技术领域technical field
本发明属于生物检测技术领域。The invention belongs to the technical field of biological detection.
背景技术Background technique
蛋白质的定量分析是生物化学和其它生命学科最常涉及的分析内容，是临床上诊断疾病及检查康复情况的重要指标，也是许多生物制品，药物、食品质量检测的重要指标。在生物实验中，对样品中的蛋白质进行准确可靠的定量分析，是经常进行的一项非常重要的工作。蛋白质是一种十分重要的生物大分子：它的种类很多，结构不均一，分子量又相差很大，功能各异，这样就给建立一个理想而又通用的蛋白质定量分析的方法代来了许多具体的困难。目前测定蛋白质含量的方法有很多种，下面列出根据蛋白质不同性质建立的一些蛋白质测定方法：物理性质：紫外分光光度法。化学性质：凯氏定氮法、双缩脲法、Lowry法，BCA法，胶体金法。染色性质：考马氏亮蓝染色法、银染法。其他性质：荧光法蛋白质测定的方法很多，但每种方法都有其特点和局限性，因而需要在了解各种方法的基础上根据不同情况选用恰当的方法，以满足不同的要求。例如凯氏定氮法结果最精确，但操作复杂，用于大批量样品的测试则不太合格；双缩脲法操作简单，线性关系好，但灵敏度差，样品需要量大，测量范围窄，因此在科研上的应用受到限制；而酚试剂法弥补了它的缺点，因而在科研中被广泛采用，但是它的干扰因素多；考马氏亮兰染色法因其简便开始重新受到关注；BCA法又以其试剂稳定，抗干扰能力较强，结果稳定，灵敏度高而受到欢迎，但检测成本较高。Quantitative analysis of protein is the most commonly involved analysis content in biochemistry and other life sciences. It is an important indicator for clinical diagnosis of diseases and inspection of recovery, and is also an important indicator for quality testing of many biological products, drugs, and food. In biological experiments, accurate and reliable quantitative analysis of proteins in samples is a very important work that is often carried out. Protein is a very important biomacromolecule: it has many types, uneven structure, great difference in molecular weight, and different functions, so it brings a lot of specificity to the establishment of an ideal and general protein quantitative analysis method. Difficulties. There are many methods for measuring protein content. Some protein determination methods established according to different properties of proteins are listed below: Physical properties: UV spectrophotometry. Chemical properties: Kjeldahl method, biuret method, Lowry method, BCA method, colloidal gold method. Staining properties: Coomassie brilliant blue staining, silver staining. Other properties: There are many methods for fluorescent protein determination, but each method has its characteristics and limitations. Therefore, it is necessary to choose the appropriate method according to different situations on the basis of understanding various methods to meet different requirements. For example, the Kjeldahl method is the most accurate, but the operation is complicated, and it is not qualified for the test of a large number of samples; the biuret method is simple to operate and has a good linear relationship, but the sensitivity is poor, the sample needs a large amount, and the measurement range is narrow. Therefore, the application in scientific research is limited; and the phenol reagent method makes up for its shortcomings, so it is widely used in scientific research, but it has many interference factors; Coomassie brilliant blue staining method has begun to receive attention again because of its simplicity; BCA The method is popular for its stable reagents, strong anti-interference ability, stable results and high sensitivity, but the detection cost is relatively high.
发明内容Contents of the invention
本发明的目的是：提供一种荧光微量蛋白的检测装置及检测方法，它采用荧光染料与特异性的靶分子结合，大大提升样品检测的灵敏度和准确性。The purpose of the present invention is to provide a detection device and detection method for fluorescent trace proteins, which use fluorescent dyes to bind specific target molecules, thereby greatly improving the sensitivity and accuracy of sample detection.
本发明的技术方案是：本装置包括：整机壳体、样品槽盖、触摸显示屏、硬件电路板、荧光检测光路。所述荧光检测光路包括：样品槽、激发光源、激发光探测单元、荧光探测单元、带通滤光片、光传输通道、屏蔽罩。The technical solution of the present invention is: the device includes: a complete machine housing, a sample tank cover, a touch display screen, a hardware circuit board, and a fluorescence detection optical path. The optical path for fluorescence detection includes: a sample tank, an excitation light source, an excitation light detection unit, a fluorescence detection unit, a bandpass filter, an optical transmission channel, and a shielding cover.
本发明的技术方案是：Technical scheme of the present invention is:
本装置包括激发光源A、激发光源B、激发光探测单元A、激发光探测单元B、荧光探测单元A、荧光探测单元B、滤光片A、滤光片B、滤光片C、滤光片D、样品槽、光传输通道、信号检测单元、主控单元、激发光控制单元、液晶显示单元、电路板、壳体、样品槽盖、屏蔽罩。The device includes excitation light source A, excitation light source B, excitation light detection unit A, excitation light detection unit B, fluorescence detection unit A, fluorescence detection unit B, filter A, filter B, filter C, filter Sheet D, a sample slot, an optical transmission channel, a signal detection unit, a main control unit, an excitation light control unit, a liquid crystal display unit, a circuit board, a casing, a sample slot cover, and a shielding cover.
激发光源A(1)位于样品槽(11)左侧，激发光源B(2)位于样品槽(11)右侧，分别焊接在电路板(17)上，嵌于光传输通道(12)内，用于激发样品中的荧光物质。The excitation light source A (1) is located on the left side of the sample slot (11), and the excitation light source B (2) is located on the right side of the sample slot (11), respectively welded on the circuit board (17) and embedded in the light transmission channel (12), Used to excite fluorescent substances in samples.
激发光探测单元A(3)位于激发光源A(1)与样品槽(11)之间，激发光探测单元B(4)位于激发光源B(2)与样品槽(11)之间，激发光探测单元B(4)与样品槽(11)高度均低于激发光源B(2)，激发光探测单元B(4)与样品槽(11)焊接在电路板(17)上，嵌于光传输通道(12)内，保证激发光可以照射到样品槽(11)内样品，用于对激发光的状态进行实时监测，确认激发光是否正常工作、光强度是否稳定。The excitation light detection unit A (3) is located between the excitation light source A (1) and the sample chamber (11), the excitation light detection unit B (4) is located between the excitation light source B (2) and the sample chamber (11), and the excitation light The detection unit B (4) and the sample groove (11) are both lower than the excitation light source B (2), and the excitation light detection unit B (4) and the sample groove (11) are welded on the circuit board (17) and embedded in the light transmission In the channel (12), it is ensured that the excitation light can be irradiated to the sample in the sample chamber (11), which is used to monitor the state of the excitation light in real time, and confirm whether the excitation light is working normally and whether the light intensity is stable.
荧光探测单元A(5)、荧光探测单元B(6)分别垂直位于样品槽(11)与激发光光路的两侧，焊接于电路板(17)上，嵌于光传输通道(12)内。接收并检测由样本槽(11)内待测样本发出的荧光信号强度，均采用高精度、高灵敏度的光电二极管，可提高荧光检测精度，测量范围为300-1,000nm。这些荧光染料只有与这些靶分子结合时才会发射荧光信号，即使在浓度很低时，避免不准确测量带来的重复工作。发射通道：绿光510–580nm、红光665–720nm。选择蓝光激发时，读取绿色或远红外通道的荧光。当激发光为红光时，只读取远红外通道的荧光。Fluorescence detection unit A (5) and fluorescence detection unit B (6) are respectively vertically located on both sides of the sample chamber (11) and the excitation light path, welded on the circuit board (17), and embedded in the light transmission channel (12). High-precision and high-sensitivity photodiodes are used to receive and detect the intensity of the fluorescence signal emitted by the sample to be tested in the sample tank (11), which can improve the accuracy of fluorescence detection, and the measurement range is 300-1,000nm. These fluorochromes emit fluorescent signals only when bound to these target molecules, even at very low concentrations, avoiding duplication of effort due to inaccurate measurements. Emission channel: green light 510–580nm, red light 665–720nm. When blue light is selected for excitation, read fluorescence in the green or far-infrared channel. When the excitation light is red light, only the fluorescence in the far-infrared channel is read.
光传输通道(12)为封闭式光传输通道，底部通过膨胀螺栓固定于电路板(17)上，将光路各个组件进行了很有效的保护，收集激发光源A(1)、激发光源B(2)发射光，分别通过滤光片A(7)、滤光片B(8)到达样品槽(11)，并收集激发的荧光信号，通过滤光片C(9)、滤光片D(10)到达荧光探测单元A(5)、荧光探测单元B(6)。能有效消除外界杂散光的干扰，而且可以阻止灰尘对光路组件的污染，使光传输更洁净，大大提高了检测的准确性及灵敏度。The optical transmission channel (12) is a closed optical transmission channel, the bottom of which is fixed on the circuit board (17) by expansion bolts, and each component of the optical path is effectively protected, and the excitation light source A (1) and the excitation light source B (2 ) to emit light, respectively pass through filter A (7), filter B (8) to reach the sample chamber (11), and collect the excited fluorescence signal, pass through filter C (9), filter D (10 ) to the fluorescent detection unit A (5) and the fluorescent detection unit B (6). It can effectively eliminate the interference of external stray light, and can prevent dust from polluting optical path components, making light transmission cleaner, and greatly improving the accuracy and sensitivity of detection.
滤光片A(7)、滤光片B(8)分别位于激发光源A(1)、激发光源B(2)与样品槽(11)之间，嵌于光传输通道(12)内，用于滤除激发光之外的杂散光；所述滤光片C(9)、滤光片D(10)分别位于样品槽(11)与荧光探测单元A(5)、荧光探测单元B(6)之间，用于滤除荧光之外的杂散光。Optical filter A (7) and optical filter B (8) are respectively located between the excitation light source A (1), excitation light source B (2) and the sample groove (11), embedded in the light transmission channel (12), and used The filter C (9) and the filter D (10) are respectively located in the sample tank (11) and the fluorescence detection unit A (5) and the fluorescence detection unit B (6) to filter out stray light other than the excitation light. ) to filter out stray light other than fluorescence.
信号检测单元(13)焊接在电路板(17)上，用于将荧光探测单元A(5)、荧光探测单元B(6)转换的电信号，进行前置放大、解调、滤波、采集。The signal detection unit (13) is welded on the circuit board (17), and is used for preamplifying, demodulating, filtering and collecting the electrical signals converted by the fluorescence detection unit A (5) and the fluorescence detection unit B (6).
主控单元(14)焊接在电路板(17)上，采用的双核处理器，5秒内快速准确地定量DNA，RNA和蛋白。将结果数据显示到液晶显示屏(16)，并存储。The main control unit (14) is welded on the circuit board (17), adopts a dual-core processor, and can quickly and accurately quantify DNA, RNA and protein within 5 seconds. The resulting data is displayed on a liquid crystal display (16) and stored.
激发光控制单元(15)焊接在电路板(17)上，用于控制激发光源A(1)、激发光源B(2)输出光强度。The excitation light control unit (15) is welded on the circuit board (17), and is used to control the output light intensity of the excitation light source A (1) and the excitation light source B (2).
液晶显示单元(16)镶嵌于壳体(18)上表面，通过通讯线缆与电路板(17)连接，用于人机交互，功能选择，数据存储。The liquid crystal display unit (16) is inlaid on the upper surface of the housing (18), connected to the circuit board (17) through a communication cable, and used for human-computer interaction, function selection, and data storage.
样品槽盖(19)位于样品槽(11)之上，采用不透光材料制作，检测时将其盖严，用于减少环境中的杂散光对实验结果的影响。The sample tank cover (19) is located on the sample tank (11), and is made of opaque material, which is tightly covered during detection to reduce the influence of stray light in the environment on the experimental results.
屏蔽罩(20)罩位于光传输通道(12)之上，采用薄铝材料，用于屏蔽电磁信号干扰，可有效提高信噪比。The shielding cover (20) is located on the optical transmission channel (12), and is made of thin aluminum material, which is used for shielding electromagnetic signal interference and can effectively improve the signal-to-noise ratio.
检测方法是：The detection method is:
步骤一：将荧光试剂pico orange与磷酸盐缓冲液以1：198-200混匀；Step 1: Mix the fluorescent reagent pico orange with phosphate buffer at a ratio of 1:198-200;
步骤二：分别取两个不同浓度的牛血清白蛋白与磷酸盐缓冲液以1:18-20混匀；Step 2: Take two different concentrations of bovine serum albumin and phosphate buffer and mix them at a ratio of 1:18-20;
步骤三：分别将混合后的不同浓度的牛血清白蛋白溶液放入装置中，室温孵育15分钟，进行两点定标，绘制标准曲线(蛋白浓度-荧光强度)；Step 3: Put mixed bovine serum albumin solutions of different concentrations into the device, incubate at room temperature for 15 minutes, perform two-point calibration, and draw a standard curve (protein concentration-fluorescence intensity);
步骤四：得到标准曲线后，取待测样品与磷酸盐缓冲液以1-20:180-199比例混匀；Step 4: After obtaining the standard curve, mix the sample to be tested with phosphate buffer at a ratio of 1-20:180-199;
步骤五：放入样品槽内，室温孵育15分钟，读取与样本荧光强度相对应的蛋白浓度。Step 5: Put it into the sample tank, incubate at room temperature for 15 minutes, and read the protein concentration corresponding to the fluorescence intensity of the sample.
本发明的有益效果是：这些荧光染料只有与这些蛋白靶分子结合时才会发射荧光信号，即便是在浓度很低时也能发出荧光信号。相比传统的紫外吸光法更加灵敏、更准确，尤其克服了DNA核酸紫外吸收的干扰。因此本发明装置特别适用于以下情况：当样品十分稀有且难以处理；样品中蛋白含量很少；因此使用本发明方法，荧光染料与蛋白分子特异结合，将大大提升样品检测的灵敏度和准确性。The beneficial effect of the present invention is that these fluorescent dyes can emit fluorescent signals only when they combine with these protein target molecules, even when the concentration is very low, they can also emit fluorescent signals. Compared with the traditional ultraviolet absorption method, it is more sensitive and accurate, especially overcoming the interference of DNA nucleic acid ultraviolet absorption. Therefore, the device of the present invention is especially suitable for the following situations: when the sample is very rare and difficult to handle; the protein content in the sample is very small; therefore, using the method of the present invention, the specific combination of fluorescent dyes and protein molecules will greatly improve the sensitivity and accuracy of sample detection.
附图说明Description of drawings
图1是本发明外观整体结构图。Fig. 1 is the overall structural diagram of the appearance of the present invention.
图2是本发明整体结构局剖图。Fig. 2 is a partial sectional view of the overall structure of the present invention.
图3是本发明结构及工作原理示意图。Fig. 3 is a schematic diagram of the structure and working principle of the present invention.
图4本发明荧光强度-蛋白浓度标准曲线图。Fig. 4 is a standard curve diagram of fluorescence intensity-protein concentration of the present invention.
具体实施方式detailed description
实施例1Example 1
下面结合附图和实施例对本发明专利做进一步详细说明。Below in conjunction with accompanying drawing and embodiment, the patent of the present invention is described in further detail.
如图所示，1是激发光源A、2是激发光源B、3是激发光探测单元A、4是激发光探测单元B、5是荧光探测单元A、6是荧光探测单元B、7是滤光片A、8是滤光片B、9是滤光片C、10是滤光片D、11是样品槽、12光传输通道、13是信号检测单元、14是主控单元、15是激发光控制单元、16是液晶显示单元、17是电路板、18是壳体、19是样品槽盖、20是屏蔽罩。As shown in the figure, 1 is the excitation light source A, 2 is the excitation light source B, 3 is the excitation light detection unit A, 4 is the excitation light detection unit B, 5 is the fluorescence detection unit A, 6 is the fluorescence detection unit B, 7 is the filter Optical sheet A, 8 is optical filter B, 9 is optical filter C, 10 is optical filter D, 11 is sample slot, 12 is optical transmission channel, 13 is signal detection unit, 14 is main control unit, 15 is excitation Light control unit, 16 is a liquid crystal display unit, 17 is a circuit board, 18 is a casing, 19 is a sample tank cover, and 20 is a shielding cover.
所述激发光源A(1)位于样品槽(11)左侧，激发光源B(2)位于样品槽(11)右侧，分别焊接在电路板(17)上，嵌于光传输通道(12)内，用于激发样品中的荧光物质。The excitation light source A (1) is located on the left side of the sample slot (11), and the excitation light source B (2) is located on the right side of the sample slot (11), respectively welded on the circuit board (17), embedded in the light transmission channel (12) Inside, used to excite fluorescent substances in the sample.
所述激发光探测单元A(3)位于激发光源A(1)与样品槽(11)之间，激发光探测单元B(4)位于激发光源B(2)与样品槽(11)之间，两者高度均低于激发光源，焊接于电路板(17)上，嵌于光传输通道(12)内，保证激发光可以照射到样品槽(11)内样品，用于对激发光的状态进行实时监测，确认激发光是否正常工作、光强度是否稳定；The excitation light detection unit A (3) is located between the excitation light source A (1) and the sample chamber (11), the excitation light detection unit B (4) is located between the excitation light source B (2) and the sample chamber (11), Both heights are lower than the excitation light source, are welded on the circuit board (17), embedded in the light transmission channel (12), to ensure that the excitation light can be irradiated to the sample in the sample groove (11), and are used to monitor the state of the excitation light. Real-time monitoring to confirm whether the excitation light is working normally and whether the light intensity is stable;
所述荧光探测单元A(5)、荧光探测单元B(6)分别位于以样品槽(11)为垂足，与激发光光路垂直的两侧，焊接于电路板(17)上，嵌于光传输通道(12)内。接收并检测由样本槽(11)内待测样本发出的荧光信号强度，均采用高精度、高灵敏度的光电二极管，可提高荧光检测精度，测量范围为300-1,000nm。这些荧光染料只有与这些靶分子结合时才会发射荧光信号，即使在浓度很低时，避免不准确测量带来的重复工作。发射通道：绿光510–580nm、红光665–720nm。选择蓝光激发时，读取绿色或远红外通道的荧光。当激发光为红光时，只读取远红外通道的荧光。The fluorescence detection unit A (5) and the fluorescence detection unit B (6) are respectively located on the two sides perpendicular to the optical path of the excitation light with the sample groove (11) as the foot, are welded on the circuit board (17), and embedded in the light In the transmission channel (12). High-precision and high-sensitivity photodiodes are used to receive and detect the intensity of the fluorescence signal emitted by the sample to be tested in the sample tank (11), which can improve the accuracy of fluorescence detection, and the measurement range is 300-1,000nm. These fluorochromes emit fluorescent signals only when bound to these target molecules, even at very low concentrations, avoiding duplication of effort due to inaccurate measurements. Emission channel: green light 510–580nm, red light 665–720nm. When blue light is selected for excitation, read fluorescence in the green or far-infrared channel. When the excitation light is red light, only the fluorescence in the far-infrared channel is read.
所述光传输通道(12)，为封闭式光传输通道，底部通过膨胀螺栓固定于电路板(17)上，将光路各个组件进行了很有效的保护，收集激发光源A(1)、激发光源B(2)发射光，分别通过滤光片A(7)、滤光片B(8)到达样品槽(11)，并收集激发的荧光信号，通过滤光片C(9)、滤光片D(10)到达荧光探测单元A(5)、荧光探测单元B(6)。能有效消除外界杂散光的干扰，而且可以阻止灰尘对光路组件的污染，使光传输更洁净，大大提高了检测的准确性及灵敏度。The optical transmission channel (12) is a closed optical transmission channel, the bottom of which is fixed on the circuit board (17) by expansion bolts, which effectively protects each component of the optical path, collects the excitation light source A (1), the excitation light source Light emitted by B(2) reaches the sample chamber (11) through filter A(7) and filter B(8) respectively, and collects the excited fluorescence signal, passes through filter C(9), filter D (10) reaches the fluorescence detection unit A (5) and the fluorescence detection unit B (6). It can effectively eliminate the interference of external stray light, and can prevent dust from polluting optical path components, making light transmission cleaner, and greatly improving the accuracy and sensitivity of detection.
所述滤光片A(7)、滤光片B(8)分别位于激发光源A(1)、激发光源B(2)与样品槽(11)之间，嵌于光传输通道(12)内，用于滤除激发光之外的杂散光；所述滤光片C(9)、滤光片D(10)分别位于样品槽(11)与荧光探测单元A(5)、荧光探测单元B(6)之间，用于滤除荧光之外的杂散光。The optical filter A (7) and the optical filter B (8) are respectively located between the excitation light source A (1), the excitation light source B (2) and the sample tank (11), embedded in the light transmission channel (12) , used to filter out stray light other than excitation light; said filter C (9), filter D (10) are respectively located in the sample tank (11) and fluorescence detection unit A (5), fluorescence detection unit B Between (6), used to filter out stray light other than fluorescence.
所述信号检测单元(13)焊接于电路板(17)上，用于将荧光探测单元A(5)、荧光探测单元B(6)转换的电信号，进行前置放大、解调、滤波、采集。The signal detection unit (13) is welded on the circuit board (17), and is used to perform preamplification, demodulation, filtering, collection.
所述主控单元(14)焊接于电路板(17)上，采用的双核处理器，5秒内快速准确地定量DNA，RNA和蛋白。将结果数据显示到液晶显示屏(16)，并存储。The main control unit (14) is welded on the circuit board (17), adopts a dual-core processor, and can quickly and accurately quantify DNA, RNA and protein within 5 seconds. The resulting data is displayed on a liquid crystal display (16) and stored.
所述激发光控制单元(15)焊接于电路板(17)上，用于控制激发光源A(1)、激发光源B(2)输出光强度。The excitation light control unit (15) is welded on the circuit board (17), and is used to control the output light intensity of the excitation light source A (1) and the excitation light source B (2).
所述液晶显示单元(16)镶嵌于壳体(18)上表面，通过通讯线缆与电路板(17)连接，用于人机交互，功能选择，数据存储。The liquid crystal display unit (16) is inlaid on the upper surface of the housing (18), connected to the circuit board (17) through a communication cable, and used for human-computer interaction, function selection, and data storage.
所述样品槽盖(19)位于样品槽(11)之上，采用不透光材料制作，检测时将其盖严，用于减少环境中的杂散光对实验结果的影响。The sample tank cover (19) is located on the sample tank (11) and is made of opaque material. It is tightly covered during detection to reduce the influence of stray light in the environment on the experimental results.
所述屏蔽罩(20)罩于光传输通道(12)之上，采用薄铝材料，用于屏蔽电磁信号干扰，可有效提高信噪比。The shielding cover (20) is covered on the optical transmission channel (12), and is made of thin aluminum material for shielding electromagnetic signal interference, which can effectively improve the signal-to-noise ratio.
本实例中，双激发光源包括蓝光LED和红光LED，可以根据需要手动选择。当选择蓝光激发时，该装置读取绿色或远红外通道的荧光。当激发光为红光时，只读取远红外通道的荧光。In this example, the dual excitation light source includes a blue LED and a red LED, which can be manually selected as required. The device reads fluorescence in the green or far-infrared channel when blue light excitation is selected. When the excitation light is red light, only the fluorescence in the far-infrared channel is read.
本实例中，上述激发光探测单元，可以对激发光的状态进行实时监测并由激光输出单元进行补偿。当未探测到激发光则在显示屏幕上提示报警；当激发光强度存在偏差时，则进行激发光补偿。In this example, the above-mentioned excitation light detection unit can monitor the state of the excitation light in real time and compensate it by the laser output unit. When the excitation light is not detected, an alarm will be prompted on the display screen; when there is a deviation in the intensity of the excitation light, the excitation light compensation will be performed.
本实例中，光传输通道将光路各个组件进行了很有效的保护，并能有效消除外界杂散光的干扰，而且可以阻止灰尘对光路组件的污染，使光传输更洁净，大大提高了检测的准确性及灵敏度。In this example, the optical transmission channel effectively protects each component of the optical path, and can effectively eliminate the interference of external stray light, and can prevent dust from polluting the optical path components, making the optical transmission cleaner and greatly improving the accuracy of detection performance and sensitivity.
本实例中，带通滤光片针对两种激发光源，分别采用了钬玻璃和钕镨玻璃材质的滤光片，提高激发光波的准确度。In this example, the band-pass filters are respectively made of holmium glass and dodmium-praseodymium glass for the two kinds of excitation light sources, so as to improve the accuracy of the excitation light waves.
本实例中，荧光探测单元采用高精度、高灵敏度的光电二极管，提高了荧光检测精度，由信号检测单元将光电二极管转换的电信号发送到主控单元进行数据处理，并显示与显示屏幕上，且数据可以保存。In this example, the fluorescence detection unit uses a high-precision, high-sensitivity photodiode, which improves the accuracy of fluorescence detection. The signal detection unit sends the electrical signal converted by the photodiode to the main control unit for data processing, and displays it on the display screen. And the data can be saved.
本实例中，光传输通道将光路各个组件进行了很有效的保护，并能有效消除外界杂散光的干扰，而且可以阻止灰尘对光路组件的污染，使光传输更洁净，大大提高了检测的准确性及灵敏度。In this example, the optical transmission channel effectively protects each component of the optical path, and can effectively eliminate the interference of external stray light, and can prevent dust from polluting the optical path components, making the optical transmission cleaner and greatly improving the accuracy of detection performance and sensitivity.
本实例中，光传输通道将光路各个组件进行了很有效的保护，并能有效消除外界杂散光的干扰，而且可以阻止灰尘对光路组件的污染，使光传输更洁净，大大提高了检测的准确性及灵敏度。In this example, the optical transmission channel effectively protects each component of the optical path, and can effectively eliminate the interference of external stray light, and can prevent dust from polluting the optical path components, making the optical transmission cleaner and greatly improving the accuracy of detection performance and sensitivity.
本实例中，荧光微量蛋白的检测装置，其使用方法包括如下步骤：In this example, the method for using the detection device for fluorescent microproteins includes the following steps:
步骤一：将1ul荧光试剂pico orange与磷酸盐缓冲液199ul混匀；Step 1: Mix 1ul fluorescent reagent pico orange with 199ul phosphate buffer;
步骤二：取190μL磷酸盐缓冲液，分别加入10μL 0.1mg/ml牛血清白蛋白、1mg/ml牛血清白蛋白混匀；Step 2: Take 190 μL of phosphate buffer, add 10 μL of 0.1 mg/ml bovine serum albumin and 1 mg/ml bovine serum albumin, and mix well;
步骤三：分别将混合后的0.1mg/ml牛血清白蛋白和1mg/ml牛血清白蛋白放入装置中，室温孵育15分钟，进行两点定标，绘制标准曲线(蛋白浓度-荧光强度)；Step 3: Put the mixed 0.1mg/ml bovine serum albumin and 1mg/ml bovine serum albumin into the device respectively, incubate at room temperature for 15 minutes, perform two-point calibration, and draw a standard curve (protein concentration-fluorescence intensity) ;
步骤四：得到标准曲线后，取待测样品10μL与190μL磷酸盐缓冲液混匀；Step 4: After obtaining the standard curve, take 10 μL of the sample to be tested and mix with 190 μL phosphate buffer;
步骤五：放入本发明装置样品槽内，室温孵育15分钟，读取与样本荧光强度相对应的蛋白浓度。Step 5: Put it into the sample tank of the device of the present invention, incubate at room temperature for 15 minutes, and read the protein concentration corresponding to the fluorescence intensity of the sample.
| Application Number | Priority Date | Filing Date | Title |
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| CN201710749306.0ACN107576802A (en) | 2017-08-28 | 2017-08-28 | A kind of detection means and detection method of fluorescence micro albumen |
| Application Number | Priority Date | Filing Date | Title |
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| CN201710749306.0ACN107576802A (en) | 2017-08-28 | 2017-08-28 | A kind of detection means and detection method of fluorescence micro albumen |
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| CN107576802Atrue CN107576802A (en) | 2018-01-12 |
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| CN201710749306.0APendingCN107576802A (en) | 2017-08-28 | 2017-08-28 | A kind of detection means and detection method of fluorescence micro albumen |
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