A kind of negative pressure device available for automatic instrument for extracting nucleic acidTechnical field
The present invention relates to a kind of negative pressure device, more particularly to a kind of negative pressure device available for automatic instrument for extracting nucleic acid.
Background technology
In modern molecular biology detection, the molecule diagnosis and detection technique based on nucleic acid are Sino-Japan in numerous areasBenefit shows vital effect.In Molecular Detection, 3 steps, i.e. nucleic acid extraction, amplification and detection are broadly divided into.It is firstIt is exactly fast and efficiently to extract and separate required nucleic acid from biological specimen to want problem.Nucleic acid extraction is detected downstream, gene pointThe starting point of type, is one of basic skills of molecular biology most critical, penetrates into biology, science of heredity, medicine etc. extensivelyEach life field, follow-up detection efficiency and accuracy will be directly affected by extracting separated Nucleic acid quality and its integrality, be risenThe key effect taken over from the past and set a new course for the future.
The operation such as precipitation and centrifugation needs a large amount of biological specimens, and tradition included in traditional nucleic acid extraction technologyTechnical step is relatively complicated, and time-consuming, and nucleic acid extraction efficiency is low, and operating personnel need to contact chemical reagent, and health is madeInto threat.The automation of nucleic acid extraction is needed for market, due to the development of molecular detection technology, applied to all trades and professions,Therefore height, such ability are had to the large-scale use for promoting molecular detection technology, the degree of automation of nucleic acid extractionAvoid the mistake caused by manual operation factor during experimental implementation.In order to avoid pollution of nucleic acid, Molecular Detection experimentRoom is traditionally arranged to be 4 rooms being relatively isolated, i.e. reagent preparation room, sample preparation room, PCR amplification area, detection of nucleic acids region,Each functional area is kept apart to avoid pollution.
The overall flow of detection of nucleic acids is basically identical, but each step has many methods, and each method has much not againSame reagent is available, and reagent producer is also more likely to carry out the production of corresponding instrument for the reagent of oneself productionSale, therefore also there is different self-reacting devices, therefore, how experimental implementation person is selected and is integrated these reagents and instrumentDevice is also a major challenge.
At present, domestic nucleic acid extraction mainly uses traditional craft preparation method.The method for automating comparative maturity is magneticPearl method nucleic acid extraction, Beads enrichment technology mainly use ozzle and external magnet, or the external magnet using plate tube.ButThe technique transfers of these Beads enrichments are solution rather than magnetic bead, and the loss amount of magnetic bead and sample is bigger, and cleaning performance is poor.It is negativePlaten press nucleic acid extraction also has some equipment, but is only limitted to small lot sample, and the degree of automation is low, and be easy to cause between sample and hand overFork pollution etc..A large capacity is developed for this can be applied to the device of negative pressure extraction nucleic acid and be particularly important, also to be full-automaticThe development of instrument for extracting nucleic acid provides theoretical foundation.
The content of the invention
The present invention provides a kind of negative pressure device available for automatic instrument for extracting nucleic acid, including：Base, motor, waste collectionDevice, nucleic acid collection device, lifter, moving guide rail, vacuum pump, buffering conical flask, three-way connection, valve.
The waste collecting device, nucleic acid collection device, lifter, moving guide rail are fixed on base.
The waste collecting device includes waste collection groove, fixing device.
The nucleic acid collection device includes nucleic acid absorption column tube frame, fixing device, nucleic acid collecting pipe pipe support.
Further, nucleic acid absorption column tube frame is fixed by fixed plate and moving guide rail, nucleic acid absorption column tube frame and fixationDevice can be driven by motor and submit change place movement in waste collecting device, nucleic acid collection device, driven and lifted by motorDevice lifts fixing device and moves along guide rail movement, the motor of the driving nucleic acid absorption column tube frame movement for stepper motor andServomotor.
After the vacuum pump, buffering conical flask, three-way connection composition combination and the fixing device with being fixed on base connectsIt is logical.
Further, it is described buffering conical flask be connected with vacuum pump, when vacuumizing from cushioning effect, the three-way connectionIt is connected with buffering conical flask, and is connected with the fixing device of waste collecting device and nucleic acid collection device take out very respectivelyIt is empty.
Beneficial effect is caused by the present invention：
In the present invention, a kind of negative pressure device available for automatic instrument for extracting nucleic acid can compatible different manufacturersNucleic acid extracting reagent come complete sample nucleic acid extraction work device, while also can further based on negative pressure device intoThe research and development of row nucleic acid full-automation extraction platform, the device operating cost is low, easy to operate, will more and more be applied.The device has the following advantages：
1. rapid extraction：Extraction specimen amount is big, and stability is good, and can effectively avoid traditional negative pressure extraction device, there are pressureEasily leakage, it is unstable, handle the defects of work efficiency is low, and capacity is small.
2. accurate control, high DNA purity and efficiency, make nucleic acid extraction reach high-purity, high extracting efficiency.
3. result is stablized：Difference caused by manual operation and mistake are avoided, it is reproducible；Purification side can be optimized according to reagentCase, realizes the extraction efficiency of higher, and the nucleic acid purity of extraction is high.
Brief description of the drawings
Fig. 1 is a kind of overall structure diagram of negative pressure device available for automatic instrument for extracting nucleic acid of the invention；
Fig. 2 is a kind of negative pressure device fractionation structural representation available for automatic instrument for extracting nucleic acid of the invention；
Fig. 3 be a kind of negative pressure device available for automatic instrument for extracting nucleic acid of the invention vacuum pump and waste collecting device,The structure diagram that nucleic acid collection device is connected.
Embodiment
With reference to specific embodiment, the present invention will be described in detail.Specific embodiment is only without prejudice to this hairThe lower limited list of bright spirit, it is not excluded that those of ordinary skill in the art the prior art and the present invention with reference to and generationOther specific embodiments.
In order to reach the purpose of the present invention, one embodiment of the present invention is：One kind can be used for automatic instrument for extracting nucleic acidNegative pressure device, including：Base 15, motor 1, motor 3, waste collecting device 4, nucleic acid collection device 2, lifter 6, movement are ledRail 5, vacuum pump 17, buffering conical flask 18, three-way connection 20, valve 19 and valve 21.
The waste collecting device 4, nucleic acid collection device 2, lifter 6, moving guide rail 5 are fixed on base 15.
The waste collecting device 4 includes waste collection groove 11, fixing device 13.
The nucleic acid collection device 2 includes nucleic acid absorption column tube frame 7, fixing device 8, nucleic acid collecting pipe pipe support 12, solidDetermine device 14.
Further, nucleic acid absorption column tube frame 7 is fixed by fixed plate 9 and fixed plate 10 with moving guide rail 5, nucleic acid absorptionColumn tube frame 7 and fixing device 8 can be driven by motor 1 and motor 3 to be exchanged on waste collecting device 4, nucleic acid collection device 2Position is moved, and is driven lifter 6 to lift fixing device 8 by motor 1 and is moved along guide rail 5 and moves, the driving nucleic acid absorptionThe motor 1 that column tube frame 7 moves is stepper motor, and motor 3 is servomotor.
After the vacuum pump 17, buffering conical flask 18, the composition combination of three-way connection 20 and with being fixed on consolidating on base 15Determine device 13 to connect with fixing device 14.
Further, it is described buffering conical flask 18 be connected with vacuum pump 17, when vacuumizing from cushioning effect, the threewayConnector 20 is connected with buffering conical flask 18, and respectively with the fixing device 13 of waste collecting device 4 and nucleic acid collection device 2 and consolidatingDetermine device 14 to be connected and vacuumized.
It is 48 hole pipe supports that the nucleic acid, which collects grillage 12, is arranged with the combination of 6X8, preferable collecting pipe is 0.6mlCentrifuge tube；Nucleic acid absorption column tube frame 7 is 48 holes, is arranged with the combination of 6X8, and nucleic acid absorption column tube frame 7 can insert nucleic acid and carryColumn is taken, nucleic acid collects grillage 12, adsorption column pipe support 7, the combination of waste collection groove 11 and fixation by fixing device 13 and fixed dressPut 14 completions.Fixing device has two, is connected respectively at valve 19 with valve 21.
Further, it is connected respectively with waste collecting device 4 and nucleic acid collection device 2 using three-way connection 20 and is taken outVacuum, waste collecting device 4 and nucleic acid collection device 2 can be carried out taking out very respectively by the on and off of valve 19 and valve 21Sky forms negative pressure, it is possible to prevente effectively from carrying out the sample nucleic acid cross contamination situation caused by negative pressure extraction using same pipeline.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow ordinary skill peopleMember can understand present disclosure and be carried out, it is not intended to limit the scope of the present invention, it is all according to the present inventionThe equivalent change or modification that Spirit Essence is made, should all cover within the scope of the present invention.