The content of the invention
The technical problem to be solved in the present invention is existing PCR instrument when being reacted, and staff is needed specialComputer beside wait, other affairs can not be handled, especially go out relevant affairs, so as to provide a kind of intelligence PCRInstrument.
An aspect of of the present present invention provides a kind of intelligent PCR instrument, including：Housing；Thermocirculator is arranged on describedIn housing, for carrying out temperature conditionss to PCR process；Fluorescence detection device is arranged in the housing, is usedIn to the progress fluoroscopic examination of PCR result；Master board is arranged in the housing, with the thermocirculatorIt is electrically connected with the fluorescence detection device；Multiple interfaces, set on the housing, for connect exterior terminal and with instituteIt states exterior terminal to be communicated, the multiple interface includes following at least two：RJ-45 interfaces, RJ-11 interface, SC optical fiber connectMouth, fddi interface, AUI interfaces, bnc interface, Console interfaces, USB interface；Interface convertor is arranged on the master boardOn, it is electrically connected with the multiple interface, it is any in the multiple interface for the data of the master board to be converted intoThe information that a interface can transmit；Wireless communication module is arranged in the master board, for realizing nothing with exterior terminalLine communicates.
Optionally, the wireless communication module includes at least one of：WIFI module, bluetooth module, Zigbee module,Mobile communication module.
Optionally, the mobile communication module includes：Gsm module, GPRS module, 3G module, 4G modules.
Optionally, the exterior terminal is one of the following：Router, desktop computer, mobile phone, tablet computer, notebook electricityBrain.
Optionally, the thermocirculator includes：Orifice plate, for placing sample tube；Heating module is arranged on the holeBelow plate, it is connected with the master board, for being heated or being cooled down；Hot cover is arranged on the sample tube,It is contacted with the sample tube mouth.
Optionally, further include：Sub-control plate is connected with the hot cover, for controlling the hot cover.
Optionally, further include：Master controller is arranged in the master board, and the master controller passes through the master controlInterface in making sheet is electrically connected with the thermocirculator and the fluorescence detection device, for controlling polymerase chain reactionThe process answered；Submaster controller is arranged in the master board, with the master controller, the wireless communication module and instituteIt states multiple interfaces to be electrically connected, for handling the communication with exterior terminal.
Optionally, further include：Power detecting module is electrically connected with the master controller, for detecting power supply unit outputElectrical energy parameter；Shine detection module, is electrically connected with the master controller, for detecting the luminous ginseng of the fluorescence detection deviceNumber；Temperature sensor is electrically connected with the master controller, for detecting the temperature of the heating module；Hot lid temperature sensor,It is electrically connected with the master controller, for detecting the temperature of the hot cover.
Optionally, the power detecting module, the luminous detection module, the temperature sensor and the hot lid temperatureSensor is electrically connected respectively with the submaster controller, and the submaster controller is used for the power detecting module, the luminous inspectionSurvey module, the testing result of the temperature sensor and the hot lid temperature sensor by the wireless communication module and/orThe multiple interface is sent to exterior terminal.
In the embodiment of the present invention, multiple interfaces are integrated in PCR instrument, can there is multiple and different species, the interface of signal, withSuitable for various exterior terminal interfaces, in this way, staff can be connected using various types of terminals with PCR instrument, without makingWith dedicated computer, you can it is monitored, also, by adding wireless communication module, can realize wireless monitor,As long as exterior terminal establishes wireless connection with PCR instrument, staff can anywhere realize the monitoring to PCR processes,Greatly improve convenience and the intelligence of PCR instrument.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with attached drawing, it is clear that described implementationExample is part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skillPersonnel's all other embodiments obtained without making creative work, belong to the scope of protection of the invention.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",The orientation or position relationship of the instructions such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely toConvenient for the description present invention and simplify description rather than instruction or imply signified device or element must have specific orientation,With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second "," the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phaseEven ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or be integrally connected；It canTo be mechanical connection or be electrically connected；It can be directly connected, can also be indirectly connected by intermediary, it can be withIt is the connection inside two elements, can is wireless connection or wired connection.For those of ordinary skill in the artFor, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each otherIt can be combined with each other into conflict.
The present embodiment provides a kind of intelligent PCR instrument, as shown in Figure 1, the intelligence PCR instrument includes：Housing 10, thermal cycleDevice 20, fluorescence detection device 30, master board 40, multiple interfaces 50, interface convertor 60 and wireless communication module 70.
Thermocirculator 20 is arranged in housing 10, for carrying out temperature conditionss to PCR process；FluorescenceDetection device 30 is arranged in housing 10, for carrying out fluoroscopic examination to PCR result；Master board 40 is setIn housing 10, it is electrically connected with thermocirculator 20 and fluorescence detection device 30；Multiple interfaces 50 are set on the housing 10,For connecting exterior terminal and being communicated with exterior terminal, multiple interfaces 50 include following at least two：RJ-45 interfaces, RJ-11 interfaces, SC optical fiber interfaces, fddi interface, AUI interfaces, bnc interface, Console interfaces, USB interface；Interface convertor 60 is setIt puts in master board 40, is electrically connected with multiple interfaces 50, for the data of master board to be converted into multiple interfaces 50Any of the information that can transmit of interface；Wireless communication module 70 is arranged in master board 40, is used for and exterior terminalRealize wireless telecommunications.
In the embodiment of the present invention, multiple interfaces are integrated in PCR instrument, can there is multiple and different species, the interface of signal, withSuitable for various exterior terminal interfaces, in this way, staff can be connected using various types of terminals with PCR instrument, without makingWith dedicated computer, you can it is monitored, also, by adding wireless communication module, can realize wireless monitor,As long as exterior terminal establishes wireless connection with PCR instrument, staff can anywhere realize the monitoring to PCR processes,Greatly improve convenience and the intelligence of PCR instrument.
Optionally, the wireless communication module of the embodiment of the present invention includes at least one of：WIFI module, bluetooth module,Zigbee module, mobile communication module.For wireless monitor closely, exterior terminal can with PCR instrument by bluetooth module,Zigbee module, WIFI module establish connection, realize wireless telecommunications and data monitoring, for remote monitoring, then can use and moveDynamic communication module is established long distance wireless with exterior terminal and is connected, and facilitates the reaction process and knot of staff's remote monitoring PCR instrumentFruit.
Further, mobile communication module includes：Gsm module, GPRS module, 3G module, 4G modules.
In the embodiment of the present invention, exterior terminal is one of the following：Router, desktop computer, mobile phone, tablet computer, notesThis computer.Wherein, when exterior terminal is router, it that is to say and the reaction process data and result of PCR instrument are uploaded to networkServer, staff can extract these data on network server.In the embodiment of the present invention, user can use individualThe terminals such as mobile phone, computer and PCR instrument, which are established, to be connected, so as to the monitoring to showing anywhere in fact.
In the embodiment of the present invention, thermocirculator is the main place of PCR reactions, including：Orifice plate, for placing sampleTest tube；Heating module is arranged below orifice plate, is connected with master board, for being heated or being cooled down；Hot cover, ifIt puts on sample tube, is contacted with sample tube mouth.
-- annealing -- is denatured to sample tube on orifice plate and extends three elementary processes, then by fluoroscopic examinationDevice realizes fluoroscopic examination, draws testing result.Further, intelligent PCR instrument further includes：Sub-control plate connects with hot coverIt connects, for controlling hot cover.Specifically, also sub-control plate both for controlling the temperature of hot cover, is additionally operable to controlHeat the movement of cover.
Optionally, as shown in Fig. 2, the intelligent PCR instrument of the embodiment of the present invention further includes：Master controller 401, is arranged on masterOn control panel 40, master controller 401 is divided by the interface in master board 40 and thermocirculator 20 and fluorescence detection device 30It is not electrically connected, for controlling the process of PCR；Submaster controller 402 is arranged in master board 40, with main controlDevice 401, wireless communication module 70 and multiple interfaces 50 are electrically connected, for handling the communication with exterior terminal.
In the embodiment of the present invention, the process of PCR is controlled by master controller 401, for example, temperature controls, is glimmeringLight detection control etc., by submaster controller 402 realize with the data communication before exterior terminal, can so reduce master controller401 load avoids the problem that breaking down to block since 401 load of master controller is excessive stopping.In addition, it is carried out using dual controllerControl, it is more efficient without using high performance single processor.
Optionally, the intelligent PCR instrument of the embodiment of the present invention further includes：Power detecting module, is electrically connected with master controller,For detecting the electrical energy parameter of power supply unit output；Shine detection module, is electrically connected with master controller, for detecting fluoroscopic examinationThe luminous parameters of device；Temperature sensor is electrically connected with master controller, for detecting the temperature of heating module；Hot lid temperature passesSensor is electrically connected with master controller, for detecting the temperature of hot cover.
In the present embodiment, the electrical energy parameter of power detecting module detection power supply unit output can reflect power supply unitWhether exception is occurred.Similarly, it is different that the luminous parameters that luminous detection module detects can reflect whether fluorescence detection device occursOften, specifically whether the luminescent system of reaction fluorescence detection device there is exception；The temperature parameter that temperature sensor detects canWhether reflection heating module there is exception；The heating for the hot cover that the temperature parameter of hot lid temperature sensor detection can reflectIt is whether abnormal.
According to embodiments of the present invention, the working condition of PCR instrument is detected by setting detection module or sensor, realizedSelf-test to PCR instrument so as to determine the component being abnormal in time, avoids utilizing the component sustained response being abnormalOr the problem of experiment reaction time caused by experiment extends, efficiency is low.
Further, the power detecting module of the embodiment of the present invention, luminous detection module, temperature sensor and Re Gai temperatureSensor is electrically connected respectively with submaster controller, and submaster controller is used for power detecting module, shine detection module, temperature sensorExterior terminal is sent to by wireless communication module and/or multiple interfaces with the testing result of hot lid temperature sensor.
In the embodiment of the present invention, the parameter that PCR instrument is carried out to self-test by submaster controller is sent to exterior terminal, so thatIt can be the working condition that may know that PCR instrument by exterior terminal to obtain user, in order to react in time, adjust work.
Obviously, the above embodiments are merely examples for clarifying the description, and is not intended to limit the embodiments.It is rightFor those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation orIt changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended out orAmong changing still in the protection domain of the application.