invention and advantageous effect are：
1st, the present invention can be used with vaccine simultaneously, easy to use, cheap, easily be stored；
2nd, droplet of the present invention needs 1 second in 150um or so, immune 100 chickens of spraying, and primary immunization ensures throughout one's life, mistDrop is uniform, easy to operate, saves artificial, large-scale cultivation company preferably and hatchery use；
3rd, the various emergent and leakage that the invention of aerosol type suspending agent of the present invention can bring to avoid coccidial vaccine drinking-water is exempted from existingAs ensureing the uniformity that coccidial vaccine is immunized.
Embodiment 3
A kind of preparation method of aerosol type suspending agent and its application on coccidial vaccine, the component of feed addictive andIts weight is respectively：10 parts of glycerine, 10 parts of hydroxypropyl methyl cellulose, 15 parts of oligofructose, 5 parts of sorbierite, purified water 60Part.
The step of preparation method, is the same as embodiment 1.
Clinical practice
1. stability test test result analysis table in aqueous solution：
Experiment description：
Equipment and reagent used take drinking water 1000ml, egg capsule mixed liquor 1ml, every milliliter containing 1,500,000, egg capsule (heap-type,Huge, tender, murder by poisoning mixture), instrument biomicroscope, counter, tally, 1ml syringes, beaker, graduated cylinder, glassGlass stick；
Test procedure：It is first put into beaker with graduated cylinder amount 1000ml water, pipetting 1ml egg capsule liquid with syringe is put into beakerIn, then the present invention is added to according to operation instruction in beaker and is stirred until homogeneous, is then poured into graduated graduated cylinder by stirringIn, point is detected the test of the egg capsule quantity egg capsule uniformity in different times respectively
Conclusion：Different time points, the ratio of egg capsule quantity illustrate the non-convention of suspending effect of the present invention within the scope ofThink, and suspending effect is up to 9 hours；The inhomogenous spraying immune brought of levels egg capsule will not be caused because of spray time is longerIt is inhomogenous.
2. effect detects
1000ml is watered according to 5 bottles of (2000 plumage parts/bottle) coccidial vaccines, 100ml of the present invention is added in, is configured to coccidial vaccineSuspension is put spare in beaker；It is detected according to normal immune efficacy detection method, sees aerosol type suspending agent whether to coccidian oocystImmune definite value has noiseless；
With 3-7 age in days SPF chickens 30, it is divided into three groups, 1 group of 10 chicken is not inoculated with to be observed as blank；2 groups of 10 chickens are through heightAtomizer machine is pressed to carry out immunoprophylaxis；Every 1 plumage part of chicken；1 plumage part of every chicken of 3 groups of 10 chicken oral vaccinations；Three groups of chickens are distinguishedIsolated rearing at identical conditions.The chicken (2-3 groups) of 21 ages in days, the coccidial vaccine of every chicken inoculation Zheng Dian company after inoculation(containing E.acervulina 100,000, other 3 kinds respectively containing 5 ten thousand) to observation 5-7 days, all chickens of dissect, the disease of every chicken of inspectionBecome and score, control group at least 9 chicken duodenums, small intestine interrupt, caecum lesion is scored should all >=﹢ 3.0 divides；Immune group is at least8 chicken duodenums, mid small bowel, caecum lesion score should all≤﹢ 1.0 divides.(droplet is in 150um, pressure 6-7) record.
Test result analysis table
Conclusion：Experimental data in above table, the results showed that attack the immune guarantor of poison to the immune chicken after oral vaccinationShield rate is 99%；Suspending agent does not influence definite value of the coccidian oocyst in chicken body；Spraying immune protective rate is 100%, in time saving provinceImmune effect is more prominent on the basis of power.
Attached score sheet：
Lesion caused by 1.1 Eimeria tenellas, (both sides caecum lesion is or not dissect caecum between 5-7 days after infectionWhen consistent, it is subject to serious side).
0 point is visible by naked eyes lesion.
﹢ 1 divides cecal content normal, and intestinal mucosa is visible to be dispersed in blutpunkte or blood spots on a small quantity.
﹢ 2 divides cecal content band blood, and intestinal wall facial mask face blutpunkte or blood spots quantity are more intensive, and intestinal wall thickens；
﹢ 3 divides caecum silght enlargement, is thickened in enteric cavity without normal cecal feces, intestinal mucosa, and caecum intracavitary is full of darkThere is caecum core in red blood (in the cheesy Banana Type block of canescence)；
﹢ 4 divides caecum heavy swelling, and caecum intracavitary does not have caecum excrement or has been enclosed in intestines core, solidifying full of necrosis in enteric cavityClot and cecum mucosa fragment.It scores because of the lethal chicken of this coccidia and divides for ﹢ 4.
Dissect small intestine between 5-7 days after lesion infection caused by 1.2 Eimeria Necatrixes.
0 point is visible by naked eyes lesion
﹢ 1 divides the mid small bowel mucosal surface blutpunkte that visible tip-like size is dispersed in and white point, visible a small amount of Exocarpium Citri Rubrum in enteric cavityColor content, intestinal wall slightly thicken.
2 points of intestinal walls of ﹢ thicken, and serosal surface has a large amount of blutpunkte and white point, and small intestine, which interrupts, slight flatulence occurs, enteric cavity partIt is mixed with sludged blood.
﹢ 3 divides, and serosal surface has a large amount of blutpunkte and white point, and slight flatulence occurs in mid small bowel, and enteric cavity is locally mixed with blood coagulationBlock
﹢ 4 divides mid small bowel height swelling, and intestinal segment atrophy occurs, is obviously shortened, after lesion extends to duodenum and small intestineSection, color of soy sauce or brown mucus, intestinal mucosa bleeding, necrosis are contained in small intestine contents.Because the lethal chicken of this coccidia is denoted as ﹢ 4Point.
Lesion caused by 1.3 Eimeria maximas, dissect small intestine between 5-7 days after infection.
0 point is visible by naked eyes lesion.
﹢ 1 divides mid small bowel to occur the visible smaller blutpunkte of mucosal surface, having a small amount of crocus mucus in enteric cavity.
﹢ 2 divides mid small bowel slight flatulence occur, and the visible many blutpunktes of mucosal surface extend to duodenum, can in enteric cavitySee a large amount of crocus mucus.
﹢ 3 divides dilatation of intestine, and without normal intestinal contents, intestinal contents are mixed with blood clot and crocus mucus, and intestinal wall becomesThin, mucosal surface is coarse.
﹢ 4 divides intestinal tube swelling, and intestinal contents are sticky, in rufous, and is mixed with a large amount of blood clots, washes meat water colour in light, intestines glueFilm is congested, and bleeding comes off.It scores because of the lethal chicken of this coccidia and divides for ﹢ 4.
Lesion caused by 1.4 heap-type Eimerias, dissect duodenum between 5-7 days after infection.
0 point is visible by naked eyes lesion.
﹢ 1 divides duodenal serosa face and mucosal surface are visible to have the striation hickie being dispersed in, and transversely arranged, outer light is presentedIt is trapezoidal.
﹢ 2 divides lesion more intensive, but does not merge, and duodenum facial mask is in coated with transversely arranged striation hickie, appearanceExisting scalariform, lesion extend to jejunum, and alimentary canal content is leaner, and intestinal mucosa thickens.
﹢ 3 divides duodenal mucosa visible intensive canescence lesion, and lesion is extended at yolk cyst wall, and enteron aisle is pale, intestinesIntracavitary has volume moisture, and content is in watery liquid.
﹢ 4 divides duodenal mucosa, and in light gray, striation hickie merges completely, full of butyrous exudate, intestines in intestinal tubeWall thickening, because the lethal chicken of this coccidia ﹢ 4 that scores divides.