A kind of biology sensor and its detection methodTechnical field
The present invention relates to bioengineering field, relates in particular to a kind of biology sensor and its detection method.
Background technology
When carrying out tissue fluid detection to human body or animal body, biology sensor is often used.Biology sensor oneAs be concentration for detecting composition in tissue fluid, the working electrode in biology sensor is different according to its size of current, is adapted toThere is also difference, such as same type of tissue fluid components utilising for Different Individual various concentrations scope in the accurate region of detectionWorking electrode detected, easily occur detecting the problem of not accurate.
For example, the tissue fluid concentration for being mainly characterized by slowly persistently rising of diabetes, in order to detect human blood glucose concentration,In the market has a variety of blood sugar test instruments.The Chinese patent of Application No. 201180063434.0 discloses a kind of detectable concentrationMethod, the system for detecting blood sugar break epidermis by fine acupuncture, and direct monitoring tissue liquid blood sugar concentration, the degree of accuracy is higher, butIt is to need to puncture human body skin, belongs to minimal invasive techniques, and consumptive material is extremely expensive.
Also a kind of bioelectrode by reverses iontophoresis method, reverses iontophoresis method in epidermis by applying electricity, the Na ions in tissue fluid are to movable cathode, and the Cl ions Ghandler motion that faces south moves, and skin is negatively charged under normal circumstances, Na ionsAs the ion stream of main carriers, neutral glucose molecule is carried to skin surface, realizes the purpose of noninvasive extraction.However,Blood sugar test product based on reverses iontophoresis method, minimal irritation skin when extracting interstitial fluid be present, working electrode measurement bloodThe problem of sugared concentration range is narrow, detection is not accurate.
The content of the invention
In order to solve drawbacks described above, a kind of present invention blood sugar test scope of offer is big, can oneself detection data be carried outCalibration identification, detects more accurately biology sensor.
In order to reach this effect, the technical solution adopted by the present invention is as follows：A kind of biology sensor, including substrate,Electrod-array is printed with the one side of the substrate, the electrod-array includes being used to extract taking out for tissue liquid to be detectedPower taking pole, the working electrode for detecting tissue fluid composition and for calibration electrode operating voltage reference electrode andMultiple is arranged to electrode, the working electrode for forming loop with working electrode, the detection zone of multiple working electrodesArea it is different.
In general, substrate includes working region and wire area, and the electric wire on wire area is easily made to working regionInto interference, so covered with insulating barrier typically on wire area, insulating barrier also acts as the effect for preventing connecting line short circuit.
The size of multiple working electrodes is different to cause them to have different operating currents, so multiple working electrodesCan measures various concentrations scope tissue fluid parameter.In general, working electrode area is bigger, its measurable tissue fluid parameterConcentration range it is smaller.
Preferably, multi-group electrode array is provided with over the substrate, and multi-group electrode array detects human body not same district respectivelyThe biological parameter in domain.Multi-group electrode array is set on one substrate, and when mounted, multi-group electrode array is covered in human body skinDifferent regions.
Preferably, the multi-group electrode array takes turns to operate.When electrod-array works, extract electrode and to extract at the moment and coverThe tissue liquid of cover area, in order to avoid extraction of the long-time to a region causes wound, multi-group electrode array to human bodyTypically staggeredly work in turn.
Preferably, it is described extract electrode set electrod-array outermost and around inner side electrod-array by inner sideIncluding electrod-array " parcel ".Extract electrode and be arranged on the outermost of electrod-array, during work, extract the group that electrode extracts epidermisLiquid is knitted, then tissue fluid is diffused into working electrode area to centre, is easy to working electrode absorption detecting tissue fluid composition.
Preferably, the extraction electrode is integrally in " falling U " types, the working electrode, reference electrode and electrode is respectively provided with" falling in the extraction electrode of U " types, described " opening that the U " types that fall extract electrode is used for picking out lead." U " types that fall extract electrodeSetting cause internal work electrode distance extract electrode position it is all relatively near, aspirates tissue liquid is more uniformly accurate, is easy to carryHigh detection precision.
Preferably, the electrod-array is two groups, and two arrays of electrodes array is symmetrical arranged.
Preferably, over the substrate working electrode, reference electrode, the region to electrode and tissue fluid absorption electrode are coveredLid layer of gel disk, detection enzyme is provided with the gel disk.Gel disk includes PEO, polyvinyl alcohol (PVA), shell and gatheredSugar or agarose；Detection enzyme component also includes bovine serum albumin (BSA) and/or buffer solution (PBS) and/or glucose in gel diskOxidizing ferment (GOD) and/or preservative and/or NMF and/or penetrating agent and/or complexing agent and/or anti-interference agent.Detect enzymeSpecific composition can detect human-body biological parameter adjustment according to specific needs.
Preferably, the wire picked out over the substrate is connected in the control unit of substrate back, single in the controlThe bottom of member is additionally provided with the fixing device for described control unit, substrate and gel disk to be fixed, and connects in the fixing deviceIt is connected to the connect band for biology sensor to be fixed on to human body skin top layer.
In order to further improve biology sensor in detection to the precision of multiple working electrode data calibrations, the present inventionA kind of detection method of biology sensor is additionally provided, is comprised the following steps：
A, biology sensor is arranged on human body skin top layer；
B, electrod-array is started working, and the extraction electrode on electrod-array extracts tissue liquid；
C, the tissue fluid extracted is diffused near working electrode, and multiple working electrodes read detection data；
D, the tissue fluid concentration data of the working electrode detection of different area size is transferred to control unit in electrod-array,Itself and default measurement concentration ranges are carried out comparison calibration by controller, obtain final detection numerical value.
Preferably, in the step b, electrod-array is multigroup, and first group of electrod-array is first started working, and treats a timingBetween after another set electrod-array start working, multi-group electrode array works in human body skin factor of different region staggered cycles.
Preferably, the measurement concentration calibration method in the step c is as follows：
Human blood glucose concentration is divided into multiple sections, respectively C1, C2 ... CN in advance, it is single to store the data to controlIn the holder of member；
The work area of multiple working electrodes on each electrod-array is corresponded into the more of blood sugar concentration according to measurement rangeIndividual section is set, respectively S1, S2 ... SN；Store the data in the holder of control unit；
The working electrode of S1 areas is defined as the standard working electrode in C1 tissue fluid concentration ranges, the work of S2 areasElectrode is positioned as the standard working electrode ... in C2 tissue fluid concentration ranges, and the working electrode of SN areas is defined as CN tissue fluidStandard working electrode in concentration range, is stored the data in the holder of control unit；
Data B1, B2, B3 ... BN that several working electrodes measure are fed back into control unit, control unit by its withPreset data in holder is contrasted, and obtains final detection numerical value.
Preferably, it is characterised in that its control methods is：By different operating measurement data according to default concentration ranges pointCloth, it is most in that default concentration ranges to see that measurement data falls：
If measurement data fall into most concentration ranges for one when, corresponding to the concentration ranges standard working electrode measureConcentration be defined as final measured value；
If measurement data fall into most concentration ranges for it is multiple when, multiple standards corresponding to the plurality of concentration rangesThe flat quasi- value of working electrode measurement data is final measured value.
The beneficial effects of the invention are as follows：Compared with existing biology sensor, the present invention is provided with same electrod-arrayMultiple working electrodes, it can thus meet the tissue fluid detection of different tissues liquid concentration difference human body, tissue fluid detection rangeGreatly；In addition, the data detected simultaneously also directed to multiple working electrodes in the present invention have carried out identification calibration, the identification calibration sideMethod make it that the data of detection are more accurate.
Brief description of the drawings
Fig. 1 is electrode array configurations schematic diagram；
Fig. 2 is substrate face structural representation；
Fig. 3 is substrate back structural representation；
Fig. 4 is biological sensor fixing structure schematic diagram.
Fig. 5 is biology sensor testing process schematic diagram.
Wherein 1 is substrate, and 2 be electrod-array, and 3 be working electrode, and 4 is extract electrode, and 5 be reference electrode | to electrode, 6It is wire connecting portion for connecting line, 61,7 be working region, and 8 be wire area, and 9 be insulating barrier, and 10 be gel disk, and 11 is single for controlMember, 12 be fixing device, and 13 be fixing band.
Embodiment
For the ease of the understanding of the present invention, it is described below in conjunction with the accompanying drawings：
As Figure 1-4, invention provides a kind of biology sensor, including substrate 1, print of the substrate on one sideThere is electrod-array 2, electrod-array 2 includes being used to extract the extraction electrode 4 of tissue liquid to be detected, for detecting tissue fluidThe working electrode 3 of composition and for calibration electrode operating voltage reference electrode 5 and for being formed with working electrodeLoop is arranged to multiple to electrode 5, working electrode 3, and the area of the detection zone of multiple working electrodes is different.
The size of working electrode 3 is different, and there is also difference, the working electrode of different operating electric current to survey for its operating currentDuring amount, the scope of the physiological parameter such as its tissue fluid detected is also different.Because Different Individual or same individual are when differentBetween in the cycle its tissue fluid concentration have differences, using the working electrode of different area size, its accurate testing degree is different, noHad differences with working electrode for different range tissue fluid measurement of concetration effect.So in the present invention in same electrode arrayMultiple working electrodes are used on row, as preferred embodiment, 3 working electrodes are employed in the present invention, such as figure working electrode31st, working electrode 32, working electrode 33.Multiple working electrodes work simultaneously, can for the tissue fluid scope of various concentrationsAccurately measure..
On substrate 1, electrod-array 2 is connected to 1 feather edge part of lining by connecting line 6 and forms wire connecting portion 61, and substrate includesWorking region 7 and wire area 8, due to having electric current on wire area connecting line by the way that current signal can be to the inspection of bioelectrodeSurvey interferes, in order to avoid this problem, typically covered with insulating barrier on wire area.According to specifically used scene, electricityThe connecting line of pole array can be connected on substrate with electrod-array in the edge of the same face, can also be connected to the edge of reverse sidePlace.In order to avoid connecting line interferes to electrod-array, insulating barrier 9 is covered on the non-active area that connecting line passes through.
The wire picked out on substrate 1 is connected in the control unit 11 of substrate back, is also set in the bottom of control unitThe fixing device 12 for described control unit 11, substrate 1 and gel disk 10 to be fixed is equipped with, use is connected with the fixing deviceIn the connect band 13 that biology sensor is fixed on to human body skin top layer.
In the present invention, multi-group electrode array is provided with the same substrate, and multi-group electrode array is covered in human body skinUpper different zones detection, the work measurement that multi-group electrode array can be independent.At work, each group of electrod-array coversHuman body skin area is different, because biology sensor is longer in the skin surface working time, if extracting electrode long-time oneCause to carry out tissue fluid extracting to region that certain wound can be caused to human body, in order to avoid this problem, in the present inventionMultigroup working electrode staggeredly works, and when an electrod-array works, other electrod-arrays are stopped, and avoid in electrod-arrayExtraction electrode works long hours causes wound to human body.
As a comparison preferred embodiment, electrod-array is two groups, and two arrays of electrodes array is symmetrical arranged.Certainly, it is electricThe particular number of pole array, and the method for distribution can adjust according to concrete application scene.
In general, extract electrode 4 be arranged on electrod-array outermost and around inner side electrod-array by inner sideElectrod-array " parcel " including.Extract electrode 4 and be arranged on the outermost of electrod-array, during work, extract electrode and extract epidermisTissue fluid, then tissue fluid be diffused into working electrode area to centre, be easy to working electrode absorption detecting tissue fluid composition.
As a kind of embodiment, electrode is extracted integrally in " falling U " types, working electrode, reference electrode and electrode is respectively provided with" falling in the extraction electrode of U " types, described " opening that the U " types that fall extract electrode is used for picking out lead." U " types that fall extract electrodeSetting cause internal work electrode distance extract electrode position it is all relatively near, aspirates tissue liquid is more uniformly accurate, is easy to carryHigh detection precision.Certainly " circle " type with opening can also be arranged to by extracting electrode, and its concrete shape can be according to realityApplication scenarios and application demand adjustment.
Working electrode, reference electrode on substrate, one layer of region overlay for drawing to electrode and tissue fluid electrode are solidifyingLacquer disk(-sc) 10, detection enzyme is provided with gel disk.Detection enzyme reacts with tissue liquid, dense to reach detection tissue fluid compositionThe purpose of degree.
Gel disk includes PEO, polyvinyl alcohol (PVA), chitosan or agarose；Detection enzyme component in gel diskAlso include bovine serum albumin (BSA) and/or buffer solution (PBS) and/or glucose oxidase (GOD) and/or preservative and/or guarantorHumectant and/or penetrating agent and/or complexing agent and/or anti-interference agent.The specific composition of detection enzyme can detect people according to specific needsBody biological parameter adjusts.
As shown in figure 5, in order to further improve biology sensor in detection to the essence of multiple working electrode data calibrationsAccuracy, present invention also offers a kind of detection method of biology sensor, comprise the following steps：
A. sensor is arranged on human body skin top layer；
B. electrode works, and the extraction electrode on electrod-array extracts tissue liquid；
Number of poles group is multigroup, as preferred embodiment, it can be provided two groups, during work, first group of electrod-array is first openedBeginning work, another set electrod-array is started working after certain time, and two arrays of electrodes array is handed in human body skin factor of different regionWrong circulation work, it thus can staggeredly extract different human body skin area.Tissue fluid, reduce the wound to human body.
C. the tissue fluid extracted is diffused near working electrode, and multiple working electrodes read detection data；
D. the tissue fluid concentration data of the working electrode detection of different area size is transferred to control unit in electrod-array,Itself and default measurement concentration ranges are carried out comparison calibration by controller, obtain final detection numerical value.
Tissue fluid concentration is divided into multiple sections, respectively C1, C2 ... CN in advance, stores the data to control unitIn holder；
Multiple sections that the work area of multiple working electrodes in array corresponds to tissue fluid concentration according to measurement range are setPut, respectively S1, S2 ... SN；Store the data in the holder of control unit；
The working electrode of S1 areas is defined as the standard working electrode in C1 tissue fluid concentration ranges, the work of S2 areasElectrode is positioned as the standard working electrode ... in C2 tissue fluid concentration ranges, and the working electrode of SN areas is defined as CN tissue fluidStandard working electrode in concentration range, is stored the data in the holder of control unit；
Data B1, B2, B3 ... BN that several working electrodes measure are fed back into control unit, control unit by its withPreset data in holder is contrasted, and obtains final detection numerical value.
In general, the control methods is：Different operating measurement data is distributed according to default concentration ranges, sees measurementData fall most in that default concentration ranges：
If measurement data fall into most concentration ranges for one when, corresponding to the concentration ranges standard working electrode measureConcentration be defined as final measured value；
If measurement data fall into most concentration ranges for it is multiple when, multiple standards corresponding to the plurality of concentration rangesThe flat quasi- value of working electrode measurement data is final measured value.
Above example is only present pre-ferred embodiments, not limiting the present invention, it is noted that allAny modification, equivalent substitution and improvements made within the spirit and principles in the present invention etc. protection scope of the present invention itIt is interior.