A kind of microencapsulation seeweed polyphenol extract and preparation method thereofTechnical field
The present invention relates to the technical field of seeweed polyphenol, and in particular to the preparation method of seeweed polyphenol extract.
Background technology
Seeweed polyphenol (algae polyphenols, AP) is the polyphenol compound general name extracted from seaweed, isHas the secondary metabolite of seaweed, and seaweed protects itself from the active principle of adventive interference.Seeweed polyphenol conductThe main source of sea-plant polyphenol, the land plant polyphenol such as its structure and tea polyphenols, apple polyphenol, Punica granatum L. polyphenol exist obviousDifference, seeweed polyphenol basic structural unit are phloroglucins, and simply phloroglucin connection mode is different with quantity.Wherein in brown algaPolyphenol content is compared with horn of plenty, and brown alga has the characteristics that distribution is wide, species is more, yield is high, so brown alga is that extraction separation is moreThe excellent algae of phenol.
Seeweed polyphenol have it is antitumor, anti-oxidant, numerous physiological activity such as antibacterial, antiviral, not only there is very strong pharmacologyActivity, and brown algae polyphenols belong to less toxic chemical substance, edible safety.In October, 2017 major website orientation European Union assessment kelpNews of the brown algae polyphenols extract as the security of new resource food, it is food supplement to claim this new resource food work done in the manner of a certain authorFor the normal adults of more than 12 years old, with people's multi-functional understanding many to polyphenol and the further depth of plant polyphenol researchEnter, can predict that seeweed polyphenol future will have wider array of market development prospect.
The research of current seeweed polyphenol is concentrated mainly on the preparation of the extracting method and high-purity polyphenol product of polyphenolIn improvement, such as Chinese patent CN201510529461.2；CN201710070691.6；CN2012105226792.7；CN201610611275.8；CN201510648035.0, but these patents be only absorbed in the extraction process of polyphenol, extraction efficiency andProduct purity, and the unstability of polyphenol property is ignored, polyphenol is easily aoxidized, particularly easily rotten under light illumination, at the same timePolyphenol contains multiple hydroxyls, easily forms hydrogen bond with water so that and, there is the characteristics of not easy to maintain in dried sample easy to absorb moisture,And some techniques are complex or equipment requirement is higher, be not suitable for large-scale production.Meanwhile seeweed polyphenol is as exclusiveSea-plant property polyphenol, its extract influence eating mouth feel, always there are the fishy smell of a degree of seaweed and the bitter taste of polyphenolIt, due to presence of the seeweed polyphenol there are content the problems such as relatively low, unstable oxidizable, mouthfeel is bad, limits seeweed polyphenolIndustrialized development, therefore there is an urgent need to develop the product that a kind of polyphenol content is high, property is stable, is easy to preserve and eats.
Microencapsulation technology is a kind of technology for using different wall materials to be embedded active material, and wall material has natural polymerSub- material and synthesis high molecular material, and apply the features such as natural macromolecular material is because of stabilization, nontoxic, good film-forming property it is more,More commonly used natural macromolecular material has glue, polysaccharide, protein etc.., can by selecting different wall materials to carry out embedding treatmentPlaying improves the stability of active material, changes its physical property, control release, isolation active ingredient, shielding taste and smellEtc. a series of effects..
The content of the invention
It is an object of the invention to solve bad current seeweed polyphenol stability, the easy moisture absorption, mobility is bad and mouthfeel is owedGood problem, there is provided a kind of microencapsulation seeweed polyphenol extract, extraction and separation process are combined with microencapsulation technology of preparing, are made micro-Encapsulated seeweed polyphenol extract stability is good, being not easy the moisture absorption, mobility, good, mouthfeel is easy to receive.In addition the present invention also provides oneThe preparation method of kind microencapsulation seeweed polyphenol extract, technique is simple, easily operated, and it is excellent that properties of product are made.
For achieving the above object, the present invention uses following technical scheme：
A kind of microencapsulation seeweed polyphenol extract, core are seeweed polyphenol extract, and wall material is that mass ratio is 1:3-5 Arabic gumsAnd polysaccharide, wherein polysaccharide is one or more kinds of equal proportion mixtures in chitosan, maltodextrin, cyclodextrin.
The preparation method of microencapsulation seeweed polyphenol extract of the present invention, comprises the following steps：
The extraction of step (1), seeweed polyphenol：New fresh seaweed is sieved after drying and crushing, is added 50-90% ethanol and is extracted,Wherein solid-liquid ratio (m/v) is 1:15-25, Extracting temperature are 30-50 DEG C, when extraction time is 4-6 small；
The separation of step (2), seeweed polyphenol：By step（One）The extracting solution of acquisition is concentrated under reduced pressure at 40-60 DEG C recycles ethanol,The macroreticular resin handled well on concentrate, is first eluted with pure water after the completion of absorption and removes polysaccharide, inorganic ions impurity, then use bodyProduct concentration 50-90% ethanol elutions obtain polyphenol eluent, elution speed 2-4BV/h, and eluent dosage is 3-5BV, and polyphenol elutesLiquid is recovered under reduced pressure ethanol and obtains seeweed polyphenol extracting solution, and polyphenol content is more than 30% in this extracting solution；
The preparation of step (3), seeweed polyphenol microcapsules：By step（Two）In seeweed polyphenol extracting solution as core, with mePrimary glue and polysaccharide are wall material, and first wall material is dissolved in pure water, are made into the solution that concentration is 1-5%, then add seeweed polyphenol and carryTake in liquid and stir evenly, it is 8%-12% to be concentrated under reduced pressure into solid content, and spray drying obtains microencapsulation seeweed polyphenol extract.
Wherein, the step（One）The high brown alga of middle seaweed, preferably polyphenol content, including kelp, kelp, sargassum fusifome, skirtBand dish and its sporophyll etc..
The step（Two）Middle macroreticular resin is AB-8 or NKA-9 type macroreticular resins
The step（Three）Middle wall material and core mass ratio 1-3:1.
Using above-mentioned technique it is a technical advantage that the following aspects：
（1）By taking a variety of methods, the higher seeweed polyphenol extract of content is obtained（Polyphenol content is more than 30%）, such as chooseThe high brown alga of polyphenol content is extracted for raw material, using the ethanol of various concentrations, using macroreticular resin further clean and pureChange.
（2）By the extraction process of seeweed polyphenol with microcapsule preparation process rationally, be effectively combined, realize onceProperty feed intake and can obtain the product of microencapsulation, technological process is simple, rationally, on the premise of equipment is not increased, shortens lifeThe flow of production. art and time, and it is easy to the production of large-scale.
（3）Pass through the technology of preparing of microcapsules so that stability, physical behavior and the mouthfeel of seeweed polyphenol product obtainImprove, the fishy smell of seaweed and the bitter taste of polyphenol decrease in the product produced, while product has that stability is good, flowingProperty it is good and the characteristics of be not easy the moisture absorption, be conducive to holding and use and the increase bioavilability of product, so that it is more to expand seaweedApplication of the phenol in each field.
Embodiment
The preparation process of microencapsulation seeweed polyphenol extract of the present invention is made by specific examples below mode more detailedExplanation：
Embodiment one：
Microencapsulation seeweed polyphenol extract, prepares according to following step：
The extraction of step (1), seeweed polyphenol：By dry Undaria pinnatifida Suringar 500g grinding and sievings, it is 50% to add concentrationEthanol is extracted, and solid-liquid ratio (m/v) is 1:20,50 DEG C of Extracting temperature, when extraction time is 6 small；
The separation of step (2), seeweed polyphenol：By step（One）The extracting solution of acquisition is concentrated under reduced pressure at 60 DEG C recycles ethanol to oneDetermine volume, the AB-8 macroreticular resins handled well on concentrate, are first eluted with pure water after completion of the sample, remove polysaccharide, inorganic ionsDeng impurity, polyphenol eluent then is obtained with 90% ethanol elution, elution speed is 3 BV/h, and eluent dosage is 3BV, and polyphenol is washedDe- liquid is recovered under reduced pressure ethanol and obtains seeweed polyphenol extracting solution, and polyphenol content is 51.2% in this extracting solution；
The preparation of step (3), seeweed polyphenol microcapsules：By step（Two）In seeweed polyphenol extracting solution as core, with mePrimary glue, chitosan are wall material（Both mass ratioes are 1：1）, first by wall material be made into certain volume pure water concentration be 3% it is moltenLiquid, by wall material and core 3：1 ratio is added in seeweed polyphenol extracting solution and stirred evenly, and being concentrated under reduced pressure into solid content isIt is spray-dried after 10% certain volume, it is final to obtain microencapsulation seeweed polyphenol extract.
Embodiment two：
Microencapsulation seeweed polyphenol extract, prepares according to following step：
The extraction of step (1), seeweed polyphenol：By dry skirt kelp 500g grinding and sievings, add concentration for 90% ethanol intoRow extraction, solid-liquid ratio 1:15,30 DEG C of Extracting temperature, when extraction time is 4 small；
The separation of step (2), seeweed polyphenol：By step（One）The extracting solution of acquisition is concentrated under reduced pressure at 40 DEG C recycles ethanol to oneDetermine volume, the NKA-9 macroreticular resins handled well on concentrate, are first eluted after loading is complete with pure water, remove polysaccharide, inorganic ions etc.Impurity, then obtains polyphenol eluent, elution speed 2BV/h with 70% ethanol elution, and eluent dosage is 4BV, polyphenol eluentEthanol is recovered under reduced pressure and obtains seeweed polyphenol extracting solution, polyphenol content is 37.8% in this extracting solution；
The preparation of step (3), seeweed polyphenol microcapsules：By step（Two）In seeweed polyphenol extracting solution as core, with mePrimary glue, porous-starch, maltodextrin are wall material（Both mass ratioes are 1：2：2）, first it is made into through wall material with certain volume pure water denseThe solution for 5% is spent, by wall material and core 1：1 ratio is added in seeweed polyphenol extracting solution and stirred evenly, and is concentrated under reduced pressure into solidThing content is final to obtain microencapsulation seeweed polyphenol extract to be spray-dried after 12% certain volume.
Measure product refrigerate and room temperature preservation under the conditions of, the change of polyphenol content, the results show that with polyphenol extract phaseComparing, the seeweed polyphenol extract stability of microencapsulation substantially increases, and is increased to minimum 3 months from the storage cycle of one month,The physical behavior of product and mouthfeel are improved at the same time, and the fishy smell of seaweed and the bitter taste of polyphenol decrease, and productPowder is uniform, and good fluidity, is not easy adhesion, is not easy the moisture absorption, is conducive to the holding and use of product.
The above is merely illustrative of the technical solution of the present invention, every technology based on claims of the present invention contentScheme, belongs to protection scope of the present invention.